Means For Separation Or Recovery Of A Microorganism From Culture Media Patents (Class 435/308.1)
  • Patent number: 10976258
    Abstract: The invention relates to a porous planar cell capture system for use in determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using such a cell capture system. The cell capture system contains a fluid permeable, planar membrane adopted to retain cells thereon, a fluid permeable support member that supports the membrane, and an optional register associated with the membrane.
    Type: Grant
    Filed: February 25, 2019
    Date of Patent: April 13, 2021
    Assignees: Charles River Laboratories, Inc., ReaMetrix Inc.
    Inventors: Norman R. Wainwright, Bala S. Manian, Eric Stimpson, Brian J. Kolonia, Robert K. Kolonia
  • Patent number: 9329109
    Abstract: A cell processing apparatus includes a storage container that contains liquid L including a biological sample; a filter that prevents a first cell C1 in the biological sample from passing and allows a second cell C2 having a smaller diameter than that of the first cell C1 to pass; and a filtration cylinder for separating, in the storage container and via the filter, the liquid L into a first liquid L1 mainly including the first cell C1 and a second liquid L2 mainly including the second cell C2. A measurement target cell filtered by the filter among other cells can be easily collected.
    Type: Grant
    Filed: March 6, 2013
    Date of Patent: May 3, 2016
    Assignee: Sysmex Corporation
    Inventors: Masakazu Fukuda, Hironori Kobayashi, Junyi Ding, Ryuichiro Ebi, Koki Tajima
  • Patent number: 9174213
    Abstract: An apparatus for passive sorting of microdroplets including a main flow channel, a flow stream of microdroplets in the main flow channel wherein the microdroplets have substantially the same diameter and wherein the flow stream of microdroplets includes first microdroplets having a first degree of stiffness and second microdroplets having a second degree of stiffness wherein the second degree of stiffness is different than the first degree of stiffness. A second flow channel is connected to the main flow channel for the second microdroplets having a second degree of stiffness. A separator separates the second microdroplets having a second degree of stiffness from the first microdroplets and directs the second microdroplets having a second degree of stiffness into the second flow channel.
    Type: Grant
    Filed: February 24, 2015
    Date of Patent: November 3, 2015
    Assignees: Lawrence Livermore National Security, LLC, The Regents of the University of California
    Inventors: Neil Reginald Beer, Abraham P. Lee, Andrew C. Hatch, Jeffrey S. Fisher
  • Publication number: 20150147811
    Abstract: The invention relates to a method for separating off a liquid supernatant from cells, comprising the steps: providing a mixture of the cells with a liquid, charging a first filter housing with the mixture, wherein in the filter housing a filter having a pore size of between 4 ?m and 50 ?m is provided on a flat base surface pierced in a sieve manner and the walls of the filter housing are connected so as to seal with the flat base surface that is pierced in a sieve manner, applying a differential pressure of at least 0.5 bar on the mixture, as a result of which the liquid portion of the mixture is forced through the filter and a filter cake containing cells remains in the filter housing, and removal of the filtered liquid.
    Type: Application
    Filed: July 19, 2013
    Publication date: May 28, 2015
    Inventors: Grosse Thomas, Niederkrüger Holger, Schaaf Andreas
  • Publication number: 20150125948
    Abstract: An acoustophoresis device made up of modular components is disclosed. Several modules are disclosed herein, including ultrasonic transducer modules, input/output modules, collection well modules, and various connector modules. These permit different systems to be constructed that have appropriate fluid dynamics for separation of particles, such as biological cells, from a fluid.
    Type: Application
    Filed: November 5, 2014
    Publication date: May 7, 2015
    Inventors: Bart Lipkens, Jason Barnes, Dane Mealey, Walter M. Presz, JR., Stanley Kowalski, III, Louis Masi, Thomas J. Kennedy, III
  • Publication number: 20150111241
    Abstract: An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet.
