Escherichia (e.g., E. Coli, Etc.) Or Salmonella Patents (Class 435/252.8)
  • Patent number: 8003091
    Abstract: The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011 minicells.
    Type: Grant
    Filed: March 27, 2007
    Date of Patent: August 23, 2011
    Assignee: Engeneic Molecular Delivery Pty. Ltd.
    Inventors: Himanshu Brahmbhatt, Jennifer MacDiarmid
  • Publication number: 20110195483
    Abstract: Compositions and methods of producing vaccines, including methods wherein whole organism vaccines are provided with limited replication abilities, thereby increasing vaccine safety and efficacy, through the use of non-natural, unnatural, or non-naturally encoded amino acids.
    Type: Application
    Filed: September 28, 2009
    Publication date: August 11, 2011
    Applicant: AMBRX, INC.
    Inventors: Feng Tian, Brad Hehli
  • Publication number: 20110177579
    Abstract: An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40±1 kDa. The gene encoding the enzyme was cloned and sequenced, and found to have significant sequence homology to known zinc-containing ADHs and L-threonine dehydrogenases with both binding motifs of catalytic zinc and NADP+. The wild-type enzyme is a primary-secondary ADH that exhibits a substrate preference for secondary alcohols and corresponding ketones, and exhibits unusual stereoselectivity. The wild-type enzyme was found to have outstanding thermostability, demonstrating 60% activity after incubation at 80° C. for 40 hours. Site-directed mutagenesis was used to substitute the cyteine residue at position 56 with a serine, to provide the TgADH(C56S) mutant. In the assays that we carried out, we found virtually no difference in enzyme activity and oxygen-sensitivity between the mutant TgADH (C56S) and wild type TgADH.
    Type: Application
    Filed: March 25, 2011
    Publication date: July 21, 2011
    Inventors: Kesen MA, Xiangxian YING
  • Patent number: 7981411
    Abstract: The present invention relates to the use of F4+ non-pathogenic Escherichia coli strains to promote growth in an animal. The present invention also relates to the use of such strains to homogenize growth among a herd of animals. More specifically, the animal(s) of interest in the present invention are those wherein growth promotion or growth homogenization are desired goals, such as animals reared for meat production. The present invention further relates to a method for promoting growth of an animal as well as a method for homogenizing growth among a herd of animals.
    Type: Grant
    Filed: February 3, 2005
    Date of Patent: July 19, 2011
    Assignee: Valorisation—Recherche, Limited Partnership
    Inventors: Eric Nadeau, John Morris Fairbrother
  • Publication number: 20110171703
    Abstract: The present invention provides: a lactic acid-producing Escherichia coli including an enzymatic activity of at least one NAD-dependent lactate dehydrogenase and an enzymatic activity of at least one NAD-independent lactate oxidoreductase, both of which are enhanced so as to decompose one of D-lactic acid or L-lactic acid and to produce the other one of D-lactic acid or L-lactic acid; and a lactic acid production method using the lactic acid-producing Escherichia coli.
    Type: Application
    Filed: September 11, 2009
    Publication date: July 14, 2011
    Inventors: Mitsufumi Wada, Katsuyuki Takahashi, Takashi Morishige, Daisuke Miyazawa, Hitoshi Takahashi, Daisuke Mochizuki, Tadashi Araki
  • Patent number: 7972812
    Abstract: A novel cytokine designated TRAIL induces apoptosis of certain target cells, including cancer cells and virally infected cells. Isolated DNA sequences encoding TRAIL are disclosed, along with expression vectors and transformed host cells useful in producing TRAIL polypeptides. Antibodies that specifically bind TRAIL are provided as well.
    Type: Grant
    Filed: May 13, 2010
    Date of Patent: July 5, 2011
    Assignee: Immunex Corporation
    Inventors: Steven R. Wiley, Raymond G. Goodwin
  • Publication number: 20110159515
    Abstract: The invention relates to assay methods for use in detecting specific materials such as core oligosaccharides derived from microorganisms, particularly pathogenic microorganisms, in a test sample. The invention further relates to compositions and methods for the rapid growth of such microorganisms enabling detection of same significantly earlier than is currently possible. In particular embodiments the invention is directed towards the rapid growth and/or detection of Salmonella, Shigella or Listeria.
