Lyase (4. ) Patents (Class 435/232)
  • Patent number: 10927364
    Abstract: The present invention relates to polypeptides having carbonic anhydrase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: July 20, 2017
    Date of Patent: February 23, 2021
    Assignee: Novozymes A/S
    Inventors: Sonja Salmon, Martin Simon Borchert, Thomas Holberg Blicher, Wolfgang Streit, Mirjam Perner
  • Patent number: 10705093
    Abstract: Compositions and methods for determining the level of thiol and disulfide containing molecules in a sample are provided. The compositions and methods can be used to determine the level of oxidative stress in a subject with or without antioxidant treatment. Also provided are biomarkers of oxidative stress.
    Type: Grant
    Filed: July 27, 2015
    Date of Patent: July 7, 2020
    Assignee: Bloodworks
    Inventors: Xiaoyun Fu, Shelby A. Cate, Jose Aron Lopez, Junmei Chen, Barbara A. Konkle
  • Patent number: 10662417
    Abstract: The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.
    Type: Grant
    Filed: July 5, 2017
    Date of Patent: May 26, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Rune Nygaard Monrad, Peter Kamp Hansen, Frank Winther Rasmussen, Anne Vindum Due, Jens Erik Nielsen, Garry Paul Gippert
  • Patent number: 10435717
    Abstract: The present invention provides genetically modified eukaryotic host cells that produce isoprenoid precursors or isoprenoid compounds. A subject genetically modified host cell comprises increased activity levels of one or more of mevalonate pathway enzymes, increased levels of prenyltransferase activity, and decreased levels of squalene synthase activity. Methods are provided for the production of an isoprenoid compound or an isoprenoid precursor in a subject genetically modified eukaryotic host cell. The methods generally involve culturing a subject genetically modified host cell under conditions that promote production of high levels of an isoprenoid or isoprenoid precursor compound.
    Type: Grant
    Filed: October 2, 2017
    Date of Patent: October 8, 2019
    Assignee: The Regents of the University of California
    Inventors: Jay D. Keasling, James Kirby, Eric M. Paradise
  • Patent number: 10415028
    Abstract: The present description relates to recombinant or engineered carbonic anhydrase polypeptides, variants, and functional derivatives thereof, having improved properties that make them advantageous for use in CO2 capture operations (e.g., CO2 capture solvents, alkaline pH, and/or elevated temperatures), as well as polynucleotides and vectors encoding same. The present description also relates to methods, processes and systems for CO2 capture which make use of the recombinant or engineered carbonic anhydrase polypeptides, variants, and functional derivatives thereof.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: September 17, 2019
    Assignee: CO2 Solutions Inc.
    Inventors: Normand Voyer, Richard Daigle, Éric Madore, Sylvie Fradette
  • Patent number: 10364418
    Abstract: Described are 3-hydroxyisovalerate (HIV) synthase variants having improved activity in converting acetone and a compound which provides an activated acetyl group into 3-hydroxyisovalerate (HIV). Moreover, described are in particular methods for the production of 3-hydroxyisovalerate and methods for the production of isobutene from acetone utilizing the HIV synthase variants of the present invention.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: July 30, 2019
    Assignees: Global Bioenergies, Scientist of Fortune, S.A.
    Inventors: Philippe Marliére, Marc Delcourt, Sabine Mazaleyrat, Jean-Baptiste Barbaroux
  • Patent number: 10308925
    Abstract: Embodiment of the present invention discloses a kind of methionine lyase and its encoding gene and biosynthetic method. According to the present invention, the gene encoding methionine lyase as shown in SEQ ID No. 1 is separated from the genome of C. rosea. Embodiment of the present invention further provides an efficient biosynthetic method of methionine lyase, comprising: (1) cloning gene (shown in SEQ ID No.1) encoding methionine degradation enzyme into a yeast expression vector to construct recombinant yeast expression vector; (2) transforming the recombinant yeast expression vector into Saccharomyces cerevisiae to obtain expression strain; (3) inducing the expression strain to express the methionine lyase, collecting induced strains, purifying expressed recombinant methionine lyase. Purity of recombinant methionine lyase prepared according to the present invention is above 90%, and its efficiency of degradating methionine can reach 0.53±0.0030 ?M MTL·h?1·mg protein?1.
