Yeast Patents (Class 435/255.1)
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Patent number: 11472960Abstract: A solid multiphase polymer blend material comprising: (i) a polyphenolic substance having a molecular weight of at least 500 g/mol; and (ii) a polyester having a molecular weight of at least 500 g/mol; wherein at least a portion of the polyphenolic substance is covalently bonded directly or through a linking moiety to the polyester. Methods for producing the blend material are also described, e.g., homogeneously melt blending a mixture comprising components (i) and (ii) under conditions resulting in covalent attachment of at least a portion of the polyphenolic substance directly or through a linking moiety to the polyester. Methods for producing objects made of the blend material by melt extrusion are also described.Type: GrantFiled: January 29, 2021Date of Patent: October 18, 2022Assignees: UT-Battelle, LLC, University of Tennessee Research FoundationInventors: Anthony S. Bova, Amit K. Naskar
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Patent number: 11319521Abstract: The present invention relates to methods and systems of pitching yeast to fermentation reactors. More particularly, the present invention involves pitching yeast from one fermentation tank to at least one additional fermentation tank. Advantageously, yeast can be continuously pitched from fermentor to fermentor for as long as practically desirable.Type: GrantFiled: December 13, 2018Date of Patent: May 3, 2022Assignee: POET Research, Inc.Inventors: Neelakantam V. Narendranath, Stephen M. Lewis, Neil D. Anderson
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Patent number: 11306336Abstract: A process for production of galacto-oligosaccharides is provided. The process for production of galacto-oligosaccharides includes growing Sporobolomyces singularis in a fermentation medium, harvesting the said microbes from the fermentation medium, inoculating the said microbes with lactose solution in a reactor and incubating in an aerobic condition to convert lactose into galacto-oligosaccharides. The process also includes separating the galacto-oligosaccharides from the microbes; filtering thus obtained galacto-oligosaccharides; and optionally making the powder form of galacto-oligosaccharides. The process for producing galacto-oligosaccharides provides high yield and high purity.Type: GrantFiled: March 9, 2018Date of Patent: April 19, 2022Assignee: Tata Chemicals LimitedInventors: R Saravanan, S Narayanan, Deepak Jadhav, Manish Jain, Shajahan Shubethar, Shankar Lade, Manoj Gote, Ashok Kumar Dubey
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Patent number: 11273190Abstract: An anti-inflammatory composition of Candida albicans which comprises heat-killed Candida albicans in combination with a neuroprotective supplement. The composition provides a method of stimulating a host immune response capable of reducing a total Candida albicans yeast load below the threshold necessary for the host to keep the Candida albicans yeast and related yeast species in a commensal, non-invasive state. This effect inhibits or prevents chronic inflammation related to invasive Candida genus and allows the composition to be useful for the prevention, prophylaxis, and/or treatment of chronic inflammatory conditions, as well as diseases and disorders of the central nervous system resulting from high L-glutamate levels due to Candida albicans in the gastrointestinal system.Type: GrantFiled: May 6, 2019Date of Patent: March 15, 2022Inventors: Kenda Rigdon, Frank Brady Rigdon
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Patent number: 11117938Abstract: This invention provides a methods and compositions for controlling an insect pest. The methods comprise contacting the insect pest with a composition comprising a yeast cell comprising a nucleic acid encoding a dsRNA molecule which specifically inhibits expression of a target gene in the insect pest, thereby controlling the insect pest.Type: GrantFiled: December 13, 2016Date of Patent: September 14, 2021Assignee: The Regents of the University of CaliforniaInventors: Joanna C. Chiu, Katherine A. Murphy
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Patent number: 11078503Abstract: Provided is a method of producing 3-hydroxyadipic acid, the method including the step of culturing a microorganism belonging to the genus Serratia capable of producing 3-hydroxyadipic acid.Type: GrantFiled: May 30, 2017Date of Patent: August 3, 2021Assignee: TORAY INDUSTRIES, INC.Inventors: Kyohei Isobe, Kenji Kawamura, Masateru Ito, Katsushige Yamada
