Carrier Is Water Suspendible Particles Patents (Class 436/534)
  • Patent number: 7879625
    Abstract: Improved surface-enhanced Raman scattering (SERS) substrates comprising chemically-derivatized magnetic microparticles complexed with metal colloidal particles or substrates. The SERS substrates permit collection, detection, measurement, and/or analysis of analytes present at concentrations ranging parts per trillion to parts per billion. Further, compositions, methods, and devices that provide for rapid and/or sensitive detection of chemical compounds of interest present in small concentrations. The subject matter has use in the areas of homeland security and force protection, for example, in the detection of trace samples including, for example, BTEX (benzene, toluene, ethylbenzene, and xylenes), chlorinated solvents, TNT, nerve agents, blister agents, metal ions, anions, antigens, peptides, nucleic acids, spores, fungi, viruses, and bacteria.
    Type: Grant
    Filed: December 3, 2008
    Date of Patent: February 1, 2011
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventor: Pamela A. Boss
  • Patent number: 7867785
    Abstract: An objective of the invention is to provide a carrier particle latex for an assay reagent capable of assaying a biological sample at a wide range of the concentration in an immunoserological test and capable of being stored stably for a prolonged period, as well as an assay reagent employing the same. The invention is a carrier particle latex for an assay reagent comprising a carrier particle comprising a copolymer of a polymerizable monomer having a phenyl group and a polymerizable monomer having a phenyl group and a sulfonate, wherein said carrier particle has a surface sulfonic acid group amount of 0.005 to 0.7 ?mol/m2 and an average particle size of 0.01 to 1.5 ?m.
    Type: Grant
    Filed: January 3, 2008
    Date of Patent: January 11, 2011
    Assignee: Sekisui Medical Co., Ltd.
    Inventor: Satoshi Obana
  • Patent number: 7824873
    Abstract: A blood crossmatching apparatus, kit and methods for testing the compatibility of mammals for blood transfusion. Particulate layers in the apparatus allow nonagglutinated red blood cells to permeate through, while agglutinated red blood cells cannot. The apparatus also has a density solution. The density solution separates white blood cells from red blood cells in the whole blood when centrifuged, without lysing the red blood cells. Thus, the apparatus can be used to test whole blood.
    Type: Grant
    Filed: October 24, 2008
    Date of Patent: November 2, 2010
    Inventor: Anne S. Hale
  • Patent number: 7824927
    Abstract: Analytes using an active assay may be detected by introducing an analyte solution containing a plurality of analytes to a lacquered membrane. The lacquered membrane may be a membrane having at least one surface treated with a layer of polymers. The lacquered membrane may be semi-permeable to nonanalytes. The layer of polymers may include cross-linked polymers. A plurality of probe molecules may be arrayed and immobilized on the lacquered membrane. An external force may be applied to the analyte solution to move the analytes towards the lacquered membrane. Movement may cause some or all of the analytes to bind to the lacquered membrane. In cases where probe molecules are presented, some or all of the analytes may bind to probe molecules. The direction of the external force may be reversed to remove unbound or weakly bound analytes. Bound analytes may be detected using known detection types.
    Type: Grant
    Filed: April 5, 2006
    Date of Patent: November 2, 2010
    Assignee: George Mason Intellectual Properties, Inc.
    Inventors: Victor Morozov, Charles L. Bailey, Melissa R. Evanskey
  • Patent number: 7732160
    Abstract: Compounds of methamphetamine derivatives having a meta-substituted alkyl linker on the benzene ring and a protective group on the nitrogen of the methamphetamine hapten. Such compounds have the structure. wherein R1 is an alkyl linker comprising 2-15 carbon atoms and 0-6 heteroatoms, R2 is a leaving group, and R3 is a protecting group.
    Type: Grant
    Filed: October 1, 2007
    Date of Patent: June 8, 2010
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Raymond A. Hui, Stephen S. Vitone
  • Patent number: 7709273
    Abstract: Methods, compositions and kits are disclosed. The compositions are light emitting and comprise a polymeric matrix having dissolved therein a photoactive compound. The composition has the characteristic that, after activation of the photoactive compound, the rate of decrease in the intensity of light emission at any time during a 20-fold decrease in the intensity is proportional to the intensity of the light emission. In one embodiment the polymeric matrix is comprised of particles of about 20 nm to about 100 ?m in diameter to which is bound a specific binding pair member. The particles generally comprise a polymeric matrix having dissolved therein about 1 to about 20% by weight of a dopant. The compositions may be used in methods for determining an analyte. A combination is provided comprising (1) a medium suspected of containing the analyte, (2) and the aforementioned composition. The photoactive substance is activated and the effect of the activating on the optical properties of the combination is detected.
