Measuring Or Testing For Antibody Or Nucleic Acid, Or Measuring Or Testing Using Antibody Or Nucleic Acid Patents (Class 435/287.2)
  • Patent number: 10737263
    Abstract: A method of manufacturing a device useable in DNA or genome sequencing comprises disposing pairs of electrodes on a substrate, the electrodes within each pair separated by a nanogap; depositing a resist layer over the electrodes; patterning the resist layer to create an exposed region on each electrode at or near each nanogap; roughening the electrode surface within each exposed region using various methods; and exposing the exposed regions to biomolecules, wherein one biomolecule bridges each nanogap of each electrode pair, with each end of each biomolecule bound to the electrodes at each exposed region.
    Type: Grant
    Filed: February 9, 2017
    Date of Patent: August 11, 2020
    Assignee: Roswell Biotechnologies, Inc.
    Inventors: Chulmin Choi, Sungho Jin, Paul W. Mola, Barry L. Merriman
  • Patent number: 10722888
    Abstract: Devices having nanochannels suitable for confinement and alignment of DNA molecules, as well as methods of fabricating the same and methods of using the same for DNA analysis, are provided. A device can include a dynamically-controlled, unified microchannel-nanochannel platform suitable for confinement and alignment of DNA molecules. The nanochannels can be reversibly formed within nanoslits formed in a deformable substrate or base layer.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: July 28, 2020
    Assignee: The Hong Kong University of Science and Technology
    Inventors: Shuhuai Yao, Miao Yu, Youmin Hou
  • Patent number: 10710075
    Abstract: Methods and devices for concentrating target cells using dielectrophoresis (DEP) are disclosed. The method allows relatively high throughput of sample through a microfluidic device in order to allow rapid capture of target cells even when they are present in low concentrations within the sample. The method utilizes multiple chambers through which samples will flow, the chambers arranged such that the first capture area has a larger area and faster flow rate than a second chamber, the second chamber being positioned downstream of the first capture area and being smaller with a slower flow rate to further concentrate the material captured in the first capture area.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: July 14, 2020
    Assignee: QUANTUMDX GROUP LIMITED
    Inventors: Jonathan O'Halloran, Heather Murton, Stephen Osborne, Eduardo Boada, Jonathan Salmon
  • Patent number: 10704042
    Abstract: Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.
    Type: Grant
    Filed: April 30, 2018
    Date of Patent: July 7, 2020
    Assignee: GE HEALTHCARE BIO-SCIENCES CORP.
    Inventors: John R. Nelson, R. Scott Duthie, Rohini Dhulipala, Gregory A. Grossman, Anuradha Sekher
  • Patent number: 10694984
    Abstract: There is provided a test method for analysing a body fluid in which a test tape is used in a test device to successively provide analytical test fields stored on the test tape, wherein body fluid is applied by a user to the test field provided at a time and the said test field is photometrically scanned using a measuring unit of the device to record measurement signals. To increase the measurement reliability, it is proposed that a control value is determined from a time-dependent and/or wavelength-dependent change in the measurement signals and that the measurement signals are processed as valid or discarded as erroneous depending on the control value.
    Type: Grant
    Filed: February 18, 2019
    Date of Patent: June 30, 2020
    Assignee: Roche Diabetes Care, Inc.
    Inventors: Karl Miltner, Robert Lorenz, Ulrich Porsch, Clemens Knapp
  • Patent number: 10646869
    Abstract: (1) Plural reaction chambers each including at least one cell capturing unit and at least one nucleic acid capturing unit connected via a flow channel, (2) a first liquid flow channel commonly connected to the plural reaction chambers on a first surface side, (3) plural second liquid flow channels connected to the plural reaction chambers in a one-on-one relationship on the first surface side, and (4) a third liquid flow channel commonly connected to the plural reaction chambers on a second surface side opposite to the first surface, are provided in a flow cell device for single cell analysis.
    Type: Grant
    Filed: February 3, 2015
    Date of Patent: May 12, 2020
    Assignee: Hitachi, Ltd.
