Carrier Is Synthetic Resin Patents (Class 436/531)
  • Publication number: 20030220474
    Abstract: Conjugates of a biodegradable aliphatic polyester-based polymer with Tat49-57 peptide or a peptide chain containing the Tat49-57 peptide, and nanoparticles manufactured using the same. Intracellular permeability of the Tat49-57 peptide can be enhanced by exposing Tat peptide moieties to the surface of the nanoparticles.
    Type: Application
    Filed: June 28, 2002
    Publication date: November 27, 2003
    Applicant: PACIFIC CORPORATION
    Inventors: Ju Young Park, Yoon Sung Nam, Sang Hoon Han, Ih Seop Chang
  • Patent number: 6653153
    Abstract: A combinatorial library comprises a plurality of ligand-bearing support units, where the ligand-bearing support units comprise (a) a solid carrier, (b) one or more ligands covalently bound to the solid carrier, and (c) one or more encoding metal salts impregnated on the support unit, where the distribution of the cations of the encoding metal salts provides a code for identifying the ligand or ligands that are attached to the support units, and the cations have been stabilized against dissolution by treatment with one or more anions that form insoluble or poorly soluble salts of the cations.
    Type: Grant
    Filed: July 3, 2001
    Date of Patent: November 25, 2003
    Assignee: Merck & Co., Inc.
    Inventors: Yusheng Xiong, Kevin Chapman, Tiebang Wang
  • Patent number: 6653152
    Abstract: An optical assaying method and system having a movable sensor is described. In one aspect, the present invention is a sensing system having a rotating sensor disk coated with indicator dyes sensitized to a variety of substances. In this configuration the sensing system further includes a detector for sensing spectral changes in light received from one or more of the indicator dyes. In another aspect, the present invention is a sensing system having a surface plasmon resonance sensor disk having grooves extending radially from a center of the disk. In yet another aspect, the present invention is a sensing system including a diffraction anomaly sensor disk having a dielectric layer that varies in thickness. The present invention allows for construction of an inexpensive sensing system that is capable of easily detecting a variety of substances either in a sample or a surrounding environment.
    Type: Grant
    Filed: May 30, 2001
    Date of Patent: November 25, 2003
    Assignee: Imation Corp.
    Inventor: William A. Challener
  • Patent number: 6649403
    Abstract: A system for the rapid characterization of multi-analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member into which a plurality of cavities may be formed. A series of chemically sensitive particles are, in one embodiment positioned within the cavities. The particles may be configured to produce a signal when a receptor coupled to the particle interacts with the analyte. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized.
    Type: Grant
    Filed: January 31, 2001
    Date of Patent: November 18, 2003
    Assignee: Board of Regents, The University of Texas Systems
    Inventors: John T. McDevitt, Eric V. Anslyn, Jason B. Shear, Dean P. Neikirk
  • Patent number: 6649418
    Abstract: The present invention provides internally calibrated competitive assays for use on a solid support. Additionally, the invention provides a method of using such assays.
    Type: Grant
    Filed: July 30, 2001
    Date of Patent: November 18, 2003
    Assignee: Silver Lake Research Corporation
    Inventor: Mark S. Geisberg
  • Patent number: 6638728
    Abstract: New protein coated surfaces, which have a high capacity for capturing target molecules, thus yielding assays with enhanced sensitivity, are disclosed. Surfaces prepared according to the present invention contain a coating consisting essentially of streptavidin, avidin or “NeutrAvidin” in polymeric form, wherein polymerization has been controlled to an extent such that the polymer is predominantly dimers, trimers and tetramers of the native molecule.
    Type: Grant
    Filed: June 15, 2001
    Date of Patent: October 28, 2003
    Assignee: Pierce Biotechnology, Inc.
    Inventors: Surbhi Desai, Mark Rickerd, Ineabel Horneij
  • Patent number: 6635434
    Abstract: A hapten-polymer carrier complex was found to be useful for immunoassay purposes, specifically ELISAs, for the detection of pesticides and their degradation products in hydrosoil and ground water. The degradation products of Casoron G® (also known as dichlorobenzonitrile and dichlorbenil) and Prefix® (also known as chlorthiamid and dichlorobenzthiamide) are analytes detected with high specificity and sensitivity, particularly the degradation product BAM (2,6-dichlorobenzamide). The polymer carrier complex is bound to the hapten via a linker unit, strategically positioned meta to the amide or amide derivative of BAM.
