Pseudomonas Patents (Class 435/253.3)
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Patent number: 10674731Abstract: The present invention provides a package (200) comprising at least one first component in the form a well 220, the first component comprising particulate matter comprising at least one natural oil; at least one second component, in the form of a plurality of second wells 230 comprising antagonist(s) of a microbial pathogen; wherein the at least one first component and the at least one second component are contained in separate compartments of said package. Also provided by the present invention is a method for providing an anti-bacterial agent, the method comprising mixing the first component comprising particulate matter carrying at least one natural oil; and at least one second component comprising at least one antagonist of a microbial pathogen, and allowing said mixture to form into an emulsion with anti-bacterial activity.Type: GrantFiled: June 20, 2016Date of Patent: June 9, 2020Assignee: Nobactra Israel Ltd.Inventor: Giora Kritzman
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Patent number: 10675326Abstract: The present invention discloses methods and materials for killing and/or inhibiting the growth of a cancer cell via preferential entry of a cytotoxic compound. Preferential entry of the cytotoxic compound is accomplished by the use of protein transduction domains derived from cupredoxins, including the p18 and p28 truncations of azurin.Type: GrantFiled: December 11, 2017Date of Patent: June 9, 2020Inventors: Brad N. Taylor, Rajeshwari Mehta, Tohru Yamada, Craig Beattie, Tapas Das Gupta
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Patent number: 10525100Abstract: Provided are activating transcription factor 5 (ATF5) peptides having a truncated ATF5 leucine zipper region and, optionally, a cell-penetrating region, compositions comprising the ATF5 peptides, and methods of inhibiting proliferation of and promoting cytotoxicity in a neoplastic cell using the ATF5 peptides.Type: GrantFiled: January 3, 2019Date of Patent: January 7, 2020Assignee: Sapience Therapeutics, Inc.Inventors: Barry Jay Kappel, Gene Merutka, Jimmy Andrew Rotolo
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Patent number: 10526668Abstract: Provided are a Streptomyces (Streptomyces hygroscopicus) HS7522 and a method for preparing milbemycin A4 by culturing the Streptomyces. The Streptomyces (Streptomyces hygroscopicus) HS7522 of the present invention is deposited in “China General Microbiological Culture Collection Center” with an accession number of CGMCC No. 9671 on Sep. 16, 2014.Type: GrantFiled: March 25, 2016Date of Patent: January 7, 2020Assignee: Zhejiang Hisun Pharmaceutical Co., Ltd.Inventors: Yun Teng, Meidong Xu, Meiyi Mo, Zhihui He, Zhengjie Chen, Lianiqing Jiang, Hua Bai
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Patent number: 10369176Abstract: Provided herein are compositions comprising a biocompatible microsphere, a biofilm-generating probiotic bacterium, a prebiotic, and/or a prebiofilmic. Methods for preparing and formulating the compositions and methods for treating or preventing a disease using the compositions are also provided.Type: GrantFiled: September 6, 2016Date of Patent: August 6, 2019Assignees: Research Institute at Nationwide Children's Hospital, Ohio State Innovation FoundationInventors: Steven D. Goodman, Lauren O. Bakaletz, Gail Besner, Michael Bailey
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Patent number: 10363298Abstract: The present invention is based on the finding that in addition to interfering with or blocking, preventing and/or inhibiting the interaction between a pathogen and, for example, a sialic acid containing cell surface receptor, certain sialic acid binding molecules have immunomodulatory properties. The invention provides methods and uses which exploit sialic acid binding molecules in the treatment and/or prevention of disease by modulation and/or priming of the host immune response.Type: GrantFiled: January 23, 2015Date of Patent: July 30, 2019Assignee: University Court of the University of St. AndrewsInventors: Garry Taylor, Helen Connaris
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Patent number: 10336790Abstract: Disclosed herein are novel anti-microbial peptides with inhibitory activity against M. tuberculosis and streptococcus bacteria. Additionally, a method for designing novel anti-microbial peptides is disclosed.Type: GrantFiled: September 8, 2016Date of Patent: July 2, 2019Assignee: Rensselaer Polytechnic InstituteInventors: Georges Belfort, C. Seth Pearson, Brian Murray, Pankaj Sakharam Karande, Jun Ha Kwak, Kathleen A. McDonough, Zachary Andrew Kloos
