Utilizing Paper Or Thin Layer Plate Patents (Class 436/162)
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Patent number: 6534323Abstract: The invention relates to methods, compositions, kits, and devices for detecting cardiac ischemia, hypoxia, or other causes of heart failure in a mammal by obtaining a test sample from a mammal, measuring a level of a non-polypeptidic cardiac marker in the test sample, and determining if the level of the cardiac marker measured in said test sample correlates with cardiac ischemia or hypoxia or another form of heart failure.Type: GrantFiled: January 21, 2000Date of Patent: March 18, 2003Assignee: Medlyte Diagnostics, Inc.Inventor: Roger Sabbadini
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Patent number: 6534322Abstract: The invention relates to methods, compositions, kits, and devices for detecting cardiac ischemia, hypoxia, or other causes of heart failure in a mammal by obtaining a test sample from a mammal, measuring a level of a non-polypeptidic cardiac marker in the test sample, and determining if the level of the cardiac marker measured in said test sample correlates with cardiac ischemia or hypoxia or another form of heart failure.Type: GrantFiled: January 21, 2000Date of Patent: March 18, 2003Assignee: Medlyte Diagnostics, Inc.Inventor: Roger A. Sabbadini
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Publication number: 20030017606Abstract: The present invention is a very significant way to determine exposure to toxic mold, or the disease called mycotoxicosis. Urine samples are extracted for trichothecene mycotoxins to confirm the disease of mycotoxicosis in humans. Diagnosis of mycotoxicosis is confirmable even when toxic mold exposure cannot be ascertained to a reasonable degree of certainty.Type: ApplicationFiled: July 20, 2001Publication date: January 23, 2003Inventor: William A. Croft
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Publication number: 20020173047Abstract: The present invention recognizes that it can be desirable to have a sample receiving chamber integral to or engageable with a test platform, such as a test platform that includes a test strip. The sample receiving chamber is preferably separate or separable from the test platform, but that need not be the case. Preferably, a fluid flow actuating device or structure, such as a valve separates the sample receiving chamber from the test platform. The present invention provides such a device and methods of use. A first aspect of the present invention is a test device that includes a sample receiving chamber and a test platform that preferably includes a test element. The sample receiving chamber preferably engages the test platform and is optionally separable therefrom. A second aspect of the present invention is a method of detecting an analyte in a sample, including: providing a sample, contacting the sample with a test device and detecting the analyte in the sample.Type: ApplicationFiled: May 18, 2001Publication date: November 21, 2002Inventors: Robert Thomas Hudak, Lorraine Bautista
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Publication number: 20020155029Abstract: A device for collecting and transporting aqueous fluid from the oral cavity to a lateral chromatographic strip for test is disclosed. The lateral chromatographic strip is placed within and extend along a cavity defined in a housing. At least one inspection site to the lateral chromatographic strip is provided to enable inspection of selected sites on the lateral chromatographic strip for test results. A porous wick material protrudes from the housing to a collection site exterior of the housing at one end and communicates to the lateral chromatographic strip at the other end. The porous wick material has particulate construction, the particles adsorbing aqueous oral fluid to transport the fluid from the mouth to the lateral chromatographic strip without substantial absorption. The particles of the porous wick material are bound together to define a continuous interstitial volume for the flow of oral fluid to be transported and are treated to be hydrophilic to the adsorbed oral fluids.Type: ApplicationFiled: October 11, 2001Publication date: October 24, 2002Applicant: Epitope, Inc.Inventors: Ronald W. Mink, Andrew S. Goldstein
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Publication number: 20020137223Abstract: Amlodipine aspartate and amlodipine maleamide are used as reference standards or markers in determining the purity of amlodipine maleate substances and products.Type: ApplicationFiled: August 27, 2001Publication date: September 26, 2002Inventors: Jacobus M. Lemmens, Theodorus H.A. Peters, Peter F.A. Bakker, Frantisek Picha
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Patent number: 6429021Abstract: The present invention provides an assay for detecting the presence of an array or a profile of antioxidant activity in any sources or products. In particular, the invention provides a method in which a sample is extracted with a polar, a non-polar and a semi-polar solvent to obtain three extracts; the extracts are chromatographed to get a chemical profile; and the profile is analyzed to determine the presence of antioxidants by directly reacting to a free-radical.Type: GrantFiled: March 19, 1998Date of Patent: August 6, 2002Assignee: Amway CorporationInventors: Yong Qian, Richard E. A. Leitz, David W. Krempin
