Carrier Is Carbohydrate Patents (Class 435/178)
  • Patent number: 7935334
    Abstract: An exemplary embodiment providing one or more improvements includes feeding animals with probiotic microbes encapsulated in a mixture of xanthan gum and chitosan, or in gelatin, specifically Pediococcus acidilactici and Saccharomyces boulardii. Such encapsulation protects the viability of the probiotic microbes against unfavorable temperatures. An exemplary embodiment providing one or more improvements includes methods of using viable probiotics in therapy of birds and mammals infected with infectious diseases. Probiotics acted as adjuvants in stimulating antibody reaction and stimulated a cellular immunity response. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.
    Type: Grant
    Filed: July 26, 2006
    Date of Patent: May 3, 2011
    Assignee: Imagilin Technologies, LLC
    Inventor: Jhy-Jhu Lin
  • Publication number: 20110091955
    Abstract: Systems and methods for lowering levels of carbon dioxide and other atmospheric pollutants are provided. Economically viable systems and methods capable of removing vast quantities of carbon dioxide and other atmospheric pollutants from gaseous waste streams and sequestering them in storage stable forms are also discussed.
    Type: Application
    Filed: October 19, 2010
    Publication date: April 21, 2011
    Inventors: BRENT R. CONSTANTZ, James Bresson, Divyam Chandra
  • Publication number: 20110081677
    Abstract: The present invention relates to a biofabricated Active Microfluidic Membrane (AMM) in a microfluidic network of a microfluidic device and a method for the in situ biofabrication of such a microfluidic network. More specifically, the invention relates to devices exhibiting (and methods of) positioning (i.e., erecting, modifying or removing a membrane matrix in situ in a microchannel of a microfluidic network of a microfluidic device. In one embodiment, the membrane comprises a single type of matrix constituent, such as chitosan, alginate, etc. Alternatively, the membrane may be composed of two or more matrix constituents, which may be integrated into one another or layered adjacent to one another.
    Type: Application
    Filed: September 24, 2010
    Publication date: April 7, 2011
    Applicant: University of Maryland, College Park
    Inventors: Xiaolong Luo, Gary W. Rubloff, Dean L. Berlin, William E. Bentley, Susan Buckhout-White, Yi Cheng, Jordan Betz
  • Publication number: 20110076737
    Abstract: This patent involves improvement of the thermal stability of natural polymers, “hydrogels”, to be used as supports for covalent immobilization of enzymes. The presence of the new functionality has been proved by FTIR. In brief, the new polymeric carrier has the main following advantages: • Natural polymer & friendly to the environment • Highly hydrophilic • Containing active groups for covalent immobilization of enzymes& antibodies Thermally stable (35-95° C.) Porous The modified carrier could be used for immobilization of enzymes on the industrial scale. The immobilized enzymes could be reused for tens of times, which will reduce the cost of the enzymes and their products.
    Type: Application
    Filed: June 11, 2009
    Publication date: March 31, 2011
    Inventor: Magdy Mahmoud Mustafa Elnashar
  • Publication number: 20110059497
    Abstract: A process for converting biomass hydrolysate into biofuel, the process comprising the steps of: obtaining a biomass hydrolysate solution comprising monosaccharides; immobilizing Pachysolen tannophilus; contacting the solution with the immobilized Pachysolen tannophilus; and recovering a fermented biofuel.
    Type: Application
    Filed: August 13, 2010
    Publication date: March 10, 2011
    Inventors: Lisa Beckler Andersen, John H. Evans, IV, Christine A. Singer
  • Patent number: 7888079
    Abstract: The present invention provides an aminoacylase having superior abilities in specifically acylating and hydrolyzing e-amino group of Lys, and a method of producing N?-acyl-L-lysine. The present invention provides N?-acyl-L-lysine-specific aminoacylase containing the amino acid sequence of SERPXTTLLRNGDVH (X unknown) at the N-terminal, and a method of producing N?-acyl-L-lysine comprising acting the aminoacylase on L-Lys and a carboxylic acid.
    Type: Grant
    Filed: August 21, 2007
    Date of Patent: February 15, 2011
    Assignees: Ajinomoto Co., Inc., National University Corporation Okayama University
    Inventors: Kazuhiro Nakanishi, Koreyoshi Imamura, Hiroyuki Imanaka, Mayuko Koreishi, Noriki Nio
  • Publication number: 20110000854
    Abstract: A method uses anionic and cationic polymers added in, any order or simultaneously, to aqueous media for the removal of substances. The sequential addition of the two biopolymers, anionic xanthan, followed by cationic chitosan, causes the rapid formation of very large and cohesive fibrillar aggregates that may exhibit high solids to liquid ratios and that quickly settle out from the aqueous media. The aqueous media can be easily separated from the large fibrillar aggregates by settling under gravity or by filtration through a porous containment device, such as a synthetic or non-synthetic woven or non-woven fabric including a geotextile fabric or a solid containment device containing a solid mesh screen.
