Yeast Patents (Class 435/255.1)
  • Patent number: 10927251
    Abstract: A solid multiphase polymer blend material comprising: (i) a polyphenolic substance having a molecular weight of at least 500 g/mol; and (ii) a polyester having a molecular weight of at least 500 g/mol; wherein at least a portion of the polyphenolic substance is covalently bonded directly or through a linking moiety to the polyester. Methods for producing the blend material are also described, e.g., homogeneously melt blending a mixture comprising components (i) and (ii) under conditions resulting in covalent attachment of at least a portion of the polyphenolic substance directly or through a linking moiety to the polyester. Methods for producing objects made of the blend material by melt extrusion are also described.
    Type: Grant
    Filed: June 25, 2020
    Date of Patent: February 23, 2021
    Assignees: UT-Battelle, LLC, University of Tennessee Research Foundation
    Inventors: Anthony S. Bova, Amit K. Naskar
  • Patent number: 10519203
    Abstract: A gene for biosynthesis of core structure of ophiobolin, the gene being the AuOS gene of Aspergillus sp. 094102, deposited with the accession number CCTCC No: M208153, the gene sequence thereof being shown as SEQ ID NO. 1. Also provided is a method of preparation of ophiobolin using the gene.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: December 31, 2019
    Assignee: WUHAN UNIVERSITY
    Inventors: Kui Hong, Huiying Meng, Hangzhen Chai, Ru Yin, Zixin Deng
  • Patent number: 10260072
    Abstract: The present application discloses genetically modified yeast cells comprising an active 3-HP fermentation pathway, and the use of these cells to produce 3-HP.
    Type: Grant
    Filed: August 28, 2017
    Date of Patent: April 16, 2019
    Assignee: CARGILL, INCORPORATED
    Inventors: Holly Jessen, Brian Rush, Jeanette Huryta, Beth Mastel, Alan Berry, Debbie Yaver, Michael Catlett, Michelle Barnhart
  • Patent number: 9957512
    Abstract: The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production.
    Type: Grant
    Filed: July 20, 2017
    Date of Patent: May 1, 2018
    Assignee: Verdezyne, Inc.
    Inventors: Tom Beardslee, Stephen Picataggio, Alex Hutagalung, Tom Fahland
  • Patent number: 9765346
    Abstract: The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production.
    Type: Grant
    Filed: September 21, 2016
    Date of Patent: September 19, 2017
    Assignee: Verdezyne, Inc.
    Inventors: Tom Beardslee, Stephen Picataggio, Alex Hutagalung, Tom Fahland
  • Patent number: 9724422
    Abstract: The invention refers to a human lactoferrin derived peptide for use as an antigen masking agent in the production of a pharmaceutical composition for delivery of a biological active substance in a mammalian organism, where the biological active substance is able to induce an undesired immune response by the mammalian organism, where the pharmaceutical composition comprises a supramolecular aggregate of the biological active substance and the human lactoferrin derived peptide, with the effect that after delivery of pharmaceutical composition to the mammalian organism, there is no or only a diminished induction of the undesired immune response against the biological active substance.
    Type: Grant
    Filed: November 26, 2010
    Date of Patent: August 8, 2017
    Assignee: Evonik Roehm GmbH
    Inventors: Benedikt Hartwig, Juan Tome Alcalde, Norbert Windhab, Maria del Pilar Ansuategui Panzano, Matias Javier Vara Carrera
  • Patent number: 9234167
    Abstract: A system and method for managing an ethanologen for use in biorefinery is disclosed. The method for propagating ethanologen for use in the production of a fermentation product from biomass comprises the steps of providing a medium for propagation of ethanologen and supplying a first cell mass of ethanologen to the medium. A first cell mass of ethanologen is propagated into a larger second cell mass of ethanologen.
    Type: Grant
    Filed: March 29, 2013
    Date of Patent: January 12, 2016
    Assignee: POET RESEARCH, INC.
    Inventors: Neelakantam V. Narendranath, David Charles Carlson
  • Patent number: 9181563
    Abstract: The present application discloses the identification of the novel K. marxianus xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of I. orientalis pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.
    Type: Grant
    Filed: April 11, 2012
    Date of Patent: November 10, 2015
    Assignee: Cargill, Incorporated
    Inventors: Holly J. Jessen, Jian Yi, Joshua Lundorff, Hans Liao, Ana Negrete-Raymond, Pirkko Suominen, Aristos Aristidou
  • Publication number: 20150140629
    Abstract: The invention relates to an alcoholic fermentation process in the presence of one or more high alcohol tolerant yeast and one or more maltotriose positive yeast. The process of the present invention can be a fermentation process for the production of ethanol, for the production of beer, for the production of wine and the like, in a preferred embodiment, the present invention relates to a process for the production of ethanol in the presence of distiller's yeast and baker's yeast.
