Lactic Acid Patents (Class 435/139)
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Publication number: 20140227745Abstract: The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.Type: ApplicationFiled: February 7, 2014Publication date: August 14, 2014Applicant: William Marsh Rice UniversityInventors: Ka-Yiu SAN, George N. BENNETT, Hui WU
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METHOD FOR ENHANCING THE FERMENTATIVE POTENTIAL AND GROWTH RATE OF MICROORGANISMS UNDER ANAEROBIOSIS
Publication number: 20140227749Abstract: The present invention concerns a method for enhancing the growth rate, the biomass production and/or the fermentative potential of an anaerobic microorganism, comprising the steps consisting in: (a) modifying said anaerobic microorganism by inactivating the ohrR gene, the ohrR gene product or homolog thereof; (b) culturing the modified anaerobic microorganism obtained in step (a) under low-ORP anaerobic conditions.Type: ApplicationFiled: June 5, 2012Publication date: August 14, 2014Applicants: Universite d'Avignon et des Pays de Vaucluse, Commissariat a l'energie atomique et aux ene altInventors: Jean Armengaud, Geremy Clair, Catherine Duport -
Patent number: 8802403Abstract: Disclosed herein is a genetic modification of moderately thermophilic Bacillus species that are facultative anaerobic and homolactic. The method includes introducing DNA cloned in a thermosensitive plasmid system containing a pSH71 replicon or a homologue thereof into cells of a moderately thermophilic Bacillus species that is facultative anaerobic and homolactic; culturing the cells on a selective medium at a permissive temperature to select transformed cells; culturing the transformed cells on a selective medium at a non-permissive temperature to select transformed cells capable of growing on the selective medium at the non-permissive temperature. The method can modify the Bacilli for R-lactic acid production, production of other organic acids than lactic acid, alcohol, enzymes, amino acids, and vitamins. The Bacillus species may be modified by replacing the S-lactate dehydrogenase gene by a DNA construct including a DNA sequence encoding R-lactate dehydrogenase.Type: GrantFiled: June 27, 2013Date of Patent: August 12, 2014Assignee: Purac Biochem B.V.Inventors: Richard Van Kranenburg, Mariska Van Hartskamp, Eelco Anthonius Johannes Heintz, Esther Johanna Geertruda Van Mullekom, Jurgen Snelders
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Publication number: 20140220635Abstract: The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 17, 2012Publication date: August 7, 2014Applicant: Novozymes A/SInventors: Carsten Andersen, Signe Larsen, Esben Peter Friis, Pernille Ollendorff Micheelsen, Anders Viksoe Nielsen, Randall Deinhammer, Xinyu Shen
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Publication number: 20140220647Abstract: A recombinant microorganism comprising a lactic acid (LA) transporter, wherein the expression of the LA transporter in the recombinant microorganism is increased relative to a parent microorganism, and a method of producing lactic acid using same.Type: ApplicationFiled: February 5, 2014Publication date: August 7, 2014Applicant: Samsung Electronics Co., Ltd.Inventors: Jae-young KIM, Jin-kyu Kang, Chang-duk Kang, Sung-soo Kim, Jae-chan Park, Byung-jo Yu, Ju-young Lee, Hui-sub Lim
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Patent number: 8795993Abstract: A process is described for producing fermentable sugars derivable from biomass that contains polysaccharide, such as cellulose, made increasingly accessible as a substrate for enzymatic degradation or other methods of depolymerization. These fermentable sugars are subsequently able to be fermented to produce various target chemicals, such as alcohols, aldehydes, ketones or acids.Type: GrantFiled: February 3, 2010Date of Patent: August 5, 2014Assignee: Hercules IncorporatedInventors: Herbert T. Conner, Patrick J. Cowan, John C. Gast
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Publication number: 20140212932Abstract: The present invention relates to enzyme compositions comprising a polypeptide having cellobiohydrolase II activity, a polypeptide having xylanase activity, and one or more cellulolytic proteins and their use in the degradation or conversion of cellulosic material.Type: ApplicationFiled: April 8, 2014Publication date: July 31, 2014Applicant: Novozymes, Inc.Inventors: Kimberly Brown, Eric Abbate
