Butanol Patents (Class 435/160)
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Publication number: 20140342420Abstract: The present invention provides a method for the production of an alkanol from biomass. The method for producing an alkanol, including n-butanol, comprises fermenting a carbohydrate to produce a diacid and converting the diacid into an alkanol by solution chemistry or solid phase chemistry. The carbohydrate may be derived or extracted from a lignocellulose, an algae, a grain, or a starch containing root.Type: ApplicationFiled: August 22, 2012Publication date: November 20, 2014Applicant: SUGANIT SYSTEMS, INC.Inventors: Guneet Kumar, Praveen Paripati
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Patent number: 8889385Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.Type: GrantFiled: March 15, 2013Date of Patent: November 18, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
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Publication number: 20140335582Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.Type: ApplicationFiled: July 23, 2014Publication date: November 13, 2014Inventors: Gail K. DONALDSON, Andrew C. Eliot, Dennis Flint, Michael Charles Grady, Mehmedalija Jahic, Lori Ann Maggio-Hall, Vasantha Nagarajan, Ranjan Patnaik
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Publication number: 20140335583Abstract: Methods and systems for producing a biofuel using genetically modified iron-oxidizing bacteria (IOB) are disclosed. In some embodiments, the methods include the following: providing an IOB that have been genetically modified to enable them to generate a biofuel or chemical; feeding a first source of ferrous iron to the IOB; feeding water, carbon dioxide, and oxygen to the IOB; producing at least the biofuel or chemical, ferric iron, and an IOB biomass; and preventing ferric precipitates from forming. In some embodiments, the methods and systems include the following: a bioreactor including IOB that have been genetically modified to enable them to generate a biofuel; a first source of ferrous iron; sources of water, carbon dioxide, and oxygen; a source of anti-ferric precipitating agent in fluid communication with the bioreactor; and a electrochemical reactor that is configured to electrochemically reduce ferric iron to a second source of ferrous iron.Type: ApplicationFiled: July 28, 2014Publication date: November 13, 2014Inventors: Alan West, Scott Banta
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Publication number: 20140331364Abstract: Provided are isolated polypeptides having beta-glucosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 19, 2012Publication date: November 6, 2014Inventors: Ye Liu, Junxin Duan, Yu Zhang, Lan Tang
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Publication number: 20140329285Abstract: A method for on-farm processing a biomass feedstock into a useful industrial chemicals includes the steps of (a) delignifying the biomass feedstock to produce a delignified biomass, (b) subjecting the delignified biomass to cellulase production, (c) subjecting the delignified biomass with attached cellulase to simultaneous cellulolytic and solventogenic reactions to produce useful industrial chemicals (d) collecting and separating the useful industrial chemical from the fermentation broth and (e) collecting the fermentation residues.Type: ApplicationFiled: October 25, 2013Publication date: November 6, 2014Applicant: The University of Kentucky Research FoundationInventors: Sue E. Nokes, Bert C. Lynn, Stephen Rankin, Barbara Knutson, Michael D. Montross, Michael Flythe
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Patent number: 8877472Abstract: Corn or soy plant biomass is electron beam irradiation processed and saccharified to produce sugars. The sugars are then converted to products such as alcohols, organic acids, hydrocarbons, hydrogen, proteins, carbohydrates, fats, oils, lipids, amino acids, vitamins, and mixtures thereof.Type: GrantFiled: May 24, 2013Date of Patent: November 4, 2014Assignee: Xyleco, Inc.Inventor: Marshall Medoff
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Publication number: 20140322774Abstract: Microorganisms and methods of producing n-butyraldehyde with enhanced yields are presented in which a microorganism is engineered to enhance the conversion of a carbon source into n-butyraldehyde. The n-butyraldehyde is recovered by way of a gas stripping process that occurs during the conversion process, providing significantly greater product yield than post-fermentation recovery of n-butyraldehyde alone.Type: ApplicationFiled: November 2, 2012Publication date: October 30, 2014Inventors: Kwang Myung Cho, Wendy Higashide, Chrissie Lee, Shahrooz Rabizadeh