    Type: Application
    Filed: July 6, 2010
    Publication date: January 6, 2011
    Applicant: HaloSource, Inc.
    Inventors: Everett J. Nichols, James R. Scott, Jeffrey F. Williams, Yongjun Chen
  • Publication number: 20100297720
    Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation.
    Type: Application
    Filed: May 18, 2010
    Publication date: November 25, 2010
    Applicant: XYLECO, INC.
    Inventors: Marshall Medoff, Thomas Craig Masterman, Harrison D. Medoff
  • Publication number: 20100279375
    Abstract: A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.
    Type: Application
    Filed: July 20, 2010
    Publication date: November 4, 2010
    Applicant: KAO CORPORATION
    Inventors: Manabu SATO, Kazuhiro ONOZUKA, Takaaki WATANABE, Hiroyuki KONISHI
  • Publication number: 20100272812
    Abstract: The object of the present invention is to provide particles of a polymeric material containing cells therein, wherein said particles have a strength that is substantially greater than the microcapsules known in the state of the art.
    Type: Application
    Filed: June 25, 2008
    Publication date: October 28, 2010
    Applicant: UNIVERSIDAD DEL PAIS VASCO
    Inventors: Gorka Orive Arroyo, José Luis Pedraz Muñoz, Rosa María Hernández
  • Patent number: 7815926
    Abstract: An implant for articular cartilage repair includes (1) a three-dimensional body formed of cancellous bone having a demineralized section that contains bone morphogenetic proteins (BMP's) that are released by the demineralization but retained in the body, and (2) a cartilage layer formed on a surface of the demineralized section. The cartilage layer is formed by a method including the steps of (a) isolating chondrocytes from articular cartilage of a donor; (b) cultivating the isolated chondrocytes in a medium; (c) suspending the cultivated chondrocytes in agarose; (d) adding the cultivated chondrocytes to the demineralized section of the body, whereby the cultivated chondrocytes are stimulated by the BMP's retained in the body; and (e) incubating the cultivated chondrocytes to form a plurality of layers of chondrocytes on the demineralized section, wherein the plurality of layers of chondrocytes forms the cartilage layer.
    Type: Grant
    Filed: July 7, 2006
    Date of Patent: October 19, 2010
    Assignee: Musculoskeletal Transplant Foundation
    Inventors: Carina Syring, Rudiger Walter Arthur von Versen
  • Patent number: 7807150
    Abstract: Slowly polymerizing polysaccharide hydrogels have been demonstrated to be useful as a means of delivering large numbers of isolated cells via injection. The gels promote engraftment and provide three dimensional templates for new cell growth. The resulting tissue is similar in composition and histology to naturally occurring tissue. This method can be used for a variety of reconstructive procedures, including custom molding of cell implants to reconstruct three dimensional tissue defects, as well as implantation of tissues generally.
    Type: Grant
    Filed: March 8, 2004
    Date of Patent: October 5, 2010
    Assignees: Massachusetts Institute of Technology, Children's Medical Center Corporation
    Inventors: Linda G. Griffith, Anthony Atala, Charles A. Vacanti, Keith T. Paige
  • Patent number: 7807428
    Abstract: A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.
    Type: Grant
    Filed: November 10, 2005
    Date of Patent: October 5, 2010
    Assignee: Kao Corporation
    Inventors: Manabu Sato, Kazuhiro Onozuka, Takaaki Watanabe, Hiroyuki Konishi
  • Publication number: 20100248229
    Abstract: The present invention relates to the field of stem cells particularly development of a novel human embryonic model using human embryoid bodies obtained from the human embryonic stem cell. The novel human embryonic model disclosed thus can provide a screening assay for determining the toxic activity of the compound and/or drug.
    Type: Application
    Filed: April 11, 2008
    Publication date: September 30, 2010
    Applicant: Stempeutics Research Private Limited
    Inventors: Kaushik Dilip Deb, Satish Mahadeo Totey
  • Publication number: 20100249250
    Abstract: The invention provides the use of novel, binary guanosine gels for simple, rapid and nondestructive solubilization of individual single walled carbon nanotubes (SWNTs) at high concentrations. The gels exhibit selectivity between metallic and semiconducting SWNTs and, further, among SWNTs with different chiralities.
