Measuring Or Testing For Antibody Or Nucleic Acid, Or Measuring Or Testing Using Antibody Or Nucleic Acid Patents (Class 435/287.2)
  • Patent number: 11119110
    Abstract: Compositions and methods for measuring coagulation parameters using very small volumes of blood are provided. Advantageously, the methods described herein can be performed from a single drop of blood (about 20 ?L) while generally leaving enough sample to perform other measurements, optionally in a multiplexed format. The methods and devices do not require a skilled operator and can be performed at the point of service, which can be an important feature for managing blood coagulation disorders and treatments thereof.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: September 14, 2021
    Assignee: Labrador Diagnotics LLC
    Inventors: Mark Dayel, Samartha Anekal, Paul Patel, Ian Gibbons, Elizabeth A. Holmes
  • Patent number: 11112404
    Abstract: Disclosed is a method for obtaining information of a test substance, the method including: forming a complex by causing a capture substance to bind to a test substance in a specimen; selectively collecting at least the complex from the specimen; immobilizing the complex collected from the specimen, onto a base plate; and obtaining information regarding a structure of the test substance from the complex immobilized on the base plate.
    Type: Grant
    Filed: April 12, 2018
    Date of Patent: September 7, 2021
    Inventors: Kenji Akama, Toshihiro Watanabe, Masaya Okada, Kazuto Yamashita, Akshay Ganguly, Shigeki Iwanaga
  • Patent number: 11090651
    Abstract: The present technology provides spontaneous capillary flow devices for patterning walls on a hydrophilic substrate. In some embodiments, the devices include rails having a first end portion for receiving a flowable material, a second end portion opposite the first end portion, and a base portion having a flow surface extending between the first end portion and the second end portion. The flow surface can face the hydrophilic substrate and define a flow path. When the flowable material is released into the first end portion, the flowable material flows via spontaneous capillary flow from the first end portion to the second end portion along the flow path to create a partition on the hydrophilic substrate.
    Type: Grant
    Filed: May 31, 2019
    Date of Patent: August 17, 2021
    Assignee: University of Washington
    Inventors: Ashleigh Theberge, Erwin Berthier, Ilham Wilson, John Day, Tianzi Zhang, Ulri Lee
  • Patent number: 11087467
    Abstract: Systems and methods for testing visible chemical reactions of a reagent are provided. In one implementation, the method may include receiving from an image sensor associated with a mobile communications device an image of a reagent with a plurality of colored test reagent pads in proximity to a colorized surface having a plurality of colored reference elements of differing shades. The method further includes using the differing shades of the plurality of colored reference elements to determine local illumination conditions. Thereafter, the method includes using the determined local illumination conditions and an analysis of a depiction of the plurality of colored test reagent pads in the image to determine an extent of a chemical reaction on the reagent. Then the method includes causing the mobile communications device to provide to the user an indication that the testing of the reagent is complete.
    Type: Grant
    Filed: December 23, 2019
    Date of Patent: August 10, 2021
    Assignee: HEALTHY.IO LTD.
    Inventors: Yonatan Adiri, Ido Omer, Shachar Mendelowitz, Roee Salomon
  • Patent number: 11078527
    Abstract: According to a first aspect of the present invention, a method is provided for detecting and/or quantifying male genomic DNA in a sample, wherein the method comprises the step of amplification of a multicopy locus within the human Y-chromosome (MCL-Y), wherein said locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 3 over a stretch of at least 60 base pairs (bp). A second aspect of the present invention relates to a primer or primer pair which hybridizes under stringent conditions to a sequence according to SEQ ID NO. 3 and/or any of 4 to 11. The invention also relates to a kit.
    Type: Grant
    Filed: September 15, 2017
    Date of Patent: August 3, 2021
    Assignee: Qiagen GmbH
    Inventors: Miroslav Vranes, Ralf Peist, Mario Scherer, Stefan Otto Cornelius, Margaretha König
  • Patent number: 11073761
    Abstract: A semiconductor resist composition includes an organometallic compound represented by Formula I and a solvent: wherein L includes at least one alkylene group in the main chain. A pattern may be formed using the composition. The pattern formed by using the semiconductor resist composition may not collapse while having a high aspect ratio.
    Type: Grant
    Filed: December 5, 2018
    Date of Patent: July 27, 2021
    Assignee: Samsung SDI Co., Ltd.
