Oxidoreductase (1. ) (e.g., Luciferase) Patents (Class 435/189)
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Patent number: 8916365Abstract: Transgenic Yarrowia species are disclosed herein that comprise a polynucleotide encoding a cytosolic malic enzyme, a lipid content that is at least about 35% by weight of the dry cell weight of the Yarrowia species, and an engineered polyunsaturated fatty acid (PUFA) biosynthetic pathway, wherein overexpression of the cytosolic malic enzyme increases lipid content.Type: GrantFiled: March 14, 2013Date of Patent: December 23, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Daniel Joseph Macool, Zhixiong Xue, Quinn Qun Zhu
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Patent number: 8911981Abstract: Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.Type: GrantFiled: March 15, 2013Date of Patent: December 16, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
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Patent number: 8911982Abstract: The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids.Type: GrantFiled: October 19, 2010Date of Patent: December 16, 2014Assignee: Evonik Degussa GmbHInventors: Steffen Schaffer, Mirja Wessel, Anja Thiessenhusen
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Publication number: 20140363860Abstract: The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially ?3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.Type: ApplicationFiled: July 4, 2014Publication date: December 11, 2014Applicant: University of JiangnanInventors: Haiqin Chen, Zhennan Gu, Hao Zhang, Wei Chen, Yuanda Song, Fengwei Tian, Jianxin Zhao, Yongquan Chen
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Publication number: 20140363874Abstract: A method for producing laccase by liquid fermentation of Lentinus edodes comprises the following steps of: (1) inoculating strains of Lentinus edodes into the PD liquid fermentation medium to perform activation culture and obtain a seed liquid; (2) inoculating the seed solution into a liquid fermentation medium to perform liquid fermentation culture, then adding an expansin solution to perform further culture, separating the supernatant to obtain a ferment solution; (3) extracting laccase from the ferment solution. A plant expansin and an optimized fermentation method are applied for producing laccase by liquid fermentation of Lentinus edodes, which increases the liquid fermentation output of laccase of Lentinus edodes remarkably, by more than 3 times in comparing with traditional fermentation production methods.Type: ApplicationFiled: March 22, 2013Publication date: December 11, 2014Applicant: INSTITUTE OF AGRICULTURAL RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRICULTURAL SCIENCESInventors: Qiang Yao, Zhiyuan Gong, Zhaohui Liu, Jiandong Han, Qi Wang, Xiao Liu, Luchang Wan, Pengfei Ren, Haixia Ren, Jin Li, Peng Yang, Tao Sun
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Patent number: 8906667Abstract: A method of increasing cellular NADPH levels by expressing one or more genes that encode an enzyme that causes the production of NADPH. The system is combined with other enzymes that require NADPH, thus improving the overall yield of the desired product.Type: GrantFiled: August 29, 2007Date of Patent: December 9, 2014Assignee: William Marsh Rice UniversityInventors: Ka-Yiu San, George Bennett, Henry Lin, Irene Martinez, Jiangfeng Zhu
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Publication number: 20140356389Abstract: Disclosed herein are various open reading frames from a strain of E. coli responsible for neonatal meningitis (MNEC), and a subset of these that is of particular interest for preparing compositions for immunising against MNEC infections.Type: ApplicationFiled: June 2, 2014Publication date: December 4, 2014Applicants: J. CRAIG VENTER INSTITUTE, INC., NOVARTIS VACCINES AND DIAGNOSTICS SRLInventors: Vega MASIGNANI, Danilo Gomes MORIEL, Francesco BERLANDA SCORZA, Nathalie NORAIS, Maria Rita FONTANA, Mariagrazia PIZZA, Laura SERINO, Herve TETTELIN
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Patent number: 8900844Abstract: A modified pyrroloquinoline quinone glucose dehydrogenase that exhibits a high selectivity for glucose is provided. A modified pyrroloquinoline quinone glucose dehydrogenase is disclosed in which the amino acid residue G at Position 99 of a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 1, or the amino acid residue G at Position 100 of the pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 3, is substituted by the amino acid sequence TGZN (where Z is SX, S, or N and X is any amino acid residue). The modified PQQGDH of the present invention may additionally comprise one or more mutations selected from the group consisting of Q192G, Q192A, or Q192S; L193X; E277X; A318X; Y367A, Y367F, or Y367W; G451C; and N452X (where X is any amino acid residue).Type: GrantFiled: October 25, 2012Date of Patent: December 2, 2014Assignees: ARKRAY, Inc., Ultizyme International Ltd.Inventor: Koji Sode
