Method Of Altering The Differentiation State Of The Cell Patents (Class 435/377)
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Patent number: 10669529Abstract: Provided is a method for producing vascular endothelial cells from pluripotent stem cells, the method comprising the following steps (i) to (iii): (i) a step of culturing pluripotent stem cells in a culture medium comprising a BMP, on a culture vessel coated with a first matrix, to produce mesodermal progenitor cells; (ii) a step of dissociating the resulting cells into single cells; and (iii) a step of culturing the resulting cells in a culture medium comprising VEGF, on a culture vessel coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, type IV collagen and fibronectin.Type: GrantFiled: July 14, 2016Date of Patent: June 2, 2020Assignees: Kyoto University, Osaka UniversityInventors: Tatsutoshi Nakahata, Megumu Saito, Akira Niwa, Ryo Ota, Kiyotoshi Sekiguchi
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Patent number: 10655105Abstract: The present invention relates to a method for preparing highly pure pancreatic progenitor cells by using pluripotent stem cells such as ES cells or iPS cells as a source, inducing their differentiation into pancreatic progenitor cells, and culturing and proliferating the pancreatic progenitor cells. Specifically, the present invention relates to a method for proliferation of pancreatic progenitor cells, comprising the step of culturing the pancreatic progenitor cells in a medium containing (i) an EGF signal transduction activator and/or an FGF signal transduction activator and (ii) a ROCK inhibitor.Type: GrantFiled: August 3, 2015Date of Patent: May 19, 2020Assignee: Takeda Pharmaceutical Company LimitedInventor: Yuya Kunisada
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Patent number: 10632154Abstract: The present invention relates to the use of insulin-producing cells encapsulated in silanized hydroxypropyl methylcellulose (Si-HPMC) for the treatment of type 1 diabetes. Methods and kits are also provided for restoring and/or maintaining euglycemia in type 1 diabetic patients and in type 1 prediabetic patients.Type: GrantFiled: November 10, 2016Date of Patent: April 28, 2020Assignees: Ecole Nationale Veterinaire, Chu Nantes, Universite de Nantes, Institute National de la Sante et de la Recherche MedicaleInventors: Jean-Marie Bach, Mathilde Mosser, Apolline Salama, Anne Moure, Xavier Leveque, Pierre Weiss, Jérôme Guicheux, Cécile Boyer, David Riochet
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Patent number: 10626369Abstract: The present disclosure relates to methods of producing a preparation of CD140a/PDGFR? positive cells from pluripotent cells, where the preparation comprises oligodendrocyte progenitor cells co-expressing OLIG2 and CD140a/PDGFR?. The cell preparation has an in vivo myelination efficiency that is equal to or greater than the in vivo myelination efficiency of a preparation of A2B5+/PSA-NCAM? sorted fetal human tissue derived oligodendrocyte progenitor cells. Methods of enriching and therapeutic uses of the disclosed cell preparation are also described.Type: GrantFiled: February 8, 2017Date of Patent: April 21, 2020Assignee: University of RochesterInventors: Steven A. Goldman, Su Wang
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Patent number: 10626370Abstract: Process for carrying out an in vitro biochemical reaction for preparing a cell suspension suitable for application to the skin of a patient.Type: GrantFiled: March 22, 2018Date of Patent: April 21, 2020Assignee: LABORATOIRES GENEVRIERInventors: Dominique Vacher, Jean-Noel Gouze
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Patent number: 10612094Abstract: The present invention provides genetic markers for identifying engraftable human cardiac ventricular progenitor cells. The engraftment markers of the invention include angiogenic markers and extracellular matrix markers. Human ventricular progenitor cells expressing these markers are capable of forming ventricular tissue in vivo that is vascularized and supported by an extracellular matrix. Methods of engrafting human cardiac ventricular progenitor cells by transplanting into a subject progenitor cells that express the engraftment markers are also provided.Type: GrantFiled: February 15, 2017Date of Patent: April 7, 2020Assignee: Procella Therapeutics ABInventors: Chuen Yan Leung, Jonathan Clarke, Jiejia Xu, Federica Santoro, Makoto Sahara, Kenneth R. Chien