    Type: Grant
    Filed: November 14, 2005
    Date of Patent: May 4, 2010
    Assignee: Siemens Healthcare Diagnostics Products GmbH
    Inventors: Sharat Singh, John S. Pease, Jacqueline Sadakian, Daniel B. Wagner, Edwin F. Ullman
  • Patent number: 7659125
    Abstract: A latex reagent for analyzing adiponectin, comprising a suspension of latex particles carrying a substance which specifically binds to adiponectin, is disclosed. Further, a method for analyzing adiponectin, comprising (1) obtaining a biological liquid possibly containing adiponectin, and (2) bringing the biological liquid, while maintaining the state in which the biological liquid is obtained, into contact with a suspension of latex particles carrying a substance which specifically binds to adiponectin, and optically analyzing a degree of latex-particles-agglutination, is disclosed. According to the latex reagent and the method for analyzing adiponectin, a predilution or pretreatment of the biological liquid to be analyzed is not necessary. Further, the analysis can be performed rapidly and conveniently, and facilities therefor are not limited.
    Type: Grant
    Filed: March 24, 2004
    Date of Patent: February 9, 2010
    Assignee: Mitsubishi Kagaku Latron, Inc.
    Inventors: Tetsuya Tachikawa, Suguru Akamatsu, Tokio Sawai, Ayako Nishimura
  • Patent number: 7659107
    Abstract: A rapid immunochromatographic assay system is provided for measuring the amount of glycated albumin in a blood sample relative to the total level of albumin in the sample. The assay system is comprised of a disposable cassette that contains the test strips and testing reagents, and a measurement device that automatically reads, calculates and displays the test results over a period of time. The test cassette contains two test strips that are used to measure glycated albumin and total albumin respectively. The strips are contiguous beneath the single sample application well so that the same sample is tested simultaneously by both test strips. Part of the sample will migrate thru the glycated albumin test strip where it will react with the glycated albumin test reagents to yield a glycated albumin result, while part of the sample will migrate thru the total albumin test strip where it will react with the total albumin test reagents to yield a total albumin result.
    Type: Grant
    Filed: September 22, 2004
    Date of Patent: February 9, 2010
    Assignee: Epinex Diagnostics, Inc.
    Inventors: Henry J. Smith, Asad Zaidi
  • Patent number: 7651841
    Abstract: A flow-through assay for detecting the quantity of an analyte residing in a test sample is provided. The flow-through assay contains a porous membrane that is in fluid communication with probe conjugates that contain a specific binding member and a detectable probe. The porous membrane also defines a detection zone and a calibration zone. The calibration zone contains a polyelectrolyte substantially non-diffusively immobilized on the porous membrane. The polyelectrolyte is capable of generating a detectable calibration signal that can be readily compared (visually, quantitatively, and the like) to a detection signal to determine the amount of analyte in the test sample.
    Type: Grant
    Filed: April 25, 2002
    Date of Patent: January 26, 2010
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventors: Xuedong Song, Ning Wei, Curt Sayre
  • Patent number: 7645614
    Abstract: The present invention provides a method of analyzing the specific interaction between a molecule to be analyzed and a molecule that specifically interacts with the former molecule on a solid phase using a molecule-immobilized solid phase support mixture prepared by binding the subject molecule to the solid phase support without specifying the binding position on the molecule side, particularly a method wherein the immobilization is conducted via a spacer introduced to the molecule without specifying the binding position on the molecule side, which method makes it possible to identify and select only a molecule that exhibits a specific interaction with a molecule to be analyzed, without an investigation of structure-activity correlation, which has conventionally been essential, and hence enables an analysis of the interaction between these molecules.
    Type: Grant
    Filed: October 31, 2003
    Date of Patent: January 12, 2010
    Assignee: Reverse Proteomics Research Institute Co., Ltd.
    Inventors: Akito Tanaka, Tomohiro Terada, Akira Yamazaki, Tsuruki Tamura, Hidenori Nakajima
  • Patent number: 7642060
    Abstract: A method and system for the detection and identification of a target pathogen in a sample is disclosed. The method and system involve the use of a capture matrix throughout resuscitation, growth, and detection. The method includes the steps of: placing the sample in a sampling container containing a medium (preferably a growth/resuscitation medium); optionally homogenizing the sample; placing a capture matrix in the sampling container with the sample; incubating the sample in the medium for a pre-determined amount of time at an appropriate temperature; optionally removing the capture matrix from the sampling container; and detecting the presence of the target pathogen in the sample.
