Involving Hydrolase Patents (Class 435/18)
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Patent number: 9150842Abstract: The present invention relates to methods of increasing the activity of a GH61 polypeptide having cellulolytic enhancing activity, comprising: adding a divalent copper cation to a composition comprising the GH61 polypeptide having cellulolytic enhancing activity, wherein the presence of the divalent copper cation and the GH61 polypeptide having cellulolytic enhancing activity increases degradation or conversion of a cellulosic material by an enzyme composition compared to the GH61 polypeptide having cellulolytic enhancing activity without the divalent copper cation. The present invention also relates to compositions, methods for degrading or converting a cellulosic material, and methods for producing a fermentation product.Type: GrantFiled: March 9, 2012Date of Patent: October 6, 2015Assignees: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Katja Salomon Johansen, Feng Xu, Paul Walton, Brett McBrayer, Henrik Lund, Chee-Leong Soong
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Patent number: 9145553Abstract: The current disclosure provides methods and kits for isolating nucleic acid from an environmental sample. The current methods and compositions further provide methods for isolating nucleic acids by reducing adsorption of nucleic acids by charged ions and particles within an environmental sample. The methods of the current disclosure provide methods for isolating nucleic acids by releasing adsorbed nucleic acids from charged particles during the nucleic acid isolation process. The current disclosure facilitates the isolation of nucleic acids of sufficient quality and quantity to enable one of ordinary skill in the art to utilize or analyze the isolated nucleic acids for a wide variety of applications including, sequencing or species population analysis.Type: GrantFiled: June 14, 2013Date of Patent: September 29, 2015Assignee: UT-BATTELLE, LLCInventors: Richard Ashley Hurt, Jr., Dwayne A. Elias
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Publication number: 20150147768Abstract: The invention provides a culture plate made from a polymer incorporating a culture component releasable into culture media in the well, methods of culturing a microorganisms in the culture plate, and a methods of making the culture plate.Type: ApplicationFiled: September 20, 2013Publication date: May 28, 2015Applicant: Danisco US IncInventors: Jimmy Chan, Rachel E. Muir, William Throndset
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Publication number: 20150147309Abstract: The present invention relates to an allosteric non-inhibitory chaperone of the lysosomal acid alpha-glucosidase (GAA) for use in the treatment of a pathological condition characterized by a deficiency of the lysosomal acid alpha-glucosidase (GAA), to pharmaceutical composition thereof, to a method for increasing the activity of GAA in a subject and to a method for identifying an allosteric non-inhibitory chaperone for GAA.Type: ApplicationFiled: June 6, 2013Publication date: May 28, 2015Inventors: Giancarlo Parenti, Caterina Porto, Marco Moracci, Maria Carmina Ferrara, Beatrice Cobucci-Ponzano, Generoso Andria
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Publication number: 20150141361Abstract: A pharmaceutical composition including at least one epigenome-modifying compound, for a use thereof in the treatment of genetic muscular diseases linked to a conformational disorder of at least one protein, said disorder causing the cellular degradation of the protein.Type: ApplicationFiled: July 16, 2013Publication date: May 21, 2015Applicant: GENETHONInventor: Isabelle Richard
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Publication number: 20150140576Abstract: The present invention describes glycans, which are specifically expressed by certain cancer cells, tumours and other malignant tissues. The present invention describes methods to detect cancer specific glycans as well as methods for the production of reagents binding to said glycans. The invention is also directed to the use of said glycans and reagents binding to them for the diagnostics of cancer and malignancies. Furthermore, the invention is directed to the use of said glycans and reagents binding to them for the treatment of cancer and malignancies. Moreover, the present invention comprises efficient methods to differentiate between malignant and benign tumors by analyzing glycan structures.Type: ApplicationFiled: October 24, 2014Publication date: May 21, 2015Applicant: GLYKOS FINLAND OYInventors: Tero SATOMAA, Jari NATUNEN, Annamari HEISKANEN, Anne OLONEN, Juhani SAARINEN, Noora SALOVUORI, Jari HELIN
