Absorbent Column, Particles Or Resin Strip Patents (Class 436/541)
  • Patent number: 5460979
    Abstract: A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labelled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made.
    Type: Grant
    Filed: February 7, 1994
    Date of Patent: October 24, 1995
    Assignee: Becton Dickinson and Company
    Inventors: Robert A. Levine, Stephen C. Wardlaw, Leon W. M. M. Terstappen, Thomas J. Mercolino, Diether J. Recktenwald
  • Patent number: 5460940
    Abstract: Methods and cards for detecting an antigen or antibody in a fluid sample are disclosed. The fluid sample is added to a microreaction vessel containing a slurry suspension of inert particles and a binding partner to the antigen or antibody to be determined. The fluid sample is added to the micro reaction vessel, with one of the antigen and antibody binding partners being carrier bound. The vessel contents are centrifuged, to cause the binding partners to contact each other to form an optically detectable binding complex. The location of the optically detectable complex is observed to determine the presence of the antibody.
    Type: Grant
    Filed: January 4, 1995
    Date of Patent: October 24, 1995
    Assignee: Stiftung fur diagnostische Forschung
    Inventors: Lapierre Yves, Dieter Josef, Jean Adam, Susanne Greber-Widmer
  • Patent number: 5459080
    Abstract: The present invention includes novel assays employing a capture reagent, involving a first specific binding member conjugated to a polymeric anion such as carboxymethylamylose, and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to/ the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of carboxymethylamylose to prepare a suitably charged capture reagent provides a superior capture reagent that is capable of binding and retaining the analyte on the solid phase even in the presence of polyanionic non-specific binding blockers.
    Type: Grant
    Filed: January 27, 1994
    Date of Patent: October 17, 1995
    Assignee: Abbott Laboratories
    Inventors: Janina Adamczyk, Daniel S. Berry, Yi-Her Jou, Stephen D. Stroupe
  • Patent number: 5459078
    Abstract: The present invention includes novel digoxin assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two percent. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex.
    Type: Grant
    Filed: June 9, 1993
    Date of Patent: October 17, 1995
    Assignee: Abbott Laboratories
    Inventors: Steven Kline, Yi-Her Jou, Stephen D. Stroupe, Janina Adamczyk, Daniel S. Berry, Rosario M. Fico, James J. Markese
  • Patent number: 5447870
    Abstract: Disclosed is a primary chromatographic support containing an immobilized flocculating agent for use as a separation media for a secondary support consisting of microparticles used in affinity separation and heterogeneous immunoassays. In a specific embodiment, a flocculating agent such as polyethyleneimine is immobilized on a chromatographic resin packed in a column. The column so formed is then used to trap microparticles having an affinity ligand or binder for the ligand affixed thereon. Such microparticles are used as a solid support for the affinity reaction involved in a variety of immunoassay formats.
    Type: Grant
    Filed: October 29, 1993
    Date of Patent: September 5, 1995
    Assignee: E. I. Du Pont de Nemours and Company
    Inventor: Hon-Peng P. Lau
  • Patent number: 5441896
    Abstract: The present invention is directed to a new assay procedure and improved solid phase assay devices for use with a so-called physical developer. The invention provides a new assay procedure for detecting the presence of an analyte in a fluid sample using a tracer having a metal label which signal is enhanced by applying a physical developer. The assay is performed with a device having a support for a test area and having attached thereto a binder specific for the analyte to be detected. The assay comprises the steps of directing the fluid sample and the tracer through a porous medium into contact with the test area at a controlled flow rate to allow binding of the analyte with the binder and the tracer to the analyte, directing a physical developer through said porous medium to amplify the signal generated by the tracer, and separating the device to allow the test area to be read. The invention further relates to devices and immunoassay test kits for carrying out the improved immunoassay procedure.
    Type: Grant
    Filed: June 8, 1993
    Date of Patent: August 15, 1995
    Inventors: Marcus J. M. Noppe, Theo C. Garrevoet
  • Patent number: 5437983
    Abstract: A method for carrying out a binding assay is described wherein a member of a specific binding pair (sbp) and a sample are combined with a matrix of non-porous beads in a liquid medium under conditions such that the beads bind to the sbp member. The liquid medium is removed from the beads by aspiration using an aspiration tube having one or more orifices each of a diameter smaller than the minimum diameter of the smallest bead thereby allowing removal of the liquid medium while prohibiting aspiration of the beads.
