Abstract: The present invention refers to gene expression cassettes encoding enzymes involved in the metabolic pathway for the biosynthesis of hemicelluloses, cellulose and/or uronic acids, and to a method for genetic transformation of plant cells through the introduction of one or more gene expression cassettes from the present invention, and the overexpression and repression of these genes in plants. More particularly, the present invention refers to a method for introducing expression cassettes in plants. Additionally, the present invention refers to genetically modified plants. The present invention also refers to the attainment of the genetically modified plant, its derived plants and seeds, as well as the wood, paper and cellulose from this plant.

Abstract: The present invention provides methods for enhancing plant transformation. One such method entails making an excision in a selected seedling at the point where two cotyledons meet, and then vortexing the cut seedling in a solution comprising a transforming bacterium such as Agrobacterium.

Abstract: The present invention provides means and methods of transforming plant cells, seeds, tissues or whole plants in order to yield transformants capable of expressing all enzymes of the carotenoid biosynthesis pathway that are essential for the targeted host plant to accumulate carotenes and/or xanthophylls of interest. The present invention also provides DNA molecules designed to be suitable for carrying out the method of the invention, and plasmids or vector systems comprising said molecules. Furthermore, the present invention provides transgenic plant cells, seeds, tissues and whole plants that display an improved nutritional quality and contain such DNA molecules and/or that have been generated by use of the methods of the present invention.

Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

Abstract: The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced to Acacia mangium via Agrobacterium infection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.

Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.

Abstract: The present disclosure provides methods for obtaining the targeted integration of a DNA molecule into the genome of a host cell using a recombinase. The methods disclosed herein can be used with a variety of host cells, including, for example, dicotyledonous and monocotyledonous plant cells. The present disclosure provides a method for effecting site-specific recombination of DNA within a plant cell, comprising: introducing into the plant cell a target nucleotide sequence comprising a first Int recognition site; introducing into the plant cell a donor nucleotide sequence comprising a second Int recognition site; and introducing into the plant cell an integrase or integrase complex.

Abstract: The present invention relates to a binary vector which is distinguished by the combination of a variety of elements, demonstrates particularly high compatibility from the cloning aspect and which, besides the T-DNA flanked by the right and left border, contains an additional sequence which makes possible highly efficient and correct transfer of the T-DNA.

Abstract: Development of an efficient and cost-effective doubled haploid production system and genetic transformation system are the prerequisite to initiate haploid breeding and genetic modification in flax respectively. Pre-culturing anthers on a high osmotic, high auxin and high mineral salt concentration for a period of time before transfer to a low osmotic, low auxin and low salt concentration significantly increased the overall efficiency of regeneration or anther efficiency than directly culturing anthers on a low osmotic, low auxin and low salt concentration medium. This culture procedure also dramatically reduced the frequency of shoot regeneration from somatic cells in anther culture. Using this procedure, a highly efficient anther culture-derived callus based transformation system was developed.

Abstract: The subject invention pertains to materials and methods for producing plants that are resistant to infection by geminiviruses and other related viruses. Methods of the invention comprise transforming a plant with a polynucleotide wherein when the polynucleotide is expressed in the plant, the transformed plant exhibits resistance to plant viral infections. Exemplified herein is the use of a polynucleotide encoding a Rep protein derived from tomato mottle geminivirus. The methods of the invention can be used to provide resistance to viral infection in plants such as tomato and tobacco. The present invention also concerns transformed and transgenic plants in plant tissue that express a polynucleotide encoding a plant virus Rep protein, or a fragment or variant thereof.

Abstract: A method of concurrently introducing multiple genes to plants and trees is provided. The method includes an Agrobacterium-mediated gene delivery system by which multiple genes together with a single selectable marker gene are simultaneously transferred and inserted into the genome of plants, including trees.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a sucrose transport protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sucrose transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sucrose transport protein in a transformed host cell.

Abstract: A transformation method comprising inoculation and co-cultivation of a target tissue, from a target plant, with Agrobacterium, at a time when the target tissue is in its natural plant environment, followed by generation of a transgenic plant via dedifferentiation and regeneration of the target tissue.