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Publication number: 20140349338Abstract: The present invention relates to novel bacteria and the uses thereof. The invention particularly relates to bacteria having a metabolic pathway ratio between Pentose phosphate and glycolysis greater than 0.5, and their uses in the chemical, pharmaceutical or agro-chemical industries, e.g., for producing compounds of industrial interest.Type: ApplicationFiled: December 21, 2012Publication date: November 27, 2014Applicant: DEINOVEInventors: Jean-Paul Leonetti, Juha-Pekka Pitkanen, Paula Jouhten
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Patent number: 8895808Abstract: Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.Type: GrantFiled: December 14, 2012Date of Patent: November 25, 2014Assignee: Monsanto Technology LLCInventors: Larry A. Gilbertson, Susan J. Johnson
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Patent number: 8895307Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.Type: GrantFiled: July 16, 2012Date of Patent: November 25, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
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Patent number: 8895271Abstract: The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: December 8, 2010Date of Patent: November 25, 2014Assignee: Codexis, Inc.Inventors: Yong Koy Bong, Michael D. Clay, Steven J. Collier, Benjamin Mijts, Michael Vogel, Xiyun Zhang, Jun Zhu, Jovana Nazor, Derek Smith, Shiwei Song
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Publication number: 20140344997Abstract: Disclosed are polynucleotides encoding polypeptides that comprise the biosynthetic pathway for lignin in the jute plant. The present invention relates generally to the field of plant lignin biosynthesis genes, polypeptides encoded by such genes, and the use of such polynucleotide and polypeptide sequences for controlling plant lignin production. Also disclosed are methods for using the polynucleotides and polypeptides to influence the quality and amount of fiber produced by jute.Type: ApplicationFiled: April 25, 2012Publication date: November 20, 2014Applicant: Bangladesh Jute Research InstituteInventors: Maqsudul Alam, Haseena Khan, Mahboob Zaman, Mohammed K. Uddin, Mohammed S. Haque, Mohammed S. Islam, Muhammed S. Azam
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Publication number: 20140335155Abstract: The present invention provides a composition for improving skin conditions, containing the extracellular domain of human bone morphogenetic protein receptor 1a as an active ingredient. The present invention contains the extracellular domain of human bone morphogenetic protein receptor 1a as an active ingredient inside a nanoliposome comprising hydrogenated lecithin as a constituent component, and the particle size is 50-250 nm. The composition of the present invention reduces wrinkles and alleviates atopic dermatitis. In addition, the present invention provides a method for improving skin conditions, comprising the step of administering a composition containing the extracellular domain of human bone morphogenetic protein receptor 1a as an active ingredient to a subject.Type: ApplicationFiled: August 30, 2012Publication date: November 13, 2014Applicant: JOINT CENTER FOR BIOSCEINCESInventors: Zung Yoon Yang, Byung Hak Yoon, Seung-Hyon Choi
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Publication number: 20140335553Abstract: Methods are provided measuring L-lysine using a variant enzyme, an L-lysine measurement kit, and an enzyme sensor. Variant L-amino acid oxidase having a predetermined amino acid mutation, and having oxidase activity that is highly substrate-specific for L-lysine; a method for measuring L-lysine using this variant enzyme; an L-lysine measurement kit; and an enzyme sensor are also provided.Type: ApplicationFiled: July 29, 2014Publication date: November 13, 2014Applicants: AJINOMOTO CO., INC., TOYAMA PREFECTUREInventors: Yasuhisa Asano, Daisuke Matsui