    Type: Grant
    Filed: February 1, 1993
    Date of Patent: August 1, 1995
    Assignee: Syntex (U.S.A.) Inc.
    Inventors: Richard P. Watts, Mary C. Ericson, Hrair Kirakossian, Chiu C. Chang
  • Patent number: 5434088
    Abstract: A target substance is detected by a sandwich immunoassay using fine particle (A) having bound to it a fluorescer and an antibody reacting specifically with the target substance, and a fine particle (B) having bound to it a quencher and an antibody reacting specifically with the target substance, through a different antigenic determinant. Also disclosed is a competitive immunoassay having a fine particle (C) bound to it a fluorescer or a quencher, and an antibody reacting specifically with the target substance, a bound product (D) composed of the remainder of the fluorescer and the quencher, or a known amount of the target substance. Binding of the fluorescer and the quencher to the fine particle (A), (B) or (C) is affected so that the fluorescer and the quencher are covalently bound to a substance adsorbed on the fine particle.
    Type: Grant
    Filed: May 24, 1994
    Date of Patent: July 18, 1995
    Assignee: Tosoh Corporation
    Inventors: Kenji Ikeda, Kazuhisa Toyoda, Hideo Suzuki
  • Patent number: 5427959
    Abstract: On a slab-like board formed of a transparent material is closely attached a wedge-shaped transparent cover member provided with a recess in a central inner portion, thereby to form a clearance. The height of the clearance between the recess and the board is configured to decrease continuously or in steps. When an immunological active substance such as a monoclonal antibody is caused to sensitize carrier particles F, and a reagent having the carrier particles F dispersed into a liquid medium mainly composed of the water is mixed with a specimen, the reaction will occur in which the flocculate is formed from plural carrier particles. When this reaction liquid is poured into the clearance through the opening, the reaction liquid penetrates in the direction having a narrower vertical spacing due to surface tension. A single carrier particle unflocculated can move deep within the recess because it is small in diameter, but the flocculate G is trapped on its way and can not move because of its size.
    Type: Grant
    Filed: October 1, 1991
    Date of Patent: June 27, 1995
    Assignee: Canon Kabushiki Kaisha
    Inventors: Matsuomi Nishimura, Kazumi Tanaka, Takeshi Miyazaki, Hidehito Takayama, Toshikazu Ohnishi
  • Patent number: 5422239
    Abstract: Hybridomally produced monoclonal IgM antibodies having high affinity are useful for the immunoassay and purification of vital antigens.
    Type: Grant
    Filed: May 15, 1987
    Date of Patent: June 6, 1995
    Assignee: General Hospital Corporation
    Inventors: Jack R. Wands, Vincent R. Zurawski, Jr.
  • Patent number: 5422284
    Abstract: Disclosed is a primary chromatographic support containing an immobilized flocculating agent for use as a separation media for a secondary support consisting of microparticles used in affinity separation and heterogeneous immunoassays. In a specific embodiment, a flocculating agent such as polyethyleneimine is immobilized on a chromatographic resin packed in a column. The column so formed is then used to trap microparticles having an affinity ligand or binder for the ligand affixed thereon. Such microparticles are used as a solid support for the affinity reaction involved in a variety of immunoassay formats.
    Type: Grant
    Filed: October 29, 1993
    Date of Patent: June 6, 1995
    Assignee: E. I. Du Pont de Nemours and Company
    Inventor: Hon-Peng P. Lau
  • Patent number: 5418171
    Abstract: The present invention relates to an apparatus for determining the presence or absence of a target analyte in a liquid sample, comprising:(i) a container capable of accommodating the liquid sample and having a transparent portion; and(ii) an insertion member which is capable of being inserted into the container comprising;a porous member which has a main surface and a reverse surface and which has on the main surface a substance capable of specifically binding to the target analyte; andan absorbent bonded to the reverse surface of the porous member;the porous member being supported in the insertion member whereby, when the insertion member is inserted into the container, the main surface can be observed from the outside of the container through the transparent portion of the vessel and the liquid sample is absorbed into the absorbent through the porous member.According to the analyzer of the present invention, the presence or absence of a target analyte in a sample can be easily determined.