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Publication number: 20140322780Abstract: This invention describes genes, metabolic pathways, microbial strains and methods to produce methylbutanol and other compounds of interest from renewable feedstocks.Type: ApplicationFiled: November 28, 2011Publication date: October 30, 2014Inventors: Stephen Picataggio, Robert C. Brown, Jessica R. Kristof, Gena Roy, Prachee Prakash, Stuart A. Underwood, Kevin Watts, Kevin V. Martin
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Patent number: 8871475Abstract: A method is provided for improving enzymatic hydrolysis in saccharification of a lignocellulosic material. The method is comprising pretreating the lignocellulosic material to obtain a slurry of pretreated lignocellulosic material; adding at least one reducing agent to the slurry of pretreated lignocellulosic material or the liquid fraction thereof to decrease the enzymatic hydrolysis inhibitory properties of slurry of the pretreated lignocellulosic material or the liquid fraction thereof; and subjecting the slurry of pretreated lignocellulosic material or the liquid fraction thereof to enzymatic hydrolysis in the presence of the at least one reducing agent.Type: GrantFiled: October 6, 2010Date of Patent: October 28, 2014Assignee: Sekab E-Technology ABInventors: Bjorn Alriksson, Leif Jonsson, Venkata Prabhakar Soudham
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Patent number: 8871478Abstract: A Corynebacterium glutamicum transformant having the capability of producing isobutanol and the following genes (1) to (5): (1) a gene which encodes an enzyme having acetohydroxy acid synthase activity; (2) a gene which encodes an enzyme having acetohydroxy acid isomeroreductase activity; (3) a gene which encodes an enzyme having dihydroxy acid dehydratase activity; (4) a gene which encodes an enzyme having 2-keto acid decarboxylase activity; and (5) a gene which encodes an enzyme having alcohol dehydrogenase activity, at least one of the genes being endogenous, and at least one of the genes being exogenous, efficiently produces isobutanol.Type: GrantFiled: March 29, 2010Date of Patent: October 28, 2014Assignee: Research Institute of Innovative Technology for the EarthInventors: Hideaki Yukawa, Masayuki Inui
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Patent number: 8871488Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: GrantFiled: June 15, 2011Date of Patent: October 28, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Publication number: 20140315243Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase, a thermostable protease, and optionally a carbohydrate-source generating enzyme and/or pullulanase, are present and/or added during liquefaction. The invention also relates to compositions suitable for use in a process of the invention.Type: ApplicationFiled: November 30, 2012Publication date: October 23, 2014Inventors: Randall Deinhammer, Joyce Craig, Tomoko Matsui, Shinobu Takagi, Suzanne Clark, John Matthews, Anne Glud Hjulmand, Chee-Leong Soong
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Publication number: 20140315259Abstract: The present application provide methods for producing ethanol from a biomass. The methods combine sugars produced from a feedstock containing starch with sugars produced from a cellulosic biomass. The methods allow increased amounts of ethanol to be produced from a given solids concentration in the fermenters. The methods also encompass filtering the liquefied feedstock mash through a filter comprising biomass fibers. The biomass filter produces a post-filtered mash stream comprising a high concentration of sugars and a low concentration of non-fermentable solids. The methods provide numerous advantages described herein.Type: ApplicationFiled: March 14, 2014Publication date: October 23, 2014Inventors: Richard Root Woods, James Kacmar
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Publication number: 20140315268Abstract: The present invention relates to a method of producing biofuel, more specifically a method of producing biofuel comprising the steps of generating monosugars from marine algae, or from polysaccharides extracted from marine algae by treating the marine algae or the polysaccharides with a hydrolytic enzyme and/or a hydrolytic catalyst; and fermenting the monosugars using a microorganism to produce biofuel. The method of producing biofuel of the present invention solve the problem of raw material suppliance since it uses marine algae as a raw material for biomass, and reduce the production costs by excluding lignin eliminating process that has been required by the conventional method using wood-based raw materials, resulting in economic and environmental advantages.Type: ApplicationFiled: April 2, 2014Publication date: October 23, 2014Inventors: Myung-Kyo SHIN, Yong Jin Kim, Kyeong Keun Oh, Jun Seok Kim, Hyun Jin Ryu, Key Hyup Kim