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Patent number: 6420181Abstract: A method, system and kit for detecting the presence of an analyte includes placing a solution containing the analyte in a microcapillary tube and placing the microcapillary tube in contact with a layer of sorbent material so that the solution is withdrawn from the microcapillary tube by capillary action. The sorbent material and solvent for the solution are selected so that the solvent is absorbed into the sorbent material and the analyte is adsorbed by the sorbent material and concentrated at the spot where the microcapillary tube contacts the sorbent material. A detector reagent is applied to the sorbent material to indicate the presence of the analyte.Type: GrantFiled: April 23, 1999Date of Patent: July 16, 2002Assignee: The United States of America as represented by the Secretary of the ArmyInventor: Thaddeus John Novak
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Patent number: 6395178Abstract: Novel separation materials for thin layer chromatography are disclosed; these consist of a support and a separation medium layer and are obtainable by the following process steps: a) cleaning of the support; b) application of a liquid film comprising a polysilicic ester to a support; c) introduction of the support with the liquid film into an atmosphere which effects hydrolysis and further polymerization of the polysilicic ester; d) hydrolysis and further polymerization of the polysilicic ester at constant temperature; e) washing of the silica layer; f) drying of the silica layer. The silica gel surface of the separation material of the invention can be modified by means of separation effectors.Type: GrantFiled: August 4, 2000Date of Patent: May 28, 2002Assignee: Merck Patent Gesellschaft mit beschrankter HaftungInventors: Heinz-Emil Hauck, Rolf Eymann, Günther Sättler
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Patent number: 6333198Abstract: A method of testing the purity or stability to degradation of a sample of lamotrigine or a pharmaceutical dosage form comprising lamotrigine comprises assaying the said sample for the presence of a compound selected from 3-amino-6-(2,3-dichlorophenyl)-1,2,4-triazine-5-(4H)-one (compound A) and N-[5-amino-6-(2,3-dichlorophenyl)-1,2,4-triazine-3-yl]-2,3-dichlorobenzamide (compound B). A process for producing compound B, which is novel, is also disclosed.Type: GrantFiled: March 10, 1999Date of Patent: December 25, 2001Assignee: Glaxo Wellcome, Inc.Inventors: Lorraine Mary Edmeades, Nigel Arthur Griffith-Skinner, Derek Anthony Hill, Graham Thronton Hill, Terence William Packham
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Patent number: 6331254Abstract: Methods which employ thin layer chromatography for separating and detecting hydrophobic target molecules are particularly useful in separating biologically relevant lipids. By utilizing non-destructive detection techniques, these methods also can be adapted to further quantification or structural analysis.Type: GrantFiled: December 17, 1999Date of Patent: December 18, 2001Assignee: Cell Therapeutics, Inc.Inventors: Thayer White, Edward Nudelman
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Patent number: 6306665Abstract: Microporous solid phase materials that are suitable for lateral flow and other assays for detecting the presence of analytes in test samples, that are stable under variations in humidity and, even after storage for extended periods of time, can form stable covalent bonds with molecules containing a free primary or secondary amine group or sulfhydryl group are described. The invention further concerns chemically derivatized solid phase materials, and conjugates comprising such materials. Examples of lateral flow devices for the quantitative or semi-quantitative determination of an analyte in a biological sample are described.Type: GrantFiled: October 13, 1999Date of Patent: October 23, 2001Assignee: A-Fem Medical CorporationInventors: Robert Lee Buck, Huiying Wang, Timothy Patrick Hyatt, Paul Andrew Mueggler
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Patent number: 6303081Abstract: A device for collecting and transporting aqueous fluid from the oral cavity to a lateral chromatographic strip for test is disclosed. The lateral chromatographic strip is placed within and extend along a cavity defined in a housing. At least one inspection site to the lateral chromatographic strip is provided to enable inspection of selected sites on the lateral chromatographic strip for test results. A porous wick material protrudes from the housing to a collection site exterior of the housing at one end and communicates to the lateral chromatographic strip at the other end. The porous wick material has particulate construction, the particles adsorbing aqueous oral fluid to transport the fluid from the mouth to the lateral chromatographic strip without substantial absorption. The particles of the porous wick material are bound together to define a continuous interstitial volume for the flow of oral fluid to be transported and are treated to be hydrophilic to the adsorbed oral fluids.Type: GrantFiled: April 15, 1999Date of Patent: October 16, 2001Assignee: OraSure Technologies, Inc.Inventors: Ronald W. Mink, Andrew S. Goldstein
