Abstract: The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.

Abstract: A nucleic acid amplifier may include a sample preparation zone, a fluid ejector, an amplification zone and a capillary break between the amplification zone and the fluid ejector.

Abstract: A quantum circuit generation method includes determining a reference state of a target molecule and N excitations states corresponding to the reference state, where N is a positive integer greater than or equal to 1; determining M excitations states from the N excitations states based on an attribute of the reference state and attributes of the N excitations states, where M is a positive integer greater than or equal to 1 and less than or equal to N; and generating a first quantum circuit based on the M excitations states such as to improve computation efficiency and to reduce resource consumption.

Abstract: Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Abstract: An electrode apparatus comprising: a substrate, the substrate comprising: a first microfluidic channel; a second microfluidic channel; and at least one conduit extending between the first microfluidic channel and the second microfluidic channel; wherein the first microfluidic channel and the at least one conduit comprise electrically conductive material, and wherein the second microfluidic channel is exposed to the external environment and configured to absorb liquid by capillary action.

Abstract: A field portable diagnostic apparatus uses a rotatable disk in which a microfluidic circuit is defined. The microfluidic circuit includes a centrifugal separation chamber receiving a sample to stratify the sample. A magnetic bead holding chamber is communicated to a mixing chamber, where mass amplifying functionalized magnetic-nanoparticles, held in a buffer solution and contained in the magnetic bead holding reservoir communicated to mixing chamber, are mixed with the separated fluid delivered to mixing chamber from the separation chamber. The functionalized magnetic nanoparticles conjugate with a target analyte in the sample. A magnet in proximity to a SAW chamber including a SAW detector draws the functionalized magnetic nanoparticles toward antibodies immobilized on the SAW sensor surface A wash reservoir is communicated to the SAW sensor chamber, and a cleanup/waste reservoir is communicated to the SAW chamber for receive fluid after it has passed through the SAW chamber.

Abstract: Disclosed herein are compositions, kits, and methods for treating a disorder of genomic imprinting in a subject. The method may include modifying a zinc-finger protein 274 (ZNF274) binding site on maternal chromosome 15 at position 15q11-q13 of the subject, such that the binding of a ZNF274 protein to the ZNF274 binding site is reduced relative to a control. The ZNF274 binding site comprises a polynucleotide having at least 90% sequence identify to SEQ ID NO: 1 or SEQ ID NO: 42. Further provided are DNA targeting systems that bind to a ZNF274 binding site or to a gene encoding a ZNF274 protein.

Abstract: The present disclosure provides an immunochromatographic test piece which prevents a non-specific reaction by efficiently and continuously contacting and neutralizing a developing solution containing nitrous acid with a neutralizing reagent in an immunochromatography method of extracting and measuring a sugar chain antigen by nitrous acid extraction on the immunochromatographic test piece. The present disclosure also provides an immunochromatographic test piece for extracting and measuring a sugar chain antigen in a specimen, which comprises: a sample pad; a label region; a detection region on which the antibody against the sugar chain antigen is immobilized; a region impregnated with a neutralizing reagent upstream of the label region, and a region impregnated with a solid acid reagent or a region impregnated with nitrite, upstream of the region impregnated with the neutralizing reagent.

Abstract: The present invention relates to an artificially manipulated immune system having an improved immune effect. More particularly, the present invention relates to an immune system having functions artificially altered which comprises artificially manipulated immunoregulatory elements and cells containing the same. Contemplated according to a particular embodiment is an immune system comprising artificially manipulated immunoregulatory genes such as PD-1, CTLA-4, A20, DGK?, DGK?, FAS, EGR2, PPP2R2D, PSGL-1, KDM6A, and TET2, and/or expression products thereof.

Abstract: Systems and methods for imaging and image-based analysis of test devices can include a background device for a lateral flow assay test strip. In one aspect, the background device can include a test strip portion sized and shaped to guide placement, on the background device, of the lateral flow assay test strip; a background portion at least partially surrounding the test strip portion; and one or more features of the background portion. The one or more features can include line detection fiducials, position fiducials, modulation transfer function fiducials, motion blur detection fiducials, and/or RGB balanced areas for evaluation of lighting conditions.

