Enzyme Or Microbial Cell Is Entrapped Within The Carrier (e.g., Gel, Hollow Fibre) Patents (Class 435/182)
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Patent number: 7514249Abstract: The subject invention concerns a composite comprising an organic fluid-swellable, fibrous matrix, such as collagen, and a mineral phase, such as calcium carbonate or phosphate mineral phase, for use as a biomimetic of bone. In another aspect, the subject invention concerns a process for making a composite involving the inclusion of acidic polymers to a supersaturated mineralizing solution, in order to induce an amorphous liquid-phase precursor to the inorganic mineral, which is then absorbed (pulled by capillary action) into the organic matrix. Advantageously, once solidified, a high mineral content can be achieved, with the inorganic mineral crystals embedded within the collagen fibers (intrafibrillarly) and oriented such that they are aligned along the long axes of the fibers of the organic matrix, thereby closely mimicking the natural structure of bone.Type: GrantFiled: October 22, 2003Date of Patent: April 7, 2009Assignee: The University of Florida Research Foundation, Inc.Inventors: Laurie B. Gower, Matthew J. Olszta, Elliot P. Douglas, Sivakumar Munisamy, Donna L. Wheeler
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Publication number: 20090075354Abstract: The present invention relates generally to nanofiber structures designed to support, entrap, entangle, preserve, and/or retain one or more biological materials. More specifically, the present invention relates to nanofiber matrix structures made from at least two different types of nanofibers that are designed to support, entrap, entangle, preserve, and/or retain one or more biological materials.Type: ApplicationFiled: June 7, 2006Publication date: March 19, 2009Applicant: THE UNIVERSITY OF AKRONInventors: Darrell H. Reneker, Daniel J. Smith
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Publication number: 20090061499Abstract: A method for the industrial production of the biocatalysts with biologically active material in the form of immobilized enzymes or microorganisms which are immobilized into the polyvinyl alcohol gel, and their use based on the fact, that the active biological material, formed by the mixture of free native or pretreated (aggregated) enzyme catalyst, or production microorganism, or part of them, and the polyvinyl alcohol gel, is used for their industrial production, the mixture is gelated and shaped in a stream of drying air at the temperature of 800 C to 150 C, considering the extent of the biologically active material, at the biocatalyst geometrical ratio of the surface to the volume kept larger than 7 mm‘l, and consequently thus prepared biocatalysts can be cultivated or stored and then use in biotechnological processes in the conditions, which ensure given biotechnological process higher productivity, higher production and enzymatic stability, long-term and repeated usage or definable process control with cType: ApplicationFiled: March 6, 2007Publication date: March 5, 2009Applicant: LENTIKAT'S, A.S.Inventors: Radek Stloukal, Michal Rosenberg, Martin Rebros
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Patent number: 7482152Abstract: Devices for the microencapsulation of cells include a first chamber for containing a cell-solution suspension. A plate covers one end of the first chamber. The plate has a plurality of apertures. A second chamber is provided for receiving encapsulated cells. The second chamber is separated from the first chamber by the plate. Cells from the first chamber are encapsulated by passing through the apertures in the plate and into the second chamber when pressure is applied to the cell-solution suspension.Type: GrantFiled: January 7, 2005Date of Patent: January 27, 2009Inventor: Melur Ramasubramanian
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Publication number: 20090004718Abstract: Disclosed is an antioxidant fermenting microorganism agent and a polyurethane foam including the same. The antioxidant fermenting microorganism agent is used to generate a substance that is capable of reducing an amount of volatile organic compounds generated from the polyurethane foam and has an antimicrobial ability. The polyurethane foam containing the antioxidant fermenting microorganism according to the present invention can be used as the material for the interior of a vehicle to protect humans from noxious substances causing sick car syndrome.Type: ApplicationFiled: May 9, 2007Publication date: January 1, 2009Applicants: DYMOS INC., SEOUL NATIONAL UNIVERSITY INDUSTRY FOUNDATIONInventors: Taek Lim, Jae-Yong Ko, Eun-Ju Lee, In-Sung Kim, Chang-Hong Lee