    Inventors: Kyung Soo Moon, Jaehyun Kim, Yoong Hee Na, Ran Namgung, Hwansung Cheon, Seungyong Chae
  • Patent number: 11059039
    Abstract: A “smart” instrument for mixing (100), a microfluidic chip (50), and a system wherein they are used to prepare formulations is provided. The microfluidic chip comprises microchannels and a programmable data component. The system achieves optimal formulations for RNA, antisense, peptides and small molecules in the hands of even novice users.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: July 13, 2021
    Assignee: Precision NanoSystems Inc.
    Inventors: Andre Wild, Timothy Leaver, Robert James Taylor, Euan Ramsay, Nicolas Klaassen, Shao Fang Shannon Chang, Keara Marshall
  • Patent number: 11027272
    Abstract: Disclosed are transfer dispensers for assay devices. These dispensers provide for transfer of a single assay component into a single well in the assay device. This ensures that the assay conducted in each well contains only a single component.
    Type: Grant
    Filed: January 28, 2020
    Date of Patent: June 8, 2021
    Assignee: Plexium, Inc.
    Inventors: Kandaswamy Vijayan, Kapil Mahakalkar, Yi Zhang
  • Patent number: 11027281
    Abstract: A cartridge reader controlled by processing means for carrying out a diagnostic test on a sample contained in a fluidic cartridge comprises a mechanical valve for isolating the sample with the cartridge. A system for actuating the mechanical valve comprises an actuation member configured to move the mechanical valve from an open position to a closed position and an armature connected to the actuation member. The armature is configured to engage an electromagnet, wherein the electromagnet can be switched between an active state in which it electromagnetically holds the armature and an inactive state in which it does not electromagnetically hold the armature. First biasing means are disposed between the actuation member and a bearing surface, wherein the first biasing means is configured to bias the actuation member into a first position in which it actuates a mechanical valve in a fluidic cartridge inserted into the reader.
    Type: Grant
    Filed: February 2, 2016
    Date of Patent: June 8, 2021
    Assignee: Binx Health Limited
    Inventors: Ben Arlett, Tom Edwards
  • Patent number: 11020741
    Abstract: A system and method for performing a portable, fast, field assay of a small sample biological analyte includes a microfluidic cartridge and a reader with which the microfluidic cartridge is selectively communicated. A closed microfluidic circuit mixes and recirculates the analyte with a buffer. A shear horizontal surface acoustic wave (SAW) detector communicates with the microfluidic circuit and has a plurality of channels including at least one functionalized sensing channel in which the mixed analyte and buffer is recirculated and sensed. Capture of the analyte is amplified by recirculation of the analyte and buffer, and detection is amplified by use of an all-purpose endospore display mass amplification.
    Type: Grant
    Filed: December 10, 2018
    Date of Patent: June 1, 2021
    Assignee: Autonomous Medical Devices Inc.
    Inventors: Yehoshua Shachar, Roger Kornberg
  • Patent number: 11006617
    Abstract: A system for determining the gender and/or fertility status of avian eggs including a sampling apparatus and an electromagnetic radiation transmitter and detector. In certain embodiments, the transmitter operates in the terahertz range. The sampling apparatus can be coupled to an avian egg. The sampling apparatus includes a vacuum source, a gas collection device, and a membrane that can be positioned in the passageway coupling the vacuum source to the gas collection device. The membrane is capable of capturing volatile organic compounds. The sampling apparatus applies a vacuum from the vacuum source to the gas proximate to the avian egg and directs the gas captured from the vicinity of the egg toward the membrane. Subsequently, the membrane is positioned within the electromagnetic radiation emitted by the transmitter, generating a spectrum which can be analyzed to determine whether the egg is fertile or infertile, and if fertile, whether the egg is male or female.
    Type: Grant
    Filed: July 31, 2017
    Date of Patent: May 18, 2021
    Assignee: NOVATRANS GROUP S.A.
    Inventors: Paul Knepper, Matthew O'Hayer, Jonathan Hoopes, Eran Gabbai
  • Patent number: 11009475
    Abstract: The present invention generally relates to doped, metal oxide-based sensors, wherein the doped-metal oxide is a monolayer, and platforms and integrated chemical sensors incorporating the same, methods of making the same, and methods of using the same.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: May 18, 2021
    Assignee: VAON, LLC
    Inventors: Vladimir Dobrokhotov, Alexander Larin
  • Patent number: 10962531
    Abstract: A first sample solution including exosomes including first to third detection target substances is mixed with a first buffer solution including first nanoparticles including first binding substances which bind to the first detection target substances. The first detection target substances and the first binding substances are bound together, so as to form first complexes of the exosomes and the first nanoparticles. The first complexes are isolated from a mixed solution of the first sample solution and the first buffer solution. The second detection target substances and the second binding substances are bound together, so as to capture the first complexes on a substrate. The second binding substances are fixed onto the substrate. A second buffer solution including second nanoparticles including third binding substances which bind to the third detection target substances is reacted with the first complexes.
