Aliphatic Patents (Class 435/249)
  • Patent number: 9738851
    Abstract: The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.
    Type: Grant
    Filed: April 3, 2014
    Date of Patent: August 22, 2017
    Assignee: DSM IP Assets B.V.
    Inventors: Craig M. Ruecker, Swithwin Patrick Adu-peasah, Brian S. Engelhardt, George T Veeder, III
  • Patent number: 8852918
    Abstract: A biologically pure isolate of a selected bacterium derived from Clostridium autoethanogenum is described which has improved efficiency in the production of ethanol by anaerobic fermentation of substrates comprising carbon monoxide. The bacterium can produce ethanol and acetate at an ethanol to acetate ratio of at least 1.0 and has a productivity of at least 1.2 g of ethanol/l of fermentation broth per day. The bacterium is also characterized in that it has substantially no ability to sporulate.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: October 7, 2014
    Assignee: Lanzatech New Zealand Limited
    Inventors: Sean Dennis Simpson, Richard Llewellyn Sydney Forster, Phuong Loan Tran, Matthew James Rowe, Ian Linstrand Warner
  • Patent number: 8211687
    Abstract: A thixotropic gel suitable for use in subsurface bioremediation is provided along with a process of using the gel. The thixotropic gel provides a non-migrating injectable substrate that can provide below ground barrier properties. In addition, the gel components provide for a favorable environment in which certain contaminants are preferentially sequestered in the gel and subsequently remediated by either indigenous or introduced microorganisms.
    Type: Grant
    Filed: June 17, 2010
    Date of Patent: July 3, 2012
    Assignee: Savannah River Nuclear Solutions, LLC
    Inventor: Brian D. Riha
  • Patent number: 8003355
    Abstract: A method of separating a polymer from a biomass containing the polymer, includes contacting the biomass with a solvent system, the solvent system including a solvent for the polymer and a precipitant for the polymer, to provide a residual biomass and a solution that includes the polymer, the solvent for the polymer and the precipitant for the polymer; and applying a centrifugal force to the solution and residual biomass to separate at least some of the solution from the residual biomass; where, the polymer is a polyhydroxyalkanoate; the precipitant comprises at least one alkane; the solvent for the polymer is selected from the group consisting of ketones, esters and alcohols and combinations thereof; and the biomass comprises a slurry of the biomass and water.
    Type: Grant
    Filed: April 15, 2010
    Date of Patent: August 23, 2011
    Assignee: Metabolix, Inc.
    Inventors: Johan van Walsem, Luhua Zhong, Simon S. Shih
  • Publication number: 20030235895
    Abstract: A microbial culture and method for producing wax esters using highly branched alkanes. In accordance with one embodiment, the highly branched alkane is squalane.
    Type: Application
    Filed: January 29, 2003
    Publication date: December 25, 2003
    Inventors: William W. Bogan, Wendy R. Sullivan, James R. Paterek
  • Patent number: 6569909
    Abstract: The present invention relates to methods of inhibiting growth and reproduction of microorganisms in rapidly biodegradable hydrocarbonaceous products, containing minor amounts of aqueous liquids. The present invention also relates to rapidly biodegradable hydrocarbonaceous products containing an effective amount of a petroleum-derived hydrocarbonaceous product such that the rapidly biodegradable hydrocarbonaceous product resists visible growth of microorganisms.
    Type: Grant
    Filed: October 18, 2001
    Date of Patent: May 27, 2003
    Assignee: Chervon U.S.A., Inc.
    Inventors: Kirk T. O'Reilly, Michael E. Moir, Dennis J. O'Rear
  • Patent number: 6410265
    Abstract: This invention relates to the isolation of a novel putative efflux gene from Pseudomonas mendocina. The putative efflux gene is useful for probing an organism's efflux system to gain an understanding of the mechanisms of solvent tolerance. The invention further provides a Pseudomonas mendocina strain deficient in this gene. This strain is unable to grow in the presence of chloramphenicol and, compared to the wildtype strain, grows slowly in the presence of high concentrations of PHBA.
