Oxygen Of The Saccharide Radical Is Directly Bonded To A Nonsaccharide Heterocyclic Ring Or A Fused- Or Bridged-ring System Which Contains A Nonsaccharide Heterocyclic Ring (e.g., Coumermycin, Novobiocin, Etc.) Patents (Class 435/75)
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Patent number: 5741714Abstract: A method and apparatus for determining qualitatively or quantitatively the presence of analyte bound to a separation media without doing a bound/free separation. In the method, the bound fraction is collected in an assay region of a body of liquid which includes the free analyte, and the assay is performed by comparing the radiant-energy response in the assay region to the radiant-energy response in a control region of the body of liquid which is free of bound analyte. The apparatus has a chamber which contains the body of liquid, one or more collection elements and a control element and position in the body of liquid parallel to an opaque wall which has a colliminating slit in registry with each element. Each slit enables sensing of the radiant-energy response from the body of liquid between the slit and its associated elements.Type: GrantFiled: July 16, 1996Date of Patent: April 21, 1998Assignee: Immunivest CorporationInventor: Paul A. Liberti
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Patent number: 5738984Abstract: A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.Type: GrantFiled: June 2, 1995Date of Patent: April 14, 1998Assignee: Yissum Research Development Company of the Hebrew University of JerusalemInventor: Oded Shoseyov
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Patent number: 5739041Abstract: The invention is an improved single-step test device for detecting the presence of a pre-selected analyte in a urine stream. The device has a hollow outer casing and an assay material disposed within the casing. The outer casing defines: a urine inlet port; a viewing window; and at least one drainage vent spaced about the urine inlet port. The assay material is a sorptive material defining: a urine sample application region adjacent to, and in fluid communication with the urine inlet port; a capture region adjacent to the viewing window; and a fluid flow path for transporting liquid sample between the urine sample application region and the analyte capture region. The drainage vent is located to permit excess urine entering the casing from the urine stream to exit the casing thereby to minimize hydraulic pressure induced flooding of the assay material disposed within the casing and to reduce the frequency of false test results.Type: GrantFiled: May 2, 1995Date of Patent: April 14, 1998Assignee: Carter Wallace, Inc.Inventors: Albert Nazareth, Yea-Shun Cheng, Mary Beth Boyle
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Patent number: 5731158Abstract: The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system.In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.Type: GrantFiled: May 20, 1996Date of Patent: March 24, 1998Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark Norman Bobrow, Gerald Joseph Litt
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Patent number: 5726023Abstract: Methods for the detection, monitoring and treatment of malignancies in which the HER-2/neu oncogene is associated are disclosed. Detection of specific T cell activation (e.g., by measuring the proliferation of T cells) in response to in vitro exposure to the HER-2/neu protein, or detection of immunocomplexes formed between the HER-2/neu protein and antibodies in body fluid, allows the diagnosis of the presence of a malignancy in which the HER-2/neu oncogene is associated. The present invention also discloses methods and compositions, including peptides, for treating such malignancies.Type: GrantFiled: June 6, 1995Date of Patent: March 10, 1998Assignee: University of WashingtonInventors: Martin A. Cheever, Mary L. Disis
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Patent number: 5723303Abstract: The subject invention concerns a novel polynucleotide sequence cloned from emm2.2 gene of a Group A streptococcus, Type II strain which codes for an IgA-binding protein, ML2.2. The subject invention further concerns the novel IgA-binding protein. A process for producing the protein is given. The invention also concerns the protein in an immunoadsorbent and as a tracer for use in measuring and purifying IgA. Kits are given comprising the immunoadsorbent and the tracer form of the protein.Type: GrantFiled: June 14, 1996Date of Patent: March 3, 1998Assignee: Rockefeller UniversityInventors: Vincent A. Fischetti, Debra E. Bessen
