Isomerase (5. ) Patents (Class 435/233)
-
Patent number: 7915025Abstract: The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.Type: GrantFiled: May 4, 2007Date of Patent: March 29, 2011Assignees: The Scripps Research Institute, The Regents of the University of CaliforniaInventors: Peter Schultz, Lei Wang, John Christopher Anderson, Jason W. Chin, David R. Liu, Thomas J. Magliery, Eric Meggers, Ryan Aaron Mehl, Miro Pastrnak, Stephen William Santoro, Zhiwen Zhang
-
Publication number: 20110053245Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-, arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.Type: ApplicationFiled: December 22, 2008Publication date: March 3, 2011Applicant: VERENIUM CORPORATIONInventors: David P. Weiner, Peter Luginbuhl, Analia Bueno, Joslin M. Cuenca, Mervyn L. De Souza, Sherry Kollmann
-
Publication number: 20110045573Abstract: Alanine 2,3-aminomutase sequences are disclosed, as are cells having alanine 2,3-aminomutase activity and methods of selecting for such cells. Methods for producing beta-alanine, pantothenate, 3-hydroxypropionic acid, as well as other organic compounds, are disclosed.Type: ApplicationFiled: November 4, 2010Publication date: February 24, 2011Inventors: Hans H. Liao, Ravi R. Gokarn, Steven J. Gort, Holly J. Jessen, Olga Selifonova
-
Patent number: 7883882Abstract: Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.Type: GrantFiled: November 30, 2009Date of Patent: February 8, 2011Assignee: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
-
Publication number: 20110020895Abstract: This disclosure provides crystalline flavonoid or flavanone isomerases, isolated non-native isomerase having the structural coordinates of said crystalline isomerase, and nucleic acids encoding such non-native isomerase. Also disclosed are methods of predicting the activity and/or substrate specificity of a putative isomerase, methods of identifying potential ismerase substrates, and methods of identifying potential isomerase inhibitors.Type: ApplicationFiled: September 3, 2010Publication date: January 27, 2011Inventors: Joseph P. Noel, Joseph M. Jez, Marianne E. Bowman
-
Publication number: 20110020868Abstract: Compositions and methods for improving expression and/or secretion of a polypeptide of interest in a host cell are provided. Compositions including a coding sequence for a bacterial secretion signal peptide are provided. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. The present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.Type: ApplicationFiled: September 16, 2010Publication date: January 27, 2011Applicant: PFENEX, INC.Inventors: Russell J. Coleman, Diane Retallack, Stacey L. Lee, Charles D. Hershberger
-
Publication number: 20110014677Abstract: A DNA fragment containing a gene which encodes a specific gene regulatory region alone or the gene regulatory region together with a signal peptide; a recombinant vector containing the DNA fragment; a transformant containing the recombinant vector; and a method of producing a recombinant protein by using the transformant. According to the invention, it is possible to produce a protein in a large amount at a high efficiency regardless of the kind of the recombinant protein.Type: ApplicationFiled: July 9, 2008Publication date: January 20, 2011Inventors: Yuji Hatada, Yukari Ohta, Yuko Hidaka, Nobuyuki Nakamura
-
Publication number: 20110008867Abstract: The invention features compositions and methods that are useful for the production of a compound.Type: ApplicationFiled: December 22, 2009Publication date: January 13, 2011Applicant: GREENLIGHT BIOSCIENCESInventors: Andrey J. Zarur, James Swartz
-
Publication number: 20110003365Abstract: Provided is a method of preparing an induced pluripotent stem cell (iPS cell) deprived of a reprogramming gene, including providing an iPS cell having an expression vector wherein a loxP sequence is placed on each of the 5? and 3? sides of the reprogramming gene or a vector component necessary for the replication of the reprogramming gene in the same orientation, and treating the IPS cell with Cre recombinase. Also provided are an iPS cell deprived of a reprogramming gene, as obtained by the method, and a use of the iPS cell as a cell source for producing somatic cells.Type: ApplicationFiled: May 28, 2010Publication date: January 6, 2011Applicant: KYOTO UNIVERSITYInventors: Shinya YAMANAKA, Keisuke OKITA