    Type: Application
    Filed: May 31, 2013
    Publication date: May 21, 2015
    Applicant: Cargill, Incorporated
    Inventors: Jean-Claude Marie Pierre De Troostembergh, Bernhard Horbach, Michael Josef Christian Kruse, Nicolas Andre Albert Meurens
  • Publication number: 20150132845
    Abstract: Devices, systems, and methods for continuous cell culture and other reactions are generally described. In some embodiments, chambers (e.g., cell growth chambers) including at least a portion of a wall formed of a flexible member are provided. A retaining structure can be incorporated outside and proximate to the chamber such that when liquid is added to the chamber, the flexible member is consistently and predictably deformed, and a consistent volume of liquid is added. The flexible member can be formed of, in some embodiments, a gas-permeable medium. In some embodiments, reaction chambers can be arranged in a fluidic loop, and a bypass channel can be used to introduce and/or extract fluid from the loop without affecting loop operation.
    Type: Application
    Filed: November 6, 2014
    Publication date: May 14, 2015
    Applicant: Massachusetts Institute of Technology
    Inventors: Rajeev Jagga Ram, Kevin Shao-Kwan Lee
  • Publication number: 20150132740
    Abstract: A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.
    Type: Application
    Filed: May 23, 2013
    Publication date: May 14, 2015
    Inventors: Manjiri T. Kshirsagar, Evan Koon Lun Yuuji Hajime, Andrew W. Rabins
  • Publication number: 20150125540
    Abstract: A composition includes an isolated cell; at least one particle within said cell; and at least one active agent associated with the particle, wherein the active agent is capable of being released from the cell. A method includes administration of such a cell to a subject.
    Type: Application
    Filed: January 5, 2015
    Publication date: May 7, 2015
    Inventors: Jeffrey M. Karp, Debanjan Sarkar, Praveen Kumar Vemula
  • Patent number: 9018003
    Abstract: Disclosed are genes that, when overexpressed in cells expressing alpha-synuclein, either suppress or enhance alpha-synuclein mediated cellular toxicity. Compounds that modulate expression of these genes or activity of the encoded proteins can be used to inhibit alpha-synuclein mediated toxicity and used to treat or prevent synucleinopathies such as Parkinson's disease. Also disclosed are methods of identifying inhibitors of alpha-synuclein mediated toxicity.
    Type: Grant
    Filed: May 12, 2006
    Date of Patent: April 28, 2015
    Assignee: Whitehead Institute for Biomedical Research
    Inventors: Susan L. Lindquist, Aaron D. Gitler, Anil Cashikar
  • Patent number: 9017989
    Abstract: The invention relates to yeasts expressing the gene BDH1 coding for Bdh1p, characterized in that they catalyze, in an alcoholic fermentation medium, the reduction of acetoin into 2,3-butanediol by a rate at least twice higher than that of the initial stem. The invention can particularly be used for the fermentation of fruit juice and for producing 2,3-butanediol.
    Type: Grant
    Filed: May 6, 2008
    Date of Patent: April 28, 2015
    Inventors: Sylvie Dequin, Maryam Ehsani, Maria Rosario Fernández Gallegos, Josep A. Biosca, Anne Ortiz-Julien
  • Publication number: 20150107318
    Abstract: The method contains the following steps. In step A, supersonic waves are applied to Mountain Litsea fruits for excitation and sterilization. In step B, the inferior fruits and the cleaning water are removed from the water tank, placed in a fermentative tank, and left for fermentation. After the fermentation, a pressing and separation process is conducted. In step C, the superior fruits are placed in a separate fermentative tank for fermentation into mofetil alcohol. In step D, the superior fruits are alternatively mixed with pure water, crushed, and placed in a boiler for aging and sterilization. Then the crushed and aged fruits are placed in yet another fermentative tank with pure water for fermentation. After the fermentation, a pomace separation process is conducted. The separated pomace becomes a Mountain Litsea wine lees, whereas the separated water becomes a Mountain Litsea enzymatic liquid.