Abstract: Methods and compositions to increase Agrobacterium transformation efficiency (frequencies) in both dicot and monocot host plants include adding histones to the host plant at most transiently, and using histones and L-cysteine at certain stages in monocot transformation.

Abstract: A synthetic polynucleotide encoding human lactoferrin, modified with respect to the natural gene so as to maximize its expression in vegetals, on the basis of the preferential use of the codons is described. Moreover, the vectors containing such sequence, that having regulation elements activated in a controlled way determine its tissue- and stage-specific expression are further described. The vegetal cells and the plants transformed with the afore mentioned vectors, as well as the production processes of functional foods, vegetal milks, and human lactoferrin, utilizing them are also described.

Abstract: Adding at least one gene involved in plant host cell T-DNA integration enhances transformation by Agrobacterium. The histone H2A gene encoded by the Arabidopsis RAT5 gene increases transformation frequencies of plants, most likely by causing overexpression of a product needed for T-DNA integration. Agrobacterium tumefaciens genetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, designated the T-DNA, to the plant, and integrating the T-DNA into the plant genome. However, not all plants are transformable by Agrobacterium and transformation frequencies may be too low to be useful. Little is known about the T-DNA integration process, and no plant genes involved in integration have been identified prior to the present invention.

Abstract: Nucleic acid sequence capable of regulating transcription during embryogenesis in plants is provided. This sequence may be used in transgenic plants to promote high levels of expression of foreign and endogenous genes in developing seeds to affect seed lipid metabolism, protein or carbohydrate composition and accumulation, or seed development. In addition, these sequences may be useful for the production of modified seed containing novel recombinant proteins which have pharmaceutical, industrial or nutritional value, or novel products like plastics.

Abstract: The present invention relates to an improved transformation and regeneration system for wheat. In particular, the invention relates to enhancements in the glyphosate selection system. The transformation method is efficient and reliable for production of fertile plants with improved agronomic qualities.

Abstract: Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal lipase gene products that are useful in the practice of this invention are also provided. The compositions and methods of the invention may be used in a variety of agricultural systems for controlling pests, including propagating lineages of insect-resistant crops and targeting expression of these insecticidal lipases to plant organs that are particularly susceptible to infestation, such as roots and leaves.

Abstract: A method for obtaining a transgenic plant that over-expresses a soluble isoform AGPPase enzyme. The method includes a step of transforming a plant with a vector comprising a polynucleotide of SEQ ID NO: 7 linked to a promoter that promotes expression of the polynucleotide in the plant whereby to form the transgenic plant. The transgenic plant has a reduced starch content and a higher resistance to salinity than the plant before the transforming step.

Abstract: The present invention is directed to promoters of flax conlinin and ?-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.

Abstract: The present invention relates to methods for the transformation and regeneration of transformed embryogenic tissue of coniferous plants. In particular, the invention relates to improved methods for transforming embryogenic tissue of coniferous plants and for regenerating transformed embryogenic tissue of coniferous plants. The invention is well suited to the transformation and regeneration of transformed embryogenic tissue of plants of the subgenus Pinus of pines and hybrids thereof.

Abstract: The present invention relates to an efficient and cost effective method of preventing growth of genetic transformant bacteria Agrobacterium tumefaciens after transformation in plants by using tea leaf extract as a bactericide, wherein said method leads to elimination of common problem of polyphenol oxidation during transformation and thereby helps maintain regeneration potential in explants and also helps in increased transformation efficacy

Abstract: Adding at least one gene involved in plant host cell T-DNA integration enhances transformation by Agrobacterium. The histone H2A gene encoded by the Arabidopsis RAT5 gene increases transformation frequencies of plants, most likely by causing overexpression of a product needed for T-DNA integration. Agrobacterium tumefaciens genetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, designated the T-DNA, to the plant, and integrating the T-DNA into the plant genome. However, not all plants are transformable by Agrobacterium and transformation frequencies may be too low to be useful. Little is known about the T-DNA integration process, and no plant genes involved in integration have been identified prior to the present invention.

