Measuring Or Testing For Antibody Or Nucleic Acid, Or Measuring Or Testing Using Antibody Or Nucleic Acid Patents (Class 435/287.2)
  • Patent number: 11925478
    Abstract: Provided is an implantable biosensor including an intermediate layer; a first electrode layer provided on one surface of the intermediate layer and including a first electrode configured to react with a bio material and an auxiliary electrode electrically connected to the first electrode; and a second electrode layer provided on another surface of the intermediate layer to face the first electrode layer and including a second electrode operating as a reference electrode.
    Type: Grant
    Filed: February 18, 2020
    Date of Patent: March 12, 2024
    Assignee: DAEGU GYEONGBUK INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Kyung In Jang, Jong Cheol Rah, Han Hee Jung
  • Patent number: 11906513
    Abstract: It is an object of the present invention to provide a method or an immunochromatographic test piece, which controls a speed or a direction of development of a specimen on the immunochromatographic test piece, so that a treatment with an acid reagent, nitrite, and a neutralizing reagent is properly controlled. The present invention provides an immunochromatographic test piece for extracting and measuring a sugar chain antigen in a specimen, the immunochromatographic test piece having a neutralizing region impregnated with a neutralizing reagent upstream of a label region, and further having a solid acid reagent or nitrite region impregnated with a solid acid reagent, or with nitrite, upstream of the neutralizing region, wherein a resin-made sheet is sandwiched between the solid acid reagent or nitrite region the neutralizing region so as to suppress the movement of a reagent or the movement of a specimen solution between the regions.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: February 20, 2024
    Assignee: Denka Company Limited
    Inventors: Daisuke Kato, Shino Muramatsu, Tomohiro Hattori
  • Patent number: 11890620
    Abstract: Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.
    Type: Grant
    Filed: October 12, 2018
    Date of Patent: February 6, 2024
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Kirsty A. McFarland, Charles A. Lissandrello, Andrew P. Magyar, Erin Rosenberger
  • Patent number: 11892425
    Abstract: The embodiments disclose an apparatus including a test cartridge configured for inserting into a reader, a sample insertion component coupled to the test cartridge a test sample, a heater device coupled to the test cartridge configured to heat to a predetermined temperature the test sample, a sensor array coupled to the test cartridge consisting of at least one electrochemical sensor for sensing analytes in a sample, a reader configured to gather test related data from the sensor array coupled to the test cartridge, an analyzer coupled to the reader configured for determining test results of each sensor in the sensor array and comparing all the test results for confirmation of a valid test and coherent results, a MCU (Microcontroller Unit) to perform main control and processing functions, and orchestrates all the functionality for the Reader, and a BLE Radio RF link between the MCU and an external processing system.
    Type: Grant
    Filed: October 19, 2021
    Date of Patent: February 6, 2024
    Inventors: Matthew Hummer, Gregory J. Hummer
  • Patent number: 11883824
    Abstract: Disclosure relates to a device and a method of using the device for conducting biological and chemical assays on a sample. The device includes two plates. The first plate includes at least a first and a second sample contact areas on its inner surface. Each sample contact area includes spacers having a bottom end fixed on the inner surface and a top end that is distal to the inner surface. The spacers in the first sample contact area differ in height from those in the second sample contact area. The first and second sample contact areas are disposed at different depths along the inner surface of the first plate, so that the top ends of the spacers are aligned on the same flat plane. The method includes depositing the sample onto the sample contact areas, compressing the sample into a thin layer, and analyzing the sample.
    Type: Grant
    Filed: February 9, 2018
    Date of Patent: January 30, 2024
    Assignee: Essenlix Corporation
    Inventors: Stephen Y. Chou, Wei Ding, Ji Qi, Yufan Zhang
  • Patent number: 11877731
    Abstract: An analytical toilet comprising a bowl adapted to receive excreta; a channel in fluid communication with the bowl for extracting a sample of excreta from the bowl; an imaging section in the channel, comprising a transparent window; and a movable wall capable of moving between a first and a second position; wherein the channel is open when the movable wall is in a first position; and wherein the channel is nearly closed when the movable wall is in a second position; a first actuator adapted to move the movable wall between its first and second position; an imaging sensor positioned to image the contents of the channel at the point of the movable wall through the transparent window is disclosed.
