Determining Presence Or Kind Of Micro-organism; Use Of Selective Media Patents (Class 435/34)
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Patent number: 10494638Abstract: The present disclosure provides methods and kits for generating recombinant bacteriophage genomes. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage DNA genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.Type: GrantFiled: February 9, 2018Date of Patent: December 3, 2019Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Jason Holder, Connor McBrine, Miles Rogers, Nicole Billings, Sarah Gruska, Lucas Tilley
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Patent number: 10472662Abstract: Presented herein are solid supports on or in which a composition comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent) is present in a dried form. Presented herein are also methods for detecting the presence of an intracellular microorganism target (e.g., an intracellular bacterial target) utilizing such solid supports. Further, presented herein are tablets as well as dry powders comprising a lysis reagent and an intracellular microorganism target detection reagent (e.g., an intracellular bacterial target detection reagent), and methods of using such tablets and dry powders to detect the presence of an intracellular microorganism target (e.g., an intracellular bacterial target).Type: GrantFiled: December 1, 2017Date of Patent: November 12, 2019Assignee: Becton, Dickinson and CompanyInventors: Mei Yang-Woytowitz, Charles Yu, Timothy Wiles
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Patent number: 10475527Abstract: A method for determining whether microbial growth is occurring within a specimen container includes steps of incubating the specimen container and obtaining a series of measurement data points while the specimen container is incubated and storing the data points in a machine-readable memory. The series of measurement data points represent a growth curve of microbial growth within the specimen container. The methods determine a positive condition of microbial growth within the container from the measurement data points.Type: GrantFiled: March 7, 2013Date of Patent: November 12, 2019Assignee: bioMerieux, Inc.Inventor: Michael Ullery
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Patent number: 10465231Abstract: A method and an apparatus are provided for enriching pathogen DNA. The method includes adding a selective lysis buffer to a sample, incubating the mixture formed thereby, and filtering the mixture. The apparatus for enriching pathogen DNA includes: a lysis chamber; a reservoir containing selective lysis buffer, connected to the lysis chamber; and a filter connected to the lysis chamber; that achieves a limit of detection of pathogens of 500 cfu/ml or less.Type: GrantFiled: March 15, 2017Date of Patent: November 5, 2019Assignee: Siemens Healthcare GmbHInventors: Divya Khandige Sharma, Ragavendar MS, Nivedita Mitra, Ramya Vutukuru, Yiwei Huang
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Patent number: 10457908Abstract: The present invention relates to a method of analysis comprising the preparation and the analysis of a sample in a flexible container without direct handling of the sample and without reopening the flexible container at the end of the preparation of the sample, and also to a container and a kit that enable the implementation of this method.Type: GrantFiled: April 3, 2015Date of Patent: October 29, 2019Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE-CNRS, PRESTODIAG, COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVESInventors: Roberto Calemczuk, David Carrara, Thierry Livache, Thibaut Mercey, Félix Piat, Yoann Roupioz, Sami Slimani
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Patent number: 10451638Abstract: Disclosed is a blood analyzer that includes: a sample preparation unit configured to mix a blood specimen and a fluorescent dye for staining nucleic acid to prepare a measurement sample; a light receiver configured to receive fluorescence and scattered light that are generated by light being applied to the measurement sample prepared by the sample preparation unit; and a controller programmed to determine whether or not infection with Plasmodium has occurred, on the basis of a value representing variation of a distribution of first particles in a range where single-ring form of red blood cells appear, the range where single-ring form of red blood cells appear being identified according to fluorescence intensities and scattered light intensities obtained by processing of signals from the light receiver.Type: GrantFiled: July 24, 2017Date of Patent: October 22, 2019Assignee: SYSMEX CORPORATIONInventors: Yuhgi Suzuki, Takuma Watanabe