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Publication number: 20140212933Abstract: The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.Type: ApplicationFiled: July 9, 2012Publication date: July 31, 2014Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Xuan Wang, Elliot N. Miller, Lorraine P. Yomano, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20140212937Abstract: The invention provides a microbubble generation system with increased efficiency and flexibility compared to known systems. Further, the invention provides a method of microbubble generation. In particular, invention relates to increasing the efficiency of a fermentation reaction by reducing bubble size and increasing gas absorption into a liquid fermentation broth.Type: ApplicationFiled: January 28, 2014Publication date: July 31, 2014Applicant: LanzaTech New Zealand LimitedInventor: Xueliang Li
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Publication number: 20140206901Abstract: The invention relates to methods for the production of chemical compounds, particularly but not exclusively ethanol, by microbial fermentation. Also described are genetically modified micro-organisms capable of using carbon monoxide to produce one or more products, particularly but not exclusively ethanol as a main product, and producing a reduced amount or substantially no 2,3-butanediol and/or a precursor thereof.Type: ApplicationFiled: January 31, 2013Publication date: July 24, 2014Inventors: Michael Koepke, Shilpa Nagaraju, Wendy Yiting Chen
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Publication number: 20140206046Abstract: Described herein are improved methods of degrading or converting cellulosic material into fermentable sugars using dithionite. Also described are improved methods of fermentation in the presence of dithionite.Type: ApplicationFiled: September 7, 2012Publication date: July 24, 2014Inventors: Hui Xu, Ye Chen, Xin Li, Mark Stevens, Zhengfang Kang
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Publication number: 20140206035Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: ApplicationFiled: August 24, 2012Publication date: July 24, 2014Inventors: Tianqi Sun, Ming Li
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Publication number: 20140193853Abstract: Bioreactors are provided that include a vessel and a jet mixer disposed in the vessel. Methods that utilize the bioreactors are provided, involving placing a microorganism or cells and a fluid medium in the bioreactor.Type: ApplicationFiled: March 4, 2014Publication date: July 10, 2014Applicant: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman
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Publication number: 20140186884Abstract: This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.Type: ApplicationFiled: July 23, 2013Publication date: July 3, 2014Applicant: BP Corporation North Americca Inc.Inventors: David Neal Nunn, JR., Peter Luginbuhl, Ling Li, Adam Martin Burja, John Peter Flash Bartnek
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Publication number: 20140178937Abstract: A method of producing a sugar liquid with a cellulose-containing biomass as a raw material includes (1) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution, and (2) filtering the aqueous sugar solution obtain in (1) through an ultrafiltration membrane having a molecular weight cutoff of 600 to 2,000 to remove a fermentation inhibitor(s) into the permeate side and collect a sugar liquid from the feed side.Type: ApplicationFiled: July 27, 2012Publication date: June 26, 2014Applicant: Toray Industries, Inc.Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada
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Patent number: 8759045Abstract: A method of producing lactic acid by separating lactic acid produced in a culture solution by microbial fermentation, comprising: a step (A) of filtering the culture solution through a nano-filtration membrane; and a step (B) of distilling a lactic-acid-containing solution produced in step (A) under a pressure of 1 Pa to atmospheric pressure (inclusive) at a temperature of 25 to 200° C. (inclusive) to recover lactic acid.Type: GrantFiled: December 7, 2012Date of Patent: June 24, 2014Assignee: Toray Industries, Inc.Inventors: Masateru Ito, Shin-ichi Minegishi, Eri Shimizu, Kenji Sawai, Yohito Ito, Hideki Sawai, Katsushige Yamada