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Patent number: 10927251Abstract: A solid multiphase polymer blend material comprising: (i) a polyphenolic substance having a molecular weight of at least 500 g/mol; and (ii) a polyester having a molecular weight of at least 500 g/mol; wherein at least a portion of the polyphenolic substance is covalently bonded directly or through a linking moiety to the polyester. Methods for producing the blend material are also described, e.g., homogeneously melt blending a mixture comprising components (i) and (ii) under conditions resulting in covalent attachment of at least a portion of the polyphenolic substance directly or through a linking moiety to the polyester. Methods for producing objects made of the blend material by melt extrusion are also described.Type: GrantFiled: June 25, 2020Date of Patent: February 23, 2021Assignees: UT-Battelle, LLC, University of Tennessee Research FoundationInventors: Anthony S. Bova, Amit K. Naskar
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Patent number: 10519203Abstract: A gene for biosynthesis of core structure of ophiobolin, the gene being the AuOS gene of Aspergillus sp. 094102, deposited with the accession number CCTCC No: M208153, the gene sequence thereof being shown as SEQ ID NO. 1. Also provided is a method of preparation of ophiobolin using the gene.Type: GrantFiled: September 22, 2017Date of Patent: December 31, 2019Assignee: WUHAN UNIVERSITYInventors: Kui Hong, Huiying Meng, Hangzhen Chai, Ru Yin, Zixin Deng
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Patent number: 10260072Abstract: The present application discloses genetically modified yeast cells comprising an active 3-HP fermentation pathway, and the use of these cells to produce 3-HP.Type: GrantFiled: August 28, 2017Date of Patent: April 16, 2019Assignee: CARGILL, INCORPORATEDInventors: Holly Jessen, Brian Rush, Jeanette Huryta, Beth Mastel, Alan Berry, Debbie Yaver, Michael Catlett, Michelle Barnhart
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Patent number: 9957512Abstract: The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production.Type: GrantFiled: July 20, 2017Date of Patent: May 1, 2018Assignee: Verdezyne, Inc.Inventors: Tom Beardslee, Stephen Picataggio, Alex Hutagalung, Tom Fahland
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Patent number: 9765346Abstract: The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production.Type: GrantFiled: September 21, 2016Date of Patent: September 19, 2017Assignee: Verdezyne, Inc.Inventors: Tom Beardslee, Stephen Picataggio, Alex Hutagalung, Tom Fahland
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Patent number: 9724422Abstract: The invention refers to a human lactoferrin derived peptide for use as an antigen masking agent in the production of a pharmaceutical composition for delivery of a biological active substance in a mammalian organism, where the biological active substance is able to induce an undesired immune response by the mammalian organism, where the pharmaceutical composition comprises a supramolecular aggregate of the biological active substance and the human lactoferrin derived peptide, with the effect that after delivery of pharmaceutical composition to the mammalian organism, there is no or only a diminished induction of the undesired immune response against the biological active substance.Type: GrantFiled: November 26, 2010Date of Patent: August 8, 2017Assignee: Evonik Roehm GmbHInventors: Benedikt Hartwig, Juan Tome Alcalde, Norbert Windhab, Maria del Pilar Ansuategui Panzano, Matias Javier Vara Carrera
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Patent number: 9234167Abstract: A system and method for managing an ethanologen for use in biorefinery is disclosed. The method for propagating ethanologen for use in the production of a fermentation product from biomass comprises the steps of providing a medium for propagation of ethanologen and supplying a first cell mass of ethanologen to the medium. A first cell mass of ethanologen is propagated into a larger second cell mass of ethanologen.Type: GrantFiled: March 29, 2013Date of Patent: January 12, 2016Assignee: POET RESEARCH, INC.Inventors: Neelakantam V. Narendranath, David Charles Carlson