    Type: Grant
    Filed: September 24, 2007
    Date of Patent: January 5, 2010
    Assignee: Becton, Dickinson and Company
    Inventors: Mandar S. Nagar, Edward C. McFarland
  • Patent number: 7625765
    Abstract: The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies polymer-microparticle composites including the AC electric field-mediated assembly of patterned, self supporting organic (polymeric) films and organic (polymeric)-microparticle composite films of tailored composition and morphology; the present invention further relates to the incorporation of said assemblies into other structures. The present invention also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, micro-reactors, smart materials. Miniaturized format for generation of multifunctional thin films. Provides a simple set-up to synthesize thin films of tailored composition and morphology.
    Type: Grant
    Filed: October 19, 2005
    Date of Patent: December 1, 2009
    Assignee: Bio Array Solutions Ltd.
    Inventors: Sukanta Banerjee, Kairali Podual, Michael Seul
  • Patent number: 7618784
    Abstract: Improved methods for assaying the activity of phospholipid transfer protein (PLTP) enhance the signal-to-noise ratio by providing the substrate in donor particles with an aqueous core in the presence of suitable osmotic pressure.
    Type: Grant
    Filed: October 23, 2002
    Date of Patent: November 17, 2009
    Assignee: Roar Holding LLC
    Inventors: Robert W. Brocia, Xian-Cheng Jiang
  • Patent number: 7615376
    Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.
    Type: Grant
    Filed: August 27, 2008
    Date of Patent: November 10, 2009
    Assignee: Bayer Schering Pharma Aktiengesellschaft
    Inventors: Thomas Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt
  • Patent number: 7615369
    Abstract: A protein patterning electrode device consisting of capacitor microelectrode arrays coated with a protein non-adherent layer is provided. Operation of the electrode is based on a phenomenon called “electrowetting,” where surface wettability can dynamically be controlled by varying the voltage across the device electrodes. When an electric field is applied across the electrode layers, the surface accumulates charge and becomes hydrophilic, binding the proteins to the surface via ionic bonding. Electrically controlling the amount of the surface charge permits controlled protein surface affinity. The device provides a means for reconfigurable protein patterning.
    Type: Grant
    Filed: September 22, 2005
    Date of Patent: November 10, 2009
    Assignee: The Regents of the University of Michigan
    Inventors: Chih-Tin Lin, Amaya Frost, Edgar Meyhöfer, Katsuo Kurabayashi, Chao Yung Fan
  • Patent number: 7611855
    Abstract: Using two types of antibodies, i.e., a first antibody having a higher affinity for a target substance than for a competitive substance and a second antibody having a higher affinity for the competitive substance than for the target substance, a specimen is treated with these two antibodies. Then, the competitive substance in the specimen first binds to the second antibody and thus the ratio of the target substance to the competitive substance in the specimen is enlarged. As a result, the target substance becomes liable to bind to the first antibody and, in its turn, the reactivity of the target substance is elevated compared with the case of using the first antibody alone. Thus, the target substance in the specimen can be accurately assayed while avoiding the effects of the competitive substance contained in the specimen.
    Type: Grant
    Filed: December 24, 2003
    Date of Patent: November 3, 2009
    Assignee: Nitto Boseki Co., Ltd.
    Inventors: Tatsuya Ohashi, Toshihide Miura, Yoshihiko Igarashi, Fumio Nomura, Takeshi Tomonaga, Katsuhiro Katayama
  • Patent number: 7582488
    Abstract: Disclosed are gel-coated beads (including Hydrogel™-coated beads), which are capable of adsorbing, or absorbing, proteins and other biomolecules onto or into the gel coating. The gel-coated beads with absorbed or adsorbed biomolecules are suitable for use in an assays, purification or other purposes. The beads have a core made from any of a number of materials, including latex, coated with the gel shell. The biomolecules can be retained within the gel, following adsorption, by covalent attachment, or, by selection of conditions of ambient pH and/or ionic strength such that they are retained without further reaction. Therefore, adsorbed proteins would retain the ability to bind to their respective ligands.
    Type: Grant
    Filed: October 26, 2004
    Date of Patent: September 1, 2009
    Assignee: Bio Array Solutions Ltd.