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Patent number: 10590390Abstract: The present invention relates to a method for preparing highly pure pancreatic progenitor cells by using pluripotent stem cells such as ES cells or iPS cells as a source, inducing their differentiation into pancreatic progenitor cells, and culturing and proliferating the pancreatic progenitor cells. Specifically, the present invention relates to a method for proliferation of pancreatic progenitor cells, comprising the step of culturing the pancreatic progenitor cells in a medium containing (i) an EGF signal transduction activator and/or an FGF signal transduction activator and (ii) a ROCK inhibitor.Type: GrantFiled: August 3, 2015Date of Patent: March 17, 2020Assignee: Takeda Pharmaceutical Company LimitedInventor: Yuya Kunisada
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Patent number: 10584313Abstract: The present invention relates generally to the field of somatic cell nuclear transfer (SCNT) and to the creation of cloned animals and cells. The disclosure relates to a method of cloning a mammal, obtaining pluripotent cells such as embryonic stem cells, or for reprogramming a mammalian cell using an oocyte and a fertilized embryo.Type: GrantFiled: July 23, 2014Date of Patent: March 10, 2020Assignee: Astellas Institute for Regenerative MedicineInventors: Robert P. Lanza, Young Gie Chung
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Compositions, systems, and methods for generating inner ear hair cells for treatment of hearing loss
Patent number: 10568883Abstract: Method and compositions for inducing the self-renewal of stem/progenitor supporting cells comprised by a cochlear cell population, including inducing the stem/progenitor cells to proliferate while maintaining, in the daughter cells, the capacity to differentiate into hair cells.Type: GrantFiled: September 3, 2015Date of Patent: February 25, 2020Assignees: MASSACHUSETTS INSTITUTE OF TECHNOLOGY, THE BRIGHAM AND WOMEN'S HOSPITAL, INC.Inventors: Jeffrey M. Karp, Robert S. Langer, Xiaolei Yin, Nitin Joshi -
Combination compositions and their use in methods for treating obesity and obesity-related disorders
Patent number: 10568852Abstract: The present invention relates to compositions comprising at least two different active compounds and the use of such combination compositions in medicine, in particular in methods for treating obesity and obesity-related disorders and/or in methods for inhibiting weight gain.Type: GrantFiled: May 23, 2016Date of Patent: February 25, 2020Assignee: HELMHOLTZ ZENTRUM MUNCHEN - DEUTSCHES FORSCHUNGSZENTRUM FUR GESUNDHEIT UND UMWELT (GMBH)Inventors: Timo Dirk Mueller, Matthias Tschoep, Christoffer Clemmensen, Brian Finan -
Patent number: 10527631Abstract: Methods and compositions are provided for the selective activation of a BMP-dependent response in certain cell types. Methods include identifying a ligand or ligand combinations as well as cell receptor profiles that result in selectively activating a ligand-dependent response through interactions with ligand receptors on a first cell type that do not activate the ligand-dependent response in a second cell type.Type: GrantFiled: December 19, 2017Date of Patent: January 7, 2020Assignee: California Institute of TechnologyInventors: Yaron E. Antebi, James Linton, Michael Elowitz, Heidi Klumpe
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Patent number: 10519422Abstract: The invention relates to a method of producing a retinal pigment epithelial cell from a human pluripotent stem cell, and a method of treating or preventing a retinal disease by using the produced cell. The retinal pigment epithelial cell is prepared by (a) inducing differentiation of a human pluripotent stem cell into a pigment cell by adhesion cultivation of a human pluripotent stem cell in a medium containing a Nodal signal inhibitor and a Wnt signal inhibitor in the absence of a feeder cell to give a culture containing the pigment cell, (b) subjecting the obtained culture to further adhesion culture to give a culture containing a pigment cell colony, and (c) isolating the pigment cell from the obtained culture and culturing the cell to give a retinal pigment epithelial cell.Type: GrantFiled: February 29, 2012Date of Patent: December 31, 2019Assignee: RIKENInventors: Masayo Takahashi, Satoshi Okamoto, Noriko Sakai
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Patent number: 10513685Abstract: Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 ?m to 500 ?m and a bottom area of 100 ?m2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).Type: GrantFiled: March 23, 2011Date of Patent: December 24, 2019Assignees: CORNING INCORPORATED, PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITYInventors: Yoko Itchoda, Go Tazaki, Masaya Hosoda, Motohiro Fukuda, Hideki Taniguchi, Yun-Wen Zheng, Keisuke Sekine
