Measuring Or Testing For Antibody Or Nucleic Acid, Or Measuring Or Testing Using Antibody Or Nucleic Acid Patents (Class 435/287.2)
  • Patent number: 11447817
    Abstract: The inventions cover systems and methods for generation of emulsions having suitable clarity without requiring refractive index matching between emulsion components. Systems can include: a substrate including a set of openings; a reservoir facing the substrate at a first side and containing a sample fluid configured for droplet formation upon interacting with the set of openings of the substrate; and a collecting container facing the substrate at a second side and containing a set of fluid layers configured with a density gradient and suitable immiscibility characteristics. One or more components of the system(s) can support methods for emulsion generation, in relation to enabling interactions between multiple continuous phases and a dispersed droplet phase to generate clear emulsions. Applications of the inventions(s) can include performance of droplet-based digital PCR in an improved manner (e.g., without requiring implementation of correction factors based upon Poisson statistics).
    Type: Grant
    Filed: December 23, 2021
    Date of Patent: September 20, 2022
    Assignee: Enumerix, Inc.
    Inventors: Hei Mun Christina Fan, Eleen Yee Lam Shum, Janice Hoiyi Lai, Stephen P. A. Fodor
  • Patent number: 11446630
    Abstract: An integrated circuit includes a plurality of sensing pixels, each sensing pixel including a sensing film portion, a bio-sensing device configured to generate a first signal responsive to an electrical characteristic of the sensing film portion, a first switching device coupled between the bio-sensing device and a first signal path, a temperature-sensing device configured to generate a second signal responsive to a temperature of the sensing film portion, and a second switching device coupled between the temperature-sensing device and a second signal path.
    Type: Grant
    Filed: November 15, 2019
    Date of Patent: September 20, 2022
    Assignee: TAIWAN SEMICONDUCTOR MANUFACTURING COMPANY, LTD.
    Inventors: Tung-Tsun Chen, Yi-Shao Liu, Jui-Cheng Huang, Chin-Hua Wen, Felix Ying-Kit Tsui, Yung-Chow Peng
  • Patent number: 11446657
    Abstract: A microfluidic system comprising a chamber closed by movable elements and connected to at least one channel. The system has at least one structured component and at least one component attached to the structured component. The chamber is used such that the movable element can be moved into the chamber as well as out of the chamber by a movement of the movable element. Liquids or gases can be moved via one or more channels connected to the chamber by the movement and dispensed or received out of the structured component via a connection of the channel. A liquid reagent reservoir is connected to the pump chamber via the sample supply channel. Thus, the system can be used to receive, pump, dilute, mix, and dispense liquids or gases.
    Type: Grant
    Filed: November 16, 2017
    Date of Patent: September 20, 2022
    Assignee: MICROFLUIDIC CHIPSHOP GMBH
    Inventors: Christian Moche, Richard Klemm, Claudia Gärtner
  • Patent number: 11445943
    Abstract: Devices and methods are described herein for directly and accurately measuring sweat flow rates using miniaturized thermal flow rate sensors. The devices (100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500) include the flow rate sensors (220, 320, 420, 520, 620, 720, 820, 920, 1020, 1120, 1220, 1320, 1420) in or adjacent to a microfluidic component (230, 330, 430, 530, 630, 730, 830, 930, 1030, 1130, 1230, 1330, 1430, 1530) of a wearable sweat sensing device. The devices and methods optimize the sensitivity of the flow rate sensors, while minimizing the presence of noise, in order to accurately and directly measure sweat flow rates.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: September 20, 2022
    Assignee: University of Cincinnati
    Inventors: Jason Charles Heikenfeld, Michelle Hoffman, Mikel Larson, Nicholas Bailey, Andrew Beckman, Adam Hauke
  • Patent number: 11440013
    Abstract: A device is provided comprising a receiving chamber for receiving a patient's sample, a first reagent reservoir and a second reagent reservoir. The receiving chamber may be configured for receiving at least an end portion of a sample collecting element, with the patient's sample being collected by that sample collecting element. The first reagent reservoir may generally be configured to accommodate a liquid reagent which may include a lysing agent, i.e. the liquid may be a kind of a lysis buffer. Likewise, the second reagent reservoir may generally be configured to accommodate a dry reagent which may include magnetic particles. The device may further comprise at least one of the following two actuators, a first actuator for supplying a first reagent from the first reagent reservoir to the sample and a second actuator for supplying a second reagent from the second reagent reservoir to the resulting mixture.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: September 13, 2022
    Assignee: Koninklijke Philips N.V.