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Patent number: 10443084Abstract: Conjugates of 2,3-dihydroxynaphthalene and its derivatives with enzyme cleavable groups are chromogenic substrates that form colored compounds when complexed with metal ions, e.g. iron ions, on cleavage by enzymes, and are useful in microbial detection and identification. The cleavage products form purple or red-brown colored complexes, that can easily be observed by the naked eye. Microbes can be grown in the presence of the substrates and the metal salts that provide the metal ion for complexing with the 2,3-dihydroxynaphthalene product. Substituents in the naphthalene ring may affect the solubility of the substrates and also the diffusibility and color of the metal complexes. Some of the substrates yield soluble complexes on cleavage and are of particular value in liquid growth media. Other substrates produce less soluble complexes that are more suitable for use in solid agar media.Type: GrantFiled: February 27, 2018Date of Patent: October 15, 2019Assignee: GLYCOSYNTH LIMITEDInventors: Hayley Jane Turner, Michael Burton
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Patent number: 10413592Abstract: The present invention relates to improved assisted reproductive technology using highly purified menotropin (HP-hMG) to stimulate follicle development in controlled ovarian stimulation, particularly in women at risk of a high ovarian response to controlled ovarian stimulation.Type: GrantFiled: February 25, 2016Date of Patent: September 17, 2019Assignee: FERRING B.V.Inventors: Joan-Carles Arce, Jane Ruman
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Patent number: 10415075Abstract: A method is described to separate, characterize and/or identify microorganisms in a test sample. Said method comprises the steps of (i) lysing non-microorganism cells that might be present in the sample, (ii) separating the microorganisms as a pellet in the sample post-lysing non-microorganism cells, (iii) interrogating the pellet containing microorganisms by mass spectrometry to produce mass spectra of microorganisms, and (iv) characterizing and/or identifying microorganisms in the sample. The method may be used in characterizing/identifying microorganisms in complex samples, e.g., blood containing culture media.Type: GrantFiled: October 30, 2009Date of Patent: September 17, 2019Assignee: bioMerieux, Inc.Inventors: Jones Hyman, Bradford Clay, John Walsh, Thurman Thorpe
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Patent number: 10415081Abstract: The invention provides methods and processes for the identification of polymorphisms at one ore more designated sites, without interference from non-designated sites located within proximity of such designated sites. Probes are provided capable of interrogation of such designated sites in order to determine the composition of each such designated site. By the methods of this invention, one ore more mutations within the CFTR gene and the HLA gene complex can be identified.Type: GrantFiled: August 4, 2016Date of Patent: September 17, 2019Assignee: BioArray Solutions Ltd.Inventors: Alice Xiang Li, Ghazala Hashmi, Michael Seul
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Patent number: 10406227Abstract: Methods and compositions modulate the activity of electrically excitable cells. Photovoltaic compounds which, upon exposure to light energy, increase or decrease the electrical activity of cells. These supplement and/or replace of vision based on the conversion of light energy to electrical energy within certain cells of the visual system. A “patch” or bridge to circumvent one or more defective, damaged, or diseased cells in the visual system. Additionally, in several embodiments, subjects with normal vision can benefit from the methods, compositions, systems, and/or devices disclosed herein as normal visual acuity can be heightened. The exposure induces an energy (e.g., a receipt of light energy, conversion to electrical energy, and passage of that electrical energy) from the photovoltaic compound to the cell, thereby altering the transmembrane potential of the cell and/or the opening of one or more ion channels, thereby modulating the activity of the electrically excitable cell.Type: GrantFiled: December 18, 2013Date of Patent: September 10, 2019Assignees: California Institute of Technology, University of Southern CaliforniaInventors: Robert H. Chow, Mark S. Humayun, Harry B. Gray, Robert H. Grubbs, Dennis A. Dougherty, Mark E. Thompson, Lionel E. Cheruzel, Melanie A. Yen
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Patent number: 10408735Abstract: An in vitro method for the marker-free determination of a cell type of a cell in a biological sample is disclosed, a microscopic device being configured to detect a height profile of the cell with respect to a carrier plate. An embodiment of the method performed by a cell analysis device, include: determining a cell compartment of the cell on the basis of the detected height profile, determining a predetermined quantitative cell feature on the basis of the determined cell compartment, and determining the cell type of the cell on the basis of the determined quantitative cell feature. A correspondingly designed cell analysis device and a corresponding microscopic device are also disclosed.Type: GrantFiled: September 30, 2014Date of Patent: September 10, 2019Assignee: Siemens Healthcare GmbHInventors: Oliver Hayden, Oliver Schmidt
