Acting On A Linear Amide Linkage In Linear Amide Patents (Class 435/228)
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Patent number: 7592168Abstract: A CPC acylase mutant of the present invention has an improved reactivity and specific activity to CPC, which can be efficiently employed for directly preparing 7-ACA from CPC by a one-step enzymatic method.Type: GrantFiled: August 10, 2004Date of Patent: September 22, 2009Assignee: Sandoz AGInventors: Yong Chul Shin, John Y J Jeon, Kyung Hwa Jung, Mi Ran Park, Youngso Kim
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Patent number: 7582454Abstract: The present invention relates to a 5-substituted hydantoin racemase, which efficiently catalyzes racemization reactions at a high optimum temperature for racemization reactions, DNA coding for the racemase, and processes for producing optically active amino acids.Type: GrantFiled: July 12, 2006Date of Patent: September 1, 2009Assignee: Ajinomoto Co. Inc.Inventors: Shunichi Suzuki, Norimasa Onishi, Kenzo Yokozeki
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Patent number: 7575883Abstract: Modulation of cytochrome c acetylation, e.g., with a SIR polypeptide, enables interventions that modulate lifespan regulation and cell proliferation, e.g., by modulating apoptosis and/or mitochondrial function such as respiration.Type: GrantFiled: December 15, 2003Date of Patent: August 18, 2009Assignee: Elixir Pharmaceuticals, Inc.Inventors: L. Julie Huber, Jonathan M. Solomon
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Patent number: 7553652Abstract: The present invention relates to a novel process for the preparation of (S)— or (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid and to novel microorganisms capable of utilizing the propionamide of the formula in the form of the racemate or of its optically active isomers as the sole nitrogen source.Type: GrantFiled: August 10, 2005Date of Patent: June 30, 2009Assignee: Lonza AGInventors: Walter Brieden, Andrew Naughton, Karen Robins, Nicholas Shaw, Andreas Tinschert, Thomas Zimmermann
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Publication number: 20090061500Abstract: A method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes, or a new bacterium Chryseobacterium sp. No. 9670 belonging to the genus Chryseobacterium, and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of the enzyme, and subsequently collecting the enzyme from the culture mixture and a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein, as well as a gene which encodes the enzyme, a recombinant vector which contains the gene, a transformant transformed with the vector and a method in which the transformant is cultured in a medium to effect production of the protein-deamidating enzyme and then the protein-deamidating enzyme is collected from the culture mixture.Type: ApplicationFiled: July 23, 2008Publication date: March 5, 2009Applicant: Amano Enzyme Inc.Inventor: Shotaro Yamaguchi
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Patent number: 7491521Abstract: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.Type: GrantFiled: August 20, 2007Date of Patent: February 17, 2009Assignee: Evonik Degussa GmbHInventors: Steffen Osswald, Stefan Verseck, Uta Deiting, Christoph Weckbecker, Klaus Huthmacher, Michael Binder, Maria-Regina Kula, Konrad Odendahl
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Publication number: 20090042230Abstract: The present invention relates to flea allantoinase proteins; to flea allantoinase nucleic acid molecules, including those that encode such flea allantoinase proteins; to antibodies raised against such proteins; and to compounds that inhibit the activity of such proteins. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. The present invention also includes therapeutic compositions comprising such inhibitory compounds, particularly those that specifically inhibit flea allantoinase activity, as well as the use of such therapeutic compositions to treat animals.Type: ApplicationFiled: March 19, 2008Publication date: February 12, 2009Inventors: PATRICK J. GAINES, NANCY WISNEWSKI
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Patent number: 7473545Abstract: Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.Type: GrantFiled: February 28, 2007Date of Patent: January 6, 2009Assignee: E.I. Du Pont De Nemours +CompanyInventors: Robert DiCosimo, Anna Panova, Daniel P. O'Keefe, Jeffery Thompson, Mark S. Payne
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Patent number: 7445917Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: December 21, 2005Date of Patent: November 4, 2008Assignee: E.I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Patent number: 7432086Abstract: A recombinant microorganism is produced by introducing a DNA encoding an enzyme which hydrolyzes an amido bond of L-amino acid amide, especially L-2-alkylcysteine amide, and L-amino acid is produced by using cells or cell processed product of the obtained microorganism.Type: GrantFiled: May 23, 2005Date of Patent: October 7, 2008Assignee: Mitsubishi Gas Chemical Company, Inc.Inventors: Yasuhisa Asano, Atsushi Inoue
