Abstract: This invention relates to bacteria of the genus Pseudonocardia, and in particular the strain deposited on Jul. 12, 2012 at the Pasteur Institute (CNCM, 25 rue du Docteur Roux, F-75724 PARIS Cedex 15, France) under No. CNCM 1-4656, capable of degrading MTBE and/or ETBE. The invention also relates to a process for treatment of an effluent comprising MTBE and/or ETBE and optionally TBA that uses such bacteria.

Abstract: Accelerated dechlorination of soil and water contaminated with chlorinated solvents in situ is achieved by delivering ferric ammonium citrate into the soils and/or water. The induction of ferric ammonium citrate into sulfate-rich reducing conditions initiates a combined abiotic and biotic mechanism for the dechlorination of subsurface contaminants. Initial and rapid removal of chlorinated solvents is achieved by way of reductive transformation, a mechanism utilizing the creation of an iron-bound soil mineral (pyrite) followed by stimulating conditions for enhanced biological natural attenuation.

Abstract: A method for treating an organic and/or inorganic substrate utilizing a granular material made of a solid foam as support for an active component, for example a biocatalyst such as a microorganism or an enzyme. The solid foam has a continuous phase in which magnetizable particles are embedded, such that the support with the biologically active component immobilized thereon can be separated from a mixture with a magnetic separation device.

Abstract: A Capture Carbon Storage (CCS) Process for the economical capture of carbon dioxide from coal fuel gas, and the storage of the carbon dioxide as lipid oil or the use of the environmentally begin oil for transportation. The lipid oil may be refined into gasoline, biodiesel fuel, jet fuel, ethanol, and methane which provide a renewable energy resource for the United State's future energy needs. It is a new fuel form which has been both chemically and physically altered to reduce the emissions of carbon dioxide. It is a coal cleaning/upgrading process which produces a refined fuel, Hydrogen, that may be used to produce electricity and an environmentally begin biofuel for transportation. The renewable fuel will produce no carbon foot print at the internal combustion engine's exhaust pipe. The CCS Process will produce a 100% reduction in carbon dioxide (CO2) emissions into the atmosphere and thereby, stop the Global Warming.

Abstract: The bacterium that can perform complete dechlorination of trichloroethene, and the bacteria consortium containing the bacterium are provided, and the method to dechlorinate soils or ground water contaminated with trichloroethene using the bacterium is also provide.

Abstract: The present invention relates to a method for the display of recombinant functional polypeptides containing a prosthetic group selected from metal porphyrin and flavin containing groups on the surface of a host cell using the transporter domain of an autotransporter.

Abstract: The present invention relates to a novel microbial strain, Alteromonas sp. SN2 (KCTC11700BP) having an activity for degrading polycyclic aromatic hydrocarbon (PAH), a bioremediation process of polycyclic aromatic hydrocarbon (PAH) contaminants, comprising contacting a microbial strain Alteromonas sp. with PAH, and a composition for purifying polycyclic aromatic hydrocarbon (PAH) contaminants, comprising a microbial strain Alteromonas sp. It is advantageous that the present invention may be suitably utilized in treatment or remediation of oil contaminants, particularly naphthalene soil contaminants without production of a secondary contaminant source. As the strain and bioremediation process of this invention could be industrially utilized through low-cost and environment-friendly process, the present technology also may be carried out much more economically and efficiently than conventional technologies such as thermal technology, solidification technology, and vapor extraction technology.

Abstract: Described herein are compounds, compositions, and methods useful for bioremediation of a contamination. In particular, described herein are compositions that include one or methyl esters of a fatty acid and one or more bioremediation reagents, and methods for their use.

Abstract: Certain embodiments are directed to methods of enhancing the petroleum degrading abilities of bacteria and the resulting bacterial composition. In certain aspects the bacteria are selected for degradation of a petroleum having a particular composition profile and/or for environmental robustness.