    Inventors: Sukanta Banerjee, Enqing Tan
  • Patent number: 7572642
    Abstract: The invention provides a particle comprising a surface, wherein a portion of the surface is capable of emitting electromagnetic radiation and another portion of the surface is capable of emitting a differential electromagnetic radiation (either different intensity, different frequency or no radiation), and wherein the arrangement of said portions of the surface defines a spatially distributed code for identifying the particle. The invention also provides method of manufacturing a particle having an identifying code comprising providing a particle with a functionalized surface which comprises functional binding moieties and selectively removing a plurality of the functional binding moieties from the surface to create a pattern of functionalized and differentially-functionalized zones on the surface. Various liquid-based assay methods employing the particle of the invention and a kit comprising the particle of the invention are also disclosed.
    Type: Grant
    Filed: April 17, 2002
    Date of Patent: August 11, 2009
    Assignee: Ambrigen, LLC
    Inventors: Nigel G. Skinner, Paul Fenton, George A. Adaniya
  • Patent number: 7569398
    Abstract: The present invention relates to a method of transporting an analyte present in a sample, to a method of concentrating an analyte present in a sample, and to a device for implementing these methods. In the method of transporting an analyte present in a sample of the present invention, a solution A in which the analyte is attached to magnetic particles is prepared from the sample; the solution A is introduced into a first container connected via a bottleneck to a second container; and the analyte attached to the magnetic particles is moved, by means of a magnetic system, from the first container to the second container via the bottleneck, the second container being filled with all or part of the solution A and/or with another solution.
    Type: Grant
    Filed: November 27, 2001
    Date of Patent: August 4, 2009
    Assignee: Commissariat a l'Energie Atomique
    Inventors: Pierre Puget, Patrick Pouteau, Frédéric Ginot, Patrice Caillat
  • Patent number: 7537907
    Abstract: A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are added simultaneously or sequentially to a suspension of cells and bind the cells they are adapted to bind. Cells may bind to one or more microspheres. The suspension is then filtered to trap bead-cell complexes. The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A kit and apparatus for performing the method are also provided.
    Type: Grant
    Filed: February 6, 2008
    Date of Patent: May 26, 2009
    Inventor: Dan A. Pankowsky
  • Patent number: 7504265
    Abstract: A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.
    Type: Grant
    Filed: June 5, 2006
    Date of Patent: March 17, 2009
    Assignee: Lawrence Livermore National Security, LLC
    Inventors: David S. Clague, Elizabeth K. Wheeler, Abraham P. Lee
  • Patent number: 7494824
    Abstract: An interactive system is provided with at least one active surface of plastic from monomers containing at least one structural element derived from a carbon dioxide (A), and at least one substance associated to a linker with at least one structural element (B) capable of establishing a hydrogen bond, and involving an interaction between the structural elements (A) and (B). The interactive system is suitable for presenting and eliminating substances in liquids.
    Type: Grant
    Filed: June 29, 2005
    Date of Patent: February 24, 2009
    Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V., Berlin
    Inventors: Elke Bucha, Götz Nowak
  • Patent number: 7494782
    Abstract: A method for determining the concentration of factor VIIa-antithrombin complexes is disclosed which has application to estimating the level of intravascular exposure of tissue factor, assessing patient risk for hypercoagulation or other coagulopathies, and monitoring patients for factor VIIa-antithrombin complexes over time which can reveal changes in risk for hypercoagulation or other coagulopathies and/or effectiveness of anticoagulant therapy. Antibodies suitable for use in an in vitro assay for determining the concentration of factor VIIa-antithrombin complexes and methods for making the same are also disclosed.
    Type: Grant
    Filed: July 2, 2002
    Date of Patent: February 24, 2009
    Inventor: James H. Morrissey
  • Patent number: 7485472
    Abstract: The invention relates to measuring of the adhesion of platelets ex vivo. In this method, monodisperse polymer particles are added to blood, and the number of platelets in the blood are measured before and after shaking of the sample. The level of adhesion of the platelets is determined by comparing the number of platelets prior to shaking with the number of platelets after shaking. Methods of establishing an adhesion index and diagnosis of a patient are also provided.
    Type: Grant
    Filed: August 9, 2001
    Date of Patent: February 3, 2009
    Assignee: JenAffin GmbH
    Inventors: Gotz Nowak, Elke Bucha
  • Publication number: 20090029483
    Abstract: A reagent for latex aggregation reaction contains water-soluble polymer having a repeating unit presented by the following formula (1): wherein R1 and R2 each independently represents a hydrogen atom or a substituted or unsubstituted alkyl group having 1 to 8 carbon atoms.