    Type: Application
    Filed: June 11, 2010
    Publication date: September 30, 2010
    Applicant: Rensselaer Polytechnic Institute
    Inventors: Linda McGown, Omkaram Nalamasu, Yuehua Yu
  • Publication number: 20100239540
    Abstract: Biostructures that comprises modified alginates entrapping one or more stem cells are discloses. The modified alginates comprise at least one alginate chain section to which is bonded by covalent bonding at least one cell attachment peptide. Pluralities of stem cells are also disclosed. Methods of preventing death of stem cells and cells differentiated there from are disclosed. Methods of preparing a plurality of stem cells are disclosed. Methods of treating an individual who has a degenerative disease, such as a neurological disorder, or injury involving nerve damage by administering stem cells to said individual are disclosed.
    Type: Application
    Filed: June 13, 2008
    Publication date: September 23, 2010
    Applicant: FMC CORPORATION
    Inventors: Jan Engelsen Brinchmann, Katrine Bjornebek Fronsdal, Jan Egil Melvik
  • Publication number: 20100221301
    Abstract: The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprise the steps consisting of a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide and one cross-linking agent b) freezing the aqueous solution of step a) c) sublimating the frozen solution of step b). characterized in that step b) is performed before the cross-linking of the polysaccharide occurs in the solution of step a).
    Type: Application
    Filed: October 10, 2008
    Publication date: September 2, 2010
    Applicants: UNIVERSITE PARIS 7 - DENIS DIDEROT, RECHERCHE MEDICALE)
    Inventors: Catherine Le Visage, Didier Letourneur, Frederic Chaubet, Aude Autissier
  • Publication number: 20100183569
    Abstract: The present invention refers to a porous composite material, wherein at least one interdispersed biopolymer is present, with a calcium-phosphate mineral component comprising from 50 w/% to 95 w/% of ?-tricalcium phosphate (?-TCP, ?-Ca3(PO4)2) and from 5 w/% to 50 w/% of octacalcium phosphate (OCP, Ca8H2(PO4)6—5H2O), to the total weight of the mineral component. Such combination of ?-TCP and OCP allows an in vivo faster resorption rate and thereby a faster formation of new bone tissue having a low cristallinity mineral component with nanocrystal structure, features very similar to those of biological apatites. This porous composite material may find application as bone and/or osteal-chart ilagineous substitute (scaffold) and in producing tissue engineering devices.
    Type: Application
    Filed: June 27, 2008
    Publication date: July 22, 2010
    Applicants: ALMA MATER STUDIORUM - UNIVERSITA' DI BOLOGNA, ISTITUTO ORTOPEDICO RIZZOLI
    Inventors: Adriana Bigi, Silvia Panzavolta, Roberto Giardino, Milena Fini
  • Publication number: 20100176053
    Abstract: A modified bioreactor support material having high surface area for removing a contaminant (16) from fluids can include a substrate (10) having a functionalized surface, The functionalized surface can have inorganic or organic non-living functional groups, such that the functional groups bind to or chemically alter the contaminant. A method for making a modified bioreactor support material can include activating a suitable substrate (10) and attaching a biologically-derived functional group carrier such as living microbes (18) or non-living materials (14) derived from living materials to the activated substrate (10).
    Type: Application
    Filed: March 28, 2008
    Publication date: July 15, 2010
    Inventors: D. Jack Adams, Jan D. Miller
  • Publication number: 20100167300
    Abstract: The present invention provides three-dimensional microenvironment niches prepared from biomaterial compositions that supports growth and self renewal of stem cells. The invention also provides methods for inducing pluripotency in a somatic cell using chemical compounds, as well as methods for screening for compounds that can induce pluripotency in a somatic cell.
    Type: Application
    Filed: December 14, 2009
    Publication date: July 1, 2010
    Inventor: Babak Esmaeli-Azad
  • Publication number: 20100136647
    Abstract: The present invention relates to the field of adherent cell culture. More closely, the invention relates to a method for production of a cell attachment and culture surface, such as a microcarrier, comprising a guanidino-containing ligand, wherein the ligand is coupled via reaction involving a primary amine to the surface which is activated by activation groups such that the final molar ratio of grafted ligand and ungrafted activation groups is above 1.5. Preferably, the ligand density is above 0.5 mmol/g cell culture surface and the remaining activation groups after coupling is less than 0.6 mmol/g cell culture surface. The cell culture surface may be used for various purposes, primarily cell cultivation and virus production.