Abstract: Excess sludge production in a bioreactor of a wastewater treatment plant in which excess sludge is being produced is reduced by adding an activated sludge material having a chitinase specific activity of at least 150 Units/g-MLSS and a pectinase specific activity of at least 120 Units/g-MLSS to the bioreactor. After the addition of the activated sludge material, the bioreactor is controlled by adding the activated sludge material when any one of the chitinase activity, the pectinase activity, and the protease specific of the sludge in the bioreactor drops below the lower limits of 50 Units/L, 40 Units/L, and 0.3 Units/L, respectively.

Abstract: The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked organophosphate-degrading enzyme having a support material which includes a biomass material and/or a polymeric material. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized organophosphate hydrolase enzyme and enzyme materials.

Abstract: The present invention relates to a Sphingomonas sp. MD2 strain (KCTC 11845BP), a composition including the strain for decomposing methane or odor-producing compounds, a biocover or biofilter including the composition, a method for decomposing methane or odor-producing compounds using the composition, a system for decomposing methane or odor-producing compounds using the biocover or biofilter, and the use of the strain for decomposing methane or odor-producing compounds. According to the present invention, methane and odor can be effectively removed concurrently, and thus the cost required for the separate treatment of methane and odor can be reduced, and methane and odor-producing compounds in landfills or the like can be effectively decomposed.

Abstract: The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked enzyme having a support material which includes a biomass material different than the biomass used to initially derive the enzyme. Optionally, the immobilized enzyme further includes a polymeric material and/or the biomass which was used to initially derive the enzyme. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized enzyme materials.

Abstract: A method and apparatus are provided for conducting a microbiological process on a bulk material in which a quantity of the bulk material is loaded onto a waterproof lining forming part of a bio cell with a heat transfer arrangement below the quantity of bulk material or within its volume, or both. The moisture content of the bulk material is controlled by periodic or intermittent distribution of water into the bulk material in order to promote microbiological activity within the bulk material by means of microbes that may be either naturally occurring within the bulk material or may be selected and introduced into the bulk material according to a desired result. A leachate recovery installation collects leachate draining from the bulk material. The temperature within the bulk material is monitored and the temperature controlled in order to elevate or decrease the temperature thereof to approach a target temperature associated with enhanced microbial activity of microbes present within the bulk material.

Abstract: A packet of an enzyme and/or enzyme producing bacteria is contained within the core of a roll of toilet tissue or similar paper product wound on a core. The packet has a cover that dissolves or disintegrates on contact with water and disperses its contents into the aqueous waste stream. The packet may contain a mixture of bacterial cultures that produce enzymes to attack the greasy or fatty components of the waste stream. An additional article such as a sample of a liquid or creme personal care product may also be contained within the tissue paper core.

Abstract: An artificial biomembrane and a method for manufacturing the same. Raw film-forming materials, such as polyvinyl alcohol, sodium alginate and sodium carboxymethyl cellulose, are dissolved in water to form a glue liquid, into which a mixture of coal-based activated carbon powder, diatomaceous earth powder sodium acetate and single strain or multiple strains of microbial cells are added. The resultant glue liquid is immobilized using powder beds or fluidized beds to form artificial biomembranes. The artificial biomembranes can be manufactured in a large-scale, with simple process of immobilization, high efficiency and low cost. The artificial biomembranes can be applied to ecological restorations and different polluted water environments, such as industrial wastewater, sewage, fishing water, landscape water, flowing water and the like.

Abstract: The present application relates to a method of degrading organic contaminants in contaminated soil, sediment or wastewater, the method being carried out by treating the contaminated soil, sediment or wastewater with thermophilic bacterium capable of degrading the organic contaminants.

Abstract: A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

Abstract: A microbial technology package is disclosed for the reclamation of sodic alkali soils. The method comprises forming an aqueous solution containing mixed microbial composition. The fungus Trichoderma was also included in the technology along with several biological amendments such as molasses and farmyard manure. The technology being based on microorganisms, the treatment is effective and reclaims the soil up to deeper layers. Soils reclaimed by means of microbial treatment may leach out the excess sodium from clay particle improving the soil aggregation, thereby improving water holding capacity and texture of soil. The complete microbial technology package provided in the present invention was successfully reclaimed the soil to normal fertile soil.