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Patent number: 5723306Abstract: Highly selective monoclonal antibodies for pyrethrins and pyrethroids which, in their function as pesticides, contain a phenoxybenzyl and/or a cyclopropane component. The number of pyrethroids which can be selectively detected is increased and the possibility of differentiating among the various pyrethrins, pyrethroids and their metabolites is possible. An easy-to-use test kit detects pyrethrins and pyrethroids.A conjugate of a metabolite of the pyrethrins/pyrethroids (especially permethric acid or phenoxybenzoic acid) is coupled as hapten to a carrier protein to obtain the immunogen, which is used for immunization and for the subsequent cloning of suitable hybridomas.Type: GrantFiled: August 24, 1994Date of Patent: March 3, 1998Assignee: Dragerwerk AktiengesellschaftInventors: Sabine Pullen, Berthold Hock, Thomas Wuske, Andreas Manns
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Patent number: 5723344Abstract: Device for capturing a target molecule for the purpose of detecting it and/or assaying it, including a solid support on which is immobilized a ligand, the ligand being provided in the form of a conjugate resulting from the covalent coupling of a polymer with a plurality of molecules of the ligand. The polymer is an N-vinylpyrrolidone copolymer, and the conjugate is immobilized on the solid support by adsorption.When the ligand is capable of forming a complex with the target, the device is specific for a given target. When the device comprises, in addition, a bifunctional reagent capable of forming a complex, on the one hand, with the ligand and, on the other hand, with the target, the support on which the ligand is immobilized constitutes a universal capturing system.Type: GrantFiled: June 24, 1994Date of Patent: March 3, 1998Assignee: Bio MerieuxInventors: Claude Mabilat, Philippe Cros, Bernard Mandrand, Marie-Helene Charles, Marie-Noelle Erout, Christian Pichot
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Patent number: 5723304Abstract: The invention relates to a method of detection, a sensor and a test-kit which find application in immunological detection (e.g., immunoassay). The invention provides, inter alia, a method of detection, suitable for use in immunological detection of an entity, which method includes the use of a secondary species (as defined in the specification), the use of a first detectable species, and the use of a second detectable species. The method may include, for example, the use of a primary species, a secondary species, a first detectable species and a second detectable species. The primary species may be, for example, an antibody or a ligand. The secondary species may be, for example, an auxiliary species such as an auxiliary binder or an auxiliary ligand, or a species which has a part which is an auxiliary function. The entity to be detected may be an analyte species as such or may be an entity which carries or includes analytes species.Type: GrantFiled: February 27, 1995Date of Patent: March 3, 1998Assignee: GEC-Marconi LimitedInventor: Ramadan Arbi Abuknesha
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Patent number: 5721208Abstract: Novel glycopeptides, a process for their preparation and their useDesmethylbalhimycin, a compound of the formula C.sub.65 H.sub.71 Cl.sub.2 N.sub.9 O.sub.24, desmethylleucylbalhimycin, a compound of the formula C.sub.59 H.sub.60 Cl.sub.2 N.sub.8 O.sub.23, desglucobalhimycin, a compound of the formula C.sub.60 H.sub.63 Cl.sub.2 N.sub.9 O.sub.19, ureidobalhimycin, a compound of the formula C.sub.67 H.sub.74 Cl.sub.2 N.sub.10 O.sub.25, desmethyl-desglucobalhimycin, a compound of the formula C.sub.59 H.sub.61 Cl.sub.2 N.sub.9 O.sub.19 and balhimycin V, a compound of the formula C.sub.73 H.sub.84 Cl.sub.2 N.sub.10 I.sub.26, methylbalhimycin, a compound of the formula C.sub.67 H.sub.75 Cl.sub.2 N.sub.9 O.sub.24 and balhimycin R, a compound of the formula C.sub.72 H.sub.83 Cl.sub.2 N.sub.9 O.sub.28, have an antibiotic action.Type: GrantFiled: June 7, 1995Date of Patent: February 24, 1998Assignee: Hoechst AktiengesellschaftInventors: Laszlo Vertesy, Joachim Betz, Hans-Wolfram Fehlhaber, Michael Limbert
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Patent number: 5719031Abstract: This invention relates to polymers labeled with fluorescent dye to the point that significant fluorescence quenching occurs, such that degradation of the polymer results in fluorescence enhancement. The resulting fluorescence enhancement is useful for measuring the degradation of such polymers, for example as a result of enzymatic hydrolyis of a protein, carbohydrate, nucleic acid, or other natural or synthetic polymer.Type: GrantFiled: August 14, 1996Date of Patent: February 17, 1998Assignee: Molecular Probes, Inc.Inventors: Richard P. Haugland, Mingjie Zhou