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Patent number: 10392648Abstract: Provided are an airborne microbial measurement apparatus and a method of measuring the same. The airborne microbial measurement apparatus includes a particle separation device including a main body having a flow space in which airborne microorganism flows and a collection unit separably coupled to one side of the main body to collect the airborne microorganism, a reagent container in which a lysis reagent reacting with the airborne microorganism collected in the collection unit and a luminous material are stored, and a luminescence measurement device for measuring intensity of light emitted after the airborne microorganism reacts with the lysis reagent and the luminous material.Type: GrantFiled: December 16, 2014Date of Patent: August 27, 2019Assignees: LG ELECTRONICS INC., INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITYInventors: Chulwoo Park, Yeekyeong Jung, Sunghwa Lee, Jungho Hwang, Jiwoon Park
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Patent number: 10386376Abstract: A test device for detection and visual identification of a specific analyte in a liquid sample such as a bodily fluid is disclosed. The device includes a collection container, a test strip affixed to an interior surface of the collection container, and a removable protective strip adhered over the test strip. The protective strip is configured to be removed from contact with the test strip after the liquid sample has been collected in order to prevent unnecessary exposure and/or contamination of the test strip. In certain embodiments, the protective strip may be removed from the contact with the test strip when the collection container is in a sealed configuration. In other embodiments, the protective strip may be dissolvable when placed in contact with the desired liquid sample. An assay specific to the anticipated analyte may be provided on the test strip.Type: GrantFiled: November 9, 2015Date of Patent: August 20, 2019Assignee: JEIMEI, LLCInventor: Christina Holloway
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Patent number: 10380807Abstract: A pipette check station for checking the calibration or service status of a pipette includes an RFID reader, a user interface with a display and buttons, and a digital interface to connect the check station to additional equipment; the check station optionally further includes provisions to hold pipettes for storage and to charge electronic pipettes held thereupon.Type: GrantFiled: January 1, 2018Date of Patent: August 13, 2019Assignee: Mettler-Toledo Rainin, LLCInventors: Gerhard Keller, Richard Hill, Deryl Stanley, James Petrek, Murray Anderson
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Patent number: 10351896Abstract: Use of at least one chromogenic and/or fluorogenic carboxylesterase and/or triacylglycerol-lipase substrate, to detect bacteria of the Bacillus cereus group in a sample capable of containing them.Type: GrantFiled: December 23, 2014Date of Patent: July 16, 2019Assignee: BIOMERIEUXInventors: Marie Cellier, Marie-Pierre Bourguignon, Diane Halimi
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Preloaded test substrates for testing LAL-reactive substances, methods of use, and methods of making
Patent number: 10352934Abstract: A test substrate for detecting a LAL-reactive substance, wherein at least a portion of said test substrate has been preloaded with at least one LAL reagent and/or at least one LAL-reactive standard. Methods of use of the test substrate are disclosed. Methods of depositing test reagents on a test substrate are also disclosed.Type: GrantFiled: September 28, 2017Date of Patent: July 16, 2019Assignee: GENERAL ELECTRIC COMPANYInventors: Richard Douglas Godec, Paul Charles Melanson, Matthew Kaddeland Stonesmith, Hong Xu, Yan Huang -
Patent number: 10345259Abstract: The present invention provides a method for the quantification of virus particles in a biological sample, comprising the steps of: (a) introducing said biological sample comprising virus particles into a capillary tube containing a buffer solution; (b) applying an electrical field to said capillary tube of sufficient voltage to allow for the separation of the virus particles from additional constituents in said sample, to obtain electrophoretical fractions; (c) generating an electropherogram associated with the electrophoretical fractions; and (d) determining the concentration of virus particles in said sample by comparing the electropherogram with an electropherogram generated from a reference sample containing a known concentration of said virus particles.Type: GrantFiled: February 18, 2016Date of Patent: July 9, 2019Assignee: Janssen Vaccines & Prevention B.V.Inventors: Ewoud Van Tricht, Lars Johannes Geurink, Catharina Elise Sänger-Van De Griend