-
Publication number: 20100323420Abstract: We have developed a general procedure for the regulated (inducible) dimerization or oligomerization of intracellular proteins and disclose methods and materials for using that procedure to regulatably initiate cell-specific apoptosis (programmed cell death) in genetically engineered cells.Type: ApplicationFiled: January 7, 2010Publication date: December 23, 2010Inventors: Gerald Crabtree, Stuart Schreiber, David Spencer, Thomas Wandless, Peter Belshaw
-
Patent number: 7846707Abstract: Alanine 2,3-aminomutase sequences are disclosed, as are cells having alanine 2,3-aminomutase activity and methods of selecting for such cells. Methods for producing beta-alanine, pantothenate, 3-hydroxypropionic acid, as well as other organic compounds, are disclosed.Type: GrantFiled: December 21, 2009Date of Patent: December 7, 2010Assignee: Novozymes A/SInventors: Hans H. Liao, Ravi R. Gokarn, Steven J. Gort, Holly J. Jessen, Olga Selifonova
-
Publication number: 20100285540Abstract: A process for producing (R)-?-arylalanines is described. The process converts (S)-?-arylalanine to (R)-?-arylalanine using phenylalanine aminomutase and amino acid racemase. The (R)-?-arylalanine product can be used to form pharmaceutically useful drugs. Also disclosed are DNA sequences encloding phenylalanine aminomutase and amino acid racemase enzymes, sequences for PCR primers of the DNA sequences, and sequences for expressed aminomutase and amino acid racemase enzymes.Type: ApplicationFiled: April 30, 2010Publication date: November 11, 2010Applicant: Board of Trustees of Michigan State UniversityInventors: Kevin D. Walker, Brad M. Cox
-
Patent number: 7829311Abstract: An acid-resistant peptide solubility tag (an “inclusion body tag”) is provided that is effective in producing peptides of interest in an insoluble form. Fusion peptide constructs comprising the inclusion body tag fused to a peptide of interest are provided. An acid cleavable peptide moiety separates the inclusion body tag from the peptide of interest so that acid hydrolysis can be used during subsequent processing steps to separate the tag from the desired peptide of interest. The present inclusion body tag's resistance to acid hydrolysis facilitates easier and cleaner separation of the peptide of interest after acid hydrolysis. Specifically, a ketosteroid isomerase-derived inclusion body tag is provided that has been engineered to be more resistant to acid hydrolysis.Type: GrantFiled: July 14, 2008Date of Patent: November 9, 2010Assignee: E.I. du Pont de Nemours and CompanyInventors: Linda Jane DeCarolis, Stephen R. Fahnestock
-
Publication number: 20100267115Abstract: Methods of using beta-alanine/pyruvate aminotransferase to produce 3-hydroxypropionic acid and derivatives thereof, from beta-alanine, are disclosed. Cells and recombinant nucleic acids that can be used to practice the methods are also disclosed.Type: ApplicationFiled: June 29, 2010Publication date: October 21, 2010Inventors: Hans H. Liao, Ravi R. Gokarn, Steven John Gort, Holly Jean Jessen, Olga V. Selifonova
-
Publication number: 20100261253Abstract: The invention is directed to a novel purified mouse C5-epimerase, fragments thereof, nucleic acids encoding the same and the recombinant production thereof. The invention is also directed to fragments of such epimerase, especially N-terminal fragments that are useful in fusion protein constructs to enhance the activity of recombinantly-produced heterologous epimerase enzymes.Type: ApplicationFiled: July 8, 2008Publication date: October 14, 2010Applicants: BioTie Therapies Corp., Inalco S.p.A.Inventors: Markku Jalkanen, Kamel El Darwish, Ulf Lindahl, Jin-Ping Li
-