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Patent number: 9701673Abstract: A species of Burkholderia sp with no known pathogenicity to vertebrates but with pesticidal activity (e.g., plants, insects, fungi, weeds and nematodes) is provided. Also provided are natural products derived from a culture of said species and methods of controlling pests using said natural products.Type: GrantFiled: February 24, 2011Date of Patent: July 11, 2017Assignee: Marrone Bio Innovations, Inc.Inventors: Ratnakar Asolkar, Marja Koivunen, Pamela Marrone, Huazhang Huang, Ana-Lucia Cordova-Kreylos
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Patent number: 9688726Abstract: The present invention provides a polypeptide that is rich in leucine and used for preventing and restraining inflammation, and an application of same. The present invention further provides a method for preparing the polypeptide and a pharmaceutical composition containing the polypeptide. The advantage of the polypeptide comprises: small molecular weight, so as to permeate various eye tissue barriers; high water solubility, so as to have high dissolubility in neutral tears, aqueous humor and vitreous humor; and simple synthesis, so as to have a low preparation cost.Type: GrantFiled: December 19, 2011Date of Patent: June 27, 2017Assignees: Shanghai First People's HospitalInventors: Xun Xu, Yan Xu
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Patent number: 9562073Abstract: The invention relates generally to polypeptides that include a cleavable moiety that is a substrate for at least one protease selected from matriptase and u-plasminogen activator (uPA), to activatable antibodies and other larger molecules that include the cleavable moiety that is a substrate for at least one protease selected from matriptase and u-plasminogen activator, and to methods of making and using these polypeptides that include a cleavable moiety that is a substrate for at least one protease selected from matriptase and u-plasminogen activator in a variety of therapeutic, diagnostic and prophylactic indications.Type: GrantFiled: January 30, 2015Date of Patent: February 7, 2017Assignee: CYTOMX THERAPEUTICS, INC.Inventors: Stephen James Moore, Margaret Thy Luu Nguyen, Daniel R. Hostetter, Olga Vasiljeva
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Patent number: 9409829Abstract: The invention provides an inoculant comprising Pseudomonas diazotrophicus and a carrier, which is useful for enhancing plant growth. The invention also provides a method of producing a plant which comprises applying an inoculant comprising Pseudomonas diazotrophicus and a carrier to plant or the growth medium of the plant.Type: GrantFiled: March 14, 2014Date of Patent: August 9, 2016Assignee: TEKS Inc.Inventor: Taylor Strehl
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Patent number: 9378492Abstract: A system, method, and computer program product are provided for automatically resolving non-sufficient funds (NSF) errors in a network. In use, it is automatically detected that a non-sufficient funds (NSF) error has occurred in a network, the NSF error being associated with a transaction of a consumer of the network while attempting to charge a first account. Additionally, it is automatically determined that the consumer has designated at least one alternative second account. Further, it is automatically determined that at least one of the at least one alternative second account has sufficient funds to complete the transaction. Furthermore, the at least one of the at least one alternative second account is automatically charged to complete the transaction.Type: GrantFiled: January 31, 2014Date of Patent: June 28, 2016Assignee: Amdocs Software Systems LimitedInventor: Mitchell Gordon Cole
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Patent number: 9029121Abstract: A method for the biological decomposition of organic compounds of the group of hydrocarbons, fats, oils, waxes, and derivatives thereof as well as mixtures thereof in media polluted therewith, in the presence of at least one dispersant, wherein the medium polluted with the pollutants is treated with at least one dispersant and with microorganisms.Type: GrantFiled: June 15, 2010Date of Patent: May 12, 2015Assignee: Total Marketing ServicesInventors: Ursula Fritzmeier, Robert Mallee, Achim Wiessler