    Type: Grant
    Filed: December 3, 2015
    Date of Patent: June 4, 2019
    Assignee: HUBEI UNIVERSITY OF TECHNOLOGY
    Inventors: Yajie Tang, Kaizhi Jia, Yanghua Xu, Quan Zhang, Hongmei Li
  • Patent number: 10287568
    Abstract: Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.
    Type: Grant
    Filed: October 1, 2015
    Date of Patent: May 14, 2019
    Assignee: Impossible Foods Inc.
    Inventors: Aniket Kale, Ranjani Varadan, Simon Christopher Davis
  • Patent number: 10131925
    Abstract: There is described a method for producing an ester of 3-hydroxypropionic acid, the method comprising: culturing an Acetobacter lovaniensis bacterium in a growth medium containing phosphate at a level which is more than 1 g/liter, wherein culturing of the bacterium produces the ester of 3-hydroxypropionic acid.
    Type: Grant
    Filed: February 6, 2015
    Date of Patent: November 20, 2018
    Assignee: VERDANT BIOPRODUCTS LIMITED
    Inventor: Irene Finnegan
  • Patent number: 10093913
    Abstract: Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.
    Type: Grant
    Filed: July 27, 2017
    Date of Patent: October 9, 2018
    Assignee: Impossible Foods Inc.
    Inventors: Aniket Kale, Ranjani Varadan, Simon Christopher Davis
  • Patent number: 10091984
    Abstract: Methods and compositions for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, the biological sample including at least one circulating tumor cell from the subject. The methods may include a step of contacting the biological sample with a protective agent composition that includes a preservative agent, an optional anticoagulant, and a quenching agent to form a mixture that includes the protective agent composition and the sample.
    Type: Grant
    Filed: July 22, 2014
    Date of Patent: October 9, 2018
    Assignee: STRECK, INC.
    Inventors: Rohan M. Fernando, Wayne L. Ryan, Brad Hunsley
  • Patent number: 10093912
    Abstract: Provided is a novel improved nitrile hydratase with improved resistance to amide compounds under high temperatures. Specifically provided is a nitrile hydratase having at least one amino acid mutation selected from (a) to (d) below, in the amino acid sequence expressed in SEQ ID NO:50 (X1 to X27 represent independent arbitrarily-defined amino acid residuals). (a) X1 is valine or glycine (b) X9 is valine or threonine (c) X23 is an amino acid selected from a group consisting of isoleucine, leucine, methionine and threonine, (d) X24 is leucine.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: October 9, 2018
    Assignee: Mitsubishi Chemical Corporation
    Inventors: Aya Kitahara, Takanori Akiyama, Miki Wakamatsu, Fumiaki Watanabe
  • Patent number: 10087434
    Abstract: Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.
    Type: Grant
    Filed: July 27, 2017
    Date of Patent: October 2, 2018
    Assignee: Impossible Foods Inc.
    Inventors: Aniket Kale, Ranjani Varadan, Simon Christopher Davis
  • Patent number: 10006019
    Abstract: A novel method for processing soluble plant leaf proteins is described. While leaf proteins are considered potentially the most abundant source of protein in nature, the lack of efficient processing techniques for leaf proteins has limited their commercial use. The method described in this patent provides a means of extracting and purifying leaf proteins from plants which is suitable for leaf protein production on an industrial scale.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: June 26, 2018
    Assignee: University of Maryland, College Park
    Inventors: Yangming Martin Lo, Hong Fu
  • Patent number: 9994878
    Abstract: Bacteria are genetically engineered to produce 3-hydroxypropionate (3-HP). The bacteria are carboxydotrophic acetogens. The bacteria produce acetyl-coA using the Wood-Ljungdahl pathway for fixing CO/CO2. A malonyl-coA reductase from a bacterium that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced The production of 3-HP can be improved by overproduction of acetyl-CoA carboxylase or by overproduction of biotin. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient.
