Abstract: Methods and compositions are provided for improving the production of products, such as fuel products like ethanol, in microorganisms. In particular, methods and compositions are described for improving ethanol production utilizing genes identified in Clostridium phytofermentans.
Type:
Application
Filed:
April 14, 2011
Publication date:
August 2, 2012
Applicants:
Qteros, Inc., University of Massachusetts
Inventors:
Jeffrey Blanchard, Susan Leschine, Elsa Petit, John Fabel, Matthias Schmalisch
Abstract: The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes.
Type:
Application
Filed:
August 29, 2011
Publication date:
August 2, 2012
Inventors:
Ranjini Chatterjee, Kenneth Zahn, Kenneth Mitchell, Yaoquan Liu
Abstract: The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes.
Type:
Application
Filed:
August 29, 2011
Publication date:
August 2, 2012
Inventors:
RANJINI CHATTERJEE, KENNETH ZAHN, KENNETH MITCHELL, YAOQUAN LIU
Abstract: This invention provides microbial organisms, particularly yeasts such as Yarrowia lipolytica, that have one or more disrupted genes. The gene disruption(s) may yield improved production of fatty acyl-CoA derivatives.
Type:
Application
Filed:
December 19, 2011
Publication date:
June 28, 2012
Applicant:
Codexis, Inc.
Inventors:
Douglas A. Hattendorf, Jennifer L. Shock, Louis Clark
Abstract: A process for preparing optically active saturated aldehydes or alcohols of the formula (2) from ?,?-unsaturated aldehydes of the formula (1) by reduction in the presence of an enoate reductase (i) having the polypeptide sequence SEQ ID No. 1 or 2, or (ii) having a polypeptide sequence which is at least 80% identical to the sequence of SEQ ID No. 1 or 2.
Type:
Grant
Filed:
December 19, 2006
Date of Patent:
June 26, 2012
Assignee:
BASF SE
Inventors:
Andreas Schädler, Thomas Friedrich, Rainer Stürmer, Sabine Rinck
Abstract: A method of converting hydroxymethylfurfural and is derivative species into hydroxymethylfurfural oxidation products is disclosed. The method includes contacting the hydroxymethylfurfural species in a mixture with an enzyme that oxidizes the hydroxymethylfurfural species while controlling hydrogen peroxide in the mixture. In one exemplary embodiment the enzyme is chloroperoxidase and the hydrogen peroxide is metered into the mixture to predominantly and selectively make at least one of formylfuran carboxylic acid or furan dicarboxylic acid. In another embodiment the enzyme is aryl alcohol oxidase and catalase is included in the mixture to remove unwanted hydrogen peroxide by product and the reaction predominantly makes at least one of dimethylfuran or formylfuran carboxylic acid. When the predominant product is a carboxylic acid or furan dicarboxylic acid, it can be recovered in substantially pure form by acid precipitation.
Abstract: The invention provides consolidated bioprocessing methods and host cells. The host cells are capable of directly converting biomass polymers or sunlight into biodiesel equivalents and other fatty acid derivatives. In particular, the invention provides a method for producing biodiesel equivalents and other fatty acid derivatives from a biomass polymer including providing a genetically engineered host cell, culturing the host cell in a medium containing a carbon source such that recombinant nucleic acids in the cell are expressed, and extracting biodiesel equivalents and other fatty acid derivatives from the culture.
Type:
Application
Filed:
April 30, 2010
Publication date:
May 10, 2012
Applicant:
The Regents of the University of California
Inventors:
Jay D. Keasling, Yisheng (Connie) Kang, Eric J. Steen, Gregory Bokinsky
Abstract: A process for the enzymatic reduction of an enoate (1) wherein the C?C bond of the enoate (1) is stereoselectively hydrogenated in the presence of an enoate-reductase and an oxidizable co-substrate (2) in a system which is free of NAD(P)H, a. b. in which c. A is a ketone radical (—CRO), an aldehyde radical (—CHO), a carboxyl radical (—COOR), with R?H or optionally substituted C1-C6-alkyl radical, d. R1, R2 and R3 are independently of one another H, —O-C1-C6-alkyl , —O—W with W=a hydroxyl protecting group, C1-C6-alkyl, which can be substituted, C2-C6-alkenyl, carboxyl, or an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or one of R1, R2 and R3 is a —OH radical, or R1 is linked to R3 so as to become part of a 4-8-membered cycle, or R1 is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R1, R2 and R3 may not be identical.