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Patent number: 7462471Abstract: Polymeric materials are used to make a pliable, non-toxic, injectable porous template for vascular ingrowth. The pore size, usually between approximately 100 and 300 microns, allows vascular and connective tissue ingrowth throughout approximately 10 to 90% of the matrix following implantation, and the injection of cells uniformly throughout the implanted matrix without damage to the cells or patient. The introduced cells attach to the connective tissue within the matrix and are fed by the blood vessels. The preferred material for forming the matrix or support structure is a biocompatible synthetic polymer which degrades in a controlled manner by hydrolysis into harmless metabolites, for example, polyglycolic acid, polylactic acid, polyorthoester, polyanhydride, or copolymers thereof. The rate of tissue ingrowth increases as the porosity and/or the pore size of the implanted devices increases.Type: GrantFiled: February 10, 2004Date of Patent: December 9, 2008Assignees: Massachusetts Institute of Technology, Children's Medical Center CorporationInventors: Antonios G. Mikos, Joseph P. Vacanti, Robert S. Langer, Linda G. Griffith, Georgios Sarakinos
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Publication number: 20080274201Abstract: This invention provides novel methods for the formation of biocompatible membranes around biological materials using photopolymerization of water soluble molecules. The membranes can be used as a covering to encapsulate biological materials or biomedical devices, as a “glue” to cause more than one biological substance to adhere together, or as carriers for biologically active species. Several methods for forming these membranes are provided. Each of these methods utilizes a polymerization system containing water-soluble macromers, species, which are at once polymers and macromolecules capable of further polymerization. The macromers are polymerized using a photoinitiator (such as a dye), optionally a cocatalyst, optionally an accelerator, and radiation in the form of visible or long wavelength UV light. The reaction occurs either by suspension polymerization or by interfacial polymerization.Type: ApplicationFiled: July 11, 2008Publication date: November 6, 2008Applicant: Board of Regents, The University of Texas SystemInventors: Jeffrey A. Hubbell, Chandrashekhar P. Pathak, Amarpreet S. Sawhney, Neil P. Desai, Syed F.A. Hossainy
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Patent number: 7422900Abstract: A system and method are provided for forming a connective tissue construct, such as a tendon construct, in vitro. A substrate is provided with at least two anchors secured thereto in spaced relationship. Fibroblast cells are provided on the substrate in the absence of a synthetic matrix, where at least some of the cells are in contact with the anchors. The cells are cultured in vitro under conditions to allow the cells to self-organize and become confluent between the anchors, where the anchors are receptive to the cells and allow the cells to attach thereto while permitting the cells to detach from the substrate to form a three-dimensional connective tissue construct.Type: GrantFiled: June 24, 2003Date of Patent: September 9, 2008Assignee: The Regents of the University of MichiganInventors: Paul E. Kosnik, Robert G. Dennis, Sarah C. Calve, Ellen M. Arruda
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Patent number: 7422879Abstract: Porous films are provided which include a blend of a film forming polymer and a non-film forming material, the film having a network of pores or channels throughout the film. The porous polymer films are formed between 0° and 80° C., retain porosity at elevated temperatures and are non-friable. A process for preparing porous polymer films and their applications are disclosed.Type: GrantFiled: April 27, 2004Date of Patent: September 9, 2008Assignee: Rohm and Haas CompanyInventors: Matthew S. Gebhard, Patricia M. Lesko, Albert B. Brown, David H. Young
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Patent number: 7422737Abstract: The present invention provides cellular solid carriers comprising viable microorganisms capable of controlling plant pathogens. The cellular solid carriers are formed from water-soluble hydrocolloid beads dried under conditions which preserve their porosity, thereby allowing efficient release of microorganisms or diffusion of products derived from the microorganisms from the beads to the surrounding environment.Type: GrantFiled: September 5, 2003Date of Patent: September 9, 2008Assignee: Yissam Research Development Company of the Hebrew University of JerusalemInventors: Amos Nussinovitch, Ilan Chet, Cheinat Zohar Perz