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Patent number: 10513688Abstract: Methods of generating cardiomyocytes from induced pluripotent stem cells (IPSCs) are provided. More specifically, the present disclosure relates to methods of generating cardiomyocytes from iPSCs using electrical stimulation. In some aspects, uses of such cells for therapeutics and in methods of treatment are provided.Type: GrantFiled: December 11, 2017Date of Patent: December 24, 2019Assignee: University of CincinnatiInventors: Yi-Gang Wang, Ha Won Kim, Ruilian Ma
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Patent number: 10501724Abstract: The present invention provides a method for producing a retinal progenitor cell, including (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, and (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway but containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells.Type: GrantFiled: August 22, 2014Date of Patent: December 10, 2019Assignees: SUMITOMO CHEMICAL COMPANY, LIMITED, RIKENInventors: Tokushige Nakano, Yoshiki Sasai, Chikafumi Ozone
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Patent number: 10494608Abstract: The present invention relates to a method for isolating bona fide pancreatic progenitor cells and to cell populations enriched for bona fide pancreatic progenitor cells.Type: GrantFiled: April 21, 2016Date of Patent: December 3, 2019Assignee: University of CopenhagenInventors: Jacqueline Ameri, Henrik Semb
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Patent number: 10472610Abstract: Provided is a method for generating pancreatic bud cells, having the step of culturing PDX1+/NKX6.1? cells in a medium containing KGF, EGF and a BMP inhibitor. The culturing step may be performed in suspension cultures or in adherent cultures. When the cells are cultured in adherent cultures, the cells may be cultured in a medium further containing a ROCK inhibitor or a nonmuscle myosin II inhibitor.Type: GrantFiled: May 20, 2015Date of Patent: November 12, 2019Assignee: KYOTO UNIVERSITYInventors: Kenji Osafune, Taro Toyoda
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Patent number: 10457916Abstract: It is an object of the present invention to provide a method for efficiently directing differentiation into insulin-producing cells in a xeno-free culture system.Type: GrantFiled: May 19, 2015Date of Patent: October 29, 2019Assignee: Tokyo Institute of TechnologyInventors: Shoen Kume, Nobuaki Shiraki
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Patent number: 10426799Abstract: Methods for production of platelets from pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are provided. These methods may be performed without forming embryoid bodies or clusters of pluripotent stem cells, and may be performed without the use of stromal inducer cells. Additionally, the yield and/or purity can be greater than has been reported for prior methods of producing platelets from pluripotent stem cells. Also provided are compositions and pharmaceutical preparations comprising platelets, preferably produced from pluripotent stem cells.Type: GrantFiled: December 21, 2013Date of Patent: October 1, 2019Assignee: Astellas Institute for Regenerative MedicineInventors: Qiang Feng, Shi-Jiang Lu, Robert P. Lanza
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Patent number: 10420803Abstract: Cell populations of intestinal midgut endoderm cells and methods of generating the cells expressing markers characteristic of intestinal endoderm lineage are disclosed. Methods of treating conditions such as diabetes are also disclosed.Type: GrantFiled: April 4, 2017Date of Patent: September 24, 2019Assignee: Janssen Biotech, Inc.Inventors: Sebastian Rieck, Alireza Rezania
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Patent number: 10421961Abstract: Some embodiments of the invention comprise methods, systems, and compositions to selectively induce, whether in vitro or in vivo, the neuronal differentiation of multipotent stromal cells through the application of microRNAs, including but not limited to miRNA-124, miRNA-137 and/or miRNA-9* expression products of those miRNAs, and molecules and compositions containing functional elements of those miRNAs. Some embodiments of the invention also comprise the therapeutic administration and use of such induced cells to treat mammalian injuries and diseases, including but not limited to, nervous system injuries or diseases that may otherwise result in decreased cell or system function.Type: GrantFiled: June 10, 2010Date of Patent: September 24, 2019Assignee: EXOSTEM BIOTEC LTDInventors: Chaya Brodie, Shimon Slavin
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Patent number: 10421942Abstract: Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types.Type: GrantFiled: February 13, 2017Date of Patent: September 24, 2019Assignee: ViaCyte, Inc.Inventors: Kevin Allen D'Amour, Alan D. Agulnick, Emmanuel E. Baetge
