Carrier Is Synthetic Resin Patents (Class 436/531)
  • Publication number: 20040259272
    Abstract: The invention concerns a fixed support for immobilizing biomolecules, said support being covered, at least in some parts, with a polymer. The invention is characterized in that the polymer is an epoxy resin with functionality ranging between 4 and 15. The invention also concerns a method for making such a support.
    Type: Application
    Filed: March 24, 2004
    Publication date: December 23, 2004
    Inventor: Claudia Preininger
  • Patent number: 6833276
    Abstract: Sensors for determining the presence and concentration of bio-molecules in a biological sample are provided in the form of polymer brushes, which comprise a substrate having a surface that is modified with a water-dispersible or water-soluble polymer segment having functional groups that bind probes. The method of synthesis of such sensors preferably includes use of controlled free radical polymerization techniques, and in particular the use of an iniferter initiator, which allows for controlled architecture polymers to modify the surface of the substrate. In this manner functional groups in the polymer chain are removed from the surface, which allows for solution chemistry to be more realistically reproduced with the benefits of a solid bound probe.
    Type: Grant
    Filed: July 24, 2001
    Date of Patent: December 21, 2004
    Assignee: Symyx Technologies, Inc.
    Inventors: Gerrit Klaerner, Ralph B. Nielsen, Paul Mansky, Didier Benoit, Dominique Charmot, Bernd Jandeleit, Laura T. Mazzola
  • Patent number: 6828159
    Abstract: The invention relates to the use of carbopyronine compounds of general formula (I) as marker groups in methods for detecting analytes. The invention also relates to novel carbopyronine compounds and to a method for producing same.
    Type: Grant
    Filed: October 26, 2001
    Date of Patent: December 7, 2004
    Inventors: Karl-Heinz Drexhage, Jutta Arden-Jacob, Jörg Frantzeskos, Alexander Zilles
  • Patent number: 6825032
    Abstract: A high capacity assay platform capable of binding target molecules includes a substrate and a polymer matrix attached to the substrate. The polymer matrix comprises a plurality of polymer molecules where at least some of the polymer molecules are covalently attached directly to the substrate and at least some of which molecules are crosslinked to other polymer molecules. Some of the polymer molecules have at least one binding ligand covalently attached thereto, and the density of the polymer matrix on the substrate is at least 2 &mgr;g/cm2.
    Type: Grant
    Filed: May 14, 2001
    Date of Patent: November 30, 2004
    Assignee: Sigma-Aldrich Co.
    Inventors: John Dapron, William Karl Kappel, Handong Li
  • Patent number: 6824997
    Abstract: A process is disclosed for obtaining a C-polysaccharide cell wall antigen containing not more than about 10% protein from Streptococcus pneumoniae bacteria. The antigen thus obtained is conjugated to a spacer molecule, and the free end of the latter is then conjugated to a chromatographic affinity column. The column is then utilized to purify raw antibodies to S. pneumonia bacteria, thereby producing antigen-specific antibodies. A portion of such antibodies is conjugated to a labeling agent which displays a visible color change upon reaction of the antibodies with their antigenic binding partner and embedded in a first zone of an immunochromatographic assay device. Another portion of such antibodies is bound to the reaction zone of the device which has a view window.
    Type: Grant
    Filed: September 16, 1999
    Date of Patent: November 30, 2004
    Assignee: BINAX, Inc.
    Inventors: Norman James Moore, Mary Kathleen Fent, Vladimir Andrei Koulchin, Elena Valentin Molokova
  • Patent number: 6821529
    Abstract: The present invention provides biotechnologically useful oligo(ethylene glycol)-terminated 1,2-dithiolane compositions and conjugates of these compositions with biological or non-biological receptor, ligand, sequestering, or reporter moieties. The invention also provides methods for the preparation of these compositions. Further, the invention provides self-assembled monolayer (SAM) compositions on a metal and methods for their preparation.
    Type: Grant
    Filed: September 5, 2001
    Date of Patent: November 23, 2004
    Inventor: Deanna Jean Nelson
  • Publication number: 20040229382
    Abstract: This invention relates to the characterization of mixtures. More specifically, this invention provides tools and methodologies for characterizing a mixture by detecting defined and undefined structures which form when the substances of the mixture contact a configuration of immobilized polymers.