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Patent number: 9181563Abstract: The present application discloses the identification of the novel K. marxianus xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of I. orientalis pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.Type: GrantFiled: April 11, 2012Date of Patent: November 10, 2015Assignee: Cargill, IncorporatedInventors: Holly J. Jessen, Jian Yi, Joshua Lundorff, Hans Liao, Ana Negrete-Raymond, Pirkko Suominen, Aristos Aristidou
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Publication number: 20150140629Abstract: The invention relates to an alcoholic fermentation process in the presence of one or more high alcohol tolerant yeast and one or more maltotriose positive yeast. The process of the present invention can be a fermentation process for the production of ethanol, for the production of beer, for the production of wine and the like, in a preferred embodiment, the present invention relates to a process for the production of ethanol in the presence of distiller's yeast and baker's yeast.Type: ApplicationFiled: May 31, 2013Publication date: May 21, 2015Applicant: Cargill, IncorporatedInventors: Jean-Claude Marie Pierre De Troostembergh, Bernhard Horbach, Michael Josef Christian Kruse, Nicolas Andre Albert Meurens
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Publication number: 20150132845Abstract: Devices, systems, and methods for continuous cell culture and other reactions are generally described. In some embodiments, chambers (e.g., cell growth chambers) including at least a portion of a wall formed of a flexible member are provided. A retaining structure can be incorporated outside and proximate to the chamber such that when liquid is added to the chamber, the flexible member is consistently and predictably deformed, and a consistent volume of liquid is added. The flexible member can be formed of, in some embodiments, a gas-permeable medium. In some embodiments, reaction chambers can be arranged in a fluidic loop, and a bypass channel can be used to introduce and/or extract fluid from the loop without affecting loop operation.Type: ApplicationFiled: November 6, 2014Publication date: May 14, 2015Applicant: Massachusetts Institute of TechnologyInventors: Rajeev Jagga Ram, Kevin Shao-Kwan Lee
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Publication number: 20150132740Abstract: A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.Type: ApplicationFiled: May 23, 2013Publication date: May 14, 2015Inventors: Manjiri T. Kshirsagar, Evan Koon Lun Yuuji Hajime, Andrew W. Rabins
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Publication number: 20150125540Abstract: A composition includes an isolated cell; at least one particle within said cell; and at least one active agent associated with the particle, wherein the active agent is capable of being released from the cell. A method includes administration of such a cell to a subject.Type: ApplicationFiled: January 5, 2015Publication date: May 7, 2015Inventors: Jeffrey M. Karp, Debanjan Sarkar, Praveen Kumar Vemula
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Patent number: 9017989Abstract: The invention relates to yeasts expressing the gene BDH1 coding for Bdh1p, characterized in that they catalyze, in an alcoholic fermentation medium, the reduction of acetoin into 2,3-butanediol by a rate at least twice higher than that of the initial stem. The invention can particularly be used for the fermentation of fruit juice and for producing 2,3-butanediol.Type: GrantFiled: May 6, 2008Date of Patent: April 28, 2015Inventors: Sylvie Dequin, Maryam Ehsani, Maria Rosario Fernández Gallegos, Josep A. Biosca, Anne Ortiz-Julien
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Patent number: 9018003Abstract: Disclosed are genes that, when overexpressed in cells expressing alpha-synuclein, either suppress or enhance alpha-synuclein mediated cellular toxicity. Compounds that modulate expression of these genes or activity of the encoded proteins can be used to inhibit alpha-synuclein mediated toxicity and used to treat or prevent synucleinopathies such as Parkinson's disease. Also disclosed are methods of identifying inhibitors of alpha-synuclein mediated toxicity.Type: GrantFiled: May 12, 2006Date of Patent: April 28, 2015Assignee: Whitehead Institute for Biomedical ResearchInventors: Susan L. Lindquist, Aaron D. Gitler, Anil Cashikar