Abstract: The present invention relates to novel methods for the transformation of crop species, novel methods for the selection and identification of transformed plant cells and novel methods for recovery of regenerated whole plants. The method also relates to the development of plants with novel traits and plants that contain novel recombinant DNA constructs.

Abstract: The present invention relates to a method for transforming Allium species with a heterologous gene using Agrobacterium.

Abstract: The invention relates to a method for transforming a monocotyledonous plant. The time required from transformation to regeneration of a plant is shorter using the inventive method so that the frequency of emergence of mutants is smaller than the conventional methods. The inventive method may be generally applied even to the plants for which a regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy.

Abstract: A method of obtaining transgenic turfgrass plants by an Agrobacterium-mediated transformation protocol is disclosed. The protocol makes use of a modified Agrobacterium vector system in which selectable marker genes and other genes of interest are operably linked to strong promoters from monocotyledenous plants, such as actin and ubiquitin promoters, that function efficiently in turfgrass cells. Transgenic turfgrass plants of several species, produced by the Agrobacterium-mediated transformation method, are also disclosed.

Abstract: The present invention relates to a rapid transformation and regeneration system for plants. In particular, the invention relates to a plant tissue preparation system. The transformation method is efficient and reliable for production of fertile plants with improved agronomic qualities.

Abstract: Disclosed are processes for producing stably transformed fertile wheat a system of transforming wheat via Agrobacterium. This invention provides methods transforming a variety of explants, such as freshly isolated or pre-cultured immature embryos, embryogenic callus and suspension cells. Also disclosed are methods for recovering transgenic plants after transformation within a short period of time, if the explants are regenerable at the time of transformation. Thus the frequency of somaclonal variation associated with prolonged in vitro culture period is significantly reduced. The transformation frequency using this system is comparable to or better than published methods using other systems, such as microprojectile bombardment.

Abstract: This invention provides materials and methods to manipulate the plant genome at the level of single plant cells in culture resulting in the ability to assign metabolic functionality to plant genes involved in the production of biologically active molecules and to create a means of product discovery based on the biosynthetic capacity of plants. The materials to create an activation mutagenesis include incorporation of enhancer sequences from a plant viral promoter at random places in the plant genome via Agrobacterium mediated DNA transfer (T-DNA). The usefulness is that genes in the immediate vicinity of the incorporation were activated which allows for immediate screening of the mutagenized plant cells. Additionally, the usefulness includes relevant areas of the genome were flanked by the inserted T-DNA which allows recovery of this area by standard molecular biology techniques.

Abstract: The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited minichromosomes which can serve as vectors for the construction of transgenic plant and animal cells. In addition, information on the structure and function of these regions will prove valuable in isolating additional centromeric and centromere related genetic elements and polypeptides from other species.

Abstract: A canola line designated 43A56, plants and seeds of the 43A56 canola line, methods for producing a canola plant produced by crossing the 43A56 line with itself or with another canola plant, and hybrid canola seeds and plants produced by crossing the 43A56 line with another canola line or plant are provided.

Abstract: Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as Agaricus bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.

Abstract: The present invention relates to a method for conferring tolerance to salt stress and drought stress in a monocot plant including transforming the monocot plant with an expression cassette comprising at least one ABRC unit, a minimal promoter, and a DNA molecule that increases tolerance to salt stress and drought stress in plants, wherein the at least one ABRC unit, the minimal promoter, and a DNA molecule are operably linked together to permit expression of the DNA molecule. The present invention also relates to a transgenic monocot plant transformed with a DNA molecule that increases tolerance to salt stress and drought stress operably linked to at least one ABRC unit and a minimal promoter.

Abstract: A novel garden bean cultivar, designated ‘210104’, is disclosed. The invention relates to the seeds of garden bean cultivar ‘210104’, to the plants of garden bean line ‘210104’ and to methods for producing a bean plant by crossing the cultivar ‘210104’ with itself or another bean line. The invention further relates to methods for producing a bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar ‘210104’.