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Publication number: 20140317786Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 16, 2012Publication date: October 23, 2014Inventor: Marc Morant
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Publication number: 20140308705Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 20, 2012Publication date: October 16, 2014Inventors: Marc Morant, Tarana Shaghasi
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Publication number: 20140308721Abstract: The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: ApplicationFiled: January 17, 2014Publication date: October 16, 2014Applicant: Gevo, Inc.Inventors: Jun Urano, Catherine Asleson Dundon, Peter Meinhold, Reid M. Renny Feldman, Aristos A. Aristidou, Andrew Hawkins, Thomas Buelter, Matthew Peters, Doug Lies, Stephanie Porter-Scheinman, Christopher Smith, Ruth Berry, Ishmeet Kalra
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Publication number: 20140308718Abstract: The invention refers to a method of biotransforming a carbohydrate of a raw material into a chemical, by cultivating Lactobacillus diolivorans in the presence of the raw material to produce a chemical substance, and isolating the chemical substance in the purified form, and the use of L. diolivorans in one of a series of biotransformation methods, wherein carbohydrates from at least two different carbohydrate sources of low purity are transformed into chemicals.Type: ApplicationFiled: November 5, 2012Publication date: October 16, 2014Inventors: Michael Sauer, Hans Marx, Stefan Pfluegl, Diethard Mattanovich
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Publication number: 20140308701Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.Type: ApplicationFiled: August 23, 2012Publication date: October 16, 2014Inventors: Jeffrey Shasky, Amanda Fischer, Suchindra Maiyuran
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Patent number: 8859247Abstract: An enzymatic process is described for the production of chemicals from carbon sources. In particular, according to one aspect, a process for the production of a target organic compound from a carbon source by a cell-free enzyme system is disclosed.Type: GrantFiled: December 28, 2009Date of Patent: October 14, 2014Assignee: Süd-Chemie IP GmbH & Co. KGInventors: Andre Koltermann, Ulrich Kettling, Volker Sieber
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Publication number: 20140302571Abstract: Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol.Type: ApplicationFiled: June 20, 2014Publication date: October 9, 2014Inventors: Marshall MEDOFF, Thomas Craig MASTERMAN
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Publication number: 20140302576Abstract: The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein one or more carbonic anhydrases are present in the fermentation medium.Type: ApplicationFiled: April 7, 2014Publication date: October 9, 2014Applicant: NOVOZYMES NORTH AMERICA, INC.Inventors: Chee Leong Soong, Jiyin Liu, Guillermo Coward Kelly
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Publication number: 20140304859Abstract: Provided are isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 14, 2012Publication date: October 9, 2014Applicant: Novozymes Inc.Inventors: Ye Liu, Yu Zhang, Junxin Duan, Lan Tang
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Patent number: 8852896Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: GrantFiled: June 6, 2013Date of Patent: October 7, 2014Assignee: Xyleco, Inc.Inventor: Marshall Medoff
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Patent number: 8852899Abstract: Embodiments of the invention relate to the enzymatic conversion of bioderived feedstocks to commercially valuable chemicals. The enzymatic conversions of the embodiments of the invention offer the potential for lower cost routes to these value-added chemicals. Some of the chemicals that are useful include nylon intermediates such as caprolactam, adipic acid, 1,6-hexamethylene diamine; butanediols such as 1,4-butanediol, 1,3-butanediol, and 2,3-butanediol; butanols such as 1-butanol, and 2-butanol; succinic acid, butadiene, isoprene, and 3-hydroxypropanoic acid.Type: GrantFiled: June 15, 2012Date of Patent: October 7, 2014Assignee: INVISTA North America S.a.r.l.Inventors: Paul S. Pearlman, Changlin Chen, Adriana L. Botes