    Inventors: Matthias Irmscher, Toon Hendrik Evers, Marlieke Joan Overdijk, Thomas Johannes Van Gijsel, Stefan Johannes Hendrikus Toonen
  • Patent number: 11440005
    Abstract: The present disclosure relates to a device capable of adjusting the pH of a sample and provides a device comprising microbeads capable of adjusting the pH of a sample to a predetermined value. The device comprises microbeads enabling adjustment to different pH values in each of a plurality of channels, and enables adjustment of the pH of the sample to a plurality of different pH values simultaneously upon injection of the sample.
    Type: Grant
    Filed: August 30, 2019
    Date of Patent: September 13, 2022
    Assignee: LG CHEM, LTD.
    Inventors: Byung Hyun Park, Byoung Hyoun Kim, Su Youn Han
  • Patent number: 11440003
    Abstract: A method of manufacturing a device useable in DNA or genome sequencing comprises disposing pairs of electrodes on a substrate, the electrodes within each pair separated by a nanogap; depositing a resist layer over the electrodes; patterning the resist layer to create an exposed region on each electrode at or near each nanogap; roughening the electrode surface within each exposed region using various methods; and exposing the exposed regions to biomolecules, wherein one biomolecule bridges each nanogap of each electrode pair, with each end of each biomolecule bound to the electrodes at each exposed region.
    Type: Grant
    Filed: June 25, 2020
    Date of Patent: September 13, 2022
    Assignee: ROSWELL BIOTECHNOLOGIES, INC.
    Inventors: Chulmin Choi, Sungho Jin, Paul W. Mola, Barry L. Merriman
  • Patent number: 11421127
    Abstract: Random copolymers, crosslinked thin films of the random copolymers and cell culture substrates comprising the crosslinked thin films are provided. Also provided are methods of making and using the copolymers, thin films and substrates. The copolymers are polymerized from glycidyl methacrylate monomers and vinyl azlactone monomers. The crosslinked thin films are substrate independent, in that they need not be covalently bound to a substrate to form a stable film on the substrate surface.
    Type: Grant
    Filed: November 27, 2019
    Date of Patent: August 23, 2022
    Inventors: Padma Gopalan, William L. Murphy, Samantha Kelly Schmitt
  • Patent number: 11421267
    Abstract: The invention features compositions and methods that are useful for the measurement of the quantity of a nucleic acid target in a sample.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: August 23, 2022
    Assignee: Accugenomics, Inc.
    Inventor: Tom B. Morrison
  • Patent number: 11413616
    Abstract: Described herein are systems relating to a continuous-flow instrument that includes all necessary components for digital droplet quantification without the need to introduce key reagents or collect and transfer droplets between stages of instrument operation. Digital quantification can proceed without any additional fluid or consumable handling and without exposing fluids to risk of external contamination.
    Type: Grant
    Filed: May 15, 2019
    Date of Patent: August 16, 2022
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Cody Youngbull, Andrew Hatch, Andrew Larsen, Tathagata Ray, Matthew Underhill
  • Patent number: 11413620
    Abstract: Instrument for performing a diagnostic test on a fluidic cartridge A cartridge reader is for carrying out a diagnostic test on a sample contained in a fluidic cartridge inserted into the reader. The fluidic cartridge comprises a fluidic layer comprising at least one sample processing region, at least one collapsible blister containing a liquid reagent, a pneumatic interface, an electrical interface and at least one mechanical valve. The reader comprises a housing; an upper clamp occupying a fixed position relative to the reader, and a lower clamp, movable relative to the first clamp, wherein the upper clamp and the lower clamp define a cartridge receiving region therebetween. The reader comprises a thermal module comprised in the lower clamp, wherein the thermal module comprises at least one thermal stack for heating the at least one sample processing region of the cartridge inserted into the reader.
    Type: Grant
    Filed: May 8, 2020
    Date of Patent: August 16, 2022
    Assignee: Binx Health Limited
    Inventors: Ben Arlett, Tom Edwards
  • Patent number: 11408004
    Abstract: The present invention relates to antisense oligonucleotide that modulate the expression of and/or function of a Sirtuin (SIRT), in particular, by targeting natural antisense polynucleotides of a Sirtuin (SIRT). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of Sirtuins (SIRT)s.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: August 9, 2022
    Assignee: CURNA, INC.