Type:
Application
Filed:
May 31, 2010
Publication date:
March 22, 2012
Applicant:
BASF SE
Inventors:
Steffen Maurer, Bemhard Hauer, Melanie Bonnekesse, Kurt Faber, Clemens Stückler
Abstract: The invention relates to a process for the production of optically active Citronellal by enzymatic reduction of Citral with a reductase from Zymomonas mobilis.
Type:
Grant
Filed:
November 10, 2006
Date of Patent:
February 28, 2012
Assignee:
BASF SE
Inventors:
Rainer Stuermer, Thomas Friedrich, Andre Mueller, Bernhard Hauer, Bettina Rosche
Abstract: The invention relates to methods for producing aldehydes and the oxidation and reduction products thereof.
Type:
Application
Filed:
April 28, 2010
Publication date:
February 9, 2012
Applicant:
EVONIK DEGUSSA GmbH
Inventors:
Thomas Haas, Florian Klasovsky, Hendrik Krauter, Steffen Schaffer, Rene Schoebel, Thomas Tacke, Klaus-Dieter Vorlop, Thomas Willke, Mirja Wessel
Abstract: The invention provides methods and compositions for the production of L-ribitol and other rare sugars using a mannitol-1-dehydrogenase or a polyol-1-dehydrogenase.
Abstract: A method of producing a low molecular weight organic compound (e.g. a plant or bacteria secondary metabolite) in increased yields involving use of a microorganism cell, which comprises a gene involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound and a glycosyltransferase gene capable of glycosylating the produced aglycon.
Type:
Application
Filed:
August 8, 2011
Publication date:
December 29, 2011
Applicant:
EVOLVA SA
Inventors:
Joergen Hansen, Thomas Hvid Andersen, Finn Thyge Okkels
Abstract: An exclusive manufacturing technique for extracting active interface saponin and organic substances from soapberry; organic elements and oleic alcohol products from soapberry seeds through the process of fermentation and end products made therefrom, wherein the manufacturing process includes: 1. Pre-ferment soapberry fruit (1). 2. Processing said fruit (1) by a dialysis device (2). 3. Separating the soapberry flesh (12) and fiber (13) by a separation device (3). 4. Extracting soapberry dialytic liquid (A) through a grinding and compression device (4) and separating the fiber (13). 5. Eliminating bacteria inside the dialytic liquid (A) by a huge stewing device (5). 6. A second fermenting process by a vacuum device (6) and generating a soapberry syrup (D). Said method is healthy, toxin free and biologically safe, produce no wastage, zero carbon emissions, zero pollution, low energy production and is ecologically friendly. End products produced by said method are variables with excellent economic viability.
Abstract: The invention relates to a compound according to Formula (IX) and salts thereof, wherein R1, R2 and R5 are each independently selected from H and hydrocarbon moieties, which hydrocarbon moieties optionally comprise one or more heteroatoms, and which hydrocarbons optionally comprise substituents, or when the compound according to formula (IX) is a salt, R1 and/or R2 may be a cation, R3, and R4 each independently selected from hydrocarbon moieties, which hydrocarbon moieties optionally comprise one or more heteroatoms, and which hydrocarbons optionally comprise substituents, and wherein any two of R1, R2, R3, R4 and R5 are optionally linked together to form a ring structure. The invention further relates to the preparation of such a compound and to the use of such a compound for preparing a pharmaceutical compound, an agrochemical compound, an intermediate for a pharmaceutical compound or an intermediate for an agrochemical compound.
Type:
Application
Filed:
August 8, 2011
Publication date:
December 1, 2011
Applicant:
DSM IP Assets B.V.
Inventors:
Gerardus Karel Maria Verzijl, Henricus Martinus Maria Gerardus Straatman, Andreas Hendrikus Maria De Vries, Lizette Schmieder, Jeroen Antonius Franciscus Boogers
Abstract: A process is provided to produce a concentrated aqueous peracid solution in situ using at least one enzyme having perhydrolase activity in the presence of hydrogen peroxide (at a concentration of at least 500 mM) under neutral to acidic reaction conditions from suitable carboxylic acid esters (including glycerides) and/or amides substrates. The concentrated peracid solution produced is sufficient for use in a variety of disinfection and/or bleaching applications.
Type:
Grant
Filed:
May 22, 2009
Date of Patent:
November 22, 2011
Assignee:
E. I. du Pont de Nemours and Company
Inventors:
Robert DiCosimo, Mark Scott Payne, Eugenia Costa Hann, Vincent Brian Croud, John Edward Gavagan, Lorraine Winona Wagner
Abstract: The invention provides recombinant microorganisms capable of producing isobutyraldehyde using CO2 as a carbon source. The invention further provides methods of preparing and using such microorganisms to produce isobutyraldehyde.