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Publication number: 20140170719Abstract: The present invention relates generally to compositions and methods for producing lactic acid using a lactic acid producing microorganism. More specifically, the present invention relates to methods for producing lactic acid with high yield, high concentration and high volumetric productivity through biological fermentation by Enterococcus faecalis, or recombinant microorganisms transformed to produce lactate dehydrogenase using the lactate dehydrogenase-encoding genes derived from E. faecalis.Type: ApplicationFiled: December 17, 2013Publication date: June 19, 2014Applicant: South Dakota Board of RegentsInventors: Lew P. Christopher, Mohanraj Subramanian, Suvarna Naga Lavanya Talluri
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Publication number: 20140162312Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.Type: ApplicationFiled: August 2, 2012Publication date: June 12, 2014Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Rene Marcel De Jong
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Publication number: 20140162328Abstract: A process for increasing the production of a glycolytic intermediate and/or an organic compound as defined herein by a cell that is able to express a nucleic acid molecule, wherein the expression of the nucleic acid molecule gives the cell the ability to increase the production of a glycolytic intermediate and/or the organic compound and wherein the carbon substrate is sugar and/or protein.Type: ApplicationFiled: December 12, 2012Publication date: June 12, 2014Inventor: Trent Nguyen
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Publication number: 20140150334Abstract: This invention provides processes to convert biomass into energy-dense biomass for combustion, alone or in combination with another solid fuel. Some embodiments provide processes for producing energy-dense biomass from cellulosic biomass, comprising extracting the feedstock with steam and/or hot water to produce an extract liquor containing hemicellulosic oligomers, dissolved lignin, and cellulose-rich solids; separating the extract liquor, to produce dewatered cellulose-rich solids; hydrolyzing the dewatered cellulose-rich solids, thereby removing a portion of the cellulose, to produce intermediate solids (with higher energy density) and a hydrolysate; drying the intermediate solids to produce energy-dense biomass; and optionally recovering fermentable sugars from the hydrolysate. The energy-dense biomass may be pelletized into biomass pellets, which may have a similar energy density as torrefied pellets from wood.Type: ApplicationFiled: February 5, 2014Publication date: June 5, 2014Applicant: API Intellectual Property Holdings, LLCInventors: Theodora RETSINA, Vesa PYLKKANEN
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Publication number: 20140154759Abstract: This invention provides processes and apparatus to convert biomass, including wood and agricultural residues, into low-ash biomass pellets for combustion, alone or in combination with another solid fuel. Some embodiments provide processes for producing low-ash biomass from cellulosic biomass, comprising providing an aqueous extraction solution with acetic acid; extracting the feedstock to produce an extract liquor containing soluble ash, hemicellulosic oligomers, acetic acid, dissolved lignin, and cellulose-rich solids; dewatering and drying the cellulose-rich, lignin-rich solids to produce a low-ash biomass; hydrolyzing the hemicellulosic oligomers to produce hemicellulosic sugars, wherein additional acetic acid is generated; removing a vapor stream comprising vaporized acetic acid from the extract; and recycling the vapor or its condensate to provide some starting acetic acid for the extraction solution. The disclosed processes can produce clean power from biomass.Type: ApplicationFiled: February 5, 2014Publication date: June 5, 2014Applicant: API Intellectual Property Holdings, LLCInventors: Theodora RETSINA, Vesa PYLKKANEN