    Type: Application
    Filed: April 17, 2013
    Publication date: April 23, 2015
    Inventors: Allison Hubel, Jacob Hanna
  • Publication number: 20150110763
    Abstract: A system is disclosed for washing a selected component and removing the selected component from a wash material. The selected component may include red blood cells that are washed to remove a rejuvenating solution. The red blood cells may then be removed with various systems, such as a standing acoustic wave system from the wash solution. Pumps and flow restrictors that provide steady flow from pumps that generate pulsed flow are also disclosed.
    Type: Application
    Filed: October 21, 2014
    Publication date: April 23, 2015
    Inventor: Michael D. Leach
  • Publication number: 20150079582
    Abstract: A bismuth-containing concentration agent for microorganisms is provided. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.
    Type: Application
    Filed: March 4, 2013
    Publication date: March 19, 2015
    Inventors: Manjiri T. Kshirsagar, Evan Koon Lun Yuuji Hajime, Andrew W. Rabins
  • Patent number: 8969021
    Abstract: The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: March 3, 2015
    Assignee: AVIVA Biosciences Corporation
    Inventors: Ping Lin, Douglas T. Yamanishi, George Walker, Junquan Xu, Mingxian Huang, Guoliang Tao, Lei Wu, Xiaobo Wang, Joe Ouyang, Jing Cheng, Jia Xu
  • Patent number: 8969074
    Abstract: The present invention relates to an electromagnetic bioaccelerator for obtaining biomass by simulating environmental marine conditions, comprising at least the following elements: octagonal biomass converters (1), seawater reserve tanks (3), particle filters (4), UV light filters (5), feedback and mixture tanks (6), pressurization feed tanks (8), manometers (9), pressure controllers (10), buffer tanks (11), expansion tanks with a safety valve (12), heat exchangers (13), temperature control thermostats (14), recycled water feedback tanks (15), reinjection pumps (16), centrifuges for separating the biomass from the water (17), desuperheaters (18); control panels (25), recirculation pumps (26), densimeters (27), biomass mechanical extraction systems by means of centrifugation (32) and biomass accumulation tanks (33).
    Type: Grant
    Filed: December 14, 2007
    Date of Patent: March 3, 2015
    Assignee: Bio Fuel Systems, S.L.
    Inventors: Bernard A. J. Stroïazzo-Mougin, Cristian Gomis Catala
  • Patent number: 8969075
    Abstract: A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway.
    Type: Grant
    Filed: December 18, 2008
    Date of Patent: March 3, 2015
    Assignee: University of Massachusetts
    Inventor: Andrew H. Fischer
  • Patent number: 8969073
    Abstract: The invention describes an appliance and a method, with the help of which specific bio-particles, but also dissolved bio-molecules can be recognized in and separated from fluids making use of suitable carriers and known immobilization methods. The appliance can be used both discontinuously and also for direct and continuous treatment of fluids. Fields of application of the invention are animals, bio-technology (including biological research) and medicinal diagnostics. Areas of application of the invention comprise, among others, therapy of humans, in particular direct treatment of blood.
    Type: Grant
    Filed: January 30, 2007
    Date of Patent: March 3, 2015
    Assignee: pluriSelect GmbH
    Inventor: Hans Werner Heinrich
  • Patent number: 8968681
    Abstract: A method and device is provided for collecting filterable material to perform assays on a filtration apparatus by applying negative-pressure to the inlet chamber of the filtration apparatus which is independent of negative or positive pressure applied to its fluid path. The negative-pressure applied to the inlet chamber serves to actively aspirate fluid and promote flow into the inlet chamber via the fluid pathway. The negative-pressure applied to the inlet chamber facilitates the use of either negative or positive-pressure based fluid-filtration approaches. Use of the invention improves the functionality and scope of applications that can be performed with enclosed filtration apparatuses.
    Type: Grant
    Filed: October 20, 2009
    Date of Patent: March 3, 2015
    Assignee: Photonic Biosystems Inc.