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Patent number: 5714327Abstract: The invention provides platinum-based probe compounds having the structure: ##STR1## wherein: Pt is a platinum atom, PROBE is a probe biomolecule for associating to a target biomolecule, M is a detectable marker moiety, and X and Y are stabilizing substituents. Also provided are platinum-based labeling compounds having the structure: ##STR2## wherein: Pt is a platinum atom, M is a detectable marker moiety, A is a displaceable leaving group, and X and Y are stabilizing substituents. The invention further provides platinum-based linker compounds having the structure: ##STR3## wherein: Pt is a platinum atom, A and B are the same or different reactive moieties, and X and Y are stabilizing substituents. Other Pt.sup.II and Pt.sup.IV compounds are also provided. Moreover, the invention provides methods for the preparation and use of these compounds, as well as diagnostic kits which contain the compounds.Type: GrantFiled: June 6, 1995Date of Patent: February 3, 1998Assignee: Kreatech DiagnosticsInventors: Hendrik J. Houthoff, Jan Reedijk, Tinka Jelsma, Remco Maria Van Es, Franciscus Michiel van den Berg, Edwin Leo Marlo Lempers, Marieke Johanna Bloemink
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Patent number: 5708154Abstract: Molecules having an RNA block linked to a shorter DNA block is described. The RNA and DNA blocks are complementary to accommodate formation of fold-back molecules having a 3' hydroxyl on the DNA block and an overhanging RNA strand at the end of a short DNA-RNA hybrid.Type: GrantFiled: August 16, 1991Date of Patent: January 13, 1998Assignee: City of HopeInventors: Steven S. Smith, Bruce E. Kaplan
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Patent number: 5707799Abstract: The present invention relates to analytical devices for determining the presence or amount of an analyte in a test sample. The analytical devices comprise an inlet port, a vent, a channel, and an array of structures. The structures have immobilized reagent covalently or non-covalently attached to the surface of the structures. The immobilized reagent captures analyte in the test sample where it is detected by a detection system. The present invention also provides methods and reagents for performing assays utilizing the analytical devices of the present invention. The present invention also provides methods of manufacturing the analytical devices of the present invention.Type: GrantFiled: September 30, 1994Date of Patent: January 13, 1998Assignee: Abbott LaboratoriesInventors: Douglas D. Hansmann, John P. Grace, Michael G. Lowery, Gary M. Oosta, Neil W. Loomis, Eric B. Shain, Thomas G. Schapira
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Patent number: 5705338Abstract: Novel non-competitive assay techniques have been developed which not only improve sensitivity, but also are convenient and less susceptible to interfering factors. They are compatible with existing instruments and can be run in one or more test tubes. The analyte is reacted with labeled specific binder, after which the mixture is reacted with (1) an insoluble material attached to an analyte derivative and (2) a solid phase carrying a binder. The solid phase is then separated, and the label attached to the solid phase is measured. Variations of the procedure include the use of a reversible bridge for attaching the insoluble material to the analyte mimic and the conduct of the assay in various porous media, such as paper, chromatographic and electrophoretic media, and dipsticks.Type: GrantFiled: May 4, 1995Date of Patent: January 6, 1998Assignee: Chiron Diagnostics CorporationInventors: Uri Piran, Laurie Ann Livshin, Richard A. Martinelli, William J. Riordan, John T. Unger
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Patent number: 5700641Abstract: Synthesized telomeric sequences bind to and can be used for detecting anti-DNA antibodies in serum. Autoimmune diseases such as Lupus Erythematosus, Rheumatoid Arthritis, and Scleroderma, can be detected by detecting an elevated level of anti-DNA antibodies using telomeric sequences. Test kits for such detection are provided including immobilized telomeric sequences capable of binding anti-DNA antibodies. Pharmaceutical compositions for inhibiting or reducing the activity of anti-DNA antibodies contain an effective amount of telomeric sequences effective in inhibiting the antibodies specific to the patient treated.Type: GrantFiled: March 1, 1995Date of Patent: December 23, 1997Inventor: Eeva-Marjatta Salonen