Type:
Application
Filed:
June 20, 2011
Publication date:
October 13, 2011
Applicant:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Abstract: The present invention relates to a transformed microorganism capable of (a) a higher xylose isomerase activity than the equivalent microorganism prior to transformation; and/or (b) a higher growth rate in or on a growth medium comprising xylose than the equivalent microorganism prior to transformation; and/or (c) a faster metabolism of xylose than the equivalent microorganism prior to transformation; and/or (d) a higher production of ethanol when grown anaerobically on xylose as the carbon source than the equivalent microorganism prior to transformation.
Type:
Application
Filed:
December 10, 2009
Publication date:
October 6, 2011
Applicant:
TERRANOL A/S
Inventors:
Birgitte Rönnow, Thomas Hvid Andersen, Ole Sibbesen
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Application
Filed:
March 23, 2011
Publication date:
September 29, 2011
Inventors:
ROBERT DICOSIMO, John Edward Gavagan, Mark Scott Payne
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Application
Filed:
March 23, 2011
Publication date:
September 29, 2011
Inventors:
Robert Dicosimo, John Edward Gavagan, Mark Scott Payne
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Application
Filed:
March 23, 2011
Publication date:
September 29, 2011
Inventors:
Robert Dicosimo, John Edward Gavagan, Mark Scott Payne
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Application
Filed:
March 23, 2011
Publication date:
September 29, 2011
Inventors:
Robert Dicosimo, John Edward Gavagan, Mark Scott Payne
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Application
Filed:
March 23, 2011
Publication date:
September 29, 2011
Inventors:
ROBERT DICOSIMO, John Edward Gavagan, Mark Scott Payne
Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.
Type:
Application
Filed:
May 17, 2011
Publication date:
September 22, 2011
Applicant:
Genomatica, Inc.
Inventors:
Robert Haselbeck, John D. Trawick, Wei Niu, Anthony P. Burgard
Abstract: A process of producing an organic compound and/or an intermediary compound as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting the culture to light, wherein the cell is capable of expressing a nucleic acid molecule, wherein the expression of the nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into the organic compound and/or into the intermediary compound and wherein the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in the culture.
Type:
Application
Filed:
December 17, 2008
Publication date:
September 1, 2011
Applicant:
UNIVERSITEIT VAN AMSTERDAM
Inventors:
Klaas Jan Hellingwerf, Maarten Joost Teixeira De Mattos
Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.
Abstract: A bioengineered synthesis scheme for the production of L-1,2,4-butanetriol, D-1,2,4-butanetriol and racemic mixtures thereof from a carbon source is provided. Methods of producing L-1,2,4-butanetriol, D-1,2,4-butanetriol and racemic mixtures thereof are also provided. Methods are also provided for converting D-1,2,4-butanetriol and L-1,2,4,-butanetriol to D,L-1,2,4-butanetriol trinitrate.
Type:
Application
Filed:
March 2, 2011
Publication date:
July 7, 2011
Applicant:
The Board of Trustees of Michigan State University
Abstract: An isolated nucleic acid molecule cloned from Artemisia annua encodes an alcohol dehydrogenase (Adh1). Artemisia annua Adh1 enzymatically oxidizes artemisinic alcohol to artemisinic aldehyde. The nucleic acid molecule, and the enzyme encoded thereby, may be used in processes to produce artemisinic aldehyde, dihydroartemsinic aldehyde, artemisinic acid and/or dihydroartemisinic acid in a host cell. Artemisinic aldehyde, dihydroartemisinic aldehyde, artemisinic acid and/or dihydroartemisinic acid can be chemically converted to the antimalarial compound artemisinin.
Type:
Application
Filed:
July 13, 2009
Publication date:
June 30, 2011
Inventors:
Devin Polichuk, Keat (Thomas) H. Teoh, Yansheng Zhang, Kenneth W. Ellens, Darwin W. Reed, Patrick S. Covello
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Application
Filed:
February 28, 2011
Publication date:
June 23, 2011
Inventors:
ROBERT DICOSIMO, John Edward Gavagan, Mark S. Payne
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Application
Filed:
February 28, 2011
Publication date:
June 23, 2011
Inventors:
ROBERT DICOSIMO, John Edward Gavagan, Mark S. Payne
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Application
Filed:
February 28, 2011
Publication date:
June 23, 2011
Inventors:
ROBERT DICOSIMO, John Edward Gavagan, Mark S. Payne
Abstract: A method of producing a low molecular weight organic compound (e.g. a plant or bacteria secondary metabolite) in increased yields involving use of a microorganism cell, which comprises a gene involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound and a glycosyltransferase gene capable of glycosylating the produced aglycon.