    Type: Grant
    Filed: October 30, 1998
    Date of Patent: June 25, 2002
    Assignee: E. I. du Pont de Nemours and Company
    Inventor: Kevin Keqin Chen
  • Patent number: 6379937
    Abstract: The present invention relates to a novel process for the preparation of mixture of 19 hydroxyeicosatetraenoic acid and 20 hydroxyeicosatetraenoic acid (19 HETE and 20 HETE) by biotransformation of Arachidonic acid or Poly Unsaturated Fatty Acids (PUFA) with yeast as the biotransforming agent.
    Type: Grant
    Filed: December 18, 2000
    Date of Patent: April 30, 2002
    Assignee: Council of Scientific and Industrial Research
    Inventors: Asmita Ashutosh Prabhune, Colin Ratledge
  • Patent number: 5900366
    Abstract: A process for the fed batch production of a sophorolipid composition is described, in which culturing takes place of at least one Candida bombicola strain in a culture medium incorporating a sugar and a nitrogen source and said cultured strain is exposed in a reaction zone to a supply of an appropriate substrate under adequate aeration, temperature and pH conditions and at least once the following sequence is performed: a) the strain is continuously supplied with substrate at a supply rate in the reaction zone between 0.
    Type: Grant
    Filed: June 18, 1993
    Date of Patent: May 4, 1999
    Assignee: Institut Fran.cedilla.ais Du Petrole
    Inventors: Remy Marchal, Jeannine Lemal, Caroline Sulzer, Anne-Marie Davila
  • Patent number: 5807724
    Abstract: Organisms that degrade hydrocarbon-based substances are encapsulated in wax to form organism-containing wax microshells that are used for removing oil-based substances such as oil spills on land and water. The organism is preferably of the genus Candida that produce lipase. The microshell may contain nutrients that sustain the organism. The wax is paraffin wax or beeswax, and the microshell may be coated with talc or carbon powder to provide insulation and to prevent one microshell from sticking to another. When used for remediating an oil spill on water, the microshells are preferably contained in a buoyant container that acts as a flotation device. The flotation device may contain flotation means such as gas-filled microballoons that facilitate flotation.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: September 15, 1998
    Inventor: Joseph A. Resnick
  • Patent number: 5756317
    Abstract: Heteropolysaccharide biopolymers well adopted as thickening agents are improvedly produced by microbially fermenting a carbohydrate nutrient medium, said nutrient medium comprising an oil-in-water emulsion of a discontinuous oily phase dispersed within a continuous aqueous phase.
    Type: Grant
    Filed: January 16, 1991
    Date of Patent: May 26, 1998
    Assignee: Rhone-Poulenc Specialities Chimiques
    Inventors: Veronique Leproux, Michel Peignier, Patrick Cros, Jeanine Beucherie, Yves Kennel
  • Patent number: 5707825
    Abstract: This invention provides an interface bioreactor system comprising (a) a reaction tank having (1) a reaction solvent placed therein, (2) a plurality of plate-like hydrophilic immobilizing carriers each having a thickness of 1 to 500 mm and packed therein so as to be arranged in a vertical position at intervals of 3 to 100 mm, the hydrophilic immobilizing carriers having a microorganism deposited and immobilized on the surfaces thereof in a growable or viable state and containing a liquid medium composed of water and nutrients necessary for the growth or survival of the microorganism, and (3) a sparger or agitator disposed at the bottom thereof; and (b) an aeration line connected with the reaction tank for supplying oxygen thereto. This system is highly efficient and can be scaled up easily.
    Type: Grant
    Filed: July 26, 1996
    Date of Patent: January 13, 1998
    Assignee: Kansai Paint Co., Ltd.
    Inventor: Shinobu Oda
  • Patent number: 5370990
    Abstract: A method for the determination of glycated protein in a sample characterised in that it comprises treating the sample with a protease and treating the protease-treated sample with a ketoamine oxidase, a product of this reaction being measured is disclosed.