Patent number: 7807808Abstract: The invention relates to bacteria that have increased levels of protein secretion due to genetic modification, to nucleotide sequences and gene structures containing at least one gene coding for a SecA protein having increased levels of protein secretion, to a SecA having increased levels of protein secretion, and to a method for producing desired proteins using the inventive bacteria. The invention also relates to nucleic acids coding for a SecA protein having increased levels of protein secretion, containing a SecA gene sequence or allele, a SecA homologue or derivative, or nucleotide sequences hybridising therewith and comprising at least one mutation. Surprisingly, just one mutation in a nucleotide of a SecA gene leads to increased levels of protein secretion or to protein secretion for the first time.Type: GrantFiled: November 9, 2009Date of Patent: October 5, 2010Assignee: Danisco US Inc.Inventors: Oliver Koberling, Roland Freudl
-
Publication number: 20100249220Abstract: A chimeric transactivator comprises a transcription activation domain, a repressor protein DNA binding domain and the bacterial DNA gyrase B subunit. A target gene is operatively linked to operator DNA sequences recognized by the repressor binding domain. The addition of the antibiotic coumermycin results in a coumermycin-switched dimerization of the transactivator, which then binds to operator DNA sequences and activates transcription of the target gene. The addition of novobiocin switches off expression of the target gene by abolishing coumermycin-induced dimerization of the transactivator.Type: ApplicationFiled: April 30, 2010Publication date: September 30, 2010Inventors: Hui-Fen ZHAO, Shi-Hsiang Shen
-
Publication number: 20100248312Abstract: The present disclosure relates generally to polypeptides having improved alanine 2,3-aminomutase (AAM) activity, the polynucleotides encoding the AAM polypeptides, and expression vectors and host cells for expressing the AAM polypeptides.Type: ApplicationFiled: October 16, 2006Publication date: September 30, 2010Applicant: Codexis, Inc.Inventors: Svetlana Balatskaya, Birthe Borup, Ranjini Chatterjee, Ish Dhawan, Richard J. Fox, Kenneth W. Mitchell, Emily C. Mundorff, Les Partridge, Matthew Tobin
-
Patent number: 7792645Abstract: This disclosure provides crystalline flavonoid or flavanone isomerases, isolated non-native isomerase having the structural coordinates of said crystalline isomerase, and nucleic acids encoding such non-native isomerase. Also disclosed are methods of predicting the activity and/or substrate specificity of a putative isomerase, methods of identifying potential isomerase substrates, and methods of identifying potential isomerase inhibitors.Type: GrantFiled: August 29, 2001Date of Patent: September 7, 2010Assignee: The Salk Institute for Biological StudiesInventors: Joseph P. Noel, Joseph M. Jez, Marianne E. Bowman
-
Publication number: 20100216212Abstract: An anti-obesity agent containing, as an active ingredient, a microorganism which belongs to the species Lactobacillus reuteri and is capable of producing lipases having the amino acid sequences respectively depicted in SEQ ID NO: 1, 3 or 5 or amino acid sequences having deletion, substitution or addition of one or more amino acids in the amino acid sequences respectively depicted in SEQ ID NO: 1, 3 or 5. The anti-obesity agent enables a patient to take a normal meal yet preventing the absorption of a fat into the body which is the primary cause of obesity.Type: ApplicationFiled: July 25, 2006Publication date: August 26, 2010Applicant: SCHOOL CORP., AZABU VETERIN MED. EDUCA'L INSTITUInventors: Hidetoshi Morita, Toshio Masaoka, Takehito Suzuki
-
