Escherichia (e.g., E. Coli, Etc.) Or Salmonella Patents (Class 435/252.8)
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Patent number: 8003091Abstract: The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011 minicells.Type: GrantFiled: March 27, 2007Date of Patent: August 23, 2011Assignee: Engeneic Molecular Delivery Pty. Ltd.Inventors: Himanshu Brahmbhatt, Jennifer MacDiarmid
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Publication number: 20110195483Abstract: Compositions and methods of producing vaccines, including methods wherein whole organism vaccines are provided with limited replication abilities, thereby increasing vaccine safety and efficacy, through the use of non-natural, unnatural, or non-naturally encoded amino acids.Type: ApplicationFiled: September 28, 2009Publication date: August 11, 2011Applicant: AMBRX, INC.Inventors: Feng Tian, Brad Hehli
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Publication number: 20110177579Abstract: An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40±1 kDa. The gene encoding the enzyme was cloned and sequenced, and found to have significant sequence homology to known zinc-containing ADHs and L-threonine dehydrogenases with both binding motifs of catalytic zinc and NADP+. The wild-type enzyme is a primary-secondary ADH that exhibits a substrate preference for secondary alcohols and corresponding ketones, and exhibits unusual stereoselectivity. The wild-type enzyme was found to have outstanding thermostability, demonstrating 60% activity after incubation at 80° C. for 40 hours. Site-directed mutagenesis was used to substitute the cyteine residue at position 56 with a serine, to provide the TgADH(C56S) mutant. In the assays that we carried out, we found virtually no difference in enzyme activity and oxygen-sensitivity between the mutant TgADH (C56S) and wild type TgADH.Type: ApplicationFiled: March 25, 2011Publication date: July 21, 2011Inventors: Kesen MA, Xiangxian YING
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Patent number: 7981411Abstract: The present invention relates to the use of F4+ non-pathogenic Escherichia coli strains to promote growth in an animal. The present invention also relates to the use of such strains to homogenize growth among a herd of animals. More specifically, the animal(s) of interest in the present invention are those wherein growth promotion or growth homogenization are desired goals, such as animals reared for meat production. The present invention further relates to a method for promoting growth of an animal as well as a method for homogenizing growth among a herd of animals.Type: GrantFiled: February 3, 2005Date of Patent: July 19, 2011Assignee: Valorisation—Recherche, Limited PartnershipInventors: Eric Nadeau, John Morris Fairbrother
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Publication number: 20110171703Abstract: The present invention provides: a lactic acid-producing Escherichia coli including an enzymatic activity of at least one NAD-dependent lactate dehydrogenase and an enzymatic activity of at least one NAD-independent lactate oxidoreductase, both of which are enhanced so as to decompose one of D-lactic acid or L-lactic acid and to produce the other one of D-lactic acid or L-lactic acid; and a lactic acid production method using the lactic acid-producing Escherichia coli.Type: ApplicationFiled: September 11, 2009Publication date: July 14, 2011Inventors: Mitsufumi Wada, Katsuyuki Takahashi, Takashi Morishige, Daisuke Miyazawa, Hitoshi Takahashi, Daisuke Mochizuki, Tadashi Araki
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Patent number: 7972812Abstract: A novel cytokine designated TRAIL induces apoptosis of certain target cells, including cancer cells and virally infected cells. Isolated DNA sequences encoding TRAIL are disclosed, along with expression vectors and transformed host cells useful in producing TRAIL polypeptides. Antibodies that specifically bind TRAIL are provided as well.Type: GrantFiled: May 13, 2010Date of Patent: July 5, 2011Assignee: Immunex CorporationInventors: Steven R. Wiley, Raymond G. Goodwin
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Publication number: 20110159515Abstract: The invention relates to assay methods for use in detecting specific materials such as core oligosaccharides derived from microorganisms, particularly pathogenic microorganisms, in a test sample. The invention further relates to compositions and methods for the rapid growth of such microorganisms enabling detection of same significantly earlier than is currently possible. In particular embodiments the invention is directed towards the rapid growth and/or detection of Salmonella, Shigella or Listeria.Type: ApplicationFiled: September 10, 2009Publication date: June 30, 2011Applicant: SOLUS SCIENTIFIC SOLUTIONS LIMITEDInventor: William Stimson