    Type: Application
    Filed: October 22, 2013
    Publication date: April 23, 2015
    Inventors: HENG-JUI HSU, TSU-YU HSU
  • Publication number: 20150104868
    Abstract: The present invention relates to methods of using meso-1,2,3,4-tetrahydroxybutane for the maintenance and/or improvement of biological cell function and activity, and for the prevention of improper cell functioning or cell death, in vitro, ex vivo, and in vivo over time and/or during exposure to stress. Meso-1,2,3,4-tetrahydroxybutane can be used to promote cell survival and as a cell protection agent, to increase cell viability, and to improve conversion of progenitor or stem cells to mature cells, whether in vitro, in vivo, ex-vivo, or transplanted.
    Type: Application
    Filed: April 19, 2013
    Publication date: April 16, 2015
    Applicant: CARGILL, INC.
    Inventors: Alvin Berger, Aalt Bast, Petrus Wilhelmus Hubertus De Cock, Gerardus Johannes Martinus Den Hartog, Jean-Claude Marie-Pierre Ghislain De Troostembergh
  • Publication number: 20150104822
    Abstract: The subject matter of the present invention is novel yeast strains capable of metabolizing xylose and resistant to at least one fermentation inhibitor, and also to the method of obtaining same. The subject of the present invention is also the yeasts obtained by culturing said yeast strains and the use thereof for producing at least one fermentation product, preferably ethanol, in particular in a culture medium comprising xylose and at least one fermentation inhibitor.
    Type: Application
    Filed: May 24, 2013
    Publication date: April 16, 2015
    Inventors: Thomas Desfougeres, Georges Pignede
  • Patent number: 9005683
    Abstract: Provided is a method for producing a yeast containing glutamic acid at a high concentration. In the method for culturing a yeast, a yeast in a stationary growth phase is subjected to liquid culture under the conditions that the pH of a liquid medium is 7.5 or higher and lower than 11. After the pH of the liquid medium for the yeast in a stationary growth phase is adjusted to 7.5 or higher and lower than 11, the yeast is further cultured, whereby a yeast with a high glutamic acid content can be produced. In the invention, as the yeast, Saccharomyces cerevisiae or Candida utilis can be used. Therefore, by using the yeast with a high glutamic acid content obtained by the above-said method and the extract obtained by extraction from the yeast, a seasoning composition and a food or drink with a high glutamic acid content can be provided.
    Type: Grant
    Filed: October 30, 2009
    Date of Patent: April 14, 2015
    Assignee: Asahi Group Holdings, Ltd.
    Inventors: Ichiro Shibuya, Hiroaki Okano, Yoshitomo Kanaoka, Nobuchika Takesue
  • Publication number: 20150099705
    Abstract: The present invention relates to Protoxin-II variants, polynucleotides encoding them, and methods of making and using the foregoing.
    Type: Application
    Filed: October 3, 2014
    Publication date: April 9, 2015
    Inventors: Mack FLINSPACH, Alan Wickenden, Ross Fellows, Robert Neff, Yi Liu, Rebecca Hagan, Qinghao Xu
  • Patent number: 8986956
    Abstract: The present invention relates to a method for producing hEGF (human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.
    Type: Grant
    Filed: November 4, 2011
    Date of Patent: March 24, 2015
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Jung Hoon Sohn, Jung Hoon Bae, Mi Jin Kim, Hyun Jin Kim, Soon Ho Park, Kwang Mook Lim
  • Patent number: 8980590
    Abstract: Methods of separating renewable materials, such as lipids, from microorganisms, such as oleaginous yeasts, may include conditioning cell walls of the microorganisms to form, open or enlarge pores, and removing at least a portion of the renewable material through the pores. These methods may result in delipidated microorganisms with cell walls that are substantially intact and with mesopores. These delipidated microorganisms may be used to produce biofuels.
    Type: Grant
    Filed: June 28, 2013
    Date of Patent: March 17, 2015
    Inventors: Martin J. Sellers, David Jeffers, Jean-Charles Dumenil, Vidya Pai, Jacob Borden
  • Publication number: 20150072391
    Abstract: The present disclosure provides, in various aspects, engineered alcohol tolerant yeast and methods of producing high concentrations of ethanol.
    Type: Application
    Filed: September 5, 2014
    Publication date: March 12, 2015
    Applicants: Massachusetts Institute of Technology, Whitehead Institute for Biomedical Research
    Inventors: Felix Lam, Gerald Fink, Gregory Stephanopoulos
  • Patent number: 8969011
    Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.