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Patent number: 6287521Abstract: Non-invasive methods are provided for obtaining biological samples of mammary fluid or mammary fluid components by administering oxytocin to a patient to stimulate expression of mammary fluid. During or after mammary fluid expression, a biological sample is collected in the form of whole mammary fluid, whole cells or cellular components, other selected liquid or solid fractions of the mammary fluid, purified or bulk proteins, glycoproteins, peptides, nucleotides or other desired Constituents of mammary fluid. Methods and kits are also provided for determining the presence or amount of a breast disease marker in biological samples of mammary fluid or mammary fluid components obtained according to the above sample collection methods. Also provided within the invention are novel breast pump and breast pump adapter devices which incorporate a solid phase sample collection medium integrated within the breast pump or adapter or otherwise fluidly connected therewith.Type: GrantFiled: February 20, 1998Date of Patent: September 11, 2001Assignee: Atossa Healthcare, Inc.Inventors: Steven C. Quay, Debra L. Quay
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Patent number: 6264893Abstract: An apparatus for conducting multiple thin layer chromatographic processes including an array of receptacles, each receivable of chromatographic fluid, a support structure for supporting the receptacles, and a retaining unit for retaining thin layer chromatographic plates while enabling each plate to be inserted into and removed from a respective receptacle. In use, one or more samples of interest are spotted onto multiple thin layer chromatography plates, the chromatography receptacles are filled with a suitable amount of a chromatography solvent, the plates are inserted into respective chambers to begin the chromatography process and the processes are allowed to continue for a sufficient time period for sample separation to occur. Any separated samples are readily visualized and detected.Type: GrantFiled: April 13, 1999Date of Patent: July 24, 2001Inventor: Larry J. Markoski
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Patent number: 6238928Abstract: The mixture to be tested for toxic components is first separated into fractions by chromatography. The separated fractions are then contacted with luminescent microorganisms in the chromatographic system itself and the toxicity of the fractions is determined by measurement of the bioluminescence. The chromatographic separation may be carried out either by thin layer chromatography or by column liquid chromatography.Type: GrantFiled: September 2, 1993Date of Patent: May 29, 2001Assignee: Bayer AktiengesellschaftInventors: Claus Weisemann, Wolfgang Kreiss, Hans-Georg Rast, Günther Eberz
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Patent number: 6225132Abstract: A method for detecting an analyte of interest present in a mixture at an ultralow concentration includes selecting a radioactive derivatizing agent comprising a multiphoton-emitting radioisotope moiety and a moiety reactive with the analyte of interest, the radioisotope moeity being bound to the derivatizing agent by a bond that is stable under the conditions employed in the other steps of the method, derivatizing the analyte of interest with the derivatizing agent, separating the analyte of interest from other components of the mixture by chromatography, and detecting the analyte of interest using multiphoton detection. The derivatizing step may be performed before or after fractionation. A radiophore for multiphoton emission enhanced chromatography has a first moeity bound to a multiphoton-emitting radioisotope, and a second moiety that is reactive with a functional group of an analyte of interest.Type: GrantFiled: December 10, 1998Date of Patent: May 1, 2001Assignee: BioTraces, Inc.Inventors: Andrzej K. Drukier, Roman Bielski
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Patent number: 6218134Abstract: A specific binding assay process which is useful for quick qualitative or quantitative measurement and which can be used for various purposes, and a specific binding assay device suitable for the practice of the process, in which a substance to be assayed in a liquid test sample is determined qualitatively or quantitatively by developing the substance in a liquid test sample in a matrix, which comprises the steps of, for example: allowing the substance to be assayed to reach with a specific binding substance which has specific affinity for the substance to be assayed; allowing a signal substance generator (a substance which competes with the substance to be assayed for the specific binding substance and which generates a signal) to change its distribution in the matrix in response to the reaction of the above step, and; detecting the resulting distributional changes at a detection means as changes of signals which are rate-limited by the mass transfer of a signal substance generated from the signal substaType: GrantFiled: April 26, 1996Date of Patent: April 17, 2001Assignee: Mochida Pharmaceutical Co., Ltd.Inventors: Tadakazu Yamauchi, Toshinori Kanamori, Masahiro Nobuhara
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Patent number: 6096723Abstract: Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5', 7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.Type: GrantFiled: March 23, 1999Date of Patent: August 1, 2000Assignee: The Perkin-Elmer CorporationInventors: Steven M. Menchen, Linda G. Lee, Charles R. Connell, N. Davis Hershey, Vergine Chakerian, Sam L. Woo, Steven Fung