    Inventors: Masataka Shirai, Tomoyuki Sakai
  • Patent number: 10639633
    Abstract: A controller is connected to controlled devices on a microfluidic chip with a multiplexer. Signal transmission band width is differently allocated amongst the plurality of controlled devices on the microfluidic chip.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: May 5, 2020
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Sirena C. Lu, Manish Giri, Matthew David Smith, Melinda M. Valencia
  • Patent number: 10626432
    Abstract: A composition is provided. The composition comprises an enzyme and a ruthenium- or osmium-based electron transfer agent, wherein the ruthenium- or osmium-based electron transfer agent is a complex [M(A)x(B)y](X)n wherein M is ruthenium or osmium; A is an amine ligand; each B is a ligand different to A; x is an integer selected from 1 to 5; y is an integer selected from 1 to 5; x+y is 6 or 8; n is an integer selected from 1 to 6; and X is any suitable counterion.
    Type: Grant
    Filed: April 28, 2016
    Date of Patent: April 21, 2020
    Assignee: INSIDE BIOMETRICS INTERNATIONAL LIMITED
    Inventors: Marco Cardosi, Stephanie Kirkwood
  • Patent number: 10626454
    Abstract: The present disclosure provides compositions and methods for nucleic acid amplification and analysis. In some embodiments, a nucleic acid amplification reaction mixture is formulated to minimize or eliminate the inclusion of a species that can affect activity of one or more inhibitory species in the nucleic acid amplification reaction mixture. Such reaction mixtures can permit suitable analysis of nucleic acid sample prior to their use in one or more downstream assays, including nucleic acid sequencing assays.
    Type: Grant
    Filed: July 26, 2017
    Date of Patent: April 21, 2020
    Assignee: SeqMatic, LLC
    Inventors: Victor Hum, Danny Lee, Kelvin Chan
  • Patent number: 10627336
    Abstract: An optical measurement device with a transparent cylindrical measurement cuvette, which defines an axial symmetrical axis and contains the fluid sample, an optical measurement arrangement which quantitatively determines the optical property of the fluid sample in the measurement cuvette, and a mechanical cleaning arrangement is described. The cleaning arrangement comprises an external magnetic working platform, which is arranged coaxially to the symmetrical axis, is slidable in a translatory manner parallel to the symmetrical axis, is rotatable about the symmetrical axis, encloses the cylindrical measurement cuvette externally in an annular manner, and has at least one translatory magnet element and at least one rotary magnet element, and an internal magnetic cleaning unit, which is arranged within the measurement cuvette and is slidable in a translatory manner, has a cleaning body, has at least one translatory magnet element, and has at least one rotary magnet element.
    Type: Grant
    Filed: November 7, 2017
    Date of Patent: April 21, 2020
    Assignee: HACH LANGE GMBH
    Inventors: Frank Steinhauer, Roman Klein, Robert Dalljo
  • Patent number: 10619192
    Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.
    Type: Grant
    Filed: April 8, 2015
    Date of Patent: April 14, 2020
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Daniel T. Chiu, Jason E. Kreutz, Gloria S. Yen, Bryant S. Fujimoto
  • Patent number: 10618931
    Abstract: Provided herein are methods for the synthesis of oligomeric compounds wherein removal of the 5?-terminal trityl group is performed at reduced temperature and lower pH relative to standard methods. In certain embodiments, the present methods provide detritylated oligomeric compounds having a reduced percentage of depurination relative to the same detritylated oligomeric compounds prepared using standard methods. In certain embodiments, the present methods provide detritylated oligomeric compounds with increased purity relative to the same detritylated oligomeric compounds prepared using standard methods. In certain embodiments, the present methods provide an increased rate of detritylation compared to standard methods.
    Type: Grant
    Filed: July 27, 2017
    Date of Patent: April 14, 2020
    Assignee: Ionis Pharmaceuticals, Inc.