    Type: Grant
    Filed: March 8, 2021
    Date of Patent: January 23, 2024
    Assignee: Hall Labs LLC
    Inventors: David R. Hall, Jared Reynolds, Jared Blake, Joshua Larsen
  • Patent number: 11863041
    Abstract: The invention provides devices and methods for moving charged molecules into and out of tissue samples. This invention is particularly useful for removing endogenous heterogenous particles from tissue samples and for introducing exogenous charged molecules (e.g., antibodies, dyes) into tissue samples.
    Type: Grant
    Filed: August 2, 2019
    Date of Patent: January 2, 2024
    Assignee: University of Massachusetts
    Inventors: Joseph Fossland Bergan, Joseph Frank Dantas Dwyer
  • Patent number: 11850588
    Abstract: A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.
    Type: Grant
    Filed: July 5, 2022
    Date of Patent: December 26, 2023
    Assignee: DUBLIN CITY UNIVERSITY
    Inventors: Robert Forster, Elaine Spain, Kellie Adamson, Eadaoin Carthy, David Boyle
  • Patent number: 11851685
    Abstract: Described herein are reagent compositions for detecting and/or measuring analytes in a test sample. In one embodiment, reagent compositions are described for detecting and/or measuring glucose in a sample.
    Type: Grant
    Filed: November 8, 2021
    Date of Patent: December 26, 2023
    Assignee: ROCHE DIABETES CARE, INC.
    Inventors: Nathan Allen Carrington, Stacy Hunt Duvall, Leon Scott Van Dyke
  • Patent number: 11834642
    Abstract: A method for collecting a fine particle stored in a structure by suctioning the fine particle using a nozzle, in which, as the structure, a structure in which at least one communication portion that communicates a space storing the fine particle with one surface side and the other surface side of the structure is formed is used.
    Type: Grant
    Filed: September 21, 2017
    Date of Patent: December 5, 2023
    Assignee: Tokyo Ohka Kogyo Co., Ltd.
    Inventors: Takashi Ohsaka, Yasuo Suzuki, Atsushi Murota
  • Patent number: 11819323
    Abstract: The embodiments disclose a method including making electrodes using an electrically conductive material for impedimetric detection of analytical targets in an electrochemical sensing platform device, collecting patient samples in a solution compartment of the electrochemical sensing platform device, binding primers and aptamers to electrodes for functionalizing electrodes for detecting and binding targeted viruses and bacterial pathogens, incubating oral fluid samples in the solution compartment using a heater, measuring electrode impedance changes, recording measured electrode impedance changes in a memory device, and integrating wireless technologies into the electrochemical sensing platform device configured for transmitting recorded measured data.
    Type: Grant
    Filed: October 18, 2021
    Date of Patent: November 21, 2023
    Inventor: Gregory J. Hummer
  • Patent number: 11821818
    Abstract: A biosensor may provide a magnetoresistive (MR) film comprising a nonmagnetic layer may be sandwiched between the two ferromagnetic layers. The MR film may be positioned on a substrate, where the edges of the MR film are in contact with leads. Additionally, the leads may be in contact with pads. The sensors may provide quasi-digital readout that enable greatly enhanced sensitivity. In some embodiments, biosensors may be arranged as array of sensors. The array of sensors may be arranged as a symmetric or asymmetric N1×N2 array, where N1 and N2 are integers, N1 represents the number of sensors linked together in series, and N2 represents the number of sensor sets in parallel, where each sensor set may comprise one or more sensors. Further, the array of sensors may be coupled to a voltmeter, which may be a single voltmeter in some cases that allows the sensors to all be probed simultaneously.
    Type: Grant
    Filed: July 6, 2018
    Date of Patent: November 21, 2023
    Assignee: University of Houston System
    Inventors: Dmitri Litvinov, Long Chang, Richard Willson
  • Patent number: 11808670
    Abstract: The invention relates to an immunochromatographic device which contains a nitrous acid compound and an organic acid or an organic acid derivative and which is for detecting a detection target in an analyte wherein a sample droplet-receiving member, a labeling substance-holding member, a chromatography medium member and an absorption member are arranged in a manner that a sample develops in this order and wherein a part containing the nitrous acid compound and a part containing the organic acid or the like are at upstream positions from the labeling substance-containing part, and the part containing the nitrous acid compound and the part containing the organic acid or the like are not substantially in contact with each other in the thickness direction.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: November 7, 2023
    Assignee: TANAKA KIKINZOKU KOGYO K.K.