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Publication number: 20150107318Abstract: The method contains the following steps. In step A, supersonic waves are applied to Mountain Litsea fruits for excitation and sterilization. In step B, the inferior fruits and the cleaning water are removed from the water tank, placed in a fermentative tank, and left for fermentation. After the fermentation, a pressing and separation process is conducted. In step C, the superior fruits are placed in a separate fermentative tank for fermentation into mofetil alcohol. In step D, the superior fruits are alternatively mixed with pure water, crushed, and placed in a boiler for aging and sterilization. Then the crushed and aged fruits are placed in yet another fermentative tank with pure water for fermentation. After the fermentation, a pomace separation process is conducted. The separated pomace becomes a Mountain Litsea wine lees, whereas the separated water becomes a Mountain Litsea enzymatic liquid.Type: ApplicationFiled: October 22, 2013Publication date: April 23, 2015Inventors: HENG-JUI HSU, TSU-YU HSU
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Publication number: 20150104822Abstract: The subject matter of the present invention is novel yeast strains capable of metabolizing xylose and resistant to at least one fermentation inhibitor, and also to the method of obtaining same. The subject of the present invention is also the yeasts obtained by culturing said yeast strains and the use thereof for producing at least one fermentation product, preferably ethanol, in particular in a culture medium comprising xylose and at least one fermentation inhibitor.Type: ApplicationFiled: May 24, 2013Publication date: April 16, 2015Inventors: Thomas Desfougeres, Georges Pignede
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Publication number: 20150104868Abstract: The present invention relates to methods of using meso-1,2,3,4-tetrahydroxybutane for the maintenance and/or improvement of biological cell function and activity, and for the prevention of improper cell functioning or cell death, in vitro, ex vivo, and in vivo over time and/or during exposure to stress. Meso-1,2,3,4-tetrahydroxybutane can be used to promote cell survival and as a cell protection agent, to increase cell viability, and to improve conversion of progenitor or stem cells to mature cells, whether in vitro, in vivo, ex-vivo, or transplanted.Type: ApplicationFiled: April 19, 2013Publication date: April 16, 2015Applicant: CARGILL, INC.Inventors: Alvin Berger, Aalt Bast, Petrus Wilhelmus Hubertus De Cock, Gerardus Johannes Martinus Den Hartog, Jean-Claude Marie-Pierre Ghislain De Troostembergh
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Patent number: 9005683Abstract: Provided is a method for producing a yeast containing glutamic acid at a high concentration. In the method for culturing a yeast, a yeast in a stationary growth phase is subjected to liquid culture under the conditions that the pH of a liquid medium is 7.5 or higher and lower than 11. After the pH of the liquid medium for the yeast in a stationary growth phase is adjusted to 7.5 or higher and lower than 11, the yeast is further cultured, whereby a yeast with a high glutamic acid content can be produced. In the invention, as the yeast, Saccharomyces cerevisiae or Candida utilis can be used. Therefore, by using the yeast with a high glutamic acid content obtained by the above-said method and the extract obtained by extraction from the yeast, a seasoning composition and a food or drink with a high glutamic acid content can be provided.Type: GrantFiled: October 30, 2009Date of Patent: April 14, 2015Assignee: Asahi Group Holdings, Ltd.Inventors: Ichiro Shibuya, Hiroaki Okano, Yoshitomo Kanaoka, Nobuchika Takesue
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Publication number: 20150099705Abstract: The present invention relates to Protoxin-II variants, polynucleotides encoding them, and methods of making and using the foregoing.Type: ApplicationFiled: October 3, 2014Publication date: April 9, 2015Inventors: Mack FLINSPACH, Alan Wickenden, Ross Fellows, Robert Neff, Yi Liu, Rebecca Hagan, Qinghao Xu
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Patent number: 8986956Abstract: The present invention relates to a method for producing hEGF (human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.Type: GrantFiled: November 4, 2011Date of Patent: March 24, 2015Assignee: Korea Research Institute of Bioscience and BiotechnologyInventors: Jung Hoon Sohn, Jung Hoon Bae, Mi Jin Kim, Hyun Jin Kim, Soon Ho Park, Kwang Mook Lim