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Publication number: 20140154749Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.Type: ApplicationFiled: May 24, 2013Publication date: June 5, 2014Applicant: XYLECO, INC.Inventor: Marshall Medoff
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Patent number: 8741599Abstract: A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproducType: GrantFiled: November 27, 2006Date of Patent: June 3, 2014Assignee: BASF SEInventors: Markus Pompejus, Stephan Freyer, Markus Lohscheidt, Oskar Zelder, Matthias Boy
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Patent number: 8741365Abstract: The present disclosure relates in one embodiment to a method of producing a fermented food, wherein a raw food material is combined with a starter culture for fermentation, wherein at least one microorganism of the genus Bacteroides and/or at least one microorganism having at least a cell surface structure which corresponds to a human carbohydrate structure is added. Further disclosed are processing aids and fermented foods containing respective microorganisms.Type: GrantFiled: May 13, 2009Date of Patent: June 3, 2014Assignee: Glycotope GmbHInventors: Steffen Goletz, Philippe Ulsemer
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Patent number: 8741610Abstract: The present invention provides, inter alia, an improvement in the production of a desired product from yeast, wherein the growth rate and fermentation rate of the yeast are maintained at excellent levels. The present invention also provides, inter alia, for the introduction of a foreign gene into a yeast host cell, wherein the foreign gene encodes an enzyme involved in the production of a desired product. The yeast may also constitutively express a HAP4 gene, or a homologous gene thereof, to significantly improve the ability of the yeast to produce the desired product while maintaining its growth rate and fermentation rate. In some embodiments, a yeast mutant is used which is a mutant strain having lowered alcohol productivity vis-à-vis a wild-type strain.Type: GrantFiled: April 23, 2009Date of Patent: June 3, 2014Assignee: Toyota Jidosha Kabushiki KaishaInventors: Toru Onishi, Nobuki Tada, Hibiki Matsushita, Noriko Yasutani, Nobuhiro Ishida, Takashi Shimamura
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Publication number: 20140141475Abstract: A process for treating a mixture of R,R- and S,S-lactide is provided. The process involves contacting the mixture with an aliphatic alcohol and an enzyme in the presence of a ketone solvent to produce a mixture comprising aliphatic ester of lactic acid corresponding to one lactide enantiomer, and the aliphatic ester of lactyllactic acid corresponding to the other lactide enantiomer. Also provided are processes for the production of S-lactic acid, S,S-lactide, poly-S-lactic acid, R-lactic acid, R,R-lactide, poly-R-lactic acid and stereocomplex polylactic acid.Type: ApplicationFiled: August 21, 2012Publication date: May 22, 2014Applicant: Plaxica LimitedInventors: Edward Leslie Marshall, Jade Jocelyn Afriye Osei Tutu, Stephen Alexander Calder Smith
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Publication number: 20140141471Abstract: The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material.Type: ApplicationFiled: May 18, 2012Publication date: May 22, 2014Applicant: Novozymes, Inc.Inventors: Feng Xu, William Widner, Ani Tejirian
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Publication number: 20140141473Abstract: Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.Type: ApplicationFiled: April 20, 2012Publication date: May 22, 2014Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Panagiotis Sarantinopoulos, Wilbert Herman Marie Heijne, Aldo Greeve
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Patent number: 8728762Abstract: The invention relates to a method for producing at least one organic compound comprising at least 3 C-atoms or at least 2 C-atoms and at least 1 N-atom by fermentation. Said method comprises the following steps: i) a starch source is ground in order to obtain a grinding material which contains at least one part of the non-starch containing solid components of the starch source; ii) the grinding material is suspended in an aqueous liquid in an amount such that the dry mass content in the suspension is at least 45 wt.Type: GrantFiled: November 27, 2006Date of Patent: May 20, 2014Assignee: BASF SEInventors: Matthias Boy, Stephan Freyer