    Inventors: Keita Suzuki, Yuya Kato, Hisahiko Iwamoto
  • Patent number: 11806140
    Abstract: An apparatus for in-vivo measuring H2O2 oxidation within a living tissue. The apparatus includes an electrochemical probe and an electrochemical stimulator-analyzer. The electrochemical probe includes a sensing part and a handle. The sensing part includes a working electrode, a counter electrode, and a reference electrode. The working electrode includes a first biocompatible conductive needle coated with a layer of vertically aligned multi-walled carbon nanotubes. The counter electrode includes a second biocompatible conductive needle. The reference electrode includes a third biocompatible conductive needle. The electrochemical stimulator-analyzer is configured to generate a set of electrical currents in a portion of the living tissue.
    Type: Grant
    Filed: March 20, 2021
    Date of Patent: November 7, 2023
    Inventors: Mohammad Abdolahad, Naser Namdar Habashi, Zohreh Sadat Miripour
  • Patent number: 11806711
    Abstract: Various fluidic structures are described that may be implemented between two portions of material, at least one of which may be made of a flexible but inelastic material, such as Mylar. Such fluidic circuits may be configured such that applying a clamping pressure zone to such fluidic circuits and then moving the clamping pressure zone along the fluidic circuit and along a particular axis may cause the fluidic circuit to operate in a particular manner, thereby driving fluids contained within the fluidic circuit around the fluidic circuit according to a desired flow sequence.
    Type: Grant
    Filed: January 12, 2022
    Date of Patent: November 7, 2023
    Assignee: Hound Labs, Inc.
    Inventors: Daniel H. Friedman, Michael V. D'Ambrosio, William Welch, Thu M. Nguyen
  • Patent number: 11796531
    Abstract: Systems for detecting fluorescence from a molecule comprising an ion-impermeable film comprising at least one ion-conducting nanopore; a first and second liquid reservoir separated by the film; a means to induce movement of the molecule from the first reservoir to the second reservoir via the nanopore; a light source capable of exciting the molecule to emit fluorescence, wherein the light source shines into the second reservoir; a metallic layer adhered to the film by an adhesion layer and comprising a nanowell structure located adjacent to the nanopore; and a detector configured to detect the fluorescence emitted by the molecule are provided. Methods of use of the systems are also provided.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: October 24, 2023
    Assignee: TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED
    Inventors: Amit Meller, Ossama Assad, Tal Gilboa, Joshua Spitzberg
  • Patent number: 11788040
    Abstract: The present invention relates to a device (10; 30; 50) for analysing liquid samples comprising amplified nucleic acids, said device comprising: a sample pad (16); a first (18) and a second (19) sample analysis strip configured to analyse different aspects of the nucleic acid sample wherein said segments comprise nucleic acid sequences which are complementary to the predetermined sequences of target nucleic acids whose presence or absence is analysed; and a housing (11) enclosing said sample pad and at least two elongated sample analysis strips wherein the analysis result is detectable from the outside of the housing from the combination of aligned segments of said first and second analysis strips. The invention furthermore relates to a method for determining the presence or absence of a target nucleic acid, providing confirmatory results, in combination with a predetermined sequence in a sample from a subject using the device according to the invention.
    Type: Grant
    Filed: February 15, 2017
    Date of Patent: October 17, 2023
    Assignee: EMPE DIAGNOSTICS AB
    Inventors: Pavankumar Asalapuram, Mats Nilsson Bernitz
  • Patent number: 11782011
    Abstract: An electrochemical biosensor includes a working electrode modified with a redox polymer and amine-terminated capture aptamer specific for a particular detection target. The binding sequence of the capture aptamer is also complementary to part of a second ssDNA which is labeled with HRP (horseradish peroxidase). The capture aptamer will form dsDNA with the HRP-labeled ssDNA and bring HRP into electrical contact with the redox polymer and the electrode. Prior to capturing the detection target, addition of H2O2 will lead to the highest reduction current due to the redox polymer-mediated, enzyme-amplified electroreduction of H2O2.
