Abstract: To provide a method for producing an organic acid, whereby the desired organic acid can be efficiently recovered without necessity for adjusting the pH to a neutral level in the fermentation step. The method for producing an organic acid, comprises a first step of producing an organic acid by fermentation to obtain a crude liquid containing the organic acid and having a pH of from 1 to 5, and a second step of extracting the organic acid from the crude liquid containing the organic acid obtained in the first step by means of an extraction medium containing a C10-30 diester compound and an alkylamine compound to obtain an extract (1).
Abstract: A Corynebacterium including an NAD+ dependent formate dehydrogenase gene, and a method of producing C4 dicarboxylic acid using the Corynebacterium.
Type:
Application
Filed:
September 5, 2014
Publication date:
March 5, 2015
Inventors:
Soonchun CHUNG, Joonsong PARK, Jinhwan PARK, Jiae YUN, Jaechan PARK, Kwangmyung CHO
Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
Type:
Application
Filed:
March 17, 2014
Publication date:
February 26, 2015
Inventors:
Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D. LYNCH
Abstract: A genetically modified microorganism comprising a polynucleotide encoding ?-ketoglutarate synthase or a mutant thereof, and a polynucleotide encoding pyruvate carboxylase or a mutant thereof; wherein the genetically modified microorganism has decreased malate quinone oxidoreductase activity and/or decreased phosphoenolpyruvate carboxykinase activity compared to an unmodified microorganism of the same type, and wherein the genetically modified microorganism produces 4-hydroxybutyrate.
Type:
Application
Filed:
August 25, 2014
Publication date:
February 26, 2015
Inventors:
Youngmin LEE, Wooyong Lee, Jinwoo Kim, Jaechan Park, Jinhwan Park, Hwayoung Cho
Abstract: The present invention relates to a transformant which is transformed to express Baeyer-Villiger monooxygenase (BVMO), a method for producing C5-C14 medium-chain ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, or alcohols from C16-C20 long-chain fatty acids by biotransformation using the transformant, a method for producing a fatty acid derivative having an ester group which is introduced into the chain thereof from keto fatty acid using the BVMO, and novel ?-hydroxy fatty acids which are prepared by the method. Degradation products such as C5 to C14 ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, alcohols can be produced in a large amount from C16 to C20 long-chain fatty acids contained in a medium by biotransformation using a transformant capable of expressing BVMO of the present invention. Therefore, it can be widely used to produce ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids or alcohols in a more safe and economic manner.
Type:
Application
Filed:
April 5, 2013
Publication date:
February 26, 2015
Inventors:
Jin Byung Park, Ji Won Song, Eun Yeong Jeon
Abstract: The present invention relates to a process for recovering succinic acid in crystal form from a fermentation broth comprising succinic acid, comprising the steps of a) bringing the fermentation broth to a pH of between 1 and 4, b) crystallizing the succinic acid from the fermentation broth to form succinic acid crystals, c) dissolving the succinic acid crystals at a temperature of between 30 and 90 degrees Celsius to form an aqueous solution comprising dissolved succinic acid, d) crystallizing the succinic acid from the solution to recover succinic acid in crystal form. The invention further relates to succinic acid in crystal form, comprising a sugar content of 1 to 100 ppm and a nitrogen content of 1 to 80 ppm.
Type:
Application
Filed:
October 30, 2014
Publication date:
February 26, 2015
Inventors:
Maarten Job VAN DE GRAAF, Fredoen VALIANPOER, Guillaume FIEY, Loic DELATTRE, Elisabeth Agnes Michele SCHULTEN
Abstract: A method of sterilizing a separation membrane module using water vapor includes: a liquid supplying step of supplying a liquid having a boiling point of 80° C. or higher at atmospheric pressure to a secondary side of the separation membrane module such that a filling ratio of the liquid in a space surrounded by a filtration portion of a separation membrane is 70% or more, the filtration portion being used for filtration; a liquid sealing step of isolating the secondary side of the separation membrane module such that the filling ratio of the liquid supplied to the secondary side in the liquid supplying step is 70% or more; and a sterilization step of sterilizing the separation membrane module by supplying water vapor to a primary side of the separation membrane module while the secondary side of the separation membrane module is isolated.