Abstract: A method of operating an integrated circuit includes using a first switching device to couple a bio-sensing device to a first signal path, generating, using the bio-sensing device, a bio-sensing signal on the first signal path in response to an electrical characteristic of a sensing film, using a second switching device to couple a temperature-sensing device to a second signal path, and generating, using the temperature-sensing device, a temperature-sensing signal on the second signal path in response to a temperature of the sensing film. The first and second switching devices, the bio-sensing device, the temperature-sensing device, and the sensing film are components of a sensing pixel of a plurality of sensing pixels of the integrated circuit.

Abstract: The present invention provides a fluidic device in which solutions of different concentrations can be easily obtained.

Abstract: The present invention is related to a method for recovering two or more genes, or gene products, or cDNAs, encoding for an immunoreceptor having two or more subunits, which two or more genes, or gene products, are comprised in a given source cell. The invention is further related to a method of creating a library of expressor cells, in which library each cell is capable of expressing two or more genes, or gene products, encoding for the subunits of the immunoreceptor. The invention is further related to a method of screening a library of expressor cells as created according to the above method, for one cell that expresses an immunoreceptor that has specificity for a given target molecule.

Abstract: In example implementations, an apparatus is provided. The apparatus includes a dielectrophoresis (DEP) separator, an electrical field generator, a tracking system, and a controller. The DEP separator is to separate a plurality of different particles. The electrical field generator is coupled to the DEP separator to apply a frequency to the DEP separator. The tracking system is to track a movement of a type of particles in the DEP separator. The controller is in communication with the electrical field generator to control the frequency and the tracking system to track the separation. The controller is to calculate a cross-over frequency from a cross-over frequency distribution for the type of particles based on a frequency sweep performed on the type of particles and the movement of the type of particles that is tracked.

Abstract: Various aspects described herein relate to electrochemical devices, e.g., for separation of one or more target organic or inorganic molecules (e.g., charged or neutral molecules) from solution, and methods of using the same. In particular embodiments, the electrochemical devices and methods described herein involve at least one redox-functionalized electrode, wherein the electrode comprises an immobilized redox-species that is selective toward a target molecule (e.g., charged molecule such as ion or netural molecule). The selectivity is based on a Faradaic/redox-activated chemical interaction (e.g., directional hydrogen binding) between the oxidized state of the redox species and a moiety of the target molecule (e.g., charged molecule such as ion or netural molecule).

Abstract: Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.

Abstract: Provided is an implantable biosensor including an intermediate layer; a first electrode layer provided on one surface of the intermediate layer and including a first electrode configured to react with a bio material and an auxiliary electrode electrically connected to the first electrode; and a second electrode layer provided on another surface of the intermediate layer to face the first electrode layer and including a second electrode operating as a reference electrode.

Abstract: It is an object of the present invention to provide a method or an immunochromatographic test piece, which controls a speed or a direction of development of a specimen on the immunochromatographic test piece, so that a treatment with an acid reagent, nitrite, and a neutralizing reagent is properly controlled. The present invention provides an immunochromatographic test piece for extracting and measuring a sugar chain antigen in a specimen, the immunochromatographic test piece having a neutralizing region impregnated with a neutralizing reagent upstream of a label region, and further having a solid acid reagent or nitrite region impregnated with a solid acid reagent, or with nitrite, upstream of the neutralizing region, wherein a resin-made sheet is sandwiched between the solid acid reagent or nitrite region the neutralizing region so as to suppress the movement of a reagent or the movement of a specimen solution between the regions.

Abstract: The embodiments disclose an apparatus including a test cartridge configured for inserting into a reader, a sample insertion component coupled to the test cartridge a test sample, a heater device coupled to the test cartridge configured to heat to a predetermined temperature the test sample, a sensor array coupled to the test cartridge consisting of at least one electrochemical sensor for sensing analytes in a sample, a reader configured to gather test related data from the sensor array coupled to the test cartridge, an analyzer coupled to the reader configured for determining test results of each sensor in the sensor array and comparing all the test results for confirmation of a valid test and coherent results, a MCU (Microcontroller Unit) to perform main control and processing functions, and orchestrates all the functionality for the Reader, and a BLE Radio RF link between the MCU and an external processing system.

Abstract: Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.