    Inventors: David L. Putnam, Jason A. Putnam, Todd W. Hubbard
  • Patent number: 8951765
    Abstract: Embodiments described herein relate to separating and/or concentrating target cells from a carrier fluid that may include other non-target cells. Embodiments include a cell separator with a flow surface having indentations formed thereon. The indentations are configured to capture target cells by physical and/or chemical interactions. The indentations may also include a layer of support molecules that assist in releasing captured cells for collection.
    Type: Grant
    Filed: November 24, 2009
    Date of Patent: February 10, 2015
    Assignee: Empire Technology Development LLC
    Inventor: Masahiro Ueda
  • Patent number: 8951788
    Abstract: The invention relates to a filtration unit, comprising a membrane filter that can be disposed on a filter support of a bottom part and an attachment that can be placed on the bottom part, wherein the membrane filter has a reinforcing edge, and wherein the membrane filter can be clamped to a clamping part of a cover of a culture medium unit for removal purposes and introduced into the culture media unit. The invention further relates to a method for the microbiological analysis of liquid samples following a filtration process, wherein after removing an attachment a membrane filter is lifted off a filter support for pouring in the liquid sample and set down on a surface of a culture medium disposed in the bottom part of a culture medium unit, and the bottom part is covered by a cover. The cover is placed on the membrane resting on the filter support such that a clamping part present on the filter support clamps to a reinforcing edge of the membrane.
    Type: Grant
    Filed: February 16, 2008
    Date of Patent: February 10, 2015
    Assignee: Sartorius Stedim Biotech GmbH
    Inventor: Karl Pflanz
  • Publication number: 20150024373
    Abstract: A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.
    Type: Application
    Filed: July 16, 2013
    Publication date: January 22, 2015
    Inventors: Zheng XIA, Yu ZHOU, John LARSEN, Guocheng SHAO, Shane PETERSON, Marjorie FAUST
  • Patent number: 8932858
    Abstract: Hydrophobically modified polysaccharide compositions, articles incorporating the compositions, and methods for cell culture, automatic release of cells, and release of cell culture coating from a substrate or the cell culture articles incorporating the coating compositions, as defined herein.
    Type: Grant
    Filed: March 4, 2009
    Date of Patent: January 13, 2015
    Assignee: Corning Incorporated
    Inventors: Wendy A. Baker, Dana C. Bookbinder, Theresa Chang, Robert R. Hancock, Jr.
  • Patent number: 8927243
    Abstract: The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: January 6, 2015
    Assignee: University of South Florida
    Inventor: Matt Ewert
  • Patent number: 8921096
    Abstract: The invention concerns a pump cart for a biological liquid treatment installation, having a first lateral face (40), and a second lateral face (41) by which it is configured to be juxtaposed against a conveying network cart (1) of the installation and a front face (42) meeting the lateral faces (40, 41), and comprising at least one pump (414), a pump support (50) on which is mounted said pump (414), and a guide member for making said pump (414) movable in translation and on which said support (50) is mounted; said support (50) being movable in translation in a direction going from the first lateral face (40) towards the second lateral face (41); whereby said pump (414) is disposed at a predetermined location on said cart (2) depending on the type of treatment carried out.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: December 30, 2014
    Assignee: EMD Millipore Corporation
    Inventors: Jean-Louis Weissenbach, Rene Reinbigler, Virginie Buisson, Christine Abouayad El Idrissi, Sebastien Cirou
  • Publication number: 20140377834
    Abstract: An acoustic standing wave is utilized to separate components from a multi-component fluid, such as animal cells from fluid-cell mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of falling cells as the cells coalesce, agglomerate, and the weight of the agglomerated mass overcomes the drag and ultrasonic standing wave forces in the device.