    Type: Grant
    Filed: February 15, 2018
    Date of Patent: March 30, 2021
    Inventors: Makoto Itonaga, Yuichi Hasegawa, Koji Tsujita, Masayuki Ono, Shigehiko Iwama, Makoto Igarashi
  • Patent number: 10953403
    Abstract: An analysis system and a method for testing, in particular, a biological sample, the sample and/or analytes of the sample being temperature-controlled in advance or denatured immediately before the test, and/or being heated, the heat being transferred through a chip of a sensor apparatus.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: March 23, 2021
    Inventors: Guenter Bruckmann, Erol Meyda, Axel Niemeyer, Harald Pauls
  • Patent number: 10913067
    Abstract: An electrowetting on dielectric (EWOD) device includes an EWOD device array that applies electrowetting forces and contains a non-polar fluid. A barrier droplet configuration is formed using electrowetting forces to obstruct migration of a species from a first area of the EWOD device array to a protected area of the EWOD device array. A method of operating the EWOD device includes the steps of: dispensing a source droplet into a first area of the EWOD device array, the source droplet containing a migrating species, wherein the EWOD device array includes a second area to be protected from the migrating species; and forming a barrier droplet configuration positioned between the first area and the second area of the EWOD device array that obstructs a migration pathway of the migrating species between the first area and the second area.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: February 9, 2021
    Assignee: Sharp Life Science (EU) Limited
    Inventors: Adam Christopher Wilson, Sally Anderson, Peter Neil Taylor, Campbell Donald Brown, Pamela Ann Dothie, Laura Huang
  • Patent number: 10900981
    Abstract: Embodiments of the present disclosure generally pertain to systems and methods for performing amplicon rescue multiplex polymerase chain reaction (arm-PCR). In one embodiment, the system comprises a processor and a reader coupled to a control element. The control element is configured to control the operation of the processor and the reader based on a variety of settings. The processor is configured to receive a self-contained cassette for performing PCR amplification of DNA and/or RNA obtained from an organic specimen. The processor engages with the cassette and manipulates reagents within the cassette in order to amplify and detect the DNA from the specimen. The processor also causes the cassette to deposit the DNA on a microarray within the cassette. The reader is configured to receive the cassette after it has been processed by the processor and to capture an image of the microarray for transmission to the control element as test data.
    Type: Grant
    Filed: December 5, 2018
    Date of Patent: January 26, 2021
    Assignee: iCubate, Inc.
    Inventors: Jeff Bertrand, Jian Han, Phillip Ewing
  • Patent number: 10890559
    Abstract: An ITP-based system and a method are provided. ITP is used to focus a sample of interest and deliver a high concentration target to a pre-functionalized surface comprising immobilized probes, thus enabling rapid reaction at the sensor site.
    Type: Grant
    Filed: November 28, 2014
    Date of Patent: January 12, 2021
    Inventors: Moran Bercovici, Merav Karsenty
  • Patent number: 10875016
    Abstract: A micro analysis chip comprises an inlet and a fluid flow path communicating thereto. The fluid flow path comprises a first flow path, a second flow path, and a third flow path arranged continuously along a longitudinal direction of the fluid flow path. An antibody is bound on at least one peripheral surface selected from the group consisting of peripheral surfaces of the second and third flow paths. A cross-sectional area of the third flow path is constant or increased monotonically along a direction X from the second flow path toward the third flow path. A cross-sectional area of the second flow path is increased monotonically along the direction X from the one end to the other end of the second flow path. A cross-sectional area of the first flow path is larger than a cross-sectional area at the one end of the second flow path.
    Type: Grant
    Filed: February 20, 2018
    Date of Patent: December 29, 2020
    Inventors: Shuji Sato, Yasuaki Okumura, Yukari Nishiyama, Tatsurou Kawamura, Ben Jones, Liesbet Lagae, Tim Stakenborg
  • Patent number: 10861829
    Abstract: A system includes an image sensor structure and a flow cell. The image sensor structure includes an image layer disposed over a base substrate. A device stack is disposed over the image layer. A bond pad is disposed in the device stack. A passivation stack is disposed over the device stack and the bond pad. An array of nanowells is disposed in a top layer of the passivation stack. A through-silicon via (TSV) is in electrical contact with the bond pad. The TSV extends through the base substrate. A redistribution layer (RDL) is disposed on a bottom surface of the base substrate. The RDL is in electrical contact with the TSV. The flow cell is disposed upon the top layer of the passivation stack to form a flow channel therebetween. The flow channel is disposed over the array of nanowells and the bond pad.