    Type: Application
    Filed: September 10, 2009
    Publication date: June 30, 2011
    Applicant: SOLUS SCIENTIFIC SOLUTIONS LIMITED
    Inventor: William Stimson
  • Patent number: 7968325
    Abstract: Coexpression of a polyhydroxyalkanoic acid synthase and a either a fatty acid:acyl-CoA transferase or an acyl-CoA synthetase in cells enables the biosynthesis of polyester materials. Plasmids, bacteria, materials, and methods for the preparation of polyesters are disclosed.
    Type: Grant
    Filed: June 14, 2004
    Date of Patent: June 28, 2011
    Assignee: Metabolix, Inc.
    Inventors: Silke Hein, Brigitte Sohling, Gerhard Gottschalk, Alexander Steinbuchel
  • Publication number: 20110151511
    Abstract: The present invention provides novel rapidly growing microorganisms and methods for their use in cloning or subcloning nucleic acid molecules. The rapid growing microorganisms of the present invention form colonies more rapidly than microorganisms typically used in molecular biology and thus provide a significant improvement in in vitro cloning methods used extensively in molecular biology. The invention also relates to kits and compositions used in the methods of the invention.
    Type: Application
    Filed: December 17, 2010
    Publication date: June 23, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Frederic R. Bloom, Brian J. Schmidt, Jhy-Jhu Lin
  • Publication number: 20110150907
    Abstract: The present disclosure relates generally to recombinant bacteria (e.g., Lactobacillus) that express one or more binding peptides, antibodies and/or antibody binding fragments on their surface that are specific for one or more pathogens and/or toxins, including toxins from pathogens. The recombinant bacteria may be used for binding, removing and/or neutralizing one or more pathogens and/or toxins, including toxins from pathogens in a gastrointestinal tract.
    Type: Application
    Filed: April 3, 2009
    Publication date: June 23, 2011
    Applicant: FARALLONE HOLDINGS BV
    Inventors: Jos Seegers, Stephen F. Carroll
  • Publication number: 20110142879
    Abstract: Methods of preparing a DNA comprising sequences derived from the genomic RNA (gRNA) of a coronavirus are provided, comprising cloning under the expression of a promoter a coronavirus interfering defective genome into a bacterial artificial chromosome (BAC) and reinserting into said genome the deleted sequences within the defective genome, wherein said sequences have a homology of at least 60% to the natural sequence of the virus, wherein said sequences code for an RNA-dependent RNA polymerase and at least one structural or non-structural protein, and wherein a fragment of said DNA is capable of being transcribed into RNA and assembeld to a virion. Also provided are infective clones, recombinant viral vectors, and vaccines.
    Type: Application
    Filed: November 3, 2010
    Publication date: June 16, 2011
    Applicant: CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
    Inventor: LUIS ENJUANES SANCHEZ
  • Patent number: 7960141
    Abstract: The invention relates to compositions and methods for the in vivo production of polypeptides comprising one or more unnatural amino acids. Specifically, the invention provides plasmid systems for the efficient eubacterial expression of polypeptides comprising one or more unnatural amino acids at genetically-programmed positions.
    Type: Grant
    Filed: March 7, 2007
    Date of Patent: June 14, 2011
    Assignee: The Scripps Research Institute
    Inventors: Youngha Ryu, Peter G. Schultz
  • Publication number: 20110136211
    Abstract: Disclosed is an apparatus for culturing microorganisms and methods for using the same.
    Type: Application
    Filed: December 8, 2009
    Publication date: June 9, 2011
    Inventor: Qi Zhong
  • Patent number: 7947440
    Abstract: The present invention provides a method for measuring the specific rate of ribosome synthesis for a distinct cell population, such as a distinct microbial population. For an actively growing (or non-growing) culture, the specific rate of ribosome synthesis is identical to the specific growth rate of the culture. With the method of the invention, researchers will be able to measure the specific growth rate of distinct cell populations in mixed cultures, such as biological reactor systems or environmental samples. In addition, the method of the invention provides the ability to identify members of a distinct cell population that are rapidly growing.