    Inventor: Dana Chreng
  • Patent number: 10619202
    Abstract: A device (100) and a method optically control a chemical reaction in a reaction chamber (149) holding a reagent fluid (114). The chemical reaction includes a nucleic acid sequencing on a wiregrid. Based on strong optical confinement of excitation light (110) and of cleavage light (112), the sequencing reaction can be read-out. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moieties. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked. In order to avoid overheating by cleavage light, the reagent fluid is circulated along the surface of the substrate (101).
    Type: Grant
    Filed: November 20, 2014
    Date of Patent: April 14, 2020
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Pieter Jan Van Der Zaag, Anke Pierik
  • Patent number: 10620110
    Abstract: A sample analyzer with an optical detection device and a sample analysis method of the sample analyzer are disclosed. The optical detection device includes a fluid chamber, a light source and a light detector. The fluid chamber includes an illumination zone. An analyte flows through the illumination zone so as to form a sample stream. The light source illuminates the illumination zone to excite cell articles, reacted with a reagent, of the sample stream to emit a light signal. The light detector detects the fluorescent lights and transforms it into an electric signal. The light detector can include a silicon photomultiplier.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: April 14, 2020
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Xiansuan Du, Yong Dai
  • Patent number: 10620190
    Abstract: An automated microscope apparatus, comprises an outer housing having an external wall; optionally but preferably an internal wall in said housing, and configured to form a first compartment and a separate second compartment in said outer housing; a microscope assembly in said housing, preferably in said first compartment; and a microprocessor in said housing, preferably in said second compartment; and optionally but preferably a heat sink mounted on said housing external wall, preferably adjacent said second compartment, with said microprocessor thermally coupled to said heat sink and operatively associated with said microscope assembly.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: April 14, 2020
    Assignee: Advanced Animal Diagnostics, Inc.
    Inventors: Stefano Bresolin, David A. Calderwood, Tobias M. Heineck, David Newcomb, Chris Paul, Jaspar N> Pollard, Rodolfo R. Rodriguez, Demetris Young
  • Patent number: 10613256
    Abstract: A biometric device includes an illuminating unit and an imaging module. The imaging module includes an optical angular selective structure and a sensing layer. The light selecting structure includes a micro lens array, a refractive layer and a light shielding layer. The refractive layer is disposed between the micro lens array and the light shielding layer. The micro lens array includes a plurality of lens unit units, and the light shielding layer has a plurality of light passing portions. The sensing layer defines multiple sensing regions which are spaced apart from each other. The light shielding layer is disposed between the refractive layer and the sensing layer. The sensing regions correspond to the light passing portions, respectively. An optical angular selective distance is defined between the light shielding layer and the sensing layer.
    Type: Grant
    Filed: December 27, 2017
    Date of Patent: April 7, 2020
    Assignee: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
    Inventors: Ding-Zheng Lin, Yu-Ling Hsu, Yaw-Ting Wu, Ping-Chen Chen
  • Patent number: 10603742
    Abstract: An additive manufacturing temperature controller/temperature sensor uses one or more spectrophotometric sensors to monitor temperature of successive layers and preferably localized sections of successive layers of a melt pool, and transients thereof, of an object being generated for the purpose of dynamic control of the additive manufacturing device and/or quality control of the generated object manufactured with the additive manufacturing device. Generally, the additive manufacturing temperature controller/sensor apparatus monitors temperature of a section of the object during manufacture as a function of wavelength, time, position, and/or angle to determine melt extent in terms of radius and/or depth.
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: March 31, 2020
    Inventor: James Eldon Craig
  • Patent number: 10604796
    Abstract: The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: March 31, 2020
    Assignee: Applied Biosystems, LLC
    Inventors: Shoulian Dong, Junko F. Stevens, Danny H. Lee
  • Patent number: 10598657
    Abstract: Test devices are provided for determining the presence of a first ligand in a sample. In some embodiments depletion conjugates are used to deplete the ligands different from but related to the first ligands from the sample. In some embodiments, interim binding agents are used to enhance the test signal.
    Type: Grant
    Filed: April 17, 2018
    Date of Patent: March 24, 2020
    Assignee: Chembio Diagnostic Systems, Inc.