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Patent number: 9012200Abstract: The object of the present invention is a composition of bacterial strains which may comprise Stenotrophomonas sp. strain 2L, Stenotrophomonas sp. strain 5L, Stenotrophomonas sp. strain 6L, Stenotrophomonas sp. strain 3N, Achromobacter sp. strain 4P, Arthrobacter sp. strain 1N, Brevundimonas sp. strain 2N, Brevundimonas sp. strain 5N, Brevundimonas sp. strain 6N, Pseudomonas sp. strain 3G, and Pseudomonas sp. strain 4, deposited under the number KKP 2041p (IAFB Collection of Industrial Microorganisms—Institute of Agricultural and Food Biotechnology in Warsaw), a bioremediation vaccine (bioremediation mixture) which may comprise the composition of these strains, the use of the vaccine in the removal of contaminants from the soil, and the method for the treatment of contaminated soil.Type: GrantFiled: January 24, 2014Date of Patent: April 21, 2015Assignee: Uniwersytet WarszawskiInventors: Magdalena Popowska, Hanka Boszczyk-Maleszak, Iga Komorowska
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Publication number: 20150104834Abstract: A specialized culture medium for the promotion of alginate production by stable mucoid Pseudomonas aeruginosa bacterial strains and methods for the production and purification of industrial, commercial, and pharmaceutical grade alginate from bacteriological sources are provided herein. Alginate produced using the media and methods disclosed herein is structurally uniform and substantially free of bacterial cell contaminants, including endotoxin.Type: ApplicationFiled: October 16, 2014Publication date: April 16, 2015Inventors: Hongwei David Yu, Richard M. Niles, Xin Wang, Kristy Dawn Dillon
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Publication number: 20150079661Abstract: The present invention is a bacteria and nutrient delivery composition containing bran. The subject composition is preferably made in the form of a tablet that is structurally stable without being excessively hard. The tablets preferably have a configuration that reduces the likelihood of premature shearing in tableting presses or jamming in feeder devices for biomass generators. Methods of manufacturing the bacterial delivery composition in a structurally stable form that maintains bacterial viability are also provided.Type: ApplicationFiled: April 25, 2014Publication date: March 19, 2015Applicant: NCH CorporationInventors: Judith G. Pruitt, Robert Clarence Pearce, III, John Ray Roheim
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Publication number: 20150050718Abstract: The problem is to provide a method for inexpensively and simply preserving an enzyme of a microbial biomass obtained by culturing and improve the enzyme activity during preservation. Provided is a method for preserving nitrile hydratase, characterized in that microbes having nitrile hydratase activity are cultured while being protected from light, and the resulting biomass is crushed using a high-pressure homogenizer and preserved while being protected from light.Type: ApplicationFiled: February 19, 2013Publication date: February 19, 2015Applicant: MITSUBISHI RAYON CO., LTD.Inventors: Emi Ishii, Masahito Oda
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Patent number: 8921068Abstract: A method and point of use kit to determine under field conditions the presence and/or amount of a selected Pseudomonas species capable of bioremediating organic environmental pollutants, such as petroleum hydrocarbons.Type: GrantFiled: April 24, 2013Date of Patent: December 30, 2014Assignee: CL Solutions, LLCInventors: Michael T. Saul, Sergey Gazenko
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Publication number: 20140377844Abstract: A method of deactivating an explosive composition being used in a blasting operation, which method comprises exposing the explosive composition to a micro-organism that is indigenous to the environment in which the explosive composition is being used and that is capable of producing an enzyme that degrades the explosive composition, wherein the explosive composition has associated with it a chemical inducing agent that promotes production of the enzyme by the micro-organism.Type: ApplicationFiled: January 29, 2013Publication date: December 25, 2014Inventors: Clint Brearley, Steven Kotsonis, Kaiyan Liu, Thomas Smylie, Richard Goodridge