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Patent number: 7416869Abstract: Aqueous enzyme delivery systems as well as aqueous cosmetics and personal care products were provided which comprise at least one stabile enzyme and a polyglycerol containing at least 15 carbons. In addition, stabile non-aqueous enzyme delivery systems which are dispersions of at least one enzyme in an non-aqueous viscous liquid as well as a methods for the treatment of human skin in recreational water applications by means of enzymes are provided.Type: GrantFiled: August 19, 2004Date of Patent: August 26, 2008Assignee: Lonza Ltd.Inventors: Jacob Guth, Vickie Lentner
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Patent number: 7402381Abstract: Aspects of the invention can provide a method capable of easily realizing immobilization with the optimum density derived from a concentration control and without phase separation in coadsorption of a number of molecules. The immobilization method can include the step of dissolving a plurality of molecules to be immobilized to a solid phase substrate with a solvent to obtain a solution of the plurality of molecules, and the step of incubating the solution and the solid phase substrate in touch therewith. Each of the molecules can include a solid phase substrate joint portion having a jointing property to the solid phase substrate, a functional portion having a specific function, and a linker portion positioned between the solid phase substrate joint portion and the functional portion.Type: GrantFiled: August 27, 2004Date of Patent: July 22, 2008Assignee: Seiko Epson CorporationInventors: Hiroshi Takiguchi, Hitoshi Fukushima
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Publication number: 20080145860Abstract: The invention features an encapsulated cell indicator system that includes (a) indicator cells having a signal-responsive element operably linked to a reporter gene; (b) encapsulating material; and (c) a permeable membrane. In this encapsulated cell indicator system, the indicator cells are encapsulated in the encapsulated material and the encapsulated material and the indicator cells are surrounded by the permeable membrane.Type: ApplicationFiled: November 19, 2007Publication date: June 19, 2008Inventors: Ike W. Lee, Rabia Ballica, Jeffrey D. Croissant
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Publication number: 20080124780Abstract: An immobilized catalytic system comprising a carrier layer containing a catalytic entity and a permeable screening layer for providing controlled access between the immobilizing catalytic entity and the surrounding environment and methods of making such systems are disclosed. The carrier layer includes the catalytic entity mixed with a neutral or anionic carrier polymer, which may or may not be cross-linked with a cross-linking agent. The screening layer over the carrier layer includes a matrix of a cationic polymer that is permeable to molecules processed by, produced by or acted upon by the catalytic entity but is not permeable to the catalytic entity itself. Any counter ion to the neutral or anionic carrier polymer cannot be the same as the cationic polymer of the screening layer, and any counter ion to the cationic polymer cannot be the same as the neutral or anionic carrier polymer.Type: ApplicationFiled: January 24, 2008Publication date: May 29, 2008Inventors: Mansoor M. Amiji, Ehab S. Taqieddin
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Patent number: 7378088Abstract: The instant invention provides a base material characterized by having a continuous three-dimensional honeycomb structure for regenerating an air sinus. The invention further provides methods for regenerating an air sinus using the base material. The base material has a composition and a structure that is similar to the wall and bone structure which allows it to becomes a scaffold of the regeneration of the mucosa tissue covering the surface of an air sinus.Type: GrantFiled: May 6, 2004Date of Patent: May 27, 2008Inventors: Shin-ichi Kanemaru, Tatsuo Nakamura, Hisayoshi Kojima, Yasuhiko Shimizu
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Publication number: 20080090276Abstract: The thermal stability of enzymes is improved by incorporating the enzyme in a matrix of a non-reducing sugar, such as trehalose. Heat-stable compositions of enzymes and sugar are formed by adding the enzymes to a sugar syrup and drying the mixture either by spreading it on a carrier or by spray drying. The enzyme compositions retain higher levels of activity after being subjected to heat in product-forming processes, such as steam pelleting of animal feed supplemented with the enzyme compositions.Type: ApplicationFiled: October 12, 2006Publication date: April 17, 2008Inventors: Stefaan M.O. Van Dyck, Bruno Coppens, Ingrid Somers, Clifford A. Adams