    Type: Application
    Filed: June 24, 2014
    Publication date: December 25, 2014
    Inventors: Walter M. Presz, Jr., Bart Lipkiens, Jason Dionne, Thomas J. Kennedy, III
  • Patent number: 8906674
    Abstract: A sample preparation apparatus comprising: a storage chamber that can store therein a liquid sample including an analysis target to be analyzed; a concentrated sample storage chamber that is provided to communicate with the storage chamber and that stores therein concentrated liquid having an analysis target having a higher concentration than that of the liquid sample; and an analysis target transportation section for transporting the analysis target included in the liquid sample stored in the storage chamber to the concentrated sample storage chamber. A cell analyzer is also disclosed.
    Type: Grant
    Filed: September 29, 2010
    Date of Patent: December 9, 2014
    Assignee: Sysmex Corporation
    Inventors: Ryuichiro Ebi, Koki Tajima
  • Patent number: 8906229
    Abstract: The invention concerns a device comprising: a base (2); a moveable or removable door (20), said device having a closed door position; and in the closed door position, a circuit (8) comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed on said front face (5) of said base (2) and a second shell (17) disposed in said door (20); said bag being clamped between said first shell (16) and said second shell (17) in a state in which conduits of said network for conveying liquid are formed between said films.
    Type: Grant
    Filed: May 26, 2011
    Date of Patent: December 9, 2014
    Assignee: EMD Millipore Corporation
    Inventors: Sebastien Cirou, Rene Reinbigler, Virginie Buisson, Jean-Louis Weissenbach
  • Patent number: 8900454
    Abstract: The invention concerns a device comprising a base (2) and a door (20), said device having a closed door position in which a circuit (8) of the device comprises a bag comprising two flexible films and connectors of the conveying network, and a press (9) comprising a first shell (16) disposed on a front face (5) of said base (2) and a second shell (17) disposed in said door (20); and a hinge system hinging said door (20) relative to said base (2), and disposed only on one side of said door (20) so as to form lateral clearances between said door (20) and said base (2) over the rest of a perimeter of said door (20).
    Type: Grant
    Filed: June 6, 2011
    Date of Patent: December 2, 2014
    Assignee: EMD Millipore Corporation
    Inventors: Sebastien Cirou, Rene Reinbigler, Virginie Buisson, Jean-Louis Weissenbach
  • Patent number: 8895279
    Abstract: A method to recover and harvest nutrients from a liquid stream by incorporating them into microorganisms grown in a rotating photobioreactor. The method further includes optionally integrating the rotating photobioreactor with a composting or biogenic drying process.
    Type: Grant
    Filed: September 3, 2013
    Date of Patent: November 25, 2014
    Inventor: Dennis A. Burke
  • Patent number: 8889388
    Abstract: The invention provides a settling device comprising an acoustic wave generator and an inclined settling chamber. The angle ? between the acoustic wave direction and the inclined settling chamber is greater than 0 and less than 90°. The invention also provides a concentration method and a separation method using the device. The invention can be used to concentrate or separate particles such as inorganic particles, organic particles, and biological particles, for example, mammalian cells, bacteria, yeast, algae, and plant cells. The invention exhibits technical merits such as higher efficiency, cost-effectiveness, and large-scale production.
    Type: Grant
    Filed: April 21, 2011
    Date of Patent: November 18, 2014
    Inventors: Zhaowei Wang, Donald Feke, Joanne Belovich
  • Publication number: 20140322745
    Abstract: The present invention relates to a method of sampling biological material on a reference surface, such as the surface of an agar medium, with the aid of a sampling tool, said method making it possible to steer the displacement of the sampling tool towards the reference surface, from a first position towards a second position, and/or of the reference surface towards the sampling tool, from a first position towards a second position, said second position being a contact position in which the sampling tool and the reference surface are in contact, said method comprising the following steps: a) illuminating the sampling tool in said first position in order to make the sampling tool visible and to project an image of the sampling tool onto the reference surface, b) acquiring an image of both the sampling tool and its image projected onto the reference surface, c) processing the image obtained in step b) in order to determine if, on the processed image, the gap between the sampling tool and its projected image is
    Type: Application
    Filed: November 16, 2012
    Publication date: October 30, 2014
    Inventors: Dominique Decaux, Régis Montvernay, Frédéric Pinston
  • Patent number: 8852435
    Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.