    Type: Grant
    Filed: July 27, 1992
    Date of Patent: December 6, 1994
    Assignee: Genzyme Corporation
    Inventors: Julie M. Staniford, John A. Power, John A. Lovelady
  • Patent number: 5364789
    Abstract: A microbial cleaner comprising at least one hydrocarbon-ingesting microbe strain and a biocatalyst transforms hydrocarbons into nontoxic substances. The biocatalyst includes a nonionic surfactant, a chlorine-absorbing salt, at least one microbe nutrient, and water. The cleaner can be used in virtually any situation requiring the removal of hydrocarbon, including cleaning contaminated soil and treating oil spills on soil and water.
    Type: Grant
    Filed: January 14, 1994
    Date of Patent: November 15, 1994
    Inventors: Lloyd J. Guinn, James L. Smith
  • Patent number: 5290688
    Abstract: Method for the culture of Babesia divergens, characterized in that the Babesia strain is maintained under culture in a culture medium free from serous protein but containing lipoproteins and red blood corpuscles, and a method for preparing exoantigens and a vaccine containing these antigens.
    Type: Grant
    Filed: October 17, 1991
    Date of Patent: March 1, 1994
    Assignee: Rhone Merieux
    Inventors: Joseph Schrevel, Andre Gorenflot, Eric Precigout, Alain Marchand, Philippe Brasseur, Monique L'Hostis, Daniel Rigomier, Alexis Valentin, Emmanuel Vidor, Guy Bissuel
  • Patent number: 5262306
    Abstract: Methods for the isolation and purification of the phytotoxin cercosporin are disclosed as well as methods for identifying microorganisms capable of degrading cercosporin. Cercosporin can be purified from members of the fungal genus Cercospora and incorporated into culture medium for selection of those organisms resistant to cercosporin. Once identified, these organisms can be used to isolate the protein and the gene responsible for conferring cercosporin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for cercosporin-resistance can be inserted into a vector suitable for transforming an Agrobacterium and the Agrobacterium in turn used to transform plant cells normally susceptible to Cercospora infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading cercosporin.
    Type: Grant
    Filed: April 3, 1990
    Date of Patent: November 16, 1993
    Inventors: David J. Robeson, Mahbubul A. F. Jalal, Robert B. Simpson
  • Patent number: 5192672
    Abstract: A purified hydroxylase component of the soluble methane monooxygenase enzyme present in the bacterium Methylosinus trichosporium OB3b is found capable of oxidizing hydrocarbons under aerobic conditions in the presence of suitable reducing agents. The hydroxylase can be reduced by commercial reducing agents, such as sodium dithionite and photo- and electrochemical means when in the presence of electron transport components, such as methyl viologen and proflavin. The hydroxylase can also be activated by hydrogen peroxide in the absence of reducing agents and molecular oxygen and is capable of oxidizing hydrocarbons under aerobic and anaerobic conditions in this manner. The hydroxylase component can be obtained with high final specific activity when ferrous iron compounds and cysteine are included in the purification buffers used to extract the hydroxylase from bacterial cells.
    Type: Grant
    Filed: June 21, 1991
    Date of Patent: March 9, 1993
    Assignee: Amoco Corporation
    Inventor: John D. Lipscomb
  • Patent number: 5190870
    Abstract: A purified hydroxylase component of the soluble methane monooxygenase enzyme present in the bacterium Methylosinus trichosporium OB3b is found capable of oxidizing hydrocarbons under aerobic conditions in the presence of suitable reducing agents. The hydroxylase can be reduced by commercial reducing agents, such as sodium dithionite and photo- and electrochemical means when in the presence of electron transport components, such as methyl viologen and proflavin. The hydroxylase component can be obtained with high final specific activity when ferrous iron compounds and cysteine are included in the purification buffers used to extract the hydroxylase from bacterial cells.