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Patent number: 7354750Abstract: The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.Type: GrantFiled: October 20, 2003Date of Patent: April 8, 2008Assignee: Promega CorporationInventors: Daniel J. Simpson, Tonny Johnson, John Shultz, Roderick G. Flemming, Rebecca Godat, Sanchayita Kar, Robin Hurst
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Patent number: 7338657Abstract: The present invention relates to injectable compositions comprising biocompatible, hydrophilic, non-toxic and substantially spherical microspheres associated with stem cells useful for tissue construction and generation. The invention also relates to methods of tissue construction and generation, for the treatment of various tissue damage and defects, using the injectable compositions.Type: GrantFiled: March 15, 2001Date of Patent: March 4, 2008Assignee: BioSphere Medical, Inc.Inventors: Jean-Marie Vogel, Egisto Boschetti
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Patent number: 7332330Abstract: A device and a method of maintaining vascularization near an implant, especially a bioartificial hemofilter. By associating cells that excrete angiogenic factors with such an implant, vascularization to the tissue surrounding the implant can be maintained. In a bioartificial hemofilter, this facilitates filtrate transport to a collection fiber for drainage from the body. The cells can be genetically engineered, for example using an adenovirus vector encoding for vascular endothelial growth factor. Myoblasts and myotubes may be used in one embodiment of the present invention.Type: GrantFiled: September 11, 2001Date of Patent: February 19, 2008Assignee: RenaMed Biologics, Inc.Inventors: H. David Humes, William H. Fissell, Deborah A. Buffington, Evangelos Tziampazis
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Patent number: 7323190Abstract: Cell storage and delivery systems and methods for storing and delivering viable cells to a mammal are disclosed. The cell storage and delivery systems include a biodegradable and/or bioabsorbable fibrous matrix physically associated with viable cells to contain and release the cells at a controlled rate. The biodegradable and/or bioabsorable matrix can be formed by electrospinning fibers of biodegradable and/or bioabsorbable fiberizable material. The methods include methods for storing viable cells and for delivering viable cells to a mammal using the cell storage and delivery system.Type: GrantFiled: August 17, 2004Date of Patent: January 29, 2008Assignee: The Research Foundation at State University of New YorkInventors: Benjamin Chu, Benjamin S. Hsiao, Michael Hadjiargyrou, Dufei Fang, Xinhua Zong, Kwangsok Kim
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Patent number: 7323323Abstract: The invention relates to developments in the treatment of diabetes in mammals. Particularly it relates to a method of preparing a xenotransplantable porcine islet preparation capable upon xenotransplantation of producing porcine insulin in an appropriate recipient mammal, the method including or comprising the steps of: (I) harvesting the pancreas of piglets at or near full term gestation, and (ii) extracting pancreatic ? islet cells from the harvested pancreas wherein the islets (at least at some stage in the performance of the method) are exposed to nicotinamide. Further, the invention relates to a method of encapsulation of a xenotransplantable porcine islet preparation, and transplantation of such a preparation, or a capsule containing such a preparation, into an appropriate recipient mammal.Type: GrantFiled: May 22, 2003Date of Patent: January 29, 2008Assignee: Diabell Pty Ltd.Inventors: Robert Bartlett Elliott, Riccardo Calafiore, Gusseppe Basta
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Patent number: 7304015Abstract: The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.Type: GrantFiled: August 11, 2005Date of Patent: December 4, 2007Assignee: Promega CorporationInventors: Daniel J. Simpson, Tonny Johnson, Roderick G. Flemming
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Patent number: 7304016Abstract: The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.Type: GrantFiled: May 19, 2006Date of Patent: December 4, 2007Assignee: Promega CorporationInventors: Daniel J. Simpson, Tonny M. Johnson, Roderick G. Flemming
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Patent number: 7300779Abstract: Granules that include a protein core are described. The protein core includes a protein matrix which includes a protein mixed together with a starch. The protein matrix can be layered over a seed particle or the protein core can be homogeneous. The protein can be an enzyme or a therapeutic protein.Type: GrantFiled: September 13, 2004Date of Patent: November 27, 2007Assignee: Genencor International, Inc.Inventors: Nathaniel T. Becker, Thomas S. Green