Abstract: A recombinant Corynebacterium genus microorganism, and a method of producing C4 dicarboxylic acid under anaerobic conditions using the Corynebacterium genus microorganism.
Type:
Application
Filed:
August 11, 2014
Publication date:
February 12, 2015
Inventors:
Soonchun CHUNG, Joonsong Park, Jinhwan Park, Jiae Yun, Jaechan Park, Kwangmyung Cho
Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Application
Filed:
October 20, 2014
Publication date:
February 5, 2015
Inventors:
Suchindra Maiyuran, Randall Kramer, Paul Harris
Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Abstract: Embodiments of the present invention concerns methods and compositions for the construction of a series of vectors containing a chemical sensing module to assess the production of a chemical compound by a microorganism.
Type:
Application
Filed:
February 27, 2014
Publication date:
January 29, 2015
Applicant:
THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE
Inventors:
Ryan T. Gill, Michael D. Lynch, Tirzah Y. Mills
Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: A genetically engineered bacterial cell wherein activity of a pathway in the cell of converting ?-ketoglutarate into succinate semialdehyde; or activity of succinyl semialdehyde dehydrogenase in the cell is increased compared to the activity in a non-genetically engineered cell of the same type, and a method of producing succinic acid by using the same.
Type:
Application
Filed:
July 28, 2014
Publication date:
January 29, 2015
Inventors:
Joonsong PARK, Jinhwan Park, Soonchun Chung, Jiae Yun, Kwangmyung Cho
Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.
Type:
Application
Filed:
October 2, 2014
Publication date:
January 29, 2015
Inventors:
Hubertus Johannes Marie OP DEN CAMP, Harry Ramanoedj HARHANGI, Christiaan VAN DER DRIFT, Jacobus Thomas PRONK
Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.
Type:
Application
Filed:
October 9, 2014
Publication date:
January 22, 2015
Inventors:
Stephen Brown, Sheryl Luttringer, Debbie Yaver, Alan Berry
Abstract: Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described.
Type:
Application
Filed:
December 19, 2012
Publication date:
January 15, 2015
Inventors:
Zia Abdullah, Michael L. Dickens, Micah Paul McCreery, Randy L. Jones, Elvin Ray Beach, III, Jon-David S. Sears, Erin Suzanne Schultz, Stephanie Ann Smith, Paul E. George, II
Abstract: A method for degrading a readily degradable resin composition comprising an aliphatic polyester (A) which is biodegradable, and an aliphatic polyester (B?) which releases an acid upon hydrolysis and which is biodegradable at a higher degradation rate than that of the aliphatic polyester (A), the method comprising degrading the readily degradable resin composition in an enzyme reaction liquid containing a degradation enzyme, and an acid neutralizing agent incompatible with the enzyme reaction liquid.
Abstract: The invention relates to fungal strains having at least one genetic modification which leads to a reduction of the activity of at least one fungal carboxylic acid transporter and to a method for producing or using said fungal strains.
Type:
Application
Filed:
December 12, 2012
Publication date:
January 8, 2015
Applicant:
THYSSENKRUPP INDUSTRIAL SOLUTIONS AG
Inventors:
Andreas Aurich, Martina Holz, Anne Kretzschmar, Christina Otto, Gerold Barth, Isabel Waengler, Roland Arno Müller
Abstract: The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate.
Type:
Application
Filed:
July 11, 2014
Publication date:
January 8, 2015
Applicant:
Danisco US Inc.
Inventors:
Gopal K. CHOTANI, Manoj KUMAR, Jeff P. PUCCI, Karl J. SANFORD, Jayarama K. SHETTY
Abstract: The present invention provides methods for degrading or converting a cellulosic material using an enzyme composition in the presence of a reducing agent. The present invention also provides methods for producing a fermentation product and methods of fermenting a cellulosic material using an enzyme composition in the presence of a reducing agent.
Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Application
Filed:
September 15, 2014
Publication date:
January 1, 2015
Inventors:
Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Joergensen, Randall Kramer
Abstract: A microorganism expressing a vector encoding a CoA-dependent succinate semialdehyde dehydrogenase to efficiently produce a C4 compound, and methods for the use thereof.
Type:
Application
Filed:
June 30, 2014
Publication date:
January 1, 2015
Inventors:
Hong-soon RHEE, Young-min Lee, Jin-woo Kim, Eun-Young Park, Yeo-ju Song, Jin-hwan Park, Kwang-myung Cho
Abstract: The invention provides a microbial eukaryotic cell capable of utilizing C5 sugars, in particular xylose. Another objective of the invention is to provide an improved protein sequence to enable eukaryotic cells to degrade C5 sugars. The present invention thus provides protein comprising an amino acid sequence having at least 75% identity, preferably 80% identity, most preferably 90% identity, most highly preferably 95% identity to SEQ ID NO. 2 or SEQ ID NO. 8 and having xylose-isomerase activity in a eukaryotic cell.
Type:
Application
Filed:
February 7, 2013
Publication date:
December 25, 2014
Inventors:
Zdravko Dragovic, Christian Gamauf, Christoph Reisinger, Ulrich Kettling
Abstract: The present invention relates to a method for biologically treating carbon dioxide using the sulfur-oxidizing chemolithoautotroph Sulfurovum lithotrophicum 42BKT. The method of the present invention may enable carbon dioxide to be fixed or converted in high-concentration and high-pressure conditions which do not allow the biological photosynthetic conversion of microalgae or the like, and may exhibit high efficiency in the fixation of carbon dioxide as compared to existing methods for biologically treating carbon dioxide using microalgae. Further, the method of the present invention may use a gas mixture without a process of separating nitrogen and other gases, thus simplifying the process of the fixation or conversion of carbon diode.
Type:
Application
Filed:
September 20, 2012
Publication date:
December 25, 2014
Inventors:
Chang Ha Lee, Ik Sung Ahn, Hyuk Sung Kwon, Jae Hyuk Lee
Abstract: The present invention relates to microorganisms and polypeptides for detoxifying aldehydes associated with industrial fermentations. In particular, a heat-stable, NADPH- and iron-dependent alcohol dehydrogenase was cloned from Thermoanaerobacter pseudethanolicus 39E and displayed activity against a number of aldehydes including inhibitory compounds that are produced during the dilute-acid pretreatment process of lignocellulosic biomass before fermentation to biofuels. Methods to use the microorganisms and polypeptides of the invention for improved conversion of bio mass to biofuel are provided as well as use of the enzyme in metabolic engineering strategies for producing longer-chain alcohols from sugars using thermophilic, fermentative microorganisms.
Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, methods are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation.
Type:
Application
Filed:
September 11, 2014
Publication date:
December 25, 2014
Inventors:
Marshall Medoff, Thomas Craig Masterman, Seul-a Bae, Kelly Wallick
Abstract: The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
Type:
Application
Filed:
December 18, 2012
Publication date:
December 18, 2014
Inventors:
Ee Lui Ang, Ellen D. Eberhard, Xiyun Zhang, Wei Zhang, Jing Tian, Derek J. Smith, Vesna Mitchell
Abstract: Provided herein are genetically modified yeast cells for the production of succinate, methods of making these yeast cells, and methods of using these cells to produce succinate.
Abstract: Processes are described for fractionating lignocellulosic biomass into cellulose, hemicellulose, and lignin, comprising fractionating lignocellulosic biomass in the presence of a solvent for lignin (such as ethanol), a hydrolysis catalyst (such as sulfur dioxide), and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin; hydrolyzing the hemicellulose to produce hemicellulosic monomers; saccharifying the cellulose-rich solids to produce glucose; recovering the hemicellulosic monomers and the glucose, separately or in a combined stream, as fermentable sugars; and fermenting the fermentable sugars to a fermentation product having a higher normal boiling point than water. Process integration of mass and/or energy is disclosed in many specific embodiments. The fermentation product may include an organic acid, an alcohol, a diol, or combinations thereof.