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Publication number: 20140378646Abstract: The present application is directed at microorganisms of the genus Pseudomonas as deposited under DSM26199 (Pseudomonas sp. IPB-B26) and DSM26200 (Pseudomonas sp, N-128) with the Leibnitz Institute DSMZ. The present application is further directed at a process for the production of medium- and long-chain-length PHAs, comprising cultivating said microorganisms in a culture medium comprising a carbon source and isolating the PHA from the microorganisms. It has been observed that these microorganisms allow for efficient PHA production in high yields. In addition, the inventive microorganisms possess the valuable capability to incorporate unsaturated fatty acids into the resulting PHAs. The inventive microorganisms thus enable a later modification of the PHAs as well as cross-linking, thus opening new fields of applications for these materials.Type: ApplicationFiled: June 25, 2014Publication date: December 25, 2014Inventors: Monica Bassas Galia, Sagrario Arias Rivas, Gabriella Molinari, Kenneth Nigel Timmis
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Publication number: 20140377239Abstract: A biological material is disclosed for exerting antagonism against the mushroom pathogen Pseudomonas bacteria, which contains the Pseudomonas synxantha PS54bacterium strain deposited under # NCAIM (P) B 001387 according to the Budapest treaty. The biological material according to the invention is an effective antagonist against the pathogens of mushrooms, in particular oyster mushrooms and/or champignons, in particular Pseudomonas tolaasii and Pseudomonas agarici. Furthermore, the invention relates to a composition comprising a culture of the biological material according to the invention, the use of the invention for the protection of mushrooms, and a process for controlling pathogens. The invention further relates to an agent for producing mushrooms, which agent has been contacted with the biological material according to the invention.Type: ApplicationFiled: August 30, 2012Publication date: December 25, 2014Inventors: László Manczinger, Csaba Vágvölgyi, Enikö Sajben, Arpád Nagy, Zoltán Szöke-Kis, Adrienn Nagy, György Turóczi, András Kovács
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Publication number: 20140371194Abstract: This invention relates generally to the discovery of a method of inhibiting, preventing or treating bacterial infections. The invention also relates to a method of inhibiting bacterial capsule biogenesis.Type: ApplicationFiled: June 16, 2014Publication date: December 18, 2014Inventors: Patrick C. Seed, Carlos C. Goller, Apurba Dutta, Brooks Maki, Frank Schoenen, James Noah, Lucile White
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Patent number: 8871491Abstract: The present invention encompasses a novel S. aureus bioconjugate vaccine. More generally, the invention is directed to Gram-positive and other bioconjugate vaccines containing a protein carrier, at least one polysaccharide such as a capsular Gram-positive polysaccharide, and, optionally, an adjuvant or pharmaceutically acceptable carrier. The instant invention also includes methods of producing Gram-positive and other bioconjugate vaccines. An N-glycosylated protein is also provided that contains one or more polysaccharides such as Gram-positive polysaccharides. The invention is additionally directed to engineered prokaryotic organisms comprising nucleotide sequences encoding a glycosyltransferase of a first prokaryotic organism and a glycosyltransferase of a second prokaryotic organism. The invention further includes plasmids and prokaryotic cells transformed with plasmids encoding polysaccharides and enzymes which produce an N-glycosylated protein and/or bioconjugate vaccine.Type: GrantFiled: May 4, 2011Date of Patent: October 28, 2014Assignee: Glycovaxyn AGInventors: Michael Wacker, Michael Kowarik, Michael Wetter
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Publication number: 20140315310Abstract: The invention provides, inter alia, recombinase-based systems that provide for integrated logic and memory in living cells.Type: ApplicationFiled: December 13, 2013Publication date: October 23, 2014Applicant: Massachusetts Institute of TechnologyInventors: Timothy Kuan-Ta Lu, Piro Siuti
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Publication number: 20140256021Abstract: A biofilter media has one or more of a set of desired physical characteristics. The set of physical characteristics includes a sphericity of 0.75 to 1, a particle size of 1 to 16 or 4 to 8 mm, a uniformity coefficient of 2 or less and a surface area of 800 to 2000 m2/m3of media. The media is coated, supports microorganisms and is used to treat a waste gas stream.Type: ApplicationFiled: May 21, 2014Publication date: September 11, 2014Applicant: BIOREM TECHNOLOGIES INCInventors: Hidayat HUSAIN, Brian P. HERNER