    Type: Application
    Filed: July 21, 2008
    Publication date: January 29, 2009
    Applicant: JSR CORPORATION
    Inventors: Kejun FAN, Kouji Tamori
  • Patent number: 7449329
    Abstract: A blood crossmatching apparatus, kit and methods for testing the compatibility of mammals for blood transfusion. Particulate layers in the apparatus allow nonagglutinated red blood cells to permeate through, while agglutinated red blood cells cannot. The apparatus also has a density solution layered above particulate layers. The density solution separates white blood cells from red blood cells in the whole blood when centrifuged, without lysing the red blood cells. Thus, the apparatus can be used to test whole blood.
    Type: Grant
    Filed: March 31, 2006
    Date of Patent: November 11, 2008
    Inventor: Anne S. Hale
  • Publication number: 20080268552
    Abstract: The present invention relates to processes for preparing carboxylated latex particles based on the copolymerization of a monoalkenylaromatic monomer (A) and an aliphatic unsaturated carboxylic acid (B), where the copolymerization takes place at a pH in the range from pH >1.5 to pH <4.5. The pH is typically monitored during the copolymerization process and regulated by suitable compensatory titration. The invention likewise relates to the carboxylated latex particles obtainable by one of the processes according to the invention, and to the use of these particles in an immunological test method.
    Type: Application
    Filed: December 17, 2007
    Publication date: October 30, 2008
    Inventors: Albert Geiger, Gernot Frackmann, Hans-Ulrich Moritz, Peter Sluka
  • Patent number: 7442553
    Abstract: A method for quantitatively determining the amount of maleimidyl groups on a polymer fine particle containing at least maleimidyl groups, by allowing a fluorescent material having an SH group to react with the maleimidyl groups and determining the amount of maleimidyl groups by means of measuring the fluorescence of the fluorescent material.
    Type: Grant
    Filed: February 9, 2005
    Date of Patent: October 28, 2008
    Assignee: Fuji Xerox Co., Ltd.
    Inventors: Takako Kobayashi, Hiroshi Yamamoto, Chisato Urano, Yoshihiro Inaba
  • Patent number: 7390677
    Abstract: An immunoassay comprises the steps of: (a) mixing a whole blood sample with sensitized insoluble carrier particles smaller than erythrocytes to cause an immune agglutination reaction; (b) introducing the resulting immune agglutination reaction mixture including agglutinated particles and unagglutinated particles to a flow cell, irradiating the particles passing through the flow cell with laser light, and detecting scattered lights generated thereby; (c) setting a threshold value for distinguishing unagglutinated particles from agglutinated particles and a threshold value for distinguishing the agglutinated particles from blood cells with regard to intensity of the scattered light; and (d) distinguishing and counting the unagglutinated particles, the agglutinated particles and the blood cells from the scattered lights detected in the step (b), in reference to the threshold values set in the step (c).
    Type: Grant
    Filed: June 11, 2001
    Date of Patent: June 24, 2008
    Assignee: Sysmex Corporation
    Inventors: Kazuhiro Nakashima, Tsuneyoshi Torii, Hiroshi Tsuchiya, Shinya Uchida, Aya Konishi, Kunio Tanaka
  • Publication number: 20080102537
    Abstract: A method of detecting analytes in a microfluidic sample includes introducing at least one type of analyte, at least one type of carrier particle, and a fluid into a mixing chamber of a system. The at least one type of analyte binds to a site on the at least one type of carrier particle to form a microfluidic suspension including at least one analyte-bound particle suspended in the fluid. The at least one analyte-bound particle is separated from the fluid by exposing the suspension to physical forces, magnetic forces, or combinations thereof. A spectrophotometric property of a solution is altered using the at least one analyte-bound particle. The altered spectrophotometric property is measured with an optical detection system.
    Type: Application
    Filed: October 31, 2006
    Publication date: May 1, 2008
    Inventors: Philip H. Harding, Christopher C. Beatty, Kenneth J. Ward
  • Patent number: 7364906
    Abstract: A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody-coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are contacted simultaneously or sequentially with a suspension of cells and bind the cells they are adapted to bind to form bead-cell complexes. Cells may bind to one or more microspheres. The bead-cell complexes are then separated from the suspension The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A method of quantitating a specific cell type is provided. A kit and apparatus for performing the method are also provided.
    Type: Grant
    Filed: December 18, 2003
    Date of Patent: April 29, 2008
    Inventor: Dan A. Pankowsky
  • Patent number: 7351589
    Abstract: A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are added simultaneously or sequentially to a suspension of cells and bind the cells they are adapted to bind. Cells may bind to one or more microspheres. The suspension is then filtered to trap bead-cell complexes. The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A kit and apparatus for performing the method are also provided.