    Type: Application
    Filed: November 23, 2009
    Publication date: June 3, 2010
    Applicant: GE HEALTHCARE BIO-SCIENCES AB
    Inventors: Mattias Algotsson, Hans Berg, Asa Bjurling, Christian Kaisermayer, Bjorn Noren, Nicholas Thevenin, James Van Alstine
  • Patent number: 7723086
    Abstract: An apparatus for encapsulating cells is disclosed. According to one embodiment, the apparatus includes an indirect-pumping dispenser for dispensing a cell suspension into an encapsulation solution through an outlet of the dispenser. The apparatus also includes a dipping mechanism that is attachable to the outlet of the dispenser. The dipping mechanism is adapted to dip the dispenser outlet in the encapsulation solution to allow the cell suspension dispensed thereat to come into contact with the encapsulation solution.
    Type: Grant
    Filed: June 16, 2004
    Date of Patent: May 25, 2010
    Assignee: Agency for Science, Technology + Research
    Inventors: Hanry Yu, Hai Ting Ho, Jing Zhang
  • Patent number: 7700355
    Abstract: A porous matrix, preparation thereof, and methods of using the same. The porous matrix of calcium alginate is prepared using a plurality of particles containing a multivalent cation, admixing the particles with ionic cross-linked polysaccharides and water to form a mixture, introducing a cross linker to solidify the mixture, dissolving the particles of the mixture in an acid to form a porous structure, and neutralizing and cross-linking the porous structure.
    Type: Grant
    Filed: January 7, 2005
    Date of Patent: April 20, 2010
    Assignee: Industrial Technology Research Institute
    Inventors: Chun-Jen Liao, Jui-Hsiang Chen, Chen-Chi Tsai, Yung-Chih Wu, Shu-Fang Chiang, Yi-Jung Hsiang
  • Publication number: 20100093053
    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 ?m and 400 ?m, such as about 200 ?m. It may have a cross sectional dimension of between 20 ?m and 30 ?m. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 ?m and about 120 ?m, for example about 65 ?m. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage.
    Type: Application
    Filed: July 3, 2009
    Publication date: April 15, 2010
    Applicant: Agency for Science, Technology and Research
    Inventors: Steve Oh, Marti Lecina, Andre Choo, Shaul Reuveny, Robert Zweigert, Allen Chen
  • Patent number: 7691988
    Abstract: A method is provided for nucleic acid amplification with enhanced sensitivity. The method for enhanced sensitivity involves carrying out the amplification reaction in the presence of gellan. For instance, the method allows for the production of detectible amounts of PCR amplified DNA from at least 10 fold fewer target molecules than a comparable PCR reaction in absence of gellan.
    Type: Grant
    Filed: November 20, 2003
    Date of Patent: April 6, 2010
    Assignee: Shin-Etsu Chemical Company, Ltd.
    Inventor: Richard W Armentrout
  • Patent number: 7648831
    Abstract: The purpose of the present invention is to provide a process for protection of enzymatic biocatalysts, constituted by a primary support, wrapped by a secondary matrix. More particularly, the invention is embodied as a bioreactor with a configuration that preserves the physical integrity and catalytic activity of the biocatalyst after long runs, and at the same time allows an easy separation of the protected biocatalyst from the solid, precipitated during the reaction course. Still more particularly, the invention refers to the use of the bioreactor herein described for the enzymatic synthesis of ?-lactam antibiotics, including the insoluble enzymatic catalysts, which have a primary matrix for immobilization involved by a secondary matrix, which is able to preserve 100% of the catalyst physical integrity and approximately 100% of its catalytic activity.
    Type: Grant
    Filed: November 24, 2003
    Date of Patent: January 19, 2010
    Assignees: Fundacao Universidade Federal de Sao Carlos, Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
    Inventors: Roberto de Campos Giordano, Raquel de Lima Camargo Giordano, Andrea Lopes de Oliveira Ferreira
  • Publication number: 20090325263
    Abstract: The invention related to a method of making a dried reagent preparation, comprising the steps of: providing an aqueous solution of at least one buffered biological reagent; mixing a glass forming filler material with the buffered reagent solution to form a mixture wherein the concentration of the filler material is sufficient to facilitate formation of a glassy, porous composition; dispensing the mixture in the form of substantially uniform droplets into wells of a multi-well container, wherein a single droplet is dispensed into each well; drying the droplets in the container to form the reagent preparation. The reagent preparation is water soluble and has a Tg sufficient for room temperature stability.