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Patent number: 7297331Abstract: Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.Type: GrantFiled: August 16, 2004Date of Patent: November 20, 2007Assignee: The Rogosin InstituteInventors: Shirin Asina, Kanti Jain, Albert L. Rubin, Barry Smith, Kurt Stenzel
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Publication number: 20070232514Abstract: The present invention relates to a liquid composition comprising an enzyme and an enzyme stabilizer of the following formula: or a salt thereof, wherein R1 is selected from the group consisting of C0-C5 alkyl, substituted C1-C5 alkyl, C1-C5 alkenyl and substituted C1-C6 akenyl and R2, R3, R4, R5 and R6 is selected from the group consisting of hydrogen, C1-C6 alkyl, substituted C1 to C6 alkyl, aryl, substituted aryl, hydroxyl, hydroxyl derivative, amine, C1-C6 alkylated amine, amine derivative, halogen, nitro, thiol, thiol derivative, aldehyde, acid, acid salt, ester, sulfonate or phosphonate.Type: ApplicationFiled: March 29, 2007Publication date: October 4, 2007Applicant: Novozymes A/SInventors: Lone Kierstein Nielsen, Ole Simonsen, Karl Werntoft, Niclas Ilestam
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Patent number: 7273736Abstract: The invention relates processes for producing enzyme containing granules having an enzyme containing core and a shell coating the core wherein the preparation of the core and application of the shell are independent and are physically separated by time and/or location.Type: GrantFiled: February 11, 2004Date of Patent: September 25, 2007Assignee: Novozymes A/SInventor: Poul Bach
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Patent number: 7256040Abstract: The invention concerns an apparatus for preparing monolayers of cells. The apparatus comprises a container (6) for a cryosubstitution system and an insert (1) for the container, the insert (1) having a surface (1a) and a plurality of orifices (3). An SCS (2) together with a cellular monolayer (20) is insertable into each of the orifices (3). The SCS (2) is thus arranged perpendicular to the surface (1a) of the insert (1).Type: GrantFiled: June 14, 2002Date of Patent: August 14, 2007Assignee: Leica Mikrosysteme GmbHInventor: Heinz Horstmann
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Patent number: 7247462Abstract: An enzymatic catalyst in the granular form which is non-dispersible in water comprising an inner core provided with a coating which coating comprises an enzymatic material characterised in that the coating has a thickness of from 30 to 400 microns and the ratio of the size of the core to the thickness of the coating is from 2 to 20. A method for the preparation of the catalyst and the use of the catalyst in the conversion of 2-hydroxy-4-methylthio butyronitrile to ammonium 2-hydroxy-4-methylthio butanoate is also claimed.Type: GrantFiled: June 22, 2001Date of Patent: July 24, 2007Assignee: Adisseo Ireland LtdInventors: Olivier Favre-Bulle, Jean-Claude Le Thiesse
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Patent number: 7247314Abstract: A biocompatible material genus serves as the foundation for multiple material composition species, each adapted to a specific therapeutic indication.Type: GrantFiled: December 2, 2004Date of Patent: July 24, 2007Assignee: Neomend, IncInventors: Olexander Hnojewyj, Charles F. Milo, Gregory M Cruise
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Patent number: 7244442Abstract: Polyhydroxyalkanoates (PHAs) from which pyrogen has been removed are provided for use in numerous biomedical applications. PHAs which have been chemically modified to enhance physical and/or chemical properties, for targeting or to modify biodegradability or clearance by the reticuloendothelial system (RES), are described. Methods for depyrogenating PHA polymers prepared by bacterial fermentation processes are also provided, wherein pyrogens are removed from the polymers without adversely impacting the polymers' inherent chemical structures and physical properties. PHAs with advantageous processing characteristics, including low melting points and/or solubility in non-toxic solvents, are also described. PHAs are provided which are suitable for use in in vivo applications such as in tissue coatings, stents, sutures, tubing, bone and other prostheses, bone or tissue cements, tissue regeneration devices, wound dressings, drug delivery, and for diagnostic and prophylactic uses.Type: GrantFiled: August 15, 2003Date of Patent: July 17, 2007Assignee: Metabolix, Inc.Inventors: Simon F. Williams, David P. Martin, Tillman Gerngross, Daniel M. Horowitz