    Type: Application
    Filed: October 19, 2001
    Publication date: November 18, 2004
    Inventors: Ronald Breaker, Yung-Chi Cheng
  • Patent number: 6818417
    Abstract: A method for locating hidden microorganism contaminated surfaces in industrial water systems is described and claimed. The method works by applying a solution or dispersion of a fluorogenic reagent in water to the hidden water contact surfaces of said industrial water system and allowing said fluorogenic reagent to react with any hidden microorganisms present, wherein said fluorogenic reagent is selected from the group of fluorogenic reagents that are known to react with microorganisms such that a fluorescent signal of said fluorogenic reagent is detectable in such a way as to make the detection of the fluorescent signal indicate that there are hidden microorganisms present on the water contact surfaces of the equipment and piping.
    Type: Grant
    Filed: August 6, 2002
    Date of Patent: November 16, 2004
    Assignee: Nalco Company
    Inventors: Andrew J. Cooper, Michael V. Enzien, Steven R. Hatch, Bosco P. Ho, May M. Wu
  • Patent number: 6818455
    Abstract: An analytical test device useful for example in pregnancy testing, comprises a hollow casing (500) constructed of moisture-impervious solid material, such as; plastics materials, containing a dry porous carrier (510) which communicates indirectly with the exterior of the casing via a bibulous sample receiving member (506) which protrudes from the casing such that a liquid test sample can be applied to the receiving member and permeate therefrom to the porous carrier, the carrier containing in a first zone a labelled specific binding reagent is freely mobile within the porous carrier when in the moist state, and in a second zone spatially distinct from the first zone unlabelled specific binding reagent for the same analyte which unlabelled reagent is permanently immobilised on the carrier material and is therefore not mobile in the moist state, the two zones being arranged such that liquid sample applied to the porous carrier can permeate via the first zone into the second zone, and the device incorporating me
    Type: Grant
    Filed: February 28, 2001
    Date of Patent: November 16, 2004
    Assignee: Inverness Medical Switzerland GmbH
    Inventors: Keith May, Michael Evans Prior, Ian Richards
  • Patent number: 6815217
    Abstract: A solid phase with at least one test area is described which contains reagents for the detection of at least one analyte in a sample, wherein the solid phase additionally comprises at least one control area for the detection of interfering reactions.
    Type: Grant
    Filed: January 21, 2000
    Date of Patent: November 9, 2004
    Assignee: Roche Diagnostics GmbH
    Inventors: Johann Karl, Helmut Lenz, Friedemann Krause, Peter Finckh, Hans Hornauer, Johann Berger
  • Patent number: 6808908
    Abstract: The invention relates to porous polymeric materials, methods of making them, and applications in medical devices. A specific embodiment of the invention encompasses a material comprising a porous polyolefin substrate containing inclusions of a material to which chemical or biological moieties are attached directly or via a spacer.
    Type: Grant
    Filed: May 30, 2001
    Date of Patent: October 26, 2004
    Assignee: Porex Technologies Corporation
    Inventors: Li Yao, George Warren Greene, IV, Guoqiang Mao, Xingguo Li
  • Patent number: 6797522
    Abstract: The invention is directed to synthetic receptor(s) which comprises a polyfunctional organic template covalently linked to two or more oligomers which may independently be the same or different and may independently be straight chain, cyclic or branched. The template may be linked to an identifier which uniquely defines the synthetic receptor. The identifier is a stable chemical molecule or a plurality of stable chemical molecules distinguishable and detectable to picomolar levels or may be an oligonucleotide. In a preferred embodiment, the template is covalently linked to a solid support which is linked to an identifier. In addition, the invention includes methods of preparing synthetic receptors and synthetic receptor libraries. The synthetic library may be linked with identifiers such that the library comprises a plurality of different synthetic receptor members.
    Type: Grant
    Filed: March 12, 1998
    Date of Patent: September 28, 2004
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: W. Clark Still, Ge Li, Helma Wennemers
  • Patent number: 6790965
    Abstract: Combinatorial libraries are disclosed which are represented by Formula I: (T′—L)q—Ŝ—C(O)—L′—II′  I wherein: Ŝ is a solid support; T′—L— is an identifier residue; and —L′—II′ is a ligand/linker residue. These libraries contain dihydrobenzopyrans of the formula: which interact (i.e., as agonists or antagonists) with &agr; adrenergic receptors, dopamine receptors, &dgr;-opiate receptors, and K+ channels and are inhibitors of carbonic anhydrase isozymes. They are useful in the treatement of ocular diseases such as glaucoma.