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Patent number: 7968325Abstract: Coexpression of a polyhydroxyalkanoic acid synthase and a either a fatty acid:acyl-CoA transferase or an acyl-CoA synthetase in cells enables the biosynthesis of polyester materials. Plasmids, bacteria, materials, and methods for the preparation of polyesters are disclosed.Type: GrantFiled: June 14, 2004Date of Patent: June 28, 2011Assignee: Metabolix, Inc.Inventors: Silke Hein, Brigitte Sohling, Gerhard Gottschalk, Alexander Steinbuchel
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Publication number: 20110151511Abstract: The present invention provides novel rapidly growing microorganisms and methods for their use in cloning or subcloning nucleic acid molecules. The rapid growing microorganisms of the present invention form colonies more rapidly than microorganisms typically used in molecular biology and thus provide a significant improvement in in vitro cloning methods used extensively in molecular biology. The invention also relates to kits and compositions used in the methods of the invention.Type: ApplicationFiled: December 17, 2010Publication date: June 23, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Frederic R. Bloom, Brian J. Schmidt, Jhy-Jhu Lin
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Publication number: 20110150907Abstract: The present disclosure relates generally to recombinant bacteria (e.g., Lactobacillus) that express one or more binding peptides, antibodies and/or antibody binding fragments on their surface that are specific for one or more pathogens and/or toxins, including toxins from pathogens. The recombinant bacteria may be used for binding, removing and/or neutralizing one or more pathogens and/or toxins, including toxins from pathogens in a gastrointestinal tract.Type: ApplicationFiled: April 3, 2009Publication date: June 23, 2011Applicant: FARALLONE HOLDINGS BVInventors: Jos Seegers, Stephen F. Carroll
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Publication number: 20110142879Abstract: Methods of preparing a DNA comprising sequences derived from the genomic RNA (gRNA) of a coronavirus are provided, comprising cloning under the expression of a promoter a coronavirus interfering defective genome into a bacterial artificial chromosome (BAC) and reinserting into said genome the deleted sequences within the defective genome, wherein said sequences have a homology of at least 60% to the natural sequence of the virus, wherein said sequences code for an RNA-dependent RNA polymerase and at least one structural or non-structural protein, and wherein a fragment of said DNA is capable of being transcribed into RNA and assembeld to a virion. Also provided are infective clones, recombinant viral vectors, and vaccines.Type: ApplicationFiled: November 3, 2010Publication date: June 16, 2011Applicant: CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASInventor: LUIS ENJUANES SANCHEZ
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Patent number: 7960141Abstract: The invention relates to compositions and methods for the in vivo production of polypeptides comprising one or more unnatural amino acids. Specifically, the invention provides plasmid systems for the efficient eubacterial expression of polypeptides comprising one or more unnatural amino acids at genetically-programmed positions.Type: GrantFiled: March 7, 2007Date of Patent: June 14, 2011Assignee: The Scripps Research InstituteInventors: Youngha Ryu, Peter G. Schultz
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Publication number: 20110136211Abstract: Disclosed is an apparatus for culturing microorganisms and methods for using the same.Type: ApplicationFiled: December 8, 2009Publication date: June 9, 2011Inventor: Qi Zhong