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Patent number: 5700639Abstract: The invention concerns a method for the non-radioactive detection of metabolically labelled DNA, in one aspect detecting labelled DNA indicative of cell lysis or apoptosis.Type: GrantFiled: June 9, 1994Date of Patent: December 23, 1997Assignee: Boehringer Mannheim GmbHInventors: Bernhard Trauth, Matthias Hinzpeter, Clemens Doppler, Eberhard Russmann
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Patent number: 5698405Abstract: The immunogenicity of avidin, a therapeutic agent moiety of a conjugate, or a targeting composition is reduced by coupling the immunogenic agent with a carbohydrate polymer or polyol groups, such as polysaccharides (e.g. dextran), polyethylene glycol and the like.Type: GrantFiled: June 1, 1995Date of Patent: December 16, 1997Assignee: Immunomedics, Inc.Inventor: David M. Goldenberg
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Patent number: 5698420Abstract: Preparation of 4'-deoxy-O-mycaminosyltylonolide by feeding repromicin to a fermentation broth of a strain of the microorganism Streptomyces fradiae (ATCC 31733) under aerobic conditions in an aqueous nutrient medium containing inorganic salts and assimilable sources of carbon and nitrogen.Type: GrantFiled: November 26, 1996Date of Patent: December 16, 1997Assignee: Pfizer Inc.Inventor: Lapyuen H. Lam
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Patent number: 5690907Abstract: A method for ligand-based binding of lipid encapsulated particles to molecular epitopes on a surface in vivo or in vitro comprises sequentially administering (a) a site-specific ligand activated with a biotin activating agent; (b) an avidin activating agent; and (c) lipid encapsulated particles activated with a biotin activating agent, whereby the ligand is conjugated to the particles through an avidin-biotin interaction and the resulting conjugate is bound to the molecular epitopes on such surface. The conjugate is effective for imaging by x-ray, ultrasound, magnetic resonance or positron emission tomography. Compositions for use in ultrasonic imaging of natural or synthetic surfaces and for enhancing the acoustic reflectivity thereof are also disclosed.Type: GrantFiled: June 8, 1995Date of Patent: November 25, 1997Assignee: The Jewish Hospital of St. LouisInventors: Gregory M. Lanza, Samuel A. Wickline
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Patent number: 5691152Abstract: Compositions and methods for avidin immobilized on an inert support material, e.g., agarose, are disclosed. The compositions have high activity levels of avidin and may further include a bulking agent, e.g., maltose, and a protectant to maintain the stability and integrity of the avidin agarose during lyophilization and terminal sterilization processes. These compositions have applicability in any instance where avidin agarose and/or the avidin/biotin technology are useful. In particular, the present compositions are useful in an enzyme capture system to prepare fibrin monomer useful for fibrin sealants.Type: GrantFiled: November 9, 1995Date of Patent: November 25, 1997Assignee: E. R. Squibb & Sons, Inc.Inventors: Steven James Burton, James C. Pearson, Peter A. D. Edwardson
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Patent number: 5686257Abstract: T cell surface antigens from mammals, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding said antigens. Methods of using said reagents and diagnostic kits are also provided.Type: GrantFiled: April 26, 1995Date of Patent: November 11, 1997Assignee: Schering CorporationInventors: Jacqueline Kennedy, J. Fernando Bazan, Albert Zlotnik
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Patent number: 5683895Abstract: The present invention is based upon the discovery that a MeOH extract of Anabaena affinis strain VS-1 showed strong cytotoxicity to L1210 murine leukemia cells, and from that extract was isolated the compounds having the following structures, Compounds 1 and 2. These two compounds are believed to be responsible for the cytotoxicity of the marine organism. This is the first report of the isolation and characterization of pyrrolo?3,2-d!pyrimidine derivatives as biosynthetic products.Type: GrantFiled: June 7, 1995Date of Patent: November 4, 1997Inventors: Kenneth L. Rinehart, Wayne W. Carmichael, Michio Namikoshi