    Type: Grant
    Filed: November 12, 2004
    Date of Patent: April 1, 2008
    Inventor: Dan A. Pankowsky
  • Patent number: 7338813
    Abstract: An objective of the invention is to provide a carrier particle latex for an assay reagent capable of assaying a biological sample at a wide range of the concentration in an immunoserological test and capable of being stored stably for a prolonged period, as well as an assay reagent employing the same. The invention is a carrier particle latex for an assay reagent comprising a carrier particle comprising a copolymer of a polymerizable monomer having a phenyl group and a polymerizable monomer having a phenyl group and a sulfonate, wherein said carrier particle has a surface sulfonic acid group amount of 0.005 to 0.7 ?mol/m2 and an average particle size of 0.01 to 1.5 ?m.
    Type: Grant
    Filed: July 2, 2002
    Date of Patent: March 4, 2008
    Assignee: Sekisui Chemical Co., Ltd.
    Inventor: Satoshi Obana
  • Patent number: 7309723
    Abstract: The invention provides nanoparticles comprising one or more redox-active species, methods of making such nanoparticles, and methods for using such nanoparticles, for example, as diagnostic agents for the detection of various analytes.
    Type: Grant
    Filed: February 18, 2005
    Date of Patent: December 18, 2007
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Marc D. Porter, Jennifer H. Granger
  • Patent number: 7297554
    Abstract: A competitive immunoassay, dye and method for rapidly detecting the presence of one or more target ligands within a fluid sample suspected of containing such ligand or ligands. The immunoassay comprises a protein-binding membrane, a first absorbent pad, a second absorbent pad, and a third absorbent pad. The protein-binding membrane has at least two regions of antibodies bound thereto for detecting dissimilar ligands. The second absorbent pad has formed therein a colloidal gold tracer having one, and preferably two or more ligand analog protein complexes adhering thereto. To utilize the system, the immunoassay strip is placed within a fluid sample. To the extent the target ligand is absent, a visual indicator will be provided signaling such absence. To the extent the target ligand is present at or above a threshold level, no such visual signal will be produced.
    Type: Grant
    Filed: October 28, 2003
    Date of Patent: November 20, 2007
    Assignee: Microdiagnostics, Inc.
    Inventors: Roy Chung, June Shozi
  • Patent number: 7262063
    Abstract: The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies polymer-microparticle composites including the AC electric field-mediated assembly of patterned, self supporting organic (polymeric) films and organic (polymeric)—microparticle composite films of tailored composition and morphology; the present invention further relates to the incorporation of said assemblies into other structures. The present invention. also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, micro-reactors, smart materials. Miniaturized format for generation of multifunctional thin films.
    Type: Grant
    Filed: December 26, 2001
    Date of Patent: August 28, 2007
    Assignee: Bio Array Solutions, Ltd.
    Inventors: Sukanta Banerjee, Kairali Podual, Michael Seul
  • Patent number: 7229835
    Abstract: A compound linked to a solid support (R) through a divalent linker moiety (X) and which is represented by the following formula: is disclosed. In particular, the 1-hydroxybenzotriazole-6-carboxylic acid is directly linked to the support under mild conditions (i.e., in aqueous or organic solvents at neutral pH and at room temperature). The polymer bound 1-hydroxybenzotriazole-6-carboxylic acid can be used for the derivatization of amines as well as for single step amino group modification of proteins, peptides, and amines via acylation or sulfonylation reactions. A flow through device and method for the single step amino group modifications of proteins, peptides, and amines is disclosed. Also disclosed is a flow through device for the detection of amines in a sample. Additionally, a device and method for the detection of amines in a sample using 1-hydroxybenzotriazole-6-carboxylic acid is disclosed. In a preferred embodiment, the device is used to detect the presence of amines in a spoiled meat product.
    Type: Grant
    Filed: October 25, 2001
    Date of Patent: June 12, 2007
    Assignee: The University of Maryland, Baltimore County
    Inventor: Aristotle G. Kalivretenos
  • Patent number: 7205162
    Abstract: Artificial antibodies or antibody mimics are described. They consist of polymers that carry specific binding sites mimicking the properties of antibodies. There is also described a method for producing artificial antibodies, in which polymerisable monomers carrying functional groups and crosslinking monomers are polymerised in the presence of a print molecule and subsequently the print molecule is removed leaving specific binding sites complementary to the print molecules. There are also described methods for determination and isolation of organic molecules using the artificial antibodies as well as therapeutic and diagnostic methods using these antibodies.