    Inventors: Joseph Collard, Olga Khorkova Sherman, Carlos Coito, Belinda De Leon
  • Patent number: 11396015
    Abstract: Provided herein are methods for processing and/or detecting a sample. A method can comprise providing a barrier between a first region and a second region, wherein the first region comprises the sample, wherein the barrier maintains the first region at a first atmosphere that is different than a second atmosphere of the second region, wherein a portion of the barrier comprises a fluid in coherent motion; and using a detector at least partially contained in the first region to detect one or more signals from the sample while the first region is maintained at the first atmosphere that is different than the second atmosphere of the second region. The portion of the barrier comprising fluid may have a pressure lower than the first atmosphere, the second atmosphere, or both.
    Type: Grant
    Filed: October 28, 2019
    Date of Patent: July 26, 2022
    Assignee: Ultima Genomics, Inc.
    Inventors: Nathan Beckett, Nathan Caswell
  • Patent number: 11389793
    Abstract: The present invention relates to a method for producing a microfluidic device, in particular, a sol-gel method for producing a microfluidic device in hybrid silica glass. The invention also relates to a microfluidic device obtainable by the method as described above and to microfluidic device in hybrid silica glass comprising at least one microchannel having a depth of at least 1 ?m, preferably between 1 ?m and 1 mm, and more preferably between 10 and 100 ?m.
    Type: Grant
    Filed: September 8, 2017
    Date of Patent: July 19, 2022
    Assignees: TOTAL SA, ECOLE NORMALE SUPERIEURE DE LYON, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE-CNRS-, UNIVERSITE CLAUDE BERNARD LYON 1
    Inventors: Michael Levant, Denis Bartolo, Stéphane Parola, Denis Chateau
  • Patent number: 11391719
    Abstract: Devices for sequencing linear biomolecules (e.g., DNA, RNA, polypeptides, proteins, and the like) using quantum tunneling effects, and methods of making and using such devices, are provided. A nanofabricated device can include a small gap formed by depositing a thin film between two electrodes, and subsequently removing the film using an etching process. The width of the resulting gap can correspond with the size of a linear biomolecule such that when a part of the biomolecule (e.g., a nucleobase or amino acid) is present in the gap, a change in tunneling current, voltage, or impedance can be measured and the part of the biomolecule identified. The gap dimensions can be precisely controlled at the atomic-scale by, for example, atomic layer deposition (ALD) of the sacrificial film. The device can be made using existing integrated circuit fabrication equipment and facilities, and multiple devices can be formed on a single chip.
    Type: Grant
    Filed: December 21, 2017
    Date of Patent: July 19, 2022
    Assignee: Roche Sequencing Solutions, Inc.
    Inventor: Steven Henck
  • Patent number: 11390836
    Abstract: A micropillar and microwell chip and methods of studying cellular environments using micropillar and microwell chips is disclosed. The micropillar chip may include at least one micropillar with a pillar-microwell. The microwell chip may include at least one microwell with an upper and a lower microwell. A perfusion channel chip that may be integrated with a micropillar chip is also disclosed. The perfusion channel chip may include a channel, a pillar-insertion hole, a membrane cassette, and a reservoir well.
    Type: Grant
    Filed: November 17, 2017
    Date of Patent: July 19, 2022
    Assignee: Cleveland State University
    Inventor: Moo-Yeal Lee
  • Patent number: 11377684
    Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: July 5, 2022
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Duc Do, Claudia Litterst, Dianna Maar
  • Patent number: 11358146
    Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir through which fluid flow is controlled along a fluid path; and wherein the method includes: capturing a population of particles into the array of wells in single-particle format; releasing, from the particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.
    Type: Grant
    Filed: May 28, 2021
    Date of Patent: June 14, 2022
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Vishal Sharma, Priyadarshini Gogoi, William Chow, Austin Payne, Kyle Gleason, Brian Boniface, John Connolly, Sam Tuck
  • Patent number: 11359231
    Abstract: A method for sensing an analyte uses tethered particle motion. A functionalized particle has a first state in which the functionalized particle is bound to the surface and a second state in which the functionalized particle is not bound to the surface, where the functionalized particle switches between the first and second states depending on the presence and absence of the analyte, thereby changing motion characteristics of the functionalized particle depending on the presence of the analyte. A spatial coordinate parameter of the functionalized particle is measured by a detector, and a processor determines the presence/concentration of the analyte from changes in the measured spatial coordinate parameter.