    Type: Application
    Filed: September 13, 2007
    Publication date: December 31, 2009
    Applicant: GE HEALTHCARE BIO-SCIENCES CORP.
    Inventors: Reddy Ponaka, Joseph W. Farchaus, Michael D. Pierce
  • Publication number: 20090286278
    Abstract: A multi-layered microcapsule has an inner extracellular matrix and an outer shell. The inner extracellular matrix includes a first inner layer of biopolymer and a second intermediate layer of polymer that provides partial immune-protection and holds the first layer in place. The outer shell can form an exoskeleton to provide mechanical stability. Each of the individual layers can be varied to optimize mechanical stability, cell function, and immuno-protection.
    Type: Application
    Filed: July 23, 2009
    Publication date: November 19, 2009
    Applicants: Agency for Science, Technology and Research, National University of Singapore
    Inventors: Hanry Yu, Kam W. Leong, Ser-Mien Chia, Andrew C.A. Wan
  • Patent number: 7618646
    Abstract: The invention relates to porous matrices based on a biologically compatible polymer or polymer mixture, to a cell implantation that is established on said matrices and to additional cell implantation based on cell mixtures of hepatocytes and islets of Langerhans. The invention also relates to a method for producing porous matrices, to matrices obtained according to said method and to a special method for obtaining cells for the inoculation of an implantable matrix.
    Type: Grant
    Filed: June 7, 2004
    Date of Patent: November 17, 2009
    Assignee: HUMANAUTOCELL GmbH
    Inventors: Martin Goerne, Peter Matthias Kaufmann
  • Publication number: 20090275637
    Abstract: The present invention provides compositions and methods for binding and/or modulating enzymatic activity of human protein tyrosine phosphatases such as PTP1B. Additionally, the invention provides methods of identifying and using such nucleic acid ligands.
    Type: Application
    Filed: February 5, 2009
    Publication date: November 5, 2009
    Inventors: Brent Townshend, Michel Tremblay
  • Patent number: 7611701
    Abstract: A process for the preparation of an enzyme-containing granulate is disclosed where an aqueous enzyme-containing liquid is mixed with an edible carbohydrate-based solid carrier, such as starch, mechanically processed into granules, and subsequently dried. This enzyme granulate is suitable for the manufacture of animal feed compositions by mixing feed ingredients with the granulate, treating with steam and pelleting. The compositions optimally show improved enzyme stability during the pelleting process.
    Type: Grant
    Filed: October 25, 2002
    Date of Patent: November 3, 2009
    Assignee: BASF Aktiengesellschaft
    Inventors: Rudolph Carolus Maria Barendse, Gabriel Marinus Henricus Meesters, Hans-Peter Harz
  • Patent number: 7601525
    Abstract: The present invention provides copper capillary alginate gels stabilized with barium, chitosan, its derivates, or a combination thereof. These stabilized gels are useful as scaffolds for containing, growing, or regenerating biological agents and cells for in vivo or in vitro use.
    Type: Grant
    Filed: March 7, 2005
    Date of Patent: October 13, 2009
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Christopher D. Batich, Bradley Jay Willenberg, Takashi Hamazaki, Naohiro Terada
  • Publication number: 20090208474
    Abstract: The present invention provides engineered enzymes generated from protein scaffolds combined with Specificity Determining Regions, the production thereof and the use of said engineered enzymes for research, nutritional care, personal care and industrial purposes.
    Type: Application
    Filed: December 21, 2007
    Publication date: August 20, 2009
    Inventors: Ulrich Haupts, Andre Koltermann, Andreas Scheidig, Christian Votsmeier, Ulrich Ketting
  • Publication number: 20090203103
    Abstract: The present invention is directed to methods for inducing desired activity in enzymes or microorganisms capable of producing the enzymes. The invention is further directed to methods of stabilizing activity in microorganisms. In specific embodiments, the invention provides methods for inducing and stabilizing nitrile hydratase activity, amidase activity, and asparaginase I activity. The invention further provides compositions comprising enzymes or microorganisms having induced and/or stabilized activity.