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Publication number: 20080241895Abstract: The present invention provides genes that encode the N-acetyl-(R,S)-?-amino acid acylases. The N-acetyl-(R,S)-?-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-?-amino acid acylase produced by Variovorax sp.Type: ApplicationFiled: July 24, 2007Publication date: October 2, 2008Inventors: Shunichi Suzuki, Yuki Imabayashi, Kunihiko Watanabe, Hisashi Kawasaki, Tsuyoshi Nakamatsu
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Patent number: 7405065Abstract: The invention relates to a novel process for the preparation of (1R,4S)- or (1S,4R)-1-amino-4-(hydroxy-methyl)-2-cyclopentene of the formulae and/or of (1S,4R)- or (1R,4S)-amino alcohol derivatives of the general formulae and to novel microorganisms which are able to utilize a cyclopentene derivative of the general formula as sole nitrogen source, as sole carbon source or as sole carbon and nitrogen source.Type: GrantFiled: July 21, 2004Date of Patent: July 29, 2008Assignee: Lonza AGInventors: Christine Bernegger-Egli, Olwen M. Birch, Pierre Bossard, Walter Brieden, Frank Brux, Knut Burgdorf, Laurent Duc, Kay-Sarah Etter, Yves Guggisberg, Martin Sauter, Eva Maria Urban
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Patent number: 7405064Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.Type: GrantFiled: November 16, 2006Date of Patent: July 29, 2008Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark S. Payne, Robert DiCosimo, John E. Gavagan, Robert D. Fallon
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Patent number: 7358071Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidovorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.Type: GrantFiled: November 20, 2006Date of Patent: April 15, 2008Assignee: E.I. du Pont de Nemours and CompanyInventors: Mark S. Payne, Robert DiCosimo, Daniel P. O'Keefe
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Patent number: 7348173Abstract: The soporific activity of cis-9,10-octadecenoamide and other soporific fatty acid primary amides is neutralized by hydrolysis in the presence of fatty-acid amide hydrolase (FAAH). Hydrolysis of cis-9,10-octadecenoamide by FAAH leads to the formation of oleic acid, a compound without soporific activity. FAAH has be isolated and the gene encoding FAAH has been cloned, sequenced, and used to express recombinant FAAH. Inhibitors of FAAH are disclosed to block the hydrolase activity.Type: GrantFiled: February 26, 2004Date of Patent: March 25, 2008Assignee: The Scripps Research InstituteInventors: Norton B. Gilula, Benjamin F. Cravatt, Richrd A. Lerner
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Patent number: 7294493Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.Type: GrantFiled: November 16, 2006Date of Patent: November 13, 2007Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark S. Payne, Robert DiCosimo, John E. Gavagan, Robert D. Fallon
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Patent number: 7288402Abstract: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.Type: GrantFiled: March 18, 2005Date of Patent: October 30, 2007Assignee: Degussa AGInventors: Steffen Osswald, Stefan Verseck, Uta Deiting, Christoph Weckbecker, Klaus Huthmacher, Michael Binder, Maria-Regina Kula, Konrad Odendahl
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Patent number: 7285406Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitrites to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.Type: GrantFiled: November 16, 2006Date of Patent: October 23, 2007Assignee: E.I. du Pont de Nemours and CompanyInventors: Mark S. Payne, Robert DiCosimo, John E. Gavagan, Robert D. Fallon
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Patent number: 7198927Abstract: Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.Type: GrantFiled: December 21, 2005Date of Patent: April 3, 2007Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Mark S. Payne, Anna Panova, Jeffrey Thompson, Daniel P. O'Keefe
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Patent number: 7186540Abstract: A completely novel glutaminase is provided: (a) a protein consisting of an amino acid sequence represented by SEQ ID NO: 2, or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one or more amino acids, and possessing glutaminase activity. This protein is a novel glutaminase possessing excellent thermostability and the like.Type: GrantFiled: December 24, 2002Date of Patent: March 6, 2007Assignees: National Institute of Advanced Indusrtial Science and Technology, National Institute of Technology and Evaluation, National Research Institute of BrewingInventors: Masayuki Machida, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Motoaki Sano, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara, Kotaro Ito, Kenichiro Matsushima, Yasuji Koyama