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Patent number: 5679526Abstract: This invention is directed to a ligand-receptor assay for determining the presence of at least one target ligand, capable of competing with a ligand analogue conjugate for binding sites available on a ligand receptor, said ligand analogue conjugate comprising at least one ligand analogue coupled to a colloidal gold particle, in a fluid sample suspected of containing said target ligand comprising the steps of:a. contacting said fluid sample with said ligand analogue conjugate and said ligand receptor to form a homogeneous reaction mixture, the relative amounts of said ligand analogue conjugate and said ligand receptor being selected such that in the absence of said target ligand and subsequent to substantially equilibrium binding in said reaction mixture, substantially all of said ligand analogue conjugate is bound to said ligand receptor such that no unbound ligand analogue conjugate is detected as a result of the assay method;b.Type: GrantFiled: August 1, 1994Date of Patent: October 21, 1997Assignee: Biosite Diagnostics IncorporatedInventors: Kenneth F. Buechler, Gunars E. Valkirs, Richard R. Anderson
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Patent number: 5674677Abstract: Two amino acid sequences are joined together using an electron acceptor moiety and a linking moiety, such as a chelating agent. In particular, an amino acid sequence specific for binding to a material interest is linked to an enzyme which acts on an indicator, such as a colorimetric, phosphometric, fluorometric or chemiluminescent substrate. The linking composition is useful in immunoassays.Type: GrantFiled: December 14, 1995Date of Patent: October 7, 1997Assignee: The Center for Innovative TechnologyInventor: Darrell L. Peterson
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Patent number: 5670364Abstract: New A83543 components, including fermentation products A83543K, A835430, A83543P, A83543U, A83543V, A83543W and A83543Y and N-demethyl derivatives, and salts thereof, are useful for the control of insects and mites. The pseudoaglycones of the new A83543 components are useful for the preparation of A83543 components. Methods are provided for making the new A83543 components by culturing of Saccharopolyspora spinosa NRRL 18395, NRRL 18537, NRRL 18538, or NRRL 18539, or NRRL 18743 or NRRL 18719 or NRRL 18823 in suitable culture medium. Insecticidal and ectoparasiticidal compositions containing new A83543 components are also provided.Type: GrantFiled: June 7, 1995Date of Patent: September 23, 1997Assignee: DowElancoInventors: Jon S. Mynderse, James A. Mabe, Jan R. Turner, Mary L. B. Huber, Mary C. Broughton, Walter M. Nakatsukasa, Lawrence Creemer, Herbert A. Kirst, James W. Martin
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Patent number: 5670345Abstract: Coal is treated aerobically or anaerobically to produce humic acid, volatile fatty acids, lower alcohols, and/or methane using a consortium of bacteria designated Mic-1 or KSARC56. This process can also be used to convert aromatic compounds, such as phenols and derivatives thereof, to methane and carbon dioxide.Type: GrantFiled: June 7, 1995Date of Patent: September 23, 1997Assignee: Arctech, Inc.Inventors: Kailash Chandra Srivastava, Daman S. Walia
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Patent number: 5665539Abstract: A novel system and method for sensitive antigen detection. The system utilizes immuno-polymerase chain reaction in which a specific biotinylated nucleic acid molecule is used as the marker. The biotinylated marker is attached to antigen-antibody complex through a streptavidin-protein A chimeric protein that possesses tight and specific binding affinity both for biotin and immunoglobulin G. A segment of the attached biotinylated marker is amplified by polymerase chain reactions with appropriate primers and the polymerase chain reaction products are detected by agarose gel electrophoresis. The method can detect any antigen and has a greater sensitivity than any existing antigen detection system.Type: GrantFiled: October 4, 1993Date of Patent: September 9, 1997Assignee: The Regents of the University of CaliforniaInventors: Takeshi Sano, Charles R. Cantor, Cassandra L. Smith
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Patent number: 5665559Abstract: A monoclonal antibody is disclosed which is reactive to Bacteroides gingivalis and produced by the hybridoma deposited under ATCC HB 9968. The invention also discloses diagnostic reagents and methods for detecting Bacteroides gingivalis utilizing the hybridoma deposited under ATCC HB 9968.Type: GrantFiled: May 18, 1989Date of Patent: September 9, 1997Assignee: The United States of America as represented by the Secretary of the NavyInventor: Lloyd G. Simonson