    Inventor: Javanbakhsh Esfandiari
  • Patent number: 10591469
    Abstract: The present invention is intended to provide an immunoassay method that enables an immunoassay with high sensitivity at a high development rate without causing aggregation of insoluble carriers or non-specific reaction while improving test efficiency and reducing labor. The present invention relates to an immunoassay method that uses a test device, and the method includes: extracting an antigen of a detection target in an analyte with an extraction agent; and detecting the detection target with a detection reagent capable of binding the antigen. The extraction agent is a nitrous acid generated on the test device by a contact reaction between a nitrite salt and a heterocyclic compound having at least one skeleton selected from the group consisting of a cyclic ester, a cyclic amide, and a cyclic imide.
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: March 17, 2020
    Assignee: TANAKA KIKINZOKU KOGYO K.K.
    Inventors: Yuya Kato, Hisahiko Iwamoto
  • Patent number: 10590484
    Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: March 17, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Jonas Korlach, Stephen Turner, Tyson A. Clark
  • Patent number: 10545136
    Abstract: The present invention relates to methods for prognosis and monitoring of a cancer, preferably bladder cancer, by measuring the proportion of CD8+ T Lymphocytes expressing ILT-2 and the plasma level of soluble HLA-G. The invention also relates to anti HLA-G antibodies for use for treating a cancer in which tumor cells express HLA-G, preferably bladder cancer.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: January 28, 2020
    Assignees: ASSISTANCE PUBLIQUE HOPITAUX DE PARIS, COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, Université Paris Diderot—Paris 7
    Inventors: François Desgrandchamps, Edgardo Delfino Carosella, Joël Lemaoult
  • Patent number: 10545083
    Abstract: We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.
    Type: Grant
    Filed: August 7, 2015
    Date of Patent: January 28, 2020
    Assignee: Sphere Fluidics Limited
    Inventors: Clive Adrian Smith, Xin Li, Frank F. Craig
  • Patent number: 10538808
    Abstract: Disclosed herein are compositions, probes, devices, and processes useful for detecting specific reactions and binding interactions with biological molecules. In certain embodiments, methods of binding one or more biomolecules to a solid support are disclosed. Methods of generating site-specific sequences for one or more biomolecules from a solid support are also disclosed. Biological complexes generated by these methods are also disclosed.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: January 21, 2020
    Assignee: Vibrant Holdings, LLC
    Inventor: John J. Rajasekaran
  • Patent number: 10539461
    Abstract: A spectrometer includes a support having a bottom wall part in which a depression including a concave curved inner surface and a peripheral part adjacent to the depression are provided, and a side wall part disposed on a side on which the depression is open with respect to the bottom wall part, a light detection element supported by the side wall part while opposing the depression, and a dispersive part disposed on the inner surface of the depression. A length of the depression in a second direction in which a plurality of grating grooves included in the dispersive part is aligned is larger than a length of the depression in a third direction orthogonal to the second direction when viewed in a first direction in which the depression and the light detection element oppose each other. An area of the peripheral part adjacent to the depression in the second direction is larger than an area of the peripheral part adjacent to the depression in the third direction when viewed in the first direction.
    Type: Grant
    Filed: March 5, 2019
    Date of Patent: January 21, 2020
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventors: Takafumi Yokino, Katsumi Shibayama, Katsuhiko Kato
  • Patent number: 10533994
    Abstract: This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.
    Type: Grant
    Filed: September 28, 2015
    Date of Patent: January 14, 2020
    Assignee: Theranos IP Company, LLC
    Inventors: Elizabeth A. Holmes, Shaunak Roy, Ian Gibbons, Shize Daniel Qi, Edmond Ku, Chris Todd, Melissa Takahashi, Bruce Johnson, Jeff Fenton, Keith Moravick, Uwe Springborn, Anthony Delacruz
  • Patent number: 10532109
    Abstract: Provided herein are therapeutic agents having specificity for having inhibitory activity against cancer cells that overexpress human epidermal growth factor receptor (HER) genes, including therapeutic agents comprising one or more HER-targeting peptides, pharmaceutical compositions comprising such therapeutic agents, and methods of using such compositions to treat or prevent a cancer or other disease condition associated with HER overexpression.