    Type: Grant
    Filed: November 30, 1992
    Date of Patent: May 23, 1995
    Assignee: Meiji Seika Kabushiki Kaisha
    Inventors: Fumio Kimura, Naohisa Koizumi, Koichi Matsuo, Minoru Aoyagi, Kiyomi Harakawa
  • Patent number: 5407836
    Abstract: The present invention provides a process for the determination of low density lipoproteins (LDL) in body fluids, wherein high density lipoprotein (HDL) antibodies are added to a sample to be investigated, insolubles formed are separated off and the LDL or one of its components is determined in the supernatant.The present invention also provides a reagent for the determination of the LDL fraction in body fluids, wherein it contains HDL antibodies.
    Type: Grant
    Filed: June 30, 1993
    Date of Patent: April 18, 1995
    Assignee: Boehringer Mannheim GmbH
    Inventors: Joachim Ziegenhorn, Sigbert Schiefer, Brigitte Drager
  • Patent number: 5405784
    Abstract: The invention provides a method capable of determining the presence or absence of each of a plurality of different ligands in a specimen. The specimen is contacted with a predetermined number of different homogeneous populations of fluorescence beads having one or more predetermined antiligands affixed to their surface. The specimen and bead mixture is analyzed using a means having a single parameter for measuring the fluorescence per ligand to determine the number of nonagglutinated beads, the number of agglutinated beads, the number of bead aggregates, and for each aggregate, the number of beads it comprises. This information is used to correlate the presence or absence in the specimen of each of the different ligands analyzed for. The method of the invention thus provides for the simultaneous determination of a predetermined number of ligands in a specimen using only a single bead contacting step.
    Type: Grant
    Filed: February 25, 1993
    Date of Patent: April 11, 1995
    Assignee: Chemunex
    Inventor: Michel Van Hoegaerden
  • Patent number: 5403745
    Abstract: A method is provided, in one embodiment, for the determination of an analyte in a biological fluid sample in the presence of a substance interfering with an assay for the analyte. This embodiment is implemented by using antibodies to cause the selective immunoreaction of at least one of the analyte or the interfering substance and then conducting an assay for the analyte in at least one of the immunoreactants or the non-reactants. Another embodiment provides a disposable reaction device to implement the method. The invention is applicable to the detection of a wide variety of analytes, including cholesterol in a targeted lipoprotein class in the presence of cholesterol in another class; to targeted isozymes of enzymes such as creatine kinase, lactate dehydrogenase, amylase, and alkaline or acid phosphatases in the presence of other isozymes; as well as to targeted immunoglobulins in the presence of non-targeted immunoglobulins.
    Type: Grant
    Filed: April 27, 1990
    Date of Patent: April 4, 1995
    Assignee: Genzyme Corporation
    Inventors: James F. Ollington, Ronald J. Byrnes, Donald E. Pogorzelski
  • Patent number: 5403714
    Abstract: A biologic sample such as feces, sputum, cervical tissue, pleural fluids, exudates, cytologic specimens, or the like, is tested for the presence or absence of: ova; parasites; microorganisms; inflammatory, neoplastic tissue cells; or other target materials which are indicative of infestation, disease or infection. The sample is mixed with a buffer fluid and placed in a transparent tube which contains a volume-constricting cylindrical insert for gravimetric separation of components of the sample. The mixture is centrifuged, and the annular space between the insert and tube bore is examined under magnification for the presence of the target materials.
    Type: Grant
    Filed: January 15, 1993
    Date of Patent: April 4, 1995
    Inventors: Robert A. Levine, Stephen C. Wardlaw, Paul N. Fiedler
  • Patent number: 5389523
    Abstract: A method of immunoanalysis combines immobilized immunochemistry with the technique of flow injection analysis, and employs microscopic spherical structures called liposomes, or lipid vesicles, as carriers of detectable reagents. Liposomes are modified on their surface with analytical reagents, and carry in their internal volume a very large number of fluorescent or electroactive molecules. Aspects of this embodiment of the invention include the chemistry for covalent immobilization of antibody fragments in a specified orientation, the use of liposomes in a flow injection analysis system, and the combination of automated sampling and analysis with reusable immunoreactants. Another aspect of the invention involves the non-covalent binding of liposomes to a receptor for use in a homogeneous assay. In another aspect of the invention the intensity of scattered light is quantitated as a measure of liposome aggregation in response to a concentration-dependent immunospecific reaction.