Type:
Application
Filed:
May 23, 2014
Publication date:
December 4, 2014
Applicant:
API Intellectual Property Holdings, LLC
Inventors:
Theodora RETSINA, Vesa PYLKKANEN, Ryan P. O'CONNOR
Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
Type:
Application
Filed:
July 18, 2014
Publication date:
November 27, 2014
Inventors:
MICHAEL DAUNER, Lori Ann Maggio-Hall, Jean-Francois Tomb
Abstract: This invention belongs to the field of biochemical engineering and relates to a method of cyclic utilization of water during separation of succinic acid made by fermentation. This invention uses water from separation process for aerobic growth of E.coli AFP111 and production of succinic acid by anaerobic fermentation, obtaining final succinic acid concentration of 55 g/L and yield of 91.6%. Compared with results of fermentation using culture medium prepared from tap water, succinic acid concentration and productivity increased by 8.5% and 8.46%, respectively. An outstanding advantage of this invention is recovery and utilization of evaporated water during separation of succinic acid, realizing cyclic use of water during industrial production of succinic acid, which is an environment-friendly process.
Type:
Application
Filed:
September 13, 2012
Publication date:
November 27, 2014
Applicant:
NANJING UNIVERSITY OF TECHNOLOGY
Inventors:
Min Jiang, Min Zhang, Jiangfeng Ma, Hao Wu, Liya Liang, Rongming Liu, Guangming Wang, Ping Wei
Abstract: The present invention relates to a process for producing a dicarboxylic acid comprising fermenting a fungal cell in a vessel comprising a suitable fermentation medium, comprising adding a gas flow which comprises 20 to 35 v/v % of oxygen and less than 0.1v/v % of carbon dioxide to the fermentation medium, and maintaining an average partial carbon dioxide pressure of at least about 0.35 bar in the fermentation medium, and producing the dicarboxylic acid.
Type:
Application
Filed:
July 2, 2012
Publication date:
November 20, 2014
Applicant:
DSM II Assets B.V.
Inventors:
Mickel Leonardus August Jansen, Joseph Johannes Heijnen, Rene Verwaal
Abstract: Provided are isolated polypeptides having xylanase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
Type:
Application
Filed:
November 22, 2012
Publication date:
November 20, 2014
Inventors:
Yu Zhang, Lan Tang, Junxin Duan, Ye Liu
Abstract: The present invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having formate dehydrogenase activity. The present invention also relates to a method of manufacturing succinic acid and the use of the bacterial cell for the manufacture of succinic acid.
Type:
Grant
Filed:
December 21, 2009
Date of Patent:
November 11, 2014
Assignee:
BASF SE
Inventors:
Edzard Scholten, Stefan Haefner, Hartwig Schröder
Abstract: Provided are isolated polypeptides having beta-glucosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Application
Filed:
December 19, 2012
Publication date:
November 6, 2014
Inventors:
Ye Liu, Junxin Duan, Yu Zhang, Lan Tang
Abstract: The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
Type:
Grant
Filed:
January 15, 2008
Date of Patent:
November 4, 2014
Assignee:
Korea Advanced Institute of Science and Technology
Inventors:
Sang Yup Lee, Ji Mahn Kim, Jeong Wook Lee, Hyohak Song, Sol Choi
Abstract: The present invention is concerned with bacteria for the production of succinic acid. Specifically, the invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having isocitrate lyase activity and a heterologous polypeptide having malate synthase activity. Further, the present invention contemplates a polynucleotide comprising a nucleic acid encoding a polypeptide having isocitrate lyase activity and a nucleic acid encoding a polypeptide having malate synthase activity. Finally, the present invention relates to the use of a bacterial cell of the invention for the manufacture of succinic acid.