    Type: Grant
    Filed: November 29, 2011
    Date of Patent: October 7, 2014
    Assignee: Therapeutics Proteins International, LLC
    Inventor: Sarfaraz Niazi
  • Patent number: 8846384
    Abstract: The present invention relates to a system for detecting microbial contamination of a liquid specimen comprising a device for concentrating micro-organisms from a liquid specimen, having (i) a hyperbaric chamber, (ii) a filter housing comprising a liquid-permeable bed of an adsorbent material and adapted for being fluidly connected to the hyperbaric chamber, and (iii) a manner for pressurizing the hyperbaric chamber. The system also comprises a kit for detection of micro-organisms adsorbed to the adsorbent material, wherein the kit is based on enzymatic detection using chromogenic and/or fluorescent substrate analogues.
    Type: Grant
    Filed: November 24, 2008
    Date of Patent: September 30, 2014
    Assignee: Nederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek TNO
    Inventors: Jan Gerritse, Johannes Wouterus van Groenestijn, Neeltje Dina Zegers
  • Publication number: 20140273207
    Abstract: Methods are disclosed for isolation of a stem-cell derived differentiated cell, which method entails illuminating an incident light onto a plurality of stem-cell derived cells that comprise at least an undifferentiated cell and a differentiated cell possessing a noncentrosymmetric structure, wherein the differentiated cell generates second-harmonic light from the incident light; and isolating the differentiated cell identified by the second-harmonic light. Devices for carrying out the methods are also provided.
    Type: Application
    Filed: October 26, 2012
    Publication date: September 18, 2014
    Inventors: James W. Chan, Deborah Lieu
  • Publication number: 20140271909
    Abstract: The disclosure relates to an apparatus for segregating particles on the basis of their ability to flow through a stepped passageway. At least some of the particles are unable to pass through a narrower passageway bounded by a segregating step, resulting in segregation of the particles. The breadth of the leading edge of at least one step of the apparatus is significantly greater than the overall width of the passageway in which the step occurs, permitting high and rapid sample throughput. The apparatus and methods described herein can be used to segregate particles of a wide variety of types. By way of example, they can be used to segregate circulating tumor cells from a human blood sample.
    Type: Application
    Filed: November 12, 2013
    Publication date: September 18, 2014
    Inventor: George HVICHIA
  • Publication number: 20140273176
    Abstract: Systems and methods for processing biological material utilizing chitosan are provided herein. Methods may include the steps of reducing the pH of an amount of culture to about 5 to 7, incorporating an amount of a chitosan solution into the culture to form a mixture, wherein chitosan in the chitosan solution binds to the biological material; increasing the pH of the culture to about 7 to 10, to flocculate biological material in the culture, concentrating the flocculates, reducing the pH of the concentrated flocculates to about 3 to 5, and separating the recovered chitosan solution from the concentrated biological material.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Inventor: Daniel Fleischer
  • Publication number: 20140273056
    Abstract: A device and a method for isolating a target from a sample are provided. The target is bound to solid phase substrate to form a target bound solid phase substrate. The device includes a first plate having a first region for receiving at least a portion of the sample. A second plate is spaced from the first plate by a distance and has a first region for receiving a reagent. A force attracts the target bound solid phase substrate toward the first region of the second plate such that the target bound solid phase substrate in the portion of the sample are drawn through the air gap and into the reagent by the force.
    Type: Application
    Filed: March 14, 2013
    Publication date: September 18, 2014
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: David J. Beebe, Scott M. Berry
  • Publication number: 20140248656
    Abstract: A method for sorting motile cells includes introducing an initial population of motile cells into an inlet port of a microfluidic channel, the initial population of motile cells having a first average motility; incubating the population of motile cells in the microfluidic channel; and collecting a sorted population of motile cells at an outlet port of the microfluidic channel. The sorted population of motile cells has a second average motility higher than the first average motility.
