Aspergillus Patents (Class 435/254.3)
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Publication number: 20140147893Abstract: The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: July 6, 2012Publication date: May 29, 2014Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Aki Tomiki, Guillermo Coward-Kelly
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Publication number: 20140147458Abstract: The present invention relates to novel Varicella-zoster virus strain and to a chicken pox and herpes zoster virus vaccine using the same. More particularly, the present invention relates to genome DNA of VZV MAV/06 strain isolated from a Korean patient, to an open reading frame (ORF) thereof, to a protein coded by the genome DNA of VZV MAV/06 strain and the ORF thereof, and to a vaccine composition which contains the protein as an active ingredient.Type: ApplicationFiled: February 24, 2012Publication date: May 29, 2014Applicant: MOGAM BIOTECHNOLOGY RESEARCH INSTITUTEInventors: Geun Hee Kim, Shi Nae Kwon, Chan Hee Lee, Yeop Yoon
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Publication number: 20140147894Abstract: The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.Type: ApplicationFiled: October 6, 2011Publication date: May 29, 2014Applicant: BP Corporation North America Inc.Inventors: Justin T. Stege, Alexander Varvak, John Poland, Chris S. Lyon, Shaun Healey, Peter Luginhuhl
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Patent number: 8735563Abstract: The present invention relates to isolated polynucleotides having promoter activity the juse of the isolated polynucleotides for the procuction of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: GrantFiled: June 23, 2011Date of Patent: May 27, 2014Assignee: Novozymes A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa
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Publication number: 20140140999Abstract: The invention provides means and methods for producing one or more Ig-like molecules in a single host cell. Novel CH3 mutations enabling the production of monospecific and/or bispecific Ig-like molecules of interest are also provided.Type: ApplicationFiled: November 15, 2013Publication date: May 22, 2014Applicant: MERUS B.V.Inventors: Cornelis A. DE KRUIF, Linda Johanna Aleida HENDRIKS, Ton LOGTENBERG
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Publication number: 20140141467Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.Type: ApplicationFiled: January 23, 2014Publication date: May 22, 2014Applicant: Biocon LimitedInventors: Sanjay Tiwari, Mukesh Babuappa Patale, Saurabh Garg, Mayank Kumar Garg, Sulekha Joshi, Chittnalli Ramegowda Naveen Kumar, Bimal Kumar, Anuj Goel, Harish Iyer
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Publication number: 20140141012Abstract: The invention relates to binding compounds that specifically bind to human TSLP, as well as uses thereof, e.g., in the treatment of inflammatory disorders and allergic inflammatory response.Type: ApplicationFiled: January 28, 2014Publication date: May 22, 2014Applicant: MERCK SHARP & DOHME CORP.Inventor: Leonard G. Presta
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Publication number: 20140141482Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.Type: ApplicationFiled: June 12, 2013Publication date: May 22, 2014Applicant: INVISTA North America S.á r.l.Inventors: Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140134706Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: ApplicationFiled: October 29, 2013Publication date: May 15, 2014Applicant: AB Enzymes OyInventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo
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Publication number: 20140134736Abstract: The present disclosure relates to methods of controlling the sulfide (S2˜) content in systems, such as oil and gas reservoirs and pipelines, by the use of chlorine oxyanions and microorganisms with (per)chlorate-reducing activity.Type: ApplicationFiled: May 31, 2012Publication date: May 15, 2014Applicant: The Regents of the University of CaliforniaInventor: John D. Coates
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Publication number: 20140134707Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderma. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: ApplicationFiled: October 30, 2013Publication date: May 15, 2014Applicant: AB Enzymes OyInventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo, George Szakacs
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Publication number: 20140134681Abstract: The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam.Type: ApplicationFiled: December 13, 2013Publication date: May 15, 2014Inventors: Petronella Catharina RAEMAKERS-FRANKEN, Martin SCHURMANN, Axel Christoph TREFZER, Stefaan Marie Andre DE WILDEMAN
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Publication number: 20140134688Abstract: The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: ApplicationFiled: January 21, 2014Publication date: May 15, 2014Applicant: Novozymes, Inc.Inventors: Amanda Fischer, Stephen Brown, Sheryl Luttringer
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Patent number: 8722382Abstract: A xylanase having increased enzymaic activity is disclosed. The xylanase has a modified amino acid sequence of SEQ ID NO: 2, wherein Tryptophan at position 125 is substituted with Phenylalanine and Phenylalanine at position 163 is substituted with Tryptophan.Type: GrantFiled: September 27, 2013Date of Patent: May 13, 2014Assignee: Dongguan APAC Biotechnology Co., Ltd.Inventors: Rey-Ting Guo, Ya-Shan Cheng, Jian-Wen Huang, Tzu-Hui Wu, Hui-Lin Lai, Cheng-Yen Lin, Ting-Yung Huang