Abstract: A novel potato cultivar of the genus and species Solanum tuberosum, designated FL1900, is disclosed. The invention relates to the tubers of potato variety FL1900, to the plants of potato variety FL1900, to the seeds of potato variety and to methods for producing hybrid potato variety. The invention further relates to potato variety tubers, seeds and plants produced by crossing the potato variety FL1900 with another potato plant, and to Single Gene Converted plants.

Abstract: The present invention relates to isolated proteins or polypeptides of grapevine leafroll virus (type 2). The encoding DNA molecules either alone in isolated form or in an expression system, a host cell, or a transgenic grape plant are also disclosed. Other aspects of the present invention relates to a method of imparting grapevine leafroll resistance, to grape and tobacco plants by transforming them with the DNA molecules of the present invention, a method of imparting beet yellows virus resistance to a beet plant, a method of imparting tristeza virus resistance to a citrus plant, and a method of detecting the presence of a grapevine leafroll virus, such as GRLaV-2, in a sample.

Abstract: Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.

Abstract: The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced to Acacia mangium via Agrobacterium infection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.

Abstract: The invention involves decreasing the intracellular availability of trehalose-6-phosphate by plant cell transformation with a gene encoding trehalose-6-phosphate phosphatase from E. coli. Phenotypic effects of plant transformation with this gene include stimulation of glycolysis, cell or tissue growth, and metabolism; and inhibition of photosynthesis and bolting.

Abstract: The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

Abstract: Transgenic plants which express cellulose-degrading enzymes, methods to make the transgenic plants, and methods to use the cellulose-degrading enzymes produced by the transgenic plants are disclosed.

Abstract: A method for embryogenesis and a method for plant regeneration are disclosed. Microspore-containing plant segment from donor plants are harvested and incubated under pre-treatment conditions to maintain microspore at a uninucleate cell cycle G1 phase. Pre-treatment conditions comprise cold water or an aqueous solution of about 0.2-about 1.0 mol/liter sugar alcohol, for example mannitol. Microspores are isolated from the plant segment and embryogenesis of microspores is induced in induction medium, thereby producing embryos. Green plants may be regenerated from the embryos produced. Arabinogalactan protein, auxin and ovary co-culture may be added to the induction medium to enhance embryogenesis from microspores.

Abstract: The current invention provides nucleic acid sequences encoding the RIN and MC genes. Compositions comprising these sequences are described, as are plants transformed with such compositions. Further provided are methods for the expression of the RIN and MC genes. The methods of the invention include the direct creation of transgenic plants with the RIN and MC genes by genetic transformnation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Abstract: The present invention provides novel methods for modifying target nucleotide sequences in a plant cell through the use of homologous recombination. The plant cell is transformed with a DNA construct comprising a polynucleotide sequence encoding a fusion polypeptide comprising a polypeptide sequence of interest linked to a reporter sequence.

Abstract: A transformed cotton plant. The transformed cotton plant comprises DNA derived from a source other than cotton plants, wherein the DNA, when transformed into the cotton plants, confers a phenotype not expressed inn a parent cotton.

Abstract: The subject invention is related to a method for recombinantly and transiently producing a polypeptide in a plant tissue, which includes providing a plant tissue sample in a bioreactor; adding to the plant tissue sample, a sample of Agrobacterium containing a nucleotide sequence encoding the polypeptide on the T-DNA, co-culturing the plant tissue sample with the Agrobacterium so that the nucleotide sequence is transferred to the plant, allowing the plant tissue to transiently express the polypeptide, and then separating the polypeptide from the mixture.

Abstract: This invention relates to an isolated nucleic acid fragment encoding a tetrahydrofolate metabolism enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the tetrahydrofolate metabolism enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the tetrahydrofolate metabolism enzyme in a transformed host cell.

Abstract: The present invention relates to a new gene encoding a glycine-rich polypeptide, and an expression vector, host cell and transgenic plant comprising the gene. The expression of the gene of the invention in the plant will enhance resistance against a wide variety of pathogens, in particular fungi.