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Patent number: 6017722Abstract: Methods for the isolation and identification of a toxicant in a sample are disclosed. Luminescent biological agents (i.e., bacteria) having sensitivity to a toxicant or an isolatable component in a sample are used to provide visually discernable zones of luminescent inhibition in the presence of a toxicant (or) in the presence of an isolatable sample component as separated by paper or thin layer chromatography. Kits for use in conjunction with the identification of a toxicant in a sample are also described, which include a luminescent biological reagent as the visualizing agent. Particular examples of luminescent agents include photobacterium leoganthi, photobacterium phosphoreum, Vibrio fischeri, Vibrio harveyi a luminescent fungi, a luminescent fish extract, a luminescent dinoflagellate and fluorescent microorganisms, such as Cypridina. Potential toxicants in a liquid sample, a solid sample, or in a gaseous sample may be identified and further chemically characterized using the described methods.Type: GrantFiled: April 4, 1991Date of Patent: January 25, 2000Assignee: Board of Regents, The University of Texas SystemInventors: James E. Becvar, Laura E. Becvar
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Patent number: 5994141Abstract: Methods for diagnosing and screening for ovarian carcinomas in a subject are provided The methods employ the steps of preparing a plasma sample from a blood specimen from the subject, and assaying for the presence of lysophosphatidic acid in the plasma sample. The presence of lysophosphatidic acid in the plasma sample is indicative of ovarian carcinoma in the subject. In a preferred embodiment the plasma sample is prepared under conditions which minimize the release of lysophosphatidic acid from platelets in the blood specimen into the plasma.Type: GrantFiled: August 25, 1998Date of Patent: November 30, 1999Assignee: The Cleveland Clinic FoundationInventors: Yan Xu, Graham Casey, Douglas C. Gaudette, Bruce J. Holub, Gordon B. Mills
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Patent number: 5958785Abstract: Chronic or long-term alcohol consumption is detected and diagnosed by determining the levels of an alcohol-specific ethanol glycoconjugate biomarker, identified as ethyl .beta.-glucuronide, in body fluids (e.g. urine) of subjects by calorimetric reaction using qualitative and quantitative assay methods. Ethyl glucuronide has been newly observed and detected as a direct indicator of chronic alcohol consumption, and can be isolated and purified. Economical and reproducible assay methods, such as a chromatographic spot assay, ascending or thin layer chromatography assay, and high pressure liquid chromatography assay provide reliable, objective, and sensitive methods for detecting and monitoring a chronic alcoholic condition. Both the presence of the alcohol-specific ethyl glucuronide and a substantial increase in its levels are diagnostic of chronic alcoholism.Type: GrantFiled: August 22, 1997Date of Patent: September 28, 1999Assignee: Research Foundation for Mental HygieneInventors: Raju K. Pullarkat, Premilia S. Pullarkat, Simhachalam Raguthu
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Patent number: 5935862Abstract: Methods of rapidly detecting the presence of chemical warfare agents, cheal warfare agent precursors and degradation products thereof are disclosed. The methods are useful, for example, in screening chemical sample unknowns in Chemical Warfare Convention (CWC) inspections. The methods include contacting a sample suspected of containing chemical warfare agents, precursors, chemical warfare agent degradation products and mixtures thereof with a sufficient amount of a chromatographic adsorbent material and a sufficient amount of a chromogenic detector reagent whereby a chromogenic indicator is formed when the sample contains chemical warfare agent, precursors or degradation product thereof. A kit useful for carrying out the method is also disclosed. The test methods can be used, either alone or in combination with thin-layer chromatography, to provide presumptive evidence for CWC analytes without actually identifying any of the components of the samples.Type: GrantFiled: December 11, 1996Date of Patent: August 10, 1999Assignee: The United States of America as represented by the Secretary of the ArmyInventor: Thaddeus J. Novak
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Patent number: 5885786Abstract: The present invention provides for the screening of candidate substances to identify active compounds that inhibit multidrug resistance (MDR). The expression of glucosylceramides has been determined to be a marker of MDR. By measuring glucosylceramide expression in cells exhibiting MDR, and the reduction in glucosylceramide levels in the presence of a candidate substance, the present invention provides for the identification of MDR inhibitory compounds.Type: GrantFiled: April 19, 1996Date of Patent: March 23, 1999Assignee: John Wayne Cancer InstituteInventor: Myles Cabot