    Type: Grant
    Filed: September 15, 2006
    Date of Patent: May 24, 2011
    Assignee: University of South Florida
    Inventors: Peter George Stroot, Matthew Raymond Cutter
  • Publication number: 20110117132
    Abstract: The present invention relates to an attenuated Salmonella typhi having mutation in chromosomal gene loci, its use as a potent vaccine candidate to combat the Salmonella infection.
    Type: Application
    Filed: August 22, 2008
    Publication date: May 19, 2011
    Applicant: Indian Institute of Science
    Inventors: Dipshikha Chakravortty, Vidya Devi Negi
  • Publication number: 20110105386
    Abstract: Methods and compositions for treating microbial infections associated with an emergence of resistance of a pathogenic microorganism to an antimicrobial agent, following treatment with antimicrobial agent are disclosed. The methods are effected by using a polymer which exhibits antimicrobial re-sensitizing activity, for re-sensitizing the pathogenic microorganisms to the antimicrobial agent, in combination with the antimicrobial agent. Further disclosed are novel polymers having an antimicrobial re-sensitizing activity.
    Type: Application
    Filed: January 15, 2009
    Publication date: May 5, 2011
    Applicant: Technion Research & Development Foundation Ltd.
    Inventors: Amram Mor, Fadia Zaknoon, Shahar Rotem, Hadar Sarig
  • Publication number: 20110105376
    Abstract: A method of making a Bacillus alpha-amylase variant that increases alpha-amylase production and the alpha-amy-lases produced thereby. The recombinant alpha-amylases can be placed in compositions and used for purposes of laundry detergents, cleaning and dishwashing detergents, fabric desizing, starch liquefaction, cereal liquefaction, starch saccharification, biofilm removal, and starch hydrolysis in cane sugar processing.
    Type: Application
    Filed: March 20, 2008
    Publication date: May 5, 2011
    Inventors: George England, Marc Kolkman, Casper Vroemen
  • Publication number: 20110081708
    Abstract: An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5?-UTR, ORF and 3?-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.
    Type: Application
    Filed: September 30, 2010
    Publication date: April 7, 2011
    Applicant: GENSCRIPT HOLDINGS (HONG KONG) LIMITED
    Inventors: Xiaowu LIU, Yun HE, Zhuying WANG, Chunjiao WANG, Zhibing LIU, Tianhui XIA, Luquan WANG, Fang Liang ZHANG
  • Patent number: 7919081
    Abstract: The present disclosure describes pathogenic bacteria that have been modified to be deficient in NiFe hydrogenase activity; compositions comprising such modified bacteria, and the use of such bacteria to protect animals from pathogenic enteric bacterial infections.
    Type: Grant
    Filed: February 28, 2005
    Date of Patent: April 5, 2011
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Robert J. Maier, John S. Gunn
  • Patent number: 7901924
    Abstract: Methods of increasing the cellular pool of A-CoA and thus driving the metabolic pathways in the direction of A-CoA containing metabolites by overexpressing rate limiting enzymes in A-CoA synthesis. Methods of increasing intracellular levels of CoA and A-CoA through genetic engineering of bacterial strains in conjunction with supplementation with precursor molecules.
    Type: Grant
    Filed: December 19, 2008
    Date of Patent: March 8, 2011
    Assignee: Rice University
    Inventors: Ka-Yiu San, George Nelson Bennett, Ravishankar V. Vadali
  • Publication number: 20110053224
    Abstract: The invention describes a novel composition of matter obtained from the leaves of green plants, which is useful in promoting the growth of beneficial microorganisms. Specifically, that the invention describes a hydrolysate prepared from plant leaf biomass (leaf biomass hydrolysate or ‘LBH’) which dramatically stimulates the growth of beneficial microorganisms. Use of LBH as a fermentation substrate can also stimulate rapid production of organic acids and other organic compounds. LBH can be used as a substrate to promote the fermentation-based production of biobased industrial chemicals or biofuels, LBH can be utilized as a prebiotic to promote the growth of beneficial probiotic organisms, hi addition, LBH may also be useful in stimulating the fermentation-based production of other products, examples of which include preservatives, antibiotics, antigens, vaccines, amino acids, vitamins, recombinant proteins, bioremediation treatments, and immobilized enzymes.