    Type: Grant
    Filed: July 21, 2000
    Date of Patent: October 21, 2003
    Assignee: Exiqon A/S
    Inventors: Mogens Havsteen Jakobsen, Leif Bruun, Brian Pedersen
  • Patent number: 6632655
    Abstract: Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members.
    Type: Grant
    Filed: February 22, 2000
    Date of Patent: October 14, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: Tammy Burd Mehta, Anne R. Kopf-Sill, J. Wallace Parce, Andrea W. Chow, Luc J. Bousse, Michael R. Knapp, Theo T. Nikiforov, Steve Gallagher
  • Patent number: 6632526
    Abstract: An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.
    Type: Grant
    Filed: October 14, 1998
    Date of Patent: October 14, 2003
    Assignee: Luminex Corporation
    Inventors: Don J. Chandler, Van S. Chandler, Beth A. Lambert, Janet J. Reber, Stacie L. Phipps
  • Patent number: 6632929
    Abstract: A covalent conjugate of a 4′-hydroxyazobenzene-2-carboxylic acid derivative (HABA) and an avidin-type molecule, of the formula: wherein A is (CH2)n or —CH═CH—, wherein n is an integer from 0-10; B is (CH2)n wherein n is an integer from 2 to 10; m is zero or 1; and Av is the residue of an avidin-type molecule selected from the group comprising native egg-white avidin, recombinant avidin, deglycosylated avidins, bacterial streptavidin, recombinant streptavidin, truncated streptavidin and other derivatives of said avidin-type molecules. These HABAylated avidins are red colored in the quinone configuration and can be used in many applications in the avidin-biotin technology.
    Type: Grant
    Filed: August 7, 2001
    Date of Patent: October 14, 2003
    Assignee: Yeda Research and Development Co. LTD
    Inventors: Meir Wilchek, Edward A Bayer, Heike Hofstetter, Margherita Morpurgo
  • Patent number: 6630358
    Abstract: Protein arrays for the parallel, in vitro screening of biomolecular activity are provided. Methods of using the protein arrays are also disclosed. On the arrays, a plurality of different proteins, such as different members of a single protein family, are immobilized on one or more organic thinfilms on the substrate surface. The protein arrays are particularly useful in drug development, proteomics, and clinical diagnostics.
    Type: Grant
    Filed: May 12, 2000
    Date of Patent: October 7, 2003
    Assignee: Zyomyx, Incorporated
    Inventors: Peter Wagner, Dana Ault-Riche, Steffen Nock, Christian Itin
  • Publication number: 20030181606
    Abstract: A process of producing particles for diagnostic reagent, including a step of polymerizing a polymerizable monomer in the presence of a matrix particle having a magnetic substance layer containing at least one of Fe2O3 and Fe3O4 on the surface thereof and having a mean particle size of 0.4-200 &mgr;m to form a polymer layer on the magnetic substance layer.
    Type: Application
    Filed: March 21, 2003
    Publication date: September 25, 2003
    Applicant: JSR CORPORATION
    Inventors: Ichiro Ozaki, Satoshi Katayose, Mikio Hikata, Kejun Fan, Mitsuhiro Murata
  • Patent number: 6620628
    Abstract: An assay element for analyzing a charged analyte employs an immobilized receptor and a material having a net charge which is the same as that of the analyte. In a preferred embodiment, the analyte is an aminoglycoside and the material is a polymer having a net positive charge.
    Type: Grant
    Filed: March 2, 2001
    Date of Patent: September 16, 2003
    Assignee: Ortho-Clinical Diagnostics, Inc.