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Patent number: 7947440Abstract: The present invention provides a method for measuring the specific rate of ribosome synthesis for a distinct cell population, such as a distinct microbial population. For an actively growing (or non-growing) culture, the specific rate of ribosome synthesis is identical to the specific growth rate of the culture. With the method of the invention, researchers will be able to measure the specific growth rate of distinct cell populations in mixed cultures, such as biological reactor systems or environmental samples. In addition, the method of the invention provides the ability to identify members of a distinct cell population that are rapidly growing.Type: GrantFiled: September 15, 2006Date of Patent: May 24, 2011Assignee: University of South FloridaInventors: Peter George Stroot, Matthew Raymond Cutter
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Publication number: 20110117132Abstract: The present invention relates to an attenuated Salmonella typhi having mutation in chromosomal gene loci, its use as a potent vaccine candidate to combat the Salmonella infection.Type: ApplicationFiled: August 22, 2008Publication date: May 19, 2011Applicant: Indian Institute of ScienceInventors: Dipshikha Chakravortty, Vidya Devi Negi
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Publication number: 20110105386Abstract: Methods and compositions for treating microbial infections associated with an emergence of resistance of a pathogenic microorganism to an antimicrobial agent, following treatment with antimicrobial agent are disclosed. The methods are effected by using a polymer which exhibits antimicrobial re-sensitizing activity, for re-sensitizing the pathogenic microorganisms to the antimicrobial agent, in combination with the antimicrobial agent. Further disclosed are novel polymers having an antimicrobial re-sensitizing activity.Type: ApplicationFiled: January 15, 2009Publication date: May 5, 2011Applicant: Technion Research & Development Foundation Ltd.Inventors: Amram Mor, Fadia Zaknoon, Shahar Rotem, Hadar Sarig
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Publication number: 20110105376Abstract: A method of making a Bacillus alpha-amylase variant that increases alpha-amylase production and the alpha-amy-lases produced thereby. The recombinant alpha-amylases can be placed in compositions and used for purposes of laundry detergents, cleaning and dishwashing detergents, fabric desizing, starch liquefaction, cereal liquefaction, starch saccharification, biofilm removal, and starch hydrolysis in cane sugar processing.Type: ApplicationFiled: March 20, 2008Publication date: May 5, 2011Inventors: George England, Marc Kolkman, Casper Vroemen
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Publication number: 20110081708Abstract: An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5?-UTR, ORF and 3?-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.Type: ApplicationFiled: September 30, 2010Publication date: April 7, 2011Applicant: GENSCRIPT HOLDINGS (HONG KONG) LIMITEDInventors: Xiaowu LIU, Yun HE, Zhuying WANG, Chunjiao WANG, Zhibing LIU, Tianhui XIA, Luquan WANG, Fang Liang ZHANG
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Patent number: 7919081Abstract: The present disclosure describes pathogenic bacteria that have been modified to be deficient in NiFe hydrogenase activity; compositions comprising such modified bacteria, and the use of such bacteria to protect animals from pathogenic enteric bacterial infections.Type: GrantFiled: February 28, 2005Date of Patent: April 5, 2011Assignee: University of Georgia Research Foundation, Inc.Inventors: Robert J. Maier, John S. Gunn
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Patent number: 7901924Abstract: Methods of increasing the cellular pool of A-CoA and thus driving the metabolic pathways in the direction of A-CoA containing metabolites by overexpressing rate limiting enzymes in A-CoA synthesis. Methods of increasing intracellular levels of CoA and A-CoA through genetic engineering of bacterial strains in conjunction with supplementation with precursor molecules.Type: GrantFiled: December 19, 2008Date of Patent: March 8, 2011Assignee: Rice UniversityInventors: Ka-Yiu San, George Nelson Bennett, Ravishankar V. Vadali