    Type: Grant
    Filed: September 8, 1999
    Date of Patent: September 14, 2004
    Assignee: Pharmacopeia Drug Discovery, Inc.
    Inventors: John J. Baldwin, John C. Reader, Lawrence W. Dillard, Ge Li, Wenguang Zeng
  • Publication number: 20040175843
    Abstract: An encoded patterned microbead of polymeric material, with an associated geometry, capable of linking to a ligand molecule, processes for fabricating shaped and patterned microbeads, a reader to read the patterned microbead, and methods to produce and read the shaped and patterned microbead are disclosed. A unique identifier is written to the encoded patterned microbead and the encoded patterned microbead is given an identifying shape according to one of several well-known techniques. A reader of the present invention, as well as conventional readers, read the shaped, encoded, patterned microbeads.
    Type: Application
    Filed: September 24, 2003
    Publication date: September 9, 2004
    Inventors: Daniel B. Roitman, Annette Grot
  • Publication number: 20040175842
    Abstract: An encoded patterned microbead of polymeric material capable of linking to a ligand molecule, a process for fabricating patterned microbeads, a reader to read the patterned microbead, and methods to produce the patterned microbead are disclosed. A unique identifier is written to the encoded patterned microbead according to one of several well-known techniques. A reader of the present invention, as well as conventional readers, read the encoded patterned microbeads.
    Type: Application
    Filed: March 4, 2003
    Publication date: September 9, 2004
    Inventors: Daniel B. Roitman, Kevin P. Killeen, Annette C. Grot
  • Patent number: 6783962
    Abstract: The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 &mgr;m, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.
    Type: Grant
    Filed: November 20, 2001
    Date of Patent: August 31, 2004
    Assignee: UpFront Chromatography
    Inventors: Morten Aae Olander, Allan Otto Fog Lihme, Timothy John Hobley, Marcos Simon, Irini Theodossiou, Owen Robert Tyrynis Thomas
  • Patent number: 6783947
    Abstract: Alpha-haloketones are useful alkylating agents for coupling to sulfhydryl-containing biomolecules. However, they react spontaneously with water, alkali and organic bases and therefore cannot be stored for extended periods of time in aqueous solutions, particularly in the presence of proteins at physiological pH. The present invention provides novel solutions to these problems, however, as novel compounds and compositions comprising protected haloketones are disclosed herein. Methods of preparing and using protected haloketones which are useful in a variety of applications—e.g., in assays and conjugation reactions—are also disclosed herein.
    Type: Grant
    Filed: September 9, 1999
    Date of Patent: August 31, 2004
    Assignee: Dade Behring Marburg GmbH
    Inventors: Steve de Keczer, Yen Ping Liu, Dariush Davalian, Nurith Kurn, Edwin F. Ullman
  • Patent number: 6770487
    Abstract: Diagnostic test devices, including diagnostic strip tests, are provided in which identifying information and the test result are machine-readable. Also provided are methods for obtaining identifying information and test results from the diagnostic test devices.
    Type: Grant
    Filed: May 1, 2001
    Date of Patent: August 3, 2004
    Assignee: Ischemia Technologies, Inc.
    Inventor: Peter A. Crosby
  • Patent number: 6770447
    Abstract: The present invention involves an optical assay device and method of use for the detection of an analyte of interest in a sample that conveniently allows control of the flow characteristics of the sample through the device without significant user intervention. The optical assay device includes a base having an absorbent material, and a member having an optically active test stack that is rotatably coupled to the base for rotation between a lowered position and a raised position. In the lowered position, the optically active test stack contacts the absorbent material for drawing the sample through the surface. In the raised position, the optically active test stack does not contact the absorbent material.
    Type: Grant
    Filed: July 12, 2001
    Date of Patent: August 3, 2004
    Assignee: Thermo Biostar, Inc.
    Inventors: James E. Maynard, Mark A. Crosby, Alan J. Fujii
  • Publication number: 20040132218
    Abstract: A biochip and apparatus is disclosed for performing biological assays in a self-contained microfluidic platform. The disposable biochip for multi-step reactions comprises a body structure with a plurality of reagent cavities and reaction wells connected via microfluidic channels; the reagent cavities with reagent sealing means for storing a plurality of reagents; the reagent sealing means being breakable and allowing a sequence of reagents to be released into microfluidic channel and reaction well; and the reaction well allowing multi-step reactions to occur by sequentially removing away the residual reagents. The analysis apparatus can rapidly, automatically, sensitively, and simultaneously detect and identify multiple analytes or multiple samples in a very small quantity.
