Using Radioactive Material Patents (Class 435/35)
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Patent number: 4765972Abstract: Pharmacologically active, radioactive, and photoactive vinblastine analogs are provided which can be used to bind covalently to cellular polypeptides which have high affinity for Vinca alkaloids. The compounds are N-(p-azido[3,4-.sup.3 H]benzoyl)-N'beta-aminoethylvindesine and N-p-azido-[3-.sup.125 I]-salicyl-N'-beta-aminoethylvindesine. The compounds can be used to identify cellular Vinca alkaloid receptors which may be involved in antineoplastic, cytotoxic and drug resistant mechanisms of actions. In addition to specific interaction with tubulin, these compounds specifically bind to a 150-180 kDa surface membrane glycoprotein which is overexpressed in multidrug resistant cells.Type: GrantFiled: April 3, 1986Date of Patent: August 23, 1988Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Ahmad R. Safa, Ronald L. Felsted
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Patent number: 4755459Abstract: Monoclonal antibodies specific to Neisseria gonorrhoeae lipopolysaccharide components having no cross-reactivity with N. meningitidis have been produced and found useful in the diagnosis of the presence of N. gonorrhoeae.Type: GrantFiled: June 10, 1985Date of Patent: July 5, 1988Assignee: Canadian Patents and Development LimitedInventors: Terry W. Pearson, Malcolm B. Perry
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Patent number: 4755458Abstract: The present invention relates to a wide range of genetic analyses using the technique of nucleic acid hybridization. These genetic analyses include, for example, the diagnosis of infections by foreign microbes and the detection of specific genetic traits and abnormalities. More specifically, the present invention is related to the detection of the presence of a polynucleotide sequence of interest.Type: GrantFiled: August 30, 1984Date of Patent: July 5, 1988Assignee: Enzo Biochem, Inc.Inventors: Elazar Rabbani, Dean L. Engelhardt
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Patent number: 4753878Abstract: A technique for identifying an unknown microorganism, in which technique a specimen of the unknown microorganism is prepared to which a radioactive emissive agent has been added that is actively incorporated into the products of metabolism of the microorganism to produce a mix of radioactive peptide or protein emissive products in a manner that depends on the metabolic mechanism of the microorganism. The peptide or protein emissive products in the mix resulting from the preparation of the specimen are then separated. The separated emissive products are detected by sensing the emission from the products to provide an electrical signal whose wave pattern is indicative of the identity of the microorganism. This wave pattern is compared in a computer with patterns stored therein representing a collection of known microorganisms.Type: GrantFiled: May 2, 1985Date of Patent: June 28, 1988Assignee: AMB Systems Corp.Inventor: Robert E. Silman
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Patent number: 4752573Abstract: The use of pterins to increase the activity of lymphokines and other cell growth factors, and a diagnostic or theraupeutic preparation that contains pterins combined with lymphokines. Adding pterins to lymphokines can increase the activity of the lymphokines by 3 to 5 times.Type: GrantFiled: April 8, 1986Date of Patent: June 21, 1988Assignees: Biotest-Serum-Institut GmbH, Gesellschaft fur Strahlen-und Umweltforschung mbHInventors: Irmgard Ziegler, Udo Schwulera, Hans Sonneborn
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Patent number: 4743540Abstract: A method is devised to assay and diagnose common varied immunodeficiency syndrome. This syndrome is subdivided or subset into at least four separate groups of B-cell deficiencies based on a patients peripheral blood B-cell proliferative and/or differentiative response to various stimulatory factors alone or in combination. CVI diagnosis and therapy are aided by this invention.Type: GrantFiled: September 27, 1983Date of Patent: May 10, 1988Assignee: Memorial Sloan-Kettering Cancer CenterInventors: Peter Ralph, Osamu Saiki
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Patent number: 4743552Abstract: The invention concerns a method for the routine primary culture of human colonic epithelial cells within a monolayer. 12-0-tetradecanoylphorbol-13-acetate (TPA) enhanced DNA synthesis an average of 8 fold when assayed by [.sup.3 h]dThd labeling indices (L.I.) in colonic epithelial cells from familial polyposis patients and in tubular adenomas. No such stimulation by TPA was seen in cells from high-risk patients without familial polyposis, or in cells from low-risk subjects.Type: GrantFiled: September 26, 1984Date of Patent: May 10, 1988Assignee: Sloan-Kettering Institute for Cancer ResearchInventors: Eileen A. Friedman, Martin Lipkin
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Patent number: 4717653Abstract: A method of characterizing an unknown organism which comprises comparing the chromatographic pattern of restriction endonuclease-digester DNA from the unknown organism, which digested DNA has been hybridized or reassociated with ribosomal RNA information-containing nucleic acid from or derived from a known probe organizm, with at least two equivalent chromatographic patterns, each one of the equivalent chromatographic patterns defining a known different organism species; and establishing the species of the unknown organism by means of a conserved set of ribosomal RNA sequence-containing restriction fragments present in the chromatographic pattern of the unknown organism.Type: GrantFiled: September 25, 1981Date of Patent: January 5, 1988Inventor: John A. Webster, Jr.