Abstract: Disclosure relates to a device and a method of using the device for conducting biological and chemical assays on a sample. The device includes two plates. The first plate includes at least a first and a second sample contact areas on its inner surface. Each sample contact area includes spacers having a bottom end fixed on the inner surface and a top end that is distal to the inner surface. The spacers in the first sample contact area differ in height from those in the second sample contact area. The first and second sample contact areas are disposed at different depths along the inner surface of the first plate, so that the top ends of the spacers are aligned on the same flat plane. The method includes depositing the sample onto the sample contact areas, compressing the sample into a thin layer, and analyzing the sample.

Abstract: An analytical toilet comprising a bowl adapted to receive excreta; a channel in fluid communication with the bowl for extracting a sample of excreta from the bowl; an imaging section in the channel, comprising a transparent window; and a movable wall capable of moving between a first and a second position; wherein the channel is open when the movable wall is in a first position; and wherein the channel is nearly closed when the movable wall is in a second position; a first actuator adapted to move the movable wall between its first and second position; an imaging sensor positioned to image the contents of the channel at the point of the movable wall through the transparent window is disclosed.

Abstract: The invention provides devices and methods for moving charged molecules into and out of tissue samples. This invention is particularly useful for removing endogenous heterogenous particles from tissue samples and for introducing exogenous charged molecules (e.g., antibodies, dyes) into tissue samples.

Abstract: A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.

Abstract: Described herein are reagent compositions for detecting and/or measuring analytes in a test sample. In one embodiment, reagent compositions are described for detecting and/or measuring glucose in a sample.

Abstract: A method for collecting a fine particle stored in a structure by suctioning the fine particle using a nozzle, in which, as the structure, a structure in which at least one communication portion that communicates a space storing the fine particle with one surface side and the other surface side of the structure is formed is used.

Abstract: A biosensor may provide a magnetoresistive (MR) film comprising a nonmagnetic layer may be sandwiched between the two ferromagnetic layers. The MR film may be positioned on a substrate, where the edges of the MR film are in contact with leads. Additionally, the leads may be in contact with pads. The sensors may provide quasi-digital readout that enable greatly enhanced sensitivity. In some embodiments, biosensors may be arranged as array of sensors. The array of sensors may be arranged as a symmetric or asymmetric N1×N2 array, where N1 and N2 are integers, N1 represents the number of sensors linked together in series, and N2 represents the number of sensor sets in parallel, where each sensor set may comprise one or more sensors. Further, the array of sensors may be coupled to a voltmeter, which may be a single voltmeter in some cases that allows the sensors to all be probed simultaneously.

Abstract: The embodiments disclose a method including making electrodes using an electrically conductive material for impedimetric detection of analytical targets in an electrochemical sensing platform device, collecting patient samples in a solution compartment of the electrochemical sensing platform device, binding primers and aptamers to electrodes for functionalizing electrodes for detecting and binding targeted viruses and bacterial pathogens, incubating oral fluid samples in the solution compartment using a heater, measuring electrode impedance changes, recording measured electrode impedance changes in a memory device, and integrating wireless technologies into the electrochemical sensing platform device configured for transmitting recorded measured data.

Abstract: The invention relates to an immunochromatographic device which contains a nitrous acid compound and an organic acid or an organic acid derivative and which is for detecting a detection target in an analyte wherein a sample droplet-receiving member, a labeling substance-holding member, a chromatography medium member and an absorption member are arranged in a manner that a sample develops in this order and wherein a part containing the nitrous acid compound and a part containing the organic acid or the like are at upstream positions from the labeling substance-containing part, and the part containing the nitrous acid compound and the part containing the organic acid or the like are not substantially in contact with each other in the thickness direction.

Abstract: Various fluidic structures are described that may be implemented between two portions of material, at least one of which may be made of a flexible but inelastic material, such as Mylar. Such fluidic circuits may be configured such that applying a clamping pressure zone to such fluidic circuits and then moving the clamping pressure zone along the fluidic circuit and along a particular axis may cause the fluidic circuit to operate in a particular manner, thereby driving fluids contained within the fluidic circuit around the fluidic circuit according to a desired flow sequence.