Publication number: 20100190225Abstract: Provided is a method of producing D-psicose using a D-psicose epimerase derived from Agrobacterium tumefaciens. Provided are a protein having an amino acid sequence of SEQ ID NO:1 and having a psicose 3-epimerase activity, a gene encoding the protein, a recombinant expression vector containing the gene, and a method of producing D-psicose by reacting the protein produced on a mass scale with D-fructose. The method of producing D-psicose is an environmentally friendly method using a new enzyme, in which an inexpensive substrate is used, and the activity of the enzyme can be retained for a prolonged time period. Thus, the method can be efficiently used for the mass production of D-psicose.Type: ApplicationFiled: May 30, 2006Publication date: July 29, 2010Applicant: CJ CHEILJEDANG CORP.Inventors: Deok-Kun Oh, Hye-Jung Kim, Yong-Joo Lee, Sang-Hoon Song, Seung-Won Park, Jung-Hoon Kim, Seong-Bo Kim
-
Publication number: 20100183578Abstract: Specific nitroprotein biomarkers may be used as prognostic and diagnostic tools for COPD. Identification of certain specific nitroprotein biomarkers allows the development of targeted therapies aimed at prevention and treatment of COPD.Type: ApplicationFiled: December 29, 2009Publication date: July 22, 2010Applicant: Chiesi Farmaceutici S.p.A.Inventors: Dominic M. DESIDERIO, Xianquan Zhan
-
Publication number: 20100184142Abstract: The invention relates to bacteria that have increased levels of protein secretion due to genetic modification, to nucleotide sequences and gene structures containing at least one gene coding for a SecA protein having increased levels of protein secretion, to a SecA having increased levels of protein secretion, and to a method for producing desired proteins using the inventive bacteria. The invention also relates to nucleic acids coding for a SecA protein having increased levels of protein secretion and containing a gene sequence SecA or an allele, homologue or derivative of said nucleotide sequences or nucleotide sequences hybridising therewith and comprising at least one mutation. Surprisingly, just one mutation in a nucleotide of a SecA gene leads to increased levels of protein secretion or to protein secretion for the first time.Type: ApplicationFiled: November 9, 2009Publication date: July 22, 2010Inventors: Oliver Koberling, Roland Freudl
-
Publication number: 20100172881Abstract: The present invention is directed to a method for preparing an expression vector encoding a tailored recombinase, wherein said tailored recombinase recombines asymmetric target sites within the LTR of proviral DNA of a retrovirus inserted into the genome of a host cell and is useful as means for excising the provirus from the genome of the host cell. The present invention further relates to an in vitro-method of optimising the treatment of a retroviral infection of a subject and to the use of tailored recombinases for the preparation of pharmaceutical compositions for reducing the viral load in a subjected infected by a retrovirus.Type: ApplicationFiled: January 3, 2008Publication date: July 8, 2010Applicants: MAX-PLANCK-GESELLSCHAFT zur FORDERUNG der WISSENSCHAFTEN E.V., TECHNISCHE UNIVERSITAT DRESDENInventors: Joachim Hauber, Frank Buchholz, Ilona Hauber, Francis A. Stewart, Indrani Sarkar
-
Publication number: 20100173366Abstract: The invention concerns identification of a gene encoding a novel L-arabinose isomerase of the Bacillus stearothernivphilus strain US 100 (L-AI US 100), a L-arabinose isomerase expressed from said gene, recombinant vectors harbouring said gene, microorganisms transformed with said vector, a protocol for preparing and purifying said recombinant protein, biochemical and kinetic characterization of said recombinant enzyme and a method for bioconversion of a D-galactose solution into a solution rich in D-tagatose using said polypeptide. This novel protein has original characteristics, in particular its independence from metal ions for its activity and its low need for such ions for its thermostability, as well as its potential for isomerizing D-galactose into D-tagatose with great efficacy of about 48% after 7 hours at 70° C.Type: ApplicationFiled: June 28, 2005Publication date: July 8, 2010Applicant: CENTRE OF BIOTECHNOLOGY OF SAFXInventors: Moez Rhimi, Hichem Chouayekh, Mamdouh Ben Ali, Belgacem Naili, Sonia Jemli, Samir Bejar
-