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Patent number: 4713328Abstract: A method is disclosed for efficient, rapid diagnosis of bacterial infections by measuring for the presence of beta-lactamase using .sup.14 C-benzylpenicillin as an enzyme substrate and isolating cleaved substrate with an ion exchange column.Type: GrantFiled: January 3, 1984Date of Patent: December 15, 1987Inventor: Robert H. Yolken
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Patent number: 4652521Abstract: A method for the early diagnosis of Bordetella diseases based on the detection of Bordetella adenylate cyclase and the ability of this enzyme to catalyze the formation of cyclic adenosine monophosphate (cAMP). A sample secretion is taken from a suspected victim of a Bordetella disease (the most common of which in humans is whooping cough) and incubated under conditions to promote the formation of cAMP, which may then be measured as evidence of the presence of the disease. A sample of nasopharyngeal secretions is maintained in a nutrient medium pending assay. An adenylate cyclase assay is made by incubating the sample with adenosine triphosphate (ATP) and the calcium binding protein calmodulin to generate cAMP. The cAMP is measured by any of several known available methods.Type: GrantFiled: March 4, 1985Date of Patent: March 24, 1987Assignee: Regents of the University of MinnesotaInventors: Dennis L. Confer, John W. Eaton
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Patent number: 4634586Abstract: Leukocytes are radioimmunoimaged by injecting patients with an immunoreactive nonleukocidal conjugate of an anti-leukocyte antibody and a gamma-emitting radioactive metal chelate, waiting for the conjugate to localize on the leukocytes, injecting the patient with an antibody to the conjugate to clear the blood of background nonlocalized conjugate, and visualizing the leukocytes by scintillation scanning.Type: GrantFiled: May 21, 1984Date of Patent: January 6, 1987Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: David A. Goodwin, Claude F. Meares
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Patent number: 4609622Abstract: A fast growing, continuous, functional rat thyroid cell strain, FRTL-5, which maintains functional characteristics of iodide uptake and thyroglobulin synthesis over prolonged periods of culture is cloned from FRTL cells obtained from primary cultures of Fischer rat thyroid glands. The FRTL-5 cells are cultured in a medium containing approximately 5 percent calf serum supplemented with a mixture of hormones, at least one of which is thyrotropin.The FRTL-5 cells are employed in a series of assays which measure thyroid stimulatory or inhibitory factors. The FRTL-5 system of assay specifically measures thymidine incorporation, cAMP elevation and iodide uptake and permits the evaluation of patient sera, particularly those afflicted with Graves' disease and other autoimmune thyroid diseases, thereby providing a means for determining appropriate methods of treatment.Type: GrantFiled: May 31, 1983Date of Patent: September 2, 1986Assignees: Interthyr Research Foundation Inc., The University of MarylandInventors: Leonard D. Kohn, William A. Valente, Evelyn F. Grollman-Wolff, Salvatore M. Aloj, Paolo Vitti
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Patent number: 4608397Abstract: The invention relates to a process for the production of polyurethane(urea) compositions which contain lignite and/or peat bound in an abrasion-proof manner, which are preferably modified cationically and which have a very high water absorbability. The compositions are produced by reacting isocyanate-terminated prepolymers, which are preferably cationically modified, and which preferably have a functionality of more than 2.1, with more than the stoichiometric quantity of water in the presence of lignite and/or peat and optionally in the presence of organic and/or inorganic fillers and biomasses (living cells, living bacteria or enzymes). The corresponding polyurethane(urea) compositions contain up to 95% by weight of lignite and/or peat in the filler-containing polyurethane(urea) composition and are already swollen from production. The water absorbability (WAF) value thereof when suspended in water is from 33 to 97% by weight of water.Type: GrantFiled: November 16, 1984Date of Patent: August 26, 1986Assignee: Bayer AktiengesellschaftInventor: Artur Reischl
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Patent number: 4604351Abstract: The effect of a chemical on bacterial growth is rapidly determined by culturing a sample of bacteria in the presence of said chemical for a period of time sufficient to obtain detectable bacterial growth. During the culturing period, a labelled nucleotide is added to the growth media and the uptake of said nucleotide is measured and compared to a control.Type: GrantFiled: July 13, 1984Date of Patent: August 5, 1986Assignee: Bronx Lebanon HospitalInventor: Leonard Amaral