    Type: Grant
    Filed: May 8, 2017
    Date of Patent: January 14, 2020
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Shengxi Chen, Yanmin Zhang, Sidney Hecht
  • Patent number: 10527761
    Abstract: In one aspect, coated optical elements are described herein. In some implementations, a coated optical element comprises an optical element and a graphene coating layer disposed on a surface of the optical element. The graphene coating layer, in some implementations, has a thickness of about 100 nm or less.
    Type: Grant
    Filed: January 14, 2013
    Date of Patent: January 7, 2020
    Assignee: The Boeing Company
    Inventors: Angela W Li, Jeffrey H Hunt, Wayne R. Howe
  • Patent number: 10525077
    Abstract: The present invention provides an oligodeoxy nucleotide for preparing drugs for inhibiting tumor growth and an application thereof. The core sequence of the oligodeoxy nucleotide is TTYSGGAAWT, wherein Y is C or T; S is C or G; and W is A or T. The oligodeoxy nucleotide above further comprises an antisense strand and a modified type thereof. The oligodeoxy nucleotide according to the present invention plays a role of inhibiting the tumor growth in vitro and vivo, and is expected to be used for preparing drugs for inhibiting tumor growth, with high specificity and high inhibition ratio.
    Type: Grant
    Filed: June 25, 2015
    Date of Patent: January 7, 2020
    Assignees: JIANGSU KEYGEN BIOTECH CORP., LTD, NANJING NEW INDUSTRY INVESTMENT GROUP CO., LTD
    Inventors: Xuegen Wang, Qing Ye, Yijun Sun
  • Patent number: 10524764
    Abstract: An ultrasonic device includes a base, a plurality of ultrasonic transducer elements, and a reinforcing body. The base defines a plurality of openings arranged in an array form. The ultrasonic transducer elements are arranged respectively corresponding to the openings with a plurality of vibration films being respectively provided for the ultrasonic transducer elements. The reinforcing body is fixed to the base in an area between adjacent ones of the vibration films when viewed in a plan view along a thickness direction of the base. The reinforcing body has Young's modulus greater than Young's modulus of the base.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: January 7, 2020
    Assignee: Seiko Epson Corporation
    Inventors: Kanechika Kiyose, Hironori Suzuki, Hiroshi Matsuda
  • Patent number: 10501791
    Abstract: A method of sequencing nucleic acids is provided using sequencing by ligation and/or sequencing by hybridization.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: December 10, 2019
    Inventors: George M. Church, Richard C. Terry, Frederic Vigneault, Francois Vigneault
  • Patent number: 10495656
    Abstract: This invention provides biochip cartridges and instrument devices for the detection and/or analysis of target analytes from patient samples.
    Type: Grant
    Filed: July 23, 2015
    Date of Patent: December 3, 2019
    Assignee: GenMark Diagnostics, Inc.
    Inventors: Jon Faiz Kayyem, Jayashankar Srinivasan, Sean Ford
  • Patent number: 10487350
    Abstract: The present invention provides an in vitro method for concentrating infectious pathogens found in a biological sample obtained from an individual who is suspected of being infected with the pathogens. Provided herein is also an in vitro method for reducing or eliminating blood cells from a sample obtained from an individual suspected to being infected with an infectious pathogen. The present invention also provides a method for diagnosing malaria and a method for determining if an individual is infected with a pathogen. Provided herein is also a concentrator and a kit for use with the methods.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: November 26, 2019
    Assignee: ExThera Medical Corporation
    Inventors: Robert S. Ward, Keith R. McCrea
  • Patent number: 10481077
    Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
    Type: Grant
    Filed: August 8, 2019
    Date of Patent: November 19, 2019
    Assignee: Celsee Diagnostics, Inc.