    Type: Grant
    Filed: April 28, 2021
    Date of Patent: October 10, 2023
    Assignee: MORGAN STATE UNIVERSITY
    Inventors: Yongchao Zhang, Aeshah Alshehri
  • Patent number: 11779962
    Abstract: The invention is a slide cleaner apparatus. The apparatus includes a cleaning chamber configured to receive a cell counting slide, at least one fluid inlet arranged to align with an inlet on a cell counting slide when located in the chamber, and configured to feed cleaning fluid into the cleaning chamber and slide, and at least one fluid outlet configured to remove cleaning fluid from the cleaning chamber and slide. The invention also includes methods of cleaning a cell counting slide.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: October 10, 2023
    Inventor: Ahmet Imrali
  • Patent number: 11760965
    Abstract: Mesofluidic devices for culturing cell aggregates and methods of using the same are disclosed. An exemplary mesofluidic device comprises at least one fluid inlet, at least one fluid outlet, a plurality of fluid channels, and a plurality of culture chambers. Each culture chamber can comprise at least one chamber inlet and at least one chamber outlet. The at least one chamber inlet can be in fluid communication with the at least one fluid inlet via at least one of the plurality of fluid channels. The at least one chamber outlet can be in fluid communication with the at least one fluid outlet via at least one of the plurality of fluid channels. The mesofluidic device can be configured to contain a cell aggregate in each of the plurality of culture chambers.
    Type: Grant
    Filed: October 19, 2018
    Date of Patent: September 19, 2023
    Assignees: Georgia Tech Research Corporation, Emory University
    Inventors: Emily Jackson-Holmes, Hang Lu, Zhexing Wen
  • Patent number: 11747244
    Abstract: A laboratory system and a method for separating interfering substances contained in test samples is presented. The laboratory system comprises separation vessels comprising solid surfaces and capturing molecules which are immobilized on the solid surfaces. The capturing molecules of the separation vessels are configured to bind interfering substances of laboratory tests of different analytical methods.
    Type: Grant
    Filed: April 16, 2019
    Date of Patent: September 5, 2023
    Assignee: ROCHE DIAGNOSTICS OPERATIONS, INC.
    Inventors: Michael Glauser, Dietmar Kappelhoff, Youssef Khayali
  • Patent number: 11724237
    Abstract: The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc.
    Type: Grant
    Filed: May 16, 2022
    Date of Patent: August 15, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Adam R. Abate, Tony Hung, Pascaline Mary
  • Patent number: 11712181
    Abstract: Presented herein are systems, methods for collecting fluid from a surface (e.g., skin) and analyzing the fluid (e.g., to measure chemical, physical and/or biological properties of the fluid). For example, a system for fluid collection on a surface (e.g., skin) and fluid analysis includes at least one of the following modules: (i) a collection and delivery module to collect a fluid over a wet or partially wet surface and deliver it to (ii) a main sensing module to perform chemical, physical and/or biological analysis on the fluid. The system also includes (iii) a flow regulation module, for controlling fluid flow (e.g., transport) through the system and (iv) a waste module to collect and/or dispose of the fluid after analysis is complete.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: August 1, 2023
    Assignee: Xsensio SA
    Inventors: Fabien Patrick Wildhaber, Neil Ebejer, Hoël Maxime Guérin, Johan Frédéric Longo
  • Patent number: 11708597
    Abstract: A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem including an actuation substrate, and a set of pins interacting with the actuation substrate, and a spring plate configured to bias at least one pin in a configurations, the valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; and a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: July 25, 2023
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Michael T. Kusner, Jeffrey Williams
  • Patent number: 11702688
    Abstract: An object of the present invention is to provide a novel method for designing a primer ensuring reactivity and discriminatory power in a method for detecting a single base substitution based on an ASP-PCR method and to provide a method for easily detecting multiple point mutations within overlapping amplicons, particularly, two adjacent single base substitutions. The single base substitutions can easily be detected by using a mutant primer in which the base of the third nucleotide from the 3? end corresponds to the base of a mutant nucleotide of a single base substitution contained in a nucleic acid sample, in which the base of the second nucleotide from the 3? end is not complementary to the base of the corresponding nucleotide of the nucleic acid, and in which the bases of the other nucleotides are complementary to the bases of the corresponding nucleotides of the nucleic acid.