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Patent number: 4590158Abstract: In a system for monitoring fluid for microbial contamination, successive samples of the fluid to be monitored are aseptically directed periodically to a growth medium. A detector detects gaseous metabolic products evolving from the growth medium to provide an indication of incipient contamination of the fluid being monitored.Type: GrantFiled: September 4, 1981Date of Patent: May 20, 1986Inventor: Edward A. Eikman
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Patent number: 4526865Abstract: An identification technique for micro-organisms in which a dilute solution of a culture medium containing an unknown micro-organism has added thereto an emissive agent such as a radioactive amino acid to produce a mix of emissive products that depends on the metabolic mechanism of the micro-organism. After a predetermined incubation period, the reaction is arrested and the solution layered onto a gel plate where it is subjected to electrophoresis. The plate is then autoradiographed by exposing the gel to a sensitive photographic film for a period sufficient to produce thereon a characteristic band pattern functioning as an identifier for the micro-organism. Identification may be effected by comparing the identifier for the unknown with a collection of identifiers for known micro-organisms to find a match with one of these known identifiers.Type: GrantFiled: January 22, 1982Date of Patent: July 2, 1985Assignee: AMB Systems Corp.Inventor: Robert E. Silman
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Patent number: 4521512Abstract: A method for the identification of microorganisms comprises adding to a sample containing an unknown microorganism an emissive agent such as a radioactive amino acid to produce a mix of emissive products that depends on the metabolic mechanism of the microorganism. After incubation, the reaction is arrested and the emissive products are separated, as by electrophoresis on a gel plate. The plate may then be autoradiographed by exposure to a photographic film to produce on the latter a characteristic band pattern functioning as an identifier for the microorganism. Identification can be effected by comparing the identifier for the unknown with a collection of identifiers for known microorganisms to find a match with one of these known identifiers. The comparison may be carried out by scanning the unknown identifier to produce a signal which is compared with signals representing known identifiers stored in a computer.Type: GrantFiled: September 28, 1982Date of Patent: June 4, 1985Assignee: AMB Systems Corp.Inventor: Robert E. Silman
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Patent number: 4514509Abstract: A rapid, sensitive, and specific method for diagnosing Legionnaires' disease through the detection of antigens excreted by the patient and a method for preparing anti-Legionella immunoglobulin G for use in such diagnostic method.Type: GrantFiled: September 21, 1981Date of Patent: April 30, 1985Assignee: Indiana University FoundationInventors: Richard B. Kohler, Arthur C. White
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Patent number: 4508820Abstract: The invention provides an assay method for cellular and extracellular protein biodynamics comprising the use of radiochemical techniques in combination with high resolution protein separation procedures and recently developed silver stain techniques, to assess turnover of polypeptides over time. Quenching of polypeptide radiolables in silver stained gels is obviated by the use of .sup.14 C-labelled amino acids as precursors.Type: GrantFiled: February 27, 1984Date of Patent: April 2, 1985Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Carl R. Merril, David Goldman
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Patent number: 4492760Abstract: An assay for HLA D phenotype wherein antigen-pulsed monocytes are contacted with antigen-specific T lymphocytes or T cell hybridomas and the extent of selective binding between the pulsed monocytes and the T lymphocytes or T cell hybridomas determines HLA D phenotype of the monocyte. The assay is particularly useful for quickly typing donor tissue prior to transplantation.Type: GrantFiled: April 19, 1983Date of Patent: January 8, 1985Assignee: The Wistar Institute of Anatomy and BiologyInventor: Elaine C. DeFreitas
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Patent number: 4424201Abstract: A method for the detection of malignant leukocytic cells which comprises:contacting a fluid containing malignant leukocytic cells with a merocyanine dye of the formula: ##STR1## wherein n=1-5; X=0 or S; R.sub.1 and R.sub.2 are the same or different C.sub.2 -C.sub.5 alkyl groups:wherein said dye is incorporated into said cells; separating the cells from the incubation mixture; and detecting said dyed cells. Fluorescence or radioactivity detection methods are preferred detecting means.Type: GrantFiled: November 28, 1978Date of Patent: January 3, 1984Assignee: Rockefeller UniversityInventors: Jay E. Valinsky, Edward Reich, Thomas G. Easton