    Inventor: Kalyan Handique
  • Patent number: 10466194
    Abstract: One embodiment is a biochip having an array of biosensors for quantitative determination of protein-protein and/or antibody-antigen interactions. The array comprises at least two biosensors formed on a substrate. The biosensors can be used to detect either the same or different analytes. The biosensors may be acoustical transducers operated in the thickness shear mode of vibration, wherein the density, viscosity, and elasticity at the sensor interface can be ascertained. Additionally, a series of fluidic channels are etched to the same depth as the biosensors. The fluidic channels serve to efficiently deliver the sample to the biosensors. One or more biochips can be housed within an enclosure.
    Type: Grant
    Filed: February 11, 2016
    Date of Patent: November 5, 2019
    Assignee: Drexel University
    Inventors: Johann deSa, Qiliang Zhang, Ryszard Lec, Ertan Ergezen
  • Patent number: 10460140
    Abstract: A method for tracking an item that includes the steps of (a) providing an item having a solid polymer material; (b) irradiating the item to create a spatial pattern of optical modifications in the polymer material, wherein the spatial pattern of optical modifications includes a code that indicates information; and (c) detecting the pattern of optical modifications, thereby determining the information.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: October 29, 2019
    Assignee: ILLUMINA, INC.
    Inventors: Dustin Blair, Nitin Udpa, Linh Tran, Nicholas Scheidler
  • Patent number: 10457975
    Abstract: Provided herein is a method and a system for separating suspended particles that have differing sedimentation velocities, comprising placing the particles inside a hollow device which upon rotation, spins the suspension into a thick film within which sedimentation of the particles occurs, rotating the hollow device at a speed and for a time that allows fast-sedimenting particles to sediment into a layer of more densely packed particles while some of the slow-sedimenting particles remain outside of the dense-particle-pack, and slowing the rotation of the hollow device in a manner to prevent remixing of the layer of dense-particle-pack with a layer containing some of the slow-sedimenting particles. This method may have application in many technologies including the rapid separation of bacteria from blood components.
    Type: Grant
    Filed: February 9, 2017
    Date of Patent: October 29, 2019
    Inventors: William George Pitt, Daniel S. McClellan, Colin G. Bledsoe, Mahsa Alizadeh
  • Patent number: 10457974
    Abstract: The present invention provides an in vitro method for concentrating infectious pathogens found in a biological sample obtained from an individual who is suspected of being infected with the pathogens. Provided herein is also an in vitro method for reducing or eliminating blood cells from a sample obtained from an individual suspected to being infected with an infectious pathogen. The present invention also provides a method for diagnosing malaria and a method for determining if an individual is infected with a pathogen. Provided herein is also a concentrator and a kit for use with the methods.
    Type: Grant
    Filed: November 2, 2016
    Date of Patent: October 29, 2019
    Assignee: ExThera Medical Corporation
    Inventors: Robert S. Ward, Keith R. McCrea
  • Patent number: 10429292
    Abstract: For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: October 1, 2019
    Assignee: Iris International, Inc.
    Inventors: Thomas H. Adams, Bart J. Wanders, John Roche, Harvey L. Kasdan
  • Patent number: 10421070
    Abstract: Embodiments of the present invention relate to methods and apparatuses for the discretization and manipulation of sample volumes that is simple, robust, and versatile. It is a fluidic device that partitions a sample by exploiting the interplay between fluidic forces, interfacial tension, channel geometry, and the final stability of the formed droplet and/or discretized volume. These compartmentalized volumes allow for isolation of samples and partitioning into a localized array that can subsequently be manipulated and analyzed.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: September 24, 2019
    Assignee: UNIVERSITY OF WASHINGTON
    Inventors: Daniel T. Chiu, Dawn E. Cohen, Gavin D. M. Jeffries
  • Patent number: 10391467
    Abstract: Provided herein are methods and compositions for the fabrication of patterned arrays, such as nucleotide arrays. The methods and compositions are suited for the transfer and reorientation of array components.