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Patent number: 8980590Abstract: Methods of separating renewable materials, such as lipids, from microorganisms, such as oleaginous yeasts, may include conditioning cell walls of the microorganisms to form, open or enlarge pores, and removing at least a portion of the renewable material through the pores. These methods may result in delipidated microorganisms with cell walls that are substantially intact and with mesopores. These delipidated microorganisms may be used to produce biofuels.Type: GrantFiled: June 28, 2013Date of Patent: March 17, 2015Inventors: Martin J. Sellers, David Jeffers, Jean-Charles Dumenil, Vidya Pai, Jacob Borden
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Publication number: 20150072391Abstract: The present disclosure provides, in various aspects, engineered alcohol tolerant yeast and methods of producing high concentrations of ethanol.Type: ApplicationFiled: September 5, 2014Publication date: March 12, 2015Applicants: Massachusetts Institute of Technology, Whitehead Institute for Biomedical ResearchInventors: Felix Lam, Gerald Fink, Gregory Stephanopoulos
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Patent number: 8969065Abstract: A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.Type: GrantFiled: January 22, 2014Date of Patent: March 3, 2015Assignee: Butamax Advanced Biofuels LLCInventors: Larry Cameron Anthony, Lori Ann Maggio-Hall
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Patent number: 8969011Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.Type: GrantFiled: March 22, 2010Date of Patent: March 3, 2015Assignee: 3M Innovative Properties CompanyInventors: Manjiri T. Kshirsagar, Andrew W. Rabins
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Publication number: 20150056644Abstract: A method and device for growing plant, animal or stem cells in a continuous manner.Type: ApplicationFiled: March 18, 2014Publication date: February 26, 2015Inventor: Eudes Francois Marie DE CRECY
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Patent number: 8956850Abstract: A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.Type: GrantFiled: June 4, 2009Date of Patent: February 17, 2015Assignee: Butamax Advanced Biofuels LLCInventors: Larry Cameron Anthony, Lori Ann Maggio-Hall
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Publication number: 20150037875Abstract: The invention relates to the use of a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having ?-1,3-endoglucanase activity in a process of lysing yeast cell walls as well as to a process for lysing yeast cell walls which comprises exposing said yeast cell walls to a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having ?-1,3-endoglucanase activity.Type: ApplicationFiled: July 13, 2012Publication date: February 5, 2015Applicant: AB ENZYMES GMBHInventors: Kim Langfelder, Volker Marschner, Kornelia Titze, Marika Alapuranen, Jari Vehmaanperä
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Publication number: 20150031078Abstract: The present invention is a Kluyveromyces lactis yeast strain comprising the sequence identified by SEQ ID NO: 1, and methods for the production of sugars (glucose and galactose), ethanol, ?-galactosidase and biomass, in which said Kluyveromyces lactis yeast strain is cultured in the presence of a lactose-containing medium. The lactose-containing medium may be milk, whey, whey resulting from the preparation of butter, whey resulting after casein precipitation, milk permeate, whey permeate, acid whey and YPL culture medium.Type: ApplicationFiled: July 27, 2011Publication date: January 29, 2015Applicant: QUEIZUAR, S.L.Inventors: Angel Pereira Rodriguez, Manuel Becerra Fernandez, Maria Isabel Gonzalez Siso, Maria Esperanza Cerdan Villanueva
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Publication number: 20150024470Abstract: The present invention addresses the problem of providing a new microorganism that is useful for efficiently decomposing oils and fats, and a use for said microorganism. According to screening results, a new Yarrowia lipolytica having a high capacity to assimilate free fatty acids was successfully obtained. Efficient decomposition of oils and fats is achieved by causing the Yarrowia lipolytica to act under conditions in which fatty acids that are hydrolysis products of oils or fats are present, or under conditions in which oils or fats are decomposed into fatty acids and glycerol.Type: ApplicationFiled: January 16, 2013Publication date: January 22, 2015Inventors: Katsutoshi Hori, Masatake Fujioka