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Publication number: 20140335579Abstract: The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerin and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: ApplicationFiled: July 23, 2014Publication date: November 13, 2014Inventors: Toralf Senger, Jörg Bauer, Johnathan A. Napier
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Patent number: 8883142Abstract: A reagent measuring a glycated protein, comprising a protease and a protein of any one of [1] to [4] below: [1] a protein comprising the amino acid sequence represented by SEQ ID NO:1; [2] a protein comprising an amino acid sequence with one to ten amino acid deletions, substitutions, or additions in the amino acid sequence represented by SEQ ID NO:1, and having fructosyl peptide oxidase activity; [3] a protein comprising an amino acid sequence having 99% or higher homology to the amino acid sequence represented by SEQ ID NO:1, and having fructosyl peptide oxidase activity; and [4] a protein having fructosyl peptide oxidase activity, which is encoded by an expression plasmid harbored by the Escherichia coli XL1-Blue MRF? strain deposited under Accession No. FERM BP-11026.Type: GrantFiled: May 1, 2014Date of Patent: November 11, 2014Assignee: Kyowa Medex Co., Ltd.Inventors: Kazuo Aisaka, Keiko Suzuki
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Patent number: 8883467Abstract: The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.Type: GrantFiled: September 27, 2013Date of Patent: November 11, 2014Assignee: Codexis, Inc.Inventors: Robert McDaniel, Behnaz Behrouzian, Louis Clark, Douglas Hattendorf, Fernando Valle
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Patent number: 8883475Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.Type: GrantFiled: October 23, 2012Date of Patent: November 11, 2014Assignee: Codexis, Inc.Inventors: Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
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Patent number: 8883442Abstract: There are disclosed a culture media and culture supplements for cultivating microorganisms, including Listeria spp and methods for culturing microorganisms including Listeria spp. There are also disclosed methods for detecting the presence of microorganisms including Listeria spp in samples.Type: GrantFiled: July 11, 2012Date of Patent: November 11, 2014Assignee: Foodchek Systems, Inc.Inventors: Gabriela Martinez, David Claveau, Lila Maduro
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Publication number: 20140328821Abstract: The present invention relates to nucleic acids coding for and capable of expressing a rod-derived cone viability factor (RdCVF) and viral vectors containing these nucleic acids. The invention also relates to compositions and pharmaceutical preparations comprising these nucleic acids or vectors, methods of producing or secreting an RdCVF, and methods of treatment.Type: ApplicationFiled: October 26, 2012Publication date: November 6, 2014Applicant: Wellstat Ophthalmics CorporationInventor: Tianci Luo
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Patent number: 8877476Abstract: A soluble and stable form of 5-lipoxygenase (5-LOX) has been made, 5-Lox is the enzyme which initiates leukotriene biosynthesis by catalyzing the two-step transformation of arachidomc acid to leukotriene A4 (LTA4). The soluble and stable 5-LOX is suitable for a number of applications, including, but not limited to, high throughput screening of 5-LOX inhibitors, structural analysis of the enzyme's active site, designing inhibitors based on the three-dimensional structure of the enzyme's active site, and synthesis of LTA4. Using Stable-5-LOX, the crystal structure for 5-LOX has been resolved and the amino acids defining the active site determined.Type: GrantFiled: May 31, 2011Date of Patent: November 4, 2014Assignee: Board of Supervisors of Louisiana State University and Agricultural and Mechanical CollegeInventors: Marcia E. Newcomer, Sue G. Bartlett, Nathaniel C. Gilbert
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Patent number: 8877475Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: GrantFiled: September 11, 2012Date of Patent: November 4, 2014Assignee: Codexis, Inc.Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
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Publication number: 20140322792Abstract: Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.Type: ApplicationFiled: May 10, 2014Publication date: October 30, 2014Applicant: The California Institute of TechnologyInventors: Edgardo Farinas, Frances H. Arnold, Ulrich Schwaneberg, Anton Glieder