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Patent number: 8822662Abstract: The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerina and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: GrantFiled: December 8, 2009Date of Patent: September 2, 2014Assignee: BASF Plant Science Company GmbHInventors: Toralf Senger, Jörg Bauer, Johnathan A. Napier
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Patent number: 8796179Abstract: The invention describes an isolate of a fluorescent pseudomonad of the species Pseudomonas azotoformans, strain F30A, which has been deposited at Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH and has been assigned accession number DSM 22077, which is capable of enhancing seed germination, seedling establishment, plant emergence, plant growth and/or the yields of crops treated with the isolate. Accordingly the invention further comprises the use of this pseudomonad for enhancement of plant emergence and growth and agricultural compositions comprising the pseudomonad.Type: GrantFiled: December 22, 2010Date of Patent: August 5, 2014Assignee: Lantmannen Bioagri ABInventors: Jolanta Levenfors, Christopher Folkeson Welch, Jamshid Fatehi, Mariann Wikstrom, Sara Rasmussen, Margareta Hökeberg
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Publication number: 20140206539Abstract: This disclosure presents the Pseudomonas fluorescens strain GM30, compositions containing the GM30 strain, and methods of using the GM30 strain to enhance plant growth and/or enhance plant resistance to pathogens.Type: ApplicationFiled: January 23, 2013Publication date: July 24, 2014Applicant: UT-BATTELLE, LLCInventors: Mitchel John DOKTYCZ, Dale A. PELLETIER, Christopher Warren SCHADT, Gerald A. TUSKAN, David WESTON
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Patent number: 8765404Abstract: Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production.Type: GrantFiled: March 2, 2011Date of Patent: July 1, 2014Assignee: Massachusetts Institute of TechnologyInventors: Gregory Stephanopoulos, Syed Hussain Imam Abidi
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Patent number: 8735136Abstract: Substantially pure biological culture of a strain of the species Pseudomonas graminis, deposited with number CBS136973 at the depositary institution “Centraalbureau voor Schimmelcultures” (CBS) in Utrecht, Netherlands. Use of the CBS136973 culture as an antagonist for the biocontrol of foodborne pathogenic bacteria in fruit intended for human consumption. Method for treating the fruit which comprises the step of applying a preparation that comprises a culture of a strain of the species Pseudomonas graminis, deposited with number CBS136973 at the depositary institution “Centraalbureau voor Schimmelcultures” (CBS) in Utrecht, Netherlands, to the fruit. The application thereof makes it possible to reduce the growth of pathogens during the shelf life of the product, especially when the cold chain is broken.Type: GrantFiled: December 29, 2011Date of Patent: May 27, 2014Assignees: Institut de Recerca I Tecnologia Agroalimentaries, Universitat de LleidaInventors: Immaculada Vinas Almenar, Maria Isabel Abadias Sero, Josep Usall Rodie, Neus Teixido Espasa, Rosario Torres Sanchis
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Patent number: 8720546Abstract: Disclosed herein is introduction of different nutrient solution formulations to a subterranean target site to prevent biofilm formation and biomass aggregation at the sand face of an injection well bore during MEOR or bioremediation processes. A first nutrient solution formulation supports growth of introduced microorganisms and a second nutrient solution formulation is used to promote biofilm formation to allow plugging of pores and channels in the subterranean target site, for MEOR or bioremediation.Type: GrantFiled: October 25, 2011Date of Patent: May 13, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Albert W. Alsop, Sheryl M. Anyzek, Robert D. Fallon, Edwin R. Hendrickson, Sharon Jo Keeler, Abigail K. Luckring, Michael P. Perry
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Publication number: 20140128364Abstract: Disclosed herein is the use of tigemonam and carumonam in treating bacterial infection caused by bacteria producing K. pneumoniae carbapenemase (KPC) enzymes.Type: ApplicationFiled: March 12, 2013Publication date: May 8, 2014Applicant: Rempex PharmaceuticalsInventor: Olga Rodny