    Type: Grant
    Filed: December 21, 2019
    Date of Patent: June 14, 2022
    Assignee: Technische Universiteit Eindhoven
    Inventors: Menno Willem José Prins, Maarten Merkx, Leonardus Josephus van Ijzendoorn, Peter Zijlstra, Emilius Willem Adriaan Visser, Max Rose-Marie Wilhelmus Scheepers
  • Patent number: 11346798
    Abstract: The present invention relates to a diagnostic device and methods of using the same for diagnostic assays for monitoring the presence of biological samples wherein the device allows for the determination of at least two assay components on one sensor. More specifically, the invention relates to a multi-marker electrochemical impedance spectroscopy sensor comprising a plurality of molecular recognition elements wherein the sensor comprises multiple different molecular recognition element types that are tuned in a manner that alters the frequency of the molecular recognition element type such that it is at a detectably different frequency to the frequency of other molecular recognition element types on the same sensor.
    Type: Grant
    Filed: July 12, 2011
    Date of Patent: May 31, 2022
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Jeffrey LaBelle, Ugur Demirok
  • Patent number: 11338285
    Abstract: A technique relates to a fluidic cell configured to hold a nanofluidic chip. A first plate is configured to hold the nanofluidic chip. A second plate is configured to fit on top of the first plate, such that the nanofluidic chip is held in place. The second plate has at least one first port and at least one second port. The second plate has an entrance hole configured to communicate with an inlet hole of the nanofluidic chip. The second port is angled above the first port, such that the first port and second port intersect to form a junction. The second port is formed to have a line-of-sight to the entrance hole, such that the second port is configured to receive input for extracting air trapped at a vicinity of the entrance hole.
    Type: Grant
    Filed: May 31, 2019
    Date of Patent: May 24, 2022
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Michael A. Pereira, Joshua T. Smith, Benjamin H. Wunsch
  • Patent number: 11340210
    Abstract: The embodiments disclose a method including functionalizing a biosensor with a biologic analytical target prior to installation into a detection cartridge, depositing a test subject bodily fluid test sample onto the biosensor surface, inserting the detection cartridge into a portable detection cartridge reader, measuring the electrical impedance of the bodily fluid test sample across biosensor energized electrodes, providing algorithms for analyzing measured electrical impedance data of the bodily fluid test sample obtained in the detection cartridge, identifying and determining the presence of biologic analytical target molecules in the bodily fluid test sample, and transmitting results of the test results to the test subject.
    Type: Grant
    Filed: May 18, 2021
    Date of Patent: May 24, 2022
    Inventors: Matthew Hummer, Gregory J. Hummer
  • Patent number: 11335438
    Abstract: A method for detecting false positive variant calls in a next generation sequencing analysis pipeline involves obtaining a plurality of read pileup windows associated with a first sample genome. The method also involves obtaining, for each reference nucleotide position represented in the plurality of read pileup windows, a label indicating that the reference nucleotide position is either (i) a known variant or (ii) a non-variant. The method further involves training a neural network based on data indicative of the plurality of read pileup windows and the labels. Additionally, the method involves receiving a read pileup window associated with a second sample genome. Further, the method involves determining, using the trained neural network, a likelihood that the read pileup window associated with the second sample genome represents a variant.
    Type: Grant
    Filed: April 18, 2017
    Date of Patent: May 17, 2022
    Assignee: Verily Life Sciences LLC
    Inventors: Mark Andrew DePristo, Ryan Poplin
  • Patent number: 11324804
    Abstract: The present invention relates to the field of medicine and provides compositions and kits-of-parts comprising a CD6 product and Imipenem, in particular for their use in a therapeutic and/or preventive method of treatment, in a mammal including a human, of an infectious disease, or of an inflammatory condition related to an infectious disease, or of an inflammatory disease related to the presence of a product derived from an infectious agent.
    Type: Grant
    Filed: November 17, 2017
    Date of Patent: May 10, 2022
    Assignee: Sepsia Therapeutics, S.L.
    Inventors: Francisco Lozano Soto, Mario Martinez Florensa
  • Patent number: 11320348
    Abstract: A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: May 3, 2022
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Yu-Heng Cheng, Setareh Duquette, Lisa A. Jones, Chih-Ching Lin, Javier Andres Perez-Sepulveda
  • Patent number: 11319588
    Abstract: This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.
    Type: Grant
    Filed: March 8, 2019
    Date of Patent: May 3, 2022
    Assignee: MGI Tech Co., Ltd.
