Culture, Maintenance, Or Preservation Techniques, Per Se Patents (Class 435/420)
  • Publication number: 20130102076
    Abstract: Methods and apparatus for promoting the growth of an aquatic photosynthetic organism within a growth medium in a photobioreactor may use a luminescent material targeting the aquatic photosynthetic organism in the photobioreactor. The luminescent material may be a substrate with a matrix of conductors coupled to the substrate, and light emitting diodes (“LEDs”) electrically coupled to the matrix of conductors. The aquatic photosynthetic organism in the photobioreactor is exposed to the light emitted by the LEDs.
    Type: Application
    Filed: October 24, 2012
    Publication date: April 25, 2013
    Inventors: Jason D. Licamele, Thomas J. Kulaga
  • Patent number: 8426702
    Abstract: The invention provides seed and plants of pepper hybrid PS 11446271 and the parent lines thereof, such as inbred parent line HAP 114-1128. The invention thus relates to the plants, seeds and tissue cultures of pepper hybrid PS 11446271 and the parent lines thereof, and to methods for producing a pepper plant produced by crossing such plants with themselves or with another pepper plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.
    Type: Grant
    Filed: August 16, 2010
    Date of Patent: April 23, 2013
    Assignee: Seminis Vegetable Seeds, Inc.
    Inventor: Terry G. Berke
  • Patent number: 8415528
    Abstract: The invention provides seed and plants of the pea line designated EX 08570956. The invention thus relates to the plants, seeds and tissue cultures of pea line EX 08570956, and to methods for producing a pea plant produced by crossing a plant of pea line EX 08570956 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line EX 08570956, including the seed, pod, and gametes of such plants.
    Type: Grant
    Filed: March 15, 2011
    Date of Patent: April 9, 2013
    Assignee: Seminis Vegetable Seeds, Inc.
    Inventors: David M. Webster, Charlene M. Webster
  • Patent number: 8415538
    Abstract: The invention provides seed and plants of pepper hybrid PS16364674 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of pepper hybrid PS16364674 and the parent lines thereof, and to methods for producing a pepper plant produced by crossing such plants with themselves or with another pepper plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.
    Type: Grant
    Filed: September 23, 2010
    Date of Patent: April 9, 2013
    Assignee: Seminis Vegetable Seeds, Inc.
    Inventor: Brian Just
  • Patent number: 8415529
    Abstract: The invention provides seed and plants of the pea line designated DLSC7V0955. The invention thus relates to the plants, seeds and tissue cultures of pea line DLSC7V0955, and to methods for producing a pea plant produced by crossing a plant of pea line DLSC7V0955 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line DLSC7V0955, including the seed, pod, and gametes of such plants.
    Type: Grant
    Filed: March 15, 2011
    Date of Patent: April 9, 2013
    Assignee: Seminis Vegetable Seeds, Inc.
    Inventor: David M. Webster
  • Publication number: 20130086702
    Abstract: The present invention provides manufacturing processes for biological replication of undifferentiated plant and animal cells and tissue in a weightless condition, including those systems used in current stem cell research and development and use of undifferentiated parenchyma in plants. The present invention further provides methods for adapting plants and animals to survive outside their native environments. In particular, undifferentiated cells from plants or animals are replicated under weightless conditions in which cell replication or proliferation is accelerated and sustained. Under such conditions, the undifferentiated cells can be “forced” to express sets of genes useful for survival in particular environmental conditions. In this manner, cells surviving prolonged exposure to specific environmental conditions can be selected for and cultivated to produce an organism adapted to that particular environment in an accelerated manner.
    Type: Application
    Filed: July 14, 2011
    Publication date: April 4, 2013
    Applicant: Zero Gravity, Inc.
    Inventor: John W. Kennedy
  • Patent number: 8404932
    Abstract: The invention provides seed and plants of the pea line designated EX 08570935. The invention thus relates to the plants, seeds and tissue cultures of pea line EX 08570935, and to methods for producing a pea plant produced by crossing a plant of pea line EX 08570935 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line EX 08570935, including the seed, pod, and gametes of such plants.
    Type: Grant
    Filed: March 15, 2011
    Date of Patent: March 26, 2013
    Assignee: Seminis Vegetable Seeds, Inc.
    Inventors: David M. Webster, Charlene M. Webster
  • Patent number: 8399742
    Abstract: The invention provides seed and plants of squash hybrid PX 13067464 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of squash hybrid PX 13067464 and the parent lines thereof, and to methods for producing a squash plant produced by crossing such plants with themselves or with another squash plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.