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Publication number: 20110053224Abstract: The invention describes a novel composition of matter obtained from the leaves of green plants, which is useful in promoting the growth of beneficial microorganisms. Specifically, that the invention describes a hydrolysate prepared from plant leaf biomass (leaf biomass hydrolysate or ‘LBH’) which dramatically stimulates the growth of beneficial microorganisms. Use of LBH as a fermentation substrate can also stimulate rapid production of organic acids and other organic compounds. LBH can be used as a substrate to promote the fermentation-based production of biobased industrial chemicals or biofuels, LBH can be utilized as a prebiotic to promote the growth of beneficial probiotic organisms, hi addition, LBH may also be useful in stimulating the fermentation-based production of other products, examples of which include preservatives, antibiotics, antigens, vaccines, amino acids, vitamins, recombinant proteins, bioremediation treatments, and immobilized enzymes.Type: ApplicationFiled: January 26, 2009Publication date: March 3, 2011Inventors: Yangming Martin Lo, Irene N.N. Yossa, Neil A. Belson
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Patent number: 7893231Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.Type: GrantFiled: October 25, 2007Date of Patent: February 22, 2011Assignee: Evonik Degussa GmbHInventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
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Publication number: 20110020869Abstract: Methods of increasing cultured cell growth yields and/or protein production from bacterial cell cultures are provided. More particularly, mutant bacterial cells having an alteration in the expression or activity of the cidABC operon, a gene therein, or a homolog or a regulator thereof, and methods for reducing acetic acid/acetate production in cultures are provided, as are methods for increasing cultured cell growth yields and/or protein production employing such cells. Methods for enhancing bacterial cell death and methods for identifying agents that increase the susceptibility of bacteria to cell death are also provided.Type: ApplicationFiled: September 29, 2010Publication date: January 27, 2011Inventors: Kenneth W. Bayles, Toni G. Patton, Kelly C. Rice
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Publication number: 20110021610Abstract: Nucleic acid molecules from hop (Humulus lupulus) have been isolated and characterized wherein said nucleic acid molecules encode polypeptides having aromatic prenyltransferase activity Expression or over-expression of said nucleic acid molecules alters the level of terpenophenolic compounds The polypeptides may be used in vivo or in vitro to produce terpenophenolic compounds (e g, prenylated acylphloroglucmols and prenylflavonoids) such as prenyl-PIVP, prenyl-PIBP, humulone, lupulone, desmethylxanthohumol and xanthohumolType: ApplicationFiled: March 16, 2009Publication date: January 27, 2011Applicant: NATIONAL RESEARCH COUNCIL OF CANADAInventors: Jonathan Page, Enwu Liu, Jana Nagel
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Patent number: 7871808Abstract: The present invention provides a method for producing an amino acid selected from the group consisting of L-alanine, L-valine, L-leucine, L-isoleucine, L-methionine, L-phenylalanine, L-proline, glycine, L-serine, L-threonine, L-cysteine, L-tyrosine, L-asparagine, L-glutamine, L-lysine, L-histidine, L-arginine, L-aspartic acid and L-glutamic acid and useful as medicament, chemical agent, food material and feed additive at high industrial efficiency, the method comprising culturing a microorganism having an ability to produce the amino acid and having resistance to an aminoquinoline derivative in a medium, producing and accumulating the amino acid in the present invention in the culture, and recovering the amino acid from the culture.Type: GrantFiled: May 1, 2006Date of Patent: January 18, 2011Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Kuniki Kino, Tetsuya Abe
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Publication number: 20110008801Abstract: The invention provides an atomic force microscope-based bioassay, assisted by thermodynamic characterizations to quickly and accurately screen for compounds that disrupt cell-cell or cell-substrate interactions.Type: ApplicationFiled: March 20, 2007Publication date: January 13, 2011Applicant: Worcester Polytechnic InstituteInventors: Terri A. Camesano, Yatao Liu, Amparo Gallardo-Moreno
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Publication number: 20110010806Abstract: The present disclosure relates to a method of directing the evolution of an organism by modifying the mutation rate of an organism. The increase in genetic diversity may be used to facilitate the selection of a desired hereditary trait in an organism.Type: ApplicationFiled: June 10, 2009Publication date: January 13, 2011Applicant: NEO-MORGAN LABORATORY INCORPORATEDInventors: Masanori Ogawa, Takayuki Horiuchi, Kazufumi Tabata, Shuntaro Yano, Michi Kubota, Eri Kurita, Akiko Itadani, Sanae Fukushima-Tanaka