    Inventors: Richard Sutton, Susan Danielson, Jerome Swartz, Linda Mauck
  • Patent number: 6617142
    Abstract: Methods are provided for forming a coating of an immobilized biomolecule on a surface of a medical device to impart improved biocompatibility for contacting tissue and bodily fluids. A biomolecule such as a glycoprotein having an unsubstituted amide moiety is combined with an amine forming agent to form an amine-functional biomolecule. The amine-functional biomolecule is combined with a medical device surface having a chemical moiety such as aldehyde, epoxide, isocyanate, 1,2-dicarbonyl, phosphate, sulphate or carboxylate to form a chemical bond immobilizing the biomolecule on the surface. The chemical bond may be combined with a reducing agent or a stabilizing agent. The aldehyde moiety may be formed by combining a periodate with a 2-aminoalcohol moiety or a 1,2-dihydroxy moiety. Alternatively, an amine-functional medical device surface is combined with a biomolecule having a chemical moiety that reacts with an amine moiety.
    Type: Grant
    Filed: February 24, 1999
    Date of Patent: September 9, 2003
    Assignee: Medtronic, Inc.
    Inventors: James R. Keogh, Paul V. Trescony
  • Patent number: 6616946
    Abstract: Polymeric hollow particles for delivery of an agent are provided that change permeability in response to a change in an external stimulus such as pH, temperature, light, ionic strength, electric field, magnetic field and/or solvent composition. The particles can have a shell formed of an amphiphilic triblock ABA or BAB copolymer, where A is a hydrophilic block and B is a hydrophobic block. Low permeability particles with a reversibly permeable shell expand and increase permeability in response to a stimulus so that an active agent such as a therapeutic, prophylactic or diagnostic agent can be introduced. Removing the stimulus allows the particles to return to a low permeability state to form particles loaded with the active agent. Surfaces of the particles can be modified with specific ligands that allow the particles to be directed to a specific target via molecular recognition.
    Type: Grant
    Filed: November 15, 2000
    Date of Patent: September 9, 2003
    Assignee: BioCure, Inc.
    Inventors: Wolfgang Meier, Marc Sauer
  • Patent number: 6613512
    Abstract: Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.
    Type: Grant
    Filed: December 5, 2000
    Date of Patent: September 2, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: Anne R. Kopf-Sill, Andrea W. Chow, Claudia B. Jaffe, Steven A. Sunberg, John Wallace Parce
  • Publication number: 20030157732
    Abstract: A biosensor based on complexes between biomolecule receptors and colloidal Au nanoparticles, and more specifically, colloid layers of receptor/Au complexes that can be used to detect biomolecule analytes through measuring of binding-induced changes in electrical resistance or surface plasmon resonance. Also disclosed is a method for detecting and analysing carrier-borne chemical compounds with Raman spectroscopy using an improved SERS substrate. Further disclosed is an improved method for detecting compounds in solvents using capillary electrophoresis in conjunction with Raman spectroscopy.
    Type: Application
    Filed: March 16, 2001
    Publication date: August 21, 2003
    Inventors: Bonnie E. Baker, Michael J. Natan
  • Publication number: 20030139483
    Abstract: A method of making a molecularly imprinted porous structure makes use of a surfactant analog of the molecule to be imprinted that has the imprint molecule portion serving as the surfactant headgroup. The surfactant analog is allowed to self-assemble in a mixture to create at least one supramolecular structure having exposed imprint groups. The imprinted porous structure is formed by adding reactive monomers to the mixture and allowing the monomers to polymerize, with the supramolecular structure serving as a template. The resulting solid structure has a shape that is complementary to the shape of the supramolecular structure and has cavities that are the mirror image of the imprint group. Similarly, molecularly imprinted particles may be made by using the surfactant to create a water-in-oil microemulsion wherein the imprint groups are exposed to the water phase.
    Type: Application
    Filed: January 8, 2003
    Publication date: July 24, 2003
    Inventors: Michael A. Markowitz, Paul E. Schoen, Bruce P. Gaber, Banahalli R. Ratna, Paul R. Kust, David C. Turner, Douglas S. Clark, Jonathan S. Dordick
  • Publication number: 20030134333
    Abstract: Compositions and methods are disclosed for detecting multiple target analytes in a sample using microparticles having molecular tags attached by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such microparticles under conditions that permit stable complexes to form between binding moieties on the surfaces of the microparticles and the analytes. In one aspect of the invention, the a second binding composition is added so that complexes form among the microparticle-bound binding moieties, the analytes, and second binding moieties of the second binding composition. Such second binding moieties have cleavage-inducing moieties attached that upon activation cause the cleavage of the cleavable linkages and the release of molecular tags. Released molecular tags are separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.