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Publication number: 20140109249Abstract: The present invention relates to methods for the screening, identification and/or application of microorganisms of use in imparting beneficial properties to plants.Type: ApplicationFiled: October 10, 2013Publication date: April 17, 2014Applicant: Biodiscovery New Zealand LimitedInventors: Susan Jane Turner, Peter John Wigley
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Publication number: 20140057272Abstract: A method of culturing fastidious bacteria where a conditioned cell culture medium is prepared in which eukaryotic cells have been cultured, but where the medium is substantially free of the cultured cells. Fastidious bacteria are cultured in the medium. The conditioned cell culture medium can contain secreted factors from the cells. These factors promote the growth of the fastidious bacteria. The fastidious bacterial culture is maintained for a time period sufficient for the fastidious bacteria to multiply. The fastidious bacteria are analyzed using PCR, DNA sequencing or microbiology characterization.Type: ApplicationFiled: August 21, 2013Publication date: February 27, 2014Applicant: MRIGlobalInventors: Kelly Brown, Richard Winegar, Jacqueline Bortzner, Nicole Moritz, Stephanie Sorrell
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Publication number: 20140038266Abstract: The invention relates to a method of preparing an oil absorbent composition. The method comprises heating and then de-mineralising a precursor plant material under conditions suitable to produce an oil absorbent composition comprising charcoal. The invention extends to oil absorbent compositions per se, such as charcoal-based compositions, and to various uses of the compositions for efficiently and rapidly absorbing spilled oil, for example from water surfaces, or from bituminous sands.Type: ApplicationFiled: October 3, 2013Publication date: February 6, 2014Applicant: The Forestry CommissionInventors: Franciscus Antonius Anna Maria De Leij, James Stratford, Tony Hutchings
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Publication number: 20140017767Abstract: The present invention relates to microbial inocula and in particular to standardised inocula for producing reference cultures of microorganisms. The invention has been developed primarily for use as a solid, disc-shaped, inoculum and will be described hereinafter with reference to this application. However, it will be appreciated that the invention is not limited to this particular field of use.Type: ApplicationFiled: June 21, 2013Publication date: January 16, 2014Applicant: AMS Laboratories Pty LtdInventors: Paul PRISCOTT, Yi-hong WANG
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Patent number: 8623390Abstract: Six bacterial strains: Bacillus simplex strain 03WN13, Bacillus simplex strain 03WN23, Bacillus simplex strain 03WN25, Pseudomonas koreensis strain 10IL21, Pantoea agglomerans strain 10IL31, and Pseudomonas lini strain 13IL01, are superior antagonists of Phytophthora erythroseptica Pethybr., the causative agent of pink rot on potatoes. These bacterial strains are effective for suppression and control of pink rot on potatoes.Type: GrantFiled: September 24, 2010Date of Patent: January 7, 2014Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: David A. Schisler, Patricia J. Slininger, Tugba Adiyaman
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Patent number: 8591926Abstract: The present invention provides formulations and methods for preventing, suppressing, treating, or controlling pre- or post-harvest disease or decay in plants. In the inventive method, plants are contacted with a formulation including an antagonistic microorganism and a booster composition. The booster composition generally includes about 3 parts Kaolin clay, about 1 part yeast, about 1 part Yucca plant extract, and about 1 part calcium-source material. The antagonistic microorganism may be included in an amount of between about 0.02 parts and about 0.5 parts by weight of the formulation, with about 0.04 parts antagonistic microorganism being preferred in testing to date. The formulation is typically applied to the above ground structures of the plant, including its leaves, flowers, stems, trunk, blossoms and fruit.Type: GrantFiled: March 15, 2011Date of Patent: November 26, 2013Inventor: William Brower