    Type: Grant
    Filed: March 29, 2017
    Date of Patent: July 18, 2023
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Hiroyuki Ebinuma, Katsura Uchida, Yuriko Tsukamoto
  • Patent number: 11702650
    Abstract: A test device is provided that can comprise: a housing accommodating a chromatography support, wherein the housing comprises: a supporting part that supports a container accommodating a liquid used for chromatography. A method is provided for performing chromatography using the test device.
    Type: Grant
    Filed: May 8, 2020
    Date of Patent: July 18, 2023
    Assignee: KANEKA CORPORATION
    Inventors: Sotaro Sano, Shigehiko Miyamoto, Takaaki Jikihara
  • Patent number: 11703389
    Abstract: A spectrometry device includes a light source, an integrator configured to have an internal space in which a long afterglow emission material is disposed and output detection light from the internal space, a spectroscopic detector, an analysis unit configured to analyze a photoluminescence quantum yield of the long afterglow emission material, and a control unit configured to control switching between presence and absence of input of excitation light to the internal space and an exposure time in the spectroscopic detector. The control unit controls the light source so that the input of the excitation light to the internal space is maintained in a first period and the input of the excitation light to the internal space is stopped in a second period, and controls the spectroscopic detector so that an exposure time in the second period becomes longer than an exposure time in the first period.
    Type: Grant
    Filed: October 10, 2018
    Date of Patent: July 18, 2023
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventors: Kengo Suzuki, Shigeru Eura, Kazuya Iguchi
  • Patent number: 11697115
    Abstract: A dispensing device which has a dispensing channel extending between a supply opening and an output opening into the course of which a pump, a selection valve and a dispensing valve are connected. The selection valve is also connected to a pressurised air source. The dispensing device allows a dispensed output of a fluid, wherein a buffer channel portion is filled by the pump in a pump dispensing phase, to which buffer channel portion pressure is applied from the pressurised air source during a pressurised air dispensing phase such that a precise fluid output is subsequently possible by clocked actuation of the dispensing valve until the target fluid amount has been reached.
    Type: Grant
    Filed: September 13, 2018
    Date of Patent: July 11, 2023
    Assignee: FESTO SE & CO. KG
    Inventors: Bengt Erich Wunderlich, Bernhard Vogt, Duc-Thang Vu
  • Patent number: 11697814
    Abstract: The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Tumor Suppressor genes, in particular, by targeting natural antisense polynucleotides of Tumor Suppressor genes. The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of Tumor Suppressor genes.
    Type: Grant
    Filed: June 11, 2019
    Date of Patent: July 11, 2023
    Assignee: CuRNA, Inc.
    Inventors: Joseph Collard, Olga Khorkova Sherman
  • Patent number: 11692941
    Abstract: An optofluidic diagnostic system and methods for rapid analyte detections. The system comprises an optofluidic sensor array, a test plate and an optical detection cartridge. The sensor array supports one or more distinct sensor units, each having a reactor section designed to temporarily enter a series of different kinds of wells in the test plate. One kind of well is a sample reservoir that holds reagent solution to be transferred into the reactor section. Another kind of well is a drainage chamber that removes reagent solution from the reactor section. A third kind of well is a colorant reservoir that holds a colorant reagent transferable into a reactor section. Finally, the sensor unit is transferred to the optical detection cartridge where it is placed into an isolation booth during the optical detection process so that its flat observation face is stationed in a viewing window opposite an optical detector lens.
    Type: Grant
    Filed: October 25, 2021
    Date of Patent: July 4, 2023
    Assignee: Optofluidic Bioassay, LLC
    Inventors: Maung Kyaw Khaing Oo, Xudong Fan
  • Patent number: 11650204
    Abstract: Provided herein are systems and methods for performing assays. In particular, provided herein are systems and methods for performing sensitive and rapid immunoassays.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: May 16, 2023
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Young Geun Park, Byunghoon Ryu, Xiaogan Liang, Katsuo Kurabayashi
  • Patent number: 11649484
    Abstract: This invention relates to sequence specific electrochemically-labeled oligonucleotide probes for the detection of nucleic acids and methods associated therewith.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: May 16, 2023
    Assignee: ABBOTT DIAGNOSTICS SCARBOROUGH, INC.