    Type: Grant
    Filed: October 8, 1990
    Date of Patent: March 2, 1993
    Assignee: Amoco Corporation
    Inventors: John D. Lipscomb, Brian G. Fox
  • Patent number: 5128262
    Abstract: The microbial decontamination of soils contaminated with hydrocarbons, in particular with mineral oils, by microbial oxidation is improved by a process in which in addition to supplying the oxygen necessary for degradation, by aeration, soluble nutrients, water and soluble or dispersed biosurfactants as such or mixed with chemical surfactants are metered into the contaminated soils directly or into the removed contaminated soil layers, in one or more batches at time intervals, in an amount such that the formation of toxic intermediates which slow down or block microbial degradation in prevented, the accessibility of mineral oils and their products for the microorganisms is simultaneously increased and, as a result, accelerated, virtually complete mineralization by respiration to CO.sub.2 and H.sub.2 O is effected.
    Type: Grant
    Filed: September 30, 1988
    Date of Patent: July 7, 1992
    Assignee: Wintershell AG
    Inventors: Walter Lindoerfer, Kai-Udo Sewe, Axel Oberbremer, Reinhard Mueller-Hurtig, Fritz Wagner
  • Patent number: 5128250
    Abstract: A process for the production of a highly unsaturated fatty acid having an odd number of carbon atoms by culturing a microorganism belong to the genus Mortierella and capable of producing the fatty acid; and a process for the production a highly unsaturated fatty acid having an odd number of carbon atoms, typically represented by 8,11,14-nonadecatrienoic acid, by culturing a microorganism capable of producing arachidonic acid.
    Type: Grant
    Filed: March 9, 1989
    Date of Patent: July 7, 1992
    Assignee: Suntory Limited
    Inventors: Kengo Akimoto, Yoshifumi Shinmen, Hideaki Yamada, Sakayu Shimizu
  • Patent number: 5059532
    Abstract: Disclosed herein is a process for producing an unsaturated fatty acid or derivative thereof which comprises culturing an unsaturated fatty acid-producing microorganism belonging to the genus Rhodococcus in a medium containing a saturated fatty acid or a derivative thereof, or causing the resting cells of the microorganisms to act on a saturated fatty acid or a derivative thereof. Also disclosed herein is a process for producing an alkene or derivative thereof which comprises culturing an unsaturated hydrocarbon compound-producing microorganism belonging to the genus Rhodococcus in a medium containing an alkane or derivative thereof, or causing the resting cells of the microorganism to act on an alkane or derivative thereof.
    Type: Grant
    Filed: December 1, 1987
    Date of Patent: October 22, 1991
    Assignee: Agency of Industrial Science & Technology
    Inventors: Yoshiharu Kimura, Shigehito Adachi, Katsutoshi Ara, Shigeo Inoue, Kenzo Koike
  • Patent number: 4981800
    Abstract: A method for culturing microorganisms belonging to the genus Pseudomonas or the genus Escherichia and having tolerance to an organic solvent such as any one of hydrocarbons, alcohols, ethers, ketones and their derivatives or their mixture in a medium containing the organic solvent in a concentration of 0.3% or more. The present method can be widely utilized in the fields of bioreactor, liquid-waste treatment, protein engineering, etc.
    Type: Grant
    Filed: March 29, 1988
    Date of Patent: January 1, 1991
    Assignee: Research Development Corporation of Japan
    Inventors: Akira Inoue, Kouki Horikoshi
  • Patent number: 4910143
    Abstract: A mixture of Pseudomonas putida one having plasmids encoding the camphor (CAM) and toluene (TOL) degradation and the other having a plasmid encoding for naphthalene (NAH) degradation is described. The mixture is more effective than either Pseudomonas putida alone or than a single Pseudomonas putida with three related plasmids which encode for the degradation of CAM, TOL and NAH.
    Type: Grant
    Filed: December 8, 1986
    Date of Patent: March 20, 1990
    Assignee: Microlife Technics, Inc.