    Inventors: Rongqin Ke, Snezana Drmanac, Radoje Drmanac, Guangyang Cai, Matthew Callow
  • Patent number: 11312952
    Abstract: The present invention is related to a method for recovering two or more genes, or gene products, or cDNAs, encoding for an immunoreceptor having two or more subunits, which two or more genes, or gene products, are comprised in a given source cell. The invention is further related to a method of creating a library of expressor cells, in which library each cell is capable of expressing two or more genes, or gene products, encoding for the subunits of the immunoreceptor. The invention is further related to a method of screening a library of expressor cells as created according to the above method, for one cell that expresses an immunoreceptor that has specificity for a given target molecule.
    Type: Grant
    Filed: February 13, 2015
    Date of Patent: April 26, 2022
    Assignee: Memo Therapeutics AG
    Inventors: Martin Held, Christoph Esslinger
  • Patent number: 11307195
    Abstract: An impact blood splatters generation experimental apparatus capable of measuring an applied force includes: a hammer including a rod-shaped body portion, a head portion formed at one end of the body portion, and a striking portion formed on one side of the head portion; a housing receiving a certain amount of blood thereon and including a striking surface on which the striking portion may strike; a tensile force gauge attached to an outer surface of the housing; an elastic member having one end connected to the body portion and the other end connected to the tensile force gauge; and a display unit displaying a force applied to the hammer, the force being measured by the tensile force gauge, when a user lifts the hammer to strike the striking surface.
    Type: Grant
    Filed: December 10, 2019
    Date of Patent: April 19, 2022
    Assignee: REPUBLIC OF KOREA(MANAGEMENT: NATIONAL FORENSIC SERVICE DIRECTOR, MINISTRY OF PUBLIC ADMINISTRATION AND SECURITY)
    Inventors: Nam Kyu Park, Jae Mo Goh, Jin Pyo Kim, Young Il Seo, Eun Ah Joo, Je Hyun Lee, Sang Yoon Lee, Dong A Lim, Kyung Mi Kim
  • Patent number: 11293919
    Abstract: The invention includes a method of assaying an analyte in a sample in a portable, handheld microfluidic reader. The method includes the steps of: inserting the sample in the reader; capturing the analyte with a first antibody having a DNA tag attached thereto; capturing the analyte in the sample with a second antibody attached to a surface or having a magnetic nanoparticle (MNP) attached thereto; where a sandwich including the magnetic nanoparticle, first and second antibodies, the analyte and the DNA tag is formed; replicating the DNA tag using isothermal amplification to a predetermined amount of DNA tags detectable by a detector sufficient to overcome the minimal mass sensitivity limitations of the detector; and measuring the amount of replicated DNA tags using the detector. The invention also includes an apparatus or handheld portable field microfluidic reader in which the method is performed.
    Type: Grant
    Filed: December 13, 2019
    Date of Patent: April 5, 2022
    Assignee: Autonomous Medical Devices Inc.
    Inventors: Josh Shachar, Roger Kornberg
  • Patent number: 11295225
    Abstract: Passive and actives approaches to mitigating the effects of spin-bath polarization are described and illustrated. Such may, for example, include at least partially depolarizing the spin-bath polarization, for instance by: performing an annealing cycle by the quantum processor to generate a final state of a qubit of the quantum processor; flipping the final state of the qubit of the quantum processor to an opposite state; and latching the qubit in the opposite state for a predetermined duration.
    Type: Grant
    Filed: July 6, 2018
    Date of Patent: April 5, 2022
    Assignee: D-WAVE SYSTEMS INC.
    Inventors: Emile M. Hoskinson, Trevor Michael Lanting
  • Patent number: 11293057
    Abstract: The invention provides an alternating current electrospray technology that can generate micron sized droplets in oil at very high throughput for emulsion or digital PCR (Polymerase Chain Reaction). This technology outperforms the throughput of the current gold standard in droplet generation using flow-focusing technology by at least a factor of 100. The design is simple and can generate a billion to a trillion monodispersed droplets in about one hour. This is much faster than flow-focusing which is limited to a few million droplets per hour. The droplet size and generation rate can also be easily adjusted by changing the voltage of the AC electric field. The range of produced droplet sizes is about 1-100 microns, wherein the droplets are monodispersed in size.
    Type: Grant
    Filed: May 9, 2017
    Date of Patent: April 5, 2022
    Assignee: University of Notre Dame du Lac
    Inventors: Hsueh-Chia Chang, David B. Go, Zdenek Slouka, Satyajyoti Senapati, Yongfan Men, Zehao Pan
  • Patent number: 11291995
    Abstract: Selectively vented biological assay devices and methods of performing biological assays with such devices are provided herein. Disclosed devices include (a) a sample receiving cartridge comprising a sample inlet and one or more reaction chambers and (b) a selective venting element having passively tunable porosity. The methods include controlling fluid flow within the subject devices with the selective venting element.