    Type: Grant
    Filed: December 26, 2017
    Date of Patent: December 8, 2020
    Assignee: ILLUMINA, INC.
    Inventors: Tracy Helen Fung, Hai Quang Tran
  • Patent number: 10852274
    Abstract: A method of target molecule detection includes simultaneously obtaining a first signal from a first working electrode and a second signal from a second working electrode, wherein the first signal is responsive to interaction of the first recognition element with the target molecule in a sample, and the second signal is indicative of background noise from the sample. The method further includes generating a modified signal that is proportional to an instantaneous difference between the first and second signals, wherein the modified signal indicates an amount of the target molecule present in the sample.
    Type: Grant
    Filed: March 9, 2018
    Date of Patent: December 1, 2020
    Assignee: Auburn University
    Inventors: Christopher J. Easley, Mark D. Holtan, Subramaniam Somasundaram
  • Patent number: 10837061
    Abstract: Methods, systems, and devices are disclosed for enrichment and detection of molecules of a target biomarker. In one aspect, In one aspect, a biosensor device for enriching and detecting biomarker molecules include a substrate, and a microarray of hydrophilic islands disposed on the substrate. A sensing area on each of the microarray hydrophilic islands is structured to anchor bio-molecular probes of at least one type for detecting molecules of a target biomarker and to attract an array of nanodroplets of a biomarker solution that includes the target biomarker molecules. A hydrophobic surface is disposed to surround the microarray of hydrophilic islands.
    Type: Grant
    Filed: August 23, 2018
    Date of Patent: November 17, 2020
    Assignees: The Regents of the University of California, Compliance Decisions, Inc.
    Inventors: Yu-Hwa Lo, Wen Qiao, Tiantian Zhang, Ian Lian, Tony Minghung Yen
  • Patent number: 10823660
    Abstract: Techniques are disclosed relating to fluorescence-based flow cytometry. A flow cytometer may include a partially-reflective surface configured to reflect a first portion of fluorescent emissions from a sample to a first optical sensor and direct a second, greater portion of fluorescent emissions from the sample to a second optical sensor and a controller configured to determine a value representing the intensity of the fluorescent emissions based on a first measurement taken by the first optical sensor, a second measurement taken by the second optical sensor, or both. A flow cytometer may include a baseplate with a first side and a second, opposing side with a flow cell, a laser, and a reflective surface disposed above the first side and an optical sensor and isolating material disposed below the second side. The reflective surface receives fluorescent emissions and reflects at least a portion through the baseplate to the optical sensor.
    Type: Grant
    Filed: April 20, 2020
    Date of Patent: November 3, 2020
    Assignee: Chandlertec Inc.
    Inventor: Van S. Chandler
  • Patent number: 10814326
    Abstract: A nucleic acid analyzer includes a first container setting part that sets a first container including reagent storages which store reagents for nucleic acid extraction such that the reagent storages are provided along an X-axis extending in a longitudinal direction of the first container; a dispensing unit that transfers, along the-X axis from the first container, extraction liquid containing nucleic acids extracted in the first container using the reagents for nucleic acid extraction; a second container setting part that is provided along the X-axis and sets a second container, the second container including an injection port through which the extraction liquid transferred by the dispensing unit is injected, storages that store reagents for amplifying the nucleic acids in the extraction liquid, and a flow path that connects the injection port and the storages; and a detector that detects a nucleic acid amplification reaction, which occurs in the storages.
    Type: Grant
    Filed: October 19, 2018
    Date of Patent: October 27, 2020
    Inventors: Toshiyuki Ozawa, Masanori Imazu, Hironori Kobayashi, Nobuhiro Kitagawa, Yoshihiko Kitawaki
  • Patent number: 10814321
    Abstract: At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.
    Type: Grant
    Filed: June 4, 2018
    Date of Patent: October 27, 2020
    Assignee: Canon U.S.A., Inc.