    Inventor: Peter A. Vandenbergh
  • Patent number: 4814272
    Abstract: Rhamnolipids with high surface activity are produced micro iologically in high yield per g of dry cell substance using Pseudomonas spec. DSM 2874 in the form of growing, resting and immobilized cell mass in an aqueous medium containing at least one assimilable carbon source at a pH of 6.7 to 7.3 and a temperature of 30.degree. to 37.degree. C. Two new rhamnolipids with only one .beta.-hydroxydecanoic acid residue in the molecule and defined as .alpha.-L-rhamnopyranosyl- and 2-O-.alpha.-L-rhamnopyranosyl-.alpha.-L-rhamnopyranosyl-.beta.-hydroxydeca noic acid with a molecular weight of 334 and 480, respectively, are obtained.
    Type: Grant
    Filed: February 15, 1985
    Date of Patent: March 21, 1989
    Assignee: Wintershall AG
    Inventors: Fritz Wagner, Christoph Syldatk, Uwe Matulowic, Hans-Jurgen Hofmann, Kai-Udo Sewe, Walter Lindorfer
  • Patent number: 4806482
    Abstract: The subject invention concerns a novel plasmid and its use in a microbial host to degrade a variety of organic compounds. Some of these compounds, such as ethylene dichloride, are undesirable waste products found in various dump sites. The invention also concerns a novel microbe hosting the novel plasmid. The novel plasmid has been shown to encode the gene(s) responsible for the degradation of the organic compounds. Thus, microbes hosting this plasmid, denoted pEDC, can be used to degrade ethylene dichloride, and other compounds.
    Type: Grant
    Filed: June 6, 1988
    Date of Patent: February 21, 1989
    Assignee: The B.F. Goodrich Company
    Inventor: Amikam Horowitz
  • Patent number: 4798723
    Abstract: Agricultural methods of biological control and organisms useful in such methods are disclosed, such as a plant colonizing organisms and methods of biologically controlling plant disease caused by fungi. A novel type of Pseudomonas cepacia type Wisconsin has been identified. These strains and other P. cepacia strains described are useful as vectors for delivery to plants of beneficial products.
    Type: Grant
    Filed: October 5, 1987
    Date of Patent: January 17, 1989
    Assignee: Lubrizol Genetics, Inc.
    Inventors: Peter J. Dart, K. Prakash Hebber
  • Patent number: 4720456
    Abstract: Anionic, surface-active trehalose lipids, in which different organic acids are bound to a trehalose molecule by an ester linkage, are prepared by aerobically cultivating trehalose-producing microorganisms, capable of assimilating hydrocarbons, under growth-limiting conditions, but without limiting the oxygen.
    Type: Grant
    Filed: August 20, 1986
    Date of Patent: January 19, 1988
    Assignee: Wintershall AG
    Inventors: Fritz Wagner, Egbert Ristau, Zu-yi Li, Siegmund Lang, Walther Schulz, Hans-Jurgen Hofmann, Kai-Udo Sewe, Walter Lindorfer
  • Patent number: 4657863
    Abstract: This invention provides a process for stabilizing a population of a mutant microorganism in a bioconversion system whereby growth of revertant cells is suppressed.The process involves limiting a nutrient which is essential for cell growth so that the cells selectively grow on a growth carbon source rather than on a non-growth carbon source which is present. The revertant cells being suppressed have a similar ability as the parent strain of the mutant microorganism to grow on the non-growth carbon source in the bioconversion system.The non-growth carbon source in the bioconversion system is metabolized to an extracellular accumulating quantity of a desired metabolite, e.g., toluene is converted to muconic acid.
    Type: Grant
    Filed: April 11, 1983
    Date of Patent: April 14, 1987
    Assignee: Celanese Corporation
    Inventors: Peter C. Maxwell, Jin-Han Hsieh, John C. Fieschko
  • Patent number: 4618582
    Abstract: In the aerobic culture of microorganisms of the Xanthomonas genus in fermentation media, the use of water-in-oil emulsions in the media minimizes viscosity problems and enhances yields of Xanthomonas biopolymers. Preferably, the emulsion is formed with a surfactant and the microorganism is Xanthomonas campestris.