Type:
Application
Filed:
October 21, 2010
Publication date:
June 9, 2011
Applicant:
BLMAPS
Inventors:
Joergen Hansen, Thomas Hvid Andersen, Finn Thyge Okkels
Abstract: This invention relates to microorganism cells that are modified to increase conversion of carbon dioxide and/or carbon monoxide to a product, such as a fatty acid methyl ester, and to related methods and systems. A pathway from the Calvin Benson Cycle to the product is provided, which in various embodiments involves use of heterologous proteins that exhibit desired enzymatic conversions.
Abstract: A unique, innovative, and efficient system in order to convert ferulic acid into vanillin which involves the immobilization of actinomycetes in an effective solid support structure which is comprised of one or more pieces of a material, that is configured so as to allow effective aeration. For the immobilization of actinomycetes a surface culture is employed in which these microorganisms develop a mycelial growth pattern and adhere to the solid support structure. The use of surface culture also allows us to benefit from enhanced physiological responses such as increased growth rates and production yields of the actinomycetes when they are cultivated in this surface culture. Since the microorganisms are held captive in the supports, the step of biocatalysis can be repeated up to 15 times before the biocatalytic capacity is lost. An evaluation of the whole transformation process results in a higher vanillin yield.
Type:
Application
Filed:
October 18, 2008
Publication date:
March 17, 2011
Applicant:
BIOKAB, S.A. de C.V.
Inventors:
Ali Asaff Torres, Mayra De La Torre Martinez, Antonino Berrondo Mir, Roberto Miguel Macias Ochoa
Abstract: A process is described for producing fermentable sugars derivable from biomass that contains polysaccharide, such as cellulose, made increasingly accessible as a substrate for enzymatic degradation or other methods of depolymerization. These fermentable sugars are subsequently able to be fermented to produce various target chemicals, such as alcohols, aldehydes, ketones or acids.
Type:
Application
Filed:
February 3, 2010
Publication date:
February 17, 2011
Applicant:
HERCULES INCORPORATED
Inventors:
Herbert T. CONNER, Patrick J. COWAN, John C. GAST
Abstract: The invention provides a nucleic acid molecule comprising: (a) a nucleotide sequence as shown in SEQ ID No. 35; or (b) a nucleotide sequence which is the complement of SEQ ID No. 35; or (c) a nucleotide sequence which is degenerate with SEQ ID No. 35; or (d) a nucleotide sequence hybridising under conditions of high stringency to SEQ ID No. 35, to the complement of SEQ ID No. 35, or to a hybridisation probe derived from SEQ ID No. 35 or the complement thereof; or (e) a nucleotide sequence having at least 80% sequence identity with SEQ ID No. 35; or (f) a nucleotide sequence having at least 65% sequence identity with SEQ ID No. 35 wherein said sequence preferably encodes or is complementary to a sequence encoding a nystatin PKS enzyme or a part thereof.
Abstract: The present disclosure provides a process for the production of long chain fatty alcohols by recombinant host cells expressing one or more heterologous carboxylic acid reductase enzymes useful for the conversion of fatty acids, and derivatives thereof, to long chain fatty alcohols.
Type:
Application
Filed:
May 21, 2010
Publication date:
November 25, 2010
Applicant:
CODEXIS, INC.
Inventors:
Behnaz Behrouzian, Robert McDaniel, Xiyun Zhang, Louis Clark
Abstract: An isolated nucleic acid molecule cloned from Artemisia annua encodes an artemisinic aldehyde double bond reductase. Artemisinic aldehyde double bond reductase enzymatically reduces artemisinic aldehyde to (11R)-dihydroartemisinic aldehyde. The nucleic acid molecule, and the enzyme encoded thereby, may be used in processes to produce dihydroartemsinic aldehyde and/or dihydroartemisinic acid in a host cell. Dihydroartemisinic acid is a late precursor to the antimalarial compound artemisinin.
Type:
Application
Filed:
November 19, 2008
Publication date:
November 25, 2010
Inventors:
Yansheng Zhang, Thomas Keat Teoh, Darwin W. Reed, Douglas J.H. Olson, Andrew R. S. Ross, Patrick S. Covello
Abstract: The invention relates to a method for producing sugars, such as glucose, by fractionating lignocellulose-containing biomass. The sugar product thus obtained is useful for the manufacture of bioethanol and other chemicals.
Type:
Application
Filed:
November 7, 2008
Publication date:
September 23, 2010
Inventors:
Juha Anttila, Juha Tanskanen, Pasi Rousu, Päivi Rousu, Keijo Hytönen
Abstract: Compositions and methods for producing hydrocarbons such as aldehydes, alkanes, and alkenes are described herein. Certain hydrocarbons can be used in biofuels.