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Patent number: 5882939Abstract: The invention concerns separating materials for thin-layer chromatography, the materials having a sorbent layer formed from sorbent particles and disposed on a carrier. A reflection intensifier, preferably magnesium tungstate and/or Bi.sub.2 O.sub.3, is added to the sorbent layer. This addition comprises at least 10 wt % relative to the amount of sorbent. In preferred embodiments, the particle size of the reflection intensifier is 3 to 5 .mu.m and the amount of the reflection intensifier is 0.6 to 15 times the amount of sorbent. These separating materials are particularly suitable for the in-situ measurement of FTIR spectra. The invention also concerns the use of these separating materials for the in-situ measurement of FTIR spectra.Type: GrantFiled: September 18, 1997Date of Patent: March 16, 1999Assignee: Merck Patent Gesellschaft mit Beschrankter HaftungInventors: Karl Artur Kovar, Walter Fischer, Heinz Emil Hauck
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Patent number: 5854084Abstract: A method for detecting an analyte of interest present in a mixture at an ultralow concentration includes selecting a radioactive derivatizing agent comprising a multiphoton-emitting radioisotope moiety and a moiety reactive with the analyte of interest, the radioisotope moeity being bound to the derivatizing agent by a bond that is stable under the conditions employed in the other steps of the method, derivatizing the analyte of interest with the derivatizing agent, separating the analyte of interest from other components of the mixture by chromatography, and detecting the analyte of interest using multiphoton detection. The derivatizing step may be performed before or after fractionation. A radiophore for multiphoton emission enhanced chromatography has a first moeity bound to a multiphoton-emitting radioisotope, and a second moiety that is reactive with a functional group of an analyte of interest.Type: GrantFiled: July 12, 1996Date of Patent: December 29, 1998Assignee: BioTraces, Inc.Inventors: Andrzej K. Drukier, Roman Bielski
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Patent number: 5840498Abstract: The present invention provides substantially purified cryptdin peptides having a consensus amino acid sequence:X.sub.1 -C-X.sub.2 -C-R-X.sub.3 -C-X.sub.4 -E-X.sub.5 -C-X.sub.6 -C-C-X.sub.7wherein X.sub.1 is 3 to 9 amino acids; X.sub.2 is one amino acid, preferably Y, H or R; X.sub.3 is 2 or 3 amino acids; X.sub.4 is three amino acids; X.sub.5 is five amino acids; X.sub.6 is 6 to 10 amino acids; and X.sub.7 is 0 to 9 amino acids. The invention also provides a substantially purified mouse cryptdin having a consensus amino acid sequence:X.sub.1 -L-X.sub.2 -C-Y-C-R-X.sub.3 -C-K-X.sub.4 -E-X.sub.5 -G-T-C-X.sub.6 -C-C-X.sub.7wherein X.sub.1 is 3 or 4 amino acids, preferably LRD, LSKK (SEQ ID NO: 1) or LRG; X.sub.2 is 1 amino acid, preferably V, L or I; X.sub.3 is 3 amino acids, preferably KGH or *RG, where * is S, T, K, I or A; X.sub.4 is 2 amino acids, preferably GR, RR or RG; X.sub.5 is 3 amino acids, preferably RMN, RVR, RVF HMN or HIN; X.sub.Type: GrantFiled: June 7, 1995Date of Patent: November 24, 1998Assignee: The Regents of the University of CaliforniaInventors: Michael E. Selsted, Andre J. Ouellette
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Patent number: 5824555Abstract: The present invention provides a new method for detecting the presence of gynecological carcinomas in a patient. The method, which comprises detecting the presence of lysophosphatidic acid in a plasma sample of the patient, is useful for detecting ovarian carcinoma, cervical carcinoma, endometrial carcinoma, and peritoneal carcinoma. In a preferred embodiment the method comprises: providing a blood specimen from the patient, obtaining a plasma sample for the blood specimen under conditions which minimize the release of lysophosphatidic acid from the platelets in the blood specimen into the plasma, extracting lipids from the plasma, and detecting the presence of lysophosphatidic acid in the lipid extract. A new method for extracting lysophosphatidic acid from a biological fluid is also provided.Type: GrantFiled: May 30, 1996Date of Patent: October 20, 1998Assignee: The Cleveland Clinic FoundationInventors: Yan Xu, Graham Casey
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Patent number: 5798266Abstract: Non-invasive methods and kits are provided for obtaining biological samples of mammary fluid or mammary fluid components by administering oxytocin or an oxytocin analogue to a mammalian patient to stimulate expression of mammary fluid. The oxytocin is preferably administered intranasally and causes myoepithelial contraction of target alveolar-ductal tissues of the breast. During or after mammary fluid expression, a biological sample is collected in the form of whole mammary fluid, whole cells or cellular components, other selected liquid or solid fractions of the mammary fluid, purified or bulk proteins, glycoproteins, peptides, nucleotides or other desired constituents of mammary fluid. Methods and kits are also provided for determining the presence or amount of a breast disease marker in biological samples of mammary fluid or mammary fluid components.Type: GrantFiled: August 27, 1996Date of Patent: August 25, 1998Assignee: K-Quay Enterprises, LLCInventors: Steven C. Quay, Debra L. Quay
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Patent number: 5780237Abstract: There is disclosed a diagnostic assay for adult respiratory distress syndrome (ARDS), sepsis, multiple organ dysfunction (MOD) and systemic inflammatory response syndrome (SIRS), comprising (a) measuring the amount of selected unsaturated free fatty acids (FFAs) and saturated FFAs in a body fluid, and (b) determining a ratio value comprising the sum of the unsaturated FFAs divided by the sum of the saturated FFAs. There is further disclosed a diagnostic assay for ARDS, sepsis, MOD and SIRS, comprising (a) measuring the amount of 9- or 13-hydroxyoctadecadienoic acid (HODE) and 5-hydroxy-6-trans-8,11,14-cis-eicosatetraenoic acid (5-HETE) in a body fluid, and (b) determining a ratio value of HETE and HODE.Type: GrantFiled: October 12, 1994Date of Patent: July 14, 1998Assignee: Cell Therapeutics, Inc.Inventors: Stuart L. Bursten, David A. Federighi