    Type: Grant
    Filed: February 8, 1982
    Date of Patent: October 21, 1986
    Assignee: Henkel Kommanditgesellschaft auf Aktien
    Inventors: Konrad Engelskirchen, Werner Stein, Michael Bahn, Ludwig Schieferstein, Joachim Schindler, Rolf Schmid
  • Patent number: 4617274
    Abstract: Novel yeasts are disclosed including Pichia pastoris NRRL Y-11430, yeasts having the characteristics of Pichia pastoris NRRL Y-11430, mutants of Pichia pastoris NRRL Y-11430, and strains derived therefrom. Also disclosed are methods of culturing the strains, and biochemical conversions employing the strains.
    Type: Grant
    Filed: September 22, 1983
    Date of Patent: October 14, 1986
    Assignee: Phillips Petroleum Company
    Inventor: Eugene H. Wegner
  • Patent number: 4588584
    Abstract: A new biotype, SDL-POP-S-1, of the soilborne beneficial bacterium Pseudomonas cepacia NRRL B-14149 has been discovered. The biotype is very effective in controlling Pythium diseases of cucumbers and peas. A new medium that is exclusively selective for the bacterium Pseudomonas cepacia has also been developed.
    Type: Grant
    Filed: June 1, 1983
    Date of Patent: May 13, 1986
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventors: Robert D. Lumsden, Myron Sasser
  • Patent number: 4535059
    Abstract: This invention provides an improved fermentation process for bioconversion of toluene to muconic acid.The process involves operating the bioconversion system under phosphate-limiting conditions so as to achieve an increase in specific muconic acid productivity with a stabilized population of microorganism such as an ATCC No. 31,916 type of Pseudomonas putida Biotype A mutant strain.
    Type: Grant
    Filed: January 13, 1983
    Date of Patent: August 13, 1985
    Assignee: Celanese Corporation
    Inventors: Jih-Han Hsieh, Sol J. Barer, Peter C. Maxwell
  • Patent number: 4480034
    Abstract: This invention provides a continuous bioconversion process in which a cross-flow membrane filtration zone is employed to recover a whole cell-containing retentate stream and a cell-free bioconversion product-containing permeate stream. The retentate stream is recycled to the fermentation zone. In a specific embodiment, toluene is bio-oxidized to muconic acid with a microorganism such as Pseudomonas putida Biotype A strain ATCC 31,916. The muconic acid is recovered as a precipitate from the cell-free permeate fermentation broth, and the fermentation broth is recycled in the process.
    Type: Grant
    Filed: June 10, 1982
    Date of Patent: October 30, 1984
    Assignee: Celanese Corporation
    Inventor: Jih-Han Hsieh
  • Patent number: 4473641
    Abstract: Protein is produced by bacterial degradation of rubber in a nutrient-containing, preferably sterile environment. The resultant protein is particularly useful as an animal feed.
    Type: Grant
    Filed: August 22, 1979
    Date of Patent: September 25, 1984
    Inventor: Henry E. Voegeli
  • Patent number: 4424274
    Abstract: The present invention relates to a microbiological process for the production of citric acid by assimilation of .alpha.-olefins, normal paraffins and their mixture. This process is carried out by culturing the microorganisms selected from the group of Candida tropicalis, Candida lipolytica, Candida intermedia and Canida brumptii and their mutants and their variants under higher concentration of dissolved oxygen than that in ordinary aeration in the culture medium. The concentration of dissolved oxygen in the culture medium is suitable in the range of from 5 to 40 ppm, preferably from 10 to 30 ppm on the weight basis of said culture medium in this invention.There is many means of which increase the dissolved oxygen in the culture medium.
    Type: Grant
    Filed: May 6, 1982
    Date of Patent: January 3, 1984
    Assignee: Showa Oil Company, Ltd.