    Type: Application
    Filed: January 26, 2009
    Publication date: March 3, 2011
    Inventors: Yangming Martin Lo, Irene N.N. Yossa, Neil A. Belson
  • Patent number: 7893231
    Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.
    Type: Grant
    Filed: October 25, 2007
    Date of Patent: February 22, 2011
    Assignee: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
  • Publication number: 20110020869
    Abstract: Methods of increasing cultured cell growth yields and/or protein production from bacterial cell cultures are provided. More particularly, mutant bacterial cells having an alteration in the expression or activity of the cidABC operon, a gene therein, or a homolog or a regulator thereof, and methods for reducing acetic acid/acetate production in cultures are provided, as are methods for increasing cultured cell growth yields and/or protein production employing such cells. Methods for enhancing bacterial cell death and methods for identifying agents that increase the susceptibility of bacteria to cell death are also provided.
    Type: Application
    Filed: September 29, 2010
    Publication date: January 27, 2011
    Inventors: Kenneth W. Bayles, Toni G. Patton, Kelly C. Rice
  • Publication number: 20110021610
    Abstract: Nucleic acid molecules from hop (Humulus lupulus) have been isolated and characterized wherein said nucleic acid molecules encode polypeptides having aromatic prenyltransferase activity Expression or over-expression of said nucleic acid molecules alters the level of terpenophenolic compounds The polypeptides may be used in vivo or in vitro to produce terpenophenolic compounds (e g, prenylated acylphloroglucmols and prenylflavonoids) such as prenyl-PIVP, prenyl-PIBP, humulone, lupulone, desmethylxanthohumol and xanthohumol
    Type: Application
    Filed: March 16, 2009
    Publication date: January 27, 2011
    Applicant: NATIONAL RESEARCH COUNCIL OF CANADA
    Inventors: Jonathan Page, Enwu Liu, Jana Nagel
  • Patent number: 7871808
    Abstract: The present invention provides a method for producing an amino acid selected from the group consisting of L-alanine, L-valine, L-leucine, L-isoleucine, L-methionine, L-phenylalanine, L-proline, glycine, L-serine, L-threonine, L-cysteine, L-tyrosine, L-asparagine, L-glutamine, L-lysine, L-histidine, L-arginine, L-aspartic acid and L-glutamic acid and useful as medicament, chemical agent, food material and feed additive at high industrial efficiency, the method comprising culturing a microorganism having an ability to produce the amino acid and having resistance to an aminoquinoline derivative in a medium, producing and accumulating the amino acid in the present invention in the culture, and recovering the amino acid from the culture.
    Type: Grant
    Filed: May 1, 2006
    Date of Patent: January 18, 2011
    Assignee: Kyowa Hakko Bio Co., Ltd.
    Inventors: Kuniki Kino, Tetsuya Abe
  • Publication number: 20110008801
    Abstract: The invention provides an atomic force microscope-based bioassay, assisted by thermodynamic characterizations to quickly and accurately screen for compounds that disrupt cell-cell or cell-substrate interactions.
    Type: Application
    Filed: March 20, 2007
    Publication date: January 13, 2011
    Applicant: Worcester Polytechnic Institute
    Inventors: Terri A. Camesano, Yatao Liu, Amparo Gallardo-Moreno
  • Publication number: 20110010806
    Abstract: The present disclosure relates to a method of directing the evolution of an organism by modifying the mutation rate of an organism. The increase in genetic diversity may be used to facilitate the selection of a desired hereditary trait in an organism.