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Patent number: 5773576Abstract: Support materials for thin-layer chromatography characterized by a marker code applied using laser light.Type: GrantFiled: May 15, 1996Date of Patent: June 30, 1998Assignee: Merck Patent Gesellschaft mit Beschrankter HaftungInventors: Andrea Junker-Buchheit, Heinz-Emil Hauck, Willi Jost
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Patent number: 5763283Abstract: A new class of techniques been developed which allow inexpensive application of SAW-type chemical sensor devices while retaining high sensitivity (ppm) to chemical detection. The new techniques do not require that the sensor be part of an oscillatory circuit, allowing large concentrations of, e.g., chemical vapors in air, to be accurately measured without compromising the capacity to measure trace concentrations. Such devices have numerous potential applications in environmental monitoring, from manufacturing environments to environmental restoration.Type: GrantFiled: October 12, 1994Date of Patent: June 9, 1998Assignee: Sandia CorporationInventors: Richard W. Cernosek, Gregory C. Frye, Stephen J. Martin
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Patent number: 5762877Abstract: The present invention advances a chemical concentrating device for use with thin layer chromatographic, gas chromatographic, high pressure liquid chromatographic, and capillary electrophoresis analysis. The concentrating device has a T-shaped member having a first and second annular branch in axial alignment and a third branch perpendicular to the first and second branches. When used in thin layer chromatographic analysis, the T-shaped member is placed over the syringe needle with the end of the needle extending below the second branch. A gas source in fluid communication with the third branch forwards gas in an annular pattern about the end of the needle, thereby evaporating the solvent within the sample and confining the sample to a particular geometrical area on the thin layer chromatography plate. When used with other chromatographic techniques, the T-shaped member is removably affixed to a syringe needle having a beveled end.Type: GrantFiled: October 18, 1996Date of Patent: June 9, 1998Inventor: William E. Brewer
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Patent number: 5747346Abstract: Chronic or long-term alcohol consumption is detected and monitored by determining the level of a newly-observed, alcohol-specific carbohydrate in body fluids (e.g. urine) of subjects by calorimetric reaction using qualitative and quantitative assay methods. The alcohol-specific carbohydrate have been identified as a novel ethyl glucuronide. Ethyl glucuronide is observed and detected in direct response to alcohol consumption in body fluids, and can be isolated and purified. Simple, economical, and reproducible assay methods, such as a spot assay and an ascending or thin layer chromatography assay, provide reliable, objective, and sensitive methods for detecting and monitoring a chronic alcoholic condition. Both the presence of the alcohol-specific ethyl glucuronide and a substantial increase in its levels are indicative of chronic alcoholism.Type: GrantFiled: May 27, 1994Date of Patent: May 5, 1998Assignee: Research Foundation for Mental Hygiene, Inc.Inventors: Raju K. Pullarkat, Premila S. Pullarkat, Simhachalam Raguthu
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Patent number: 5726061Abstract: Methods of screening for colorectal cancer by measuring levels of HC gp-39 are provided. Methods of monitoring patients with colorectal cancer are also provided. In addition, kits for detection of HC gp-39 useful in screening for and monitoring of colorectal cancer in a patient are provided.Type: GrantFiled: October 8, 1996Date of Patent: March 10, 1998Assignee: SmithKline Beechum CorporationInventors: David Robbins, Robert B. Kirkpatrick, Stephen D. Holmes
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Patent number: 5710372Abstract: A simple, convenient method is provided for measuring the concentration of a constituent of an aqueous fluid composition (e.g. aqueous machining fluid composition) and for measuring total alkalinity thereof.Type: GrantFiled: December 17, 1996Date of Patent: January 20, 1998Assignee: Cincinnati Milacron Inc.Inventor: Giles J. P. Becket
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Patent number: 5683915Abstract: The present invention is a sample deposition device for depositing a plurality of samples onto a test media substrate, and a method of depositing a plurality of samples onto a planar test media substrate, such as a stationary phase chromatographic medium, agar or tissue. In broadest terms, the device of the present invention comprises (a) a substantially planar support portion defining a plane; and (b) an array of substantially planar absorbent portions releasably connected to the support portion, the absorbent portions aligned substantially parallel to said plane. The absorbent portions may be impregnated with a test sample before use. The present invention also includes a method of depositing one or more samples onto a test media substrate.Type: GrantFiled: October 31, 1995Date of Patent: November 4, 1997Assignee: Isolab, Inc.Inventors: Jeffrey A. Black, Vincent J. Greczanik, Michael E. Jackson