    Type: Grant
    Filed: March 22, 2010
    Date of Patent: March 3, 2015
    Assignee: 3M Innovative Properties Company
    Inventors: Manjiri T. Kshirsagar, Andrew W. Rabins
  • Patent number: 8969065
    Abstract: A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.
    Type: Grant
    Filed: January 22, 2014
    Date of Patent: March 3, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Larry Cameron Anthony, Lori Ann Maggio-Hall
  • Publication number: 20150056644
    Abstract: A method and device for growing plant, animal or stem cells in a continuous manner.
    Type: Application
    Filed: March 18, 2014
    Publication date: February 26, 2015
    Inventor: Eudes Francois Marie DE CRECY
  • Patent number: 8956850
    Abstract: A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.
    Type: Grant
    Filed: June 4, 2009
    Date of Patent: February 17, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Larry Cameron Anthony, Lori Ann Maggio-Hall
  • Publication number: 20150037875
    Abstract: The invention relates to the use of a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having ?-1,3-endoglucanase activity in a process of lysing yeast cell walls as well as to a process for lysing yeast cell walls which comprises exposing said yeast cell walls to a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having ?-1,3-endoglucanase activity.
    Type: Application
    Filed: July 13, 2012
    Publication date: February 5, 2015
    Applicant: AB ENZYMES GMBH
    Inventors: Kim Langfelder, Volker Marschner, Kornelia Titze, Marika Alapuranen, Jari Vehmaanperä
  • Publication number: 20150031078
    Abstract: The present invention is a Kluyveromyces lactis yeast strain comprising the sequence identified by SEQ ID NO: 1, and methods for the production of sugars (glucose and galactose), ethanol, ?-galactosidase and biomass, in which said Kluyveromyces lactis yeast strain is cultured in the presence of a lactose-containing medium. The lactose-containing medium may be milk, whey, whey resulting from the preparation of butter, whey resulting after casein precipitation, milk permeate, whey permeate, acid whey and YPL culture medium.
    Type: Application
    Filed: July 27, 2011
    Publication date: January 29, 2015
    Applicant: QUEIZUAR, S.L.
    Inventors: Angel Pereira Rodriguez, Manuel Becerra Fernandez, Maria Isabel Gonzalez Siso, Maria Esperanza Cerdan Villanueva
  • Publication number: 20150024470
    Abstract: The present invention addresses the problem of providing a new microorganism that is useful for efficiently decomposing oils and fats, and a use for said microorganism. According to screening results, a new Yarrowia lipolytica having a high capacity to assimilate free fatty acids was successfully obtained. Efficient decomposition of oils and fats is achieved by causing the Yarrowia lipolytica to act under conditions in which fatty acids that are hydrolysis products of oils or fats are present, or under conditions in which oils or fats are decomposed into fatty acids and glycerol.
    Type: Application
    Filed: January 16, 2013
    Publication date: January 22, 2015
    Inventors: Katsutoshi Hori, Masatake Fujioka
  • Patent number: 8921082
    Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.
    Type: Grant
    Filed: March 1, 2013
    Date of Patent: December 30, 2014
    Assignee: Institute of Nuclear Energy Research Atomic Energy Council, Executive Yuan
    Inventors: Chiung-Fang Huang, Yi-Feng Jiang, Ting-Hsiang Lin, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
  • Patent number: 8906654
    Abstract: Described are novel Kluyveromyces marxianus strains NRRL Y-50798 and Y-50799, that were obtained by UV-C irradiation of wild-type K. marxianus NRRL Y-1109 cultures. The UV-C-mutagenized strains were incubated under anaerobic conditions on xylose or glucose medium for a period of 5 months at 46° C. before being selected. These mutagenized strains have potential application in large-scale industrial conversion of lignocellulosic sugars to fuel ethanol given their ability to ferment at temperatures at 46° C. and above.
    Type: Grant
    Filed: January 23, 2013
    Date of Patent: December 9, 2014
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventor: Stephen R. Hughes
  • Publication number: 20140356959
    Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.
    Type: Application
    Filed: June 30, 2014
    Publication date: December 4, 2014
    Inventors: George M. CHURCH, Prashant G. MALI, Kevin M. ESVELT
  • Publication number: 20140349345
    Abstract: The present invention relates to a method for the fermentation of cyclopeptides such as pneumocandins in the presence of increased concentrations of any or all of calcium, copper, iron, magnesium, manganese, molybdenum and zinc ions.
    Type: Application
    Filed: January 7, 2013
    Publication date: November 27, 2014
    Inventors: Wouter Adrianus Van Winden, Rudolf Van Der Pol
  • Publication number: 20140342453
    Abstract: A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.