    Type: Application
    Filed: April 3, 2009
    Publication date: August 13, 2009
    Applicant: GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventors: George E. Pierce, Gene K. Drago, Sangeeta Ganguly
  • Patent number: 7572456
    Abstract: The present invention relates to compositions comprising humanized antibodies or fragments thereof that immunospecifically bind to one or more antigens of a flavivirus, particularly of West Nile Virus (WNV) and methods for preventing, treating or ameliorating symptoms associated with a flavivirus, particularly of West Nile Virus (WNV) infection utilizing said compositions. In particular, the present invention relates to methods for preventing, treating or ameliorating symptoms associated with WNV infection, said methods comprising administering to a human subject an effective amount of one or more humanized antibodies or fragments thereof that immunospecifically bind to a WNV antigen. The present invention also relates to detectable or diagnostic compositions comprising humanized antibodies or fragments thereof that immunospecifically bind to a WNV antigen and methods for detecting or diagnosing WNV infection utilizing said compositions.
    Type: Grant
    Filed: September 13, 2005
    Date of Patent: August 11, 2009
    Assignee: MacroGenics, Inc.
    Inventors: Leslie S. Johnson, Ling Huang
  • Patent number: 7547449
    Abstract: The subject invention concerns a composite comprising an organic fluid-swellable, fibrous matrix, such as collagen, and a mineral phase, such as calcium carbonate or phosphate mineral phase, for use as a biomimetic of bone. In another aspect, the subject invention concerns a process for making a composite involving the inclusion of acidic polymers to a supersaturated mineralizing solution, in order to induce an amorphous liquid-phase precursor to the inorganic mineral, which is then absorbed (pulled by capillary action) into the organic matrix. Advantageously, once solidified, a high mineral content can be achieved, with the inorganic mineral crystals embedded within the collagen fibers (intrafibrillarly) and oriented such that they are aligned along the long axes of the fibers of the organic matrix, thereby closely mimicking the natural structure of bone.
    Type: Grant
    Filed: May 12, 2006
    Date of Patent: June 16, 2009
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Laurie B. Gower, Matthew J. Olszta, Elliot P. Douglas, Sivakumar Munisamy, Donna L. Wheeler
  • Patent number: 7544496
    Abstract: The subject invention concerns a composite comprising an organic fluid-swellable, fibrous matrix, such as collagen, and a mineral phase, such as calcium carbonate or phosphate mineral phase, for use as a biomimetic of bone. In another aspect, the subject invention concerns a process for making a composite involving the inclusion of acidic polymers to a supersaturated mineralizing solution, in order to induce an amorphous liquid-phase precursor to the inorganic mineral, which is then absorbed (pulled by capillary action) into the organic matrix. Advantageously, once solidified, a high mineral content can be achieved, with the inorganic mineral crystals embedded within the collagen fibers (intrafibrillarly) and oriented such that they are aligned along the long axes of the fibers of the organic matrix, thereby closely mimicking the natural structure of bone.
    Type: Grant
    Filed: May 12, 2006
    Date of Patent: June 9, 2009
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Laurie B. Gower, Matthew J. Olszta, Elliot P. Douglas, Sivakumar Munisamy, Donna L. Wheeler
  • Publication number: 20090136827
    Abstract: Bioanodes, biocathodes, biofuel cells, immobilized enzymes and immobilization materials comprising a micellar hydrophobically modified polysaccharide are disclosed. In particular, the micellar hydrophobically modified polysaccharide can be a hydrophobically modified chitosan or a hydrophobically modified alginate.
    Type: Application
    Filed: November 2, 2006
    Publication date: May 28, 2009
    Applicant: ST. LOUIS UNIVERSITY
    Inventors: Shelley D. Minteer, Tamara L. Klotzbach, Rodica Duma
  • Publication number: 20090098628
    Abstract: Devices for the microencapsulation of cells include a first chamber for containing a cell-solution suspension. A plate covers one end of the first chamber. The plate has a plurality of apertures. A second chamber is provided for receiving encapsulated cells. The second chamber is separated from the first chamber by the plate. Cells from the first chamber are encapsulated by passing through the apertures in the plate and into the second chamber when pressure is applied to the cell-solution suspension.