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Patent number: 8852907Abstract: The efficient production of ethanol from low-cost biomass (e.g., corn, sugar beets, sugar cane, switchgrass and/or paper) has become increasingly important in making ethanol competitive with gasoline and decreasing the United States' dependence on foreign oil. For example, to reduce the cost of transporting biomass to ethanol production facilities, mobile systems for producing ethanol from biomass are provided. Also provided are small-scale ethanol production facilities. For example, instead of transporting biomass to the production facility, the facility is transported to the biomass or is located nearby the source of the biomass. The ethanol production facilities or components thereof may be transported via land, water, or air. Production of other products, such as hydrocarbons, natural gas, hydrogen gas, plastics, polymers, and proteins, can also be made by the methods and facilities. Any product described herein can be made in finished form or un-finished form and moved, e.g., to a fixed facility, e.g.Type: GrantFiled: February 5, 2013Date of Patent: October 7, 2014Assignee: Xyleco, Inc.Inventor: Marshall Medoff
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Patent number: 8852901Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, methods are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation.Type: GrantFiled: July 15, 2011Date of Patent: October 7, 2014Assignee: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman, Seul-a Bae, Kelly Wallick
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Publication number: 20140295509Abstract: A method of pre-treating a cellulosic material before hydrolysis is provided. The method comprises the steps of: impregnating the cellulosic material with a reactive water-soluble gas, such as sulphur dioxide (SO2) or carbon dioxide (CO2), in an impregnation chamber to obtain impregnated material; and heating the impregnated material to obtain pre-treated material, wherein the cellulosic material is compressed right before or when it is transferred to the impregnation chamber. A corresponding system is also provided.Type: ApplicationFiled: April 29, 2014Publication date: October 2, 2014Applicant: Sekab E-Technology ABInventors: Torbjorn Van Der Meulen, Stanley Forss, Lars Elfving, Staffan Magnusson, Magnus Hagglund, Anders Sjoblom
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Publication number: 20140295512Abstract: The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.Type: ApplicationFiled: June 15, 2012Publication date: October 2, 2014Applicants: CALIFORNIA INSTITUTE OF TECHNOLOGY, GEVO, INC.Inventors: Thomas Buelter, Doug Lies, Stephanie Porter-Scheinman, Christopher Smith, Peter Meinhold, Ruth Berry, Christopher Snow, Sabine Bastian
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Publication number: 20140295513Abstract: The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 80% identical to SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 28, SEQ ID NO: 40, or SEQ ID NO: 58. The present invention also relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 99% identical to SEQ ID NO: 64. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.Type: ApplicationFiled: July 11, 2012Publication date: October 2, 2014Applicants: CALIFORNIA INSTITUTE OF TECHNOLOGY, GEVO, INC.Inventors: Peter Meinhold, Doug Lies, Stephanie Porter-Scheinman, Christopher Smith, Christopher Snow, Sabine Bastian, Sebastian Schoof
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Publication number: 20140287465Abstract: The present invention relates to polypeptides having xylanase activity, catalytic domains, and carbohydrate binding domains, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding domains. The present invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding domains.Type: ApplicationFiled: December 18, 2012Publication date: September 25, 2014Inventors: Nikolaj Spodsberg, Tarana Shaghasi
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Publication number: 20140289905Abstract: Provided are isolated polypeptides having xylanase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: November 9, 2012Publication date: September 25, 2014Applicant: Novozymes Inc.Inventors: Yu Zhang, Junxin Duan, Ye Liu, Lan Tang
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Publication number: 20140273129Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.Type: ApplicationFiled: March 13, 2014Publication date: September 18, 2014Applicant: BUTAMAX ADVANCED BIOFUELS LLCInventors: Ritu Bhalla, Gopal K. Chotani, Michael Dauner, Mark J. Nelson, Daniel P. O'keefe, Caroline M. Peres, Jahnavi Chandra Prasad, Jean-Francois Tomb