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Publication number: 20110008782Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.Type: ApplicationFiled: August 20, 2010Publication date: January 13, 2011Applicant: The Scripps Research InstituteInventors: Lital Alfonta, Peter G. Schultz, Zhiwen Zhang
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Publication number: 20110008449Abstract: The present invention relates to human specific Escherichia coli strains.Type: ApplicationFiled: August 13, 2008Publication date: January 13, 2011Inventors: Olivier Clermont, Erick Denamur, Tony Le Gall, Olivier Tenaillon
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Publication number: 20100330116Abstract: The present invention relates to live attenuated Salmonella cultures for use as vaccines. The Salmonella cultures of the present invention have a substantially reduced capacity to grow and replicate in the presence of bile. The reduced capacity for growth is due to a metabolic-drift mutation induced by exposure to a combination of nalidixic acid and rifampicin for a time and under conditions sufficient to induce the mutation.Type: ApplicationFiled: April 28, 2010Publication date: December 30, 2010Applicant: The University of QueenslandInventor: Tetsuo Mizuno
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Patent number: 7858341Abstract: The expression vectors and methods using an E. coli expression system for the large scale production of FGF18 are described. The vectors utilize the FGF18 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the FGF18 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains, as well as OmpT and fhuA negative strains transformed with an FGF18 expression vector.Type: GrantFiled: December 12, 2005Date of Patent: December 28, 2010Assignee: ZymoGenetics, Inc.Inventors: Brian J. Reardon, Susan H. Julien, Chung-leung Chan, Hong Y. Liu
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Publication number: 20100322955Abstract: A series of genes from Salmonella typhimurium are shown to encode products which are implicated in virulence. The identification of these genes therefore allows attenuated microorganisms to be produced. Furthermore, the genes or their encoded products can be used in the manufacture of vaccines for therapeutic application.Type: ApplicationFiled: May 28, 2009Publication date: December 23, 2010Inventor: Steven Neville Chatfield
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Publication number: 20100317086Abstract: A new culture method for producing high levels of a metabolite, such as succinic acid uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having <5% partial pressure of oxygen, and the production of high levels of succinate under oxygen deprived conditions. The method can be performed in a single reactor, and is amenable to efficient scale up.Type: ApplicationFiled: December 28, 2007Publication date: December 16, 2010Applicants: ROQUETTE FRERES, WILLIAM MARSH RICE UNIVERSITYInventors: Laurent Segueilha, Ka-Yui San, George Bennett, Irene Martinez
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Publication number: 20100311134Abstract: The present invention provides a method of producing sclareol, said method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, said method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing said nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.Type: ApplicationFiled: January 29, 2008Publication date: December 9, 2010Applicant: FIRMENICH SAInventor: Michel Schalk
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Publication number: 20100311135Abstract: The invention provides: an isopropyl alcohol-producing bacterium which has an acetoacetate decarboxylase activity, an isopropyl alcohol dehydrogenase activity, a CoA transferase activity and a thiolase activity having been imparted thereto and is capable of producing isopropyl alcohol from a plant-derived material; a method of producing isopropyl alcohol whereby isopropyl alcohol is produced from a plant-derived material by using this isopropyl alcohol-producing bacterium; and an apparatus therefor.Type: ApplicationFiled: July 4, 2008Publication date: December 9, 2010Inventors: Nozomi Takebayashi, Mitsufumi Wada, Daisuke Mochizuki, Fuminobu Yoshimi, Seiichi Watanabe, Hitoshi Takahashi, Takashi Morishige