Publication number: 20100169988Abstract: A novel method of identifying and obtaining molecules interacting with neurodegenerative, neurological or neuropsychiatric disorder-associated proteins is provided, which is suitable for drug screening and drug development. Furthermore, drugs and drug targets for the therapeutic intervention of neurodegenerative, neurological or neuropsychiatric disorders, in particular Alzheimer's disease are described.Type: ApplicationFiled: December 6, 2007Publication date: July 1, 2010Inventors: Bernhard Kohli, Uwe Konietzko, Roger Nitsch, Jan Grimm
-
Publication number: 20100151548Abstract: The present invention relates to novel expression cassettes and expression vectors, comprising three nucleic acid sequences for araA, araB and araD, each coding for a polypeptide of an L-arabinose metabolic pathway, in particular, a bacterial L-arabinose metabolic pathway. The invention particularly relates to expression cassettes and expression vectors, comprising codon-optimised nucleic acid sequences for araA, araB and araD. The invention further relates to host cells, in particular modified yeast strains containing the expression cassettes or expression vectors and expressing the polypeptides for the L-arabinose metabolic pathway, in particular, for the bacterial L-arabinose metabolic pathway. When using these modified host cells, arabinose is more effectively fermented by these cells, in particular into ethanol. The present invention is therefore relevant, inter alia, in connection with the production of biochemicals from biomass, such as bioethanol for example.Type: ApplicationFiled: March 20, 2008Publication date: June 17, 2010Inventors: Eckhard Boles, Beate Wiedemann
-
Patent number: 7732161Abstract: The invention provides a biological method of producing isoprenoids.Type: GrantFiled: May 24, 2007Date of Patent: June 8, 2010Assignee: Bio-Technical Resources, a division of Arkion Life Sciences, LLCInventors: James R. Millis, Julie Maurina-Brunker, Thomas W. McMullin
-
Publication number: 20100129865Abstract: An object of the present invention is to provide a novel ketose 3-epimerase, a process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, and a process for producing a ketose by using the enzyme. The present invention solves the above objects by providing a ketose 3-epimerase which is obtainable from a microorganism of the genus Rhizobium, a process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, and a process for converting D- or L-ketohexose into corresponding D- or L-ketohexose by epimerizing the hydroxyl group at the C-3 position of the D- or L-ketohexose; and D- or L-ketopentose into corresponding D- or L-ketopentose by epimerizing the hydroxyl group at the C-3 position of the D- or L-ketopentose; by using the enzyme.Type: ApplicationFiled: November 6, 2006Publication date: May 27, 2010Inventors: Kazuhiko Maruta, Kozo Yamamoto, Tomoyuki Nishimoto, Hiroto Chaen, Tetsuya Nakada
-
Patent number: 7713724Abstract: The invention relates to isolated polypeptides with hydantoin recemase activity, that do not suffer from substrate inhibition. Such polypeptides arc for instance isolated polypeptides with at least 87% identity with SEQ ID: NO. 2 or SEQ ED: NO. 4. The invention also relates to nucleic acid sequences encoding these polypeptides. The invention also relates to processes for the racemisation of enantiomerically enriched hydantoin compounds and to processes for the preparation of enantiomarically enriched I)-or I-? amino acids.Type: GrantFiled: May 23, 2003Date of Patent: May 11, 2010Assignee: DSM IP Assets B.V.Inventors: Wilhelmus Hubertus Joseph Boesten, Joannes Gerardus Theodorus Kierkels, Friso Bernard Jan Assema, Luis Miguel Ruiz Pérez, Dolores González Pacanowska, Jesús González López, Santiago De La Escalera Hueso
-