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Publication number: 20140273128Abstract: Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH4) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions.Type: ApplicationFiled: March 12, 2014Publication date: September 18, 2014Applicant: Intrexon CorporationInventors: William J. COLEMAN, Genevieve M. VIDANES, Guillaume COTTAREL, Sheela MULEY, Roy KAMIMURA, Akbar F. JAVAN, Jianping SUN, Eli S. GROBAN
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Publication number: 20140272926Abstract: High conversion efficiency processes are disclosed for the anaerobic bioconversion of syngas to alcohol by microorganisms having metabolic processes that utilize sulfur in limited amounts. The processes remove hydrogen sulfide from the gas leaving the bioreactor assembly by forming sulfur compounds that are beneficial to the microorganisms. The sulfur compounds can be returned to the bioreactor assembly to meet a portion of microorganism sulfur demand.Type: ApplicationFiled: March 15, 2013Publication date: September 18, 2014Applicant: COSKATA, INC.Inventor: Richard E. Tobey
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Publication number: 20140273126Abstract: It is an object of the present invention to increase the biomass quantity of plants by causing an exogenous gene to be carried in plant cells. An exogenous gene that promotes double-stranded DNA breakage is introduced into the cells of a plant, and the plant is grown.Type: ApplicationFiled: March 10, 2014Publication date: September 18, 2014Applicants: THE UNIVERSITY OF TOKYO, KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHOInventors: Nobuhiko MURAMOTO, Hiroki SUGIMOTO, Norihiro MITSUKAWA, Kunihiro OHTA, Kazuto KUGOU
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Publication number: 20140273115Abstract: A method is provided for controlling a metabolic profile of an anaerobic microbial fermentation culture. In particular, a metabolic profile of a fermentation process is controlled by controlling the amount of dissolved CO2 provided to a culture. Further provided is a method of producing one or more products by microbial fermentation of a gaseous substrate through feeding tail gas CO2 from a reactor to a second reactor, or by recycling tail gas CO2 to the same reactor.Type: ApplicationFiled: March 12, 2014Publication date: September 18, 2014Applicant: LanzaTech New Zealand LimitedInventors: Sean Dennis Simpson, Michael Koepke, Kathleen Frances Smart, Loan Phuong Tran, Paul Sechrist
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Publication number: 20140273130Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products. Host cells provided herein comprise a pyruvate-utilizing pathway and a competitive growth advantage over other microorganisms in solution.Type: ApplicationFiled: March 14, 2014Publication date: September 18, 2014Applicant: Butamax Advanced Biofuels LLCInventors: Larry Cameron ANTHONY, Michael Dauner
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Publication number: 20140273125Abstract: Processes are disclosed for the anaerobic bioconversion of syngas to oxygenated organic compound that use an in situ method for protecting the microorganisms from hydrogen cyanide contained in the syngas that passes to the fermentation broth. The fermentation broth is maintained at a pH of between about 4 and 6, and dissolved metal cation of one or more of iron, cobalt, nickel and zinc is provided to the fermentation broth in an amount sufficient to form, under the conditions of the fermentation broth, a substantially insoluble metal complex with the metal cation and cyanide anion. The rate of formation of the insoluble complex is sufficiently high that that the amount of cyanide that is taken up by microorganisms does not result in an undue adverse effect on the population of microorganisms.Type: ApplicationFiled: March 14, 2013Publication date: September 18, 2014Applicant: COSKATA, INC.Inventors: Robert Hickey, Jianxin Du, Andrew Reeves, Richard E. Tobey
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Publication number: 20140273105Abstract: Lignocellulosic biomass pretreated with ammonia where the concentration of the biomass in the reaction mixture is reduced over time was found to produce more sugars following saccharification, as compared to equivalent biomass pretreated at constant concentration. The concentration of biomass is a solids concentration, which is the percent of dry biomass relative to the total pretreatment reaction mixture on a weight to weight basis.Type: ApplicationFiled: March 5, 2014Publication date: September 18, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: PATRICIA CHEUNG, BRADLEY CURT FOX, MING WOEI LAU, JOSEPH MICHAEL SELBY, GREGORY PAUL SHANKWITZ, STUART M THOMAS, RYAN ERIC WARNER