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Patent number: 5658725Abstract: Subject matter of the invention are protein-containing interference-reducing agents for immunoassays consisting of protein aggregates that are acylated with --CO--R groups, wherein R is a branched or non-branched C1-C4 alkyl residue which can be substituted with hydroxy, carboxy, SO.sub.3 H or PO.sub.3 H.sub.2 groups. These interference-reducing substances can be used together with a buffer as an interference-reducing agent or together with an immunological binding partner as a binding reagent to reduce non-specific interactions in immunoassays. Another subject matter of the invention is an immunological testing method wherein said interference-reducing substances are used.Type: GrantFiled: August 21, 1995Date of Patent: August 19, 1997Assignee: Boehringer Mannheim GmbHInventors: Dittmar Schlieper, Franz Schmid, Martin Kaufmann
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Patent number: 5643731Abstract: The invention relates to a method of using a pair of leucine zipper peptides for in vitro diagnosis, in particular, for the immunochemical detection and determination of an analyte in a biological liquid. In one method, the first leucine zipper peptide is immobilized by attaching it to a solid support, the second leucine zipper peptide is coupled to a specific binding partner for the analyte, the two peptides are brought into contact, the sample of the biological liquid is brought into contact with the immobilized first peptide and the specific binding partner for the analyte, and the amount of analyte bound to the binding partner is determined. The leucine zipper peptides are preferably v-fos and c-jun.Type: GrantFiled: June 6, 1995Date of Patent: July 1, 1997Assignee: Behringwerke AktiengesellschaftInventors: Klaus Bosslet, Peter Hermentin, Hans Harald Sedlacek, Bernhard Auerbach, Peter Pfleiderer, Rolf Muller
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Patent number: 5639597Abstract: The invention relates to cell-free receptor binding assays which permit the binding behavior of receptor proteins in the cell membrane toward natural or artificial ligands to be investigated. This entails the particular receptor being linked to a suitable carrier molecule, preferably the heavy chain of an immunoglobulin, and being bound via the carrier, with retention of its biological property, to a suitable solid phase.Type: GrantFiled: May 13, 1994Date of Patent: June 17, 1997Assignee: Behringwerke AktiengesellschaftInventors: Leander Lauffer, Gerd Zettlmeissl, Patricia Oquendo
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Patent number: 5637465Abstract: The invention concerns a method for the detection of a programmed or induced cell death of eukaryotic cells as well as a suitable test kit for this method of detection.Type: GrantFiled: May 18, 1994Date of Patent: June 10, 1997Assignee: Boehringer Mannheim GmbHInventor: Bernhard Trauth
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Patent number: 5637508Abstract: Polymer or copolymer coated catalytic colloidal metal particles bound to a biomolecule such as an antibody, avidin, or streptavidin and kits containing such polymer or copolymer coated catalytic metal particles are useful for detecting the presence of the biomolecule in an assay such as an immunoassay.Type: GrantFiled: January 23, 1995Date of Patent: June 10, 1997Assignees: Geo-Centers, Inc., The United States of America as represented by the Secretary of the NavyInventors: David A. Kidwell, Susan M. Conyers
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Patent number: 5635363Abstract: T cells are specifically labeled according to their antigen receptor by binding of a multimeric binding complex. The complex is prepared with major histocompatibility complex protein subunits having a homogeneous population of peptides bound in the antigen presentation site. The multimeric MHC-antigen complex forms a stable structure with T cells, thereby allowing for the labeling, identification and separation of specific T cells.Type: GrantFiled: February 28, 1995Date of Patent: June 3, 1997Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: John D. Altman, Michael G. McHeyzer-Williams, Mark M. Davis
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Patent number: 5629213Abstract: A biosensor for use in a surface plasmon resonance (SPR) System includes a transparent substrate layer, a thin metallic film on the substrate, and an ultrathin organic layer of a material which is polyanionic and adsorbs on the metallic film, and a layer of polylysine on this polyanionic material. In one embodiment, there is an outer layer on the polylysine which binds with a specific desired analyte.Type: GrantFiled: March 3, 1995Date of Patent: May 13, 1997Inventors: Steven E. Kornguth, Robert M. Corn, Claire E. Jordan, Brian L. Frey