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Patent number: 7244598Abstract: Array systems that facilitate the simultaneous monitoring of many interactions between biological molecules and the analysis of cellular protein interactions with high throughput. The present invention provides methods and arrays for analyzing biochemical pathways by forming an array of immobilized biomolecules; exposing the array to biomolecules in solution; and detecting modification of the immobilized biomolecules, modification of the biomolecules in solution, and/or binding of biomolecules in solution to immobilized biomolecules.Type: GrantFiled: August 14, 2001Date of Patent: July 17, 2007Assignee: Surface Logix, Inc.Inventor: David Duffy
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Patent number: 7229808Abstract: The present invention relates to a process for producing a bioreactor using viable immobilized biological material and to the bioreactor itself. More particularly, the present invention relates to a process for producing a siliceous layer capable of biomass immobilization and selectively cutting off macromolecules having a molecular weight higher than a selected threshold, comprising the steps of: a) supplying a gas flow of a gas carrier saturated by a mixture of silicon alkoxides selected from the group comprising (1)Si(OR)4, (2)SiH(OR)3, (3)R?Si(OR)3 and (4)R?SiH(OR)2 wherein R and R?, equal or different each other, are alkyl and/or aryl groups, wherein said gas flow is prepared by bubbling the gas carrier into a liquid mixture of said alkoxides in the ratio of (1)40–85/(2)0–60/(3)0–60/(4)0–60 (% v/v), preferably in the ratio of (1)40–85/(2)0–50/(3)0–50/(4)0–50 (% v/v), more preferably in the ratio of (1)50–80/(2)0–20/(3)5–30/(4)5–30 (% v/v), at a temperature of from 20 to 180° C.Type: GrantFiled: January 18, 2002Date of Patent: June 12, 2007Assignee: Silbiotec Due S.A.Inventors: Gianni Carturan, Roberto Dal Toso, Renzo Dal Monte
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Patent number: 7211249Abstract: The present invention provides a method of sustaining the rate of heat felt on the skin as a result of an exothermic reaction between a combination of an unencapsulated and an encapsulated catalase, and peroxide. The catalase component provides a sustained heat release and in combination with other non-catalase heat-generating agents, the rate of heat released and felt on the skin can be further controlled. The compositions of the present invention also soften the skin and contribute to the health of the skin by reducing the presence of oxygen free radicals on the skin surface.Type: GrantFiled: March 17, 2003Date of Patent: May 1, 2007Assignee: Color Access, Inc.Inventors: Steven F. Schnittger, Denise M. DiCanio, Peter J. Lentini
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Patent number: 7192765Abstract: Provided are a nitrite-type nitrification carrier and a method for producing the same and a method and an apparatus for removing nitrogen using the same, in which the quantity of organic matter to be added can be substantially reduced to reduce running cost. A method for producing a nitrite-type nitrification carrier in which ammonia-oxidizing bacteria for nitrifying ammonium to nitrite is preferentially accumulated comprises the steps of: entrapping and immobilizing any sludge selected from sediment from a lake, a river or the sea, soil from the surface of the earth, or activated sludge from a sewage-treatment plant into a monomer or a prepolymer for immobilizing microorganisms; and then subjecting the entrapped and immobilized sludge to heat treatment at 30 to 80° C.Type: GrantFiled: January 22, 2003Date of Patent: March 20, 2007Assignee: Hitachi Plant Engineering & Construction Co., Ltd.Inventors: Tatsuo Sumino, Kazuichi Isaka
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Patent number: 7166451Abstract: A multilayer enzyme immobilization process is provided comprising adsorbing a polyethyleneimine (PEI) solution in a fibrous matrix, and adding an enzyme to the fibrous matrix, which comprises a plurality of fibrils. The process further comprises forming at least two layers of PEI-enzyme aggregates on the fibrils, and cross-linking the multilayer PEI-enzyme aggregates. The process can further comprise washing the fibrils containing the cross-linked PEI-enzyme aggregates with distilled water and acetic acid buffer subsequent to cross-linking. However, the PEI-containing matrix is not washed prior to the addition of enzyme. The enzyme can be ?-galactosidase and the fibrous matrix can be cotton cloth. The multilayer immobilized enzyme can be employed in a biocatalyst reactor for production of galacto-oligosaccharides from lactose and the hydrolysis of lactose to glucose and galactose.Type: GrantFiled: February 24, 2004Date of Patent: January 23, 2007Assignee: The Ohio State UniversityInventors: Shang-Tian Yang, Nedim Albayrak