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Patent number: 10392602Abstract: Provided is a method of producing a lacrimal gland epithelial cell from a pluripotent stem cell, a lacrimal gland epithelial cell produced by the method, and a reagent kit for inducing differentiation from a pluripotent stem cell into a lacrimal gland epithelial cell. A method of producing a lacrimal gland epithelial cell includes the following step A: Step A: a step including increasing expression of Pax6 gene in a pluripotent stem cell or transfecting PAX6 protein thereinto, and increasing expression of Foxc1 gene or Foxp1 gene in the pluripotent stem cell or transfecting FOXC1 protein or FOXP1 protein thereinto.Type: GrantFiled: March 25, 2016Date of Patent: August 27, 2019Assignee: KEIO UNIVERSITYInventors: Masatoshi Hirayama, Minoru Ko
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Patent number: 10294449Abstract: A substrate for culturing cells that includes at least one fiber scaffold adapted to be contained within a disposable or non-disposable bioreactor, wherein the fiber scaffold further includes polymer fibers that have been created by electrospinning, and wherein the orientation of the fibers in the scaffold relative to one another is generally parallel, random, or both.Type: GrantFiled: August 20, 2013Date of Patent: May 21, 2019Assignee: NANOFIBER SOLUTIONS, LLCInventor: Jed K. Johnson
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Patent number: 10260041Abstract: The present invention relates generally to the field of cell biology of stem cells, more specifically the directed differentiation of pluripotent or multipotent stem cells, including human embryonic stem cells (hESC), somatic stem cells, and induced human pluripotent stem cells (hiPSC) using novel culture conditions. Specifically, methods are provided for obtaining neural tissue, floor plate cells, and placode including induction of neural plate development in hESCs for obtaining midbrain dopamine (DA) neurons, motorneurons, and sensory neurons. Further, neural plate tissue obtained using methods of the present inventions are contemplated for use in co-cultures with other tissues as inducers for shifting differentiation pathways, i.e. patterning.Type: GrantFiled: January 30, 2014Date of Patent: April 16, 2019Assignee: MEMORIAL SLOAN KETTERING CANCER CENTERInventors: Stuart Chambers, Lorenz Studer
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Patent number: 10226487Abstract: The present invention relates to methods for deriving multipotent Isl1+ cells (i.e. methods for inducing a cell to enter a multipotent Isl1+ lineage), methods for differentiating Isl1+ cells to cardiac cells, cells obtainable by such methods, kits and compositions for carrying out the methods in accordance with the invention, and also medical applications and pharmaceutical compositions of said cells.Type: GrantFiled: July 11, 2013Date of Patent: March 12, 2019Assignee: IsletOne ABInventors: Karl-Henrik Grinnemo, Oscar Simonson, Matthias Corbascio, Christer Sylvén, Eva Wärdell
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Patent number: 10202579Abstract: The present invention relates to the directed differentiation of mammalian pluripotent stem cells, especially human pluripotent stem (hPS) cells, into endodermal cells. In particular, the present invention relates to the treatment of mammalian pluripotent stem cells, especially hPS cells, with a DNA demethylating agent while undergoing differentiation into endodermal. The inventors have, as disclosed herein, found that exposing differentiating mammalian pluripotent stem cells, especially hPS cells, to a DNA demethylating agent leads to an improved morphology and improved yield of endodermal cells. The treatment with a DNA de-methylating agent also leads to a significant down-regulation of expression of the stem cell marker Oct4 and to an improved expression of endoderm specific markers, notably sox17, cxcr4 and hhex.Type: GrantFiled: November 28, 2013Date of Patent: February 12, 2019Assignee: Takara Bio Europe ABInventors: Barbara Küppers-Munther, Josefina Edsbagge
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Patent number: 10202580Abstract: The present invention provides a method for producing adult oligodendrocyte progenitor cells from proliferative oligodendrocyte progenitor cells, and a pharmaceutical composition having for an active ingredient thereof adult OPC produced according to that method. The method for producing adult OPC of the present invention is characterized by inducing proliferating OPC to differentiate into adult OPC by culturing in the presence of a ligand of a thyroid hormone receptor or retinoic acid receptor in a low oxygen environment. The present invention further provides adult OPC produced according to the production method of the present invention, and a pharmaceutical composition having these adult OPC as an active ingredient thereof.Type: GrantFiled: January 21, 2015Date of Patent: February 12, 2019Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Makoto Suematsu, Yasuhito Tokumoto, Shinpei Tamaki