    Type: Application
    Filed: January 8, 2003
    Publication date: July 8, 2004
    Inventor: Winston Z. Ho
  • Patent number: 6756354
    Abstract: The present invention provides biotechnologically useful oligo(ethylene glycol)-terminated 1,2-dithiolane compositions and conjugates of these compositions with biological or non-biological receptor, ligand, sequestering, or reporter moieties to provide physiologically active therapeutic compositions. The invention also provides methods for the preparation of these compositions. Further, the invention provides self-assembled monolayer (SAM) compositions on a metal and methods for their preparation.
    Type: Grant
    Filed: April 3, 2002
    Date of Patent: June 29, 2004
    Inventor: Deanna Jean Nelson
  • Patent number: 6756234
    Abstract: It has been found that casein and salts of casein are useful as replacements for, or in addition to, BSA as materials for coating solid phases, particularly magnetic particles, used for immunoassays and other binding assays for separation of the desired analyte. By using casein, immunoassays having improved stability and few discordant samples have been developed. Casein used at a concentration of 0.05-4.0 grams per gram of paramagnetic particle (optimally approximately 0.78-1.2 grams of casein per gram of magnetic particle) has been found to confer this benefit. In addition, a process for coating solid phases has been invented, said process comprising the mixing of casein with magnetic particles at 30-60° C. for 5-180 hours, said process resulting in casein-coated paramagnetic particles which either (1) already have combined therewith active ingredients needed in the binding assay or (2) are capable of reacting with active ingredients needed in the binding assay.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: June 29, 2004
    Assignee: Bayer Corporation
    Inventors: Wei-Chao Ni, Daniel W. Eustace, Steve Chin-Shen Chang
  • Patent number: 6756233
    Abstract: A method for measuring free ligands in biological fluids in the presence of bound ligand and endogenous binding proteins, without disturbing the equilibrium between the free ligand and the protein-bound ligand, comprised of the following steps: (a) incubating a sample of biological fluid with (i) a ligand analog tracer which, due to its chemical structure, does not bind to some of the endogenous binding proteins, (ii) a specific ligand binder and (iii) specific chemical inhibitor reagents that alone or in combination inhibit the binding of the ligand analog tracer to other endogenous binding proteins; (b) separating the ligand analog tracer bound to the specific binder from unbound tracer; and (c) comparing the bound fraction in said sample to the bound fraction of a given set of known free ligand calibrators to determine the concentration of free ligand in said biological fluid.
    Type: Grant
    Filed: March 9, 1998
    Date of Patent: June 29, 2004
    Inventor: A. Said El Shami
  • Patent number: 6753191
    Abstract: A novel method is disclosed wherein polymerized crystalline colloidal array (PCCA) chemical sensing materials are used to detect the presence of certain chemical species in high ionic strength solutions, such as bodily fluids. The PCCA sensors consist of a mesoscopically periodic array of colloidal particles polymerized into a hydrogel. The array of colloidal particles diffracts light in the visible spectral region due to the periodic spacing of the particles. The PCCA materials also contain molecular recognition components that bind to the chemical species being detected. The binding or the chelation of the chemical species of interest results in a Donnan potential that swells the hydrogel and in turn red shifts the diffracted light proportionately to the concentration of the chemical species. However, no significant red shift response may occur for PCCA chemical sensors in high ionic strength solutions containing chemical species of interest.
    Type: Grant
    Filed: September 17, 2001
    Date of Patent: June 22, 2004
    Assignee: University of Pittsburgh
    Inventors: Sanford A. Asher, Chad E Reese
  • Patent number: 6743585
    Abstract: Methods are disclosed for conjugating one moiety to another moiety. In the method the moieties are reacted with one another in a protic solvent. Reaction between the moieties and the protic solvent during the conjugating is negligible or reversible. A stable bond is formed between the moieties to produce a product that is not subject to &bgr;-elimination at elevated pH. Usually, one of the moieties comprises an unsaturation between two carbon atoms. One of the carbon atoms is or becomes an electrophile during the conjugating. The other of the moieties comprises a functionality reactive with the electrophile carbon atom to form a product that comprises the unsaturation. Compounds comprising both of the moieties as well as precursor molecules are also disclosed. Methods are also disclosed for determining an analyte in a sample employing compounds as described above.