    Type: Grant
    Filed: May 30, 2013
    Date of Patent: June 12, 2018
    Assignee: LanzaTech New Zealand Limited
    Inventors: Michael Koepke, Wendy Yiting Chen
  • Patent number: 9868970
    Abstract: The invention provides an effective method for improving N-acetylglucosamine (GlcNAc) production by engineered B. subtilis Deletion of phosphoenolpyruvate carboxykinase encoding gene pckA and encoding pyruvate kinase gene pyK in recombinant GlcNAc-producing strain BSGNK-PxylA-glmS-P43-GNA1 (BSGNK) is first performed to enhance GlcNAc production, followed by overexpression of pyruvate carboxylase encoding gene pycA for facilitating cell growth. Finally, the GlcNAc production of the recombinant strain BPTS3 reached to 11.3 g/L, which was 1.84-fold of BSGNK. This method can be used for improve cellular property of engineered B. subtilis for GlcNAc production, which can be further applied to industrial production of GlcNAc.
    Type: Grant
    Filed: July 8, 2016
    Date of Patent: January 16, 2018
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Long Liu, Jian Chen, Guocheng Du, Jianghua Li, Yang Gu, Yang Song, Jieying Deng, Yawen Zhao
  • Patent number: 9809829
    Abstract: The present invention provides genetically modified eukaryotic host cells that produce isoprenoid precursors or isoprenoid compounds. A subject genetically modified host cell comprises increased activity levels of one or more of mevalonate pathway enzymes, increased levels of prenyltransferase activity, and decreased levels of squalene synthase activity. Methods are provided for the production of an isoprenoid compound or an isoprenoid precursor in a subject genetically modified eukaryotic host cell. The methods generally involve culturing a subject genetically modified host cell under conditions that promote production of high levels of an isoprenoid or isoprenoid precursor compound.
    Type: Grant
    Filed: August 4, 2014
    Date of Patent: November 7, 2017
    Assignee: The Regents of the University of California
    Inventors: Jay D. Keasling, James Kirby, Eric M. Paradise
  • Patent number: 9707333
    Abstract: The invention described herein teaches methods of removing microvesicular particles, which include but are not limited to exosomes, from the systemic circulation of a subject in need thereof with the goal of reversing antigen-specific and antigen-nonspecific immune suppression. Said microvesicular particles could be generated by host cells that have been reprogrammed by neoplastic tissue, or the neoplastic tissue itself. Compositions of matter, medical devices, and novel utilities of existing medical devices are disclosed.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: July 18, 2017
    Assignee: Aethlon Medical, Inc.
    Inventors: Thomas Ichim, Richard H. Tullis
  • Patent number: 9605252
    Abstract: The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: March 28, 2017
    Assignee: Codexis, Inc.
    Inventors: Gjalt W. Huisman, Nicholas J. Agard, David Elgart, Xiyun Zhang
  • Patent number: 9564356
    Abstract: A technique includes applying a liquid dielectric composition onto a substrate, where the composition includes metal ions, at least partially curing the composition to form a dielectric layer with the metal ions, patterning the dielectric layer to form electron-rich regions at a surface thereof, heating the patterned dielectric layer to drive the metal ions to the electron-rich regions thereof, thereby forming a metal barrier layer on at least a portion of the surface of the dielectric layer, and depositing one or more metal layers onto the metal barrier layer.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: February 7, 2017
    Assignee: International Business Machines Corporation
    Inventors: Vaughn R. Deline, Geraud J. Dubois, Willi Volksen
  • Patent number: 9546372
    Abstract: The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in Nannochloropsis and other microalgae.
    Type: Grant
    Filed: June 11, 2013
    Date of Patent: January 17, 2017
    Assignee: Synthetic Genomics, Inc.
    Inventors: Michele M. Champagne, Toby H. Richardson, Jun Urano
  • Patent number: 9447385
    Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
    Type: Grant
    Filed: February 13, 2014
    Date of Patent: September 20, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
  • Patent number: 9441238
    Abstract: Methods and materials useful for modulating the sensitivity of cells to an inhibitor of acetohydroxyacid synthase (AHAS) are disclosed. For example, nucleic acid molecules encoding AHAS large subunits are disclosed as well as methods for using such nucleic acid molecules to transform microbial cells and plant cells, and to confer modulated sensitivity to AHAS-inhibiting compounds onto such cells. Further provided are materials and methods useful for modulating growth, development, activity, and characteristics of host cells and organisms.
    Type: Grant
    Filed: April 26, 2013
    Date of Patent: September 13, 2016
    Assignee: Synthetic Genomics, Inc.
    Inventors: Michele M. Champagne, Jun Urano
  • Patent number: 9376659
    Abstract: Disclosed is a yeast/polyphenol ferment extract, and a personal care composition including the yeast/polyphenol ferment extract. Also disclosed is a method for preparing and using the yeast/polyphenol extract and the personal care composition.