    Type: Application
    Filed: November 8, 2002
    Publication date: July 17, 2003
    Inventors: Peter Dehlinger, Sharat Singh, Hrair Kirakossian
  • Patent number: 6589798
    Abstract: The invention relates to methods, kits and systems for determining an analyte in a liquid sample as well as the use thereof for concentration analysis and screening purposes. In one method, a specific binding partner to the analyte is permitted to compete with a conjugate containing the analyte or an analyte analogue for the binding to free analyte. The conjugate also contains a component that specifically binds to a solid support so that reacted and unreacted conjugate are bound thereto when the reaction solution is contacted with the solid support. The amount of analyte is then determined by measuring by a label-free mass-detection technique, such as surface plasmon resonance, the amount of binding partner immobilized on the solid support via the reacted conjugate. This is made possible by the binding partner having a considerably greater mass than the conjugate. Variants of this method are also disclosed.
    Type: Grant
    Filed: June 11, 1999
    Date of Patent: July 8, 2003
    Assignee: Biacore AB
    Inventor: Stefan Löfås
  • Patent number: 6589799
    Abstract: A method for producing a derivatized aldehydic support matrix material includes activating surface hydroxyl groups on the support matrix material and reacting the activated hydroxyl groups with an aldehydic alkoxy silane. The derivatized aldehydic support matrix material produced is useful for immobilizing bio-molecules in biological applications.
    Type: Grant
    Filed: May 2, 2001
    Date of Patent: July 8, 2003
    Assignee: United Chemical Technologies, Inc.
    Inventors: Ann N. Coyne, John H. MacMillan, Michael J. Telepchak
  • Publication number: 20030119058
    Abstract: A method of assaying collagen fragments in body fluids, including bringing a sample of body fluid in contact with at least one immunological binding partner for the collagen fragments, said binding partner being immunoreactive with synthetic peptides, the sequences of which are essentially derived from collagen and containing potential sites for cross-linking. The immunological binding partners are incorporated, either as whole antibodies or as immunologically active fragments thereof, in an assay for quantitative determination of collagen fragments in the sample. In addition to being contacted with the immunological binding partner(s), the sample may be brought into direct contact with the corresponding synthetic peptide. The invention further comprises a test kit and specific means for carrying out the method. The structure of specific peptides is also described.
    Type: Application
    Filed: January 29, 2002
    Publication date: June 26, 2003
    Applicant: Osteometer A/S
    Inventors: Per Qvist, Martin Bonde
  • Patent number: 6582969
    Abstract: Methods for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules that can be immobilized on the surface of the devices of the present invention include proteins, polypeptides, nucleic acids, polysaccharides, phospholipids, and related unnatural plyomers of biological relevance. These devices are useful in high-throughput drug screening and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.
    Type: Grant
    Filed: May 12, 2000
    Date of Patent: June 24, 2003
    Assignee: Zyomyx, Inc.
    Inventors: Peter Wagner, Dana Ault-Riche, Steffen Nock, Christian Itin
  • Patent number: 6583191
    Abstract: A method of making a molecularly imprinted porous structure makes use of a surfactant analog of the molecule to be imprinted that has the imprint molecule portion serving as the surfactant headgroup. The surfactant analog is allowed to self-assemble in a mixture to create at least one supramolecular structure having exposed imprint groups. The imprinted porous structure is formed by adding reactive monomers to the mixture and allowing the monomers to polymerize, with the supramolecular structure serving as a template. The resulting solid structure has a shape that is complementary to the shape of the supramolecular structure and has cavities that are the mirror image of the imprint group. Similarly, molecularly imprinted particles may be made by using the surfactant to create a water-in-oil microemulsion wherein the imprint groups are exposed to the water phase.
    Type: Grant
    Filed: September 19, 2001
    Date of Patent: June 24, 2003
    Inventors: Michael A. Markowitz, Paul E. Schoen, Bruce P. Gaber, Banahalli R. Ratna, Paul R. Kust, David C. Turner, Douglas S. Clark, Jonathan S. Dordick
  • Publication number: 20030113794
    Abstract: A particle-enhanced assay for determining an analyte in a test sample in which an additive for reducing non-specific particle aggregation is added in an amount to substantially reduce non-specific aggregation.