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Publication number: 20100311146Abstract: A mixing vessel (10) for containing a liquid, comprises a chamber having a lower chamber portion and an upper chamber portion wider than the lower portion, gas inlet means (14) for supplying gas to the lower portion, liquid circulation inducing means (17) and an inclined abutment (24), such that, when gas is supplied via the gas inlet means gas in the form of bubbles initially rises substantially vertically in liquid and is redirected in a substantially horizontal direction by the abutment.Type: ApplicationFiled: June 11, 2008Publication date: December 9, 2010Applicant: Cellexus LimitedInventor: Kevin Auton
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Publication number: 20100304450Abstract: Microbial production of pyruvate and metabolites derived from pyruvate in cells exhibiting reduced pyruvate dehydrogenase activity compared to wild-type cells. Acetate and glucose are supplied as a carbon sources.Type: ApplicationFiled: June 28, 2010Publication date: December 2, 2010Applicant: University of Georgia Research Foundation, Inc.Inventors: Mark A. EITEMAN, Elliot Altman, Yihui Zhu
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Publication number: 20100290996Abstract: This invention is directed to applying a low sedimental fluid shear environment to manipulate microorganisms, and to microorganisms and compositions obtained based on such manipulation. Specifically, the present invention provides methods of modifying a molecular genetic or phenotypic characteristic (e.g., virulence, stress resistance or biofilm formation) of a microorganism by culturing in a low sedimental shear environment. One or more ion concentrations in the culture can be modulated in order to inhibit or amplify the extent of the modification. The present invention also provides microorganisms obtained from a low sedimental shear culture, which exhibit modified and desirable phenotypic characteristics, as well as therapeutic, vaccine and bioindustrial products prepared from such microorganisms.Type: ApplicationFiled: September 10, 2008Publication date: November 18, 2010Applicant: Arizona Board of Regents, for and on behalf of Arizona State UniversityInventors: Cheryl A. Nickerson, James W. Wilson, Mark C. Ott, Eric A. Nauman, Michael J. Schurr, Mayra A. Nelman-Gonzalez, Shameema Sarker
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Publication number: 20100292136Abstract: The present invention relates to a polymyxin derivative wherein R1, R2 and R3 are optional and R1, R2, R3, R5, R8 and R9 are cationic or neutral amino acid residues selected so that the total number of positive charges at physiological pH is at least two but no more than three; and to a combination product comprising at least two such derivatives.Type: ApplicationFiled: July 23, 2010Publication date: November 18, 2010Applicant: Northern Antibiotics OYInventors: Martti Sakari VAARA, Timo Ilmari Vaara
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Publication number: 20100279362Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ybiV gene.Type: ApplicationFiled: January 30, 2008Publication date: November 4, 2010Inventors: Konstantin Vyacheslavovich Rybak, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov
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Patent number: 7824893Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate alkynyl amino acids such as para-propargyloxyphenylalanine into proteins produced in a eubacteria host such as E. coli. The invention provides novel orthoghonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing alkynyl amino acids, and cellular translation systems.Type: GrantFiled: September 20, 2005Date of Patent: November 2, 2010Assignee: The Scripps Research InstituteInventors: Alexander Deiters, Peter G. Schultz
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Publication number: 20100273194Abstract: Provided herein are immunoreactive peptides which can selectively bind Ehrlichia-specific anti-p120 or anti-p140 antibodies. Methods and kits utilizing the immunoreactive peptides are also provided. The immunoreactive peptides may be utilized, e.g., for determining whether or not a subject is infected with Ehrlichia chaffeensis or Ehrlichia canis. In certain embodiments, the immunoreactive peptides may be utilized in an ELISA or lateral flow assay.Type: ApplicationFiled: April 28, 2010Publication date: October 28, 2010Inventors: Jere W. McBride, Tian Luo