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Publication number: 20150140572Abstract: The present invention relates to a composition having the activity of degrading the cell wall of a Mycobacterium species comprising: (a) a first fusion protein including (i) a domain with a first enzymatic activity, the enzymatic activity being at least one or more of the following: N-acetyl-b-D-muramidase (lysozyme, lytic transglycosylase), N-acetyl-b-D-glucosaminidase, N-acetylmuramoyl-L-alanine amidase, L-alanoyl-D-glutamate (LD) endopeptidase, c-D-glutamyl-meso-diaminopimelic acid (DL) peptidase, D-Ala-m-DAP (DD) endopeptidase, or m-DAP-m-DAP (LD) endopeptidase, (ii) at least one peptide stretch fused to the N- or C-terminus of the domain with the first enzymatic activity; and (iii) a protein transduction domain (PTD) being at the N- or C-terminus of the first fusion protein; and (b) a second fusion protein including (i) a domain with a second enzymatic activity, the enzymatic activity being at least one or more of the following: lipolytic activity, cutinase, mycolarabinogalactanesterase, or alpha/beta hyType: ApplicationFiled: July 1, 2013Publication date: May 21, 2015Inventors: Stefan Miller, Robert Andreas Fischer
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Publication number: 20150140573Abstract: The present invention relates to a method of detecting ?-(1?6)-glucosidase activity in a sample. The method is for example useful for determining the limit dextrinase activity in a sample. The method involves use of an oligosaccharide substrate of the formula X-(glucoside)n-*(glucoside)m-Z—Y, where X is a blocking group, -* is a ?-(1?6)-glucosidic linkage and Y is a detectable label. Upon cleavage of the ?-(1?6)-glucosidic linkage, the detectable label is released and thus the ?-(1?6)-glucosidase activity can be determined. The invention also relates to the oligosaccharide substrate per se.Type: ApplicationFiled: June 7, 2013Publication date: May 21, 2015Inventors: Marie Bøjstrup, Caspar Elo Christensen, Ole Hindsgaul
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Publication number: 20150140560Abstract: The disclosure provides a label-free viscosity-based analyte detection system using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. It is disclosed herein that the bead rotation period is linearly proportional to the viscosity of a solution comprising analytes surrounding the paramagnetic bead. Optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of analyte concentration. The results demonstrate the feasibility of viscosity-based analyte detection using AMBR in microscale aqueous volumes.Type: ApplicationFiled: November 4, 2014Publication date: May 21, 2015Inventors: Raoul Kopelman, Mark A. Burns, Yunzi Li
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Publication number: 20150140587Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.Type: ApplicationFiled: July 14, 2014Publication date: May 21, 2015Inventors: Aldis Darzins, Lance Encell, Tonny Johnson, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
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Patent number: 9034903Abstract: Inhibitors of the soluble epoxide hydrolase (sEH) are provided that incorporate multiple pharmacophores and are useful in the treatment of diseases. In some embodiments, the present invention provides a method for monitoring the activity of a soluble epoxide hydrolase, the method including contacting the soluble epoxide hydrolase with an amount of a compound of the present invention sufficient to produce a detectable change in the fluorescence of the soluble epoxide hydrolase by interacting with one or more tryptophan residues present in the catalytic site of said sEH.Type: GrantFiled: February 1, 2013Date of Patent: May 19, 2015Assignee: The Regents of the University of CaliforniaInventors: Bruce D. Hammock, Kin Sing Lee, Christophe Morisseau
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Publication number: 20150133496Abstract: Described is a method for treating an individual having a neurological disorder with an associated mutation or mutations in a gene encoding a lysosomal enzyme. Specifically, the individual is administered a specific pharmacological chaperone for the lysosomal enzyme which increases trafficking of the protein from the ER to the lysosome in neural cells, with or without concomitantly increasing enzyme activity in neural cells. Restoration of trafficking relieves cell stress and other toxicities associated with accumulation of mutant proteins. Restoration of enzyme activity relieves substrate accumulation and pathologies associated with lipid accumulation. In a specific embodiment, the neurological disorder is Parkinson's disease or parkinsonism which is associated with mutations in glucocerebrosidase.Type: ApplicationFiled: October 3, 2014Publication date: May 14, 2015Inventor: Brandon Alan Wustman
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Patent number: 9029115Abstract: The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, and are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.Type: GrantFiled: August 16, 2012Date of Patent: May 12, 2015Assignees: Novozymes A/S, Novozymes IncInventors: Allan Svendsen, Michael Skjoet, Deborah Yaver, Lars Lehmann Hylling Christensen, Signe Eskildsen Larsen, Nina Lundin, Michael Lamsa, Peter Colin Gregory