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Patent number: 10190096Abstract: Disclosed herein are methods for generating SC-? cells, and isolated populations of SC-? cells for use in various applications, such as cell therapy.Type: GrantFiled: December 18, 2015Date of Patent: January 29, 2019Assignee: President and Fellows of Harvard CollegeInventors: Douglas A. Melton, Jeffrey R. Millman
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Patent number: 10160950Abstract: Methods for expanding proliferating populations of neuronal subtype-specific progenitors and creating substantially pure populations of motor neurons are provided herein. In particular, the present invention provides methods for maintaining the unique gene profile and differentiation potential of neuronal subtype-specific progenitors, such as motor neuron progenitors and hindbrain serotonergic neural progenitors.Type: GrantFiled: February 5, 2016Date of Patent: December 25, 2018Assignee: Wisconsin Alumni Research FoundationInventors: Su-Chun Zhang, Zhongwei Du
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Patent number: 10100284Abstract: A synthetic peptide having a stem cell differentiation-inducing activity to induce differentiation of pluripotent stem cells into endodermal cells, a stem cell differentiation inducer having this peptide as an active ingredient, and a method for inducing differentiation of pluripotent stem cells using these. The synthetic peptide provided by the present invention contains a stem cell differentiation-inducing peptide sequence, and this stem cell differentiation-inducing peptide sequence is selected from (1) an amino acid sequence constituting a signal peptide in any of amyloid precursor proteins (APP), amyloid precursor-like protein (APLP) 1 and APLP2, which are known as proteins belonging to the APP family, (2) a partial amino acid sequence constituting the signal peptide, or (3) a modified amino acid sequence formed by substitution, deletion and/or addition of 1, 2 or 3 amino acid residues in these amino acid sequences.Type: GrantFiled: December 24, 2014Date of Patent: October 16, 2018Assignee: TOAGOSEI CO. LTD.Inventors: Kenichi Tanaka, Tetsuhiko Yoshida, Nahoko Baileykobayashi
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Patent number: 10081792Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.Type: GrantFiled: December 31, 2015Date of Patent: September 25, 2018Assignee: Wisconsin Alumni Research FoundationInventors: James A. Thomson, Nicholas E. Propson, Michael P. Schwartz, Zhonggang Hou, Gene I. Uenishi, Igor I. Slukvin, William L. Murphy, Jue Zhang
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Patent number: 10077428Abstract: The present invention provides populations of human cardiovascular progenitor cells, methods of making such cells, and methods of using the cells for production of populations of cardiovascular colonies and populations of cardiomyocytes. Methods of cardiomyocytes replacement therapy are also provided.Type: GrantFiled: March 25, 2009Date of Patent: September 18, 2018Assignee: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAIInventors: Gordon Keller, Lei Yang, Steven Kattman
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Patent number: 10077429Abstract: The present invention provides a method of improving iPS cell establishment efficiency, comprising contacting a protein involved in primitive streak (PrS) formation, preferably Foxh1, or a nucleic acid that encodes the same with a somatic cell in a nuclear reprogramming step. Also provided is a method of producing an iPS cell, comprising contacting the protein involved in PrS formation or a nucleic acid that encodes the same, and nuclear reprogramming substance(s) with a somatic cell.Type: GrantFiled: October 23, 2013Date of Patent: September 18, 2018Assignee: Kyoto UniversityInventors: Kazutoshi Takahashi, Koji Tanabe, Shinya Yamanaka
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Patent number: 10072249Abstract: The purpose of the present invention is to provide a process or method that can be utilized when deriving a three-dimensional structure of a kidney from pluripotent stem cells such as ES cells or iPS cells. The differentiation inducing method is characterized by culturing pluripotent cells with the following three steps, in order: (a) a step of culturing an embryoid body induced from the pluripotent stem cells in medium containing Bmp4 and a high-concentration (concentration A) Wnt agonist; (b) a step of culturing the embryoid body in medium which contains activin, Bmp4, retinoic acid and a middle-concentration (concentration B) Wnt agonist; and (c) a step of culturing the embryoid body in medium containing Fgf9 and a low-concentration (concentration C) Wnt agonist (herein, the Wnt agonist concentrations is concentration A>concentration B>concentration C).Type: GrantFiled: October 16, 2014Date of Patent: September 11, 2018Assignee: NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITYInventors: Ryuichi Nishinakamura, Atsuhiro Taguchi