    Inventors: Frank Ray Bowler, Grzegorz Artur Orlowski, Hazel Lucy Greetham, Cheng Zhou, Niall A. Armes, Olaf Piepenburg
  • Patent number: 11648551
    Abstract: Among other things, the present invention is related to devices and methods of performing biological and chemical assays, particularly an easy sample manipulation and/or a rapid change or a rapid thermal cycling of a sample temperature is needed (e.g. Polymerase Chain Reaction (PCR) for amplifying nucleic acids).
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: May 16, 2023
    Assignee: Essenlix Corporation
    Inventors: Stephen Y. Chou, Wei Ding, Ji Qi, Hua Tan, Yufan Zhang
  • Patent number: 11635428
    Abstract: According to one embodiment, a molecule detecting device includes a capturing section, a releasing section, and a detecting section. The capturing section is configured to, by combining a target molecule and a solubilizing agent with each other and thereby creating a composite body, capture the target molecule in a carrier liquid. The releasing section is configured to make the composite body release the target molecule therefrom in the carrier liquid. The detecting section is configured to carry out detection of the target molecule in the carrier liquid.
    Type: Grant
    Filed: September 10, 2020
    Date of Patent: April 25, 2023
    Assignee: Kabushiki Kaisha Toshiba
    Inventors: Hiroko Miki, Atsunobu Isobayashi, Yoshiaki Sugizaki
  • Patent number: 11612892
    Abstract: Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.
    Type: Grant
    Filed: December 2, 2020
    Date of Patent: March 28, 2023
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kevin D. Ness, Donald A. Masquelier, Billy W. Colston, Jr., Benjamin J. Hindson
  • Patent number: 11590491
    Abstract: An automatic liquid transfer optimization pipetting apparatus and method is disclosed. Namely, a liquid handling apparatus includes a pump supplying a nozzle (i.e., a pipette tip) via a conduit, one or more pressure sensors, and an electronic controller, and wherein the pipette tip is submerged in a liquid. Further, a method of automatic liquid transfer optimization pipetting includes the steps of actuating the pump to move a designated volume of liquid and then allowing the system to settle to a steady state after completion of pump actuation.
    Type: Grant
    Filed: December 27, 2018
    Date of Patent: February 28, 2023
    Assignee: FORMULATRIX, INC.
    Inventors: David Wikholm, Rasmus Lindblom
  • Patent number: 11587686
    Abstract: The present invention provides systems and methods for rapid screening of individuals who may have been exposed to and carrying biological agents potentially contagious or otherwise harmful to the general public.
    Type: Grant
    Filed: October 12, 2015
    Date of Patent: February 21, 2023
    Assignee: Cepheid
    Inventor: William E. Murray
  • Patent number: 11565268
    Abstract: The present invention discloses a convective PCR apparatus by using a transparent conductive thin film to replace the traditional metal heater. The PCR reaction is activated when the container with reagents contacted the heated transparent conductive thin film and the temperature inside the container raised to initiate the convective circulation. Also, the present invention could apply for a quantitative PCR reaction by adding a specific probe, a fluorescent dye, a light source, or a photon receiver.
    Type: Grant
    Filed: December 16, 2018
    Date of Patent: January 31, 2023
    Assignee: Credo Diagnostics Biomedical Pte. Ltd.
    Inventors: Ying-Ta Lai, Yu-Cheng Ou, Ming-Lung Hung, Cheng-Yueh Chung, Han-Yi Chen
  • Patent number: 11559803
    Abstract: The present invention describes an integrated apparatus that enables identification of migratory cells directly from a specimen. The apparatus only requires a small number of cells to perform an assay and includes novel topographic features which can reliably differentiate between migratory and non-migratory cell populations in a sample. Both the spontaneous and chemotactic migration of cancer cells may be measured to distinguish between subpopulations within a tumor sample. The migratory cells identified using the apparatus and methods of the present invention may be separated and further analyzed to distinguish factors promoting metastasis within the population. Cells in the apparatus can be treated with chemotherapeutic or other agents to determine drug strategies to most strongly inhibit migration. The use of optically transparent materials in some embodiments allows a wide range of imaging techniques to be used for in situ imaging of migratory and non-migratory cells in the apparatus.