    Type: Application
    Filed: June 10, 2009
    Publication date: January 13, 2011
    Applicant: NEO-MORGAN LABORATORY INCORPORATED
    Inventors: Masanori Ogawa, Takayuki Horiuchi, Kazufumi Tabata, Shuntaro Yano, Michi Kubota, Eri Kurita, Akiko Itadani, Sanae Fukushima-Tanaka
  • Publication number: 20110008782
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.
    Type: Application
    Filed: August 20, 2010
    Publication date: January 13, 2011
    Applicant: The Scripps Research Institute
    Inventors: Lital Alfonta, Peter G. Schultz, Zhiwen Zhang
  • Publication number: 20110008449
    Abstract: The present invention relates to human specific Escherichia coli strains.
    Type: Application
    Filed: August 13, 2008
    Publication date: January 13, 2011
    Inventors: Olivier Clermont, Erick Denamur, Tony Le Gall, Olivier Tenaillon
  • Publication number: 20100330116
    Abstract: The present invention relates to live attenuated Salmonella cultures for use as vaccines. The Salmonella cultures of the present invention have a substantially reduced capacity to grow and replicate in the presence of bile. The reduced capacity for growth is due to a metabolic-drift mutation induced by exposure to a combination of nalidixic acid and rifampicin for a time and under conditions sufficient to induce the mutation.
    Type: Application
    Filed: April 28, 2010
    Publication date: December 30, 2010
    Applicant: The University of Queensland
    Inventor: Tetsuo Mizuno
  • Patent number: 7858341
    Abstract: The expression vectors and methods using an E. coli expression system for the large scale production of FGF18 are described. The vectors utilize the FGF18 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the FGF18 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains, as well as OmpT and fhuA negative strains transformed with an FGF18 expression vector.
    Type: Grant
    Filed: December 12, 2005
    Date of Patent: December 28, 2010
    Assignee: ZymoGenetics, Inc.
    Inventors: Brian J. Reardon, Susan H. Julien, Chung-leung Chan, Hong Y. Liu
  • Publication number: 20100322955
    Abstract: A series of genes from Salmonella typhimurium are shown to encode products which are implicated in virulence. The identification of these genes therefore allows attenuated microorganisms to be produced. Furthermore, the genes or their encoded products can be used in the manufacture of vaccines for therapeutic application.
    Type: Application
    Filed: May 28, 2009
    Publication date: December 23, 2010
    Inventor: Steven Neville Chatfield
  • Publication number: 20100317086
    Abstract: A new culture method for producing high levels of a metabolite, such as succinic acid uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having <5% partial pressure of oxygen, and the production of high levels of succinate under oxygen deprived conditions. The method can be performed in a single reactor, and is amenable to efficient scale up.
    Type: Application
    Filed: December 28, 2007
    Publication date: December 16, 2010
    Applicants: ROQUETTE FRERES, WILLIAM MARSH RICE UNIVERSITY
    Inventors: Laurent Segueilha, Ka-Yui San, George Bennett, Irene Martinez
  • Publication number: 20100311134
    Abstract: The present invention provides a method of producing sclareol, said method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, said method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing said nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.
    Type: Application
    Filed: January 29, 2008
    Publication date: December 9, 2010
    Applicant: FIRMENICH SA
    Inventor: Michel Schalk
  • Publication number: 20100311135
    Abstract: The invention provides: an isopropyl alcohol-producing bacterium which has an acetoacetate decarboxylase activity, an isopropyl alcohol dehydrogenase activity, a CoA transferase activity and a thiolase activity having been imparted thereto and is capable of producing isopropyl alcohol from a plant-derived material; a method of producing isopropyl alcohol whereby isopropyl alcohol is produced from a plant-derived material by using this isopropyl alcohol-producing bacterium; and an apparatus therefor.