    Type: Grant
    Filed: October 17, 2001
    Date of Patent: June 1, 2004
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Joel Myerson, Geraldine Fulcrand, Diane D. Ilsley
  • Patent number: 6737279
    Abstract: The present invention provides a method of detecting a biological agent including contacting a sample with a sensor including a polymer system capable of having an alterable measurable property from the group of luminescence, anisotropy, redox potential and uv/vis absorption, the polymer system including an ionic conjugated polymer and an electronically inert polyelectrolyte having a biological agent recognition element bound thereto, the electronically inert polyelectrolyte adapted for undergoing a conformational structural change upon exposure to a biological agent having affinity for binding to the recognition element bound to the electronically inert polyelectrolyte, and, detecting the detectable change in the alterable measurable property. A chemical moiety being the reaction product of (i) a polyelectrolyte monomer and (ii) a biological agent recognition element-substituted polyelectrolyte monomer is also provided.
    Type: Grant
    Filed: March 28, 2001
    Date of Patent: May 18, 2004
    Assignee: The Regents of the University of California
    Inventor: Liaohai Chen
  • Patent number: 6737280
    Abstract: A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having structure (I) wherein Y1 and Y2 are individually hydroxyl, amino, imminium, or oxygen, R1-R8 are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinatios thereof, and R9 is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having general structure (II) where substituents R3-R7 correspond to like-referenced substituents in the structure of described above, and Y2 is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates. In yet another aspect, the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides.
    Type: Grant
    Filed: November 26, 2001
    Date of Patent: May 18, 2004
    Assignee: Karl-Heinz Drexhage
    Inventors: Karl-Heinz Drexhage, Jutta Arden-Jacob, Norbert Kemnitzer
  • Patent number: 6730905
    Abstract: There is provided a probe for a scanning probe microscope, comprising: a proximal end; and a distal tip portion, wherein the distal tip portion has a tip surface which faces a fixed sample, and at least one monolayer is formed at least on the tip surface, and a molecule having a chemical sensor function or catalytic function is placed in or on an outermost monolayer above the tip surface. There is provided a probe for a scanning probe microscope, comprising: a cover layer containing an electrically conductive polymer; and a catalyst in the cover layer, the catalyst being selected from a group consisting of inorganic catalysts and organic catalysts. There are provided a scanning probe microscope equipped with the above probe, and a molecule processing method using such a scanning probe microscope.
    Type: Grant
    Filed: May 17, 2002
    Date of Patent: May 4, 2004
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Tohru Nakagawa, Tetsuo Yukimasa
  • Patent number: 6720192
    Abstract: A method for assaying an analyte in a sample. The method comprising the steps of a) contacting the sample with material comprising a receptor which is present in a liposome and which liposome comprises a detectable functionality, said contact occurring under conditions resulting in binding of the receptor to analyte if present before or concomitant with step b, wherein step b) consists of contacting the sample with an immobilised ligand for the receptor said contact occurring under conditions resulting in binding of the receptor to the ligand, with steps a and b being followed by c) separating the resulting immobilised ligand-receptor fraction and the receptor fraction present in solution and d) assaying the detectable functionality of the receptor in a fraction from step c) in a manner known per se for its detection.
    Type: Grant
    Filed: May 9, 2001
    Date of Patent: April 13, 2004
    Assignee: Merska B.V.
    Inventors: Gerhard Theodoor Viel, Kornelis Ensing
  • Patent number: 6716639
    Abstract: The invention relates to an immunoassay apparatus, comprising a dry, porous substrate having at least two sections (1, 2), communicating with one another in the moist state by capillary action, wherein the first section (1) has a marked detection reagent for the substance to be detected and the second section (2) has an immobilized binder reagent for the substance to be detected, and wherein the reaction product comprising the substance to be detected and the marked detection reagent migrates by capillary action from the first section into the second section in the moist state, and is characterized in that the marked detection reagent is a dye-carrying reagent for nicotine or cotinine.