Abstract: Isolated microbial consortia capable of degrading chlorinated carbohydrates and a method to acclimatize microbes to degrade chlorinated carbohydrates under specific conditions of temperature and salt are described. Also described is a method for using microbial consortia to degrade chlorinated carbohydrates in a waste stream.

Abstract: A method for the degradation of polycyclic aromatic compounds is disclosed that involves dissolving ozone in a bipolar solvent comprising a non-polar solvent in which is of sufficiently non-polar character to solubilized the polycyclic aromatic compounds, and a polar-water-compatible solvent which is fully miscible with the non-polar solvent to form a single phase with the non-polar solvent. The bipolar solvent with dissolved ozone is contacted with the polycyclic aromatic compounds to solubilize the polycyclic aromatic compounds and react the dissolved polycyclic aromatic compounds with the ozone to degrade the dissolved polycyclic aromatic compounds to oxygenated intermediates. The bipolar solvent is then mixed with sufficient water to form separate non-polar and polar phases, the non-polar phase comprising the non-polar solvent and the polar phase comprising the non-polar solvent and the oxygenated intermediates.

Abstract: Provided is Sphingomonas paucimobilis (SEQ ID NO. 1) having base sequences of which a sequence number is 1, being deposited with a deposition number KCTC 11771BP, and being used for specifically measuring aromatic hydrocarbons as a contaminant in a sample.

Abstract: Systems, compositions, and methods for the bioremediation of a contaminant contained within a contaminated region. The systems, compositions, and methods may include supplying a first bioremediation formulation that includes an ion exchange resin to a first treatment zone that is associated with the contaminated region. The systems, compositions, and methods further may include supplying a second bioremediation formulation that includes a high-mobility oxidant, a low-mobility oxidant, and a nutrient material to a second treatment zone that is associated with the contaminated region. The systems, compositions, and methods also may include a kit of bioremediation formulations that includes the first bioremediation formulation and the second bioremediation formulation and is to be utilized during the bioremediation of the contaminated region.

Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.

Abstract: Disclosed are new molecules, on a peptide-porphyrin base, with a low molecular weight (2000-5000 uma), optionally in covalent association with biomolecules, which are able to catalyse peroxidation, oxidation, hydroxylation, phenol nitration and inert compound epoxidation reactions, using clean reagents such as H2O2 in aqueous or water-alcohol solutions, with catalytic efficiency comparable or superior to that of natural enzymes. The compounds according to the invention can be used as catalysts in fine chemistry, in the control and decontamination of waters and laboratory diagnostics, and may be bonded and/or adsorbed on solid matrices or nanoparticles.

Abstract: The present invention relates to the use or process of use of a sequencing batch reactor for eliminating prion in specified risk materials and for measuring the efficacy of a sequencing batch reactor to degrade prion proteins in specified risk materials.

Abstract: The present invention is a kit and method for bioremediation of deicing fluids containing glycol. Using a combination Bacillus, Pseudomonas, yeast and filamentous fungi strains selected for the ability to grow on high levels of glycol, bioremediation of airport runoff is provided.

Abstract: The invention relates to methods for inactivating antibiotics in the environment. The invention further relates to the use of a laccase, a lipase or a cellulase for inactivation of antibiotics. It also relates to compositions to decontaminate the polluted environment and to prevent the pollution of the environment by inactivating antibiotics from waste and wastewater effluents before they reach the environment.

Abstract: A method of degrading carbon nanomaterials includes mixing the carbon nanomaterials with a composition comprising a peroxide substrate and at least one catalyst selected from the group of an enzyme and an enzyme analog. The peroxide substrate undergoes a reaction in the presence of the catalyst to produce an agent interactive with the nanotubes to degrade the carbon nanomaterials. The peroxide substrate can, for example, be hydrogen peroxide or an organic peroxide.

Abstract: A method for creating Class A compost for sale to the public from waste products, especially municipal sewage, involving lower expense and improved versatility over prior methods.