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Publication number: 20140273127Abstract: The present invention relates to processes and systems for the production of fermentative products such as ethanol and butanol. The present invention also provides methods for separating feed stream components for improved biomass processing productivity.Type: ApplicationFiled: March 11, 2014Publication date: September 18, 2014Applicant: BUTAMAX ADVANCED BIOFUELS LLCInventors: Benjamin Fuchs, BRIAN MICHAEL ROESCH, MATHIAS E. STOLARSKI, JAMES GREGORY WOOD
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Publication number: 20140256006Abstract: The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.Type: ApplicationFiled: May 21, 2014Publication date: September 11, 2014Applicant: Commonwealth Scientific and Industrial Research OrganisationInventors: Thomas Vanhercke, James Robertson Petrie, Anna El Tahchy, Surinder Pal Singh, Qing Liu
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Publication number: 20140256001Abstract: Systems and methods for cooling and processing materials are disclosed.Type: ApplicationFiled: May 20, 2014Publication date: September 11, 2014Applicant: XYLECO, INCInventor: Marshall Medoff
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Publication number: 20140256000Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: October 31, 2012Publication date: September 11, 2014Inventors: Lan Tang, Ye Liu, Yu Zhang, Junxin Duan, Tarana Shaghasi
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Patent number: 8828695Abstract: A method of making butanol from at least one fermentable carbon source that overcomes the issues of toxicity resulting in an increase in the effective titer, the effective rate, and the effective yield of butanol production by fermentation utilizing a recombinant microbial host wherein the butanol is extracted into specific organic extractants during fermentation.Type: GrantFiled: April 13, 2010Date of Patent: September 9, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Michael Charles Grady, Mehmedalija Jahic, Ranjan Patnaik
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Patent number: 8828694Abstract: Yeast cells with modified expression of certain enzyme activities in the mitochondria are described for isobutanol production. Modifications described provide an isobutanol biosynthesis pathway in the yeast mitochondria.Type: GrantFiled: November 12, 2009Date of Patent: September 9, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Larry Cameron Anthony, Lixuan Lisa Huang, Rick W. Ye
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Patent number: 8828704Abstract: Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. Specifically disclosed herein are the use of coenzyme B12-independent butanediol dehydratases that catalyzes the substrate to product conversion of 2,3-butanediol to 2-butanone in the process of producing 2-butanol and 2-butanone.Type: GrantFiled: April 29, 2008Date of Patent: September 9, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Gail K. Donaldson, Andrew C. Eliot, Lori Ann Maggio-Hall, Vasantha Nagarajan, Charles E. Nakamura, Jean-Francois Tomb
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Publication number: 20140248663Abstract: Provided are isolated polypeptides having cellobiohydrolase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: November 15, 2012Publication date: September 4, 2014Inventors: Lan Tang, Junxin Duan, Yu Zhang, Ye Liu
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Publication number: 20140242657Abstract: This invention describes a process for the production of alcohols and/or solvents from cellulosic or lignocellulosic biomass that comprises at least the following stages: a) Alkaline chemical pretreatment based on sodium sulfate of a cellulosic or lignocellulosic substrate; b) Washing of the pretreated substrate; c) Enzymatic hydrolysis of the substrate that is pretreated and washed using cellulolytic and/or hemicellulolytic enzymes that produce a hydrolyzate and a water-insoluble residue; d) Microorganism fermentation of the hydrolyzate that is obtained from stage c) and production of a fermentation must that contains at least one alcohol and/or solvent; e) Separation/purification of alcohol and/or solvent, and f) Separation of a cake that contains the insoluble residue, in which at least a portion of the cake that is obtained in stage f) is recycled upstream from the pretreatment stage a) and/or upstream from the washing stage b).Type: ApplicationFiled: May 5, 2014Publication date: August 28, 2014Applicant: IFP Energies nouvellesInventors: Marcel ROPARS, Caroline AYMARD