    Inventors: Hiroshi Inoue, Ivor T. Knight, Gregory A. Dale, Rita R. Colwell
  • Patent number: 10802292
    Abstract: A synthetic aperture optics (SAO) imaging method minimizes the number of selective excitation patterns used to illuminate the imaging target, based on the objects' physical characteristics corresponding to spatial frequency content from the illuminated target and/or one or more parameters of the optical imaging system used for SAO. With the minimized number of selective excitation patterns, the time required to perform SAO is reduced dramatically, thereby allowing SAO to be used with DNA sequencing applications that require massive parallelization for cost reduction and high throughput. In addition, an SAO apparatus optimized to perform the SAO method is provided. The SAO apparatus includes a plurality of interference pattern generation modules that can be arranged in a half-ring shape.
    Type: Grant
    Filed: September 11, 2019
    Date of Patent: October 13, 2020
    Assignee: Optical Biosystems, Inc.
    Inventors: Chun-Sheu Lee, Jong Buhm Park
  • Patent number: 10788481
    Abstract: The invention relates to methods to evaluate in one single assay the biocompatibility of materials based on the simultaneous determination of the phagocytosis, cell activation and cell death produced by said materials, preferably, in peripheral blood or other human cells and proximal fluids. The invention also relates to a kit to perform the method of the invention.
    Type: Grant
    Filed: July 5, 2017
    Date of Patent: September 29, 2020
    Inventors: Manuel Fuentes Garcia, Paula Diez Garcia, Cristina Isabel Gonçalves Grunho Tedosio, Alberto Orfao de Matos, Ricardo Jara Acevedo
  • Patent number: 10786812
    Abstract: Provided are a medical analysis device and a cell analysis method which can capture many cancer cells, including cancer cells not expressing EpCAM. The medical analysis device includes a flow channel zone, and also includes a chamber zone. The inner surface of the flow channel zone is at least partially provided with a layer of a hydrophilic polymer having a thickness of 2 to 200 nm.
    Type: Grant
    Filed: April 24, 2017
    Date of Patent: September 29, 2020
    Inventors: Yasuhisa Minagawa, Masaru Tanaka, Takashi Hoshiba, Tomokazu Shibuya
  • Patent number: 10780371
    Abstract: Emulsion breaking and phase separation is achieved by droplet adhesion. An emulsion breaking device includes a channel having distinct adjacent zones with distinctly different surface wettability characteristics, namely, solvophilic and solvophobic surfaces. The device is positioned such that the upstream portion of the device is configured to be wetted by the continuous phase of the emulsion, and the downstream portion of the device is configured to be wetted by the dispersed phase of the emulsion. As the emulsion flows from the upstream zone to the downstream zone, the change in surface wettability characteristics promotes adhesion of the dispersed phase as the dispersed phase wets the surface of the downstream portion of the channel, which results in breaking of the emulsion. Subsequent collection of the broken emulsion in a collection vessel results in separation of the disparate phases to facilitate their recapture and recycling.
    Type: Grant
    Filed: November 2, 2018
    Date of Patent: September 22, 2020
    Inventors: Howard A. Stone, Jiang Li, Haosheng Chen
  • Patent number: 10781491
    Abstract: Biomarkers, methods, assays, and kits are provided for predicting the progression of Barrett esophagus in a subject to esophageal cancer. The method can therefore also be used to select the appropriate treatment for a subject diagnosed with Barrett esophagus. Disclosed are methods for predicting the progression of Barrett esophagus in a subject to esophageal cancer. The method can therefore also be used to select the appropriate treatment for a subject diagnosed with Barrett esophagus. For example, the disclosed biomarkers, methods, assays, and kits can be used to predict the benefit of surgery, laser treatment, radiofrequency ablation, radiation therapy, chemotherapy, or any combination thereof for a subject diagnosed with Barrett esophagus based on whether they are predicted to progress to esophageal cancer.
    Type: Grant
    Filed: March 16, 2015
    Date of Patent: September 22, 2020
    Assignee: H. Lee Moffitt Cancer Center and Research Institute, Inc.
    Inventors: Domenico Coppola, Xiaotao Qu
  • Patent number: 10774304
    Abstract: A portable and mobile bioprocessing system and method for protein manufacturing that is compact, integrated and suited for on-demand production of any type of proteins and for delivery of the produced proteins to patients or for assay purposes. The portable system and method can also be used for efficient on-demand production of any type of protein with point-of-care delivery.
    Type: Grant
    Filed: October 23, 2019
    Date of Patent: September 15, 2020
    Inventors: Govind Rao, Yordan Kostov, Leah Tolosa, Xudong Ge, Douglas Frey, Shayan Gholipour Borhani, David Burgenson
  • Patent number: 10775370
    Abstract: A fluidic testing system and method for use are presented. The fluidic testing system includes a microfluidic channel, a first chamber and second chamber. The microfluidic channel has only one port for the introduction and/or extraction of fluid through the microfluidic channel. The first chamber is disposed at a terminal end of the microfluidic channel. The second chamber is coupled to the fluidic channel and is aligned such that each opening to the second chamber is configured to be aligned substantially parallel to a gravity vector during operation.