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Publication number: 20130288340Abstract: An object of the present invention is to provide a microorganism which is novel at the elemental composition level, and to provide a technique for providing such a microorganism. The present inventor has succeeded in causing a microorganism to efficiently contain a non-essential element by decreasing the content of an essential nutrient source for the microorganism, a C source, an N source, a P source, or an S source, and by adding an X compound containing the non-essential element as a constitutive element in a manner to make up for the decreased amount, and then culturing the microorganism.Type: ApplicationFiled: September 5, 2011Publication date: October 31, 2013Applicants: PARA MICROBIOS LABORATORIES LLCInventor: Yoshitake Tanaka
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Publication number: 20130288323Abstract: A process for producing medium-chain-length 3-hydroxyalkanoic acids in a liquid medium, the process comprising: (i) intermixing a liquid culture medium and a microbial inoculum comprising a pure culture of a Pseudomonas putida strain having a portion of a phaC1 gene deleted and/or a portion of a phaC2 gene deleted and/or a portion of a phaZ gene deleted, (ii) culturing the mixture for a period of time to allow for microbial cell growth to occur in the liquid culture medium thereby producing a spent liquid medium and a plurality of microbial cells wherein medium-chain-length 3-hydroxyalkanoic acids are synthesized, (iii) separating the medium-chain-length 3-hydroxyalkanoic acids from the plurality of microbial cells and the spent liquid medium; and (iv) recovering the separated medium-chain-length 3-hydroxyalkanoic acids. A pure culture comprising transgenic microbial cells wherein a portion of a phaC1 gene and/or a phaC2 gene and/or a phaZ gene has been deleted.Type: ApplicationFiled: November 29, 2011Publication date: October 31, 2013Applicant: UNIVERSITY OF MANITOBAInventors: David B. Levin, Parveen Sharma
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Publication number: 20130280226Abstract: Substantially pure biological culture of a strain of the species Pseudomonas graminis, deposited with number CBS124167 at the depositary institution “Centraalbureau voor Schimmelcultures” (CBS) in Utrecht, Netherlands. Use of the CBS124167 culture as an antagonist for the biocontrol of foodborne pathogenic bacteria in fruit intended for human consumption. Method for treating the fruit which comprises the step of applying a preparation that comprises a culture of a strain of the species Pseudomonas graminis, deposited with number CBS124167 at the depositary institution “Centraalbureau voor Schimmelcultures” (CBS) in Utrecht, Netherlands, to the fruit. The application thereof makes it possible to reduce the growth of pathogens during the shelf life of the product, especially when the cold chain is broken.Type: ApplicationFiled: December 29, 2011Publication date: October 24, 2013Applicants: INSTITUT RECERCA I TECNOLOGIA AGROALIMENTARIES, UNIVERSITAT DE LLEIDAInventors: Immaculada Vinas Almenar, Maria Isabel Abadias Sero, Josep Usall Rodie, Neus Teixido Espasa, Rosario Torres Sanchis
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Patent number: 8524481Abstract: Provided in part herein are compositions that include isolated microbial enzymes, such as proteases, lipases, useful in dehairing leather. Also provided are methods for isolating proteases, lipases, from microbes, as well as methods of reusing these enzymes effectively for several cycles in an environment friendly process.Type: GrantFiled: November 16, 2010Date of Patent: September 3, 2013Assignee: West Bengal University of TechnologyInventor: Shaon Ray Chaudhuri
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Patent number: 8507230Abstract: A method for producing polyhydroxyalkanoate (PHA), comprising (i) culturing in a culture medium comprising terephthalic acid and/or a salt thereof and/or an ester thereof one or more bacterial strains which are capable of accumulating PHA from terephthalic acid or a salt or ester thereof and which are selected from Pseudomonas putida strain GO16 having the accession number NCIMB 41538, Pseudomonas putida strain GO19 having the accession number NCIMB 41537, and Pseudomonas frederiksbergensis strain GO23 having the accession number NCIMB 41539; and (ii) recovering the PHA produced from the culture medium. The invention also provides Pseudomonas putida strain GO16 having the accession number NCIMB 41538; Pseudomonas putida strain GO19 having the accession number NCIMB 41537; and Pseudomonas frederiksbergensis strain GO23 having the accession number NCIMB 41539.Type: GrantFiled: April 6, 2009Date of Patent: August 13, 2013Assignee: University College Dublin, National University of IrelandInventors: Kevin O'Connor, Shane Kenny, Jasmina Nikodinovic