Abstract: Sorption reinforced catalytic coating system for the degradation of threat agents including a synzyme coating about a material, the synzyme coating having bucket-shaped molecules for the sorption and degradation of the threat agents. A binding agent is configured for synzyme immobilization to maximize loading and retention of the synzyme coating on the material.

Abstract: Sorption reinforced catalytic coating system for the degradation of threat agents including a synzyme coating about a material configured for the degradation of the threat agents, the synzyme coating including bucket-shaped molecules configured for the sorption of the threat agents A binding agent is configured for synzyme immobilization to maximize loading and retention of the synzyme coating on the material.

Abstract: The use or process of use of a sequencing batch reactor for eliminating prion in specified risk materials and for measuring the efficacy of a sequencing batch reactor to degrade prior proteins in specified risk materials are disclosed. The specified risk material is fed to a sequencing batch reactor containing a layer of acclimatized anaerobic sludge, and the specified risk material reacts with the sludge at a temperature below 25° C. so as to allow degradation of the prion protein.

Abstract: Provided herein are methods and products for biomass digestion, which includes the production of biogas, U.S. Environmental Protection Agency classified Class A Biosolids, and pathogen reduced organic liquid fertilizer. Through the digestion of waste materials using sequential phases in an efficient digestion process, enhanced biomass conversion efficiency and improved output of products (in quantity and/or quality) are obtained with a significant reduction in dwell time in each phase.

Abstract: Disclosed are methods, compositions and kits pertaining to polymer coatings that entrap enzymes, specifically enzymes capable of degrading bisphenol A.

Abstract: The aim of the invention is to use sulphate-reducing thermophilic archaeobacteria for carrying out a method for degrading linear or branched, saturated or unsaturated, when necessary sulphur, aromatic hydrocarbons under anaerobic conditions.

Abstract: Methods and systems for dehalogenating organohalides are disclosed. In one respect, the systems can generate hydrogen in an electrolysis cell and supply the hydrogen to anaerobic dehalogenating bacteria to decontaminate organohalides at a contamination site.

Abstract: A plant for producing an oxygen-containing additive for liquid motor fuels comprises an anaerobic fermentation vessel, a gasholder, a system for removal of sulphuretted hydrogen, and a hotwell. The plant further comprises an aerobic fermentation vessel, a device for liquid substance pumping, a device for liquid aeration with an oxygen-containing gas, a removal system of solid mass residue after fermentation, a gas distribution device; a device for heavy gases utilization; a device for ammonia adsorption by water; a liquid-gas mixer; a cavity mixer, a system that serves superficial active and dispersant matters and a cooler; all of these being connected to each other by pipelines. The technical result being the implementation of a process for producing an oxygen containing additive, which after being added to liquid motor fuels, provides an ecologically beneficial component for motor fuels by ensuring the stability of composition fuel properties during long-term storage.

Abstract: A method for enhancing biotransformation of groundwater contaminants is provided. The method comprises steps of providing an injection column at an upstream of a contaminated source for injecting a compost solid; providing a recirculation device at a downstream of the contaminated source; forming a compost liquid by mixing surface water or groundwater from the recirculation device with the compost solid, and the groundwater bringing the compost liquid to the contaminated source at the downstream; and producing a reaction at the contaminated source and the downstream. Also, the groundwater contaminants can be adsorbed and biodegraded in the injection column at the upstream by the recirculation of the recirculation device.

Abstract: A method of in situ treatment of fracture fluid that is contaminated with a heavy metal may include passing a flow of contaminated fracture fluid through a reactor vessel. The reactor vessel may contain a plurality of biofilm retaining structures. Each of the structures may at least be partially coated with a biofilm. Additionally, the biofilm may include a plurality of bacteria retained in a matrix and the matrix may include DNA, protein and carbohydrates produced by the bacteria.

Abstract: This invention relates to molecular and cellular biology and biochemistry. In one aspect, the invention provides polypeptides having cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or ?-glucosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or ?-glucosidase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts.