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Patent number: 7183094Abstract: The present invention provides a family of bacterial acyl glucosaminylinositol amidases with amidase activity against S-conjugate amides, particularly mycothiol-derived S-conjugate amides. The invention amidases are characterized by a highly conserved 20 amino acid N-terminal region and four highly conserved histidine-containing regions and by having amidase activity, particularly amide hydrolase activity. The invention further provides methods for using the invention amidases in drug screening assays to determine compounds with antibiotic activity or compounds that inhibit activity or production of endogenous acyl glucosaminyl inositol amidase in bacteria. The invention further provides methods for detoxifying a toxic substance by contacting the toxic substance with an invention amidase, for example, by expression of the amidase under environmental conditions in a bacterium.Type: GrantFiled: April 30, 2003Date of Patent: February 27, 2007Assignees: The Regents of the University of California, The University of British ColumbiaInventors: Gerald L. Newton, Yossef Av-Gay, Robert C. Fahey
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Patent number: 7157252Abstract: Glutaminase is purified from an Aspergillus oryzae, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from Aspergillus oryzae genomic library and cDNA library, and Aspergillus nidulans genomic library by hybridization using the partial sequence as a probe.Type: GrantFiled: May 24, 2004Date of Patent: January 2, 2007Assignee: Ajinomoto Co., Inc.Inventors: Kyoko Koibuchi, Hiroaki Nagasaki, Ari Yuasa, Jiro Kataoka, Katsuhiko Kitamoto
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Patent number: 7153663Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.Type: GrantFiled: October 29, 2004Date of Patent: December 26, 2006Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark S. Payne, Robert DiCosimo, John E. Gavagan, Robert D. Fallon
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Patent number: 7129077Abstract: Reagents that regulate human aminopeptidase N and reagents which bind to human aminopeptidase N gene products can play a role in preventing, ameliorating, or correcting dysfunctions or diseases including, but not limited to, cancer, a CNS disorder or COPD.Type: GrantFiled: June 28, 2002Date of Patent: October 31, 2006Assignee: Bayer HealthCare AGInventor: Alex Smolyar
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Patent number: 7112425Abstract: The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides and diketopiperazine derivatives 3- and 6- substituted by ?,?-unsaturated amino acid side chains.Type: GrantFiled: June 18, 2003Date of Patent: September 26, 2006Assignees: Commissariat A L'Energie Atomique, Centre National de la Recherche ScientifiqueInventors: Muriel Gondry, Roger Genet, Sylvie Lautru, Jean-Luc Pernodet
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Patent number: 7101699Abstract: A polypeptide possessing a sphingolipid ceramide deacylase activity; a nucleic acid encoding the polypeptide; a recombinant DNA comprising the nucleic acid; a carrier for introducing into a cell, carrying the nucleic acid or the recombinant DNA; a transformant harboring the nucleic acid or the recombinant DNA; an oligonucleotide probe or primer for the nucleic acid; a method for detecting the nucleic acid using the probe or the primer and a kit usable therefor; an antibody or a fragment thereof against the polypeptide; and a method for detecting the polypeptide using the antibody or a fragment thereof and a kit usable therefor. According to the present invention, there can be applied to engineering of sphingolipids, and to treatments for diseases such as diseases of nervous system (for instance, neurodegenerative diseases and the like), leukemia and wounds.Type: GrantFiled: September 26, 2001Date of Patent: September 5, 2006Assignee: Takara Bio Inc.Inventors: Makoto Ito, Masako Furusato, Noriyuki Sueyoshi
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Patent number: 7074606Abstract: The present invention relates to a novel process for the preparation of (S)- or (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid and to novel microorganisms capable of utilizing the propionamide of the formula in the form of the racemate or of its optically active isomers as the sole nitrogen source.Type: GrantFiled: February 28, 2002Date of Patent: July 11, 2006Assignee: Lonza AGInventors: Walter Brieden, Andrew Naughton, Karen Robins, Nicholas Shaw, Andreas Tinschert, Thomas Zimmermann