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Publication number: 20100267147Abstract: Site-specific mutation in methylated circular stranded DNA molecules is conferred by mutagenic primer pairs and methylase deficient Escherichia coli. The mutagenic primer pairs are complementary at 5? end or 3? end, or completely complementary to each other. Firstly, mutagenic primer pair is annealed to opposite strands of the methylated circular double-stranded parent DNA molecules. Then, polymerase chain reaction is performed by using unmethylated dNTPs to create unmethylated mutagenized double-stranded daughter DNA molecules. Finally, the reaction mixture of the methylated parent DNA molecules and unmethylated mutagenized daughter DNA molecules is transformed into a methylase deficient E. coli. The replication of methylated parent DNA is inhibited in methylase deficient host cell. In contrast, the unmethylated daughter DNA, which contains the desired mutation, are efficiently replicated in methylase deficient host cell and recovered thereafter.Type: ApplicationFiled: June 3, 2010Publication date: October 21, 2010Applicant: GM Biosciences, Inc.Inventor: Miao Qiao
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Publication number: 20100267094Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.Type: ApplicationFiled: June 28, 2010Publication date: October 21, 2010Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
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Publication number: 20100267816Abstract: A human anti-amyloid ? peptide (hereinafter referred to as “A?”) antibody that binds to A? to thereby inhibit aggregation of A? molecules, and a fragment of said antibody are provided. The antibody and a fragment thereof according to the present invention, comprising a variable region of a human-derived anti-A? antibody, strongly reacts with A? to thereby inhibit its aggregation and hence may be used as a medicament for the prophylaxis and treatment of Alzheimer dementia.Type: ApplicationFiled: June 2, 2010Publication date: October 21, 2010Applicant: JURIDICAL FDN THE CHEMO-SERO-THERAPEUTIC RES INSTInventors: Kazuhisa SUGIMURA, Toshihiro Nakashima
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Publication number: 20100255021Abstract: The invention relates to a truncated L1 protein of the Human Papillomavirus Type 6, a virus-like particle consisting of the protein, a vaccine comprising said virus-like particle, and the use of the vaccine in the prevention of condyloma acuminatum or HPV infections.Type: ApplicationFiled: May 29, 2008Publication date: October 7, 2010Applicants: Xiamen University, Beijing Wantai Biological Pharmacy Enterprise Co., Ltd.Inventors: Shaowei Li, Huirong Pan, Bo Liu, Jun Zhang, Ji Miao, Ningshao Xia
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Patent number: 7807441Abstract: A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.Type: GrantFiled: August 11, 2005Date of Patent: October 5, 2010Assignee: BN Immunotherapeutics, Inc.Inventors: Lucilla Steinaa, Søren Mouritsen, Anand Gautam, Iben Dalum, Jesper Haaning, Dana Leach, Klaus Gregorius Nielsen, Gunilla Karlsson, Peter Birk Rasmussen
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Patent number: 7790422Abstract: A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.Type: GrantFiled: April 9, 2007Date of Patent: September 7, 2010Assignee: Ajinomoto Co., Inc.Inventors: Ryo Takeshita, Shinichi Sugimoto
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Patent number: 7790416Abstract: The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.Type: GrantFiled: April 5, 2007Date of Patent: September 7, 2010Assignee: Rice UniversityInventors: Ka-Yiu San, George N. Bennett, Ailen Sanchez
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Patent number: 7790435Abstract: Quiescence is induced in cells using indole compounds. Expression continues from extra-chromosomal vectors within the cells during quiescence, while chromosomal expression is suppressed. The cells may be used as factories for the production of large amounts of polypeptides of interest, particularly polypeptides which normally have an adverse effect on cell viability or growth. Expression from an extra-chromosomal vector of interest may be monitored, in view of the reduced background expression from the chromosome. Vector copy number may be amplified. Cell cycles may be synchronized.Type: GrantFiled: December 18, 2006Date of Patent: September 7, 2010Assignee: Cambridge Microbial Technologies LimitedInventors: David Keith Summers, Ellie Chant