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Patent number: 10072243Abstract: This present invention provides novel methods for deriving embryonic stem cells and embryo-derived cells from an embryo without requiring destruction of the embryo. The invention further provides cells and cell lines derived without embryo destruction, and the use of the cells for therapeutic and research purposes. It also relates to novel methods of establishing and storing an autologous stem cell line prior to implantation of an embryo, e.g., in conjunction with reproductive therapies such as IVF.Type: GrantFiled: March 2, 2017Date of Patent: September 11, 2018Assignee: Astellas Institute for Regenerative MedicineInventors: Young Gie Chung, Robert P. Lanza, Irina V. Klimanskaya
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Patent number: 10011819Abstract: Provided are a culture medium for preparing neural stem cell and use thereof, the culture medium for preparing neural stem cell comprising: a basic culture medium suitable for the growth of stem cell, and a cell signal pathway inhibitor selected from at least one of GSK inhibitor, MEK inhibitor, TGF-? inhibitor, ROCK inhibitor and BMP inhibitor.Type: GrantFiled: February 6, 2013Date of Patent: July 3, 2018Assignee: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCESInventors: Guangjin Pan, Duanqing Pei, Lihui Wang, Linli Wang, Yanting Xue
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Patent number: 9994825Abstract: Described are methods for differentiating mammalian pluripotent stem cells towards a population of cells that can self-organize into vascular networks in vitro when harvested cells of the present invention are embedded into a hydrophilic matrix such as a hydrogel.Type: GrantFiled: March 17, 2014Date of Patent: June 12, 2018Assignee: The Johns Hopkins UniversityInventors: Sharon Gerecht, Sravanti Kusuma
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Patent number: 9909161Abstract: A method and system for evaluating the competence for transplantation of a graft sample containing cells capable of multinucleation are provided. In one form, the method includes determining multinucleating ability as an indicator representing the capacity for multinucleation of the cells capable of multinucleation.Type: GrantFiled: February 14, 2013Date of Patent: March 6, 2018Assignee: TERUMO KABUSHIKI KAISHAInventor: Kenta Sakamoto
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Patent number: 9822342Abstract: The present invention provides a method for efficiently producing cardiomyocytes from pluripotent stem cells, which method comprises the steps of dissociating embryoid bodies obtained during the production process, and allowing reaggregation of the resulting cells to allow formation of embryoid bodies.Type: GrantFiled: May 9, 2014Date of Patent: November 21, 2017Assignee: KYOTO UNIVERSITYInventors: Shinya Yamanaka, Yoshinori Yoshida, Kenji Miki
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Patent number: 9771558Abstract: The invention relates in certain embodiments to a method for generating dendritic cells by employing temperatures below 37° C. during the development of progenitor cells and immature dendritic cells. In some embodiments the invention relates to populations of dendritic cells and its use.Type: GrantFiled: December 7, 2006Date of Patent: September 26, 2017Assignee: DANDRIT DIOTECH A/SInventors: Alexei Kirkin, Karine Djandjougazian, Jesper Zeuthen
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Patent number: 9765292Abstract: A bioreactor for preferably three-dimensional cell culturing comprises a scaffold chamber, a first tube, a second tube and a first valve with a scaffold adapter, a tube adapter and a medium adapter. The first valve has a housing with a longitudinal female portion ending in an opening and a longitudinal actuator being arranged through the opening of the female portion of the housing such that the actuator is arranged partially inside the housing and partially outside the housing, wherein the actuator of the first valve is axially moveable relative to the housing of the first valve between a first position in which the first valve is in the operation position and a second position in which the first valve is in the medium change position. By providing the actuators in the first valve which is applied by axial movements, operation of the bioreactor can be comparably simple and safe.Type: GrantFiled: June 4, 2013Date of Patent: September 19, 2017Assignees: University Hospital of Basel, Cellec Biotek AGInventors: Elia Piccinini, David Wendt, Adam Papadimitropoulos, Laura Spinelli, Stefania Adele Riboldi, Francesco Giovanni Greco, Peter Iwatschenko