    Type: Application
    Filed: July 4, 2008
    Publication date: December 9, 2010
    Inventors: Nozomi Takebayashi, Mitsufumi Wada, Daisuke Mochizuki, Fuminobu Yoshimi, Seiichi Watanabe, Hitoshi Takahashi, Takashi Morishige
  • Publication number: 20100311146
    Abstract: A mixing vessel (10) for containing a liquid, comprises a chamber having a lower chamber portion and an upper chamber portion wider than the lower portion, gas inlet means (14) for supplying gas to the lower portion, liquid circulation inducing means (17) and an inclined abutment (24), such that, when gas is supplied via the gas inlet means gas in the form of bubbles initially rises substantially vertically in liquid and is redirected in a substantially horizontal direction by the abutment.
    Type: Application
    Filed: June 11, 2008
    Publication date: December 9, 2010
    Applicant: Cellexus Limited
    Inventor: Kevin Auton
  • Publication number: 20100304450
    Abstract: Microbial production of pyruvate and metabolites derived from pyruvate in cells exhibiting reduced pyruvate dehydrogenase activity compared to wild-type cells. Acetate and glucose are supplied as a carbon sources.
    Type: Application
    Filed: June 28, 2010
    Publication date: December 2, 2010
    Applicant: University of Georgia Research Foundation, Inc.
    Inventors: Mark A. EITEMAN, Elliot Altman, Yihui Zhu
  • Publication number: 20100290996
    Abstract: This invention is directed to applying a low sedimental fluid shear environment to manipulate microorganisms, and to microorganisms and compositions obtained based on such manipulation. Specifically, the present invention provides methods of modifying a molecular genetic or phenotypic characteristic (e.g., virulence, stress resistance or biofilm formation) of a microorganism by culturing in a low sedimental shear environment. One or more ion concentrations in the culture can be modulated in order to inhibit or amplify the extent of the modification. The present invention also provides microorganisms obtained from a low sedimental shear culture, which exhibit modified and desirable phenotypic characteristics, as well as therapeutic, vaccine and bioindustrial products prepared from such microorganisms.
    Type: Application
    Filed: September 10, 2008
    Publication date: November 18, 2010
    Applicant: Arizona Board of Regents, for and on behalf of Arizona State University
    Inventors: Cheryl A. Nickerson, James W. Wilson, Mark C. Ott, Eric A. Nauman, Michael J. Schurr, Mayra A. Nelman-Gonzalez, Shameema Sarker
  • Publication number: 20100292136
    Abstract: The present invention relates to a polymyxin derivative wherein R1, R2 and R3 are optional and R1, R2, R3, R5, R8 and R9 are cationic or neutral amino acid residues selected so that the total number of positive charges at physiological pH is at least two but no more than three; and to a combination product comprising at least two such derivatives.
    Type: Application
    Filed: July 23, 2010
    Publication date: November 18, 2010
    Applicant: Northern Antibiotics OY
    Inventors: Martti Sakari VAARA, Timo Ilmari Vaara
  • Publication number: 20100279362
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ybiV gene.
    Type: Application
    Filed: January 30, 2008
    Publication date: November 4, 2010
    Inventors: Konstantin Vyacheslavovich Rybak, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov
  • Patent number: 7824893
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate alkynyl amino acids such as para-propargyloxyphenylalanine into proteins produced in a eubacteria host such as E. coli. The invention provides novel orthoghonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing alkynyl amino acids, and cellular translation systems.
    Type: Grant
    Filed: September 20, 2005
    Date of Patent: November 2, 2010
    Assignee: The Scripps Research Institute
    Inventors: Alexander Deiters, Peter G. Schultz
  • Publication number: 20100273194
    Abstract: Provided herein are immunoreactive peptides which can selectively bind Ehrlichia-specific anti-p120 or anti-p140 antibodies. Methods and kits utilizing the immunoreactive peptides are also provided. The immunoreactive peptides may be utilized, e.g., for determining whether or not a subject is infected with Ehrlichia chaffeensis or Ehrlichia canis. In certain embodiments, the immunoreactive peptides may be utilized in an ELISA or lateral flow assay.