    Type: Application
    Filed: December 7, 2001
    Publication date: June 19, 2003
    Inventors: Kurt L. Brillhart, Julie A. Whiteside
  • Patent number: 6576461
    Abstract: An electrochemical affinity assay system for detection of ligand—ligand receptor binding.
    Type: Grant
    Filed: June 18, 2001
    Date of Patent: June 10, 2003
    Assignee: Therasense, Inc.
    Inventors: Adam Heller, Charles N. Campbell
  • Patent number: 6573110
    Abstract: A combinatorial chemistry system allows for the dual processing of different molecules coated on a library of beads. The system includes beads coated with different molecules on each bead, a bead holder, screening equipment and characterization equipment. Molecules on the beads are both screened and characterized simultaneously.
    Type: Grant
    Filed: December 15, 1999
    Date of Patent: June 3, 2003
    Assignee: The Penn State Research Foundation
    Inventors: Robert J. Hessler, Robert Braun, Nicholas Winograd
  • Patent number: 6558944
    Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.
    Type: Grant
    Filed: July 1, 1999
    Date of Patent: May 6, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: John Wallace Parce, Anne R. Kopf-Sill, Luc J. Bousse
  • Patent number: 6555391
    Abstract: This invention relates methods for conditioning affinity chromatography resins to decrease leaching of the ligand during purification. The methods involve incubating the resin in a buffered solution of a hydroxyalkylamine compound (e.g., ethanolamine) prior to use of the resin for an affinity purification. The treatment removes unstably bound ligand from the resin.
    Type: Grant
    Filed: August 15, 2000
    Date of Patent: April 29, 2003
    Assignee: Baxter International, Inc.
    Inventors: Susan L. Bernhard, Robert Toso, Van Taiariol
  • Patent number: 6551830
    Abstract: Disclosed are novel protein and peptide compositions comprising soluble and bound forms of immunologically-active blood group antigens including mammalian Rh antigens. In preferred embodiments methods for the isolation and purification of serologically-active human Rh antigens such as D, c, C, E, and e are disclosed. Also disclosed are methods for the adsorption of immunologically-active Rh antigens to solid supports. Diagnostic kits, methods, and devices for the detection of Rh antibodies in clinical and non-clinical samples are also disclosed. Devices, compositions and methods for the isolation, purification and quantitation of anti-Rh antibodies from solution are also provided.
    Type: Grant
    Filed: February 20, 2001
    Date of Patent: April 22, 2003
    Assignees: Board of Regents, The University of Texas System, Baylor College of Medicine
    Inventors: L. Scott Rodkey, Marwan A. Yared, Kenneth J. Moise, Jr.
  • Patent number: 6551841
    Abstract: Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.
    Type: Grant
    Filed: January 27, 1999
    Date of Patent: April 22, 2003
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Peter Wilding, Larry J. Kricka, Jay N. Zemel
  • Patent number: 6548310
    Abstract: A particle for a diagnostic agent, which comprises a polymer particle comprising (A) from 20 to 100% by weight of a structural unit derived from at least one of acrylate having an aliphatic hydrocarbon group and methacrylate having an aliphatic hydrocarbon group, (B) from 0 to 10% by weight of a structural unit derived from unsaturated carboxylic acid, and (C) from 0 to 80% by weight of a structural unit derived from a vinyl monomer copolymerizable with the acrylate, methacrylate and unsaturated carboxylic acid, and a turbidmetric immunoassay using the same.
    Type: Grant
    Filed: February 1, 2000
    Date of Patent: April 15, 2003
    Assignee: JSR Corporation
    Inventors: Mitsuhiro Murata, Satoshi Katayose, Kiyoshi Kasai, Mikio Hikata, Toru Masukawa, Masaaki Kitajima
  • Patent number: 6548635
    Abstract: A novel HIV type O immunodeficiency virus is disclosed which has the designation MVP-2901/94 and which has been deposited with the European Collection of Animal Cell Cultures (ECACC) under No. V 950121601. The characteristic antigens which can be obtained from the virus and which can be employed for detecting antibodies against retroviruses which are associated with immunodeficiency diseases are also disclosed, as are the partial DNA and amino acid sequences of the virus.