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Patent number: 7070963Abstract: The present invention relates to an amidase enzyme, nucleic acids encoding the amidase, as well as methods of employing the nucleic acids and/or amidase to produce, for example, enantiomericallyenriched compounds such as D-amino acids.Type: GrantFiled: December 4, 2002Date of Patent: July 4, 2006Assignee: Degussa AGInventors: Stefan Verseck, Karlheinz Drauz, Andreas Bommarius, Maria-Regina Kula, Lutz Krieg, Heike Slusarczyk, Marion Ansorge-Schumacher
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Patent number: 6953677Abstract: A novel rec-L-N-carbamoylase from Arthrobacter aurescens and its method of use for producing L-amino acids. The recombinantly produced L-carbamoylase is unexpectedly stable, so that an industrial method of producing L-amino acids can be established with it, in contrast to previously known L-carbamoylases.Type: GrantFiled: January 15, 2002Date of Patent: October 11, 2005Assignees: Degussa AG, Universitat StuttgartInventors: Josef Altenbuchner, Ralf Mattes, Markus Pietzsch, Christoph Syldatk, Anja Wiese, Burkard Wilms
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Patent number: 6942990Abstract: The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-?-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.Type: GrantFiled: January 17, 2003Date of Patent: September 13, 2005Assignee: Bristol Myers Squibb CompanyInventors: Ross Binder, Joanne L. Brown, Thomas J. Franceschini, William V. Burnett, Michael Politino, Suo Win Liu, Sean M. Tonzi
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Patent number: 6902915Abstract: The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective, the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).Type: GrantFiled: February 7, 2003Date of Patent: June 7, 2005Assignee: Daicel Chemical Industries, Ltd.Inventors: Shinji Tokuyama, Akinobu Matsuyama
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Patent number: 6897058Abstract: Method for the preparation of an enantiomerically enriched acylated amine wherein a phenylacetic acid derivative is brought into contact with a mixture of enantiomers of an amine H2NCR1R2R3, in which R1, R2, R3 are not equal to each other and each independently stand for H, CN, a whether or not substituted (cyclo)alkyl, ary-, alkylaryl or arylalkyl group, whether or not cyclic heteroalkyl or heteroaryl group with one or more N, O or S atoms or in which R1 and R2 (and R3) together with the C-atom to which they have been bound form a (bi)cyclic group that optionally contains one or more N, O of S atoms, in the presence of a Pen-G acylase derived from Alcaligenes faecalis. And method for the preparation of enantiomerically enriched amine of formula H2NCR1R2R3, in which R1, R2, R3 are as defined above, wherein an acylated amine is brought into contact with a Pen-G acylase originating from Alcaligenes faecalis.Type: GrantFiled: August 30, 2001Date of Patent: May 24, 2005Assignee: DSM IP Assets B.V.Inventors: Vytautas-Juozapas Kajetono {hacek over (S)}vedas, Dorel Teodor Guranda, Roger Arthur Sheldon, Frederik Rantwijk Van, Lukas Michaël Langen Van
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Patent number: 6864080Abstract: Staphylococcus aureus peptide deformylase has been crystallized, and the three-dimensional x-ray crystal structure has been solved to 1.9 ? resolution. The x-ray crystal structure is useful for solving the structure of other molecules or molecular complexes, and designing modifiers of peptide deformylase activity.Type: GrantFiled: June 29, 2001Date of Patent: March 8, 2005Assignee: Pharmacia & Upjohn CompanyInventors: Eric T. Baldwin, Melissa S. Harris
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Patent number: 6849432Abstract: The invention is a process for continuously producing an amide compound by reacting a microorganism fungus body containing nitrile hydratase or a processed product of the microorganism fungus body with a nitrile compound in an aqueous medium, characterized in that after contacting said fungus body or said processed product of said fungus body with said nitrile compound in said aqueous medium, a reaction solution thus obtained is further subjected to reaction under conditions having a plug flow region, and according to the invention, an amide compound aqueous solution of a high concentration a high purity can be easily obtained in an extremely high conversion rate of a nitrile compound without a condensation process.Type: GrantFiled: March 23, 2001Date of Patent: February 1, 2005Assignee: Mitsui Chemicals, Inc.Inventors: Takeya Abe, Kiyoshi Ito, Kenju Sasaki, Seiichi Watanabe, Toshihisa Tachibana, Tamotsu Asano