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Patent number: 10330584Abstract: A cell analyzer and a particle sorting method and device are disclosed. The method comprises: acquiring a pulse width of at least one optical signal according to a detected optical signal, selecting at least one optical signal as a combined optical signal, and respectively calculating a signal intensity of the combined optical signal with the pulse width in a combinatorial way to obtain at least one reinforcement signal, where a difference between a first category of particles and a second categories of particles in the reinforcement signal is increased relative to a difference therebetween in the combined optical signal; and on the basis of the reinforcement signal and at least another signal, forming a new scatter diagram, where the at least another signal is one of other reinforcement signals and the optical signal, distinguishing the first category of particles from the second category of particles according to the new scatter diagram.Type: GrantFiled: August 11, 2017Date of Patent: June 25, 2019Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.Inventors: Bo Ye, Guanzhen Wang, Jiantao Di, Ying Zhang, Huan Qi
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Patent number: 10311573Abstract: Systems, methods, and devices for classifying or detecting mold samples or training computer models (such as neural networks), are disclosed. A method includes obtaining a microscopy image of a mold sample. The method includes determining a classification of the mold sample based on non-image data corresponding to the mold sample. The method further includes training a computer model based on the microscopy image and a label indicating the classification.Type: GrantFiled: May 2, 2017Date of Patent: June 4, 2019Assignee: Techcyte, Inc.Inventors: S. Russell Zimmerman, Ralph Yarro, III, Benjamin P. Cahoon, Richard Boyd Smith, Hyrum S. Anderson
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Patent number: 10288569Abstract: A method for preparing a hydrogel that reversibly changes color depending on pH includes: (S10) dissolving a carboxyl group-containing polysaccharide in buffer to obtain a polysaccharide solution; (S20) adding a cross-linker solution composed of a mixture of an organic solvent and a cross-linker to the polysaccharide solution; (S30) adding, to the cross-linker-containing polysaccharide solution resulting from step (S20), an organic solution composed of a mixture of an organic solvent and a first organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto, to form a mixture solution, and allowing the mixture solution to react; (S40) obtaining a polymer complex from the reaction mixture resulting from step (S30); and (S50) mixing the polymer complex from step (S40) with an organic dye comprising a second organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto.Type: GrantFiled: February 8, 2017Date of Patent: May 14, 2019Assignee: INDUSTRY-ACADEMIC CORPORATION FOUNDATION YONSEI UNIVERSITYInventors: Sang Yup Lee, Chaemyeong Lee, Min Chul Kim, Jin Young Kwak, Sang Woo Park, Young Jun Lim
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Patent number: 10280446Abstract: The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms.Type: GrantFiled: July 17, 2017Date of Patent: May 7, 2019Assignee: 3M Innovative Properties CompanyInventors: Patrick A. Mach, Michelle L. Rosauer, Michael E. Hughes
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Patent number: 10260082Abstract: Liposomal detection devices and methods of making and using such devices are disclosed. Such liposomal detection devices may be used to detect microbes by detecting a byproduct of microbial metabolism, or may be used to detect changes in pH and/or changes in temperature. Liposomal detection devices may include a first liposome encapsulating a first destabilizing compound and a second liposome encapsulating a second destabilizing compound. The first liposome may destabilize in response to the detection of the target compound or change to release the first destabilizing compound which may destabilize the second liposome. A matrix, such as a hydrogel matrix, may encase the first liposome and the second liposome.Type: GrantFiled: October 22, 2013Date of Patent: April 16, 2019Assignee: EMPIRE TECHNOLOGY DEVELOPMENT LLCInventors: Michael Keoni Manion, George Charles Peppou
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Patent number: 10233476Abstract: An apparatus (1) for analyzing a contaminated surface has a transfer device (2) with a mount (3) and a porous disk-shaped medium (5) having a contact side (6) arrangeable on the contaminated surface. The mount (3) is bindable to the medium on its side (8) facing away from the contact side (6) via a first fixing edge (7a). An analytical device (11) is bindable to the medium (5) on its contact side (6) via a second fixing edge (7b) to remove the medium (5) from the mount (3). The first fixing edge (7a) is bindable to the medium (5) via a first adhesive bond and the second fixing edge (7b) is bindable to the medium (5) via a second adhesive bond. The first adhesive bond is breakable by a lower application of force than the second adhesive bond.Type: GrantFiled: June 8, 2011Date of Patent: March 19, 2019Assignee: SARTORIUS STEDIM BIOTECH GMBHInventor: Karl Pflanz