    Type: Grant
    Filed: March 14, 2017
    Date of Patent: April 5, 2022
    Assignee: Lucira Health, Inc.
    Inventors: Debkishore Mitra, Frank B. Myers, III, John Robert Waldeisen, Ivan Krastev Dimov
  • Patent number: 11273444
    Abstract: Disclosed herein are microfluidic devices and systems for amplifying and detecting a target polynucleotide, comprising: one or more wells for receiving one or more substrates; a droplet generation channel in fluid communication with the one or more wells, wherein the microfluidic channel is adapted to generate droplets; and a chamber in fluid communication with the droplet generation channel, and adapted to collect droplets generated by the droplet generation channel, and further adapted to perform nucleic acid amplification in droplets, and further adapted to detect light signal from droplets. Also disclosed are methods of using the same.
    Type: Grant
    Filed: November 15, 2019
    Date of Patent: March 15, 2022
    Assignee: Precigenome, LLC
    Inventors: Chen Li, Yunfeng Ling, Cifeng Fang, Yaqi Wang, Yu Liu
  • Patent number: 11268143
    Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.
    Type: Grant
    Filed: May 5, 2021
    Date of Patent: March 8, 2022
    Assignee: Ultima Genomics, Inc.
    Inventors: Nathan Beckett, Gilad Almogy, Nathan Caswell, Jacob A. Wolf, Kristopher Barbee, Denis Pristinski, Mark Pratt, Gene Polovy, Osip Schwartz, Stephanie Kubecka, Steven Menchen, Joseph Anthony, Jose Martin Sosa, Phillip You Fai Lee
  • Patent number: 11262367
    Abstract: Analyte collection and testing systems and methods, and more particularly to disposable oral fluid collection and testing systems and methods. Described herein are methods and apparatuses to achieve significant improvements in the detection of fluorescence signals in the reader.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: March 1, 2022
    Assignee: Evanostics LLC
    Inventors: Ashutosh Shastry, David Piehler, Hardeep Sanghera, Michael Gluzczack, Pranav Chopra, Yun-Pei Chang, Ameya Kantak
  • Patent number: 11260393
    Abstract: A digital PCR device using centrifugal force. The present disclosure comprises: sample dish on which a microwell film having formed microwells is mounted; a door unit for inputting a sample while rotating the sample dish, and controlling the temperature of the sample which has been fractionated in the microwells by means of the centrifugal force and thus performing a PCR process; and a scan head unit for reading a fluorescent signal while rotating the sample which has been amplified in the microwells during the PCR process.
    Type: Grant
    Filed: January 4, 2018
    Date of Patent: March 1, 2022
    Inventors: Sung Woon Lee, Ho Chul Nam
  • Patent number: 11262307
    Abstract: Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducial markers may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducial markers may include features and materials that are provided on or in the support of a patterned array and that return at least a portion of incident light by reflection. The fiducial markers may form gratings or other encoding configurations that assist in image processing, alignment, or other aspects of processing of the patterned array.
    Type: Grant
    Filed: January 31, 2018
    Date of Patent: March 1, 2022
    Assignee: Illumina, Inc.
    Inventors: John S. Vieceli, Alex Nemiroski, Paul Belitz, Robert Langlois, M. Shane Bowen, Danny Yuan Chan, Bala Murali K. Venkatesan, Hui Han, Kevan Samiee, Stephen Tanner
  • Patent number: 11255771
    Abstract: A photon-accounting system for use with a flow cytometry system is disclosed which includes a signal shaping sub-system, including a differentiator configured to generate a differentiated output of photodiode signals into corresponding zero-crossings each associated with one of the received photons, a comparator configured to receive the differentiated signal and compare to a threshold to thereby generate a comparator output digital signal associated with the crossing of the differentiated signal about the threshold, a front-end synchronization system adapted to receive and synchronize the comparator generated digital signal to a clock, thereby generate synchronized photon data with the clock and associated with the asynchronized photodiode signal, and a timestamping system adapted to receive the synchronized data as a bit stream and generate a timestamp associated with each photon data.