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Patent number: 8728777Abstract: The present invention relates to novel stress-resistant bacteria and the uses thereof. More specifically, the invention relates to isolated stress-resistant bacteria having advantageous properties for the production of organic acids or alcohols in various culture conditions. The invention also relates to methods of producing organic acids or alcohols using said bacteria, particularly from biomass.Type: GrantFiled: May 12, 2010Date of Patent: May 20, 2014Assignees: Deinove, Centre Naitonal de la Recherche Scientifique, Universite Montpellier IInventors: Jacques Biton, Cathy Isop
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Publication number: 20140134672Abstract: Bioreactors, and particularly, photobioreactors having a reactor chamber and surge driver, and methods for using these devices, for example, for the production of carbon-based products are provided. The reactor chamber provides a housing for microorganisms and culture medium. The surge driver produces a surge of the microorganisms and/or culture medium in the reactor chamber.Type: ApplicationFiled: July 5, 2012Publication date: May 15, 2014Inventors: Max B. Tuttman, David A. St. Angelo
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Publication number: 20140128635Abstract: Disclosed is the biological production of lactic acid using a microorganism. A transformant capable of producing lactic acid of high optical purity at high yield, a method for the preparation thereof, and a method for producing lactic acid in a convenient and economically beneficial manner using the same are provided. The Zymomonas mobilis transformant can produce lactic acid of high optical purity at high yield without a stringent limitation to production conditions and a regulation of the intracellular metabolism pathway. Because it requires no additional separation and purification steps, the use of the transformant allows the production of lactic acid in a short process, resulting in a significant reduction in production cost, and avoiding the environment problems caused by precipitate wastes, which brings about environmental issues.Type: ApplicationFiled: February 23, 2011Publication date: May 8, 2014Applicant: MACROGEN INC.Inventors: Jae-Young Kim, Sang-Heum Shin, Hyon-Yong Chong, Kap-Seok Yang, Jeong-Sun Seo
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Patent number: 8715972Abstract: Provided is a lactic acid bacterium capable of homolactic fermentation using a pentose as a substrate, the lactic acid bacterium utilizing a pentose, and in which a phosphoketolase pathway is blocked and a pentose phosphate pathway is activated. Also provided is a method for producing lactic acid from a pentose using the lactic acid bacterium and a method for preparing the lactic acid bacterium.Type: GrantFiled: February 15, 2010Date of Patent: May 6, 2014Assignees: Bio-energy Corporation, Kansai Chemical Engineering Co., Ltd.Inventors: Akihiko Kondo, Kenji Okano, Hideo Noda
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Publication number: 20140120591Abstract: An isolated biologically pure culture of Clostridium tyrobutyricum ITRI04001 or an isolated biologically pure culture of Clostridium tyrobutyricum having the genotypic characteristics of ITRI04001 useful for syngas fermentation. Volatile free acids are produced by a method comprising culturing a microorganism having the genotypic characteristics of ITRI04001 in a medium; providing at least one substrate comprising at least one carbon source chosen from CO and CO2 to the microorganism; and recovering at least one free volatile free acid. Syngas can be the at least one substrate in these processes for producing volatile free acids.Type: ApplicationFiled: October 2, 2013Publication date: May 1, 2014Applicant: Industrial Technology Research InstituteInventors: Chang-Chieh Chen, Yi-Te Chou, Shao-Wen Wu, Shi-Chan Tseng, Hsin-Tzu Kuo, Wan-Ting Ma