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Patent number: 10227629Abstract: Methods for diagnosing, treating, and monitoring the treatment of invasive aspergillosis (IA) are described. The methods can include detecting the presence of one or more volatile organic compounds (VOCs) in the breath of subjects suspected of having IA.Type: GrantFiled: June 4, 2015Date of Patent: March 12, 2019Assignee: The Brigham and Women's Hospital, Inc.Inventors: Sophia Koo, Horatio R. Thomas, Lindsey R. Baden, Francisco M. Marty
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Patent number: 10228312Abstract: The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.Type: GrantFiled: February 25, 2013Date of Patent: March 12, 2019Assignee: Juno Therapeutics GmbHInventor: Herbert Stadler
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Patent number: 10175238Abstract: The present invention relates to methods of determining the presence or absence of abnormal lymphoid cells or abnormal myeloid cells in a cell sample, with the methods comprising subjecting the cell sample to conditions that will activate any inactive matriptase present in the cell sample and measuring the levels of activated matriptase in the cell sample. Once measured, these levels can then be compared to control levels of active matriptase to determine if the cell sample has elevated levels of activated matriptase over control levels of active matriptase. An elevation in the levels of activated matriptase in the cell sample is indicative that the cell sample contains abnormal lymphoid cells or abnormal myeloid cells.Type: GrantFiled: May 13, 2015Date of Patent: January 8, 2019Assignee: Georgetown UniversityInventors: Chen-Yong Lin, Michael D. Johnson, Feng-Pai Chou
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Method for detection, characterization and/or identification of microorganisms in a sealed container
Patent number: 10167494Abstract: The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.Type: GrantFiled: October 30, 2009Date of Patent: January 1, 2019Assignee: bioMerieux, Inc.Inventors: John Walsh, Jones M. Hyman, Thurman Thorpe, Bradford Clay -
Patent number: 10157734Abstract: Methods and systems for identification of microorganisms either after isolation from a culture or directly from a sample. The methods and systems are configured to identify microorganisms based on the characterization of proteins of the microorganisms via high-resolution/mass accuracy single-stage (MS) or multi-stage (MSn) mass spectrometry. Included herein are also discussion of targeted detection and evaluation of virulence factors, antibiotic resistance markers, antibiotic susceptibility markers, typing, or other characteristics using a method applicable to substantially all microorganisms and high-resolution/mass accuracy single-stage (MS) or multi-stage (MSn) mass spectrometry.Type: GrantFiled: May 22, 2015Date of Patent: December 18, 2018Assignee: OXIOD LIMITEDInventors: James L. Stephenson, Jr., Oksana Gvozdyak, Roger Grist, Clay Campbell, Iain C. Mylchreest, Ian D. Jardine
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Patent number: 10137423Abstract: Disclosed herein are devices and methods for the detection, quantification and/or monitoring of analytes. The systems and methods can be used, for example, to rapidly monitor gases downhole in a well.Type: GrantFiled: July 27, 2015Date of Patent: November 27, 2018Assignee: SCHLUMBERGER TECHNOLOGY CORPORATIONInventors: Kay McGuinness, Nathan Scott Lawrence, Andrew Meredith, Andrew Warrillow, Laurent Pirolli
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Patent number: 10130665Abstract: A method, for the identification of bacterial isolates suitable for use in bacteriotherapy, the method comprising: (i) preparing a suspension of material collected from a host harboring microbiota; (ii) addition of an activator of bacterial spores sufficient to allow growth of bacteria from spores present in the suspension; (iii) culturing the suspension; and (iv) identification of at least one bacterial species within the culture.Type: GrantFiled: May 20, 2013Date of Patent: November 20, 2018Assignee: GENOME RESEARCH LIMITEDInventors: Trevor D. Lawley, Simon Clare, Gordon Dougan