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Publication number: 20040265958Abstract: The soporific activity of cis-9,10-octadecenoamide and other soporific fatty acid primary amides is neutralized by hydrolysis in the presence of fatty-acid amide hydrolase (FAAH). Hydrolysis of cis-9,10-octadecenoamide by FAAH leads to the formation of oleic acid, a compound without soporific activity. FAAH has be isolated and the gene encoding FAAH has been cloned, sequenced, and used to express recombinant FAAH. Inhibitors of FAAH are disclosed to block the hydrolase activity.Type: ApplicationFiled: February 26, 2004Publication date: December 30, 2004Applicant: The Scripps Research InstituteInventors: Norton B. Gilula, Benjamin F. Cravatt, Richrd A. Lerner
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Publication number: 20040265984Abstract: Methods of growing crystals of free, antibiotic complexed, or ribosomal substrate complexed large ribosomal subunits, coordinates defining the three-dimensional atomic structure thereof and methods of utilizing such coordinates for rational design or identification of antibiotics or large ribosomal subunits having desired characteristics are disclosed.Type: ApplicationFiled: August 25, 2004Publication date: December 30, 2004Inventors: Ada Yonath, Anat Bashan, Raz Zarivach, Francois Francheschi, Frank Schluenzen, Joerg Harms, Renate Albrecht, Rita Berisio
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Publication number: 20040265985Abstract: The invention relates to a novel process for the preparation of (1R,4S)- or (1S,4R)-1-amino-4-(hydroxymethyl)-2-cyclopentene of the formulae 1Type: ApplicationFiled: July 21, 2004Publication date: December 30, 2004Inventors: Christine Bernegger-Egli, Olwen M. Birch, Pierre Bossard, Walter Brieden, Frank Brux, Knut Burgdorf, Laurent Duc, Kay-Sarah Etter, Yves Guggisberg, Martin Sauter, Eva Maria Urban
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Patent number: 6830919Abstract: A neutral/alkaline ceramidase derived from a mammal; an antibody specifically binding thereto; a probe and primer which are capable of specifically hybridizing thereto; a method for producing the ceramidase by a genetic engineering means; a method for detecting the ceramidase or the gene; and a method of controlling an amount of a ceramide in a cell and/or in a tissue. The present invention is useful as a reagent for lipid engineering for analyzing a structure, functions, and the like of a ceramide, and in its applications to diseases associated with the ceramide metabolism.Type: GrantFiled: September 26, 2001Date of Patent: December 14, 2004Assignee: Takara Bio Inc.Inventor: Makoto Ito
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Patent number: 6830905Abstract: Glutaminase is purified from an Aspergillus oryzae, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from Aspergillus oryzae genomic library and cDNA library, and Aspergillus nidulans genomic library hybridization using the partial sequence as a probe.Type: GrantFiled: October 2, 2002Date of Patent: December 14, 2004Assignee: Ajinomoto Co., Inc.Inventors: Kyoko Koibuchi, Hiroaki Nagasaki, Ari Yuasa, Jiro Kataoka, Katsuhiko Kitamoto
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Patent number: 6821761Abstract: The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.Type: GrantFiled: May 20, 2002Date of Patent: November 23, 2004Assignee: Takara Bio, Inc.Inventors: Makoto Ito, Toyohisa Kurita, Katsuhiro Kita, Noriyuki Sueyoshi, Susumu Mitsutake, Masanori Fujita, Nozomu Okino, Hiroyuki Izu, Ikunoshin Kato
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Publication number: 20040229322Abstract: Glutaminase is purified from an Aspergillus oryzae, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from Aspergillus oryzae genomic library and cDNA library, and Aspergillus nidulans genomic library by hybridization using the partial sequence as a probe.Type: ApplicationFiled: May 24, 2004Publication date: November 18, 2004Applicant: Ajinomoto Co., Inc.Inventors: Kyoko Koibuchi, Hiroaki Nagasaki, Ari Yuasa, Jiro Kataoka, Katsuhiko Kitamoto
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Publication number: 20040225116Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of certain nitriles to the corresponding amides, and the amidase is useful for hydrolysis of certain amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.Type: ApplicationFiled: May 8, 2003Publication date: November 11, 2004Inventors: Mark S. Payne, Robert Dicosimo, John E. Gavagan, Robert D. Fallon