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Patent number: 8722387Abstract: The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: GrantFiled: February 28, 2012Date of Patent: May 13, 2014Assignee: Novozymes, Inc.Inventors: Amanda Fischer, Stephen Brown, Sheryl Luttringer
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Publication number: 20140127754Abstract: Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.Type: ApplicationFiled: November 5, 2012Publication date: May 8, 2014Applicant: DYADIC INTERNATIONAL, INC.Inventors: Marco A. Baez-Vasquez, Richard Burlingame, Jon K. Magnuson, Marion Bradford, Arkady Panteleimonovich Sinitsyn
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Publication number: 20140127759Abstract: A ?-glucosidase exhibits high activity in the presence of biomass and has high thermal stability compared to conventional enzymes. The ?-glucosidase includes substitutions and/or deletions of amino acids, at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent ?-glucosidase with other amino acids and exhibits a decomposition activity.Type: ApplicationFiled: January 14, 2014Publication date: May 8, 2014Applicant: Toray Industries, Inc.Inventors: Hiroyuki Kurihara, Katsushige Yamada, Kazuhiko Ishikawa, Yumiko Mishima, Yasunobu Wada, Yuji Kado
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Patent number: 8716023Abstract: The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expType: GrantFiled: December 9, 2004Date of Patent: May 6, 2014Assignees: Novozymes, Inc., Novozymes A/SInventors: Howard Brody, Suchindra Maiyuran, Hiroaki Udagawa
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Publication number: 20140121153Abstract: The present invention relates to an isolated polypeptide comprising (a) the amino acid sequence set forth in SEQ ID NO: 21; or (b) an amino acid sequence that is at least 95% identical to SEQ ID NO: 21, and to therapeutic treatments based thereon.Type: ApplicationFiled: January 2, 2014Publication date: May 1, 2014Inventors: Tamara SANDLER, Orly DEVARY
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Patent number: 8710205Abstract: The present invention relates to a transcription factor found in filamentous fungi, especially in Aspergillii, DNA sequences coding for said factor, its transformation into and expression in fungal host organisms, and the use of said factor in such hosts for increasing the expression of a polypeptide of interest being produced by said host.Type: GrantFiled: February 20, 2003Date of Patent: April 29, 2014Assignee: Novozymes A/SInventor: Tove Christensen
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Patent number: 8709785Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.Type: GrantFiled: August 30, 2012Date of Patent: April 29, 2014Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Marguerite A. Cervin, Gopal K. Chotani, Frank J. Feher, Richard La Duca, Joseph C. McAuliffe, Andrei Miasnikov, Caroline M. Peres, Aaron S. Puhala, Karl J. Sanford, Fernando Valle, Gregory M. Whited
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Publication number: 20140113352Abstract: The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.Type: ApplicationFiled: December 19, 2013Publication date: April 24, 2014Applicant: Codexis, Inc.Inventors: Dipnath Baidyaroy, Louis Clark, Lisa M. Newman
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Publication number: 20140112914Abstract: By replacing the antigen-binding domain, the present inventors discovered novel polypeptide complexes that retain BiTE's strong anti-tumor activity and excellent safety properties, as well as have long half-life in blood and can damage various different target cells.Type: ApplicationFiled: November 30, 2011Publication date: April 24, 2014Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHAInventors: Junichi Nezu, Takahiro Ishiguro, Atsushi Narita, Akihisa Sakamoto, Yumiko Kawai, Tomoyuki Igawa, Taichi Kuramochi
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Publication number: 20140115739Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 11, 2013Publication date: April 24, 2014Inventors: Suchindra Maiyuran, Randall Kramer, Paul Harris
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Publication number: 20140113336Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 1, 2012Publication date: April 24, 2014Inventor: Nikolaj Spodsberg
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Publication number: 20140115740Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 13, 2013Publication date: April 24, 2014Applicants: Novozymes, Inc., Novozymes A/SInventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Isak Jorgensen, Randall Kramer
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Publication number: 20140113966Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: October 22, 2012Publication date: April 24, 2014Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: ROBERT DICOSIMO, MARK SCOTT PAYNE, JOHN EDWARD GAVAGAN
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Publication number: 20140113304Abstract: The present invention relates to a bi-directional cytosine deaminase-encoding selection marker as well as methods for using said marker.Type: ApplicationFiled: May 23, 2012Publication date: April 24, 2014Applicant: Novozymes A/SInventor: Hiroaki Udagawa