    Type: Grant
    Filed: July 6, 2000
    Date of Patent: April 15, 2003
    Assignee: Dade Behring Marburg GmbH
    Inventors: Hans-Peter Hauser, Stefan Knapp, Stefan Brust, Lutz G. Gürtler, Josef Eberle, Lazare Kaptue, Léopold Achengui Zekeng
  • Patent number: 6544800
    Abstract: Novel sensor devices composed of a crystalline colloidal array (CCA) polymerized in a hydrogel are disclosed. The hydrogels are characterized as being capable of shrinking and swelling in response to specific stimuli applied thereto. As the hydrogels shrink or swell, the lattice structure of the CCA embedded therein changes, thereby changing the wavelength of light diffracted by the CCA. Thus by monitoring the change in diffracted wavelength, the concentration of a stimulus is determined. The gels can be modified to sense numerous different stimuli. The sensor devices are specific in that they are modified to react with only one species or family of species. These sensors have various applications in areas including, for example, environmental and chemical systems, chemomechanical systems, sensor devices and medical diagnostic tools. Various methods for making and using these devices are also disclosed.
    Type: Grant
    Filed: January 3, 2001
    Date of Patent: April 8, 2003
    Assignee: University of Pittsburgh
    Inventor: Sanford A. Asher
  • Publication number: 20030049870
    Abstract: A selective adsorption material, especially suitable for adsorption of biological macromolecules, is described. The, adsorption material comprises a matrix with immobilised ligands, localised to selectively adsorb a predetermined molecule. A process for preparing a selective adsorption material, especially suitable for adsorption of biological macromolecules, is also described. The process is characterised in that a print molecule having at least two separate binding sites is bonded to the corresponding, at least two immobilisable ligands, the ligands are immobilised, and subsequently the print molecule is removed. The selective adsorption material can be used for purification or analysis, especially of biological macromolecules.
    Type: Application
    Filed: May 5, 1994
    Publication date: March 13, 2003
    Inventors: MAGNUS GLAD, MARIA KEMPE, KLAUS MOSBACH
  • Publication number: 20030049608
    Abstract: Described herein is an ELISA based assay for the detection of HCV infection. This new assay can detect HCV infection earlier than the currently used assays for the screening of blood for HCV infection by using a combination of HCV antigens and anti-core antibodies to capture HCV.
    Type: Application
    Filed: March 28, 2002
    Publication date: March 13, 2003
    Inventors: Chander Bahl, Patrick Niven, Antonio Samson, Denise Madjor
  • Patent number: 6531278
    Abstract: Compositions and methods of use thereof for capture and detection of selected molecules are described. In one embodiment, a first composition includes a ligand component, such as an antibody coupled to a nucleic acid component. An a preferred embodiment, the nucleic acid is labeled with a fluorescent marker to facilitate detection. Another aspect of the invention is the ligand component bound to a solid support via a complementary nucleic acid component and a linker moiety. The method involves binding the target with the first composition in free solution, then binding the target to the solid support by means of both DNA hybridization and antibody-antigen affinity binding. Unbound molecules are washed away, and then the bound targets are detected by fluorescence detection. Vital stains can also be used to detect viable cells.
    Type: Grant
    Filed: January 14, 1999
    Date of Patent: March 11, 2003
    Assignee: Utah State University
    Inventor: Bart Weimer
  • Patent number: 6528321
    Abstract: A chromatographic assay device according to the present invention provides a unidirectional immunoassay for an analyte in a whole blood sample with improved sensitivity and freedom from interference.
    Type: Grant
    Filed: June 26, 2000
    Date of Patent: March 4, 2003
    Assignee: Beckman Coulter, Inc.
    Inventors: Daniel Fitzgerald, Howard M. Chandler, Larry LaPointe
  • Patent number: 6528272
    Abstract: A rapid, simple, and inexpensive sandwich enzyme-linked receptor based immunodot assay detects pathogens or pathogenic products in test samples using receptors for a characteristic component of the pathogen. This assay is widely applicable because it is highly specific, it does not require special equipment, and the results can be obtained within a few hours with the naked eye. Since the lipid-based receptors have a long-shelf life, they can be easily stored and used for a long time.