Publication number: 20100112660Abstract: The present invention provides an effective method for derivatization of proteins using hydrostatic pressure to reversibly perturb the native conformation of a protein such that a normally buried functional group on the protein, such as an amino acid residue, or a ligand or cofactor associated with the protein, is exposed and available for derivatization by a polymer molecule or a cytotoxic agent. The methods described herein do not require use of chaotropes, changes in pH, changes in temperature, or genetic modification of the native primary sequence of the protein and are applicable to substantially all proteins.Type: ApplicationFiled: May 29, 2009Publication date: May 6, 2010Applicant: BaroFold, Inc.Inventor: Mary S. Rosendahl
-
Patent number: 7709235Abstract: Disclosed are a novel hydantoin racemase and a process for producing an optically active N-carbamylamino acid or an optically active amino acid using the hydantoin racemase. A novel hydantoin racemase isolated and purified from Bacillus sp. Strain KNK519HR; a gene encoding the hydantoin racemase; a recombinant plasmid having the gene introduced therein; a transformant having the hydantoin racemase gene introduced therein; and a process for producing an optically active N-carbamylamino acid or an optically active amino acid characterized in that a 5-substituted hydantoin compound is treated in the presence of hydantoinase and N-carbamylamino acid amidohydrolase as well as the hydantoin racemase.Type: GrantFiled: January 26, 2006Date of Patent: May 4, 2010Assignee: Kaneka CorporationInventors: Kenichi Nishi, Satohiro Yanagisawa, Hirokazu Nanba, Makoto Ueda, Naoto Noro
-
Publication number: 20100105885Abstract: Providing 1- or 6-deoxy products corresponding to all of aldohexoses, ketohexoses and sugar alcohols, as based on Deoxy-Izumoring, as well as a method for systematically producing those products. A method for producing deoxyketohexose and a derivative thereof using a deoxyketohexose isomerase derived from Pseudomonas cichorii ST-24 (FERM BP-2736), comprising epimerizing 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose at position 3 to produce the individually corresponding 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose as an intended product.Type: ApplicationFiled: November 20, 2007Publication date: April 29, 2010Applicants: NAT'L UNIVERSITY CORPORATION KAGAWA UNIVERSITY, RARE SUGAR PRODUCTION TECHNICAL RESEARCH LABS., MATSUTANI CHEMICAL INDUSTRY CO., LTD., KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKYUJOInventors: Ken Izumori, Masaaki Tokuda, George Fleet, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
-
Publication number: 20100086532Abstract: The present invention is directed to chimeric recombinases comprising a serine recombinase operatively linked to a zinc finger nucleotide binding domain such that the chimeric recombinase protein catalyzes site-specific recombination at a DNA site specifically bound by the zinc finger nucleotide binding domain. The serine recombinase can be one of several naturally occurring serine recombinases. The invention also includes nucleic acids encoding the chimeric recombinases, vectors including the nucleic acids, host cells transformed or transfected with the vectors, methods of using the chimeric recombinases to carry out recombination, methods of using substrate-linked protein evolution to generate additional chimeric recombinases, methods of using the chimeric recombinases for gene therapy, and pharmaceutical compositions.Type: ApplicationFiled: July 5, 2007Publication date: April 8, 2010Applicant: The Scripps Research InstituteInventors: Carlos F. Barbas, III, Russell M. Gordley
-
Patent number: 7691619Abstract: The invention relates to an isolated protein including an amino acid sequence represented by SEQ ID NO:2 and having an L-rhamnose isomerase activity. This novel enzyme has a higher reaction efficiency between D-psicose and D-allose and is excellent in thermal stability.Type: GrantFiled: August 23, 2005Date of Patent: April 6, 2010Assignee: Rare Sugar Production Technical Research Laboratories, LLCInventors: Ken Izumori, Goro Takata, Masaaki Tokuda
-