    Inventors: Takao Matsumoto, Yoshiyuki Ichikawa, Takeo Nagata
  • Patent number: 4414329
    Abstract: A method of high cellular density yeast fermentation at high mineral salts feed to and maintained in the ferment.Single cell protein (SCP) is produced in an aerobic fermentation process at high yields under high cell density conditions employing media of high mineral salts concentration. Novel Pichia pastoris and Hansenula polymorpha yeasts are disclosed.
    Type: Grant
    Filed: October 29, 1981
    Date of Patent: November 8, 1983
    Assignee: Phillips Petroleum Company
    Inventor: Eugene H. Wegner
  • Patent number: 4411998
    Abstract: The present invention relates to a suppressing method of iso-citric acid formation in producing citric acid from hydrocarbons by fermentation.This process is carried out by culturing the microorganisms selected from the group belonging to Candida tropicalis, Candida lipolytica, Candida intermedia and Candida brumptii and their mutants and variants in the culture medium containing paraffinic and olefinic hydrocarbons and their mixture as carbon source under aerobic conditions, wherein specific non-ionic surface active agent is added to said culture medium.The specific non-ionic surface active agent added to said culture medium is selected from the group of sorbitan fatty acid esters and polyoxy-ethylene sorbitan fatty acid esters. The amount of specific surface active agent added to said culture medium is enough from 0.005 to 0.5 percent by weight, preferably from 0.02 to 0.2 percent on the weight basis of said culture medium.
    Type: Grant
    Filed: March 19, 1982
    Date of Patent: October 25, 1983
    Assignee: Showa Oil Company, Ltd.
    Inventors: Takao Matsumoto, Atsushi Fujimaki, Takeo Nagata
  • Patent number: 4391908
    Abstract: Citric acids are produced by culturing a citric acids-accumulating and hydrocarbon-assimilating strain of a yeast of the genus Candida in an aqueous medium containing, as main carbon source, at least one normal paraffin with from 9 to 20 carbon atoms, inclusive, in the molecule, at a specific pH, and recovering accumulated citric acids from the culture broth.
    Type: Grant
    Filed: June 16, 1978
    Date of Patent: July 5, 1983
    Assignee: Takeda Chemical Industries, Ltd.
    Inventors: Takeshi Tabuchi, Matazo Abe
  • Patent number: 4389484
    Abstract: Citric acids are produced by culturing a citric acids-accumulating and hydrocarbon-assimilating strain of a yeast of the Genus Candida in an aqueous medium containing, as main carbon source, at least one normal paraffin with from 9 to 20 carbon atoms, inclusive, in the molecule, at a specific pH, and recovering accumulated citric acids from the culture broth.
    Type: Grant
    Filed: June 16, 1978
    Date of Patent: June 21, 1983
    Assignee: Takeda Chemical Industries, Ltd.
    Inventors: Takeshi Tabuchi, Matazo Abe
  • Patent number: 4385121
    Abstract: An improved landfarming medium and process is disclosed in which hydrocarbon wastes are disposed of by dispersing them in a medium composed of a mixture of soil containing hydrocarbon-oxidizing microorganisms and a spent, solid, particulate, porous hydrocarbon cracking catalyst and/or a spent, solid, particulate, porous filtration medium.
    Type: Grant
    Filed: October 7, 1981
    Date of Patent: May 24, 1983
    Assignee: Chevron Research Company
    Inventor: Harold E. Knowlton
  • Patent number: 4368267
    Abstract: A process is disclosed for the epoxidation of lower .alpha.-olefins dienes or vinyl aromatic compounds by contacting said compounds, under aerobic conditions in the presence of microorganisms, genetically engineered organisms thereof or enzyme preparations derived from said microorganisms. The microorganisms, genetically engineered organisms, or enzyme preparations derived therefrom are preferably those microorganisms which are known as methylotrophs particularly those which have previously grown under aerobic conditions in a nutrient medium containing methane.