    Type: Application
    Filed: December 18, 2008
    Publication date: April 16, 2009
    Inventor: M.K. Ramasubramanian
  • Publication number: 20090095677
    Abstract: Provided are a kit including a first component that is a compound of formula (1) below bound to a first material and a second component that is a ligand bound to a second material, wherein each of the first and second materials is independently selected from the group consisting of a solid phase, a biomolecule, an antioxidant, a chemical therapeutic agent, an anti-histaminic agent, a cucurbituril dendrimer, a cyclodextrin derivative, a crown ether derivative, a calixarene derivative, a cyclophane derivative, a cyclic peptide derivative, a metallic ion, a chromophore, a fluorescent material, a phosphor, a radioactive material, and a catalyst; and the ligand can non-covalently bind to the compound of formula (1); a method of separating and purifying a material bound to a ligand using the compound of formula (1) bound to a solid phase; a method of separating and purifying the compound of formula (1) or a material bound to the compound using a ligand bound to a solid phase; a sensor chip including a compound of f
    Type: Application
    Filed: December 19, 2008
    Publication date: April 16, 2009
    Applicants: POSTECH FOUNDATION, POSTECH ACADEMY-INDUSTRY FOUNDATION
    Inventors: Kimoon KIM, Kangkyun Baek, Jeeyeon Kim, Ilha Hwang, Young Ho Ko, Narayanan Selvapalam, Erumaipatty R. Nagarajan, Kyeng Min Park
  • Patent number: 7514248
    Abstract: The subject invention concerns a composite comprising an organic fluid-swellable matrix, such as collagen, and mineral phase, such as calcium carbonate or phosphate mineral phase, for use as a biomimetic of bone. In another aspect, the subject invention concerns a process for making a composite involving the inclusion of acidic polymers to a supersaturated mineralizing solution, in order to induce an amorphous liquid-phase precursor to the inorganic mineral, which is then absorbed (pulled by capillary action) into the interstices of the organic matrix, and subsequently mineralizes via solidification and crystallization of the precursor phase. The present invention further concerns a method of treating a patient suffering from a bone defect by applying a biomimetic composite to the bone defect site.
    Type: Grant
    Filed: April 18, 2003
    Date of Patent: April 7, 2009
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Laurie B. Gower, Matthew J. Olszta, Elliot P. Douglas, Sivakumar Munisamy, Donna L. Wheeler
  • Patent number: 7514540
    Abstract: The invention concerns a method for immobilizing biomolecules on a polymeric surface of a carrier material and a carrier material functionalized with a biomolecule according to the general formulas (IV) and (V), which can be produced according to the method, wherein P indicates the polymeric surface of the carrier material and M indicates the biomolecule bound to the linker compound via an amino group (formula IV) or a carbonyl group (formula V) and R2 has the meaning OR4 or NR4R5; R1, R4 and R5, independently of one another, indicate H, and alkyl group or an aryl group; R3 indicates H, an alkyl, an aryl, an acyl, an alkoxycarbonyl or an aryloxycarbonyl group; and the alkyl, aryl, acyl, alkoxycarbonyl and/or aryloxycarbonyl group of the radicals R1, R3, R4 and R5, independently of one another, are substituted or unsubstituted.
    Type: Grant
    Filed: December 3, 2001
    Date of Patent: April 7, 2009
    Assignee: Poly-An Gesellschaft zur Herstellung Von Polymeren Fuer Spezielle Anwendungen und Analytik mbH
    Inventors: Pablo Wessig, Jürgen Bendig, Uwe Schedler
  • Patent number: 7514249
    Abstract: The subject invention concerns a composite comprising an organic fluid-swellable, fibrous matrix, such as collagen, and a mineral phase, such as calcium carbonate or phosphate mineral phase, for use as a biomimetic of bone. In another aspect, the subject invention concerns a process for making a composite involving the inclusion of acidic polymers to a supersaturated mineralizing solution, in order to induce an amorphous liquid-phase precursor to the inorganic mineral, which is then absorbed (pulled by capillary action) into the organic matrix. Advantageously, once solidified, a high mineral content can be achieved, with the inorganic mineral crystals embedded within the collagen fibers (intrafibrillarly) and oriented such that they are aligned along the long axes of the fibers of the organic matrix, thereby closely mimicking the natural structure of bone.
    Type: Grant
    Filed: October 22, 2003
    Date of Patent: April 7, 2009
    Assignee: The University of Florida Research Foundation, Inc.
    Inventors: Laurie B. Gower, Matthew J. Olszta, Elliot P. Douglas, Sivakumar Munisamy, Donna L. Wheeler
  • Publication number: 20090053776
    Abstract: The present invention is directed to methods and systems of producing neoagarobiose, useful in whitening melanoma cells and in cosmetics, using polypeptides having neoagarobiosebiohydralase activity, including Aga86E from Saccharophagus degradans. The reaction can be enhanced by including other agarases, including Aga16B, also from S. degradans.
    Type: Application
    Filed: April 30, 2008
    Publication date: February 26, 2009
    Inventors: Steven W. HUTCHESON, Ronald M. WEINER, Nathan A. EKBORG
  • Patent number: 7482152
    Abstract: Devices for the microencapsulation of cells include a first chamber for containing a cell-solution suspension. A plate covers one end of the first chamber. The plate has a plurality of apertures. A second chamber is provided for receiving encapsulated cells. The second chamber is separated from the first chamber by the plate. Cells from the first chamber are encapsulated by passing through the apertures in the plate and into the second chamber when pressure is applied to the cell-solution suspension.