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Publication number: 20140113338Abstract: The invention relates to an alpha-ketopimelic acid decarboxylase enzyme that is a homologue of SEQ ID NO:2, comprising at least one mutation selected from a group of substitutions listed in the specification, to a method for preparing 5-formyl valeric acid (hereinafter also referred to as ‘5-FVA’), to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’), to a method for preparing ?-caprolactam (hereinafter referred to as ‘caprolactam’) from 6-ACA, to a method for the preparation of adipic acid, to a method for preparing diaminohexane. The invention further relates to a host cell which may be used in a method according to the invention and to a polynucleotide encoding an alpha-ketopimelic acid decarboxylase enzyme.Type: ApplicationFiled: March 30, 2012Publication date: April 24, 2014Applicant: DSM IP ASSETS B.V.Inventors: Axel Christoph Trefzer, Stefanus Cornelis Hendrikus Josef Turk, Jan Metske Van Der Laan, Iise De Lange, Denise Ilse Jacobs
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Publication number: 20140105889Abstract: The present invention demonstrated that the modification of the Fc region of an antigen-binding molecule into an Fc region that does not form in a neutral pH range a heterotetramer complex containing two molecules of FcRn and an active Fc? receptor improved the pharmacokinetics of the antigen-binding molecule and reduced the immune response to the antigen-binding molecule. The present invention also revealed methods for producing antigen-binding molecules having the properties described above, and successfully demonstrated that pharmaceutical compositions containing as an active ingredient such an antigen-binding molecule or an antigen-binding molecule produced by a production method of the present invention have excellent features over conventional antigen-binding molecules in that when administered, they exhibit improved pharmacokinetics and reduced in vivo immune response.Type: ApplicationFiled: March 30, 2012Publication date: April 17, 2014Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHAInventors: Tomoyuki Igawa, Atsuhiko Maeda, Kenta Haraya, Yuki Iwayanagi, Tatsuhiko Tachibana, Futa Mimoto, Taichi Kuramochi, Hitoshi Katada, Shojiro Kadono
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Publication number: 20140106410Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 20, 2013Publication date: April 17, 2014Applicant: NOVOZYMES, INC.Inventor: Nikolaj Spodsberg
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Publication number: 20140106457Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.Type: ApplicationFiled: September 23, 2013Publication date: April 17, 2014Inventors: Steen Troels Jorgensen, Poul Erik Pedersen
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Publication number: 20140099678Abstract: Novel fungal endoglucanases Cel5 and Cel12 are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry, or in hydrolysis of lignocellulosic material for, e.g. bioethanol production.Type: ApplicationFiled: October 18, 2013Publication date: April 10, 2014Applicant: AB Enzymes OyInventors: Terhi PURANEN, Leena VALTAKARI, Marika ALAPURANEN, George SZAKACS, Pentti OJAPALO, Jari VEHMAANPERA
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Publication number: 20140100338Abstract: The invention relates to a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. According to said method, at least one polyacrylic acid ester is provided and incubated with at least one enzyme selected from enzymes (EC 3.1) acting on ester bindings, until the ester groups contained in the polyacrylic acid ester are partially or fully hydrolytically split, and optionally the modified polymer obtained thereby is isolated. The invention also relates to the enzymes used and mutants thereof, nucleic acids coding for the enzymes, vectors comprising the nucleic acids, micro-organisms comprising the vectors, and the use of the enzymes, the vectors or the micro-organisms for carrying out a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. The present application also relates to polymer reaction products that can be obtained by the method, and methods for producing esterases.Type: ApplicationFiled: December 9, 2013Publication date: April 10, 2014Applicant: BASF SEInventors: Bernhard Hauer, Hans Wolfgang Höffken, Cecilia Kvarnström Branneby, Helmut Schwab, Sabine Feichtenhofer, Marlene Buchebner, Brigitte Pohn
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Publication number: 20140101793Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 12, 2013Publication date: April 10, 2014Applicants: Novoyzmes A/S, Novozymes, Inc.Inventor: Marc Dominique Morant
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Publication number: 20140096287Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 14, 2013Publication date: April 3, 2014Inventors: Kristian Krogh, Paul Harris
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Publication number: 20140093939Abstract: Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived from Aspergillus awamori or Aspergillus niger. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65° C. (pH 5.0, 30 min.Type: ApplicationFiled: November 25, 2013Publication date: April 3, 2014Applicant: AMANO ENZYME INC.Inventors: Megumi NAKAGAWA, Naoki MATSUMOTO, Hitoshi AMANO, Shotaro YAMAGUCHI