    Type: Grant
    Filed: November 9, 1998
    Date of Patent: April 6, 2004
    Assignee: ulti med Products (Deutschland) GmbH
    Inventor: Matthias Werner Engel
  • Patent number: 6716641
    Abstract: The invention relates to a new dipstick assay for detecting and quantifying the content of an target analyte in a sample. The assay is particularly useful for example in the diagnosis and monitoring of alcoholism by the detection of asialo transferrin or carbohydrate free transferrin (CFT). Thus, provided is a dipstick for determining the content of a target analyte variant in a mixture of analyte variants in a sample, comprising: a) a sample application zone, b) a screening zone having an immobilised binding ligand having a binding affinity for a non-target analyte variant or varinats, c) a conjugate zone comprising a detector reagent, d) a reading zone for detection of said analyte.
    Type: Grant
    Filed: September 14, 2001
    Date of Patent: April 6, 2004
    Assignee: Axis-Shield ASA
    Inventor: Erling Sundrehagen
  • Patent number: 6713271
    Abstract: Novel magnetic assay methods and systems. According to a preferred embodiment, a chromatographic medium, which preferably comprises a test strip, is provided that is designed to be contacted with a test solution having activated magnetic particles such that the solution flows bilaterally thereacross. A magnetic field, generated by a magnet or electromagnet, is selectively applied to the medium which causes the charged particles to become substantially bound at a site on the medium specified by the position off the magnet, to thus form a captured line or zone. In one preferred embodiment, the magnetic field is applied at the site on the medium at which the test solution is contacted. The degree of magnetic force applied to the membrane may be selectively adjusted to vary the width or surface area of the capture line or zone.
    Type: Grant
    Filed: September 25, 2000
    Date of Patent: March 30, 2004
    Assignee: Wavesense, LLC
    Inventor: Christopher Feistel
  • Patent number: 6713298
    Abstract: A system for the rapid characterization of multi-analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member into which a plurality of cavities may be formed. A series of chemically sensitive particles are, in one embodiment positioned within the cavities. The particles may be configured to produce a signal when a receptor coupled to the particle interacts with the analyte. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized.
    Type: Grant
    Filed: January 31, 2001
    Date of Patent: March 30, 2004
    Assignee: Board of Regents, The University of Texas System
    Inventors: John T. McDevitt, Eric V. Anslyn, Jason B. Shear, Dean P. Neikirk
  • Patent number: 6703205
    Abstract: Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.
    Type: Grant
    Filed: March 19, 2002
    Date of Patent: March 9, 2004
    Assignee: Caliper Technologies Corp.
    Inventors: Anne R. Kopf-Sill, Andrea W. Chow, Claudia B. Cohen, Steven A. Sundberg, John Wallace Parce
  • Patent number: 6699667
    Abstract: Disclosed is a sensor for sensing the presence of an analyte component without relying on redox mediators. This sensor includes (a) a plurality of conductive polymer strands each having at least a first end and a second end and each aligned in a substantially common orientation; (b) a plurality of molecular recognition headgroups having an affinity for the analyte component and being attached to the first ends of the conductive polymer strands; and (c) an electrode substrate attached to the conductive polymer strands at the second ends. The electrode substrate is capable of reporting to an electronic circuit reception of mobile charge carriers (electrons or holes) from the conductive polymer strands. The electrode substrate may be a photovoltaic diode.
    Type: Grant
    Filed: September 20, 2001
    Date of Patent: March 2, 2004
    Assignee: KeenSense, Inc.
    Inventor: Randy E. Keen
  • Patent number: 6696265
    Abstract: The present invention relates to the substantial elimination of errors attributable to carryover microspheres, doublets, or misclassification of microsphere subsets. The present invention is based upon passing a sufficient minimum number microspheres through the flow analyzer during an assay run.
    Type: Grant
    Filed: August 17, 2000
    Date of Patent: February 24, 2004
    Assignee: Luminex Corporation
    Inventor: Michael D. Spain
  • Patent number: 6692973
    Abstract: The present invention relates to bioassay materials useful for the detection of toxic substances and, more particularly, to packaging materials for food and other products, along with methods for their manufacture and use. The invention provides a unique composite material capable of detecting and identifying multiple biological materials within a single package. The biological material identification system is designed for incorporation into existing types of flexible packaging material such as polyvinylchloride or polyolefin films, and its introduction into the existing packaging infrastructure will require little or no change to present systems or procedures.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: February 17, 2004
    Assignee: Toxin Alert, Inc.