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Patent number: 5677190Abstract: An improved measurement cell and circuit for measuring the electrical characteristics of a liquid sample during exposure to radiation includes a glass cell formed of a main tube extending generally parallel to an elongated lamp emitting the radiation, and a pair of electrodes disposed axially within the sample tube. In the preferred embodiment, the ends of the cell are formed of glass twinbore tubing fused to the glass main tube, the bores retaining ends of the electrodes. Inlet and outlet tubes and a temperature sensing well may also be fused to the main tube. In an implementation for measurement of TOC in water by oxidation to CO.sub.2, the source comprises a low pressure mercury vapor lamp, and the electrodes are formed of solid Ti with catalytic TiO.sub.2 surfaces.Type: GrantFiled: December 14, 1994Date of Patent: October 14, 1997Assignee: Anatel CorporationInventors: Paul C. Melanson, Robert L. Garvin, Lawrence K. de Martin, David A. Pane, James A. Valdez
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Patent number: 5606116Abstract: A testing system for urinalysis is provided comprising a support member which carries a developing phase, a reagent carrying means, a urine applying section, and a urine-reagent developing area. In order to prevent inaccurate test results which may result from splashing the sample onto the device, a water-repellent agent is coated onto both sides of the reagent phase and the urine-reagent developing phase, as well as onto both sides of the support member where the reagent phase and the urine-reagent developing phase are provided. The water repellent agent is solidified, and a transparent resin film is attached to the upper surfaces of the reagent phase and the urine-reagent developing phase. In another embodiment, instead of the combination of a water repellent agent and a transparent resin film attached to the upper surfaces of the reagent phase and the urine-reagent developing phase, the device is enclosed in an envelope-like or cylindrical transparent resin film or container.Type: GrantFiled: October 30, 1995Date of Patent: February 25, 1997Assignee: Kabushiki Kaisha Nippon GeneInventors: Yuko Yoneda, Toshiyuki Itooka
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Patent number: 5516644Abstract: A specific binding assay process which is useful for quick qualitative or quantitative measurement and which can be used for various purposes, and a specific binding assay device suitable for the practice of the process,in which a substance to be assayed in a liquid test sample is determined qualitatively or quantitatively by developing the substance in a liquid test sample in a matrix, which comprises the steps of, for example:allowing the substance to be assayed to react with a specific binding substance which has specific affinity for the substance to be assayed;allowing a signal substance generator (a substance which competes with the substance to be assayed for the specific binding substance and which generates a signal) to chance its distribution in the matrix in response to the reaction of the above step, and;detecting the resulting distributional changes at a detection means as changes of signals which are rate-limited by the mass transfer of a signal substance generated from the signal substance geneType: GrantFiled: May 16, 1994Date of Patent: May 14, 1996Assignee: Mochida Pharmaceutical Co., Ltd.Inventors: Tadakazu Yamauchi, Toshinori Kanamori, Masahiro Nobuhara
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Patent number: 5470703Abstract: The present invention relates to a method for peptide C-terminal fragment sequence analysis, in which the fragment collection is carried out on an allylamine group-derivatized polymer membrane or on allylamine group-derivatized glass fiber filter paper; the collected C-terminal fragment is immobilized thereon using a water-soluble carbodiimide etc.; and the obtained immobilized product is subjected directly to amino acid sequence analysis. The present invention also relates to an apparatus for collecting a peptide fragment. According to the method of the present invention, peptides which are rich in hydrophobic groups in their C-terminus and are therefore difficult to trap with polyvalent ion carriers, currently used in the gas-phase sequencer, can be completely analyzed up to their C-terminus. Also, amino acid sequence analysis can be made even when the amount of C-terminal fragments is very small.Type: GrantFiled: October 15, 1993Date of Patent: November 28, 1995Assignee: Shimadzu CorporationInventors: Kiyoshi Nokihara, Rintaro Yamamoto
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Patent number: 5453382Abstract: Solutions of analytes in concentrations too low for certain analytical techniques such as various capillary electrophoresis techniques are concentrated in a solid-phase adsorption medium by a technique which involves a pressure-gradient-driven adsorption of the analyte followed by a desorption of the analyte and its removal using electroosmotic bulk flow.Type: GrantFiled: December 17, 1992Date of Patent: September 26, 1995Assignee: Indiana University FoundationInventors: Milos V. Novotny, Takao Tsuda, Helena A. Soini