Abstract: A method of in situ treatment of wastewater that is contaminated with a heavy metal may include passing a flow of contaminated wastewater through a reactor vessel. The reactor vessel may contain a plurality of biofilm retaining structures. Each of the structures may at least be partially coated with a biofilm. Additionally, the biofilm may include a plurality of bacteria retained in a matrix and the matrix may include DNA, protein and carbohydrates produced by the bacteria.

Abstract: The mat is formed of a saprophytic fungal strain and/or budding yeast, and a mass of particles wherein the fungus is characterized in producing an enzyme capable of breaking down polycyclic aromatic hydrocarbons. In a second embodiment, a mass of pellets is made from the mat for use in absorbing liquid animal waste.

Abstract: To provide an apparatus for treating a radioactive nitrate waste liquid that includes a denitrification tank (12A) which accommodates active sludge that adsorbs or takes in the radioactive substance in a nitrate waste liquid (11) and in which an anaerobic microorganism that reduces the nitrate to nitrogen gas grows, and a reaeration tank (14) in which a denitrification-treated liquid (24) treated in the denitrification tank (12A) is aerated and mixed with active sludge. A pH adjuster (21), a carbon source (22), and nitrogen gas are supplied to the denitrification tank (12A) so as to separate a denitrified liquid into a solid content and the denitrification-treated liquid (24) by using a first solid-liquid separating film (25), and the denitrification-treated liquid (24) treated with the active sludge in the reaeration tank (14) is reaerated and separated into a solid content and a reaeration-treated liquid (27) by using a second solid-liquid separating film (28).

Abstract: The invention relates to novel algae of the Coccomyxa genus, in particular the algae of a novel species called Coccomyxa actinabiotis, and to the use thereof for metal uptake from aqueous media, and in particular from radioactive media.

Abstract: The present invention discloses an isolated pure culture of a novel strain CK1 of Bacillus licheniformis, DSM 025954, capable of detoxification of zearalenone (ZEN). Using physiological, biochemical, morphological identification and 16S rRNA gene sequence analysis methods, the strain CK1 was identified as Bacillus licheniformis. Through extracellular xylanase, CMCase protease assays and evaluations for zearalenone detoxification, the strain CK1 strain was identified to possess good ZEN-detoxifying ability, to be non-hemolytic, non-enterotoxin producing, and displayed high levels of extracellular xylanase, cellulase, and protease activities. Accordingly, Bacillus licheniformis CK1 can be applied as food and feed supplement for bio-detoxification of ZEN.

Abstract: The present invention relates to compositions and methods for the bioremediation of hydrocarbon-containing waste, such as drill mud and drill cuttings from oil and gas wellbores, drill waste deposit sites, oil spills, oil and gas production waste, and contaminated surfaces, using wood chips comprising microorganisms of mountain pine beetle.

Abstract: A method for enhancing degradation of petroleum and its derivatives in an object includes placing an object containing petroleum and its derivatives into a compartment of a tank. Oil-eating bacteria is added into or cultivated in the compartment. A suction motor is activated to suck air out of the compartment, such that an air pressure in the compartment is smaller than an air pressure outside of the compartment, forcing the air outside of the compartment to flow into the compartment. A device for enhancing degradation of petroleum and its derivatives in an object includes a tank having a space and an outlet in communication with a bottom of the compartment. The tank includes a top side having an opening in communication with the compartment. A suction motor includes an air inlet and an air outlet. The outlet of the tank is connected to the air inlet of the suction motor.

Abstract: The present invention relates to a method for treatment of sludge in water purification, the method comprising the steps: providing an enzyme mixture comprising at least one enzyme capable of digesting natural polymeric materials; and adding the enzyme mixture and at least one chelating reagent, in optional order or simultaneously, to an aqueous sludge suspension.

Abstract: The invention provides methods for in situ acceleration of biological degradation of chlorinated hydrocarbons in a soil. The invention particularly provides a method for in situ acceleration of biological degradation of chlorinated hydrocarbons in a soil comprising adding H2, CO2 and acetate.