    Type: Application
    Filed: August 5, 2014
    Publication date: November 20, 2014
    Inventors: John Shanklin, Tam Huu Nguyen
  • Patent number: 8883483
    Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g.
    Type: Grant
    Filed: June 9, 2011
    Date of Patent: November 11, 2014
    Assignee: GlycoFi, Inc.
    Inventors: Tillman U. Gerngross, Stefan Wildt, Byung-kwon Choi, Juergen Nett, Piotr Bobrowicz, Stephen Hamilton, Robert Davidson
  • Patent number: 8883470
    Abstract: A high yield method for fermenting carbohydrate to ethanol, comprising a) treating carbohydrate with a composition containing 10-90 wt % of an aldehyde selected from the group consisting of an formaldehyde, para-formaldehyde, glutaraldehyde and mixtures thereof, 1-50 wt % of a surfactant having an I JLB from 4 to 18, 0-20 wt % of an antimicrobial terpene, or essential oils, 1-50 wt % of organic acids selected from C1-24 fatty acids, their salts, and glyceride esters thereof, and 1-50 wt % water, b) fermenting said carbohydrate in the presence of yeast in a fermentation broth, and c) isolating ethanol in a higher yield than would be obtained without step a).
    Type: Grant
    Filed: November 23, 2010
    Date of Patent: November 11, 2014
    Assignee: Anitox Corporation
    Inventors: Julio Pimentel, James D. Wilson
  • Publication number: 20140329295
    Abstract: A process including: (a) mixing a microbial biomass, comprising oil-containing microbes, and at least one grinding agent capable of absorbing oil, to provide a disrupted biomass mix; (b) blending at least one binding agent with said disrupted biomass mix to provide a fixable mix capable of forming a solid pellet; and, (c) forming the solid pellet from the fixable mix. The process optionally includes extracting the solid pellet with a solvent to provide an extracted microbial oil.
    Type: Application
    Filed: July 18, 2014
    Publication date: November 6, 2014
    Inventors: MARIOS AVGOUSTI, TIMOTHY ALLAN BELL, OLIVER WALTER GUTSCHE, KEITH W. HUTCHENSON, ROBERT D. ORLANDI
  • Publication number: 20140323757
    Abstract: The present invention provides a novel compound isolated from yeasts and its use as a bio-surfactant. The bio-surfactant of the present invention shows high surfactant activity, is biodegradable, and is safe to the human body due to its low toxicity. Also, the bio-surfactant of the present invention can be eco-friendly produced through a cultivation of a microorganism in large quantities.
    Type: Application
    Filed: July 15, 2013
    Publication date: October 30, 2014
    Inventor: Jong Shik Kim
  • Publication number: 20140308726
    Abstract: A high yield method for fermenting carbohydrate to ethanol and prevention and/or disruption of biofilms, comprising: a) mixing a fermentation feedstock with a fermentation broth containing yeast and/or an enzyme, b) treating said mixture by adding a composition to the fermentor containing: 10-90 wt. % of an aldehyde selected from the group consisting of formaldehyde, para-formaldehyde, glutaraldehyde, another antimicrobial aldehyde, and mixtures thereof, 1-50 wt. % of a surfactant having an HLB from 4 to 18, 0-20 wt. % of an antimicrobial terpene, or essential oils, 1-50 wt. % of organic acids selected from Ci to C24 fatty acids, their salts, glycerides and esters thereof, and 1-50 wt. % water; wherein the concentration of aldehyde in the fermentor is from about 0.25 to 3 kg/MT of fermentation feedstock, and c) isolating ethanol.
    Type: Application
    Filed: October 29, 2012
    Publication date: October 16, 2014
    Inventor: Julio Pimentel
  • Publication number: 20140295543
    Abstract: The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target.
    Type: Application
    Filed: December 16, 2013
    Publication date: October 2, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Lance FORD, David BROWN
  • Publication number: 20140294801
    Abstract: The present invention provides an improved and specific mRNA interferase and related methods of protein-based mRNA interference.
    Type: Application
    Filed: August 3, 2012
    Publication date: October 2, 2014
    Applicant: Rutgers, The State University of New Jersey
    Inventors: Masayori Inouye, Yoshihiro Yamaguchi
  • Publication number: 20140287473
    Abstract: The present invention relates methods and compositions for producing an organic substance from fermenting microorganism using simultaneous saccharification and fermentation. One aspect of the invention provides producing alcohol by simultaneous saccharification and fermentation by combining a cellulosic substrate, and whole fermentation broth, and an ethanologenic microorganism under conditions conducive both to hydrolysis of cellulose to glucose and to conversion of glucose to alcohol.