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Patent number: 10126243Abstract: A plankton examination apparatus comprises: a sub-pipe in which a transparent pipe unit; a light source unit which radiates only blue-series light; a detection unit which receives light radiated in the direction on the other side outside the transparent pipe unit through the transparent pipe unit from the light source unit and generates an image of the plankton; and a dichroic mirror installed between the transparent pipe unit and the detection unit in such a way as to transmit only red-series light which belongs to the light radiated by the light source unit and passed through the transparent pipe unit and has a wavelength of 620 nm to 780 nm and to deliver the red-series light to the detection unit.Type: GrantFiled: June 25, 2014Date of Patent: November 13, 2018Assignee: KOREA OCEANIC AND ATMOSPHERIC SYSTEM TECHNOLOGY (KOAST)Inventors: Hyo Hyuc Im, Kyu Young Choi, Deok Whan Choi
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Patent number: 10112976Abstract: The invention relates to a process for solution-phase synthesis of D-Arginyl-2,6-dimethyl-L-tyrosyl-L-lysyl-L-phenylalaninamide, an active ingredient used for both common and rare diseases including a mitochondrial targeted therapy for ischemia reperfusion injury.Type: GrantFiled: June 24, 2015Date of Patent: October 30, 2018Assignee: FLAMMA S.P.A.Inventors: Xinjun Zhao, Minyu Zheng, Xiaozhong Yu, Hanrong Gao, Fabrice Cornille
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Patent number: 10107803Abstract: In one example, a device includes a trench formed in a substrate. The trench includes a first end and a second end that are non-collinear. A first plurality of semiconductor pillars is positioned near the first end of the trench and includes integrated light sources. A second plurality of semiconductor pillars is positioned near the second end of the trench and includes integrated photodetectors.Type: GrantFiled: August 23, 2016Date of Patent: October 23, 2018Assignee: International Business Machines CorporationInventors: Yann Astier, Huan Hu, Ning Li, Devendra K. Sadana, Joshua T. Smith, William T. Spratt
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Patent number: 10093920Abstract: The present invention relates to methods for screening a polypeptide for desired activity against a target molecule In particular, the present invention relates to methods for screening a polypeptide for desired activity against a target molecule by expressing the polypeptide in a bacterial cell and permeabilizing the cell.Type: GrantFiled: December 20, 2010Date of Patent: October 9, 2018Assignee: AFFINITY BIOSCIENCES PTY LTDInventors: Matthew Beasley, Ben Kiefel
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Patent number: 10077464Abstract: A microorganism detection medium, said detection being based on showing the presence of a microbial enzyme activity chosen from esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase activities of microorganisms, preferably said microbial enzyme activity being an esterase activity, said medium comprising: at least one chromogenic and/or fluorogenic substrate specific to the enzyme activity sought, preferably specific to an esterase activity, at least one alkyl(thio)glycoside, at least one solvent (S).Type: GrantFiled: December 24, 2013Date of Patent: September 18, 2018Assignee: BiomerieuxInventors: Marie Cellier, Sylvain Orenga, John Perry
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Patent number: 10059976Abstract: Provided is a microorganism detection method which is highly versatile and applicable to both the test for the detection of specified microorganisms and the microbial enumeration test under the same test conditions for Kampo extract preparations and crude drugs containing a variety of antimicrobial substances, is able to effectively reduce the influence of antimicrobial substances in a test sample, and may detect target microorganisms with high precision. This method is a microorganism detection method in a test sample having antimicrobial activity, including a step of preparing a sample liquid containing a test sample having antimicrobial activity and a step of culturing a target microorganism in a culture medium, the method including allowing a (meth)acrylic acid ester-based synthetic adsorbent to act on the sample liquid and/or the culture medium.Type: GrantFiled: June 10, 2014Date of Patent: August 28, 2018Assignee: TSUMURA & Co.Inventors: Hiroaki Yamamoto, Isao Fukuda
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Patent number: 10041923Abstract: A system for measuring water quality parameters, such as pH, CO2, alkalinity, and so forth, includes sensors or other measuring components integrated onto a circuit board. The circuit board may be contained within a submersible, watertight housing. A spectrophotometer may be located on the circuit board to measure the wavelength and intensity of light transmitted through the water being tested. Sensors for measuring salinity, pressure, temperature, or other properties may also be included on or near the circuit board. A microcontroller for processing these measurements is located on the circuit board. System components used to direct and filter the water, such as certain pumps, filters, and so forth, may be included in the housing off of the circuit board. Integrating the sensors and other measuring components onto a circuit board allows for a compact, low-cost water-quality-measuring system that is capable of making very accurate and precise measurements.Type: GrantFiled: April 12, 2017Date of Patent: August 7, 2018Assignee: SWIFT ENGINEERING, INC.Inventors: Andrew Streett, Marc Rocklinger, Xuewu Liu