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Publication number: 20140093919Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: July 31, 2012Publication date: April 3, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140093940Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: October 12, 2012Publication date: April 3, 2014Applicant: Novozymes, Inc.Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
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Publication number: 20140086943Abstract: The present invention relates to peptides, nucleic acids, and cells for use in the immunotherapy of cancer. The present invention furthermore relates to survivin-derived tumor-associated cytotoxic T cell (CTL) peptide epitopes, alone or in combination with other tumor-associated peptides that serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses. The present invention specifically relates to three novel peptide sequences and variants thereof derived from HLA class I and class II molecules of human tumor cells that can be used in vaccine compositions for eliciting anti-tumor immune responses.Type: ApplicationFiled: March 15, 2013Publication date: March 27, 2014Applicant: IMMATICS BIOTECHNOLOGIES GMBHInventors: Toni WEINSCHENK, Peter LEWANDROWSKI, Oliver SCHOOR
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Publication number: 20140087426Abstract: This application provides transcription activator-like effector nucleases (TALENs), polynucleotide sequences encoding the TALENs, expression cassettes for producing TALENs to target cleavage of nucleic acids, and methods of producing and using the TALENs.Type: ApplicationFiled: September 24, 2013Publication date: March 27, 2014Applicant: The Chinese University of Hong KongInventors: Yun LIU, Christopher H.K. Cheng
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Publication number: 20140087442Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: August 30, 2013Publication date: March 27, 2014Applicant: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
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Patent number: 8679801Abstract: The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporter that can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbors other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.Type: GrantFiled: February 16, 2009Date of Patent: March 25, 2014Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek TNOInventors: Peter Jan Punt, Maria Johanna Van Der Werf
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Patent number: 8679815Abstract: The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity.Type: GrantFiled: December 19, 2007Date of Patent: March 25, 2014Assignee: Danisco US Inc.Inventors: Toby M. Baldwin, Benjamin S. Bower, Nigel Dunn-Coleman, Suzanne E. Lantz, Michael J. Pepsin
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Publication number: 20140080178Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 9, 2012Publication date: March 20, 2014Inventors: Kirk Schnorr, Randall Kramer
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Patent number: 8673618Abstract: This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, ?-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.Type: GrantFiled: October 20, 2010Date of Patent: March 18, 2014Assignee: Dyadic International (USA), Inc.Inventors: Alexander V. Gusakov, Tatyana N. Salanovich, Alexey I. Antonov, Boris B. Ustinov, Oleg N. Okunev, Richard P. Burlingame, Mark A. Emalfarb, Marco A. Baez, Arkady P. Sinitsyn
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Patent number: 8673599Abstract: The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam.Type: GrantFiled: March 11, 2009Date of Patent: March 18, 2014Assignee: DSM IP Assets B.V.Inventors: Petronella Catharina Raemakers-Franken, Martin Schurmann, Axel Christoph Trefzer, Stefaan Marie Andre De Wildeman
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Publication number: 20140075600Abstract: The present invention relates to a valencene synthase, to a nucleic acid encoding such valencene synthase, to a host cell comprising said encoding nucleic acid sequence and to a method for preparing valencene, comprising converting farnesyl diphosphate to valencene in the presence of a valencene synthase according to the invention.Type: ApplicationFiled: December 15, 2010Publication date: March 13, 2014Applicant: Isobionics B.V.Inventors: Jihane Achkar, Theodorus Sonke, Martinus Julius Beekwilder, Hendrik Jan Bouwmeester, Hendrik Jan Bosch
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Publication number: 20140072979Abstract: A method of detecting and isolating cells that produce a secreted protein of interest (POI) that has an immunoglobulin CH3 domain and/or substituted CH3 domain, comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.Type: ApplicationFiled: November 14, 2013Publication date: March 13, 2014Applicant: Regeneron Pharmaceuticals, Inc.Inventors: JAMES P. FANDL, GANG CHEN, NEIL STAHL, GEORGE D. YANCOPOULOS, DIPALI DESHPANDE, DARYA BURAKOV, THOMAS ALDRICH, VISHAL KAMAT
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Publication number: 20140073017Abstract: The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having ?-glucosidase activity, methods for hydrolyzing biomass material, and methods for improving the stability and saccharification efficacy of a composition comprising such ?-glucosidase polypeptides and/or activity.Type: ApplicationFiled: March 16, 2012Publication date: March 13, 2014Applicant: DANISCO US INC.Inventors: Thijs Kaper, Igor Nikolaev, Suzanne E. Lantz, Meredith K. Fujdala, Megan Y. Hsi