    Type: Grant
    Filed: July 23, 2009
    Date of Patent: June 28, 2016
    Assignee: ARCH PERSONAL CARE PRODUCTS, L.P.
    Inventors: Smitha Rao, James Vincent Gruber, Geoffrey Jamison Brooks
  • Patent number: 9371537
    Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an SGT1 polypeptide, or a CLC-pKG polypeptide, or a HD-hydrolase-like polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an SGT1 polypeptide, or a CLC-pKG polypeptide, or a HD-hydrolase-like polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.
    Type: Grant
    Filed: September 24, 2010
    Date of Patent: June 21, 2016
    Assignees: BASF Plant Science Company GmbH, Crop Functional Genomics Center
    Inventors: Yang Do Choi, Doil Choi, Christophe Reuzeau, Ji-Young Song, Youn-Il Park
  • Patent number: 9365862
    Abstract: This disclosure provides for four different sorghum mutants that exhibit resistance to ALS-inhibiting herbicides. This disclosure also provides for methods of using such sorghum mutants that exhibit resistance to ALS-inhibiting herbicides in breeding methods to make sorghum hybrids, varieties, or lines. The sorghum hybrids, varieties, and lines provided in this disclosure can be used in methods of controlling weeds.
    Type: Grant
    Filed: May 25, 2012
    Date of Patent: June 14, 2016
    Assignee: NUtech Ventures
    Inventor: Ismail M. Dweikat
  • Patent number: 9321806
    Abstract: A novel method for processing soluble plant leaf proteins is described. While leaf proteins are considered potentially the most abundant source of protein in nature, the lack of efficient processing techniques for leaf proteins has limited their commercial use. The method described in this patent provides a means of extracting and purifying leaf proteins from plants which is suitable for leaf protein production on an industrial scale.
    Type: Grant
    Filed: May 15, 2008
    Date of Patent: April 26, 2016
    Assignee: University of Maryland, College Park
    Inventors: Martin Lo, Hong Fu
  • Patent number: 9284546
    Abstract: Human cystathionine ?-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine ?-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologues are described. In addition, a method to express and purify recombinant human cystathionine ?-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: March 15, 2016
    Assignee: THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE
    Inventor: Jan P. Kraus
  • Patent number: 9051586
    Abstract: The present invention relates to a technique for capturing carbon dioxide and converting the carbon dioxide to carbonate minerals using a recombinant whole cell biocatalyst expressing carbonic anhydrase. More particularly, the present invention relates to a composition for capturing carbon dioxide and a method for capturing carbon dioxide using the composition, which composition comprises a whole cell of a transformant formed with a vector including a nucleic acid encoding a recombinant carbonic anhydrase; a cell lysate or its fraction of the whole cell; or a recombinant carbonic anhydrase isolated from the whole cell. Further, the present invention relates to a composition and method for converting the carbon dioxide to carbonate minerals using the carbon dioxide capturing composition.
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: June 9, 2015
    Assignee: POSTECH ACADEMY-INDUSTRY FOUNDATION
    Inventors: Hyung Joon Cha, Im Gyu Kim, Dong Gyun Kang, Byung Hoon Jo, Jeong Hyun Seo
  • Publication number: 20150147780
    Abstract: Disclosed herein are embodiments for a novel method of producing an organic compound, including harvesting at least one organic compound from an organism or cell line genetically engineered with a gene for at least one proton-pump protein.
    Type: Application
    Filed: November 24, 2014
    Publication date: May 28, 2015
    Inventors: Eugene Dinescu, Vincent Dinescu
  • Publication number: 20150147783
    Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.
    Type: Application
    Filed: February 9, 2015
    Publication date: May 28, 2015
    Inventors: Steen Troels Joergensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
  • Publication number: 20150147800
    Abstract: The present invention relates to a method for the enzymatic decarboxylation of malonic acid (propanedioic acid) derivatives catalyzed by enzymes structurally and/or functionally related to arylmalonate decarboxylase (AMDase) as isolated from microorganisms of the genus Bordetella. The present invention also relates to novel enzymes with a decarboxylase activity, useful for performing the claimed method, mutants thereof, corresponding coding sequences and expression systems, methods of preparing said novel enzymes, and screening methods for obtaining further suitable enzymes also having said decarboxylase activity.