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Patent number: 10017808Abstract: A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.Type: GrantFiled: December 5, 2017Date of Patent: July 10, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Nikolay Samusik, Garry P. Nolan, Yury Goltsev
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Patent number: 10006082Abstract: A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.Type: GrantFiled: December 5, 2017Date of Patent: June 26, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Nikolay Samusik, Garry P. Nolan, Yury Goltsev
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Patent number: 10000796Abstract: A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.Type: GrantFiled: December 5, 2017Date of Patent: June 19, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Nikolay Samusik, Garry P. Nolan, Yury Goltsev
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Patent number: 9995738Abstract: The present invention relates to an apparatus and method for detecting and enumerating a rare cell in blood. The apparatus comprises a sample collector configured to collect a blood sample and receive a complex in which a target antibody and a marker are coupled; a measuring kit connected to the sample collector in such a way that a mixture of the blood sample and the complex is injected into the measuring kit, thereby individually trapping a blood cell; and a detector configured to detect and enumerate the rare cell having an antigen-antibody reaction with the target antibody among the blood cell trapped in the measuring kit.Type: GrantFiled: March 13, 2014Date of Patent: June 12, 2018Assignee: Korea University Research and Business FoundationInventors: Sehyun Shin, Chang-Soo Han, Dae Ho Jang
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Patent number: 9977041Abstract: It is determined whether an automatic analysis device is in a state where it is necessary to perform periodic cleaning or periodic replacement on a B/F separation passage of a reaction liquid suction nozzle 120 for B/F separation or the like and a detection passage of a reaction liquid suction nozzle 123 for detection, a detection unit 124, and the like, based on the properties of a specimen, a reagent, and a reaction liquid which is obtained by reacting the specimen and the reagent, an analysis protocol which defines treatment conditions of these solutions, and the number of times of dispensing, feeding, and measuring the solutions; and the determined result is displayed on a display 130 as a signal. Accordingly, the automatic analysis device is provided so as to be able to perform adequate maintenance in accordance with analysis conditions.Type: GrantFiled: December 3, 2013Date of Patent: May 22, 2018Assignee: Hitachi High-Technologies CorporationInventors: Yoshihiro Yamashita, Takaaki Hagiwara, Toshiharu Suzuki
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Patent number: 9976940Abstract: The present invention relates to a composition for DNA staining, which can be safely used for researchers, can prevent DNA photocleavage, and can be applied to various fields. According to the present invention, when DNA is stained by using a fluorescent protein and a particular peptide bound to DNA, such a fluorescent protein-peptide is safe to researchers, suppresses DNA photocleavage, and allows reversible staining, thereby enabling efficient DNA staining. Therefore, the composition for DNA staining of the present invention can be variously used in DNA-related research.Type: GrantFiled: September 9, 2015Date of Patent: May 22, 2018Assignee: INDUSTRY-UNIVERSITY COOPERATION FOUNDATION SOGANG UNIVERSITYInventors: Kyu Bong Jo, Seong Hyun Lee, David C. Schwartz
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Patent number: 9964556Abstract: Microplate reader has measuring space, action source, measuring device for biological structure signals of microplate wells, transport support for positioning wells in relation to an optical axis of the device and controller for the action source, the measuring device and the transport support. An incubation apparatus with a frame accommodates a microplate with wells having bottoms for reducing liquid evaporation. The frame has a first opening surrounded by an inner wall for the microplate and an outer wall parallel to the inner wall and connected thereto by an intermediate bottom so that a channel is formed and the intermediate bottom for accommodating a liquid adjusted to the content of the microplate wells. The incubation apparatus has a support surface with a second opening for a microplate and, as a result, at least a portion of the well bottoms is freely accessible by the second opening.Type: GrantFiled: October 30, 2014Date of Patent: May 8, 2018Assignee: TECAN TRADING AGInventors: Gyoergy Wenczel, Harald Gebetsroither, Friedrich Menges