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Publication number: 20130196420Abstract: The present invention relates to compositions and methods for the bioremediation of hydrocarbon-containing waste, such as drill mud and drill cuttings from oil and gas wellbores, drill waste deposit sites, oil spills, oil and gas production waste, and contaminated surfaces, using wood chips comprising microorganisms of mountain pine beetle.Type: ApplicationFiled: February 27, 2013Publication date: August 1, 2013Inventor: Mark Joseph Mathis
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Patent number: 8450098Abstract: The method disclosed herein, relates generally to introducing molecules such as biomolecules (e.g., nucleic acids) into a filamentous fungus. More specifically, the methods disclosed herein relate to introducing one or more nucleic acids into a filamentous fungus.Type: GrantFiled: May 21, 2008Date of Patent: May 28, 2013Assignee: Danisco US Inc.Inventors: Steven Kim, Andrei Miasnikov
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Publication number: 20130035232Abstract: Provided herein are methods for inducing and stabilizing an enzyme activity. Optionally, the enzyme is in a microorganism capable of producing the enzyme. In some embodiments, the enzyme can be nitrile hydratase, amidase, or asparaginase I. Provided are compositions comprising enzymes or microorganisms having induced and/or stabilized activity. Also provided are methods of delaying a plant development process by exposing a plant or plant part to the enzymes or microorganisms having induced and/or stabilized activity.Type: ApplicationFiled: January 24, 2011Publication date: February 7, 2013Applicant: GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC.Inventors: George E. Pierce, Trudy Ann Tucker
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Patent number: 8357526Abstract: The present disclosure relates to isolation and identification of unique Pseudomonas stutzeri strains that can grow on crude oil under denitrifying conditions and are useful in oil recovery.Type: GrantFiled: April 18, 2008Date of Patent: January 22, 2013Assignee: E.I. du Pont de Nemours and CompanyInventors: Sharon Jo Keeler, Robert D. Fallon, Edwin R. Hendrickson, Linda L. Hnatow, Scott Christopher Jackson, Michael P. Perry
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Patent number: 8354265Abstract: The present invention concerns the field of bacterial biocontrol. More precisely, the invention relates to the identification of chemicals which promote the growth of bacteria inactivating NAHL, such as gamma-caprolactone (GCL) and 4-heptanolide (HTN) and their use in soil additives.Type: GrantFiled: January 18, 2008Date of Patent: January 15, 2013Assignees: Centre National de la Recherche Scientifique, Comite Economique Agricole Production des Plants du Nord (Comite Nord Pomme de Terre)Inventors: Denis Faure, Amélie Cirou, Yves Dessaux
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Publication number: 20130005572Abstract: The invention describes an isolate of a fluorescent pseudomonad of the species Pseudomonas azotoformans, strain F30A, which has been deposited at Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH and has been assigned accession number DSM 22077, which is capable of enhancing seed germination, seedling establishment, plant emergence, plant growth and/or the yields of crops treated with the isolate. Accordingly the invention further comprises the use of this pseudomonad for enhancement of plant emergence and growth and agricultural compositions comprising the pseudomonad.Type: ApplicationFiled: December 22, 2010Publication date: January 3, 2013Inventors: Jolanta Levenfors, Christopher Folkeson Welch, Jamshid Fatehi, Mariann Wikstrom, Sara Rasmussen, Margareta Hökeberg
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Publication number: 20120329138Abstract: A process for separation of a mixture containing a microbial substance and a liquid using deformable filter is provided.Type: ApplicationFiled: December 19, 2011Publication date: December 27, 2012Applicant: SHELL OIL COMPANYInventors: Henry Van Kaathoven, Johannes Pieter Haan, Wilhelmus Maria Bond, Johannes Leendert Willem Cornelis Den Boestert