    Inventors: William T. Bodenhamer, George Jackowski, Eric Davies
  • Patent number: 6686170
    Abstract: The present invention relates to an improved immunoassay device for confirming the validity of a test result showing either the presence or absence of an analyte in a patient sample. In the improved device, control reagents are provided in the device which directly mimic the reaction of the sample analyte with the test reagents of the device. The device thus allows the user to verify the efficacy of the test reagents at all stages of interaction with the sample analyte.
    Type: Grant
    Filed: August 17, 1994
    Date of Patent: February 3, 2004
    Assignee: Abbott Laboratories
    Inventors: Richard T. Flanders, Vincent A. Varitek, Jr.
  • Patent number: 6686161
    Abstract: The present invention provides solid supports (e.g., glass) and polymer hydrogels (particularly polymer hydrogel arrays present on a solid support) comprising one or more reactive sites for the attachment of biomolecules, as well as biomolecules comprising one or more reactive sites for attachment to solid supports and polymer hydrogels. The invention further provides novel compositions and methods for the preparation of biomolecules, solid supports, and polymer hydrogels comprising reactive sites. The invention also provides for preparation of crosslinked solid supports, polymer hydrogels, and hydrogel arrays, wherein one or more biomolecules is attached by means of the reactive sites in a photocycloaddition reaction. Advantageously, according to the invention, crosslinking of the hydrogel and attachment of biomolecules can be done in a single step.
    Type: Grant
    Filed: October 11, 2001
    Date of Patent: February 3, 2004
    Assignee: Amersham Biosciences AB
    Inventors: Travis Johnson, John McGowen, Allyson Beuhler, Charles Kimball Brush, Robert Emil Lajos
  • Patent number: 6686169
    Abstract: Reagents and methods for the detection of Staphylococcus aureus are provided. The reagents contain an antibody that binds to a capsular polysaccharide of type 5 of Staphylococcus aureus, and can be used in methods for detection of oxacillin resistant Staphylococcus aureus that escapes detection by agglutination in the presence of fibrinogen and antibodies directed against protein A of Staphylococcus.
    Type: Grant
    Filed: August 7, 2002
    Date of Patent: February 3, 2004
    Assignee: Institut Pasteur
    Inventors: Jean-Michel Fournier, Alain Boutonnier
  • Patent number: 6680210
    Abstract: The present invention provides imprint compositions useful for capturing, isolating, detecting and/or quantifying macromolecules in a sample, methods of making and using the same. Generally, the imprint compositions comprise a matrix material defining an imprint of a template molecule, and the template molecule typically corresponds to a portion of a macromolecule of interest.
    Type: Grant
    Filed: June 28, 2001
    Date of Patent: January 20, 2004
    Assignee: Aspira Biosystems, Inc.
    Inventor: Chin-Shiou Huang
  • Publication number: 20040009500
    Abstract: Items described herein possess an activated surface for immobilisation of bioorganic macromolecules, i.e.
    Type: Application
    Filed: January 31, 2003
    Publication date: January 15, 2004
    Applicant: chimera biotec GmbH
    Inventors: Rudiger Benters, Christof M. Niemeyer, Dieter Wohrle
  • Patent number: 6673561
    Abstract: Thrombotic or thromboembolic disease is detected or monitored by determining the presence or amount B in a urine sample.
    Type: Grant
    Filed: June 30, 2000
    Date of Patent: January 6, 2004
    Assignee: Regents of the University of California
    Inventor: Timothy A. Morris
  • Patent number: 6670298
    Abstract: The present invention relates, inter alia, to methodologies for the synthesis, screening and characterization of organometallic compounds and catalysts (e.g., homogeneous catalysts). The methods of the present invention provide for the combinatorial synthesis, screening and characterization of libraries of supported and unsupported organometallic compounds and catalysts (e.g., homogeneous catalysts). The methods of the present invention can be applied to the preparation and screening of large numbers of organometallic compounds which can be used not only as catalysts (e.g., homogeneous catalysts), but also as additives and therapeutic agents.
    Type: Grant
    Filed: June 21, 1999
    Date of Patent: December 30, 2003
    Assignee: Symyx Technologies, Inc.