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Patent number: 5627078Abstract: The present invention concerns a multivalent dextran reagent for use in a precipitation test for the determination of a specifically bindable substance comprising dextran to which several molecules of a receptor R.sub.1 which is capable of specific binding to the substance to be determined or of the specifically bindable substance or of an analogue of this substance are bound or can be bound.Type: GrantFiled: June 1, 1994Date of Patent: May 6, 1997Assignee: Boehringer Mannheim GmbHInventors: Johann Karl, Josef Maier
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Patent number: 5627080Abstract: Methods for modulating the rates and dose responses of immunoassays through the incorporation of one or more detergents into the immunoassay reaction are disclosed. The methods are particularly suitable for automated immunoassay formats, especially with formats that use analyte-biotin bidentate reagents. The methods may be used to facilitate the detection of any desired, preselected pharmacological agent.Type: GrantFiled: July 29, 1994Date of Patent: May 6, 1997Assignee: Beckman Instruments, Inc.Inventors: Anthony K. Cheng, Julie S. Kim, Chan S. Oh
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Patent number: 5622872Abstract: Bioelectronic sensors are provided employing a thin surfactant polymeric electrically conducting layer to which may be bound members of specific binding pairs. Binding of an analyte or a reagent to the specific binding pair member layer may change the electrical, optical, or structural properties of the layer for measurement of analyte. The change in the polymeric layer provides for a sensitive measurement.Type: GrantFiled: May 11, 1995Date of Patent: April 22, 1997Assignee: Biocircuits CorporationInventor: Hans O. Ribi
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Patent number: 5620842Abstract: A rapid, simple method for preparing beads for calibrating flow cytometers which contain a known number of fluorophores per bead is presented. Briefly, the invention utilizes beads coated with a stable complex of a fluorophore and an enzyme. the enzymatic activity of a known number of beads gives an accurate measure of fluorophore density on those beads.Type: GrantFiled: March 29, 1995Date of Patent: April 15, 1997Assignee: Becton Dickinson and CompanyInventors: Kenneth A. Davis, James E. Bishop
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Patent number: 5618680Abstract: It is an object of the present invention to provide the clinicians with a new application for ligands specific to MHC-class I antigens, especially HLA-ABC antigens, this new application residing in the detection and diagnosis of endometriosis. It is also an object of the present invention to provide a method and a test kit for diagnosing endometriosis, preferably by immunohistochemistry, using a monoclonal anti-HLA-ABC antibody as a preferred ligand or diagnostic reagent. This new method is non-invasive and is more reliable as a screening test than the conventionnally used laparoscopy. When the endometrium of a woman tests negatively with the claimed method, it prevents the use of laparoscopy which is an invasive method for detecting endometriosis. This method can be practiced on a specimen obtained from the endometrium of a patient and does not require a specimen sampled directly from the endometriotic foci.Type: GrantFiled: December 28, 1994Date of Patent: April 8, 1997Assignee: Institut de Medecine de la Reproduction de MontrealInventors: Pierre Miron, Denis-Claude Roy, Marie-Helene Lachapelle
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Patent number: 5616458Abstract: The present invention provides for the use of Tripterygium wilfordii Hook F extracts and purified components thereof in the treatment of inflammation or an immune disorder with concomitant lack of steroidal effect. Extracts of this plant (T2) bound to the glucocorticoid receptor and competitively inhibited glucocorticoid mediated cellular processes, such as dexamethasone binding to the glucocorticoid receptor, glucocorticoid mediated activation of target genes, dexamethasone dependent cellular growth, with concomitant inhibition of cyclooxygenase-2 induction and inflammatory processes such as the production of prostaglandin E.sub.2. The T2 extract components triptolide and tripdiolide were effective inhibitors. The particular advantage provided by the methods herein is the treatment or prevention of inflammation and the concomitant lack of steroidal agonist effects and NSAID side effects. Conditions treatable by the present methods include inflammation and immune disorders including autoimmune disease.Type: GrantFiled: May 31, 1995Date of Patent: April 1, 1997Assignee: Board of Regents, University of TX SystemInventors: Peter E. Lipsky, Xue-Lian Tao, Jian Cai, William J. Kovacs, Nancy J. Olsen