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Publication number: 20140322388Abstract: The invention is directed to the enhanced expression and purification of lipoxygenase enzymes. These enzymes are of wide-spread industrial importance, often produced in heterologous microbial systems. Preferably, the lipoxygenase produced by the methods of the invention is a plant-derived enzyme and expressed at high-levels in a microbial system that includes a protease-deficient host and one or more chaperone expression plasmids. The invention is also directed to amino acid and nucleic acid fragments of the lipoxygenase enzyme including fragments in expression constructs encoding all or a portion of one or more lipoxygenase genes. The invention is also directed to methods of manufacturing bread and other food and also non-food products with lipoxygenase manufactured by the methods of the invention.Type: ApplicationFiled: April 28, 2014Publication date: October 30, 2014Applicant: US NewWin, Inc.Inventors: Gaofeng Liu, Min-Ju Chang, Averell L. Gnatt, Tian Ye
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Patent number: 8870980Abstract: The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.Type: GrantFiled: December 21, 2011Date of Patent: October 28, 2014Assignee: Neste Oil OyjInventors: Annika Malm, Simo Laakso, Ossi Pastinen, Heidi Kahelin, Miia Mujunen
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Publication number: 20140314727Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.Type: ApplicationFiled: July 3, 2014Publication date: October 23, 2014Applicant: Commonwealth Scientific and Industrial Research OrganisationInventors: Surinder Pal Singh, Stanley Suresh Robert, Peter David Nichols, Susan Irene Ellis Blackburn, Xue-Rong Zhou, James Robertson Petrie, Allan Graham Green
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Publication number: 20140315313Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.Type: ApplicationFiled: April 20, 2012Publication date: October 23, 2014Applicant: DANISCO US INC.Inventors: Elizabeth A. Bodie, Robert James Pratt, II
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Publication number: 20140308731Abstract: Two novel cytochrome P450 genes are isolated from sorghum, each gene encoding a protein having pentadecatrienyl resorcinol hydroxylase activity. Expression vectors containing these sequences are made and used to elevate levels of pentadecatrienyl resorcinol hydroxylase in transgenic cells and organisms.Type: ApplicationFiled: April 15, 2013Publication date: October 16, 2014Inventor: The United States of America, as Represented by the Secretary of Agriculture
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Publication number: 20140308262Abstract: The invention provides fusion protein constructs comprising a functional mitochondrial protein and methods of treating mitochondrial disorders by the fusion proteins and compositions thereof.Type: ApplicationFiled: September 23, 2013Publication date: October 16, 2014Inventor: Haya Lorberboum-Galski
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Publication number: 20140308732Abstract: The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ?-chloroalcohols from ?-chloroketones.Type: ApplicationFiled: June 24, 2014Publication date: October 16, 2014Inventors: Yong Koy Bong, Michael Vogel, Steven J. Collier, Vesna Mitchell, Jagadeesh Mavinahalli
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Publication number: 20140308716Abstract: Provided is a novel method for preparing metabolites of atorvastatin using bacterial cytochrome P450, and a composition therefor, and more particularly, a composition for preparing 2-hydroxylated product of 4-hydroxylated product from atorvastatin including bacterial cytochrome P 450 BM3 (CYP102A1), CYP102A1 mutants, and chimeras derived from the CYP102A1 mutants, a kit therefor, and a method for preparing thereof.Type: ApplicationFiled: September 27, 2012Publication date: October 16, 2014Inventors: Chul-Ho Yun, Dong-Hyun Kim, Ji-Yeon Kang, Sun-Ha Park, Sang-Hoon Ryu
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Publication number: 20140301945Abstract: Provided are novel human copper-zinc superoxide dismutase, also known as superoxide dismutase 1 or SOD1, specific antibodies as well as fragments, derivatives and variants thereof as well as methods related thereto. Assays, kits, and solid supports related to antibodies specific for SOD1 are also disclosed. The antibody, immunoglobulin chain(s), as well as binding fragments, derivatives and variants thereof can be used in pharmaceutical and diagnostic compositions for SOD1 targeted immunotherapy and diagnosis, respectively.Type: ApplicationFiled: December 19, 2011Publication date: October 9, 2014Applicants: UNIVERSITY OF ZURICH, NEURIMMUNE HOLDING AGInventors: Fabio Montrasio, Maria Grazia Barenco Montrasio, Jan Grimm, Roger Nitsch, Christoph Hock, Tobias Welt, Jordan McAfoose, Marcel Maier