Type:
Application
Filed:
February 22, 2010
Publication date:
September 2, 2010
Applicant:
LS9, INC.
Inventors:
ANDREAS SCHIRMER, MATHEW RUDE, SHANE BRUBAKER
Abstract: Fatty acid 13-hydroperoxide lyase proteins which have been modified with respect to a previously described guava 13-hydroperoxide lyase and the nucleic acid sequences encoding these proteins. Also, recombinant nucleic acid molecules for expressing the modified 13-hydroperoxide lyases and methods of using such lyases in the field of organic synthesis.
Abstract: The present invention relates to polynucleotides from Helobdella robusta, Laccaria bicolor, Lottia gigantea, Microcoleus chthonoplastes, Monosiga brevicollis, Mycosphaerella fijiensis, Mycospaerella graminicola, Naegleria gruberi, Nectria haematococca, Nematostella vectensis, Phycomyces blakesleeanus, Trichoderma resii, Physcomitrella patens, Postia placenta, Selaginella moellendorffii and Microdochium nivale, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention.
Abstract: The invention relates to a compound according to Formula (IX) and salts thereof, wherein R1, R2 and R5 are each independently selected from H and hydrocarbon moieties, which hydrocarbon moieties optionally comprise one or more heteroatoms, and which hydrocarbons optionally comprise substituents, or when the compound according to formula (IX) is a salt, R1 and/or R2 may be a cation, R3, and R4 each independently selected from hydrocarbon moieties, which hydrocarbon moieties optionally comprise one or more heteroatoms, and which hydrocarbons optionally comprise substituents, and wherein any two of R1, R2, R3, R4 and R5 are optionally linked together to form a ring structure. The invention further relates to the preparation of such a compound and to the use of such a compound for preparing a pharmaceutical compound, an agrochemical compound, an intermediate for a pharmaceutical compound or an intermediate for an agrochemical compound.
Type:
Application
Filed:
July 11, 2008
Publication date:
August 5, 2010
Inventors:
Gerardus Karel Maria Verzijl, Henricus Martinus Maria Gerardus Straatman, Andreas Hendrikus Maria De Vries, Lizette Schmieder, Jeroen Antonius Franciscus Boogers
Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Type:
Application
Filed:
December 23, 2009
Publication date:
July 29, 2010
Applicant:
CODEXIS, INC.
Inventors:
Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
Abstract: Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting suitable monosaccharides or oligosaccharides, such as those derived from biomass, as well as various aldehydes and/or ketones, into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same.
Type:
Application
Filed:
December 11, 2009
Publication date:
July 22, 2010
Inventors:
Yasuo Yoshikuni, Adam J. Wargacki, Asael Herman
Abstract: A process for the production of natural ferulic acid, coniferyl alcohol and/or vanillin, includes the bio-conversion of eugenol by a bacteria belonging to the Streptomyces genes including at least one nucleotide sequence SEQ ID NO:1 or SEQ ID NO:8 or any nucleotide sequence having at least 70%, preferably 80% and very preferably 90%, identity with the sequence SEQ ID NO:1 or SEQ ID NO:8.
Abstract: The invention relates to a method for the oxidative cleavage of vinyl aromatics of the formula (1) characterized in that (a) compound(s) of the formula (1) is/are oxidized to aldehydes and ketones of the formulas (2) and (3), respectively, in the presence of molecular oxygen using at least one enzyme selected from peroxidases and laccases as a catalyst, according to the following general reaction scheme: wherein n is an integer of 0 to 5; the R1 are selected from saturated or unsaturated hydrocarbon groups with 1 to 10 carbon atoms, wherein carbon atoms are optionally substituted by heteroatoms and are optionally further substituted, amino, C1-6 alkylamino and C1-6 dialkylamino groups, halogens, hydroxy and cyano, wherein two of the substituents R1 may be linked to form a ring; R2 and R3 are each independently hydrogen or one of the options for R1, wherein R2 and/or R3 may be linked with R1 to form a ring, in which case R2 and R3 may each represent a chemical bond.
Abstract: The present invention provides for a method of producing one or more fatty acid derived compounds in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived compounds. The invention provides for the biosynthesis of fatty acid derived compounds such as C18 aldehydes, C18 alcohols, C18 alkanes, and C17 alkanes from C18-CoA which in turn is synthesized from butyryl-CoA. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism.
Type:
Application
Filed:
December 21, 2009
Publication date:
July 8, 2010
Applicant:
The Regents of the University of California