    Type: Grant
    Filed: July 23, 1992
    Date of Patent: February 14, 1995
    Assignee: The United States of Americas, as represented by the Secretary of Commerce
    Inventors: Anne L. Plant, Laurie Locascio-Brown, Richard A. Durst
  • Patent number: 5378624
    Abstract: A method is provided for removing a second ligand from a particle surface without substantially affecting the particle surface, comprising the step of exposing the particle to a first ligand immobilized onto a support, wherein the particle is exposed under conditions and for a residence time sufficient to allow the second ligand to desorb from the particle surface, and wherein the first ligand has an affinity for the second ligand that is at least two orders of magnitude greater than the affinity of the second ligand for the particle surface, such that the second ligand is removed from the particle surface without substantially affecting the particle surface.
    Type: Grant
    Filed: April 23, 1990
    Date of Patent: January 3, 1995
    Assignee: CellPro, Incorporated
    Inventors: Ronald J. Berenson, Dale R. Peterson
  • Patent number: 5376557
    Abstract: The present invention provides a method for type determination in body fluids of antibodies of a definite immunoglobulin class which are directed against an antigen A according to the immunoassay principle by incubation of the sample solution with at least two receptors R.sup.1 and R.sup.2, of which R.sup.1 is immobilizable and binds with the antibody to be determined and R.sup.2 is labelled and binds either with the antibody to be determined or with the antigen A, separation of the solid phase from the liquid phase and measurement of the label in one of the two phases, wherein the immunological reaction is carried out in homogeneous phase and the sample solution is incubated simultaneously with receptor R.sup.1 and an excess of human antibodies or fragments thereof which belong to the same immunoglobulin class as the antibody to be determined and are not bindable with the antigen A and subsequently receptor R.sup.2 and possibly further receptors are added thereto.
    Type: Grant
    Filed: July 30, 1992
    Date of Patent: December 27, 1994
    Assignee: Boehringer Mannheim GmbH
    Inventor: Urban Schmitt
  • Patent number: 5376556
    Abstract: A method, composition, device, apparatus, and kit for the determination of the presence or amount of an analyte by monitoring an analyte-mediated ligand binding event in a test mixture which contains the analyte to be assayed, a specific binding member, a Raman-active label, and a particulate having a surface capable of inducing a surface-enhanced Raman light scattering. The test mixture is illuminated with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum. The differences in the detected surface-enhanced Raman scattering spectra are dependent upon the amount of the analyte present in the test mixture. Thus, by monitoring these differences, the presence or amount of the analyte are determined.
    Type: Grant
    Filed: September 11, 1992
    Date of Patent: December 27, 1994
    Assignee: Abbott Laboratories
    Inventors: Peter J. Tarcha, Thomas E. Rohr, James J. Markese, Therese Cotton, Bernard N. Rospendowski
  • Patent number: 5374516
    Abstract: Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.
    Type: Grant
    Filed: February 24, 1989
    Date of Patent: December 20, 1994
    Assignee: Eastman Kodak Company
    Inventors: Richard C. Sutton, Brent A. Burdick, Susan J. Danielson, Harold C. Warren, III, Brian A. Snyder, Gregory J. McClune, Annie L. Wu
  • Patent number: 5354655
    Abstract: There is disclosed a process and a device for detecting and measuring (1) the amount of enzyme present as a detecting system following a nucleic acid hybridization reaction or immunoreaction; (2) the level and activity of free enzyme in a biological sample; (3) the level of enzyme from contaminating microorganisms present in a sample; and (4) enzymes from pure culture isolates for microbial identification and antimicrobial susceptibility testing.
    Type: Grant
    Filed: March 29, 1988
    Date of Patent: October 11, 1994
    Assignee: BioControl Systems, Inc.
    Inventors: N. Robert Ward, Jr., Philip J. Lozier
  • Patent number: 5352616
    Abstract: The present invention provides a method of assessment of the concentration of a subset of variants in a population of proteinaceous analyte variants capable of separation by a fractionation system, wherein said population of variants are contacted with a population of labelled proteinaceous specific binding partners therefor to form labelled binding partner-analyte complexes therewith, which are then subjected to separation by the said fractionation system into one or more fractions containing said subset of analyte variants in complexed form and assessment of the amount of label in one or more fractions so obtained, the said population of specific binding partners having, prior to reaction, substantially uniform distribution or mobility in said fractionation system, compositions and test kits for use in such methods. The method of the invention is particularly suitable for the analysis of variants of the protein transferrin.