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Patent number: 9029401Abstract: The present invention provides compounds for the inhibition of soluble epoxide hydrolase and associated disease conditions.Type: GrantFiled: February 14, 2012Date of Patent: May 12, 2015Assignee: The Regents of the University of CaliforniaInventors: Bruce D. Hammock, Sung Hee Hwang, Aaron T. Wecksler, Christophe Morisseau
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Publication number: 20150126580Abstract: Methods for diagnosing and treating cancer associated with an oncogenic Kras mutation in a subject are provided.Type: ApplicationFiled: December 19, 2012Publication date: May 7, 2015Inventors: Ronald A. DePinho, Lewis Cantley, Alec C. Kimmelman, Haoqiang Ying, Costas A. Lyssiotis
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Publication number: 20150126573Abstract: The present invention relates to methods and compositions for the prevention or treatment of chronic obstructive pulmonary disease.Type: ApplicationFiled: May 7, 2013Publication date: May 7, 2015Inventors: Jorge Bernardo Boczkowski, Serge Adnot, Maylis Dagouassat, Jean-Marie Gagliolo
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Publication number: 20150125888Abstract: A process for enzymatically converting a furanoside substrate in a product of interest, includes contacting the substrate with an enzyme in presence of an alcohol acceptor, wherein the enzyme is preferably Araf51, and wherein the product is preferably an alkyl furanoside. The mutant Araf51 enzyme showing improved transglycosylation activity in comparison with the native wild-type (wt) Araf51 enzyme, and a method for screening the mutants are also described.Type: ApplicationFiled: April 19, 2013Publication date: May 7, 2015Applicants: ECOLE NATIONALE SUPERIEURE DE CHIMIE DE RENNES (ENSCR), CENTRE NATIOANAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)Inventors: Richard Daniellou, Caroline Nugier-Chauvin, Vincent Ferrieres, Alize Pennec, Ilona Chlubnova
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Patent number: 9023631Abstract: Isolated polynucleotides and polypeptides encoded therefrom are provided. These include mutated PON enzymes with increased, modified or substantially the same substrate specificity as compared to respective wild-type PON. Also provided are kits and methods using these enzymes.Type: GrantFiled: October 21, 2013Date of Patent: May 5, 2015Assignee: Yeda Research and Development Co. Ltd.Inventors: Dan S. Tawfik, Amir Aharoni, Leonid Gaydukov, Joel L. Sussman, Israel Silman
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Publication number: 20150118248Abstract: The application describes a method of screening for breast cancer by testing fro the amount of HAGE (Helicase antigen) in a sample of breast tissue. Methods of prognosis of samples of breast cancer tumours are also provided. HAGE+ ER? (estrogen receptor-) cancers are indicated as being amenable to chemotherapy. Methods of treating breast cancers with HAGE-specific CTA antigen or HAGE-specific antibodies are also provided.Type: ApplicationFiled: March 27, 2013Publication date: April 30, 2015Inventors: Adam Linley, Morgan Mathieu, Stephanie Mcardle, Chungui Lu, Robert Rees
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Crystal structure of amino terminal portion of influenza virus polymerase PA subunit and use thereof
Patent number: 9017960Abstract: Present invention discloses the three-dimensional crystal structure of the N-terminus polypeptide of influenza virus polymerase subunit (PA_N of SEQ ID NO:7). PA_N of SEQ ID NO: 1 is residues 1—50 to 150-300 of influenza virus polymerase subunit PA. In the three-dimensional structure, at least 40% of atoms show the same atomic coordinates, compared to that listed in Table 1. Namely, in the three-dimensional structure of influenza virus polymerase subunit PA_N of SEQ ID NO: 7, 40% of atomic coordinates on carbon skeleton of residues of influenza virus polymerase subunit PA_N of SEQ ID NO: 7, show less than or equal to 1.7 ? of average variance, compared to the atomic coordinates listed in Table 1. Present invention also discloses the expression, purification, crystallization methods, and three-dimensional crystal structure of 256 residues in the N-terminus of influenza virus polymerase subunit PA, and applications of the crystal structure of SEQ ID NO: 7 on drug screening and designing.Type: GrantFiled: February 3, 2010Date of Patent: April 28, 2015Assignee: Institute of Biophysics Chinese Academy of SciencesInventors: Yingfang Liu, Zihe Rao -
Patent number: 9017961Abstract: Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.Type: GrantFiled: March 5, 2012Date of Patent: April 28, 2015Assignee: E.I. du Pont de Nemours and CompanyInventors: Kristin Ruebling-Jass, Jean-Francois Tomb, Tina K Van Dyk, Zheng You