    Type: Application
    Filed: April 28, 2010
    Publication date: October 28, 2010
    Inventors: Jere W. McBride, Tian Luo
  • Publication number: 20100267147
    Abstract: Site-specific mutation in methylated circular stranded DNA molecules is conferred by mutagenic primer pairs and methylase deficient Escherichia coli. The mutagenic primer pairs are complementary at 5? end or 3? end, or completely complementary to each other. Firstly, mutagenic primer pair is annealed to opposite strands of the methylated circular double-stranded parent DNA molecules. Then, polymerase chain reaction is performed by using unmethylated dNTPs to create unmethylated mutagenized double-stranded daughter DNA molecules. Finally, the reaction mixture of the methylated parent DNA molecules and unmethylated mutagenized daughter DNA molecules is transformed into a methylase deficient E. coli. The replication of methylated parent DNA is inhibited in methylase deficient host cell. In contrast, the unmethylated daughter DNA, which contains the desired mutation, are efficiently replicated in methylase deficient host cell and recovered thereafter.
    Type: Application
    Filed: June 3, 2010
    Publication date: October 21, 2010
    Applicant: GM Biosciences, Inc.
    Inventor: Miao Qiao
  • Publication number: 20100267094
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Application
    Filed: June 28, 2010
    Publication date: October 21, 2010
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20100267816
    Abstract: A human anti-amyloid ? peptide (hereinafter referred to as “A?”) antibody that binds to A? to thereby inhibit aggregation of A? molecules, and a fragment of said antibody are provided. The antibody and a fragment thereof according to the present invention, comprising a variable region of a human-derived anti-A? antibody, strongly reacts with A? to thereby inhibit its aggregation and hence may be used as a medicament for the prophylaxis and treatment of Alzheimer dementia.
    Type: Application
    Filed: June 2, 2010
    Publication date: October 21, 2010
    Applicant: JURIDICAL FDN THE CHEMO-SERO-THERAPEUTIC RES INST
    Inventors: Kazuhisa SUGIMURA, Toshihiro Nakashima
  • Publication number: 20100255021
    Abstract: The invention relates to a truncated L1 protein of the Human Papillomavirus Type 6, a virus-like particle consisting of the protein, a vaccine comprising said virus-like particle, and the use of the vaccine in the prevention of condyloma acuminatum or HPV infections.
    Type: Application
    Filed: May 29, 2008
    Publication date: October 7, 2010
    Applicants: Xiamen University, Beijing Wantai Biological Pharmacy Enterprise Co., Ltd.
    Inventors: Shaowei Li, Huirong Pan, Bo Liu, Jun Zhang, Ji Miao, Ningshao Xia
  • Patent number: 7807441
    Abstract: A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.
    Type: Grant
    Filed: August 11, 2005
    Date of Patent: October 5, 2010
    Assignee: BN Immunotherapeutics, Inc.
    Inventors: Lucilla Steinaa, Søren Mouritsen, Anand Gautam, Iben Dalum, Jesper Haaning, Dana Leach, Klaus Gregorius Nielsen, Gunilla Karlsson, Peter Birk Rasmussen
  • Patent number: 7790422
    Abstract: A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
    Type: Grant
    Filed: April 9, 2007
    Date of Patent: September 7, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Ryo Takeshita, Shinichi Sugimoto
  • Patent number: 7790416
    Abstract: The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.
    Type: Grant
    Filed: April 5, 2007
    Date of Patent: September 7, 2010
    Assignee: Rice University
    Inventors: Ka-Yiu San, George N. Bennett, Ailen Sanchez
  • Patent number: 7790435
    Abstract: Quiescence is induced in cells using indole compounds. Expression continues from extra-chromosomal vectors within the cells during quiescence, while chromosomal expression is suppressed. The cells may be used as factories for the production of large amounts of polypeptides of interest, particularly polypeptides which normally have an adverse effect on cell viability or growth. Expression from an extra-chromosomal vector of interest may be monitored, in view of the reduced background expression from the chromosome. Vector copy number may be amplified. Cell cycles may be synchronized.
    Type: Grant
    Filed: December 18, 2006
    Date of Patent: September 7, 2010
    Assignee: Cambridge Microbial Technologies Limited
    Inventors: David Keith Summers, Ellie Chant