    Type: Application
    Filed: May 18, 2012
    Publication date: September 4, 2014
    Applicant: THE BRIGHAM AND WOMEN S HOSPITAL, INC.
    Inventors: Utkan Demirci, Xiaohui Zhang, Emre Kayaalp, Hooman Safaee, Savas Tasoglu
  • Patent number: 8822211
    Abstract: The invention concerns a device and a method for concentrating pathogenic germs potentially present in blood products or derivatives and for detecting said germs comprising the following steps: (a) subjecting a sample of said blood product to a blood cell aggregating treatment, (b) eliminating the aggregates formed at step (a) by passing the treated sample over a first filter allowing through the contaminating germs but not the cell aggregates, (c) selectively lyzing the residual cells of the filtrate obtained at step (b), (d) recuperating the contaminating germs by passing the lysate of step (c) over a second filter to detect the contaminating germs possibly trapped.
    Type: Grant
    Filed: June 11, 2013
    Date of Patent: September 2, 2014
    Assignee: Becton Dickinson Infusion Therapy Systems Inc.
    Inventors: Jean-Pierre Hermet, Isabelle Besson-Faure, Sebastien Ribault, Yann Godfrin, Anne Monnot des Angles
  • Patent number: 8802391
    Abstract: The present invention is directed to a method for measuring the migration of cells in a channel under the influence of an analyte wherein said cells are separated from said analyte by a semi-permeable membrane and said cells are subjected to controlled flow conditions while the analyte is static and in the form of a viscous substance or gel.
    Type: Grant
    Filed: October 10, 2012
    Date of Patent: August 12, 2014
    Assignee: Cellix Limited
    Inventors: Dmitry Kashanin, Igor Shvets, Vivienne Williams, Frank O'Dowd
  • Publication number: 20140220672
    Abstract: Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates, adjustable height cap for same, as well similar devices for multi-magnet culture plates. A pen-like device for sterilely lifting and moving cells is also described, and this magnetic pipettor can also exist in multi-well magnetic pipettor formats.
    Type: Application
    Filed: February 7, 2013
    Publication date: August 7, 2014
    Applicant: NANO3D BIOSCIENCES, INC
    Inventor: Nano3D Biosciences, Inc
  • Patent number: 8795530
    Abstract: The present invention relates to methods and systems for optimization of dilution of a viscous starting material to isolate and/or concentrate the product of interest from the starting source material such that the process minimizes the volume of diluent and the total volume of the waste stream generated during the process as well as maximizing the yield of desired product. The system employs cross-flow filtration modules with sub-channels that are equidistant to the inlet and outlet of said modules and such modules are characterized by optimal channel height, optimal transmembrane pressure, etc., which are selected in order to achieve the best combination of product quality and production yield.
    Type: Grant
    Filed: January 21, 2010
    Date of Patent: August 5, 2014
    Assignee: Smartflow Technologies, Inc.
    Inventors: Henry B. Kopf, James A. Kacmar
  • Patent number: 8778670
    Abstract: A method and apparatus for embedding cells that utilizes a flow-through embedding technique. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. The apparatus produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick and efficient manner.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: July 15, 2014
    Assignee: University of Massachusetts
    Inventor: Andrew H. Fischer
  • Publication number: 20140193900
    Abstract: A device and method are disclosed to enable fluid manipulations without disturbing or removing particles or cells. By engineering assay compartment geometries to take advantage of fluid mechanical effects, the flow velocity may be diminished around particles or cells. This technology may be used to process particles or cells of various kinds that are suspended in liquids or gels, and is suitable for use in high throughput screening.