    Type: Grant
    Filed: February 6, 1980
    Date of Patent: January 11, 1983
    Assignee: Exxon Research and Engineering Co.
    Inventors: Ching-Tsang Hou, Ramesh N. Patel, Allen I. Laskin
  • Patent number: 4355109
    Abstract: Newly isolated microbes of the genera Arthrobacter-Corynebacterium-Nocardia as represented by Corynebacteria Salvinicum strain SFC, produce substantial quantities of materials having outstanding surfactant properties, when grown on carbon supplying substrates under fermentation conditions.
    Type: Grant
    Filed: April 22, 1980
    Date of Patent: October 19, 1982
    Inventors: James E. Zajic, Donald F. Gerson, Richard K. Gerson, Chandrakant J. Panchal
  • Patent number: 4326035
    Abstract: Process for the culture of aerobic microorganisms in a culture medium provided with at least one source of oxygen, at least one source of carbon and at least one source of hydrogen, comprises providing oxygen originating from the decomposition of hydrogen peroxide as a source of oxygen and providing at least one highly inflammable product as a source of carbon and hydrogen.
    Type: Grant
    Filed: January 9, 1978
    Date of Patent: April 20, 1982
    Assignee: Interox
    Inventor: Rodolfo Gabellieri
  • Patent number: 4275158
    Abstract: A method for the oxidation of hydrocarbons to monocarboxylic acids and then to dicarboxylic acids by the aerobic cultivation or resting cell reaction of the organism Debaryomyces vanriji (BR-308) ATCC 20588.
    Type: Grant
    Filed: January 7, 1980
    Date of Patent: June 23, 1981
    Assignee: Bio Research Center Company, Ltd.
    Inventors: Akira Taoka, Seiichi Uchida
  • Patent number: 4273872
    Abstract: A method is disclosed for the microbiological transformation of steroids, especially cholesterol, by employing culture media which contain hydrocarbons rather than carbohydrates. Paraffinic hydrocarbons, particularly normal paraffins from 15 to 20 carbon atoms, are used as the only carbon and energy sources. SP2T (NRRL B-11, 112), SP3T bis (NRRL B-11, 113), SP5T (NRRL B-11, 114), SP7T/1 (NRRL B-11, 115), SP742XXA (NRRL B-11, 116), SP731RD (NRRL B-11, 117), and SP734D (NRRL B-11, 118) are bacterial strains which can be used to this purpose.
    Type: Grant
    Filed: November 13, 1979
    Date of Patent: June 16, 1981
    Assignee: Snamprogetti S.p.A.
    Inventors: Pasquale Zaffaroni, Vincenza Vitobello, Anna M. Gamalerio
  • Patent number: 4268630
    Abstract: A process is disclosed for the microbiological production of ketones from C.sub.3 -C.sub.6 alkanes by contacting C.sub.3 -C.sub.6 alkanes under aerobic conditions with resting microbial cells derived from a methylotrophic microorganism or enzyme preparation derived from said cells, wherein the microorganism has been previously grown under aerobic conditions in a nutrient medium containing methane or dimethyl ether.
    Type: Grant
    Filed: March 30, 1979
    Date of Patent: May 19, 1981
    Assignee: Exxon Research & Engineering Co.
    Inventors: Ramesh N. Patel, Ching-Tsang Hou, Allen I. Laskin
  • Patent number: 4172764
    Abstract: The cell walls are removed from two distinct auxotropic mutant strains of yeast. The protoplasts thus obtained are fused and the cell walls regenerated. The strains obtained are cultured on a medium where the original auxotrophs cannot develop and the hybrid or recombinant strains are recovered. The strains obtained by the process are useful for preparing yeast proteins.
    Type: Grant
    Filed: September 20, 1977
    Date of Patent: October 30, 1979
    Assignee: Agence Nationale de Valorisation de la Recherche (ANVAR)
    Inventors: Henri Heslot, Anne Provost, Philippe Fournier