    Type: Grant
    Filed: February 19, 1993
    Date of Patent: October 4, 1994
    Assignee: Axis Biochemicals AS
    Inventor: Erling Sundrehagen
  • Patent number: 5342790
    Abstract: A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labeled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made.
    Type: Grant
    Filed: October 30, 1992
    Date of Patent: August 30, 1994
    Assignee: Becton Dickinson and Company
    Inventors: Robert A. Levine, Stephen C. Wardlaw, Leon W. M. M. Terstappen, Thomas J. Mercolino, Diether J. Recktenwald
  • Patent number: 5338689
    Abstract: A method of detecting target antibodies or antigens by reaction with specific binding partners thereto is disclosed. One of the target or the binding partner is bound to a carrier, and the other is unbound. The complex between the target and the binding partner, with one being carrier-bound, forms an optically detectable binding complex. A microreaction vessel having an upper portion, a transition portion and a lower portion is utilized, wherein the upper portion has a greater diameter or width than the lower portion, and the transition portion is situated between the upper portion and the lower portion, and is funnel shaped. The microreaction vessel contains a slurry or suspension of inert particles, and unbound target or binding partner thereto. A solution of the carrier bound target or binding partner thereto is added to the vessel, which is then centrifuged to produce an optical determination of the target.
    Type: Grant
    Filed: October 30, 1992
    Date of Patent: August 16, 1994
    Assignee: Stiftung fur diagnostische Forschung
    Inventors: Lapierre Yves, Dieter Josef, Jean Adam, Susanne Greber-Widmer
  • Patent number: 5336597
    Abstract: The present invention provides a method for assaying a sample of cells for the presence of a cell surface antigen. More particularly, the present invention describes methods for detecting the presence of plasma and cell bound autoantibodies against cell surface antigens. In addition, the present invention provides a method of crossmatching donor platelets and transfusion recipients.
    Type: Grant
    Filed: October 28, 1992
    Date of Patent: August 9, 1994
    Assignee: The Scripps Research Institute
    Inventor: Robert McMillan
  • Patent number: 5308749
    Abstract: Biologically active reagents are prepared from particles of copolymers having highly active succinimid groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through amide groups by displacement of highly active succinimid groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods such as affinity chromatography.
    Type: Grant
    Filed: January 25, 1991
    Date of Patent: May 3, 1994
    Assignee: Eastman Kodak Company
    Inventors: Richard C. Sutton, Ignazio S. Ponticello, Susan J. Danielson, Marsha D. B. Oenick
  • Patent number: 5302532
    Abstract: Disclosed is a primary chromatographic support containing an immobilized flocculating agent for use as a separation media for a secondary support consisting of microparticles used in affinity separation and heterogeneous immunoassays. In a specific embodiment, a flocculating agent such as polyethyleneimine is immobilized on a chromatographic resin packed in a column. The column so formed is then used to trap microparticles having an affinity ligand or binder for the ligand affixed thereon. Such microparticles are used as a solid support for the affinity reaction involved in a variety of immunoassay formats.
    Type: Grant
    Filed: April 14, 1989
    Date of Patent: April 12, 1994
    Assignee: E. I. Du Pont de Nemours and Company
    Inventor: Hon-Peng P. Lau
  • Patent number: 5296356
    Abstract: The invention concerns a method for the detection of an analyte in a sample liquid by an enzyme-immunoassay in which an enzyme-labelled compound is partitioned between a solid and a liquid phase and the amount of enzyme label in the liquid phase outside the solid phase is determined as a measure of the concentration of the analyte. The measurement is carried out in a non-porous molding having the liquid phase contained therein in contact with the solid phase. The method is particularly suitable for carrying out in a cuvette which can be filled via the porous matrix or on a test strip having a space in contact with the solid phase.
    Type: Grant
    Filed: December 12, 1990
    Date of Patent: March 22, 1994
    Assignee: Boehringer Mannheim GmbH
    Inventors: Dieter Mangold, Ulrich Traeger, Hans Lange
  • Patent number: 5290707
    Abstract: A microorganism detection system provides initial warning, confirmation of dentity, and recognition of pathogenic factors in microorganisms from environmental samples. The method and apparatus of the invention uses different sized antibody coated microspheres which react with unknown antigens, are sized by electronic volume sizing, and are sorted by size. The sized particles are quantitated in addition to being sized. The microsphere sizes indicate the presence of specific microorganism groups.The samples can be further analyzed using fluorescent microspheres which agglutinate with the sized microspheres. The presence of specific microorganisms is indicated by a change in the fluorescence of the sample.