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Patent number: 5614368Abstract: Compounds and methods for detecting the incorporation of a hapten into a protein or macromolecule are disclosed. The compounds comprise haptens bound directly or indirectly to a chromophoric group and a macromolecule-reactive group, whereby incorporation of the hapten into a protein or other macromolecule may be detected.Type: GrantFiled: December 23, 1994Date of Patent: March 25, 1997Assignee: Molecular Devices CorporationInventors: Vartan Ghazarossian, Viola T. Kung, Robert F. Zuk
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Patent number: 5612221Abstract: Avidin-binding azo reagents which alter the spectrophotometric properties of avidin and the use of such reagents in homogeneous assays are described.Type: GrantFiled: May 30, 1995Date of Patent: March 18, 1997Assignee: Dade Chemistry Systems Inc.Inventors: Donald M. Simons, Susan Y. Tseng, Patricia C. Weber
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Patent number: 5591606Abstract: New A83543 components, including fermentation products A83543Q, A83543R, A83543S and A83543T and N-demethyl derivatives, and salts thereof, are useful for the control of insects and mites. The pseudoaglycones are useful for the preparation of A83543 components. Methods for making the new A83543 components by culture of Saccharopolyspora spinosa NRRL 18823 are provided. Insecticidal and ectoparasiticidal compositions containing new A83543 components are also provided.Type: GrantFiled: March 2, 1995Date of Patent: January 7, 1997Assignee: DowElancoInventors: Jan R. Turner, Mary L. B. Huber, Mary C. Broughton, Jon S. Mynderse, James W. Martin
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Patent number: 5591647Abstract: Membrane for use in the detection of an analyte having a closely packed array of self-assembling amphiphilic molecules and a plurality of ionophores. First and second ligands are attached to an end of the ionophores adjacent the surface of the membrane. The membrane is such that the binding of the first ligand to its specific binding partner prevents the flow of ions across the membrane via the ionophores. In addition, the binding of the second ligand to its specific binding partner prevents the binding of the first ligand to its specific binding partner.Type: GrantFiled: May 17, 1995Date of Patent: January 7, 1997Assignees: Australian Membrane and Biotechnology Research Institute, The University of SydneyInventor: Lionel G. King
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Patent number: 5585251Abstract: Novel antifungal agents having the formula: ##STR1## wherein R is hydrogen or a radical of the formula ##STR2## and pharmaceutically acceptable prodrugs thereof, as well as (i) pharmaceutical compositions comprising such compounds, (ii) methods of treatment using such compounds, and (iv) methods and fungal cultures useful in making the same.Type: GrantFiled: January 10, 1995Date of Patent: December 17, 1996Assignee: Abbott LaboratoriesInventors: Lisa A. Alder, Neal S. Burres, Jill E. Hochlowski, Marianna Jackson, James B. McAlpine
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Patent number: 5583001Abstract: The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system.In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.Type: GrantFiled: May 4, 1994Date of Patent: December 10, 1996Assignee: E. I. du Pont de Nemours and CompanyInventors: Mark N. Bobrow, Gerald J. Litt
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Patent number: 5583015Abstract: Streptomyces avermitilis lacking branched-chain amino acid transaminase activity and/or branched-chain 2-oxo acid dehydrogenase activity, methods for preparation thereof, and use thereof to produce natural and non-natural avermectins useful as parasiticides.Type: GrantFiled: October 14, 1994Date of Patent: December 10, 1996Assignee: Pfizer Inc.Inventors: Edmund W. Hafner, Kelvin S. Holdom, S. Edward Lee
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Patent number: 5583029Abstract: Mutants of Streptomyces avermitilis lacking ability to produce glycosylated avermectins and lacking branched-chain 2-oxo acid dehydrogenase activity, method for preparation thereof, and use thereof to produce natural and non-natural avermectin aglycones useful as parasiticides.Type: GrantFiled: October 14, 1994Date of Patent: December 10, 1996Assignee: Pfizer Inc.Inventors: Lapyuen H. Lam, Hamish A. I. McArthur, Richard G. Wax