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Patent number: 8709771Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: GrantFiled: July 3, 2013Date of Patent: April 29, 2014Assignee: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman
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Publication number: 20140115739Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 11, 2013Publication date: April 24, 2014Inventors: Suchindra Maiyuran, Randall Kramer, Paul Harris
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Publication number: 20140106398Abstract: The present invention relates to a host cell deficient in an essential gene, comprising a vector, said vector comprising at least said essential gene and an autonomous replication sequence, wherein the host cell is a filamentous fungal cell. The invention also relates to a host cell deficient in an essential gene, comprising a vector, said vector comprising at least said essential gene and an autonomous replication sequence, wherein the host cell comprises a recombinant polynucleotide construct comprising a polynucleotide encoding a biological compound of interest or a compound involved in the synthesis of a biological compound of interest.Type: ApplicationFiled: March 12, 2012Publication date: April 17, 2014Applicant: DSM IP ASSETS B.V.Inventors: Roelof Ary Lans Bovenberg, Jan Andries Kornelis Willem Kiel, Thibaut José Wenzel, Alrik Pieter Los
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Publication number: 20140106420Abstract: Processes are disclosed for bioconversion processes in which a ME biocatalyst is surrounded by water-insoluble liquid during the bioconversion to facilitate one or more of mass transfer of substrate to and bioproduct from the biocatalyst and the separation and recovery of bioproduct from the water-insoluble liquid. The ME biocatalyst irreversibly retains microorganisms for the bioconversion and has, in its interior, an aqueous environment.Type: ApplicationFiled: December 13, 2013Publication date: April 17, 2014Applicant: Microvi Biotech, Inc.Inventors: Fatemeh RAZAVI-SHIRAZI, Mohammad Ali DORRI, Ameen RAZAVI
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Patent number: 8691538Abstract: Flat panel biofilm photobioreactor systems with a photosynthetic, autofermentative microorganism that forms a biofilm and methods for using the same to make metabolic intermediate compound(s) through photosynthesis and to convert metabolic intermediate compound(s) into chemical product(s) such as a biofuel or a feedstock through autofermentation.Type: GrantFiled: September 28, 2012Date of Patent: April 8, 2014Assignee: Algenol Biofuels Switzerland GmbHInventors: Benjamin Moll, Benjamin McCool, William Drake, William Porubsky, Ryan Adams, Heike Enke, Juliane Metzner, Karola Knuth
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Publication number: 20140093924Abstract: Flat panel biofilm photobioreactor systems with a photosynthetic, autofermentative microorganism that forms a biofilm and methods for using the same to make metabolic intermediate compound(s) through photosynthesis and to convert metabolic intermediate compound(s) into chemical product(s) such as a biofuel or a feedstock through autofermentation.Type: ApplicationFiled: September 28, 2012Publication date: April 3, 2014Applicant: ALGENOL BIOFUELS SWITZERLAND GMBHInventors: Benjamin Moll, Benjamin McCool, William Drake, William Porubsky, Ryan Adams
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Publication number: 20140096287Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 14, 2013Publication date: April 3, 2014Inventors: Kristian Krogh, Paul Harris
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Patent number: 8685703Abstract: A coryneform bacterium transformant prepared by transferring an exogenous gene which encodes a protein having a sugar transporter function into a coryneform bacterium capable of utilizing D-xylose.Type: GrantFiled: June 10, 2009Date of Patent: April 1, 2014Assignee: Research Institute of Innovative Technology for the EarthInventors: Hideaki Yukawa, Masayuki Inui
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Publication number: 20140087433Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation.Type: ApplicationFiled: November 21, 2012Publication date: March 27, 2014Applicant: XYLECO, INC.Inventor: Xyleco, Inc.