    Type: Grant
    Filed: November 25, 1991
    Date of Patent: March 1, 1994
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventor: Sheila J. Wood
  • Patent number: 5275789
    Abstract: Materials are labelled with a radioisotope by passing them through a column packed with (a) beads coated with an oxidizing reagent for coupling the radioisotopes to the materials, (b) as anion resin, and (c) a material for trapping elemental isotope, and flowing a mixture of the radioisotope and a solution of the material to be labelled through the column.
    Type: Grant
    Filed: June 17, 1992
    Date of Patent: January 4, 1994
    Assignee: E. I. Du Pont de Nemours and Company
    Inventor: William R. Taylor
  • Patent number: 5270169
    Abstract: The invention provides for methods and compositions to detect the presence of anti-HLA antibodies, HLA antigen or preformed HLA-containing immune complexes in biological samples. The complement protein Clq is bound to a solid substrate, then mixed with a biological sample containing immune complexes. The immune complexes are preformed, or formed by adding HLA antigens to a biological sample containing antibodies to HLA, or alternatively, by combining a biological sample containing HLA antigens with defined antibodies to HLA. The immune complexes bind to Clq, and are then detected by the addition of a labeled reagent.
    Type: Grant
    Filed: June 23, 1992
    Date of Patent: December 14, 1993
    Assignee: SangStat Medical Corporation
    Inventors: Chin-Hai Chang, Philippe Pouletty
  • Patent number: 5262334
    Abstract: The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed.
    Type: Grant
    Filed: October 3, 1991
    Date of Patent: November 16, 1993
    Assignee: Fred Hutchinson Cancer Research Center
    Inventors: Ronald J. Berenson, William I. Bensinger
  • Patent number: 5262297
    Abstract: Specific binding methods are used for diagnostic assays and purification separations whereby the specific binding capture reagent is prepared from copolymers having highly reactive carboxy groups. These groups are extended from the polymer surface with a linking group having from 8 to 50 atoms in the chain and two or more alkylene, arylene, alkylenearylene or arylenealkylene groups. To these reactive groups is attached a biologically active substance such as a protein or oligonucleotide which then participates in the diagnostic assays or purification separation methods.
    Type: Grant
    Filed: April 30, 1992
    Date of Patent: November 16, 1993
    Assignee: Eastman Kodak Company
    Inventors: Richard C. Sutton, Susan J. Danielson, John B. Findlay, Fred. T. Oakes, Marsha D. B. Oenick, Ignazio S. Ponticello, Harold C. Warren, III
  • Patent number: 5260193
    Abstract: A method and device for determining the presence of an analyte in a sample suspected of containing the analyte is disclosed. The method involves contacting a test solution containing the sample, an antibody for the analyte, and a conjugate of the analyte and a label with a contact portion of a strip of bibulous material capable of being traversed by the test solution through capillary action. The strip contains a first receptor capable of binding to said conjugate. The first receptor is non-diffusively bound to a situs on the strip separate from the contact portion of the strip. The strip further contains a second receptor capable of binding the antibody to the analyte between the situs and the contact portion. The second receptor is non-diffusively bound to the strip. At least a portion of the test solution is allowed to traverse the strip by capillary action and thereby contact the situs.
    Type: Grant
    Filed: November 5, 1991
    Date of Patent: November 9, 1993
    Assignee: Syntex (U.S.A.) Inc.
    Inventor: John D. Olson
  • Patent number: 5260194
    Abstract: A method and device for determining the presence of an analyte in a sample suspected of containing the analyte is disclosed. The method involves contacting a test solution containing the sample, an antibody for the analyte, and a conjugate of the analyte and a label with a contact portion of a piece of bibulous material capable of being traversed in at least one direction by the test solution through capillary action. The bibulous material contains a first receptor capable of binding to said conjugate. The first receptor is non-diffusively bound to a situs on the bibulous material separate from the contact portion. The bibulous material further contains a second receptor capable of binding the antibody to the analyte between the situs and the contact portion. The second receptor is non-diffusively bound to the bibulous material. At least a portion of the test solution is allowed to traverse the bibulous material by capillary action and thereby contact the situs.