Abstract: An apparatus for in-vivo measuring H2O2 oxidation within a living tissue. The apparatus includes an electrochemical probe and an electrochemical stimulator-analyzer. The electrochemical probe includes a sensing part and a handle. The sensing part includes a working electrode, a counter electrode, and a reference electrode. The working electrode includes a first biocompatible conductive needle coated with a layer of vertically aligned multi-walled carbon nanotubes. The counter electrode includes a second biocompatible conductive needle. The reference electrode includes a third biocompatible conductive needle. The electrochemical stimulator-analyzer is configured to generate a set of electrical currents in a portion of the living tissue.

Abstract: Systems for detecting fluorescence from a molecule comprising an ion-impermeable film comprising at least one ion-conducting nanopore; a first and second liquid reservoir separated by the film; a means to induce movement of the molecule from the first reservoir to the second reservoir via the nanopore; a light source capable of exciting the molecule to emit fluorescence, wherein the light source shines into the second reservoir; a metallic layer adhered to the film by an adhesion layer and comprising a nanowell structure located adjacent to the nanopore; and a detector configured to detect the fluorescence emitted by the molecule are provided. Methods of use of the systems are also provided.

Abstract: The present invention relates to a device (10; 30; 50) for analysing liquid samples comprising amplified nucleic acids, said device comprising: a sample pad (16); a first (18) and a second (19) sample analysis strip configured to analyse different aspects of the nucleic acid sample wherein said segments comprise nucleic acid sequences which are complementary to the predetermined sequences of target nucleic acids whose presence or absence is analysed; and a housing (11) enclosing said sample pad and at least two elongated sample analysis strips wherein the analysis result is detectable from the outside of the housing from the combination of aligned segments of said first and second analysis strips. The invention furthermore relates to a method for determining the presence or absence of a target nucleic acid, providing confirmatory results, in combination with a predetermined sequence in a sample from a subject using the device according to the invention.

Abstract: An electrochemical biosensor includes a working electrode modified with a redox polymer and amine-terminated capture aptamer specific for a particular detection target. The binding sequence of the capture aptamer is also complementary to part of a second ssDNA which is labeled with HRP (horseradish peroxidase). The capture aptamer will form dsDNA with the HRP-labeled ssDNA and bring HRP into electrical contact with the redox polymer and the electrode. Prior to capturing the detection target, addition of H2O2 will lead to the highest reduction current due to the redox polymer-mediated, enzyme-amplified electroreduction of H2O2.

Abstract: The invention is a slide cleaner apparatus. The apparatus includes a cleaning chamber configured to receive a cell counting slide, at least one fluid inlet arranged to align with an inlet on a cell counting slide when located in the chamber, and configured to feed cleaning fluid into the cleaning chamber and slide, and at least one fluid outlet configured to remove cleaning fluid from the cleaning chamber and slide. The invention also includes methods of cleaning a cell counting slide.

Abstract: Mesofluidic devices for culturing cell aggregates and methods of using the same are disclosed. An exemplary mesofluidic device comprises at least one fluid inlet, at least one fluid outlet, a plurality of fluid channels, and a plurality of culture chambers. Each culture chamber can comprise at least one chamber inlet and at least one chamber outlet. The at least one chamber inlet can be in fluid communication with the at least one fluid inlet via at least one of the plurality of fluid channels. The at least one chamber outlet can be in fluid communication with the at least one fluid outlet via at least one of the plurality of fluid channels. The mesofluidic device can be configured to contain a cell aggregate in each of the plurality of culture chambers.

Abstract: A laboratory system and a method for separating interfering substances contained in test samples is presented. The laboratory system comprises separation vessels comprising solid surfaces and capturing molecules which are immobilized on the solid surfaces. The capturing molecules of the separation vessels are configured to bind interfering substances of laboratory tests of different analytical methods.

Abstract: The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc.

Abstract: Presented herein are systems, methods for collecting fluid from a surface (e.g., skin) and analyzing the fluid (e.g., to measure chemical, physical and/or biological properties of the fluid). For example, a system for fluid collection on a surface (e.g., skin) and fluid analysis includes at least one of the following modules: (i) a collection and delivery module to collect a fluid over a wet or partially wet surface and deliver it to (ii) a main sensing module to perform chemical, physical and/or biological analysis on the fluid. The system also includes (iii) a flow regulation module, for controlling fluid flow (e.g., transport) through the system and (iv) a waste module to collect and/or dispose of the fluid after analysis is complete.