    Type: Grant
    Filed: February 5, 1998
    Date of Patent: March 4, 2003
    Assignee: The Johns Hopkins University
    Inventor: Subroto Chatterjee
  • Patent number: 6528291
    Abstract: A method is provided for preparing an active slide, including introducing a monomer containing an aldehyde group, or a mixture of a monomer containing an aldehyde group and an acidic functional group provider into a plasma chamber; and depositing the aldehyde group and acidic functional group onto the surface of an organic or inorganic matrix using plasma deposition to form a slide comprising a layer of polymerized actively functional groups thereon. The aldehyde groups and negatively charged groups are deposited on the surface of the active slide, such that the bio-molecules bound thereto possess the properties of an inducible orientation and thus form a mono-layer.
    Type: Grant
    Filed: September 11, 2000
    Date of Patent: March 4, 2003
    Assignee: Industrial Technology Research Institute
    Inventors: Zu-Sho Chow, Jia-Huey Tsao, Wen-Hsun Kuo, Chih-Wei Ho, Bor-Iuan Jan, Chao-Chi Pan, Yao-Sung Chang, Cheng-Tao Wu, Yu-Ching Liu
  • Patent number: 6524790
    Abstract: Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.
    Type: Grant
    Filed: June 8, 1998
    Date of Patent: February 25, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: Anne R. Kopf-Sill, Andrea W. Chow, Claudia B. Cohen, Steven A. Sundberg, John Wallace Parce
  • Patent number: 6514688
    Abstract: Biological materials in a mixture of substances are separated, detected or quantified using magnetic spherically shaped cross-linked polyvinyl alcohol (PVAL) polymer particles ranging in size from 1 to 10 &mgr;m. The particles containing a coupled ligand are used to bind a biological material in a mixture of substances, and the particles containing bound biological material are isolated from the mixture. The particles are prepared by dispersing a magnetic colloid containing a magnetic material such as a ferromagnetic or superparamagnetic substance in an aqueous solution of polyvinyl alcohol containing reactive hydroxyl groups, adding the resultant mixture to an organic phase containing a mixture of at least two emulsifiers, and adding a water-soluble cross-linking agent such as a dialdehyde that reacts with the hydroxyl groups of polyvinyl alcohol to form the polymer particles.
    Type: Grant
    Filed: November 29, 2000
    Date of Patent: February 4, 2003
    Assignee: chemagen Biopolymer-Technologie Aktiengesellschaft
    Inventor: Detlef Muller-Schulte
  • Patent number: 6514734
    Abstract: A polybifunctional reagent is provided having a polymeric backbone, one or more pendent latent reactive (preferably photoreactive) moieties, and two or more pendent bioactive groups. The reagent can be activated to form a bulk material or can be brought into contact with the surface of a previously formed biomaterial and activated to form a coating. The pendent bioactive groups function by promoting the attachment of specific molecules or cells to the bulk material or coated surface. Bioactive groups can include proteins, peptides, carbohydrates, nucleic acids and other molecules that are capable of binding noncovalently to specific and complimentary portions of molecules or cells.
    Type: Grant
    Filed: June 9, 2000
    Date of Patent: February 4, 2003
    Assignee: Surmodics, Inc.
    Inventors: David L. Clapper, Melvin J. Swanson, Sheau-Ping Hu, Richard A. Amos, Terrence P. Everson
  • Patent number: 6514771
    Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.
    Type: Grant
    Filed: October 17, 2000
    Date of Patent: February 4, 2003
    Assignee: Bioarray Solutions
    Inventor: Michael Seul
  • Patent number: 6511812
    Abstract: An improved method for detecting antibodies is disclosed. The method employs a recombinant denatured bacterial enzyme. The invention also relates to a test kit useful for performing an immunoassay which comprises a container containing a denatured recombinant bacterial enzyme.