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Publication number: 20140303035Abstract: The present invention provides nucleic acid constructs that encode fusion peptides comprising a bioluminescent protein and a precursor of a secreted peptide or protein expressed at the cell surface and high throughput screening assays using same.Type: ApplicationFiled: November 7, 2012Publication date: October 9, 2014Inventors: Sean Burns, David Altshuler, Amedeo Vetere
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Publication number: 20140302567Abstract: Embodiments of the present disclosure include methods of treating a biomass, in particular lignocellulosic biomass (e.g., switch grass, sweet sorghum, miscanthus, pine wood, corn stover, and the like), under appropriate conditions to effectively reduce and/or reform the lignin contents in lignocellulosic biomass without significantly reducing its sugar contents.Type: ApplicationFiled: October 11, 2012Publication date: October 9, 2014Applicant: THE UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INCInventors: Qingguo Huang, Sudeep S. Sidhu
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Publication number: 20140302583Abstract: DNA constructs as well as methods for the production of unicellular organisms capable of producing hydrogen peroxide resistance proteins are disclosed. DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular organisms for the expression of hydrogen peroxide production proteins are also disclosed. In addition, DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular organisms for the expression of hydrogen peroxide resistance and hydrogen peroxide production proteins are disclosed.Type: ApplicationFiled: October 4, 2012Publication date: October 9, 2014Inventors: Stephen K. Herbert, Levi G. Lowder
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Patent number: 8853491Abstract: Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different luciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a biolmuniescent reaction is desired. Phototransformation may be utilized to vary the wavelength of light emitted from the mature plant.Type: GrantFiled: June 2, 2011Date of Patent: October 7, 2014Inventor: Bruce Eric Hudkins
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Patent number: 8852909Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: August 19, 2013Date of Patent: October 7, 2014Assignee: Codexis, Inc.Inventors: Jack Liang, Stephane J. Jenne, Emily Mundorff, Charlene Ching, John M. Gruber, Anke Krebber, Gjalt W. Huisman
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Publication number: 20140295522Abstract: The intention is to improve storage stability in a liquid washing or cleaning agent which contains a protease and amylase. This is achieved by using a protease which comprises an amino acid sequence which, over the entire length thereof, is at least 70% identical to the amino acid sequence stated in SEQ ID no. 1 and, in the numbering according to SEQ ID no. 1, has the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions which are selected from the group consisting of S3T, V4I and V199I.Type: ApplicationFiled: June 12, 2014Publication date: October 2, 2014Inventors: Nina Mussmann, Thomas Eiting, Thorsten Bastigkeit, Konstantin Benda, Hendrik Hellmuth
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Publication number: 20140294842Abstract: A novel class of hydroxylases is described having the amino acid sequence of SEQ ID No. 2, 4, 6 and 8, and variants and fragments thereof having HIF hydroxylation activity. The polypeptides of the invention have in particular prolyl hydroxylase activity. An assay method monitors the interaction of the HIF hydroxylase with a substrate. Modulators of HIF hydroxylase are provided for use in the treatment of a condition associated with increased or decreased HIF levels or activity or for the treatment of a condition where it is desirable to modulate HIF levels or activity.Type: ApplicationFiled: September 16, 2013Publication date: October 2, 2014Applicant: Isis Innovation LimitedInventors: Patrick Henry Maxwell, Christopher William Pugh, Peter John Ratcliffe, Christopher Joseph Schofield
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Publication number: 20140295493Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: April 1, 2014Publication date: October 2, 2014Inventors: Hiroaki Udagawa, Christian Isak Jorgensen