    Type: Application
    Filed: December 29, 2014
    Publication date: May 28, 2015
    Inventors: Bernhard Hauer, Nina Schneider, Krzysztof Okrasa, Jason Micklefield, David Leys, Colin Levy
  • Patent number: 9040282
    Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing a dicarboxylic acid (diacid). Such diacids include diketide-diacids and triketide-diacids. The invention includes recombinant nucleic acid encoding the PKS, and host cells comprising the PKS. The invention also includes methods for producing the diacids.
    Type: Grant
    Filed: September 26, 2013
    Date of Patent: May 26, 2015
    Assignee: The Regents of the University of California
    Inventors: Leonard Katz, Jeffrey L Fortman, Jay D Keasling
  • Publication number: 20150141714
    Abstract: The disclosed invention provides methods and compositions for increasing terpenoid production, such as sesquiterpenoids, such as farnesene, in plant cells.
    Type: Application
    Filed: January 14, 2013
    Publication date: May 21, 2015
    Inventors: Joshua Blakeslee, Katrina Cornish, Oswald Crasta, Otto Folkerts, Dave Jessen, Ramesh Nair
  • Publication number: 20150140631
    Abstract: Described is a method for generating conjugated dienes through a biological process. More specifically, the application describes a method for producing conjugated dienes (for example butadiene, isoprene or dimethylbutadiene) from light alkenols via enzymatic dehydration, in particular by making use of an alkenol dehydratase.
    Type: Application
    Filed: January 28, 2015
    Publication date: May 21, 2015
    Inventor: Philippe Marliere
  • Publication number: 20150139975
    Abstract: The present invention relates to recombinant factor H and variants and conjugates thereof and methods of their production, as well as uses and methods of treatment involving the materials.
    Type: Application
    Filed: November 14, 2014
    Publication date: May 21, 2015
    Inventors: Christoph SCHMIDT, Paul N. BARLOW, Anna RICHARDS
  • Patent number: 9034629
    Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described.
    Type: Grant
    Filed: January 27, 2014
    Date of Patent: May 19, 2015
    Assignee: Joule Unlimited Technologies, Inc.
    Inventors: Frank Anthony Skraly, Ning Li
  • Patent number: 9034318
    Abstract: The invention provides reagents and methods for enzyme replacement therapy using chemically modified species of human cystathionine ?-synthase (CBS) to treat homocystinuria and other related diseases and disorders.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: May 19, 2015
    Assignee: THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE
    Inventors: Jan P Kraus, Tomas Majtan, Erez Bublil
  • Publication number: 20150132808
    Abstract: A mutant lysine decarboxylase is produced by replacing at least one of the amino-acids in the amino acid sequence shown in sequence ID No. 4 of the sequence listing with another amino-acid.
    Type: Application
    Filed: January 17, 2013
    Publication date: May 14, 2015
    Inventors: Daisuke Mochizuki, Tadashi Araki, Akiko Natsuji, Tomomi Sakai, Tomonori Hidesaki
  • Patent number: 9023627
    Abstract: Systems, compounds and methods for the conversion of C1 carbon compounds to higher carbon compounds useful for the generation of commodity compounds.
    Type: Grant
    Filed: September 15, 2011
    Date of Patent: May 5, 2015
    Assignee: University of Washington through its Center for Commercialization
    Inventors: Justin Siegel, David Baker, Amanda Lee Smith, Mary E. Lidstrom, Catherine Louw
  • Publication number: 20150118725
    Abstract: Described is a method for generating isoprenol through a biological process. More specifically, described is a method for producing isoprenol from mevalonate.
    Type: Application
    Filed: April 4, 2013
    Publication date: April 30, 2015
    Inventors: Marc Delcourt, Maria Anissimova, Philippe Marliere
  • Publication number: 20150110766
    Abstract: A potent complement regulator is disclosed. The complement regulator comprises a complement regulatory region connected by a flexible linker to a multifunctional binding region that enables binding to C3b activation/inactivation products and/or oxidation end products, as well as to polyanionic surface markers on host cells. An embodiment of the invention utilizes factor H SCRs 1-4 as the complement regulatory region and factor H SCRs 19 and 20 as the multi-functional binding region, linked together by a poly-Gly linker at least 12 residues in length. Pharmaceutical compositions comprising the complement regulator and methods of using the complement regulator are also disclosed.