Abstract: A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem including an actuation substrate, and a set of pins interacting with the actuation substrate, and a spring plate configured to bias at least one pin in a configurations, the valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; and a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols.

Abstract: A spectrometry device includes a light source, an integrator configured to have an internal space in which a long afterglow emission material is disposed and output detection light from the internal space, a spectroscopic detector, an analysis unit configured to analyze a photoluminescence quantum yield of the long afterglow emission material, and a control unit configured to control switching between presence and absence of input of excitation light to the internal space and an exposure time in the spectroscopic detector. The control unit controls the light source so that the input of the excitation light to the internal space is maintained in a first period and the input of the excitation light to the internal space is stopped in a second period, and controls the spectroscopic detector so that an exposure time in the second period becomes longer than an exposure time in the first period.

Abstract: A test device is provided that can comprise: a housing accommodating a chromatography support, wherein the housing comprises: a supporting part that supports a container accommodating a liquid used for chromatography. A method is provided for performing chromatography using the test device.

Abstract: An object of the present invention is to provide a novel method for designing a primer ensuring reactivity and discriminatory power in a method for detecting a single base substitution based on an ASP-PCR method and to provide a method for easily detecting multiple point mutations within overlapping amplicons, particularly, two adjacent single base substitutions. The single base substitutions can easily be detected by using a mutant primer in which the base of the third nucleotide from the 3? end corresponds to the base of a mutant nucleotide of a single base substitution contained in a nucleic acid sample, in which the base of the second nucleotide from the 3? end is not complementary to the base of the corresponding nucleotide of the nucleic acid, and in which the bases of the other nucleotides are complementary to the bases of the corresponding nucleotides of the nucleic acid.

Abstract: The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Tumor Suppressor genes, in particular, by targeting natural antisense polynucleotides of Tumor Suppressor genes. The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of Tumor Suppressor genes.

Abstract: A dispensing device which has a dispensing channel extending between a supply opening and an output opening into the course of which a pump, a selection valve and a dispensing valve are connected. The selection valve is also connected to a pressurised air source. The dispensing device allows a dispensed output of a fluid, wherein a buffer channel portion is filled by the pump in a pump dispensing phase, to which buffer channel portion pressure is applied from the pressurised air source during a pressurised air dispensing phase such that a precise fluid output is subsequently possible by clocked actuation of the dispensing valve until the target fluid amount has been reached.

Abstract: An optofluidic diagnostic system and methods for rapid analyte detections. The system comprises an optofluidic sensor array, a test plate and an optical detection cartridge. The sensor array supports one or more distinct sensor units, each having a reactor section designed to temporarily enter a series of different kinds of wells in the test plate. One kind of well is a sample reservoir that holds reagent solution to be transferred into the reactor section. Another kind of well is a drainage chamber that removes reagent solution from the reactor section. A third kind of well is a colorant reservoir that holds a colorant reagent transferable into a reactor section. Finally, the sensor unit is transferred to the optical detection cartridge where it is placed into an isolation booth during the optical detection process so that its flat observation face is stationed in a viewing window opposite an optical detector lens.

Abstract: Provided herein are systems and methods for performing assays. In particular, provided herein are systems and methods for performing sensitive and rapid immunoassays.

Abstract: Among other things, the present invention is related to devices and methods of performing biological and chemical assays, particularly an easy sample manipulation and/or a rapid change or a rapid thermal cycling of a sample temperature is needed (e.g. Polymerase Chain Reaction (PCR) for amplifying nucleic acids).

Abstract: This invention relates to sequence specific electrochemically-labeled oligonucleotide probes for the detection of nucleic acids and methods associated therewith.

Abstract: According to one embodiment, a molecule detecting device includes a capturing section, a releasing section, and a detecting section. The capturing section is configured to, by combining a target molecule and a solubilizing agent with each other and thereby creating a composite body, capture the target molecule in a carrier liquid. The releasing section is configured to make the composite body release the target molecule therefrom in the carrier liquid. The detecting section is configured to carry out detection of the target molecule in the carrier liquid.