    Type: Grant
    Filed: November 5, 1991
    Date of Patent: November 9, 1993
    Assignee: Syntex (U.S.A.) Inc.
    Inventor: John D. Olson
  • Patent number: 5252460
    Abstract: A biologic sample such as feces, sputum, cervical tissue, pleural fluids, exudates, cytologic specimens, or the like, is tested for the presence or absence of: ova; parasites; microorganisms; inflammatory, neoplastic tissue cells; or other target materials which are indicative of infestation, disease or infection. The sample is mixed with a buffer fluid and placed in a transparent tube which contains a volume-constricting cylindrical insert for gravimetric separation of components of the sample. The mixture is centrifuged, and the annular space between the insert and tube bore is examined under magnification for the presence of the target materials.
    Type: Grant
    Filed: October 28, 1991
    Date of Patent: October 12, 1993
    Inventors: Paul N. Fiedler, Stephen C. Wardlaw, Robert A. Levine
  • Patent number: 5242837
    Abstract: The present invention relates to an optical apparatus and a uniaxial method for rapidly measuring spectroscopically labelled specific binding analytes in a reaction assay mixture that contains unbound label without requiring the physical separation of the unbound label from the reaction mixture or sequential reagant additions and incubations. Moreover, the technique is equally applicable to measurements in either serum or whole blood.
    Type: Grant
    Filed: December 24, 1990
    Date of Patent: September 7, 1993
    Inventors: Rudolf E. Slovacek, Michael A. Harvey
  • Patent number: 5231035
    Abstract: Methods for determining the presence of a first ligand, preferably a hapten, in a sample suspected to contain the first ligand are provided, along with reagent systems and apparatus suitable for performing the methods. The methods depend upon a color visualization indicating the presence or absence of the first ligand in the sample. Preferred methods comprise contacting the sample with a reagent system which comprises: (1) colored particles which bear on their surface a second ligand which may be the same as or different than the first ligand; and (2) an amount of a receptor which is specific for the first ligand and the second ligand, wherein the amount is sufficient to stabilize the particles. The methods further comprise passing the contacted sample and reagent system through a filter, and then analyzing the color of the filtrate. The presence of ligand in the sample is established where the color of the filtrate is substantially different from the color of the ligand-bearing particles.
    Type: Grant
    Filed: January 11, 1991
    Date of Patent: July 27, 1993
    Assignee: Akers Research Corporation
    Inventor: Raymond F. Akers, Jr.
  • Patent number: 5229268
    Abstract: A method for performing a diagnostic immunoassay by a solid phase separation. To a reaction mixture of a test sample and labeled antibody, which forms a complex of any analyte present in the test sample, is added a solid phase material having a compound capable of binding any excess labeled antibody. The solid phase material is chosen to rapidly settle whereby a solid and liquid phase is formed. The liquid phase can then be extracted to measure the amount of analyte-labeled antibody present therein.
    Type: Grant
    Filed: November 24, 1987
    Date of Patent: July 20, 1993
    Assignee: Abbott Laboratories
    Inventors: Terry A. Pry, Edward N. Granados, Philip M. Hill
  • Patent number: 5225353
    Abstract: The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed.
    Type: Grant
    Filed: October 3, 1991
    Date of Patent: July 6, 1993
    Assignee: Fred Hutchinson Cancer Research Center
    Inventors: Ronald J. Berenson, William I. Bensinger
  • Patent number: 5215927
    Abstract: The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed.
    Type: Grant
    Filed: August 2, 1991
    Date of Patent: June 1, 1993
    Assignee: Fred Hutchinson Cancer Research Center
    Inventors: Ronald J. Berenson, William I. Bensinger
  • Patent number: 5206139
    Abstract: There is disclosed a process and a device for detecting and measuring (1) the amount of enzyme present as a detecting system following a nucleic acid hybridization reaction or immunoreaction; (2) the level and activity of free enzyme in a biological sample; (3) the level of enzyme from contaminating microorganisms present in a sample; and (4) enzymes from pure culture isolates for microbial identification and antimicrobial susceptibility testing.
    Type: Grant
    Filed: March 29, 1988
    Date of Patent: April 27, 1993
    Assignee: BioControl Systems, Inc.
    Inventors: Jr. Ward, Philip J. Lozier
  • Patent number: 5206177
    Abstract: An apparatus for determining an analyte in a sample, as well as a method for carrying this out, are disclosed. The apparatus utilizes a first zone containing a labeled substance, and a second zone which permits separation of a mobile detectable moiety from unreacted reaction component. Detectable moiety is the product of reaction between reaction component and labeled substance.