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Publication number: 20140087435Abstract: The present disclosure provides genetically engineered biocatalysts which enable intracellular assimilation of cellodextrin. The genetically engineered biocatalyst co-expresses a cellodextrin phosphorylase (CDP) gene and a cellobiose phosphorylase (CBP) gene. Further, the genetically engineered biocatalyst includes a first synthetic promoter used to express the cellodextrin phosphorylase (CDP) gene and a second synthetic promoter used to express the cellobiose phosphorylase (CBP) gene. Furthermore, the genetically engineered biocatalyst expresses one or more cellodextrin permeases. The intracellular assimilation includes hydrolysis and phosphorolysis mechanism. Further provided are methods of using the genetically engineered biocatalysts to generate various useful end-products including alcohol and lactic acid.Type: ApplicationFiled: August 13, 2013Publication date: March 27, 2014Applicant: Georgia Tech Research CorporationInventors: Rachel Ruizhen Chen, Hyun-Dong Shin, Ramanan Sekar
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Patent number: 8679800Abstract: The present invention provides: a lactic acid-producing Escherichia coli comprising at least one gene of a sucrose non-PTS gene group, including at least a sucrose hydrolase gene, provided that a combination of a repressor protein (cscR), a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) and a combination of a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) are excluded, wherein the lactic acid-producing Escherichia coli comprises a lactic acid production enhancing system provided by genetic recombination; and a lactic acid production method including producing lactic acid from a plant-derived sucrose-containing raw material by using the lactic acid-producing Escherichia coli.Type: GrantFiled: September 11, 2009Date of Patent: March 25, 2014Assignee: Mitsui Chemicals, Inc.Inventors: Takashi Morishige, Katsuyuki Takahashi, Hitoshi Takahashi, Mitsufumi Wada, Daisuke Mochizuki, Daisuke Miyazawa, Tadashi Araki
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Patent number: 8673600Abstract: Disclosed is an apparatus and a method for producing lactic acid, wherein only lactic acid is selectively absorbed and separated from fermentation liquor using a lactic acid absorption resin and wherein a neutralizing agent is not used. The present invention does not include a neutralizing process and a process of converting lactate to lactic acid. The method comprises: adding and mixing a culture medium, microorganism and sugar in a fermenter; passing a fermentation liquor through a microorganism filtration unit to remove microorganisms; and selectively absorbing and separating lactic acid from filtered liquid using a lactic acid absorption resin. The apparatus includes a fermenter for lactic acid fermentation; a filtration unit for removing a microorganisms from the fermentation liquor; and a lactic acid absorption resin for selectively absorbing lactic acid from the filtered liquid.Type: GrantFiled: December 18, 2012Date of Patent: March 18, 2014Assignee: Hyundai Motor CompanyInventors: Si Hwan Kim, Chae Hwan Hong, Jiyoun Seo, Do Suck Han
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Publication number: 20140075601Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: October 28, 2013Publication date: March 13, 2014Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
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Patent number: 8669093Abstract: A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector.Type: GrantFiled: September 17, 2004Date of Patent: March 11, 2014Assignee: Mitsui Chemicals, Inc.Inventors: Mitsufumi Wade, Toshihiro Oikawa, Daisuke Mochizuki, Junko Tokuda, Miyuki Kawashima, Tadashi Araki, Reiko Abe, Hitoki Miyake, Hitoshi Takahashi, Hideki Sawai, Takashi Mimitsuka, Takashi Morishige, Yosuke Higashi
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Patent number: 8663954Abstract: A method for the construction of a moderately thermophilic Bacillus strain capable of utilizing sucrose as a carbon source includes the transformation of a parent moderately thermophilic Bacillus strain not capable of utilizing sucrose as a carbon source with a polynucleotide comprising a DNA sequence that encodes a polypeptide having sucrose-specific phosphotransferase activity and having i) an amino acid sequence of SEQ ID NO:1 or ii) an amino acid sequence with an identity of at least 70% to the sequence of SEQ ID NO:1 and/or comprising a DNA sequence that encodes a polypeptide having sucrose-6-phosphate hydrolase activity and having iii) an amino acid sequence of SEQ ID NO:2 or iv) an amino acid sequence with an identity of at least 70% to the sequence of SEQ ID NO:2.Type: GrantFiled: July 15, 2010Date of Patent: March 4, 2014Assignee: Purac Biochem B.V.Inventors: Richard Van Kranenburg, Mariska Van Hartskamp
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Publication number: 20140051138Abstract: A lactic acid-producing yeast mutant which is a mutant of a yeast prepared by introduction of a lactate dehydrogenase gene that results in lactic acid-producing ability, the mutant having, in a medium at pH 3, a lactic acid-producing ability equivalent to or higher than the lactic acid-producing ability of a parent strain of the mutant at a lactic acid fermentation optimum pH.Type: ApplicationFiled: April 27, 2012Publication date: February 20, 2014Applicant: Toray Industries, Inc.Inventors: Kyungsu Na, Kazumi Suda