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Patent number: 8921082Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.Type: GrantFiled: March 1, 2013Date of Patent: December 30, 2014Assignee: Institute of Nuclear Energy Research Atomic Energy Council, Executive YuanInventors: Chiung-Fang Huang, Yi-Feng Jiang, Ting-Hsiang Lin, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
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Patent number: 8906654Abstract: Described are novel Kluyveromyces marxianus strains NRRL Y-50798 and Y-50799, that were obtained by UV-C irradiation of wild-type K. marxianus NRRL Y-1109 cultures. The UV-C-mutagenized strains were incubated under anaerobic conditions on xylose or glucose medium for a period of 5 months at 46° C. before being selected. These mutagenized strains have potential application in large-scale industrial conversion of lignocellulosic sugars to fuel ethanol given their ability to ferment at temperatures at 46° C. and above.Type: GrantFiled: January 23, 2013Date of Patent: December 9, 2014Assignee: The United States of America, as represented by the Secretary of AgricultureInventor: Stephen R. Hughes
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Publication number: 20140356959Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.Type: ApplicationFiled: June 30, 2014Publication date: December 4, 2014Inventors: George M. CHURCH, Prashant G. MALI, Kevin M. ESVELT
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Publication number: 20140349345Abstract: The present invention relates to a method for the fermentation of cyclopeptides such as pneumocandins in the presence of increased concentrations of any or all of calcium, copper, iron, magnesium, manganese, molybdenum and zinc ions.Type: ApplicationFiled: January 7, 2013Publication date: November 27, 2014Inventors: Wouter Adrianus Van Winden, Rudolf Van Der Pol
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Publication number: 20140342453Abstract: A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.Type: ApplicationFiled: August 5, 2014Publication date: November 20, 2014Inventors: John Shanklin, Tam Huu Nguyen
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Patent number: 8883483Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g.Type: GrantFiled: June 9, 2011Date of Patent: November 11, 2014Assignee: GlycoFi, Inc.Inventors: Tillman U. Gerngross, Stefan Wildt, Byung-kwon Choi, Juergen Nett, Piotr Bobrowicz, Stephen Hamilton, Robert Davidson
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Patent number: 8883470Abstract: A high yield method for fermenting carbohydrate to ethanol, comprising a) treating carbohydrate with a composition containing 10-90 wt % of an aldehyde selected from the group consisting of an formaldehyde, para-formaldehyde, glutaraldehyde and mixtures thereof, 1-50 wt % of a surfactant having an I JLB from 4 to 18, 0-20 wt % of an antimicrobial terpene, or essential oils, 1-50 wt % of organic acids selected from C1-24 fatty acids, their salts, and glyceride esters thereof, and 1-50 wt % water, b) fermenting said carbohydrate in the presence of yeast in a fermentation broth, and c) isolating ethanol in a higher yield than would be obtained without step a).Type: GrantFiled: November 23, 2010Date of Patent: November 11, 2014Assignee: Anitox CorporationInventors: Julio Pimentel, James D. Wilson
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Publication number: 20140329295Abstract: A process including: (a) mixing a microbial biomass, comprising oil-containing microbes, and at least one grinding agent capable of absorbing oil, to provide a disrupted biomass mix; (b) blending at least one binding agent with said disrupted biomass mix to provide a fixable mix capable of forming a solid pellet; and, (c) forming the solid pellet from the fixable mix. The process optionally includes extracting the solid pellet with a solvent to provide an extracted microbial oil.Type: ApplicationFiled: July 18, 2014Publication date: November 6, 2014Inventors: MARIOS AVGOUSTI, TIMOTHY ALLAN BELL, OLIVER WALTER GUTSCHE, KEITH W. HUTCHENSON, ROBERT D. ORLANDI