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Publication number: 20150111228Abstract: The present disclosure provides an optical biosensor including a complex including a porous support and an enzyme supported inside the pores of the porous support and a method for preparing the same. Since a dye does not flow but is concentrated in one place, the optical biosensor of the present disclosure has remarkably improved sensitivity. In addition, it is possible to carry out quantitative determination and qualitative analysis since color intensity increases with time.Type: ApplicationFiled: May 23, 2013Publication date: April 23, 2015Applicants: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Jungbae Kim, Min-Gon Kim, Hyou-Arm Joung, Yongho Wee
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Publication number: 20150111786Abstract: The invention provides methods of treating a meiotic kinase-associated disease, preferably the meiotic kinase HSET, by administering an inhibitor of the meiotic kinase. Preferably, the disease is associated with the presence of supernumerary centrosomes, such as cancer. Methods of inhibiting the growth of a tumor cell by contacting the cell with an inhibitor of a meiotic kinase, preferably HSET, are also provided. Screening methods for identifying inhibitors of the meiotic Kinase HSET are also provided. Methods of selecting subjects for treatment with an inhibitor of a meiotic kinase, such as HSET, are also provides.Type: ApplicationFiled: January 7, 2015Publication date: April 23, 2015Applicant: DANA-FARBER CANCER INSTITUTE, INC.Inventor: David Pellman
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Publication number: 20150112045Abstract: Disclosed is a transformed microalga including a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an amino acid sequence including (i) an heterologous signal peptide; and (ii) a therapeutic antibody, a functional fragment or a derivative thereof, the transformed microalga expressing the therapeutic antibody, functional fragment or derivative thereof secreted in the extracellular media and the microalga being selected among green algae except Volvocales, and among red algae, chromalveolates, and euglenids. Preferably, therapeutic antibody, functional fragment or derivative thereof has an increased antibody-dependant cell-mediated cytotoxicity (ADCC) and a low fucose content.Type: ApplicationFiled: May 2, 2013Publication date: April 23, 2015Applicant: ALGENICSInventors: Aude Carlier, Remy Michel, Alexandre Lejeune, Jean-Paul Cadoret
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Publication number: 20150111237Abstract: The invention relates to methods for enzymatic detection of biotinidase activity. In certain aspects, the invention provides methods for bench-based enzymatic detection of biotinidase activity. In other aspects, the invention provides methods for bench-based enzymatic detection in droplets in oil. The droplet-based methods may be performed in an automated manner using digital microfluidics technology on a droplet actuator device. The enzymatic assays for biotinidase activity may be used for newborn testing for biotinidase deficiency, and may be combined with other droplet-based enzymatic assays in a panel of tests for newborn testing.Type: ApplicationFiled: May 7, 2013Publication date: April 23, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Carrie A. Graham, Allen E. Eckhardt
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Patent number: 9012167Abstract: The present invention concerns an in vitro method for detecting an enzyme or a microorganism in a biological sample, comprising the steps of: a1) concentrating the microorganisms present in the biological sample, optionally after a a0) culture step of the microorganisms; b1) placing in suspension the microorganisms concentrated at step a1) in a solution containing at least one chromogenic or fluorogenic substrate capable of releasing a chromophore or fluorophore after hydrolysis by the enzyme to be detected; c1) detecting potential release of the chromophore or fluorophore obtained at step b1); the release of the chromophore or fluorophore detected at step c1) indicating the presence of the enzyme to be detected.Type: GrantFiled: March 1, 2011Date of Patent: April 21, 2015Assignee: Bio-Rad InnovationsInventors: Caroline Dallenne, Christine Favier
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Patent number: 9011821Abstract: In some aspects, the invention provides compositions and methods for inhibiting viral infection. In some aspects, the invention provides compositions and methods useful for identifying antiviral compounds.Type: GrantFiled: June 17, 2011Date of Patent: April 21, 2015Assignee: Whitehead Institute for Biomedical ResearchInventors: Thijn R. Brummelkamp, Jan E. Carette