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Patent number: 9958436Abstract: Methods for determining cell chirality using micropatterned substrates are disclosed. Also provided are methods for diagnosing diseases such as genetic diseases or cancer by comparing the chirality of sample cells from a subject with normal cells, and determining a difference in chirality between the sample cells and normal cells.Type: GrantFiled: June 15, 2012Date of Patent: May 1, 2018Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORKInventors: Gordana Vunjak-Novakovic, Leo Qun Wan
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Patent number: 9939353Abstract: An apparatus for cell observation and a method for cell collection using the same are disclosed. The apparatus for cell selection comprises a first substrate having an opening; and a second substrate having a photoresist unit disposed on a surface thereof, wherein the photoresist unit comprises at least one notch and defines a space which is interconnected with the notch and corresponds to the opening of the first substrate.Type: GrantFiled: March 25, 2015Date of Patent: April 10, 2018Assignee: NATIONAL TSING HUA UNIVERSITYInventors: Fan-Gang Tseng, Jui-Chia Chang, Tsung-Ju Chen
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Patent number: 9932645Abstract: The present invention relates to a method for detecting bacteriolytic conditions in a test sample comprising the step of contacting the test sample with a bacterial strain and a substrate which undergoes a detectable change when cleaved by a specific enzyme of said bacterial strain, wherein the bacterial strain is unable of: (a) uptaking the substrate from the medium and (b) secreting the enzyme specific for the substrate outside of the cell and wherein detecting a change in the test sample due to the cleavage of the substrate by its specific bacterial enzyme indicates that bacteriolytic conditions exist in the test sample. The invention further provides a kit for performing the method of the invention.Type: GrantFiled: November 12, 2014Date of Patent: April 3, 2018Assignee: UNIVERSITAT DE BARCELONAInventors: Maria Teresa Muniesa Pérez, Lejla Imamovic, Elisenda Ballesté Pau, Anicet Blanch Gisbert, Francisco Lucena Gutiérrez, Joan Jofre Torroella
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Patent number: 9933446Abstract: This disclosure is directed to information management techniques that may be used during automated processing of biological growth media. In one embodiment, a method comprises reading an identification element associated with a biological growth medium, identifying manufacturing information associated with the biological growth medium based on the identification element, wherein the manufacturing information includes one or more manufacturing variables measured from the biological growth medium when the biological growth medium was manufactured, adjusting one or more processing parameters associated with processing of the biological growth medium based on the manufacturing variables measured from the biological growth medium when the biological growth medium was manufactured, and processing the biological growth medium in an automated system based on the processing parameters.Type: GrantFiled: February 27, 2009Date of Patent: April 3, 2018Assignee: 3M Innovative Properties CompanyInventors: Phillip A. Bolea, Michael E. Hughes
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Patent number: 9932620Abstract: A rapid, sensitive method of separating and detecting microorganisms from a sample potentially containing microorganisms, such as but not limited to bacteria, fungi, yeast, viruses, and the like. The method relies on separation techniques to separate and concentrate the cells from the sample, together with chemical techniques to amplify the amount of detectable signal from low numbers of cells to provide a rapid and sensitive method of detecting microorganisms. This detection method may utilize: a filtration device; a centrifugation device; a system; a swab device; and kit comprising one or more of the devices and components to perform the present method of separating and detecting microorganisms in a sample potentially containing microorganisms. The sample may be a chemical, cosmetic, personal care, pharmaceutical, or consumable good in its raw material, in-process, and/or finished product states that needs to be tested for any contaminating microorganisms prior to shipment to the consumer.Type: GrantFiled: June 29, 2012Date of Patent: April 3, 2018Assignee: CELSIS LTD.Inventors: Subramani Sellappan, Andrew Hearn, Salvatore Seminara