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Patent number: 7160716Abstract: A device for inducing an immune response in cancer therapy in which isolated living malignant tissue is enclosed in a space formed by a microporous membrane and a holder which is introduced into a body cavity so that macromolecular and cellular immune system components can pass freely in both directions through the membrane while malignant cells of the tumor tissue remain trapped inside.Type: GrantFiled: May 9, 2003Date of Patent: January 9, 2007Inventors: Vladimir Kalina, Pavel Richter, Vratislav Horak
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Patent number: 7153519Abstract: Water soluble macromers are modified by addition of free radical polymerizable groups, such as those containing a carbon-carbon double or triple bond, which can be polymerized under mild conditions to encapsulate tissues, cells, or biologically active materials. The polymeric materials are particularly useful as tissue adhesives, coatings for tissue lumens including blood vessels, coatings for cells such as islets of Langerhans, coatings, plugs, supports or substrates for contact with biological materials such as the body, and as drug delivery devices for biologically active molecules.Type: GrantFiled: June 25, 2003Date of Patent: December 26, 2006Assignee: Board of Regents, The University of Texas SystemInventors: Jeffrey A. Hubbell, Chandrashekhar P. Pathak, Amarpreet S. Sawhney, Neil P. Desai, Jennifer L. Hill, Syed F. A. Hossainy
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Patent number: 7128931Abstract: The present invention relates to semi-permeable microcapsules, which exhibit an increased resistance to mechanical stresses as well as an increased resistance to chemical degradation. More specifically, this semi-permeable microcapsule has a bead enclosing a material such as living cells, covalently linked to a semi-permeable layer, made of a polycation cross-linking derivative, covering the bead. The present invention is also directed to a pharmaceutical composition comprising the above-mentioned semi-permeable microcapsules. The present invention is also directed to the use of such microcapsule in a method for microencapsulating a beaded material and in a method for treating diabetes in a subject.Type: GrantFiled: October 17, 2003Date of Patent: October 31, 2006Inventors: Francois Leblond, Jean-Pierre Halle
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Patent number: 7118900Abstract: The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies of polymer-microparticle composites including the AC electric field-mediated assembly of patterned, self-supporting organic (polymeric) films and organic-polymer-microparticle composites of tailored composition and morphology. The present invention further relates to the incorporation of said assemblies into other structures. The present invention also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, and micro-reactors, and miniaturized format for generation of multifunctional thin films. This invention also provides simple methods and apparatus for synthesizing thin films of tailored composition and morphology.Type: GrantFiled: June 21, 2002Date of Patent: October 10, 2006Assignee: BioArray Solutions Ltd.Inventors: Michael Seul, Sukanta Banerjee, Kairali Podual, Ye Hong
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Patent number: 7112315Abstract: Unique single walled nanotube compositions and methods for making the composition wherein the nanotubes encloses foreign material capable of forming thread-like material having a solid form at ambient temperatures and pressures.Type: GrantFiled: July 15, 1999Date of Patent: September 26, 2006Assignee: The Regents of the University of CaliforniaInventor: Ching-Hwa Kiang
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Patent number: 7112453Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.Type: GrantFiled: August 5, 2002Date of Patent: September 26, 2006Assignee: Ciphergen Biosystems, Inc.Inventors: T. William Hutchens, Tai-Tung Yip
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Patent number: 7105339Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.Type: GrantFiled: July 23, 2003Date of Patent: September 12, 2006Assignee: Ciphergen Biosystems, Inc.Inventors: T. William Hutchens, Tai-Tung Yip