    Type: Grant
    Filed: July 14, 2016
    Date of Patent: September 15, 2020
    Inventors: Jordi Carrera Fabra, Mathias Kuphal, Rafael Bru Gilbert, Anna Comengés Casas
  • Patent number: 10752698
    Abstract: The present disclosure relates to a method for identifying cells that express antigen-specific antibodies with a high binding affinity for a monomeric antigen. Using fluorescence activated cell sorting, cells expressing high affinity antigen-specific antibodies are selected from a population of immune cells isolated from a mammal that has been immunized with or otherwise exposed to the antigen. Nucleic acids encoding the high affinity antibodies can then be cloned into other lymphoid and non-lymphoid cells where the antibody can be expressed and from which the antibodies can be secreted.
    Type: Grant
    Filed: November 13, 2015
    Date of Patent: August 25, 2020
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Gang Chen, Robert Babb, James P. Fandl
  • Patent number: 10750950
    Abstract: The invention relates to a device (100) and a corresponding method for thermoacoustic sensing, in particular thermoacoustic imaging, the device (100) comprising: a) an irradiation unit (10) configured to generate electromagnetic and/or particle energy exhibiting a first modulation, the first modulation comprising at least one frequency and to continuously emit the energy towards a target (1), whereby acoustic waves are continuously generated in the target, the acoustic waves exhibiting a second modulation, the second modulation comprising the at least one frequency and/or a harmonic frequency of the at least one frequency; b) a detection unit (20) configured to simultaneously detect the acoustic waves exhibiting the second modulation while the energy exhibiting the first modulation is being continuously emitted towards the target (1); and c) a processing unit (30) configured to determine at least one thermoacoustic value of an amplitude and/or a phase of the second modulation of the acoustic waves at the at l
    Type: Grant
    Filed: April 23, 2015
    Date of Patent: August 25, 2020
    Assignee: Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)
    Inventors: Stephan Kellnberger, Pouyan Mohajerani, Vasilis Ntziachristos
  • Patent number: 10752496
    Abstract: Methods are provided for manufacturing well-controlled, solid-state nanopores and arrays thereof. In one aspect, methods for manufacturing nanopores and arrays thereof exploit a physical seam. One or more etch pits are formed in a topside of a substrate and one or more trenches, which align with the one or more etch pits, are formed in a backside of the substrate. An opening is formed between the one or more etch pits and the one or more trenches. A dielectric material is then formed over the substrate to fill the opening. Contacts are then disposed on the topside and the backside of the substrate and a voltage is applied from the topside to the backside, or vice versa, through the dielectric material to form a nanopore. In another aspect, the nanopore is formed at or near the center of the opening at a seam, which is formed in the dielectric material.
    Type: Grant
    Filed: July 30, 2018
    Date of Patent: August 25, 2020
    Inventors: Philip Allan Kraus, Joseph R. Johnson
  • Patent number: 10737263
    Abstract: A method of manufacturing a device useable in DNA or genome sequencing comprises disposing pairs of electrodes on a substrate, the electrodes within each pair separated by a nanogap; depositing a resist layer over the electrodes; patterning the resist layer to create an exposed region on each electrode at or near each nanogap; roughening the electrode surface within each exposed region using various methods; and exposing the exposed regions to biomolecules, wherein one biomolecule bridges each nanogap of each electrode pair, with each end of each biomolecule bound to the electrodes at each exposed region.
    Type: Grant
    Filed: February 9, 2017
    Date of Patent: August 11, 2020
    Assignee: Roswell Biotechnologies, Inc.