Type:
Grant
Filed:
December 22, 2009
Date of Patent:
November 4, 2014
Assignee:
BASF SE
Inventors:
Edzard Scholten, Stefan Haefner, Hartwig Schröder
Abstract: A process for production of C5 and C6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a C1-C2 acid (e.g., acetic acid) with washing thereof with a C1-C2 acid miscible organic solvent, (e.g., ethyl acetate). A soluble hemicellulose and lignin enriched fraction is obtained separately from a cellulose pulp enriched fraction and lignin is removed from the soluble hemicellulose fraction. These fractions contain acylated (e.g., acetylated) cellulose and hemicellulose, which are deacylated by treatment with an alkali and/or with an acetyl esterase enzyme. The deacylated fractions are then digested with suitable cellulolytic and/or hemicellulolytic enzymes, preferably in the presence of non-ionic detergent to yield the C5 and C6 enriched syrups.
Type:
Application
Filed:
May 16, 2014
Publication date:
October 30, 2014
Applicant:
ARCHER DANIELS MIDLAND COMPANY
Inventors:
Wuli Bao, Thomas Binder, Charles Abbas, Lucas Loveless
Abstract: This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.
Type:
Grant
Filed:
November 17, 2010
Date of Patent:
October 28, 2014
Assignee:
Myriant Corporation
Inventors:
Tammy Grabar, Wei Gong, R. Rogers Yocum
Abstract: A method is provided for improving enzymatic hydrolysis in saccharification of a lignocellulosic material. The method is comprising pretreating the lignocellulosic material to obtain a slurry of pretreated lignocellulosic material; adding at least one reducing agent to the slurry of pretreated lignocellulosic material or the liquid fraction thereof to decrease the enzymatic hydrolysis inhibitory properties of slurry of the pretreated lignocellulosic material or the liquid fraction thereof; and subjecting the slurry of pretreated lignocellulosic material or the liquid fraction thereof to enzymatic hydrolysis in the presence of the at least one reducing agent.
Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase, a thermostable protease, and optionally a carbohydrate-source generating enzyme and/or pullulanase, are present and/or added during liquefaction. The invention also relates to compositions suitable for use in a process of the invention.
Type:
Application
Filed:
November 30, 2012
Publication date:
October 23, 2014
Inventors:
Randall Deinhammer, Joyce Craig, Tomoko Matsui, Shinobu Takagi, Suzanne Clark, John Matthews, Anne Glud Hjulmand, Chee-Leong Soong
Abstract: The present application provide methods for producing ethanol from a biomass. The methods combine sugars produced from a feedstock containing starch with sugars produced from a cellulosic biomass. The methods allow increased amounts of ethanol to be produced from a given solids concentration in the fermenters. The methods also encompass filtering the liquefied feedstock mash through a filter comprising biomass fibers. The biomass filter produces a post-filtered mash stream comprising a high concentration of sugars and a low concentration of non-fermentable solids. The methods provide numerous advantages described herein.
Abstract: A process for the preparation of a monovalent succinate salt includes: a) fermenting a carbohydrate source to succinic acid by means of a micro-organism, b) adding a alkaline earth metal hydroxide, carbonate and/or hydrogencarbonate, the alkaline earth metal being calcium or magnesium, as neutralising agent during the fermentation in an aqueous medium and causing the formation of calcium succinate or magnesium succinate, c) reacting the alkaline earth metal succinate salt in an aqueous medium with a monovalent hydroxide, carbonate and/or hydrogencarbonate base to form an alkaline earth metal hydroxide, carbonate and/or hydrogencarbonate and a monovalent succinate salt, d) separating the monovalent succinate salt from the alkaline earth metal hydroxide, carbonate and/or hydrogencarbonate, and e) recycling the alkaline earth metal hydroxide, carbonate and/or hydrogencarbonate to step b.
Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.
Abstract: The invention refers to a method of biotransforming a carbohydrate of a raw material into a chemical, by cultivating Lactobacillus diolivorans in the presence of the raw material to produce a chemical substance, and isolating the chemical substance in the purified form, and the use of L. diolivorans in one of a series of biotransformation methods, wherein carbohydrates from at least two different carbohydrate sources of low purity are transformed into chemicals.
Type:
Application
Filed:
November 5, 2012
Publication date:
October 16, 2014
Inventors:
Michael Sauer, Hans Marx, Stefan Pfluegl, Diethard Mattanovich
Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.