    Type: Grant
    Filed: June 22, 2020
    Date of Patent: February 22, 2022
    Assignee: CAPTL LLC
    Inventors: Masanobu Yamamoto, J. Paul Robinson, Keegan Hernandez
  • Patent number: 11247205
    Abstract: Surface-modified glass and polymer membrane interfaces form high-electrical resistance seals that can be used in microfluidic valves and array devices tailored for electrophysiological measurements. The incorporation of high seal resistance valves into the array device allows only the desired electrophysiological signal to be detected by a patch clamp amplifier, enabling parallel experiments with one patch clamp amplifier, which can greatly improve the cost efficiency. To achieve the desired high seal resistance, surface modification was performed on the glass components to increase the interaction between the glass and the membrane surfaces. The valves exhibit seal resistance of >500 G? after modification, which is 100× higher than reported for unmodified valves.
    Type: Grant
    Filed: April 16, 2018
    Date of Patent: February 15, 2022
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA
    Inventors: Xuemin Wang, Christopher Baker, Craig A. Aspinwall
  • Patent number: 11247210
    Abstract: The present disclosure relates to a tempering block module for the thermal treatment of samples, comprising: a tempering block; and an ejection mechanism for lifting reaction vessels off the tempering block, the ejection mechanism including first and second ejection plungers that are movably mounted in the tempering block module perpendicular to the tempering block from a first position, retracted into the tempering block module, into a second position, extended out of the tempering block module, and wherein the tempering block module includes a first plunger drive connected to the first ejection plunger for driving the movement of the first ejection plunger and a second plunger drive, different from the first plunger drive, connected to the second ejection plunger for driving the movement of the second ejection plunger from the first to the second position or from the second to the first position.
    Type: Grant
    Filed: October 2, 2019
    Date of Patent: February 15, 2022
    Assignee: Biometra GmbH
    Inventors: Eggert Appel, Janek Junghans, Konstantin Missal
  • Patent number: 11247211
    Abstract: The present disclosure relates to a device for the thermal treatment of samples, including: a base unit with a receiving region; a cover for closing the receiving region, the cover movable from a first, open position into a second, closed position; at least one connecting element connected to the cover; and a cover drive disposed in the base unit and coupled to the at least one connecting element as to drive a movement of the cover such that, during the movement from an open into a closed position, the cover is initially brought from the open position into a third position, in which the cover extends parallel to and spaced from the receiving region, and such that the cover is further moved from the third position in a direction of a shared normal until the cover has reached the closed position.
    Type: Grant
    Filed: October 2, 2019
    Date of Patent: February 15, 2022
    Assignee: Biometra GmbH
    Inventors: Eggert Appel, Janek Junghans, Konstantin Missal
  • Patent number: 11242557
    Abstract: Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: February 8, 2022
    Assignee: OMNIOME, INC.
    Inventors: Morassa Mohseni Middleton, Mark C. Wallen, Pinar Iyidogan, Michael James Schmidt, Brittany A. Rohrman, Ying Lin Liu, Fabian Block, Arnold Oliphant
  • Patent number: 11241692
    Abstract: A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: February 8, 2022
    Assignee: MGI Tech Co., LTD.
    Inventors: Wei Ma, Xun Xu, Jiabo Wu, Ming Ni, Dong Wei, Jiansheng Tang
  • Patent number: 11235324
    Abstract: The present disclosure is drawn to temperature-cycling microfluidic devices. In one example, a temperature-cycling microfluidic device can include a driver chip having a top surface and a heat exchange substrate having a top surface coplanar with the top surface of the driver chip. A fluid chamber can be located on the top surface of the driver chip. A first and second microfluidic loop can have fluid driving ends and fluid outlet ends connected to the fluid chamber and can include portions thereof located on the top surface of the heat exchange substrate. A first and second fluid actuator can be on the driver chip. The first and second fluid actuators can be associated with the fluid driving ends of the first and second microfluidic loops, respectively, to circulate fluid through the first and second microfluidic loops.
    Type: Grant
    Filed: November 22, 2017
    Date of Patent: February 1, 2022
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Adam Higgins, Alexander Govyadinov, Michael W. Cumbie
  • Patent number: 11231404
    Abstract: A device and method for collecting, analyzing, and identifying chemical and biological samples in solid or liquid form is disclosed. The analysis compares the color change of Colorimetric Sensor Arrays (CSAs) over time with images for known materials/compounds contained in a library. The device can be used as a handheld or standalone device and integrates the sampling and detecting functions of the prior art into a single device that analyzes and identifies the sample without destroying it. The inventive volatile organic compound (VOC) technology device is also unique in that it relies on a comparison to a proprietary compound library that is supported by lab data. This compound library identifies the unique dye signature combinations that provide very accurate classification of a wide variety of chemical and biological agents.