    Inventors: Chulmin Choi, Sungho Jin, Paul W. Mola, Barry L. Merriman
  • Patent number: 10722888
    Abstract: Devices having nanochannels suitable for confinement and alignment of DNA molecules, as well as methods of fabricating the same and methods of using the same for DNA analysis, are provided. A device can include a dynamically-controlled, unified microchannel-nanochannel platform suitable for confinement and alignment of DNA molecules. The nanochannels can be reversibly formed within nanoslits formed in a deformable substrate or base layer.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: July 28, 2020
    Assignee: The Hong Kong University of Science and Technology
    Inventors: Shuhuai Yao, Miao Yu, Youmin Hou
  • Patent number: 10710075
    Abstract: Methods and devices for concentrating target cells using dielectrophoresis (DEP) are disclosed. The method allows relatively high throughput of sample through a microfluidic device in order to allow rapid capture of target cells even when they are present in low concentrations within the sample. The method utilizes multiple chambers through which samples will flow, the chambers arranged such that the first capture area has a larger area and faster flow rate than a second chamber, the second chamber being positioned downstream of the first capture area and being smaller with a slower flow rate to further concentrate the material captured in the first capture area.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: July 14, 2020
    Inventors: Jonathan O'Halloran, Heather Murton, Stephen Osborne, Eduardo Boada, Jonathan Salmon
  • Patent number: 10704042
    Abstract: Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.
    Type: Grant
    Filed: April 30, 2018
    Date of Patent: July 7, 2020
    Inventors: John R. Nelson, R. Scott Duthie, Rohini Dhulipala, Gregory A. Grossman, Anuradha Sekher
  • Patent number: 10694984
    Abstract: There is provided a test method for analysing a body fluid in which a test tape is used in a test device to successively provide analytical test fields stored on the test tape, wherein body fluid is applied by a user to the test field provided at a time and the said test field is photometrically scanned using a measuring unit of the device to record measurement signals. To increase the measurement reliability, it is proposed that a control value is determined from a time-dependent and/or wavelength-dependent change in the measurement signals and that the measurement signals are processed as valid or discarded as erroneous depending on the control value.
    Type: Grant
    Filed: February 18, 2019
    Date of Patent: June 30, 2020
    Assignee: Roche Diabetes Care, Inc.
    Inventors: Karl Miltner, Robert Lorenz, Ulrich Porsch, Clemens Knapp
  • Patent number: 10646869
    Abstract: (1) Plural reaction chambers each including at least one cell capturing unit and at least one nucleic acid capturing unit connected via a flow channel, (2) a first liquid flow channel commonly connected to the plural reaction chambers on a first surface side, (3) plural second liquid flow channels connected to the plural reaction chambers in a one-on-one relationship on the first surface side, and (4) a third liquid flow channel commonly connected to the plural reaction chambers on a second surface side opposite to the first surface, are provided in a flow cell device for single cell analysis.
    Type: Grant
    Filed: February 3, 2015
    Date of Patent: May 12, 2020
    Assignee: Hitachi, Ltd.
    Inventors: Masataka Shirai, Tomoyuki Sakai
  • Patent number: 10639633
    Abstract: A controller is connected to controlled devices on a microfluidic chip with a multiplexer. Signal transmission band width is differently allocated amongst the plurality of controlled devices on the microfluidic chip.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: May 5, 2020
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Sirena C. Lu, Manish Giri, Matthew David Smith, Melinda M. Valencia
  • Patent number: 10626432
    Abstract: A composition is provided. The composition comprises an enzyme and a ruthenium- or osmium-based electron transfer agent, wherein the ruthenium- or osmium-based electron transfer agent is a complex [M(A)x(B)y](X)n wherein M is ruthenium or osmium; A is an amine ligand; each B is a ligand different to A; x is an integer selected from 1 to 5; y is an integer selected from 1 to 5; x+y is 6 or 8; n is an integer selected from 1 to 6; and X is any suitable counterion.
    Type: Grant
    Filed: April 28, 2016
    Date of Patent: April 21, 2020
    Inventors: Marco Cardosi, Stephanie Kirkwood
  • Patent number: 10626454
    Abstract: The present disclosure provides compositions and methods for nucleic acid amplification and analysis. In some embodiments, a nucleic acid amplification reaction mixture is formulated to minimize or eliminate the inclusion of a species that can affect activity of one or more inhibitory species in the nucleic acid amplification reaction mixture. Such reaction mixtures can permit suitable analysis of nucleic acid sample prior to their use in one or more downstream assays, including nucleic acid sequencing assays.
    Type: Grant
    Filed: July 26, 2017
    Date of Patent: April 21, 2020
    Assignee: SeqMatic, LLC
    Inventors: Victor Hum, Danny Lee, Kelvin Chan
  • Patent number: 10627336
    Abstract: An optical measurement device with a transparent cylindrical measurement cuvette, which defines an axial symmetrical axis and contains the fluid sample, an optical measurement arrangement which quantitatively determines the optical property of the fluid sample in the measurement cuvette, and a mechanical cleaning arrangement is described. The cleaning arrangement comprises an external magnetic working platform, which is arranged coaxially to the symmetrical axis, is slidable in a translatory manner parallel to the symmetrical axis, is rotatable about the symmetrical axis, encloses the cylindrical measurement cuvette externally in an annular manner, and has at least one translatory magnet element and at least one rotary magnet element, and an internal magnetic cleaning unit, which is arranged within the measurement cuvette and is slidable in a translatory manner, has a cleaning body, has at least one translatory magnet element, and has at least one rotary magnet element.