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Patent number: 9012229Abstract: A capillary electrophoresis method for quantitatively analyzing characteristic oligosaccharide present in enoxaparin sodium is provided in this invention. The method may be used for quantitatively determining the contents of disaccharides, trisaccharides, tetrasaccharides and in particular oligosaccharides having a 1,6-anhydro ring, which are unique compounds for enoxaparin sodium, within an exhaustively digested enoxaparin sodium sample with a mixture of heparinase I, II, and III, so as to quantitatively determine the molar percentage of oligosaccharides having 1,6-anhydro ring in enoxaparin sodium. The method may be used for the pharmaceutical quality control of enoxaparin sodium during the manufacturing process.Type: GrantFiled: January 20, 2012Date of Patent: April 21, 2015Assignees: Hangzhou Jiuyuan Gene Engineering Co., Ltd., Shanghai Institute of Organic Chemistry, Chinese Academy of SciencesInventors: Jingwu Kang, Xueqiang Zhan
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Publication number: 20150099669Abstract: The presently disclosed subject matter provides methods using mass spectrometry for direct profiling of N-linked glycans from a biological sample. In addition, the embodiments of the present invention also disclose novel methods, known as targeted analyte detection (TAD), for improving the detection limit of MALDI-MS. These methods take advantage of the carrier effect of the added standard analytes, which occurs due to the generic sigmoidal shape of the calibration curve. The functionality of TAD depends on the relative enhancement of sensitivity over the increase of the standard deviation at the analysis of target analytes with spiking in exogenous concentration. At certain ranges of exogenous concentration, the increment in the sensitivity overcomes the standard deviation, resulting in an improved LOD. Theoretically, exogenous concentrations approximately at 1 LODorig would generate the optimum LOD improvement.Type: ApplicationFiled: May 23, 2013Publication date: April 9, 2015Inventors: Hui Zhang, Xingde Li, Shadi Toghi Eshghi
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Publication number: 20150099664Abstract: A method for characterizing the risk a subject will develop an autoimmune and/or alloimmune disease following tissue transplant includes obtaining a biological sample from the subject, wherein the subject has received the tissue transplant determining in the biological sample a level of at least one protein selected from Tables 1-4, comparing the measured level of the at least one protein to a control value, and characterizing a subject as at greater risk of developing an autoimmune disease and/or alloimmune disease if the level of at least one protein determined is increased or decreased compared to the control value.Type: ApplicationFiled: December 15, 2014Publication date: April 9, 2015Inventors: Mark Chance, Kenneth Cooke, Daniela Schlatzer
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Publication number: 20150099803Abstract: The present invention is directed to methods for treatment of malignancies in companion animals employing dianhydrogalactitol, diacetyldianhydrogalactitol, and dibromodulcitol, as well as analogs and derivatives thereof, in addition to a method to improve the efficacy and/or reduce the side effects of the administration of a therapeutic agent selected from the group consisting of dianhydrogalactitol, a derivative of dianhydrogalactitol, diacetyldianhydrogalactitol, a derivative of diacetyldianhydrogalactitol, dibromodulcitol, and a derivative of dibromodulcitol to a veterinary subject, the method comprising the steps of: (1 (identifying at least one factor or parameter associated with the efficacy and/or occurrence of side effects of the administration of the therapeutic agent to the veterinary subject; and (2) modifying the factor or parameter to improve the efficacy and/or reduce the side effects of the administration of the therapeutic agent to the veterinary subject.Type: ApplicationFiled: May 3, 2013Publication date: April 9, 2015Inventors: Jeffrey A. Bacha, Dennis M. Brown, William A. Garner
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Publication number: 20150099712Abstract: A method for treating cancer by using an agent which is capable of inhibiting the functionality of the MCM complex, a heterohexameric ring formed from six subunits, in the process of DNA replication and a method of screening for such agents by detecting the locations and functions of the MCM subunits, such as hMcm2 and hMcm6, in cells treated with candidate compounds.Type: ApplicationFiled: May 9, 2013Publication date: April 9, 2015Inventors: Chun Liang, Zhihong Jiang, Ziyi Wang, Zhiling Yu, Jingrong Wang, Liping Bai
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Patent number: 8980605Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: March 11, 2013Date of Patent: March 17, 2015Assignee: Codexis, Inc.Inventors: Charlene Ching, John M. Gruber, Gjalt W. Huisman, Emily Mundorff, Lisa M. Newman