    Type: Application
    Filed: May 29, 2012
    Publication date: July 10, 2014
    Inventors: Ivar Meyvantsson, Steve Hayes, Victoria Echeverria, Robert Lowery, Casey Lamers
  • Publication number: 20140193852
    Abstract: Devices and methods are described for pre-processing tissue samples containing cells of interest for various therapeutic procedures. Fibrous material may be extracted from the tissue sample through a shredding and separating device, resulting in a non-fibrous sample containing the cells of interest, which may then be washed and centrifuged to remove fluids, oil, and contaminants. The sample may then be digested in a large-volume container, and a piston within the container may push the resulting liberated cell suspension through a cell concentrator such as a hollow-fiber separation module.
    Type: Application
    Filed: May 7, 2009
    Publication date: July 10, 2014
    Inventors: Erik Vossman, Shannon Iwami, Anthony Yang, Tom Cannon, Hyun Joon Paek
  • Patent number: 8772020
    Abstract: A fermentation system for production of biogas from solid organic material, the system including at least one fermentation reactor. The fermentation reactor includes a vessel (37) for housing a feed mixture having liquid and solid particles and an outlet port (44), a strainer assembly (33) at the outlet port for filtering processed feed mixture from the vessel, and a biogasification reactor (37) in fluid communication with another fermentation reactor, the other fermentation reactor configured to produce a biomethane. The strainer assembly further includes a mixture driving member (53) positioned in the strainer body (47) adjacent the drain opening (51), the mixture driving member having a driving surface configured to receive the liquid passing through the filter member (60). The driving surface engages the filter member such that solid particles are driven from a surface of the filter member when the driving member is activated.
    Type: Grant
    Filed: August 1, 2007
    Date of Patent: July 8, 2014
    Assignee: The Regents of the University of California
    Inventors: Dave Konwinski, Ruihong Zhang
  • Publication number: 20140186819
    Abstract: A rapid one-pass liquid filtration system efficiently concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. Additional concentration stages may be added in “cascade” fashion, in order to concentrate particles below the size cut of each preceding stage remaining in the separated fluid in a concentrated sample suspension. This process can also be used to create a “band-pass” concentration for concentration of a particular target size particle within a narrow range.
    Type: Application
    Filed: February 7, 2012
    Publication date: July 3, 2014
    Inventors: David S. Alburty, Andrew E. Page, Zachary A. Packingham, Daniel B. Marske
  • Patent number: 8765456
    Abstract: The invention relates to microscale cell separating apparatus which are able to separate cells on the basis of size of the cells, interaction of the cells with surfaces of the apparatus, or both. The apparatus comprises a stepped or sloped separation element (16) interposed between an inlet region (20) and an outlet region (22) of a void that can be tilled with fluid. The void can be enclosed within a cover (12) and fluid flow through the void engages cells with the separation element. Only cells which have (or can deform to have) a characteristic dimension smaller than or equal to the distance between a step and the cover or body can pass onto or past a step. Modifications of surfaces within the apparatus can also inhibit passage of cells onto or past a step.
    Type: Grant
    Filed: June 28, 2011
    Date of Patent: July 1, 2014
    Assignee: Parsortix, Inc.
    Inventor: Georgi Hvichia
  • Publication number: 20140178992
    Abstract: A membrane separation culture device includes an upper structure including a vessel in which at least a portion of the bottom thereof is formed with a separation membrane having pores that allow stem cells to permeate therethrough, and a lower structure including a vessel that retains a fluid in which the membrane of the upper structure is immersed.
    Type: Application
    Filed: March 30, 2012
    Publication date: June 26, 2014
    Applicant: National Center for Geriatrics and Gerontology
    Inventors: Misako Nakashima, Koichiro Iohara, Kazumasa Yamada, Masaaki Shimagaki, Masahiro Osabe
  • Publication number: 20140154687
    Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included, for collecting the microbial cells adjacent to a liquid interface formed by the cushioning liquid, upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed to prior to extraction of the pretreated sample.