    Type: Grant
    Filed: December 5, 2014
    Date of Patent: August 27, 2019
    Assignee: CENTRILLION TECHNOLOGY HOLDINGS CORPORATION
    Inventors: Wei Zhou, Filip Crnogorac, Glenn McGall, Jian Cao
  • Patent number: 10393759
    Abstract: The present invention, in some embodiments, generally relates to methods of determining a treatment protocol for and/or a prognosis of a patient's recovery from a brain injury. In some embodiments, the brain injury results from a hypoxic event. In some embodiments, methods are provided for determining a measure of the concentration of tau protein in a patient sample containing or suspected of containing tau protein.
    Type: Grant
    Filed: September 19, 2016
    Date of Patent: August 27, 2019
    Assignee: Quanterix Corporation
    Inventors: David Wilson, John Henrik Zetterberg, Kaj Blennow, Jeffrey D. Randall
  • Patent number: 10385401
    Abstract: An object of the invention is an in vitro method for detecting chromosomal rearrangements between at least two specific chromosomal regions in a biological sample of a human subject, which comprises the steps of: a) isolating deoxyribonucleic acid (DNA) molecules comprising said specific chromosomal regions from said biological sample, wherein said DNA molecules have an average length of X base pairs; b)amplifying the DNA molecules of step a) by a multiplex polymerase chain reaction assay, said assay comprising at least two sets of primers, wherein each set of primers is capable of hybridizing with a specific reference chromosomal region, and each set of primer comprises a plurality of primers, said primers being capable of hybridizing to a nucleic acid strand of one of the said specific chromosomal regions at sites regularly spaced of less than X/2 base pairs; and hybridizing the product of the amplification of step b) with at least one set of nucleic probes.
    Type: Grant
    Filed: November 21, 2014
    Date of Patent: August 20, 2019
    Assignee: Assistance Publique Hopitaux de Paris
    Inventors: Raphael Saffroy, Antoinette Lemoine
  • Patent number: 10374115
    Abstract: A microfluidic system includes a liquid drop accommodation space, an array of photosensitivity detection circuits and an array of driving circuits between an upper substrate and a lower substrate. Each photosensitivity detection circuit includes a photosensitive transistor and a first gating transistor. The photosensitive transistor has a gate electrode coupled to a first scan signal line, a source electrode coupled to a first power supply voltage signal line, and a drain electrode coupled to a source electrode of the first gating transistor. The first gating transistor has a gate electrode coupled to a second scan signal line, and a drain electrode coupled to a read signal line. Each driving circuit includes a driving transistor and a driving electrode. The driving transistor has a gate electrode coupled to a third scan signal line, a source electrode coupled to a data signal line, and a drain electrode coupled to the driving electrode.
    Type: Grant
    Filed: May 4, 2018
    Date of Patent: August 6, 2019
    Assignee: BOE TECHNOLOGY GROUP CO., LTD.
    Inventors: Xueyou Cao, Xue Dong, Haisheng Wang, Xiaoliang Ding, Yingming Liu, Yanling Han, Yuzhen Guo, Pengpeng Wang, Chihjen Cheng, Ping Zhang, Wei Liu, Likai Deng, Yangbing Li
  • Patent number: 10364460
    Abstract: A reaction container includes a base material, an analysis well in which a nucleic acid analysis reagent used for a nucleic acid analysis is positioned, the analysis well being arranged on the base material and configured to analyze a sample containing a nucleic acid, and a quantification well in which a quantification reagent configured to specifically detect the nucleic acid is positioned, the quantification well being arranged on the base material and configured to quantify an amount of the nucleic acid contained in the sample, in which the nucleic acid contained in the sample is distributed into the quantification well and the analysis well.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: July 30, 2019
    Assignee: TOPPAN PRINTING CO., LTD.