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Publication number: 20140295476Abstract: The described invention provides a method of generating a catalytically active oxidase enzyme preparation. The described invention also provides a means of generating reagents for a protease detection sensor which can use colorimetric, fluorescent, or electrochemical detection. Further, the invention describes a system for a protease detecting sensor.Type: ApplicationFiled: March 12, 2014Publication date: October 2, 2014Applicant: Abbott Point of Care Inc.Inventors: Dan Wang, G. Bruce Collier
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Publication number: 20140298502Abstract: The amino acid and nucleic acid sequences of a ?5-desaturase enzyme and a ?8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.Type: ApplicationFiled: June 18, 2014Publication date: October 2, 2014Applicant: Washington State University Research FoundationInventors: John A. Browse, James G. Wallis, Jennifer L. Watts
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Publication number: 20140287426Abstract: Provided are compositions and methods for labeling an endogenous protein, in particular, in a live cell, or for ablating an endogenous or target protein. The compositions relate to a fusion protein having a binding moiety such as an antibody, an antigen binding fragment of an antibody or an antibody mimetic that recognizes the endogenous or target protein.Type: ApplicationFiled: March 13, 2012Publication date: September 25, 2014Applicant: UNIVERSITY OF SOUTHERN CALIFORNIAInventors: Don B. Arnold, Richard W. Roberts, Garrett G. Gross, Rudy Mora, Jason Junge
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Publication number: 20140287477Abstract: Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.Type: ApplicationFiled: October 26, 2012Publication date: September 25, 2014Applicant: DANISCO US INC.Inventors: Luis G. Cascao-Pereira, David A. Estell, Marc Kolkman, Harm Mulder
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Publication number: 20140271599Abstract: Disclosed are compositions and methods for decreasing the viscosity and/or cohesiveness of and/or increasing the liquefaction of excessively or abnormally viscous or cohesive mucus or sputum. The composition contains a protein or peptide containing a thioredoxin monocysteinic active site in a reduced state and optionally further contains a reducing system.Type: ApplicationFiled: March 17, 2014Publication date: September 18, 2014Applicant: OrPro Therapeutics, Inc.Inventor: Peter B. Heifetz
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Publication number: 20140271691Abstract: The present invention encompasses a cancer vaccine therapy targeting Aspartyl-[Asparaginyl]-?-hydroxylase (HAAH). The present invention contemplate bacteriophage expressing HAAH peptide fragments and methods for using said bacteriophage in methods of treating cancer.Type: ApplicationFiled: November 6, 2013Publication date: September 18, 2014Applicant: PANACEA PHARMACEUTICALSInventors: Biswajit Biswas, Carl R. Merril, Hossein A. Ghanbari
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Publication number: 20140273152Abstract: Disclosed herein is a recombinant Bacillus manganese oxidase complex, including a bacterial expression vector for the expression of the complex and a method of synthesizing the complex.Type: ApplicationFiled: March 13, 2014Publication date: September 18, 2014Applicant: Oregon Health & Science UniversityInventors: Bradley M. Tebo, Cristina Butterfield
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Publication number: 20140273039Abstract: The present invention relates to a method of determining Botulinum toxin (BoNT) based on a luminescence assay. The present application further relates to a peptide that is susceptible to proteolytic cleavage by BoNT which is suitable for that method.Type: ApplicationFiled: September 3, 2012Publication date: September 18, 2014Applicant: ALBERT-LUDWIGS-UNIVERSITÄT FREIBURGInventors: Gregory Stevens, Michael Krueger, Andrea Zgaga-Griesz, Gerald Urban, Dalice Silver
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Publication number: 20140273156Abstract: A modified beetle luciferase protein which is an environmentally sensitive reporter protein is provided.Type: ApplicationFiled: February 14, 2014Publication date: September 18, 2014Applicant: PROMEGA CORPORATIONInventors: Frank Fan, Martin Ken Lewis, John W. Shultz, Keith V. Wood, Braeden Butler