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Publication number: 20150071894Abstract: Method of in vitro measurement of the presence of factors that are able to neutralize asparaginase activity in a sample of blood, plasma, serum or derived medium that may contain asparaginase neutralizing factors, obtained from a patient, comprising mixing of said sample with asparaginase, incubation of said mixture, then measurement of the residual asparaginase activity in the mixture and determination or quantification of the presence of said neutralizing factors. Method for predicting the efficacy of a treatment with asparaginase.Type: ApplicationFiled: September 18, 2014Publication date: March 12, 2015Inventor: Yann GODFRIN
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Patent number: 8975043Abstract: Novel enzymes, processes and antigenic structures useful in producing vaccines and compounds useful in combating gram-negative bacteria are described. Enzymes were isolated from the slime mold Dictyostelium discoideum and used to specifically degrade lipopolysaccharide (LPS). Enzymatic degradation permits residues of the LPS molecule, including immunogenic epitopes of the core oligosaccharide portion of the LPS, to remain unmodified during this enzymatic removal of fatty acids from the lipid A region of the LPS molecule.Type: GrantFiled: November 23, 2006Date of Patent: March 10, 2015Assignee: National Research Council of CanadaInventors: Andrew Cox, Dhamodharan Neelamegan, Ian Schoenhofen, Frank St. Michael, James Richards
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Patent number: 8975055Abstract: The present invention relates to a newly identified lipase belonging to the Ustilaginaceae family of Basidomycetes and variants thereof. The invention also relates to polynucleotides encoding the lipase and the variants thereof. The invention further relates to procedures for producing the lipase, and variants thereof, polypeptides and polynucleotides. The invention further relates to lipase variants with an increased trans-selectivity. The invention further relates to lipase variants with a preference for long chain fatty acid esters or carboxylic acid moieties with a chain length greater than C12. The invention further relates to a method of reducing, or eliminating, trans-fatty acids from liquid oil stable enough for frying systems.Type: GrantFiled: March 11, 2013Date of Patent: March 10, 2015Assignee: Frito-Lay Trading Company GmbHInventors: Uwe Bornscheuer, Henrike Brundiek, Andrew Evitt, Stefan SaB, Friedericke Bonisch, Robert Kourist
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Publication number: 20150064715Abstract: The present invention provides methods of detecting mitochondrial dysfunction or acute kidney injury (AKI) by measuring the urinary protein levels of the ATP synthase (ATPS) beta subunit or cleavage products thereof in a subject.Type: ApplicationFiled: August 28, 2014Publication date: March 5, 2015Applicant: MUSC FOUNDATION FOR RESEARCH DEVELOPMENTInventors: Rick SCHNELLMANN, John ARTHUR, Craig BEESON
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Publication number: 20150064714Abstract: Labelled polypeptide constituted by: (i) a peptide fragment of 38 to 50 amino acids including a peptide of sequence SEQ ID NO: 1, and (ii) a label selected from the group constituted by radioactive groups, non-protein fluorophors, protein fluorophors, magnetic particles or a paramagnetic spin label, in order that when the label is a fluorophor protein, the labelled polypeptide further contains a peptide spacer of 3 to 15 amino acids, located between the peptide fragment and the label and use thereof as a diagnostic tool.Type: ApplicationFiled: December 3, 2013Publication date: March 5, 2015Applicant: LIFESEARCHInventor: Tiona ANDRIANAIVOMANANJAONA
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Publication number: 20150065478Abstract: This invention comprises the novel compounds of formula (I) wherein n, m, t, R1, R2, L, Q, X, Y, Z and have defined meanings, having histone deacetylase inhibiting enzymatic activity; their preparation, compositions containing them and their use as a medicine.Type: ApplicationFiled: November 11, 2014Publication date: March 5, 2015Inventors: Patrick Rene Angibaud, Kristof Van Emelen, Virginie Sophie Poncelet, Bruno Roux
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Patent number: 8969061Abstract: Compositions, methods and a kit are described relating to a novel family of enzymes which preferentially bind to a hydroxymethylated cytosine or a glucosylated hydroxymethylated cytosine and cleave double-stranded DNA at a defined distance 3? of the recognition site to produce a cleavage fragment with a 1-3 base overhang.Type: GrantFiled: January 20, 2011Date of Patent: March 3, 2015Assignee: New England Biolabs, Inc.Inventors: Zhenyu Zhu, Yu Zheng, Shengxi Guan, Hua Wang, Aine Quimby, Penghua Zhang, Lynne Apone