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Patent number: 5451507Abstract: Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present.Type: GrantFiled: May 10, 1994Date of Patent: September 19, 1995Assignee: Syntex (U.S.A.) Inc.Inventors: Carl N. Skold, Armen B. Shanafelt, Vartan Ghazarossian, Edwin F. Ullman
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Patent number: 5443989Abstract: The present invention provides an accurate, rapid and precise methodology for assessing maturity of the lungs in a fetus prior to birth, especially for premature fetuses having a gestation period of 37 weeks or less. The methodology quantitatively measures a specific phosphoglyceride, dipalmitoyl phosphatidyl choline, in samples of amniotic fluid for the assessment of fetal lung maturity. The process enzymatically cleaves such phophoglycerides and preferably detects the resulting diacylglycerols by HPTLC-reflectance spectrodensitometry.Type: GrantFiled: October 25, 1993Date of Patent: August 22, 1995Assignee: Beth Israel Hospital AssociationInventors: Juan G. Alvarez, Jack Ludmir
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Patent number: 5334513Abstract: Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present.Type: GrantFiled: September 3, 1992Date of Patent: August 2, 1994Assignee: Syntex (U.S.A.) Inc.Inventors: Carl N. Skold, Armen B. Shanafelt, Vartan Ghazarossian, Edwin F. Ullman
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Patent number: 5310682Abstract: Compounds containing .alpha.-discarbonyl groups are rendered capable of fluorescent emission by conjugation with carboxylic acid-terminated ortho-diaminoaryl reagents. The carboxylic acid groups serve as ionizable moieties which facilitate the conjugation reaction, and are retained in the conjugation product to enable, or enhance the ability of, the conjugated compounds to be separated in chromatographic and electromigration separation processes, notably electrophoresis. Ortho-dinitro derivatives of the reagents are also disclosed, these derivatives offering the advantage of stability as well as the ability to be used directly in the reaction in the presence of a reducing agent.Type: GrantFiled: June 17, 1992Date of Patent: May 10, 1994Assignee: Indiana University FoundationInventors: Milos V. Novotny, Osamu Shirota, Donald Wiesler
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Patent number: 5306645Abstract: Methods of conducting Thin Layer Chromatography (TLC) involving using a TLC plate having sample spots and including no support material, placing the TLC plate on a porous substrate, and allowing solvent to move upwardly from the porous substrate toward the TLC plate by capillary action to be vaporized from the surface of the TLC plate, thereby concentrating the sample spots developed in the thin layer on the surface of the TLC plate.Type: GrantFiled: January 30, 1991Date of Patent: April 26, 1994Assignee: Shimadzu CorporationInventors: Hiroshi Yamamoto, Katsuhiko Ichimura, Kenji Nakamura, Tomiyuki Maeda, Takahiro Tajima, Kiyoshi Wada
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Patent number: 5302351Abstract: Payload customers for the Space Shuttle have recently expressed concerns about the possibility of their payloads at an adjacent pad being contaminated by plume effluents from a shuttle at an active pad as they await launch on an inactive pad. As part of a study to satisfy such concerns a ring of inexpensive dosimeters was deployed around the active pad at the inter-pad distance. However, following a launch, dosimeters cannot be read for several hours after the exposure. As a consequence factors such as different substrates, solvent systems, and possible volatilization of HCl from the badges were studied. This observation led to the length of stain (LOS) dosimeters of this invention. Commercial passive LOS dosimeters are sensitive only to the extent of being capable of sensing 2 ppm to 20 ppm if the exposure is 8 hours. To map and quantitate the HCl generated by Shuttle launches, and in the atmosphere within a radius of 1.Type: GrantFiled: November 16, 1992Date of Patent: April 12, 1994Assignee: The United States of America as represented by the Administrator of the National Aeronautics and Space AdministrationInventor: Dale E. Lueck
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Patent number: 5171528Abstract: The specification discloses a fecal occult blood test device capable of determining whether the blood found during the test originated in the upper or lower gastrointestinal track. A fecal sample is applied to a test medium charged to be differentially attractive to blood components originating in the upper and lower gastrointestinal track respectively. A solvent is applied to the test specimen to cause differential migration of the blood components and an indicator is then applied to indicate the presence of the blood components, if any.Type: GrantFiled: August 5, 1991Date of Patent: December 15, 1992Inventors: Stephen C. Wardlaw, Robert A. Levine
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Patent number: 5164294Abstract: Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present.Type: GrantFiled: July 7, 1989Date of Patent: November 17, 1992Assignee: Syntex (U.S.A.) Inc.Inventors: Carl N. Skold, Armen B. Shanafelt, Vartan Ghazarossian, Edwin F. Ullman