    Inventors: W. Henry Weinberg, Eric McFarland, Isy Goldwasser, Thomas Boussie, Howard Turner, Johannes A. M. Van Beek, Vince Murphy, Timothy Powers
  • Patent number: 6670199
    Abstract: The present invention is directed to a composition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfaces and hydrophobic coated surfaces. The composition is a biomolecule conjugated end-group activated polymer (EGAP). The biomolecule conjugated EGAP can be put to numerous uses including cell adhesion, cell growth, cell sorting, and other biological assays.
    Type: Grant
    Filed: September 4, 2001
    Date of Patent: December 30, 2003
    Assignee: University of Utah Research Foundation
    Inventors: Karin D. Caldwell, Patrick A. Tresco, Jennifer Neff
  • Patent number: 6667406
    Abstract: Disclosed are methods of synthesizing libraries of diverse complex hexahydroisoindole, hexahydroisooxyindole and octahydroisooxyquinoline compounds through the use of a facile intramolecular Diels Alder reaction. The invention is further directed to methods for synthesizing the libraries on solid supports. The invention is further directed to methods for identifying and isolating such hexahydroisoindole, hexahydroisooxyindole and octahydroisooxyquinoline compounds with useful and diverse activities from such libraries.
    Type: Grant
    Filed: March 7, 2000
    Date of Patent: December 23, 2003
    Assignee: Ortho-McNeil Pharmaceutical, Inc.
    Inventors: Sengen Sun, William V. Murray
  • Patent number: 6667180
    Abstract: This invention relates to a method of assaying pyrrole-containing biological compounds and chemical compositions that can be used in the method. The method involves contacting a biological compound with one of: a) a bound or bindable derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a detectable molecule which forms a complex with the reaction product; or b) a derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a bound binding agent specific to the biological compound in the reaction product; or c) a binding agent specific to the biological compound, followed by exposure to a derivatizing agent which forms a reaction product with the biological compound, and determining the amount of bound biological compound. There is also provided a method of preparing an antigen.
    Type: Grant
    Filed: October 3, 2001
    Date of Patent: December 23, 2003
    Assignee: Rowett Research Institute
    Inventors: Jeffrey D. Brady, Simon P. Robins
  • Patent number: 6660176
    Abstract: Small particles of polymeric material are produced by expansion of a mixture of monomers and a propellant. The size and shape of the particles can be precisely tailored by materials selection and expansion conditions. Particles of 10 nanometers to 100 microns can be produced. If monomers exhibiting solid state reactivity are utilized, the particles thus formed can be polymerized at any time after formation. The particles produced by this method can be molecularly imprinted by incorporating a template into the particle prior to fully curing the particle, in a manner which allows selective extraction of the template from the cured particle after formation without deformation of the imprint site. A two step polymerization process allows the particles to be deposited on and adhered to a wide variety of substrates without additional agents.
    Type: Grant
    Filed: January 24, 2002
    Date of Patent: December 9, 2003
    Assignee: Virginia Commonwealth University
    Inventors: Gary Tepper, Dmitry Pestov, Natalia Levit, Gary Wnek
  • Patent number: 6660484
    Abstract: The present invention relates to methods and compositions for the direct detection of analytes using observable spectral changes in biopolymeric systems. In particular, the present invention allows for the direct colorimetric detection of analytes using color changes that occur in glycopolythiophene polymer systems in response to selective binding of analytes.
    Type: Grant
    Filed: December 11, 2000
    Date of Patent: December 9, 2003
    Assignee: Regents of the University of California
    Inventors: Deborah J. Charych, Myung-Gi Baek
  • Patent number: 6660780
    Abstract: A method of making a molecularly imprinted porous structure makes use of a surfactant analog of the molecule to be imprinted that has the imprint molecule portion serving as the surfactant headgroup. The surfactant analog is allowed to self-assemble in a mixture to create at least one supramolecular structure having exposed imprint groups. The imprinted porous structure is formed by adding reactive monomers to the mixture and allowing the monomers to polymerize, with the supramolecular structure serving as a template. The resulting solid structure has a shape that is complementary to the shape of the supramolecular structure and has cavities that are the mirror image of the imprint group. Similarly, molecularly imprinted particles may be made by using the surfactant to create a water-in-oil microemulsion wherein the imprint groups are exposed to the water phase.
    Type: Grant
    Filed: November 27, 2002
    Date of Patent: December 9, 2003
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventors: Michael A. Markowitz, Paul E. Schoen, Bruce P. Gaber, Banahalli R. Ratna, Paul R. Kust, David C. Turner, Douglas S. Clark, Jonathan S. Dordick