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Publication number: 20140323757Abstract: The present invention provides a novel compound isolated from yeasts and its use as a bio-surfactant. The bio-surfactant of the present invention shows high surfactant activity, is biodegradable, and is safe to the human body due to its low toxicity. Also, the bio-surfactant of the present invention can be eco-friendly produced through a cultivation of a microorganism in large quantities.Type: ApplicationFiled: July 15, 2013Publication date: October 30, 2014Inventor: Jong Shik Kim
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Publication number: 20140308726Abstract: A high yield method for fermenting carbohydrate to ethanol and prevention and/or disruption of biofilms, comprising: a) mixing a fermentation feedstock with a fermentation broth containing yeast and/or an enzyme, b) treating said mixture by adding a composition to the fermentor containing: 10-90 wt. % of an aldehyde selected from the group consisting of formaldehyde, para-formaldehyde, glutaraldehyde, another antimicrobial aldehyde, and mixtures thereof, 1-50 wt. % of a surfactant having an HLB from 4 to 18, 0-20 wt. % of an antimicrobial terpene, or essential oils, 1-50 wt. % of organic acids selected from Ci to C24 fatty acids, their salts, glycerides and esters thereof, and 1-50 wt. % water; wherein the concentration of aldehyde in the fermentor is from about 0.25 to 3 kg/MT of fermentation feedstock, and c) isolating ethanol.Type: ApplicationFiled: October 29, 2012Publication date: October 16, 2014Inventor: Julio Pimentel
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Publication number: 20140294801Abstract: The present invention provides an improved and specific mRNA interferase and related methods of protein-based mRNA interference.Type: ApplicationFiled: August 3, 2012Publication date: October 2, 2014Applicant: Rutgers, The State University of New JerseyInventors: Masayori Inouye, Yoshihiro Yamaguchi
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Publication number: 20140295543Abstract: The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target.Type: ApplicationFiled: December 16, 2013Publication date: October 2, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Lance FORD, David BROWN
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Publication number: 20140287473Abstract: The present invention relates methods and compositions for producing an organic substance from fermenting microorganism using simultaneous saccharification and fermentation. One aspect of the invention provides producing alcohol by simultaneous saccharification and fermentation by combining a cellulosic substrate, and whole fermentation broth, and an ethanologenic microorganism under conditions conducive both to hydrolysis of cellulose to glucose and to conversion of glucose to alcohol.Type: ApplicationFiled: March 22, 2013Publication date: September 25, 2014Applicant: DANISCO US INC.Inventors: Mian Li, Colin Mitchinson, Landon M. Steele
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Publication number: 20140273168Abstract: Provided are drilling fluids having delay-released lubrication, the drilling fluids comprising a drilling mud and an oleaginous microbial cell, methods of using and making such drilling fluids, and drilling rigs comprising such drilling fluids. Also provided are lubricants comprising an oleaginous microbial cell. Uses for the lubricants include metal working and extreme pressure applications.Type: ApplicationFiled: March 7, 2014Publication date: September 18, 2014Applicant: SOLAZYME, INC.Inventors: Harrison F. DILLON, Frederyk NGANTUNG, Ana Teresita ECHANIZ
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Publication number: 20140273108Abstract: A method of producing hydrolysate from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing and converting complex carbohydrates in the cellulosic material. The hydrolysate produced by the method. Ethanol, organic acids, and organism metabolites produced by the method. Biomass produced by the method. A method of increasing sugar production rate and yield of sugars from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing complex carbohydrates in the cellulosic material and forming sugars.Type: ApplicationFiled: March 17, 2014Publication date: September 18, 2014Applicant: Valicor, IncInventor: James Robert Bleyer