    Type: Grant
    Filed: August 28, 2019
    Date of Patent: January 25, 2022
    Assignee: The United States of America as Represented by the Secretary of the Army
    Inventors: Peter A Emanuel, Calvin W Chue, Gregory A Thompson, Colin W Graham, Aleksandr E Miklos, Jacob J Shaffer
  • Patent number: 11229910
    Abstract: Described herein are microfluidic devices and systems for high density cell culture and/or high throughput cell assays. Methods of using the same are also provided herein. In some embodiments, the microfluidic devices and systems described herein provide rapid and automated trapping of single embryos in ordered arrays.
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: January 25, 2022
    Assignees: President and Fellows of Harvard College, Tufts University
    Inventors: Richard Novak, Donald E. Ingber, Michael Levin, Rachelle Prantil-Baun
  • Patent number: 11229906
    Abstract: A lateral flow assay (LFA) device includes a sample port that holds the sample fluid before a hole is made in a wall of the sample port. The LFA device includes a cap with a sealer. The sealer fits inside the sample port after the sample port receives the sample fluid, forms a compartment for holding a predetermined volume of sample fluid between the sample port's wall and the sealer, and pushes any amount of sample fluid in excess of the predetermined volume out of the compartment after the cap is fitted inside the sample port. The LFA device includes a breaker with a tip to make a hole in the wall of the sample port causing the sample fluid held inside the compartment to be applied to the capillary pad after the start of a test.
    Type: Grant
    Filed: June 21, 2021
    Date of Patent: January 25, 2022
    Inventors: Mehdi Hatamian, Mehrtash Ghalebi
  • Patent number: 11224874
    Abstract: A field portable diagnostic apparatus uses a rotatable disk in which a microfluidic circuit is defined. The microfluidic circuit includes a centrifugal separation chamber receiving a sample to stratify the sample. A magnetic bead holding chamber is communicated to a mixing chamber, where mass amplifying functionalized magnetic-nanoparticles, held in a buffer solution and contained in the magnetic bead holding reservoir communicated to mixing chamber, are mixed with the separated fluid delivered to mixing chamber from the separation chamber. The functionalized magnetic nanoparticles conjugate with a target analyte in the sample. A magnet in proximity to a SAW chamber including a SAW detector draws the functionalized magnetic nanoparticles toward antibodies immobilized on the SAW sensor surface A wash reservoir is communicated to the SAW sensor chamber, and a cleanup/waste reservoir is communicated to the SAW chamber for receive fluid after it has passed through the SAW chamber.
    Type: Grant
    Filed: February 25, 2019
    Date of Patent: January 18, 2022
    Assignee: Autonomous Medical Devices Inc.
    Inventors: Josh Yehoshua Shachar, Sam Gurley, Aaron Cipriano, Peter Yin, Rob Purnell, Marc Rocklinger, Ming Petrullo, Stelica Stelea
  • Patent number: 11221319
    Abstract: A device and method for collecting, analyzing, and identifying chemical and biological samples in solid or liquid form is disclosed. The analysis compares the color change of Colorimetric Sensor Arrays (CSAs) over time with images for known materials/compounds contained in a library. The device can be used as a handheld or standalone device and integrates the sampling and detecting functions of the prior art into a single device that analyzes and identifies the sample without destroying it. The inventive volatile organic compound (VOC) technology device is also unique in that it relies on a comparison to a proprietary compound library that is supported by lab data. This compound library identifies the unique dye signature combinations that provide very accurate classification of a wide variety of chemical and biological agents.
    Type: Grant
    Filed: August 28, 2019
    Date of Patent: January 11, 2022
    Assignee: The United States of America as Represented by the Secretary of the Army
    Inventors: Peter A Emanuel, Calvin W Chue, Gregory A Thompson, Colin W Graham, Aleksandr E Miklos, Jacob J Shaffer
  • Patent number: 11215550
    Abstract: A sample analyzer with an optical detection device and a sample analysis method of the sample analyzer are disclosed. The optical detection device includes a fluid chamber, a light source and a light detector. The fluid chamber includes an illumination zone. An analyte flows through the illumination zone so as to form a sample stream. The light source illuminates the illumination zone to excite cell articles, reacted with a reagent, of the sample stream to emit a light signal. The light detector detects the fluorescent lights and transforms it into an electric signal. The light detector can include a silicon photomultiplier.
    Type: Grant
    Filed: December 29, 2020
    Date of Patent: January 4, 2022
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Xiansuan Du, Yong Dai