    Inventors: Masayuki Ogino, Tomohiko Azuma, Kenichi Sakane
  • Patent number: 10349589
    Abstract: A point-of-care diagnostic system that includes a cartridge and a reader. The cartridge can contain a patient sample, such as a blood sample. The cartridge is inserted into the reader and the patient sample is analyzed. The reader contains various analysis systems, such as an electrophoresis detection system that uses electrophoresis testing to identify and quantify various components of the blood sample. The reader can process data from the various patient sample analysis to provide interpretative results indicative of a disorder, condition, disease and/or infection of the patient.
    Type: Grant
    Filed: September 8, 2017
    Date of Patent: July 16, 2019
    Assignee: HEMEX HEALTH, INC.
    Inventors: Peter Galen, Umut Atakan Gurkan, Arwa Fraiwan, Muhammad Noman Hasan, Daniel E. Grupp, Joshua King Hoyt, James Thorne, Brian T. Grimberg
  • Patent number: 10334981
    Abstract: The present invention relates to a beverage preparation device (100) including a plurality of storage chambers for storing several ingredients having each a different taste property, a user interface (14) for receiving data representing a user's preferred taste, a taste management unit (10) and a generator (15). The taste management unit (10) includes a memory (11) for storing a reference table which describes the taste property of each ingredient and a processor (12) for determining the dosage of each ingredient to be used according to the reference table and the user's preferred taste. The generator (15) generates beverages from the several ingredients according to the determined dosage. The main advantage of this invention is that the beverage preparation device (100) can provide beverages according to the user's desired taste.
    Type: Grant
    Filed: November 28, 2011
    Date of Patent: July 2, 2019
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Jingwei Tan, Eng Cheng Khaw
  • Patent number: 10335791
    Abstract: Methods for detecting target analytes utilizing an array of wells are advantageous for detection of low concentrations of target analytes. Use of an array of wells requires sealing of the wells. The methods provided herein utilize digital microfluidics to seal wells of an array with a fluid that is immiscible with the aqueous liquid present in the wells to prevent evaporation and contamination of the aqueous fluid during analysis of signals from the wells. The disclosed method include generating a biphasic droplet composed of the immiscible fluid and an aqueous fluid. The immiscible fluid present in the biphasic droplet is moved over the array of wells to seal the wells by electrically actuating the aqueous fluid present in the biphasic droplet which in turn pulls the immiscible fluid.
    Type: Grant
    Filed: January 17, 2018
    Date of Patent: July 2, 2019
    Assignees: Abbott Laboratories, The Katholieke Universiteit Leuven
    Inventors: Mark A. Hayden, Karen Leirs, Elena Pérez-Ruiz, Jeroen Lammertyn
  • Patent number: 10315370
    Abstract: Method for producing a plastic molded part (1), wherein, in the method, a mold insert (3) with a diffractive surface relief (32) is provided, the mold insert (3) is inserted into one mold half of an injection mold (5) which, together with at least one further mold half, forms a cavity for producing the plastic molded part (1), wherein the mold insert (3) is inserted into the injection mold (5) such that the diffractive surface relief (32) forms a partial area of the surface of the cavity formed by the mold half (5), and the plastic molded part (1) is molded by injection molding by means of the injection mold (5). The invention furthermore relates to a mold insert as well as an injection mold for such a method, as well as a plastic molded part produced in this way.
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: June 11, 2019
    Assignees: LEONHARD KURZ STIFTUNG & CO. KG, OVD KINEGRAM AG
    Inventors: Harald Burger, Andreas Schilling, Volker Dinkelmeyer, Sebastian Mader
  • Patent number: 10317391
    Abstract: An electro-optical stimulation and recording system is disclosed, including a substrate and a plurality of wells coupled to the substrate. The system also includes at least one electrode set disposed proximate a respective one of the plurality of wells, wherein the electrode set comprises at least one electrode configured to collect an electric signal associated with at least a portion of the respective well. The system also includes a light-emitting element set corresponding to a respective one of the wells and configured to deliver optical stimulation to at least a portion of the respective well.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: June 11, 2019
    Assignee: Axion BioSystems, Inc.
    Inventors: Isaac Perry Clements, Amanda Jervis Preyer, Swaminathan Rajaraman, Daniel Christopher Millard, James David Ross