    Type: Grant
    Filed: January 7, 2005
    Date of Patent: January 27, 2009
    Inventor: Melur Ramasubramanian
  • Publication number: 20090022702
    Abstract: Methods to introduce highly phosphorylated mannopyranosyl oligosaccharide derivatives containing mannose 6-phosphate (M6P), or other oligosaccharides bearing other terminal hexoses, to carbonyl groups on oxidized glycans of glycoproteins while retaining their biological activity are described. The methods are useful for modifying glycoproteins, including those produced by recombinant protein expression systems, to increase uptake by cell surface receptor-mediated mechanisms, thus improving their therapeutic efficacy in a variety of applications.
    Type: Application
    Filed: September 24, 2008
    Publication date: January 22, 2009
    Inventor: Yunxiang ZHU
  • Publication number: 20090004151
    Abstract: It is intended to provide a method of three-dimensionally culturing normal joint chondrocytes; the production and supply of chondrocytes; and a transplantation material to be used in an injured site in a joint tissue.
    Type: Application
    Filed: June 1, 2005
    Publication date: January 1, 2009
    Inventors: Shusa Ohshika, Keiichi Takagaki, Satoshi Toh, Yasuyuki Ishibashi, Takagaki Yoko
  • Publication number: 20080262605
    Abstract: Hybrid synthetic grafts and embodiments of systems and methods for producing hybrid vascular grafts that can yield implantable grafts that combine synthetic grafts with living cells. Embodiments of systems can include a pressure/flow loop subsystem having an external flow loop system coupled to a specimen holder, where the pressure/flow loop subsystem is capable of adjusting at least two dynamic conditions in the specimen holder or a diameter of a specimen in the specimen holder. Embodiments of methods can promote endothelialization of a hybrid hemodialysis access graft or a hybrid femoral artery bypass graft by placing the hybrid hemodialysis access graft or the hybrid femoral artery bypass graft in a system embodiment according to the invention under conditions effective to promote endothelial cells to form a confluent monolayer on the surface of the hybrid graft.
    Type: Application
    Filed: January 24, 2008
    Publication date: October 23, 2008
    Inventor: Michael Dancu
  • Patent number: 7429377
    Abstract: A pharmaceutical composition having thrombolytic, anti-inflammatory and cytoprotective properties is described. The composition contains active proteases attached to a gel or to a mixture of a gel with a water-soluble polymer, said attachment being achieved with the help of radiation, preferably with the help of gamma-radiation or a flow of accelerated electrons. As the proteases the composition contains proteases which are stable at a temperature of up to 70° C., preferably at 30-40° C., for instance such as subtilysine, trypsin, chemotrypsin, papain or streptokinase. The gel is preferably polyethylene glycol gel, dextran gel, or polyglycane gel, and the water-soluble polymer is polyethylene glycol, polyvinyl alcohol, dextran or polyglycane. The method of preparing said composition and its use are also disclosed. The composition has a broad range of application, and can be used in cardiology, nephrology, surgery, rheumatology, gynecology and gastroenterology.
    Type: Grant
    Filed: December 24, 2002
    Date of Patent: September 30, 2008
    Assignee: Zakrytoe Aktsionernoe Obschestvo “Aksis”
    Inventors: Andrei Vladimirovich Artamonov, Evgeny Ivanovich Vereschagin, Oleg Vitalievich Grishin, Alexandr Vasilievich Troitsky
  • Patent number: RE40555
    Abstract: Biological agents such as secretory cells are encapsulated in a hydrophilic gel made of agarose or collagen-agarose and gelatin sponge-agarose combinations. In a preferred embodiment, semi-solid beads are formed from a suspension containing collagen, agarose and secretory cells such as pancreatic islets, the collagen is polymerized to form solid, agarose-collagen beads and the solid beads are coated with agarose. Coating is preferably by rolling the solid beads in about 5-10% agarose, contacting the rolled beads with mineral oil and washing oil from the beads. Beads containing secretory cells can be transplanted into a mammal to treat a condition caused by impaired secretory cell function.
    Type: Grant
    Filed: November 1, 2004
    Date of Patent: October 28, 2008
    Assignee: The Rogosin Institute
    Inventors: Kanti Jain, Albert L. Rubin, Barry Smith