    Type: Grant
    Filed: November 12, 1996
    Date of Patent: January 28, 2003
    Assignee: Abbott Laboratories
    Inventors: Robert C. Schoen, Yuzo Inoue, Toshinori Takei, Satoshi Jomura, Susan E. Sweeney, Joseph S. Niedbalski
  • Patent number: 6509148
    Abstract: Disclosed is a method for fabricating biosensors, using hydrophilic polyurethane. Bio-active reagents, including enzymes, antibodies, antigens, cells and receptors, are mixed with hydrophilic polyurethane and the mixture is directly coated over a signal transducer to form a sensing film which serves as a signal detector. The method using hydrophilic polyurethane allows the simplification of the fabrication of biosensors without conducting complicated chemical reactions and washing steps, such as crosslinking. The bio-active reagent entrapped within the hydrophilic polyurethane film can retains its high activity for an extended period of time and the intrinsic potentiometric response of the underlying ion-selective polymeric membrane is not affected by the bio-active reagent immobilized polyurethane film coated on its sensing surface. Therefore, the biosensors are superior in specificity, selectivity, and stability.
    Type: Grant
    Filed: February 10, 2000
    Date of Patent: January 21, 2003
    Assignee: i-Sens, Inc.
    Inventors: Geun Sig Cha, Hakhyun Nam, Jae Ho Shin
  • Patent number: 6506342
    Abstract: A combinatorial chemistry bead that includes an electromagnetic spectral emitter that radiates a distinct electromagnetic code for each bead that uniquely identifies each bead, a terminal apparatus for receiving the electromagnetic code from each bead, and a method for performing combinatorial synthesis using a bead that transmits a distinct electromagnetic code. The invention includes a large number of spectrally narrowed light emitting mechanisms for generating distinct optical codes.
    Type: Grant
    Filed: May 15, 2000
    Date of Patent: January 14, 2003
    Inventor: Robert D. Frankel
  • Patent number: 6503722
    Abstract: This invention provides methods, compositions, and kits for detecting the presence of toxigenic strains of C. difficile in a biological sample. One embodiment provides methods for C. difficile detection that involve assaying for both C. difficile glutamate dehydrogenase and C. difficile toxin A or toxin B. In another embodiment, the invention provides a highly sensitive assay for C. difficile toxin A that is useful for determining whether a C. difficile strain is toxigenic. This embodiment involves binding of toxin A to a moiety that reversibly binds to a capture moiety present on a magnetic bead. A magnetic field is applied to the sample to concentrate the toxin A, after which the magnetic beads are dissociated and removed from the solution to obtain a highly concentrated preparation of toxin A, thus making possible a very sensitive assay.
    Type: Grant
    Filed: July 29, 1999
    Date of Patent: January 7, 2003
    Assignee: Biosite Diagnostics
    Inventor: Gunars E. Valkirs
  • Patent number: 6503761
    Abstract: A system and method for removing contaminants from a surface. The system is designed to use particles having means thereon which are capable of selectively binding to a contaminant or contaminants of interest. The particles are applied to the surface whereupon the contaminants bind to the particle. When the particle is removed, the desired contaminants are also removed. Preferably, the present invention utilizes magnetic particles having iron therein. The particles may then be readily removed using magnets. The means for binding the contaminant to the particle preferably comprise a ligand or a charge specifically designed to remove the contaminant of interest. The particles may be included in a carrier to facilitate their application to the surface. The invention is especially useful for the removal of contaminants from skin.
    Type: Grant
    Filed: October 19, 1999
    Date of Patent: January 7, 2003
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventors: David W. Koenig, Brenda M. Nelson, Beth Anne Lange
  • Publication number: 20030003442
    Abstract: A novel immunodeficiency virus is disclosed which has the designation MVP-5180/91 and which has been deposited with the European Collection of Animal Cell Cultures (ECACC) under No. V 920 52 318. The characteristic antigens which can be obtained from it and which can be employed for detecting antibodies against retroviruses which are associated with immunodeficiency diseases are also disclosed, as are the DNA and amino acid sequences of the virus.
    Type: Application
    Filed: June 22, 2001
    Publication date: January 2, 2003
    Applicant: Dade Behring Marburg GmbH
    Inventors: Lutz G. Guertler, Josef Eberle, Albrecht V. Brunn, Stefan Knapp, Hans-Peter Hauser