    Type: Grant
    Filed: May 6, 1999
    Date of Patent: April 17, 2007
    Inventors: Klaus Mosbach, Georg Vlatakis, Lars I. Andersson, Ralf Müller
  • Patent number: 7202092
    Abstract: Derivatives and conjugates of indinavir for generation of antibodies and labeled conjugates for use for detection of indinavir in biological samples. The derivatives are synthesized out of the indane ring hydroxyl group or the pyridine ring nitrogen of indinavir. Also disclosed is synthesis of a major metabolite of indinavir (M6) in a single step from indinavir using palladium catalyst and hydrogen gas. Indinavir M6 has been extended to synthesize various analogs of indinavir with suitable functional groups. These derivatives are useful in the development of indinavir immunogens, antibodies, and labeled conjugates in the development of indinavir immunoassays.
    Type: Grant
    Filed: November 12, 2004
    Date of Patent: April 10, 2007
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Mitali Ghoshal, Gerald Sigler, Aniruddha P. Patwardhan
  • Patent number: 7192786
    Abstract: This invention relates to a biospecific assay method, in which microparticles coated with the bioaffinity reactant A binding the analyte to be assayed; the sample to be analyzed, and the labelled bioaffinity reactant B are mixed. After the binding reaction the signal strength from the labelled bioaffinity reactant B bound to the microparticles is quantitated for the determination of the concentration of the analyte in the sample. According to the invention, such an amount of sample and microparticles is used in the assay that after binding of the analyte of the sample to the said amount of microparticles, each individual microparticle will emit such a signal strength as to allow the measurement of the analyte concentration over the whole range of typical analyte concentrations, and the signal strength from each microparticle is measured separately.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: March 20, 2007
    Assignee: Wallac Oy
    Inventors: Timo Nils-Erik Lövgren, Antti Juhana Iitiä, Kim Sverker Immanuel Pettersson
  • Patent number: 7192724
    Abstract: A method for aiding in differentiating irritable bowel syndrome from inflammatory bowel disease by determining the level of total endogenous human lactoferrin in clinical specimens, such as feces, mucus and bile, wherein an elevated level of lactoferrin substantially precludes diagnoses of IBS and other noninflammatory etiologies, and a kit usable in such method are provided. Further provided is a method for quantitating the level of total endogenous human lactoferrin in clinical specimens, such as feces, mucus and bile, to monitor gastrointestinal inflammation in persons having inflammatory bowel disease.
    Type: Grant
    Filed: November 14, 2001
    Date of Patent: March 20, 2007
    Assignee: Techlab, Inc.
    Inventors: James Hunter Boone, David Maxwell Lyerly, Tracy Dale Wilkins, Richard Littleton Guerrant
  • Patent number: 7179660
    Abstract: A polysaccharide coated carrier having a coating of at least two successive layers of polysaccharide. The first polysaccharide layer spontaneously associates with a second polysaccharide layer and, optionally, the carrier. Each successive layer of polysaccharide spontaneously associates with a preceding layer. Spontaneous association occurs due to the presence of oppositely charged functional groups on each layer of polysaccharide or due to a spontaneous reaction between the functional groups the layers. The carrier may be any surface such as a tube, microtitration plate, bead, particle or the like and is suitable for use in diagnostic or therapeutic methods.
    Type: Grant
    Filed: March 6, 2000
    Date of Patent: February 20, 2007
    Assignee: Dade Behring Marburg GmbH
    Inventors: Hrair Kirakossian, John S. Pease, Carsten Schelp, Marcel R. Pirio, Uwe Stöhr, Andreas Wiegand
  • Patent number: 7176036
    Abstract: Methods and devices for electrochemical detection of a specific binding pair member utilizing a microsphere with an incorporated electroactive marker, wherein a member of the specific binding pair to be detected is bound, directly or through one or more intermediates, to the microsphere. Multiple specific binding pair members may be detected by use of electrochemically distinguishable electroactive markers. Microspheres with incorporated electroactive markers may include one or more functional groups for binding members of specific binding pairs, and are preferably insoluble in aqueous solvents but soluble in selected organic solvents.
    Type: Grant
    Filed: September 18, 2003
    Date of Patent: February 13, 2007
    Assignee: Arrowhead Center, Inc.