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Patent number: 9765297Abstract: The present invention relates to culture systems comprising differentiated stem cells, that may be used for identifying agents useful in treating degenerative nervous system disorders and are suitable for high-throughput screening applications. It is based, at least in part, on the discovery that co-cultures of (i) astrocytes expressing a mutated SODI gene and (ii) stem-cell derived motor neurons manifested cell death via a Bax-dependent mechanism, and modeled motor neuron death in amyotrophic lateral sclerosis.Type: GrantFiled: April 10, 2008Date of Patent: September 19, 2017Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORKInventors: Serge Przedborski, Hynek Wichterle, Makiko Nagai, Thomas M. Jessell
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Patent number: 9752125Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells expressing markers characteristic of the pancreatic endoderm lineage, wherein greater than 50% of the cells in the population co-express PDX1 and NKX6.1.Type: GrantFiled: May 11, 2011Date of Patent: September 5, 2017Assignee: Janssen Biotech, Inc.Inventor: Jean Xu
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Patent number: 9744195Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells. The present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer.Type: GrantFiled: June 26, 2015Date of Patent: August 29, 2017Assignee: LifeScan, Inc.Inventor: Jean Xu
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Patent number: 9738906Abstract: An object of the present invention is to provide a method of promoting polyploidization of megakaryocytes and thereby producing highly polyploidized megakaryocytes, a method of efficiently producing platelets from polyploidized megakaryocytes, and the like. The present invention provides a method of producing polyploidized megakaryocytes comprising a step of forcing expression of an apoptosis suppressor gene in megakaryocytes before polyploidization and culturing the resulting cells.Type: GrantFiled: May 11, 2012Date of Patent: August 22, 2017Assignee: The University of TokyoInventors: Koji Eto, Hiromitsu Nakauchi, Naoya Takayama, Sou Nakamura
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Patent number: 9700531Abstract: The present invention relates to a composition comprising metformin as an active ingredient for preventing or treating inflammatory bowel disease. The metformin compound or the metformin-etanercept (product name: Enbrel) composite according to the present invention may have excellent effects of maintaining the thickness of the small intestine and length of the large intestine normal, inhibiting or decreasing the activity of IL-17 and TNF-a, and promoting or increasing the activity of IFNr, and therefore can be effectively used as a pharmaceutical composition for preventing or treating autoimmune diseases including inflammatory bowel disease.Type: GrantFiled: March 19, 2015Date of Patent: July 11, 2017Assignee: Catholic University Industry Academic Cooperation FoundationInventors: Mi-La Cho, Seon-Yeong Lee, Eun-Ji Yang, Hye-Jin Son, Eun-Kyung Kim, Jun-Geol Ryu
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Patent number: 9657272Abstract: The present invention is directed towards methods of culturing non-keratinocyte epithelial cells, with the methods comprising culturing non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium while inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing.Type: GrantFiled: September 26, 2014Date of Patent: May 23, 2017Assignee: Georgetown UniversityInventors: Richard Schlegel, Xuefeng Liu
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Patent number: 9605243Abstract: Disclosed herein are reagent-cell complexes comprising one or more definitive endoderm cells. Also described herein are compositions for detecting definitive endoderm. Method of enriching, isolating and/or purifying definitive endoderm cells are also disclosed.Type: GrantFiled: October 29, 2013Date of Patent: March 28, 2017Assignee: ViaCyte, Inc.Inventors: Kevin Allen D'Amour, Alan D. Agulnick, Emmanuel Edward Baetge
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Patent number: 9593310Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells expressing markers characteristic of the pancreatic endocrine lineage that co-express NKX6.1 and insulin and minimal amounts of glucagon.Type: GrantFiled: August 31, 2015Date of Patent: March 14, 2017Assignee: Janssen Biotech, Inc.Inventor: Jean Xu