    Type: Grant
    Filed: September 21, 1990
    Date of Patent: April 27, 1993
    Assignee: Boehringer Mannheim Corporation
    Inventors: Fern DeLaCroix, Johann Berger, Harvey Buck, Hans-Joachim Guder
  • Patent number: 5198369
    Abstract: A mixture is prepared by mixing carriers to which a substance specifically reacting on an object substance adheres with a sample containing the object substance. The mixture is irradiated by light having an intensity gradient to concentrate the carriers at and near the irradiated positions by the optical pressure of the light. The efficiency of an agglomeration reaction of the carriers is thereby increased. A qualitative or quantitative measurement of the object substance is performed by detecting a state of agglomeration of the carriers within the mixture.
    Type: Grant
    Filed: April 19, 1991
    Date of Patent: March 30, 1993
    Assignee: Canon Kabushiki Kaisha
    Inventors: Yuuji Itoh, Michiyo Itoh, Yoshiyuki Touge, Atsushi Saitou, Tatsuya Yamazaki
  • Patent number: 5191068
    Abstract: A method of removing an antigenic substance from a fluid comprises(1) forming a ternary complex by the interaction of(a) the antigenic substance,(b) a first antibody which contains a kappa chain and which binds to the antigenic substance, and(c) a second antibody which binds to the kappa chain of the first antibody, said second antibody being immobilized on a solid phase carrier, and(2) separating the fluid from the solid phase carrier.
    Type: Grant
    Filed: September 4, 1990
    Date of Patent: March 2, 1993
    Assignee: Eastman Kodak Company
    Inventors: Alan R. Thomson, Frances L. Stickley, Stephen E. Clark, John L. Daiss
  • Patent number: 5183740
    Abstract: A method of detecting a target moiety comprising the steps of (a) providing an antibody specific to the target, (b) saturating the binding sites of the antibody with a labelled form of the target, (c) flowing a liquid containing the target past the saturated antibody, thereby (d) allowing the target to displace the labelled antigen, and (e) detecting the displaced labelled antigen with a detector for the label.
    Type: Grant
    Filed: February 23, 1990
    Date of Patent: February 2, 1993
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventors: Frances S. Ligler, Gaber Bruce P., Anne W. Kusterbeck, Gregory A. Wemhoff
  • Patent number: 5176998
    Abstract: The present invention provides a method for assaying a sample of cells for the presence of a cell surface antigen. More particularly, the present invention describes methods for detecting the presence of plasma and cell bound autoantibodies against cell surface antigens. In addition, the present invention provides a method of crossmatching donor platelets and transfusion recipients.
    Type: Grant
    Filed: November 24, 1987
    Date of Patent: January 5, 1993
    Assignee: The Scripps Research Institute
    Inventor: Robert McMillan
  • Patent number: RE34312
    Abstract: The present invention provides a process for the determination of an antibody by incubation with three different reagents R.sub.1, R.sub.2 and R.sub.3, of which R.sub. and R.sub.3 are present in liquid phase and are bindable with the antibody, R.sub.2 is present bound to a solid phase and is bindable with R.sub.1 and R.sub.3 carries a label, separation of the solid phase from the liquid phase and measurement of the label in the solid phase, wherein as R.sub.1 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a reaction component of a specific binding system, as R.sub.3 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a label and as R.sub.2 there used the other binding component of the specific binding system. The present invention also provides a composition for the determination of an antibody, wherein it contains two different soluble reagents R.sub.1 and R.sub.
    Type: Grant
    Filed: November 21, 1991
    Date of Patent: July 13, 1993
    Assignee: Boehringer Mannheim GmbH
    Inventors: Thomas Geiger, Wolf D. Engel, Urban Schmitt, Eberhard Maurer, Wolfgang Rudinger, Rolf Deeg
  • Patent number: RE34405
    Abstract: Methods and compositions are provided for concentrating particles in a minute area on a solid surface. The method permits the detection of small amounts of analytes by providing for an observable signal in relation to the concentration of particles in the area.
    Type: Grant
    Filed: November 12, 1987
    Date of Patent: October 12, 1993
    Assignee: Abbott Laboratories
    Inventors: Dennis R. Gould, Robert F. Zuk