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Publication number: 20040214264Abstract: This invention relates to a nucleic acid encoding a variant human DDAH protein and to said variant DDAH protein as well as a method for screening a subject to determine if said subject is a carrier of a variant gene that encodes said variant DDAH protein. Further this invention relates to a method for detecting or diagnosing a risk of, or predisposition to, cardiovascular disease and diabetes in a subject, for selecting treatment in a subject and for selecting subjects for studies testing cardiovascular and diabetes drugs, a method for the treatment of type 2 diabetes as well as to transgenic animals.Type: ApplicationFiled: April 19, 2002Publication date: October 28, 2004Inventors: Veli-Pekka Valkonen, Jukka T. Salonen, Mia Pirskanen, Tomi-Pekka Tuomainen, Juha Laakso, Reijo Laaksonen
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Publication number: 20040202644Abstract: Staphylococcus aureus peptide deformylase has been crystallized, and the three-dimensional x-ray crystal structure has been solved to 1.9 Å resolution. The x-ray crystal structure is useful for solving the structure of other molecules or molecular complexes, and designing modifiers of peptide deformylase activity.Type: ApplicationFiled: January 10, 2003Publication date: October 14, 2004Inventors: Eric T. Baldwin, Melissa S. Harris
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Publication number: 20040197772Abstract: Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.Type: ApplicationFiled: March 30, 2001Publication date: October 7, 2004Inventors: Sarita Chauhan, Robert Dicosimo, Robert D. Fallon, John E. Gavagan, Mark S. Payne
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Publication number: 20040175799Abstract: A method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes, or a new bacterium Chryseobacterium sp. No. 9670 belonging to the genus Chryseobacterium, and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of the enzyme, and subsequently collecting the enzyme from the culture mixture and a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein, as well as a gene which encodes the enzyme, a recombinant vector which contains the gene, a transformant transformed with the vector and a method in which the transformant is cultured in a medium to effect production of the protein-deamidating enzyme and then the protein-deamidating enzyme is collected from the culture mixture.Type: ApplicationFiled: April 2, 2004Publication date: September 9, 2004Applicant: AMANO ENZYME INC.Inventor: Shotaro Yamaguchi
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Publication number: 20040166564Abstract: The present invention provides the D-aminoacylase-encoding gene derived from Hypomyces mycophilus, a filamentous fungus, the polypeptide encoded by the gene, and the homologues thereof. The D-aminoacylase of the present invention is capable of producing D-tryptophan from N-acetyl-D-tryptophan. D-tryptophan is useful as a medicinal raw material or the like.Type: ApplicationFiled: December 31, 2003Publication date: August 26, 2004Applicant: Daicel Chemical Industries, Ltd., a Japan corporationInventors: Kazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama
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Publication number: 20040106172Abstract: A hydrolyzing/dehydration condensing enzyme having the physical and chemical properties (1) to (3): (1) function and substrate specificity: the enzyme catalyzes a hydrolyzing or dehydration condensing reaction of an amide bond; (2) an optimal temperature range of about 55° C.; and (3) an optimal pH range of 6-8.Type: ApplicationFiled: August 26, 2003Publication date: June 3, 2004Applicant: Okayama UniversityInventors: Kazuhiro Nakanishi, Takaharu Sakiyama, Koreyoshi Imamura
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Publication number: 20040087000Abstract: A three-dimensional structure is described of a complex of isopenicillin N synthase (IPNS) with Fe and its substrate ACV. This structure is used to design modified enzymes IPNS, DAOCS, DACS, DAOC/DACS and other related enzymes of the penicillin and cephalosporin biosynthesis pathway, which modified enzymes may accept unnatural substrates or improve production efficiency or produce improved products. Specific modifications of specific amino acid residues are proposed and exemplified.Type: ApplicationFiled: July 7, 2003Publication date: May 6, 2004Applicant: ISIS INNOVATION LIMITEDInventors: Christopher J. Schofield, Jack E. Baldwin, Ian Clifton, Janos Hajdu, Charles M.H. Hensgens, Peter L. Roach
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Patent number: 6730634Abstract: The invention relates to a method of identifying herbicides and to the use of inhibitors of plant peptide deformylase as broad spectrum herbicides.Type: GrantFiled: July 14, 2000Date of Patent: May 4, 2004Assignee: University of Kentucky Research FoundationInventors: Robert L. Houtz, Lynnette M. A. Dirk, Mark Alan Williams