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Publication number: 20150057236Abstract: Sirt5, a mitochondrial Sirtuin, has been identified herein as an efficient demalonylase and desuccinylase. Disclosed herein are assays to identify Sirt5 modulators based on this robust enzymatic activity. Sirt5-specific modulators can be used study the biological function of Sirt5 and to target Sirt5 activities in treating human diseases.Type: ApplicationFiled: July 7, 2011Publication date: February 26, 2015Applicant: CORNELL UNIVERSITYInventor: Hening Lin
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Patent number: 8962302Abstract: A biological tissue processing substrate for fixing proteins in a biological tissue or degradation products of the proteins, the substrate comprising: a porous body that forms a contact surface with the biological tissue, the porous body holding in pores an enzyme for obtaining the proteins or the degradation products of the proteins from the biological tissue, wherein the proteins or the degradation products obtained by the action of the enzyme are brought into contact with a member consisting of a metal.Type: GrantFiled: November 3, 2006Date of Patent: February 24, 2015Assignee: Canon Kabushiki KaishaInventors: Kazuhiro Ban, Miki Ogawa, Norihiko Utsunomiya, Hiroyuki Hashimoto, Manabu Komatsu, Yohei Murayama
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Patent number: 8962295Abstract: The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.Type: GrantFiled: June 1, 2011Date of Patent: February 24, 2015Assignee: California Institute of TechnologyInventors: Frances H. Arnold, Pete Heinzelman
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Patent number: 8961962Abstract: The present invention relates to methods of promoting the survival of cells by treating the cells with acid ceramidase. A kit for promoting ex vivo cell survival is also disclosed, as is a method of predicting in vitro fertilization outcome of a female subject.Type: GrantFiled: January 7, 2008Date of Patent: February 24, 2015Assignee: Mount Sinai School of MedicineInventors: Edward H. Schuchman, Efrat Eliyahu, Nataly Shtraizent, Xingxuan He
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Publication number: 20150051288Abstract: Disclosed herein are methods and compositions for treating autism. Disclosed herein are methods and compositions for treating an autism spectrum disorder.Type: ApplicationFiled: October 10, 2014Publication date: February 19, 2015Inventors: Paul Talalay, Andrew W. Zimmerman, Kirby D. Smith
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Publication number: 20150050679Abstract: The present invention provides a method and test for the determination of the presence or absence of an antibiotic in a sample such as milk.Type: ApplicationFiled: October 18, 2012Publication date: February 19, 2015Inventors: Dhiredj Chandre Jagesar, Tim De Graaf, Truke Widel Smoor
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Publication number: 20150045389Abstract: The present invention is a screening assay for identifying inhibitors of Pseudomonas aeruginosa CFTR Inhibitory Factor as well as compounds identified by the screening assay for use in compositions and methods for ameliorating or treating a respiratory disease such as cystic fibrosis or secondary infection thereof.Type: ApplicationFiled: April 9, 2013Publication date: February 12, 2015Inventors: Dean R. Madden, Christopher D. Bahl, Bruce D. Hammock, Christophe Morisseau
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Publication number: 20150045284Abstract: The present invention relates to crystals of a type IB P-type ATPase having the space group P1 and methods for purification and growing said crystals. The invention also presents methods for identifying an inhibitor of a type IB P-type ATPase for example by determining binding interactions between an inhibitor and a set of binding interaction sites in said type IB P-type ATPase.Type: ApplicationFiled: January 20, 2012Publication date: February 12, 2015Applicant: Aarhus UniversitetInventors: Poul Nissen, Pontus Gourdon, Xiang Liu, Bjorn Panyella Pedersen, Daniel Mattle
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Patent number: 8951770Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: December 9, 2010Date of Patent: February 10, 2015Assignee: Novozymes A/SInventors: Lars Beier, Miguel Duarte Toscano, Esben Peter Friis, Merete Moeller Engelsen, Henrik Lundkvist, Sune Sauer Lobedanz
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Patent number: 8951752Abstract: The invention provides isolated nucleic acid encoding one or more gene products that allow for degradation of caffeine and related structures, and for preparation of intermediates in that catabolic pathway. Further provided are methods of using one or more of those gene products.Type: GrantFiled: October 18, 2012Date of Patent: February 10, 2015Assignee: University of Iowa Research FoundationInventors: Venkiteswaran Subramanian, Michael Tai-Man Louie, Ryan Summers