    Type: Grant
    Filed: April 21, 2010
    Date of Patent: March 19, 2013
    Assignee: Seminis Vegetable Seeds, Inc.
    Inventor: William C. Johnson
  • Publication number: 20130065306
    Abstract: A method for the culture of somatic plant embryos comprising contacting said embryos with a culture medium containing a plasmolytic or near plasmolytic concentration of a sugar alcohol or a combination of sugar alcohols; and culturing said embryos in said culture medium for a time period suitable for the desired level of maturation to be achieved. Inositol is a preferable sugar alcohol.
    Type: Application
    Filed: March 29, 2011
    Publication date: March 14, 2013
    Inventors: Ulrika Egertsdotter, David Clapham
  • Patent number: 8395020
    Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
    Type: Grant
    Filed: August 31, 2007
    Date of Patent: March 12, 2013
    Assignee: Monsanto Technology LLC
    Inventors: Jyoti R. Rout, Brenda A. Lowe, John Purcell, Anne Spelletich, Michael Spencer, Melissa Way
  • Publication number: 20130059339
    Abstract: A bioreactor (1) for the culture of cells (C) comprising a stack of carriers (7) for cell (C) adherence and liquid medium (M) distribution. The carriers (7) are stacked so as to define levels (6) between adjacent carriers (7) for the flow of the liquid medium (M). Adjacent levels (6) are fluidly interconnected via open spaces (2) so that the liquid medium (M) can flow from one level (6) to an adjacent level (6). The open spaces (2) between a first and an adjacent second level (6?) do not overlap with the one or more open spaces (2) between the second level (6?) and an adjacent third level (6?). One or more of the carriers may also include an area adapted to prevent cell adhesion or growth, thereby allowing for the viewing of cell growth on adjacent carriers from a vantage point external to the bioreactor. Related methods are also disclosed.
    Type: Application
    Filed: May 11, 2011
    Publication date: March 7, 2013
    Inventors: Didier Karerangabo, Julien Cardon, Jose Antonio Castillo Gonzalez
  • Patent number: 8383057
    Abstract: A probe array substrate suitable for forming a probe array that has high packing density, that can hold sufficient amounts of probe molecules, and that has little variation in the amounts of probe molecules. A plurality of arrayed probe-holding portions are defined by recesses, and isolating grooves are formed between the adjacent probe-holding portions to prevent probe solutions introduced into the probe-holding portions from spreading to adjacent probe-holding portions. Inner surfaces of the probe-holding portions are made hydrophilic, whereas inner surfaces of the isolating grooves are made hydrophobic. Liquid-introducing protrusions are formed in the probe-holding portions.
    Type: Grant
    Filed: May 13, 2010
    Date of Patent: February 26, 2013
    Assignee: Murata Manufacturing Co., Ltd.
    Inventor: Teruhisa Shibahara
  • Patent number: 8378175
    Abstract: The invention provides seed and plants of the pea line designated EX 08250826. The invention thus relates to the plants, seeds and tissue cultures of pea line EX 08250826, and to methods for producing a pea plant produced by crossing a plant of pea line EX 08250826 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line EX 08250826, including the seed, pod, and gametes of such plants.
    Type: Grant
    Filed: January 9, 2011
    Date of Patent: February 19, 2013
    Assignee: Seminis Vegetable Seeds, Inc.
    Inventor: Alexis Plouy
  • Patent number: 8378174
    Abstract: The invention provides seed and plants of the pea line designated 08560906. The invention thus relates to the plants, seeds and tissue cultures of pea line 08560906, and to methods for producing a pea plant produced by crossing a plant of pea line 08560906 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line 08560906, including the seed, pod, and gametes of such plants.
    Type: Grant
    Filed: September 22, 2010
    Date of Patent: February 19, 2013
    Assignee: Seminis Vegetable Seeds, Inc.
    Inventor: Charlene M. Webster
  • Publication number: 20130034899
    Abstract: A cell culturing device includes: an electrode on a surface of which a hydrophilicity/hydrophobicity converting material adapted to be electrically changed between hydrophilicity and hydrophobicity is provided. In this case, the electrode is disposed within a region in which a cell to be cultured is adapted to be accommodated. With the cell culturing device, the application of the suitable voltage to the electrode changes the hydrophilicity/the hydrophobicity of the hydrophilicity/hydrophobicity converting material. Thus, the feed material adsorbed to the hydrophilicity/hydrophobicity converting material is desorbed, thereby making it possible to feed the feed material to the cell.
    Type: Application
    Filed: July 18, 2012
    Publication date: February 7, 2013
    Applicant: Sony Corporation
    Inventors: Ichiro Takemura, Tomohiro Hayakawa, Eriko Matsui
  • Publication number: 20130004936
    Abstract: The present invention discloses a supercooling promoting agent comprising a tannin for producing practical water which does not freeze. As the tannin, a hydrolyzable tannin such as 2,3,6-tri-O-galloyl-?,?-D-hamamelose, 1,2,6-tri-O-galloyl-?-D-glucose, and a vitrification liquid, each of which contains the supercooling promoting agent are useful as a solution or the like for storing a biological material at low temperature.
    Type: Application
    Filed: March 3, 2011
    Publication date: January 3, 2013
    Applicant: National University Corporation Hokkaido Universit
    Inventors: Seizo Fujikawa, Yukiharu Fukiharu, Keita Arakawa, Hiroshi Nishioka
  • Publication number: 20120329158
    Abstract: A method of preparation of a plant tissue fragment is provided wherein apical dominance of plant meristematlc tissue is inhibited followed by fragmentation of the tissue. Also provided are methods of plant micropropagation and methods of artificial seed production using apical dominance suppression in preferably, a semi-automated process. Also provided is a plant tissue processing machine that generates plant fragments with high regeneration efficiency and an artificial seed production apparatus.
    Type: Application
    Filed: January 13, 2011
    Publication date: December 27, 2012
    Inventors: Prakash Lakshmanan, Angela Mordocco
  • Patent number: 8338143
    Abstract: This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known Taxus species, e.g., brevifolia, canadensis, cuspidata, baccata, globosa, floridana, wallichiana, media and chinensis. Particular modifications of culture conditions (i.e., media composition and operating modes) have been discovered to enhance the yield of various taxanes from cell culture of all species of Taxus. Particularly preferred enhancement agents include silver ion or complex, jasmonic acid (especially the methyl ester), auxin-related growth regulators, and inhibitors of the phenylpropanoid pathway, such as 3,4-methylenedioxy-6-nitrocinnamic acid. These enhancement agents may be used alone or in combination with one another or other yield-enhancing conditions. While the yield of taxanes from plant cell culture of T.
    Type: Grant
    Filed: December 17, 2010
    Date of Patent: December 25, 2012
    Assignee: Phyton Holdings, LLC
    Inventors: Venkataraman Bringi, Prakash Kadkade, Christopher Prince, Braden Roach
  • Publication number: 20120309090
    Abstract: A compressible bioreactor for cultivating photosynthetic microorganisms, the bioreactor comprising a feeder trough, a collection trough, a growth fabric, and a barrier layer, where the bioreactor has a compressed mode and an extended mode, the growth fabric is coupled to the feeder trough and the collection trough, the growth fabric is substantially extended in the extended mode of the bioreactor, the growth fabric is substantially compressed in the compressed mode of the bioreactor and the barrier layer is coupled to the feeder base and the collection base that encases the growth fabric in a substantially airtight environment
    Type: Application
    Filed: June 1, 2012
    Publication date: December 6, 2012
    Applicant: PROTERRO, INC.
    Inventors: John Aikens, Robert J. Turner, Denise L. Holzle
  • Publication number: 20120301411
    Abstract: Disclosed herein is a topical composition that contains (a) a plant meristem conditioned nutrient medium derived from the plants of the genus Oryza; (b) a preservative present at an amount sufficient to provide sterilizing or biostatic efficacy with respect to component (a); and (c) a dermatologically acceptable vehicle. When topically applied to mammalian cells, the composition of the invention can influence the epigenetic methylation of the DNA such that the methylation state is modulated to have more characteristics and patterns of the epigenetic state of younger cells.
    Type: Application
    Filed: March 27, 2012
    Publication date: November 29, 2012
    Inventors: Philip Ledette Ludwig, James Vincent Gruber
  • Patent number: 8313946
    Abstract: The present invention relates to methods for developing embryos and producing germination-competent embryos using a liquid embryo development media.
    Type: Grant
    Filed: September 5, 2007
    Date of Patent: November 20, 2012
    Assignee: Arborgen Inc.
    Inventors: Mike Becwar, John Clark, LeShaun Swinton, Narender Nehra, Tim Stout
  • Publication number: 20120284872
    Abstract: The present invention provides polynucleotides and related polypeptides of the protein AMT. The invention provides genomic sequence for the AMT gene. AMT is responsible for controlling nitrogen utilization efficiency in plants.
    Type: Application
    Filed: July 17, 2012
    Publication date: November 8, 2012
    Applicant: Pioneer Hi Bred International Inc.
    Inventors: RAJEEV GUPTA, Juan Liu, Kanwarpal S. Dhugga, Carl R. Simmons
  • Patent number: 8304239
    Abstract: This invention provides an efficient process for in vitro propagation of Jatropha through direct regeneration of leaf disc without any intermediary callus phase. This process provides a commercially viable process for producing true-to-type plants of Jatropha on a large scale and also as a method to produce transgenics through Agrobacterium-mediated transformation.
    Type: Grant
    Filed: March 30, 2007
    Date of Patent: November 6, 2012
    Assignee: Reliance Life Sciences PVT Ltd.
    Inventors: Tangirala S. Johnson, Ajay C. Deore
  • Publication number: 20120276633
    Abstract: There is provided a process for growing a phototrophic biomass in a reaction zone. The process includes treating an operative carbon dioxide supply-comprising gaseous material feed so as to effect production of a carbon dioxide-rich product material. The carbon dioxide concentration of the carbon dioxide-rich product material is greater than the carbon dioxide concentration of the operative carbon dioxide supply-comprising gaseous material feed. Production of at least a fraction of the operative carbon dioxide supply-comprising gaseous material feed is effected by a gaseous exhaust material producing process. At least a fraction of the carbon dioxide-rich product material is supplied to the reaction zone so as to effect growth of the phototrophic biomass by photosynthesis in the reaction zone.
    Type: Application
    Filed: April 27, 2011
    Publication date: November 1, 2012
    Applicant: POND BIOFUELS INC.
    Inventors: Jaime A. Gonzalez, Max Kolesnik, Steven C. Martin
  • Publication number: 20120276634
    Abstract: Described herein are methods and media for facilitating somatic embryogenesis and for collecting, conditioning, and transferring the washed embryos onto a substrate and into an environment suitable for conditioning the embryos for a desired period of time so they become germination-competent for plant production. The described plant embryo cleaning apparatus and method are used for preparing multiple plant embryos for plant production. The apparatus and method can use a cleaning fluid source, a fluid-conditioning system, a fluid-delivery structure, a cleaning station, an outlet mechanism, a negative pressure source, and a controller.
    Type: Application
    Filed: July 9, 2012
    Publication date: November 1, 2012
    Inventors: John Joseph CLARK, Narender Singh NEHRA, Mark Russell RUTTER, Jessica S. SAGE, Sydney Keith SEYMOUR, Timothy Joel STOUT, George SURRITTE, Ronald W. WINKLES
  • Patent number: 8281515
    Abstract: Methods for increasing the growth and biomass of algae and algae lipids by growing the algae in the presence of a polycationic substance such as soluble or insoluble chitosan and/or chitin are disclosed. Also disclosed are methods of harvesting algae from an aqueous environment by growing the algae in aggregated clumps and/or mats (formed by the inclusion of soluble or insoluble chitosan and/or chitin or other polycationic or cationic substances) that can be easily removed from the aqueous environment either by filtration, surface skimming, and/or growing in a porous containment device, such as a natural or synthetic fabric, and removing the fabric from the aqueous environment containing the aggregated algae. The methods disclosed have direct applications in biofuel and energy production, agricultural feedstock production, nutrient production, greenhouse gas reduction, removal of microconstituents from water, and water reclamation.
    Type: Grant
    Filed: June 28, 2010
    Date of Patent: October 9, 2012
    Assignee: HaloSource, Inc.
    Inventors: Everett J. Nichols, James R. Scott
  • Patent number: 8283533
    Abstract: The invention relates to the novel cotton variety designated 09R573B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R573B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R573B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R573B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: April 14, 2010
    Date of Patent: October 9, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Curtis Williams
  • Patent number: 8273968
    Abstract: The invention relates to the novel cotton variety designated 09R303B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R303B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R303B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R303B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: May 7, 2010
    Date of Patent: September 25, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Robert McGowen
  • Patent number: 8269075
    Abstract: The invention relates to the novel cotton variety designated 09R999B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R999B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R999B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R999B2R2 with itself or another cotton variety and plants produced by such method.
    Type: Grant
    Filed: April 14, 2010
    Date of Patent: September 18, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Curtis Williams
  • Publication number: 20120220036
    Abstract: Disclosed herein are media, kits, systems and methods for achieving micropropagation of bamboo on a commercially-relevant scale.
    Type: Application
    Filed: May 10, 2012
    Publication date: August 30, 2012
    Applicant: Boo-Shoot Gardens, LLC
    Inventors: Randall W. Burr, Jackie Heinricher
  • Patent number: 8252987
    Abstract: The invention relates to the novel cotton variety designated 09R643B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R643B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R643B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R643B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: April 14, 2010
    Date of Patent: August 28, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Curtis Williams
  • Patent number: 8247230
    Abstract: The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time.
    Type: Grant
    Filed: September 22, 2008
    Date of Patent: August 21, 2012
    Assignee: Unhwa Corporation
    Inventors: Mi Ok Jang, Eun Kyong Lee, Young Woo Jin
  • Patent number: 8234813
    Abstract: Systems and methods for hydroponically growing microorganisms within a self-contained air-supported structure, in which microorganisms are grown in an organic slurry, harvested, and processed to obtain and process and distribute molecules useful for biofuel or other purposes.
    Type: Grant
    Filed: April 17, 2009
    Date of Patent: August 7, 2012
    Assignee: Algepower LLC
    Inventors: Gail Busch, Jacques M. Dupont
  • Publication number: 20120184009
    Abstract: A method and device for growing plant, animal or stem cells in a continuous manner.
    Type: Application
    Filed: March 26, 2012
    Publication date: July 19, 2012
    Inventor: Eudes Francois Marie DE CRECY
  • Patent number: 8216840
    Abstract: In one aspect, a method is provided for producing stratified cotyledonary conifer somatic embryos. The method comprises (a) incubating a culture comprising immature conifer somatic embryos in a culture vessel comprising a development medium having an osmolality in the range of from 300 mM/Kg to 450 mM/Kg at a temperature of from 22° C. to 25° C. for a first incubation period sufficient in length for at least a portion of the embryos to reach anatomical maturity; and (b) subjecting the embryos in the culture vessel in accordance with step (a) to a temperature of from 0° C. to 10° C. for a second incubation period of at least one week to produce stratified cotyledonary somatic embryos.
    Type: Grant
    Filed: September 22, 2008
    Date of Patent: July 10, 2012
    Assignee: Weyerhauser NR Company
    Inventor: Amy M Jamruszka
  • Patent number: 8207418
    Abstract: The invention relates to the novel cotton variety designated 09R619B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R619B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R619B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R619B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: February 24, 2010
    Date of Patent: June 26, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Curtis Williams
  • Patent number: 8207419
    Abstract: The invention relates to the novel cotton variety designated 09R798B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R798B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R798B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R798B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: March 10, 2010
    Date of Patent: June 26, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Lawrence Burdett
  • Patent number: 8206983
    Abstract: The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.
    Type: Grant
    Filed: August 10, 2011
    Date of Patent: June 26, 2012
    Assignee: Dow AgroSciences, LLC.
    Inventors: P. Samuel Jayakumar, Jeffrey Beringer, Paul R. Schmitzer, Frank Burroughs, Robbi J. Garrison, William M. Ainley, Narasimha C. Samboju
  • Patent number: 8207416
    Abstract: The invention relates to the novel cotton variety designated 09R549B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R549B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R549B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R549B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: February 24, 2010
    Date of Patent: June 26, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Curtis Williams
  • Patent number: 8207417
    Abstract: The invention relates to the novel cotton variety designated 09R550B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R550B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R550B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R550B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: February 24, 2010
    Date of Patent: June 26, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Curtis Williams
  • Patent number: 8207420
    Abstract: The invention relates to the novel cotton variety designated 09R615B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R615B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R615B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R615B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: March 11, 2010
    Date of Patent: June 26, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Curtis Williams
  • Publication number: 20120156784
    Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
    Type: Application
    Filed: December 16, 2011
    Publication date: June 21, 2012
    Inventors: Whitney R. Adams, JR., Brian J. Martinell, Jyoti R. Rout, Edward J. Williams
  • Patent number: 8203051
    Abstract: The invention relates to the novel cotton variety designated 09R605B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R605B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R605B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R605B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: March 11, 2010
    Date of Patent: June 19, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Curtis Williams
  • Patent number: 8203050
    Abstract: The invention relates to the novel cotton variety designated 09R555B2R2. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 09R555B2R2. Also provided by the invention are tissue cultures of the cotton variety 09R555B2R2 and the plants regenerated therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 09R555B2R2 with itself or another cotton variety and plants produced by such methods.