    Type: Grant
    Filed: September 7, 2018
    Date of Patent: January 24, 2023
    Assignees: UNIVERSITY OF MARYLAND, BALTIMORE, THE JOHNS HOPKINS UNIVERSITY
    Inventors: Konstantinos Konstantopoulos, Colin Dowlin Paul, Alfredo Quinones-Hinojosa, Aikaterini Kontrogianni-Konstantopoulos
  • Patent number: 11548001
    Abstract: A system, apparatus, and method include a pump to deliver vapor including airborne contaminants including organic compounds including a target analyte; a collector to transfer the airborne contaminants by autonomous liquid extraction into a mobile organic liquid phase; a micro-fluidic chamber including immobilized biorecognition elements that bind to analytes delivered from the mobile organic liquid phase; a mechanism to introduce the mobile organic liquid phase to a buffer containing a plurality of substrates causing a series of biochemical reactions that create a change corresponding to a concentration of the target analyte; and a detector to perform real-time analysis that correlates to a concentration of the organic compounds to determine a presence of the target analyte.
    Type: Grant
    Filed: April 10, 2019
    Date of Patent: January 10, 2023
    Assignee: United States of America as represented by the Secretary of the Air Force
    Inventors: Mitchell Rubenstein, Douglas Adkins, Patrick Lewis, John Rogers, Sungbong Kim, Steve Kim, Michael Brothers
  • Patent number: 11549731
    Abstract: A heat pump that includes a thermoelectric device(s) and a heat sink having a raised portion with a top surface for thermally coupling with a planar face of the thermoelectric device(s). The raised portion of the heat sink includes an outer periphery and a raised central region surrounded by a void region to provide more uniform thermal conductivity when clamped within an assembly. The raised central region is shaped in an any shape corresponding to a shape of uneven thermal conductivity due to clamping pressure applied to the heat sink. The void region can be substantially contiguous and entirely circumscribe the central raised region. The device can optionally include discrete supports formed of a less thermally-conductive material within the void region. The supports can be elastomeric, such as O-rings, and disposed within pockets defined within the void region.
    Type: Grant
    Filed: January 2, 2020
    Date of Patent: January 10, 2023
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Amir Sadri, Nenad Kircanski, Thanh-Vi Tran, Carl Marlowe, Brian David Wilson
  • Patent number: 11549092
    Abstract: A cell evaluation device includes: a porous membrane having a first main face and a second main face; a first passage having a first passage portion facing a first area on which cells are placed in the first main face of the porous membrane; a second passage having a second passage portion facing a second area in the second main face of the porous membrane, the second area being positioned backside of the first area; and a first electrode provided in the first passage portion and a second electrode provided in the second passage portion, the first electrode and the second electrode being positioned across the first area and the second area. In the cell evaluation device, tight junctions are formed among the cells by cell cultivation. With the cell evaluation device, any increase in the electric resistance occurring due to the formation of the tight junctions can be easily measured.
    Type: Grant
    Filed: November 20, 2019
    Date of Patent: January 10, 2023
    Assignees: Shimadzu Corporation, Tokyo Institute of Technology
    Inventors: Yoichi Fujiyama, Chikara Miyake, Yoh-ichi Tagawa
  • Patent number: 11542546
    Abstract: The inventions cover systems and methods for generation of emulsions having suitable clarity without requiring refractive index matching between emulsion components. Systems can include: a substrate including a set of openings; a reservoir facing the substrate at a first side and containing a sample fluid configured for droplet formation upon interacting with the set of openings of the substrate; and a collecting container facing the substrate at a second side and containing a set of fluid layers configured with a density gradient and suitable immiscibility characteristics. One or more components of the system(s) can support methods for emulsion generation, in relation to enabling interactions between multiple continuous phases and a dispersed droplet phase to generate clear emulsions. Applications of the inventions(s) can include performance of droplet-based digital PCR in an improved manner (e.g., without requiring implementation of correction factors based upon Poisson statistics).
    Type: Grant
    Filed: February 16, 2022
    Date of Patent: January 3, 2023
    Assignee: Enumerix, Inc.
    Inventors: Hei Mun Christina Fan, Eleen Yee Lam Shum, Janice Hoiyi Lai, Stephen P. A. Fodor
  • Patent number: 11541395
    Abstract: A system for sex sorting of mosquitoes generally comprises: (i) a plate or similar receptacle that includes multiple wells, with each of the multiple wells configured to receive a mosquito; and (ii) an imaging subsystem, including a camera for capturing images of the mosquito in each of the multiple wells and a computer for analyzing the captured images to determine whether each mosquito is male or female. Mosquitoes have distinct anatomical areas for identification, which are captured in the images and are used to determine if a mosquito is male or female. Furthermore, in some implementations, the system includes a suction device or other means for exterminating the unwanted mosquitoes after the determination of whether each mosquito is male or female.