    Type: Application
    Filed: March 15, 2013
    Publication date: April 23, 2015
    Applicant: UNIVERSITY ULM
    Inventors: John D. Lambris, Christoph Schmidt, Daniel Ricklin
  • Publication number: 20150111278
    Abstract: The present invention relates to use of Caminibacter carbonic anhydrase in CO2 extraction, e.g., from flue gas, natural gas, biogas or ambient air. The Caminibacter carbonic anhydrases are especially well suited for these purpose due to their extreme thermostability.
    Type: Application
    Filed: December 17, 2014
    Publication date: April 23, 2015
    Inventors: Martin Borchert, Paria Saunders
  • Patent number: 9011844
    Abstract: Human cystathionine ?-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine ?-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologs are described. In addition, a method to express and purify recombinant human cystathionine ?-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: April 21, 2015
    Assignee: The Regents of the University of Colorado, a Body Corporate
    Inventor: Jan P. Kraus
  • Publication number: 20150106971
    Abstract: There is disclosed a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a threonine synthase and having at least 90% sequence identity to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 or at least 87% sequence identity to SEQ ID NO:4, or SEQ ID NO:5; (ii) a polypeptide encoded by any one of said polynucleotides set forth in (i); or (iii) a polypeptide having at least 95% sequence identity to SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8; or (iv) a construct, vector or expression vector comprising the polynucleotide as set forth in (i).
    Type: Application
    Filed: August 31, 2012
    Publication date: April 16, 2015
    Applicant: PHILIP MORRIS PRODUCTS S.A.
    Inventors: Lucien Bovet, Nicolas Sierro
  • Publication number: 20150104850
    Abstract: A method of improving the folding of an enzyme comprising a thiamine pyrophosphate (TPP) binding domain, the method comprising: providing a nucleic acid encoding the enzyme comprising a TPP binding domain, in which one or more of the TPP binding domains in the enzyme monomer are replaced with a TPP binding domain from a thermostable TPP-binding protein, and expressing the nucleic acid under conditions that allow expression and folding of the enzyme. The enzyme may be pyruvate decarboxylase.
    Type: Application
    Filed: May 20, 2013
    Publication date: April 16, 2015
    Applicant: ReBioTechnologies Limted
    Inventors: David Jonathan Leak, Jeremy Bartosiak-Jentys
  • Patent number: 9005950
    Abstract: The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: April 14, 2015
    Assignee: Novozymes A/S
    Inventors: Sanne Schoeder Glad, Carsten Andersen, Torben Vedel Borchert, Katja Salomon Johansen, Henrik Frisner, Mads Eskelund Bjornvad, Thomas Thisted
  • Patent number: 9005939
    Abstract: The present invention relates to an isolated, recombinant or synthetic polynucleotide encoding a polypeptide with protoilludene synthase activity and comprising a sequence selected from the group consisting of a) SEQ ID Nos. 1 or 14 of the attached sequence listing; b) a nucleic acid sequence complementary to SEQ ID Nos. 1 or 14; c) nucleic acid sequences which hybridize under stringent conditions to the nucleic acid sequences defined in a) and b) or their complementary strands, as well as to the polypeptide encoded by the isolated polynucleotide, as well as a method for the production of melleolides employing the polynucleotide or polypeptide of the invention.
    Type: Grant
    Filed: January 31, 2013
    Date of Patent: April 14, 2015
    Assignee: Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
    Inventors: Stefan Jennewein, Benedikt Engels, Torsten Grothe, Marc Stadler
  • Publication number: 20150099283
    Abstract: A method of producing patchoulol and 7-epi-?-selinene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). The method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-?-selinene, compounds which can be useful in the field of perfumery.
    Type: Application
    Filed: December 19, 2014
    Publication date: April 9, 2015
    Applicant: FIRMENICH SA
    Inventors: Michel SCHALK, Fabienne DEGUERRY
  • Publication number: 20150101084
    Abstract: The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.
    Type: Application
    Filed: December 15, 2014
    Publication date: April 9, 2015
    Applicant: Keygene N.V.
    Inventors: Robert Cornelis SCHUURINK, Michael Albertus Haring, Petronella Martina Bleeker