Publication number: 20100068766Abstract: It is an object of the present invention to provide a composition for catalyzing the cleavage of a single-stranded DNA and the binding of such single-stranded DNA. The present invention provides a composition for cleaving a single-stranded DNA and/or binding the 5?-terminus of such single-stranded DNA to the 3?-terminus thereof, which comprises an Ev1 protein. Moreover, the present invention also provides a composition for cleaving a single-stranded DNA and/or binding the 5?-terminus of such single-stranded DNA to the 3?-terminus thereof, which further comprises a Rad51B protein and/or a DNA topoisomerase type I protein, as well as the Ev1 protein.Type: ApplicationFiled: March 7, 2008Publication date: March 18, 2010Applicant: WASEDA UNIVERSITYInventors: Hitoshi Kurumizaka, Motoki Takaku, Shinichi Machida
-
Publication number: 20100047218Abstract: A gene complex for reversibly immortalizing cells contains an immortalizing gene region, which possesses at least a resistance gene, an immortalizing gene and, preferably, a suicide gene, and also two sequences which flank the gene region and which function as recognition sites for homologous intramolecular recombination, and at least one promoter located upstream of the gene region. A gene complex for immunomodulating cells contains a first immunomodulating gene region, whose expression inhibits the function of MHC I molecules, a second immunomodulating gene region, whose expression leads to the inactivation of natural killer cells, and a resistance gene. A method for obtaining cells involves preparing organ-related cells which are immortalized by transferring the first gene complex and immunomodulated by transferring the second gene complex. After the immortalized cells have been expanded, the immortalization is reversed.Type: ApplicationFiled: November 3, 2009Publication date: February 25, 2010Applicant: HEART BIOSYSTEMS GMBHInventors: JAN-HEINER KUPPER, REINHARD KANDOLF, ANNE KUHN
-
Patent number: 7666653Abstract: The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.Type: GrantFiled: June 7, 2007Date of Patent: February 23, 2010Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Hajime Ikeda, Yoshiyuki Yonetani, Shin-ichi Hashimoto, Makoto Yagasaki, Kenji Soda
-
Patent number: 7655451Abstract: Alanine 2,3-aminomutase sequences are disclosed, as are cells having alanine 2,3-aminomutase activity and methods of selecting for such cells. Methods for producing beta-alanine, pantothenate, 3-hydroxypropionic acid, as well as other organic compounds, are disclosed.Type: GrantFiled: November 9, 2007Date of Patent: February 2, 2010Assignee: Novozymes A/SInventors: Hans H. Liao, Ravi R. Gokarn, Steven J. Gort, Holly J. Jessen, Olga Selifonova
-
Publication number: 20090325231Abstract: Object: To provide a thermostable L-ribose isomerase. Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose.Type: ApplicationFiled: March 26, 2007Publication date: December 31, 2009Applicants: NAT'L UNIVERSITY CORPORATION KAGAWA UNIVERSITY, RARE SUGAR PRODUCTION TECHNICAL RESEARCH LABS., MATSUTANI CHEMICAL INDUSTRY CO., LTD., KABUSHIKI KAISHA HAYASHIBARA SEIBUTSU KAGAKU KENKUJOInventors: Ken Izumori, Kenji Morimoto, Goro Takata, Masaaki Tokuda, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
-
Publication number: 20090305351Abstract: The present invention relates to a method for producing a recombinant protein capable of increasing expression rate of a target protein and also improving solubility and folding of the expressed target protein using a modified protein disulfide isomerase (PDI) as a fusion partner, and an expression vector containing the modified PDI gene as a fusion partner. The method for preparing a recombinant protein using a modified PDI as a fusion partner according to the present invention may solve the problems concerning a low yield and solubility and folding that conventional fusion partners have, and be widely used for protein drug and industrial protein production.Type: ApplicationFiled: October 24, 2005Publication date: December 10, 2009Inventors: Hang-Cheol Shin, Yean-Hee Park, Hyang-Do Song, Eung-Yoon Kim, Ha-A-Rin Chon, Hye-Ran Hyun
-