    Type: Grant
    Filed: November 7, 2017
    Date of Patent: April 21, 2020
    Assignee: HACH LANGE GMBH
    Inventors: Frank Steinhauer, Roman Klein, Robert Dalljo
  • Patent number: 10619202
    Abstract: A device (100) and a method optically control a chemical reaction in a reaction chamber (149) holding a reagent fluid (114). The chemical reaction includes a nucleic acid sequencing on a wiregrid. Based on strong optical confinement of excitation light (110) and of cleavage light (112), the sequencing reaction can be read-out. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moieties. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked. In order to avoid overheating by cleavage light, the reagent fluid is circulated along the surface of the substrate (101).
    Type: Grant
    Filed: November 20, 2014
    Date of Patent: April 14, 2020
    Inventors: Pieter Jan Van Der Zaag, Anke Pierik
  • Patent number: 10620110
    Abstract: A sample analyzer with an optical detection device and a sample analysis method of the sample analyzer are disclosed. The optical detection device includes a fluid chamber, a light source and a light detector. The fluid chamber includes an illumination zone. An analyte flows through the illumination zone so as to form a sample stream. The light source illuminates the illumination zone to excite cell articles, reacted with a reagent, of the sample stream to emit a light signal. The light detector detects the fluorescent lights and transforms it into an electric signal. The light detector can include a silicon photomultiplier.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: April 14, 2020
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Xiansuan Du, Yong Dai
  • Patent number: 10620190
    Abstract: An automated microscope apparatus, comprises an outer housing having an external wall; optionally but preferably an internal wall in said housing, and configured to form a first compartment and a separate second compartment in said outer housing; a microscope assembly in said housing, preferably in said first compartment; and a microprocessor in said housing, preferably in said second compartment; and optionally but preferably a heat sink mounted on said housing external wall, preferably adjacent said second compartment, with said microprocessor thermally coupled to said heat sink and operatively associated with said microscope assembly.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: April 14, 2020
    Assignee: Advanced Animal Diagnostics, Inc.
    Inventors: Stefano Bresolin, David A. Calderwood, Tobias M. Heineck, David Newcomb, Chris Paul, Jaspar N> Pollard, Rodolfo R. Rodriguez, Demetris Young
  • Patent number: 10618931
    Abstract: Provided herein are methods for the synthesis of oligomeric compounds wherein removal of the 5?-terminal trityl group is performed at reduced temperature and lower pH relative to standard methods. In certain embodiments, the present methods provide detritylated oligomeric compounds having a reduced percentage of depurination relative to the same detritylated oligomeric compounds prepared using standard methods. In certain embodiments, the present methods provide detritylated oligomeric compounds with increased purity relative to the same detritylated oligomeric compounds prepared using standard methods. In certain embodiments, the present methods provide an increased rate of detritylation compared to standard methods.
    Type: Grant
    Filed: July 27, 2017
    Date of Patent: April 14, 2020
    Assignee: Ionis Pharmaceuticals, Inc.
    Inventor: Dana Chreng
  • Patent number: 10619192
    Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.
    Type: Grant
    Filed: April 8, 2015
    Date of Patent: April 14, 2020
    Inventors: Daniel T. Chiu, Jason E. Kreutz, Gloria S. Yen, Bryant S. Fujimoto
  • Patent number: 10613256
    Abstract: A biometric device includes an illuminating unit and an imaging module. The imaging module includes an optical angular selective structure and a sensing layer. The light selecting structure includes a micro lens array, a refractive layer and a light shielding layer. The refractive layer is disposed between the micro lens array and the light shielding layer. The micro lens array includes a plurality of lens unit units, and the light shielding layer has a plurality of light passing portions. The sensing layer defines multiple sensing regions which are spaced apart from each other. The light shielding layer is disposed between the refractive layer and the sensing layer. The sensing regions correspond to the light passing portions, respectively. An optical angular selective distance is defined between the light shielding layer and the sensing layer.
    Type: Grant
    Filed: December 27, 2017
    Date of Patent: April 7, 2020
    Inventors: Ding-Zheng Lin, Yu-Ling Hsu, Yaw-Ting Wu, Ping-Chen Chen