    Type: Application
    Filed: March 22, 2013
    Publication date: September 25, 2014
    Applicant: DANISCO US INC.
    Inventors: Mian Li, Colin Mitchinson, Landon M. Steele
  • Publication number: 20140273108
    Abstract: A method of producing hydrolysate from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing and converting complex carbohydrates in the cellulosic material. The hydrolysate produced by the method. Ethanol, organic acids, and organism metabolites produced by the method. Biomass produced by the method. A method of increasing sugar production rate and yield of sugars from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing complex carbohydrates in the cellulosic material and forming sugars.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Applicant: Valicor, Inc
    Inventor: James Robert Bleyer
  • Publication number: 20140273168
    Abstract: Provided are drilling fluids having delay-released lubrication, the drilling fluids comprising a drilling mud and an oleaginous microbial cell, methods of using and making such drilling fluids, and drilling rigs comprising such drilling fluids. Also provided are lubricants comprising an oleaginous microbial cell. Uses for the lubricants include metal working and extreme pressure applications.
    Type: Application
    Filed: March 7, 2014
    Publication date: September 18, 2014
    Applicant: SOLAZYME, INC.
    Inventors: Harrison F. DILLON, Frederyk NGANTUNG, Ana Teresita ECHANIZ
  • Patent number: 8835152
    Abstract: Compositions of peptides and surface-active agents are described, as are methods of making and using such compositions. The compositions are capable of affecting metabolic rates in biological systems, and to accelerate nutrient uptake without a concomitant increase in biofilm production.
    Type: Grant
    Filed: December 4, 2012
    Date of Patent: September 16, 2014
    Assignee: Advanced Biocatalytics Corporation
    Inventor: Carl W. Podella
  • Patent number: 8835131
    Abstract: The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.
    Type: Grant
    Filed: March 6, 2012
    Date of Patent: September 16, 2014
    Assignee: University of Kentucky Research Foundation
    Inventors: Joseph Chappell, Bryan Greenhagen
  • Patent number: 8821949
    Abstract: An industrial method of reprocessing a mixture of by-products produced during biodiesel production containing a glycerol fraction and degumming residue as well as a feed additive, which may be obtained by this method.
    Type: Grant
    Filed: April 22, 2009
    Date of Patent: September 2, 2014
    Assignee: Skotan Spolka Akcyjna
    Inventors: Franciszek Baszczok, Waldemar Rymowicz
  • Patent number: 8821892
    Abstract: Disclosed are compositions, including vaccines, and methods for vaccinating an animal against hepatitis C virus (HCV) and for treating or preventing hepatitis C viral infection in an animal. The invention includes a variety of novel HCV fusion proteins that can be used directly as a vaccine or in conjunction with a yeast-based vaccine vehicle to elicit an immune response against HCV in an animal. The invention also includes the use of the HCV fusion gene and protein described herein in any diagnostic or therapeutic protocol for the detection and/or treatment or prevention of HCV infection.
    Type: Grant
    Filed: January 8, 2013
    Date of Patent: September 2, 2014
    Assignee: GlobeImmune, Inc.
    Inventors: Richard C. Duke, Alex Franzusoff, Aurelia Haller, Thomas H. King
  • Patent number: 8815548
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: August 26, 2014
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Alex T. Nielsen, Dmitrii V. Vaviline
  • Publication number: 20140235500
    Abstract: A system for the high-throughput screening of mechanical properties of cells and/or particles is provided. In certain embodiments the system comprises a first structure comprising a plurality of sample receiving wells; a second structure comprising a plurality of exit ports; a porous structure disposed between said plurality of sample receiving wells and said plurality of exit ports and in communication with said plurality of sample receiving wells and in communication with said plurality of exit ports, and said first structure, said second structure and said porous structure are sealed or configured such that when sufficient pressure is applied to sample receiving wells in said first structure said cells or particles migrate through the porous structure into said exit ports, and wherein the mean or the median pore size of said porous structure is smaller than the mean or median cross-section of a cell or particle that is to be screened.
    Type: Application
    Filed: October 15, 2012
    Publication date: August 21, 2014
    Applicant: The Regents of the University of California
    Inventors: Amy Rowat, Dongping Qi