Patent number: 7622284Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolised by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: GrantFiled: January 23, 2003Date of Patent: November 24, 2009Assignee: Royal Nedalco B.V.Inventors: Hubertus Johannes Marie Op Den Camp, Harry Ramanoedj Harhangi, Christiaan Van Der Drift, Jacobus Thomas Pronk
-
Publication number: 20090270506Abstract: The present invention relates to crystal forms of mammalian OSC and the crystal structure information obtained from them, to methods of preparing such crystal forms, and to their use for the identification and/or design of inhibitors of OSC activity. A further subject matter of the invention are methods for the identification and/or design of inhibitor compounds of OSC activity, the inhibitor compounds of OSC activity identified by these methods and their use in pharmaceutical compositions for the treatment and/or prevention of diseases which are associated with OSC comprising hypercholesterolemia, hyperlipemia, arteriosclerosis, vascular diseases, mycoses, parasite infections and gallstones, and/or treatment and/or prophylaxis of impaired glucose tolerance, diabetes, tumors and/or hyperproliferative disorders, preferably for the treatment and/or prophylaxis of hypercholesterolemia and/or hyperlipemia.Type: ApplicationFiled: March 3, 2008Publication date: October 29, 2009Inventors: ARMIN RUF, TANJA SCHULZ-GASCH, RALF THOMA
-
Publication number: 20090269827Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering metabolism in a cell using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.Type: ApplicationFiled: February 26, 2009Publication date: October 29, 2009Applicant: Battelle Energy Alliance, LLC.Inventors: Vicki S. Thompson, William A. Apel, David W. Reed, Brady D. Lee, David N. Thompson, Francisco F. Roberto, Jeffrey A. Lacey
-
Patent number: 7608416Abstract: The present invention relates to methods for the treatment of inflammation and pain without increasing cardiovascular risk by administration of an inhibitor of mPGES-1. The invention further is related to identifying inhibitors of mPGES-1 that do not increase cardiovascular risk when administered to an individual.Type: GrantFiled: December 7, 2005Date of Patent: October 27, 2009Assignee: The Trustees of the University of PennsylvaniaInventor: Garret A. Fitzgerald
-
Publication number: 20090226882Abstract: The invention relates to a method for identifying fungicides, to the use of fungal IPP isomerase for identifying fungicides, and to the use of inhibitors of the IPP isomerase as fungicides.Type: ApplicationFiled: May 19, 2006Publication date: September 10, 2009Applicant: Bayer Cropscience AGInventors: Peter Schreier, Birgitta Leuthner, Thorsten Leicher
-
Publication number: 20090226999Abstract: The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.Type: ApplicationFiled: June 7, 2007Publication date: September 10, 2009Inventors: Hajime Ikeda, Yoshiyuki Yonetani, Shin-ichi Hashimoto, Makoto Yagasaki, Kenji Soda
-
Patent number: 7585656Abstract: The present invention provides the crystal structure of the Trypanosoma cruzi PRACA proline racemase. Methods of modelling drugs that treat or prevent infection by T. cruzi are also provided, as are the drugs that are identified.Type: GrantFiled: May 26, 2004Date of Patent: September 8, 2009Assignees: Institute Pasteur, Centre National de la Recherche Scientifique (CNRS)Inventors: Paola Minoprio, Pedro Alzari, Alejandro Buschiazzo, Christophe Grégoire, Armand Berneman, Wim M. Degrave
-
Patent number: 7582454Abstract: The present invention relates to a 5-substituted hydantoin racemase, which efficiently catalyzes racemization reactions at a high optimum temperature for racemization reactions, DNA coding for the racemase, and processes for producing optically active amino acids.Type: GrantFiled: July 12, 2006Date of Patent: September 1, 2009Assignee: Ajinomoto Co. Inc.Inventors: Shunichi Suzuki, Norimasa Onishi, Kenzo Yokozeki
-
Publication number: 20090163376Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.Type: ApplicationFiled: December 18, 2008Publication date: June 25, 2009Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe