By Acylation Of The Substituent In 6-position Patents (Class 435/45)
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Patent number: 8663561Abstract: An automated apparatus and method for analyzing liquid samples by forming discrete sample aliquots (boluses) in an elongated conduit which contains a hydrophobic carrier liquid. Aliquots may be analyzed by adding at least one reagent to the sample aliquot that reacts selectively with an analyte contained therein. The reaction product, which is selective for the analyte of interest and proportional to its concentration, is measured with an appropriate detector. Intrinsic sample properties of the sample may also be measured without the need for adding chemical reagents. The invention enables simple and accurate testing of samples using time honored wet-chemical analysis methods in microliter volume regimes while producing remarkably small volumes of waste.Type: GrantFiled: February 19, 2010Date of Patent: March 4, 2014Inventor: Charles J. Patton
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Patent number: 8497088Abstract: The present invention describes a process for the synthesis of a semi-synthetic ?-lactam compound from a nucleus and a side chain selected from the group consisting of D-phenylglycine and D-dihydro-phenylglycine in the form of a side chain ester and an enzyme catalyzing the coupling of the side chain ester to the nucleus characterized in that the side chain ester is not isolated as a solid intermediate.Type: GrantFiled: March 10, 2008Date of Patent: July 30, 2013Assignee: DSM Sinochem Pharmaceuticals Netherlands B.V.Inventors: Thomas Van Der Does, Harold Monro Moody, Theodorus Johannes Godfried Maria Van Dooren
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Patent number: 8409447Abstract: A separation chip for separating an insoluble component from a suspension using centrifugal force by rotation, includes a suspension holding tank, a separation liquid holding tank, and an insoluble component holding tank arranged in order from an inner circumferential side during rotation, wherein the suspension holding tank and the insoluble component holding tank are connected to each other, the insoluble component holding tank and the separation liquid holding tank are connected to each other by a narrow portion, and in the insoluble component holding tank, a connecting portion with the suspension holding tank is positioned further toward an outer circumferential side than the narrow portion.Type: GrantFiled: January 27, 2009Date of Patent: April 2, 2013Assignee: Toray Industries, Inc.Inventors: Kentaro Ishii, Masashi Higasa, Shingo Hiramatsu
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Publication number: 20100190206Abstract: The present invention discloses isolation of Penicillin Acylase (PA) from Achromobacter sp CCM 4824 expressed in recombinant strain E. coli BL21 CCM 7394 bearing the recombinant plasmid pKXIP1 and processing of PA into biocatalyst useful for the industrial synthesis of antibiotics. More particularly the invention discloses a synthesis of semi-synthetic ?-lactam antibiotics in the reaction mixture consisting of activated acyl-donor (D-p-hydroxyphenylglycine methyl ester or amide for Amoxicillin and Cefadroxil; D-phenylglycine methyl ester or amide for Ampicillin and Cephalexin) and nucleophile (6-APA or 7-ADCA) catalyzed by PA obtained from recombinant E. coli BL21 CCM 7394 as the biocatalyst.Type: ApplicationFiled: July 27, 2007Publication date: July 29, 2010Inventors: Anupama Datla, Vyasarayani Williams Rajasekar, Pavel Kyslik, Stanislav Becka, Ashar Trupti Krishnakant, Zambre Sujata Yogesh, Kumar Nikunj
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Patent number: 7264949Abstract: The promoter region associated with the Yarrowia lipolytica glycerol-3-phosphate O-acyltransferase (gpat) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be suitable to drive high-level expression of genes involved in the production of ?-3 and ?-6 fatty acids.Type: GrantFiled: September 13, 2005Date of Patent: September 4, 2007Assignee: E.I. du Pont de Nemours and CompanyInventors: Zhixiong Xue, Quinn Qun Zhu
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Patent number: 7256018Abstract: Provided are a microorganism capable of producing L-threonine and having an inactivated tyrR gene, a method of producing the same and a method of producing L-threonine using the microorganism. The microorganism can be used to produce L-threonine in high yield.Type: GrantFiled: February 4, 2005Date of Patent: August 14, 2007Assignee: CJ CorporationInventors: Young Hoon Park, Byoung Choon Lee, Jae Yong Park, Kwang Myung Cho, Yong Uk Shin
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Patent number: 7241594Abstract: Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.Type: GrantFiled: September 16, 2005Date of Patent: July 10, 2007Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Hyohak Song, Yu Sin Jang, Sung Won Lim
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Patent number: 7229794Abstract: Provided are a microorganism capable of producing L-threonine and having an inactivated galR gene, a method of producing the same and a method of producing L-threonine using the microorganism. The microorganism can be used to produce L-threonine in high yield.Type: GrantFiled: February 4, 2005Date of Patent: June 12, 2007Assignee: CJ CorporationInventors: Young Hoon Park, Byoung Choon Lee, Kwang Myung Cho, Dae Cheol Kim, Yong Uk Shin, Jin Ho Lee
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Patent number: 7223567Abstract: The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.Type: GrantFiled: August 29, 2005Date of Patent: May 29, 2007Assignee: Rice UniversityInventors: San Ka-Yiu, George N. Bennett, Ailen Sanchez
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Patent number: 7153676Abstract: Transacylase enzymes and the use of such enzymes to produce paclitaxel, related taxoids, as well as intermediates in the paclitaxel biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the transacylase enzymes.Type: GrantFiled: September 29, 2000Date of Patent: December 26, 2006Assignee: Washington State University Research FoundationInventors: Rodney B. Croteau, Kevin D. Walker, Anne Schoendorf, Mark R. Wildung
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Patent number: 6995045Abstract: The thin film transistor has a non-transparent structure besides and insulated with the gate. Hence, the light transmitted from the substrate is blocked and the light current induced in the thin film transistor is negligible. The method uses a mask with a slit pattern to form a non-uniform photoresist. Hence, the mask could be used to pattern two conductor layers for forming source/drain/channel.Type: GrantFiled: March 5, 2003Date of Patent: February 7, 2006Assignee: Chunghwa Picture Tubes, Ltd.Inventors: Hung-Jen Chu, Nei-Jen Hsiao, Hui-Chung Shen, Meng-Chi Liou
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Patent number: 6518039Abstract: The present invention discloses a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.Type: GrantFiled: May 17, 1999Date of Patent: February 11, 2003Assignee: DSM N.V.Inventors: Maarten Nieboer, Erik De Vroom, Johannis Lugtenburg, Dirk Schipper, Adrianus Wilhelmus Hermanus Vollebregt, Roelof Ary Lans Bovenberg
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Patent number: 6503727Abstract: The invention relates to a process for the preparation of an antibiotic, in particular cefalexin, ampicillin, amoxicillin, cefaclor, cefradin, cefadroxil, cefotaxim and the like, wherein a beta-lactam core is acylated, the antibiotic is recovered from the reaction mixture, the remaining mother liquor is subjected to a hydrolysis reaction in which the antibiotic present in the mother liquor is decomposed into its initial compounds, in particular the beta-lactam core, and the acylation agent is hydrolized. The beta-lactam core can then be recovered virtually quantitatively or recycled to the acylation reaction. This is because it has been found that the solubility of the beta-lactam core is unexpectedly high at relatively high concentrations of acylation agent and antibiotic.Type: GrantFiled: June 5, 1998Date of Patent: January 7, 2003Assignee: Gist-Brocades, B.V.Inventors: Wilhelmus H. J. Boesten, Wilhelmus J. J. Van Den Tweel, Rocus M. Dekkers
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Patent number: 6455284Abstract: Metabolic engineering is used to increase the carbon flow toward oxaloacetate to enhance production of bulk biochemicals, such as lysine and succinate, in bacterial fermentations. Carbon flow is redirected by genetically engineering the cells to overexpress the enzyme pyruvate carboxylase.Type: GrantFiled: October 13, 1999Date of Patent: September 24, 2002Assignee: The University of Georgia Research Foundation, Inc.Inventors: Ravi R. Gokarn, Mark A. Eiteman, Elliot Altman
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Patent number: 6420178Abstract: A liquid chemical stripping or cleaning solution is selected by combinatorial high throughput screening. A high throughput screening well array assembly includes (A) a metal substrate and (B) a mask that defines an array of wells on the substrate. A combinatorial high throughput screening system includes (A) a metal substrate and (B) a mask that defines an array of wells on the substrate and a reaction vessel to receive the well array assembly.Type: GrantFiled: September 20, 2000Date of Patent: July 16, 2002Assignee: General Electric CompanyInventors: John Robert LaGraff, Xiao-Dong Sun, James Anthony Ruud, James Claude Carnahan
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Patent number: 6403356Abstract: New mutant penicillin G acylases preferably from E. coli are provided, exhibiting altered enzymatic activity. These penicillin G acylases are obtained by expression of a gene encoding said penicillin G acylase having an amino acid sequence which differs at least in one amino acid from the wild-type penicillin G acylase.Type: GrantFiled: October 31, 1997Date of Patent: June 11, 2002Assignee: Bristol-Myers Squibb Co.Inventors: Li You, John James Usher, Brenda Joyce White, Jiri Novotny
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Publication number: 20020006642Abstract: The invention relates to a method for preparing a &bgr;-lactam antibiotic, wherein an N-substituted &bgr;-lactam, having general formula (I), wherein R0 is hydrogen or C1-3 alkoxy; Y is CH2, oxygen, sulfur, or an oxidized form of sulfur; Z is (a), (b), (c) or (d), wherein R1 is hydrogen, hydroxy, halogen, C1-3 alkoxy, optionally substituted, optionally containing one or more heteroatoms, saturated or unsaturated, branched or straight C1-5 alkyl, preferably methyl, optionally substituted, optionally containing one or more heteroatoms C5-8 cycloalkyl, optionally substituted aryl or heteroaryl, or optionally substituted benzyl; and X is (CH2)m-A-(CH2)n, wherein m and n are the same or different and are chosen from the group of integers 0, 1, 2, 3 or 4 and A is CH═CH, C═C, CHB, C═O, optionally substituted nitrogen, oxygen, sulfur or an optionally oxidized form of sulfur, and B is hydrogen, halogen, hydroxy, C1-3 alkoxy, or optionally substituted methyl, or a salt thereof, is contacted with at leastType: ApplicationFiled: May 3, 1999Publication date: January 17, 2002Inventors: THOMAS VAN DER DOES, JAGDISH CHANDER KAPUR, ERIK DE VROOM
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Patent number: 6218138Abstract: Beta-lactam antibiotics are synthesized by reacting an amino-beta-lactam component with a corresponding amino-group-containing acylating side-chain component in the presence of penicillin amidase from E. coli covalently immobilized on support particles. The resulting beta-lactam antibiotic product is solubilized by adding an acid such as sulfuric acid to lower the pH to 1.0 at a temperature in the range of 0° C. to +5° C. The immobilized penicillin amidase is substantially inactivated by the acid. After separating the beta-lactam antibiotic product, the immobilized penicillin amidase is substantially reactivated for reuse in antibiotic synthesis by treatment with a buffer having about a neutral pH. Antibiotics that can be produced include ampicillin, amoxicillin, cephalexin, cefaclor and cefadroxil.Type: GrantFiled: May 26, 1999Date of Patent: April 17, 2001Assignee: Unifar Kimya Sanayi ve Ticaret A.S.Inventors: Ferhat Ilhan, Dieter Kraemer
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Patent number: 6060268Abstract: Penicillin G acylase is immobilized by covalent bonding to a crosslinked mixture of a gelled gelling agent such as gelatin and a polymer containing free amino groups such as alginate amine, chitosan or polyethylene imine. The immobilized penicillin G acylase provides a higher synthesis/hydrolysis ratio as compared to immobilizing with other carriers when producing .beta.-lactam derivatives by a condensing reaction of an amino .beta.-lactam with an acylating agent. The acylating agent may be a derivative of D-phenylglycine, a derivative of D-p-hydroxyphenylglycine or a derivative of D-2,5-dihydro-phenylglycine. Examples of .beta.-lactam derivatives that can be produced are amoxycillin, ampicillin, cephaclor, cephadroxil, cephprozil, cephalexin and cephradine.Type: GrantFiled: January 15, 1998Date of Patent: May 9, 2000Assignee: Gist-Brocades B.V.Inventor: Erik De Vroom
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Patent number: 6048708Abstract: An improvement for enzymatically syhnthesizing a .beta.-lactam compound is presented. The improvement comprises catalyzing the acylation of an amino .beta.-lactam with an acylating agent for at least 5 hours with an amidase or acylase, wherein the concentration of each reactant is constantly higher than 70% of the lowest value of the saturated concentration of both reactants.Type: GrantFiled: February 19, 1997Date of Patent: April 11, 2000Assignee: Gist-Brocades B.V.Inventors: Kim Clausen, Rocus M. Dekkers
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Patent number: 6033823Abstract: New mutant .beta.-lactam Penicillin G acylases are provided, exhibiting altered substrate specificities. These Penicillin G acylases are obtained by expression of a gene encoding said Penicillin G acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type Penicillin G acylase.Type: GrantFiled: April 5, 1999Date of Patent: March 7, 2000Inventors: Jan M. Van Der Laan, Adriana M. Riemens, Wilhelmus J. Quax
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Patent number: 5935799Abstract: A semi-quantitative method for determining the concentration of viable coliforms or E. coli in a liquid is provided. The sample to be tested is mixed with an indicator reagent which includes a specific indicator compound that undergoes a change that is detectable by spectrophotometric or visual methods upon cleavage by a beta galactosidase enzyme found in coliforms or a beta glucuronidase enzyme unique to E. coli.Type: GrantFiled: December 10, 1997Date of Patent: August 10, 1999Assignee: George Mason UniversityInventor: Jenefir D. Isbister
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Patent number: 5916762Abstract: Process for the recovery of ampicillin from a mixture containing ampicillin and 6-aminopenicillic acid (6-APA), in which a mixture of ampicillin and 6-APA, with a pH higher than 7, which apart from any solid ampicillin being present is homogeneous at a pH between 7 and 8.5, is subjected to a pH lowering till a pH lower than 8.2 is reached, and the solid substance present is recovered. The process is in particular suitable to be applied to the reaction mixture which is obtained after the enzymatic acylation reaction of 6-APA with a phenylglycidine derivative as acylation agent. Pure ampicillin can thus be recovered in a simple way.Type: GrantFiled: September 30, 1997Date of Patent: June 29, 1999Assignee: Chemferm V.O.F.Inventors: Wilhelmus H. J. Boesten, Harold M. Moody, Eric C. Roos
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Patent number: 5891703Abstract: New mutant .beta.-lactam Penicillin G acylases are provided, exhibiting altered substrate specificities. These Penicillin G acylases are obtained by expression of a gene encoding for said Penicillin G acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type Penicillin G acylase.Type: GrantFiled: April 23, 1997Date of Patent: April 6, 1999Assignee: Gist-HrocadesInventors: Jan M. Van Der Laan, Adriana M. Riemens, Wilhelmus J. Quax
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Patent number: 5780260Abstract: An enzyme selected from penicillin-G amidase, glutaryl-7-ACA acylase and D-amino acid oxidase is immobilized by covalent bonding on an aminofunctional organosiloxane polymer carrier to provide an immobilized enzyme having a specific volume activity of at least 100 U/g wet carrier. Preferably, the carrier has an average diameter or 0.01 to 3 mm and is essentially spherical. Covalent bonding is accomplished by activating amino groups on the carrier with a dialdehyde and reacting the activated groups with reactive groups on the enzyme. An amount of enzyme is immobilized to provide a weight ratio of enzyme to carrier of 1 to 300 mg protein per g wet carrier.Type: GrantFiled: May 30, 1996Date of Patent: July 14, 1998Assignee: Boehringer Mannheim GmbHInventors: Frank Wedekind, Adelheid Daser, Wilhelm Tischer
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Patent number: 5753458Abstract: A method for providing a semisynthetic .beta.-lactam antibiotic by enzyme catalyzed acylation of the parent .beta.-lactam with an activated derivative of the side chain acid wherein a modulator, which consists of one or more compounds different from the reactants and the reaction product and which suppresses the hydrolysis of the activated derivative of the side chain acid and the desired product more than it suppresses the synthesis of the desired product, is added to the reaction mixture, at the beginning of the reaction process, in a concentration from about 0.2 to 100.times.10.sup.3 .mu.m.Type: GrantFiled: February 7, 1996Date of Patent: May 19, 1998Assignee: Gist-Brocades B.V.Inventors: Kim Clausen, Annette Nielsen, Niels Petersen, Alexander Nikolov
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Patent number: 5525483Abstract: .beta.-Lactam derivatives are synthesized by an enzymatic reaction of the parent amino .beta.-lactam with the corresponding acylating agent, the concentration of the acylating agent plus the concentration of .beta.-lactam derivative in the reaction mixture being above about 400 mM.Type: GrantFiled: January 18, 1994Date of Patent: June 11, 1996Assignee: Gist Brocades B.V.Inventors: Svend G. Kaasgaard, Ulla Veitland
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Patent number: 5457032Abstract: New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.Type: GrantFiled: May 9, 1991Date of Patent: October 10, 1995Assignee: Gist-brocades NVInventors: Wilhelmus J. Quax, Onno Misset, Jan M. Van Der Laan, Herman B. M. Lenting
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Patent number: 5268271Abstract: Synthesis of semi-synthetic monobactamic or .beta.-Lactamic antibiotics by using derivatives stabilized by various methods of penicillin G acylase from various microbial sources according to a thermodynamically controlled strategy in monophase water/cosolvent organic apolar systems, wherein the concentration of the cosolvent varies between 30% and 90%, the temperature between -10.degree. C. and 50.degree. C., the pH between 4.5 and 8.5, with concentrations of the antibiotic nucleus between 0.5 an 875 mM and acyl donor between 0.2 mM and 1M, with a relationship antibiotic ring/activated or free acyl donor, using a buffer between 0 and 1M. Application to the pharmaceutical industry.Type: GrantFiled: August 2, 1991Date of Patent: December 7, 1993Assignee: Cosejo Superior de Invetigaciones CertificasInventors: Jose M. Guisan Seijas, Roberto Fernandez Lafuente, Gregorio Alvaro Campos, Rosa M. Blanco Martin, Cristina Molina Rosell
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Patent number: 5215891Abstract: A process for preparing a compound of formula I: ##STR1## wherein R.sub.1 represents a hydroxy protecting group and R.sub.2 represents a carboxy protecting group, which comprises:hydrolyzing a compound of formula II ##STR2## wherein R.sub.1 and R.sub.2 are as defined above and R represents an alkyl, alkenyl or phenylalkyl group having from 1 to 18 carbon atoms, in the presence of an enzyme capable of selectively hydrolyzing the ester group of the 2-substituent thereof.Type: GrantFiled: March 9, 1992Date of Patent: June 1, 1993Assignee: Farmitalia Carlo ErbaInventors: Maria Altamura, Pietro Cesti, Franco Francalanci, Marcello Marchi, Marco Foa', Stefano Cambiaghi, Franco Dallatomasina
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Patent number: 5080988Abstract: The operating latitude of the tri-level xerographic process is improved by replacing the standard DC bias that is applied to one or both of the developer housings in conventional tri-level imaging with a chopped DC (CDC) developer bias. Chopped DC biasing is the alternate application of two discrete bias voltages to a developer stucture in a periodic fashion at a given frequency, with the period of each cycle divided up between the two bias levels at a duty cycle of from 5%-10% or 90%-95% depending upon which of the two developer structures is being biased. In the case of the DAD developer structure the duty cycle of higher of the two biases is 5%-10% and in case of a CAD developer structure the duty cycle of higher of the two biases is 90%-95%.Type: GrantFiled: November 22, 1989Date of Patent: January 14, 1992Assignee: Xerox CorporationInventors: Richard P. Germain, James E. Williams
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Patent number: 5079146Abstract: The present invention relates to a new method of protection of the carboxy group in the chemistry of the compounds of .beta.-lactam type. According to such a method, the carboxy group is protected by being transformed into its corresponding phenyl-acetoxy-methyl ester, which is then removed by an enzymatic route by means of penicillinacylase.In particular, if the .beta.-lactam compound also bears a phenyl-acetamidic substitutent, this latter is simultaneously hydrolysed during the same step of removal of the carboxy function protecting group.Type: GrantFiled: April 4, 1989Date of Patent: January 7, 1992Assignee: Sclavo S.p.A.Inventors: Claudio Fuganti, Eva Baldaro, Daniela Faiardi, Ameriga Lazzarini
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Patent number: 5008188Abstract: Process for producing S-adenosyl-L-homocysteine, which comprises reacting adenosine with homocysteine by contacting them in an aqueous medium in the presence of cells or treated cells of a microorganism of a specified genus having the ability to synthesize S-adenosyl-L-homocysteine from adenosine and homocysteine, and collecting the S-adenosyl-L-homocysteine synthesized.Type: GrantFiled: November 4, 1987Date of Patent: April 16, 1991Assignee: Nippon Zeon Co., Ltd.Inventors: Hideaki Yamada, Yoshiki Tani, Sakayu Shimizu, Shozo Shiozaki
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Patent number: 4945159Abstract: A method for the formation of intramolecular amide bonds by the action of cyanogen, under mild reaction conditions, in the preparation of cyclic amides, including lactams, in biologically active compounds. A compound containing at least one carboxylic acid group and at least one primary or secondary amino group is reacted with cyanogen to form an intramolecular amide bond. The method has utility in the synthesis of B-lactam antibiotics, such as penicillins, cephalosporins, and their derivatives, in enzyme modification, in cyclization of peptides, and in covalent cross-linking of proteins.Type: GrantFiled: December 2, 1986Date of Patent: July 31, 1990Assignee: University of CincinnatiInventor: Richard A. Day
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Patent number: 4340672Abstract: Penicillins and cephalosporins can be synthesized by the action of an acylase on a penicillin or cephalosporin nucleus amine as substrate and an ester (I) of the following formula as the acyl source: ##STR1## (wherein RCO is an acyl group in penicillin or cephalosporin side chains;X is a hydrogen atom, lower alkyl group or hydroxy-lower alkyl group;Y is a hydrogen atom or a lower alkyl group; and n is a positive integer).The novel acyl source (I) is also disclosed.Type: GrantFiled: September 19, 1980Date of Patent: July 20, 1982Assignee: Shionogi & Co., Ltd.Inventors: Eiji Kondo, Takashi Mitsugi, Tamio Fujiwara, Ryonosuke Muneyuki
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Patent number: 4250258Abstract: Penicillin V is prepared by culturing a penicillin-producing microorganism in a medium containing suitable sources of nitrogen, carbon and energy, and inorganic salts. One or more phenoxyalkanes of the formula ##STR1## wherein n is an integer from 6 to 13 are included within the medium as the sidechain precursor.Type: GrantFiled: December 20, 1979Date of Patent: February 10, 1981Assignee: E. R. Squibb & Sons, Inc.Inventors: Laszlo J. Szarka, Robert W. Eltz
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Patent number: 4167446Abstract: A water-soluble non-colloidal penicillinacylase preparation containing penicillinacylase covalently bound to a water-soluble carrier is produced by reacting penicillinacylase with an activated derivative of a water soluble polysaccharide in aqueous solution. The water-soluble penicillinacylase preparation efficiently hydrolyzes penicillin and permits recovery of 6-aminopenicillanic acid efficiently in extremely high purity.Type: GrantFiled: October 30, 1975Date of Patent: September 11, 1979Assignee: Bayer AktiengesellschaftInventor: Fritz Huper