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Patent number: 7105337Abstract: Biological treatment for an environmental hormone in water can be efficiently performed by use of a microorganism-immobilized carrier is provided. To biologically remove an environmental hormonal substance in water by a microorganism-immobilized carrier having microorganism immobilized onto a carrier. The microorganism-immobilized carrier is formed of an immobilizing material prepared by mixing and polymerizing a hydrophilic prepolymer and a hydrophobic prepolymer or prepared by polymerizing a prepolymer having a hydrophilic group and a hydrophobic group in a molecule.Type: GrantFiled: January 18, 2002Date of Patent: September 12, 2006Assignee: Hitachi Plant Engineering & Construction Co., Ltd.Inventors: Kazuichi Isaka, Tatsuo Sumino
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Patent number: 7056726Abstract: The invention concerns a semipermeable chamber for encapsulation of cells producing at least a biological substance of interest, characterised in that it consists of a porous polycarbonate biocompatible film modified at the surface by generated polar sites and covered with at least a layer of hydrophilic polymer.Type: GrantFiled: January 29, 2002Date of Patent: June 6, 2006Assignees: Association pour les Transferts de Technologies du Mans, Universite Catholique de louvain, Centre Europeen d'Etude du Diabete (CEED)Inventors: Gilbert Legeay, Patrick Bertrand, Alain Belcourt, Laurence Kessler
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Patent number: 7041504Abstract: Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.Type: GrantFiled: December 12, 2000Date of Patent: May 9, 2006Assignee: The Rogosin InstituteInventors: Shirin Asina, Kanti Jain, Albert L. Rubin, Barry Smith, Kurt Stenzel
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Patent number: 7026146Abstract: A method for maintaining the viability and subsequent activity of microorganisms utilized in a variety of environments to promote biodecontamination of surfaces. One application involves the decontamination of concrete surfaces. Encapsulation of microbial influenced degradation (MID) microorganisms has shown that MID activity is effectively maintained under passive conditions, that is, without manual addition of moisture or nutrients, for an extended period of time.Type: GrantFiled: August 12, 2002Date of Patent: April 11, 2006Assignee: Battelle Energy Alliance, LLCInventors: Robert D. Rogers, Melinda A. Hamilton, Lee O. Nelson, Jennifer Benson, Martin J. Green, Timothy N. Milner
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Patent number: 7020560Abstract: A method for cell-based combinatorial logic includes the steps of providing at least one genetically engineered cell, the genetically engineered cell having at least one transcriptional unit. The transcriptional unit includes a gene and a promoter, wherein application of a stimulus to the promoter results in the expression of a gene product. An energetic or chemical stimulus is applied to activate the promoter, wherein the detection of an output signal corresponds to the presence of a gene product. The cell can include a plurality of transcriptional units configured to form logic gates. The logic gates of a plurality of cells can be operably interconnected by release of output signals, such as chemical stimuli.Type: GrantFiled: September 6, 2001Date of Patent: March 28, 2006Assignee: University of Tennessee Research FoundationInventors: Gary S. Sayler, James T. Fleming, Bruce Applegate, Michael L. Simpson
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Patent number: 7018820Abstract: Disclosed is a method of removing residual enzymes when microencapsulating enzymes, and more particularly, for inactivating enzymes remaining in an uncapsulated form during microencapsulation of enzymes by treating dispersion fluid of microcapsules containing enzymes with ozone, together with removal of microorganisms harmful to human beings, where the ozone treatment is conducted for 1–10 min with 1–10 ppm of ozone generated from a UV lamp in a range from 150 to 200 nm wavelength.Type: GrantFiled: July 17, 2002Date of Patent: March 28, 2006Inventors: Hae-Soo Kwak, Joon Bum Lee
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Patent number: RE40555Abstract: Biological agents such as secretory cells are encapsulated in a hydrophilic gel made of agarose or collagen-agarose and gelatin sponge-agarose combinations. In a preferred embodiment, semi-solid beads are formed from a suspension containing collagen, agarose and secretory cells such as pancreatic islets, the collagen is polymerized to form solid, agarose-collagen beads and the solid beads are coated with agarose. Coating is preferably by rolling the solid beads in about 5-10% agarose, contacting the rolled beads with mineral oil and washing oil from the beads. Beads containing secretory cells can be transplanted into a mammal to treat a condition caused by impaired secretory cell function.Type: GrantFiled: November 1, 2004Date of Patent: October 28, 2008Assignee: The Rogosin InstituteInventors: Kanti Jain, Albert L. Rubin, Barry Smith