    Type: Application
    Filed: March 15, 2013
    Publication date: June 5, 2014
    Inventors: Samad TALEBPOUR, Aye Aye KHINE, Robert MAASKANT, Tino ALAVIE
  • Publication number: 20140154664
    Abstract: A micro fluidic apparatus includes a sample chamber that divides a sample into a first fluid layer and a second fluid layer due to a centrifugal force; a first enriching unit a that receives the first fluid layer from the sample chamber, forms a first complex of a first fine particle and the first target material, and separates the first complex from the first fluid layer using a density difference; and a second enriching unit that receives the second fluid layer from the sample chamber, forms a second complex of a second fine particle and the second target material, and separates the second complex from the second fluid layer using a density difference.
    Type: Application
    Filed: July 12, 2013
    Publication date: June 5, 2014
    Inventors: Min Seok S. KIM, Jong-myeon PARK
  • Publication number: 20140134646
    Abstract: This invention provides methods and compositions for capturing circulating tumor cells (CTCs) as well as various divergent CTC phenotypes using seprase-specific affinity reagents. Methods of analyzing CTCs and assessing their metastatic potential in vivo and in vitro are also disclosed.
    Type: Application
    Filed: November 8, 2013
    Publication date: May 15, 2014
    Applicants: ROCHE MOLECULAR SYSTEMS, INC., THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL, BIOFLUIDICA, INC
    Inventors: George Martin, Steven Soper, Makgorzata Witek, Jen Jen Yeh
  • Patent number: 8703478
    Abstract: An apparatus and method for holding a membrane, screen or other flexible planar body in tension, while providing a conduit for water or other liquid to flow to the membrane being held. The membrane extends from inside a manifold body that carries the liquid, and the manifold body supports the membrane at one edge while the membrane is pulled in tension. Liquid pressure builds up inside the manifold body, preferably by entering a pressure chamber at the top of the manifold body. At a feeding pressure in the pressure chamber the liquid is distributed to the membrane for microbe growth. The liquid can be elevated to a higher, microbe-harvesting pressure by increasing the pressure in the pressure chamber, thereby deflecting a shim separating the pressure chamber from the membrane. The change in pressure is carried out by manually or automatically opening and closing a conventional water valve.
    Type: Grant
    Filed: May 19, 2008
    Date of Patent: April 22, 2014
    Assignee: Ohio University
    Inventors: David J. Bayless, Morgan LeFay Vis-Chiasson, Gregory G. Kremer, Ben Stuart
  • Patent number: 8703457
    Abstract: A cell separation apparatus comprises a means for moving a cell within a cell separating space by applying an electrical voltage to cells in the cell separating space using gel electrodes, and channels for separating and discharging the cell, which thus does not damage a cell sample and prevents the exhaustion of an electrode due to its electrolysis.
    Type: Grant
    Filed: April 30, 2004
    Date of Patent: April 22, 2014
    Assignee: On-Chip Cellomics Consortium Co., Ltd.
    Inventors: Kenji Yasuda, Kazunori Takahashi
  • Publication number: 20140106388
    Abstract: An embodiment relates to a method and to an array for detecting live circulating or disseminated cells in body fluids (for example blood, urine) or tissue samples (for example bone marrow) mixed with liquid. The method includes: a) filtering the liquid sample through a porous membrane that is suitable for retaining cells to be detected, such that cells to be detected come to rest upon at least a part of the surface of the membrane and the sample liquid passes the membrane; b) applying a first process liquid containing a first agent for marking the cells to be detected with a first marker; c) incubating the process liquid on the membrane for a predetermined time period, wherein the cells to be detected are marked; and d) detecting the marked cells to be detected on the surface of the membrane.
    Type: Application
    Filed: April 13, 2012
    Publication date: April 17, 2014
    Applicant: SIEMENS AKTIENGESELLSCHAFT
    Inventors: Joachim Bangert, Katja Friedrich, Walter Gumbrecht, Karsten Hiltawsky, Peter Paulicka, Manfred Stanzel