    Inventors: Joseph Wang, Ronen Polsky, Kathryn L. Turner
  • Patent number: 7166477
    Abstract: A method of preparing a sample of muscle tissue and of assaying the prepared sample to determine the presence of prions in the sample is disclosed. The muscle tissue is homogenized and mixed with a complexing agent which forms a complex with a higher specific gravity than PrPSc, the complexing agent or other components of the homogenate. Gravity is then used (e.g. ultra centrifugation) to concentrate the complex and the concentrate is assayed to detect prions. The muscle tissue is preferably extracted from a muscle or group of muscles such as hind limb muscle which have a higher or more preferably the highest concentration of prions as compared to other muscle in the mammal.
    Type: Grant
    Filed: August 2, 2002
    Date of Patent: January 23, 2007
    Assignee: The Regents of the University of California
    Inventors: Stanley B. Prusiner, Patrick Bosque
  • Patent number: 7148024
    Abstract: Novel conjugates of busulfan and novel busulfan immunogens derived from ?-substituted derivatives of busulfan and antibodies generated by these busulfan linked immunogens are useful in immunoassays for the quantification and monitoring of busulfan in biological fluids.
    Type: Grant
    Filed: September 13, 2005
    Date of Patent: December 12, 2006
    Assignee: Saladax Biomedical Inc.
    Inventors: Salvatore J. Salamone, Jodi Blake Courtney, Shu He
  • Patent number: 7144742
    Abstract: This invention provides solid phase specific binding lateral flow assay methods, devices and kits for quantitating high and low molecular weight analytes. The methods and devices of the invention employ labelled reagents which are either analyte analogs or complementary specific binding pair members for the analyte and a novel arrangement of capture zones comprising immobilized specific binding substances for either the analyte or the labelled reagent to effect bound from unbound labelled reagent as a function of analyte concentration. The capture zones are disposed on a non-bibulous matrix defining a flow path from a sample receiving zone to the capture zone. The devices of this invention also include multilane flow paths and multiple capture zones to quantitate analyte.
    Type: Grant
    Filed: December 17, 2004
    Date of Patent: December 5, 2006
    Assignee: Quidel Corporation
    Inventors: Hans Boehringer, Gerald Rowley, Allan D. Pronovost
  • Patent number: 7144119
    Abstract: An apparatus providing programmable illumination pattern generation for the manipulation of colloidal particulates and biomolecules in suspension between electrodes, is disclosed. The apparatus implements LEAPS (Light-controlled electrokinetic assembly of particles near surfaces), which relies on: AC electric field-induced assembly of particles the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The apparatus generates patterns of illumination and projects then onto planar surfaces, i.e., a LEAPS electrode.
    Type: Grant
    Filed: January 24, 2001
    Date of Patent: December 5, 2006
    Assignee: BioArray Solutions Ltd.
    Inventors: Michael Seul, Chiu Wo Chau
  • Patent number: 7141431
    Abstract: This invention provides a novel fluorescent particle including a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer particles, and various applications and methods of using such particles are claimed.
    Type: Grant
    Filed: June 30, 2005
    Date of Patent: November 28, 2006
    Assignee: Luminex Corporation
    Inventors: Mark B. Chandler, Don J. Chandler, Jason Bedre
  • Patent number: 7109042
    Abstract: An analytical test device useful for example in pregnancy testing, comprises a hollow casing (500) constructed of moisture-impervious solid material, such as plastics materials, containing a dry porous carrier (510) which communicates indirectly with the exterior of the casing via a bibulous sample receiving member (506) which protrudes from the casing such that a liquid test sample can be applied to the receiving member and permeate therefrom to the porous carrier, the carrier containing in a first zone a labelled specific binding reagent is freely mobile within the porous carrier when in the moist state, and in a second zone spatially distinct from the first zone unlabelled specific binding reagent for the same analyte which unlabelled reagent is permanently immobilised on the carrier material and is therefore not mobile in the moist state, the two zones being arranged such that liquid sample applied to the porous carrier can permeate via the first zone into the second zone, and the device incorporating mea
    Type: Grant
    Filed: February 12, 2001
    Date of Patent: September 19, 2006
    Assignee: Inverness Medical Switzerland GmbH
    Inventors: Keith May, Michael Evans Prior, Ian Richards
  • Patent number: 7063952
    Abstract: In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11.
    Type: Grant
    Filed: September 28, 2001
    Date of Patent: June 20, 2006
    Assignee: Bayer Healthcare AG
    Inventors: Thoams Krahn, Wolfgang Paffhausen, Andreas Schade, Martin Bechem, Delf Schmidt