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Patent number: 4416995Abstract: Method, apparatus and automated system for determining the effects of a chemical agent upon a bacterial sample. Aliquots of a bacterial suspension in a culture medium, the chemical agent, and tritiated thymidine are deposited in polyethylene tubes having first and second compartments separated by a fixed millipore filter. A precipitation agent, such as trichloroacetic acid, is deposited into the polyethylene tubes to precipitate the bacteria. The bacteria is separated from the liquid by operation of a vacuum system which draws the liquid through the filter, thereby filtering out the bacteria. The filtered bacteria is subsequently washed with a washing solution. In one embodiment, a scintillation fluid is added to the bacteria, the bacteria then being conveyed to a scintillation counter which produces an output signal corresponding to the amount of radiation emanating from the bacteria.Type: GrantFiled: September 10, 1981Date of Patent: November 22, 1983Assignee: Bronx-Lebanon Hospital CenterInventor: Leonard Amaral
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Patent number: 4374120Abstract: An improved method of determining the nature of a substance by fluoroscence spectroscopy wherein a fluorescent marker is coupled to the molecules of the substance comprises the use of a marker having a longer period of fluorescence than those of possible sources of noise and by employing an exciting radiation pulse of short duration so that the fluorescence of the marker is detected after the objectionable sources of fluorescence have ceased; the marker including a fluorescent lanthanide chelate complex.Type: GrantFiled: March 7, 1980Date of Patent: February 15, 1983Assignee: Wallac OyInventors: Erkki Soini, Ilkka Hemmila
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Patent number: 4358535Abstract: Method and compositions for infectious disease diagnosis and epidemiology involving labeled nucleotide probes complementary to nucleic acid coding for a characteristic pathogen product. Clinical isolates are cultivated, expanding the number of microorganisms, the resulting colonies lysed, the genome normally denatured and then fixed. Alternatively, clinical samples (stool, sputum, pus, etc.) are spotted onto an inert support. The sample is treated in such a way that the DNA is liberated from microbes present in the sample and complexed onto the support. The DNA is normally denatured and fixed in this process. Subsequently, a labelled polynucleotide probe specific for a DNA sequence characteristic of a pathogenic product suspected of being present in the clinical sample is contacted with the fixed genomic single stranded nucleic acid under hybridizing conditions. Hybridization of probes to the single stranded nucleic acid is diagnostic of the presence of the pathogen.Type: GrantFiled: December 8, 1980Date of Patent: November 9, 1982Assignee: Board of Regents of the University of WashingtonInventors: Stanley Falkow, Stephen L. Moseley
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Patent number: 4246352Abstract: The present invention provides a test sample container for measuring the biological activity of cells, bacteria, or micro-organisms in a test sample by radiometric techniques. The container is suited for use with automated laboratory equipment and provides greatly improved measurement efficiency over prior designs. In one embodiment, the test sample container includes a serum vial adapted to contain a quantity of a test sample and a radionuclide-labeled nutrient media. A micro-tube, which contains a mixture of a gas absorber and a scintillation compound, is positioned above the surface of the sample/media mix by a split-skirt stopper that also serves to seal the serum vial. The gas metabolized or otherwise evolved by the micro-organisms in the test sample is absorbed by the absorber in the micro-tube causing the scintillation compound to emit light flashes at a rate proportional to the quantity of radioactive gas absorbed.Type: GrantFiled: December 18, 1978Date of Patent: January 20, 1981Inventor: Edward U. Buddemeyer
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Patent number: 4235866Abstract: Disclosed is a method for the total determination of hormones and pharmaceuticals partially bound to specific or non-specific proteins, by the enzymatic hydrolysis of the bonding proteins, the reaction of the hormones or pharmaceuticals with an antibody and the radioimmunological determination of the hormones and pharmaceuticals, characterized by the fact that the immobilized antibodies are used.Type: GrantFiled: April 24, 1978Date of Patent: November 25, 1980Assignee: Chandon Investment Planning Ltd.Inventor: Hans A. Thoma
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Patent number: 4178359Abstract: An improved method for immunoassay for antibodies to microbial organisms is disclosed. The improvement involves the use of diluted anti-human IgG to produce a non-visible agglutinate of the microbial antigen-serum antibody cojugate. Such method provides for maximum label binding and thus maximum assay sensitivity compared to the previously employed conjugate precipitation method. The present improvement is particularly useful in a radioimmunoassay for detecting antibodies to Neisseria gonorrhoeae (N.g.) or rubella virus in sera.Type: GrantFiled: March 15, 1978Date of Patent: December 11, 1979Assignee: Hoffmann-La Roche Inc.Inventors: Susan M. Mondabaugh, Magdalena U. Gomez