    Type: Grant
    Filed: August 21, 2019
    Date of Patent: January 3, 2023
    Assignee: MosquitoMate, Inc.
    Inventors: Stephen L. Dobson, James William Mains, Jim L. Peyton, Hakim Sultanov
  • Patent number: 11525160
    Abstract: Systems and methods for detecting at least two genomic alleles associated with corneal dystrophy in a sample from a human subject are disclosed in which cells (e.g., epithelial) of the subject are adhered to a tip of a substrate. The tip of the substrate is agitated in a lysis solution that lyses cells adhered to the substrate. The substrate is removed from the lysis solution upon completion of this agitation. The resulting lysis solution is incubated and then genomic DNA from the lysis solution is isolated to form a gDNA solution. From this, identity of at least two nucleotides present in the human TGF?I gene is determined using at least two oligonucleotide primer pairs and the gDNA solution. These at least two nucleotides are located at respective independent positions of the TGF?I gene corresponding to respective independent single nucleotide polymorphisms (SNPs) associated with corneal dystrophy.
    Type: Grant
    Filed: May 13, 2016
    Date of Patent: December 13, 2022
    Assignee: Avellino Lab USA, Inc.
    Inventors: Connie Chao-Shern, Sun-Young Cho, Gene Lee
  • Patent number: 11519030
    Abstract: The invention relates to a process and a device for analysing single molecules, particularly to the parallel analysis of a plurality of single molecules. It is suitable for detecting interactions, e.g. binding between single molecules and/or reactions, e.g. elongation or degradation of single molecules. Particularly, the process of the invention relates to the sequencing of single nucleic acid molecules. The single molecule to be analysed is present in free form, i.e. dissolved or suspended in a liquid medium, within a reaction space formed around the sample spot. According to the present invention, an electrical field is applied across the reaction space, whereby a concentration of single molecules, at the sample spots is effected.
    Type: Grant
    Filed: June 28, 2016
    Date of Patent: December 6, 2022
    Assignee: Gnothis Holding AG
    Inventor: Rudolf Rigler
  • Patent number: 11504690
    Abstract: An integrated circuit includes two or more rows of heating elements, two or more columns of heating elements, and a plurality of sensing areas. Each sensing area is between two adjacent rows of the rows of heating elements and between two adjacent columns of the columns of heating elements and includes a bio-sensing device and a temperature-sensing device.
    Type: Grant
    Filed: November 15, 2019
    Date of Patent: November 22, 2022
    Assignee: TAIWAN SEMICONDUCTOR MANUFACTURING COMPANY, LTD.
    Inventors: Tung-Tsun Chen, Yi-Shao Liu, Jui-Cheng Huang, Chin-Hua Wen, Felix Ying-Kit Tsui, Yung-Chow Peng
  • Patent number: 11504681
    Abstract: A microfluidic mixing device comprising two bellows pumps (105, 115), microfluidic cartridges comprising the same and methods for use of the same are provided. The disclosed device enables efficient mixing of samples at the microfluidic scale.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: November 22, 2022
    Assignee: PERKINELMER HEALTH SCIENCES, INC.
    Inventors: Andrew Kolb, Matthew Scott Bragd, C. Frederick Battrell
  • Patent number: 11506659
    Abstract: A lateral flow assay (LFA) device includes a capillary pad and a sample port that holds the sample fluid before a hole is made in a cavity surface of the sample port. The LFA device includes a breaker with a tip to make a hole in the cavity wall of the sample port causing the sample fluid held inside the compartment to be applied to the capillary pad after the start of a test.
    Type: Grant
    Filed: January 11, 2022
    Date of Patent: November 22, 2022
    Inventors: Mehdi Hatamian, Mehrtash Ghalebi
  • Patent number: 11505825
    Abstract: The disclosure provides methods of synthesizing DNA using topoisomerase-mediated ligation, by adding single nucleotides or oligomers to a DNA strand in the 3? to 5? direction.
    Type: Grant
    Filed: April 16, 2021
    Date of Patent: November 22, 2022
    Assignee: IRIDIA, INC.
    Inventor: Paul F. Predki