    Type: Grant
    Filed: February 24, 2010
    Date of Patent: June 19, 2012
    Assignee: Monsanto Technology LLC
    Inventor: Curtis Williams
  • Publication number: 20120136046
    Abstract: The present invention relates to the use of fujimycin for the treatment of a disorder related to the chronological and/or replicative life-span of a cell, and to methods for increasing the replicative life span of a cell, said method comprising disrupting the function of a polynucleotide or gene encoding a polypeptide comprising SEQ ID No: 1, 3 or 5.
    Type: Application
    Filed: July 8, 2010
    Publication date: May 31, 2012
    Applicant: Chronos Therapeutics Limited
    Inventors: Elizabeth Jane Mellor, Michael Youdell, Jonathan Ayling, Alexandre Akoulitchev
  • Publication number: 20120128845
    Abstract: A system for cryoencapsulating a natural or manmade biological specimen which is capable of enabling the nucleation of at least one of benign polycrystalline or vitreous ice in the specimen via the introduction of particulate nuclei and cryoprotectant into the specimen including: (a) one or more specimen cooling modules or stations; (b) one or more specimen particulate or particulate-former introduction modules or stations; (c) one or more specimen cryoprotectant-introduction modules or stations; and (d) a system control unit and necessary system utilities, wherein the system is to implement a combined process of specimen cooling, specimen particulate introduction or formation, and specimen cryoprotectant introduction, and wherein the introduced particulate is to favorably enable the nucleation of one or both of encapsulating polycrystalline or vitreous ice resulting in ice formation with at least one of reduced ice damage or cryoprotectant toxic damage to the specimen during cryoencapsulation or during event
    Type: Application
    Filed: November 22, 2010
    Publication date: May 24, 2012
    Inventor: Carol Ann Tosaya
  • Publication number: 20120122224
    Abstract: Device for carrying out photochemical processes on a microscale and use of the device for photochemical reactions and culturing photosynthesizing cells and/or microorganisms.
    Type: Application
    Filed: May 5, 2010
    Publication date: May 17, 2012
    Applicant: EHRFELD MIKROTECHNIK BTS GMBH
    Inventors: Frank Schael, Karoly Nagy
  • Publication number: 20120107452
    Abstract: This invention relates to an aeration system comprising an aeration section, a degassing section and optionally an additional section. This invention also relates to a horizontal photobioreactor comprising the aeration system. This invention further relates to methods of using the photobioreactors.
    Type: Application
    Filed: April 16, 2010
    Publication date: May 3, 2012
    Inventors: Andreas Meiser, Lawrence A. Walmsley
  • Publication number: 20120077181
    Abstract: The cryopreservation of cells and tissue cultures for long term storage of cells, cell constructs or three-dimensional complex tissues assemblies is based on the use of a collagen cell carrier (CCC) having a specific composition and thickness and appropriate mechanical properties which are maintained after thawing. The collagen cell carrier provides a suitable support for the cryopreservation resulting in high survival rates after thawing and providing cells and tissue assemblies already adhered to a mechanically stable and biocompatible support. Frozen collagen carrier-cells assemblies, the frozen artificial cell constructs or three-dimensional complex tissue assemblies obtainable by the method and the use thereof after thawing are also disclosed.
    Type: Application
    Filed: February 18, 2010
    Publication date: March 29, 2012
    Inventors: Timo Schmidt, Lothar Just, Franz Maser, Holger Becker
  • Publication number: 20120058554
    Abstract: A cell culture polysaccharide microcarrier includes (1) a cross-linked polysaccharide microcarrier base having a neutral or negative charge at pH 7, and (ii) a polypeptide conjugated to the base. The polypeptide may contain a cell adhesive sequence, such as RGD. Cells cultured with such microcarriers exhibit peptide-specific binding to the microcarriers.
    Type: Application
    Filed: July 28, 2010
    Publication date: March 8, 2012
    Inventors: Sophie Deshayes, David Henry, Martial Hervy