Biological Cellular Material Tested Patents (Class 436/63)
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Patent number: 9797909Abstract: A sensor for detecting and/or quantifying the amount of analyte in a sample, the sensor including: a sensing region; and a barrier layer including a reactive oxygen species (ROS)-quenching, analyte-permeable membrane having an ROS-quenching agent adsorbed thereto; wherein the sensor is adapted so that the sample enters the sensing region of the sensor through the barrier layer.Type: GrantFiled: September 26, 2016Date of Patent: October 24, 2017Assignee: Lightship Medical LimitedInventors: William Paterson, Nick Barwell, Bruce Culbert, Barry Colin Crane
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Patent number: 9737260Abstract: The present invention is based on the finding that enrichment of D3-creatinine in a urine sample following oral administration of a single defined dose of D3-creatine can be used to calculate total-body creatine pool size and total body skeletal muscle mass in a subject. The invention further encompasses methods for detecting creatinine and D3-creatinine in a single sample. The methods of the invention find use, inter alia, in diagnosing disorders related to skeletal muscle mass, and in screening potential therapeutic agents to determine their effects on muscle mass.Type: GrantFiled: April 13, 2016Date of Patent: August 22, 2017Assignees: GLAXOSMITHKLINE LLC, THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Marc K. Hellerstein, William Evans
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Patent number: 9739658Abstract: The present invention relates to a cell peeling recognizing device and a cell peeling recognizing method. When a moving part (robot 6) moves culture vessel 5, imaging device 14 images an interior of culture vessel 5 a plurality of times as the cells move inside the culture vessel due to the moment of inertia, and recognizing part 10a recognizes the peeled state of cells C by comparing various sets of the captured imaging data. The peeled state of cells in the culture vessel can be determined with high precision.Type: GrantFiled: October 29, 2015Date of Patent: August 22, 2017Assignee: SHIBUYA CORPORATIONInventor: Daisuke Watanabe
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Patent number: 9694177Abstract: A device for electrically controlled of ions, comprises a first electrolyte; a first electrode, which is arranged in direct or indirect contact with the first electrolyte, an encapsulation; and an ion conductor, which is arranged to receive and/or deliver ions from/to the first electrolyte. The encapsulation is arranged to effectively enclose the first electrolyte, and the ion conductor is arranged to transport ions from/to an outside of the encapsulation.Type: GrantFiled: March 20, 2008Date of Patent: July 4, 2017Assignee: OBOE IPR ABInventors: Magnus Berggren, Joakim Isaksson, Edwin Jager, Peter Kjall, David Nilsson, Agneta Richter-Dahlfors, Daniel T. Simon, Klas Tybrandt, Barbara Canlon
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Patent number: 9664698Abstract: The present invention inter alia provides a method, and uses thereof, of predicting statin-induced muscle toxicity or its complications, such as myalgia, myopathy and rhabdomyolysis, by detecting the lipid concentrations or lipid ratios of a biological sample and comparing it to a control. This method has identified lipid markers that are more specific and sensitive in detecting these statin-induced muscle toxicity than the currently utilized clinical markers. Also provided is an antibody towards said lipids, and the use thereof for predicting, diagnosing, statin-induced muscle toxicity. The invention additionally relates to kits comprising lipids and/or an antibody thereto, for use in the prediction and/or diagnosis of statin-induced muscle toxicity.Type: GrantFiled: April 8, 2011Date of Patent: May 30, 2017Assignee: ZORA BIOSCIENCES OYInventors: Reijo Laaksonen, Kim Ekroos, Reini Hurme, Minna Janis, Riikka Katainen, Kirill Tarasov
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Patent number: 9528981Abstract: The invention proposes a novel therapeutic and diagnostic methodology based on the use of effector cells (for example CTL and NK cells) selected for being able to induce lysis in target cells. In particular, the invention teaches how to select strains of effector cells of the immune system according to their lytic properties. It also teaches how diagnostic procedures for checking the activity of therapeutic vaccines can be improved with respect to what shown. Finally, it shows how cell therapies used at present can be improved by using this approach.Type: GrantFiled: April 12, 2007Date of Patent: December 27, 2016Assignee: Menarini Silicon Biosystems S.p.A.Inventors: Roberto Gambari, Roberto Guerrieri
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Patent number: 9502227Abstract: A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope. The nanospray ionization capillary tip can include a filament in the interior. The method further includes capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, and performing the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip.Type: GrantFiled: February 28, 2014Date of Patent: November 22, 2016Assignee: HUMANIX CO., LTD.Inventors: Tsutomu Masujima, Naohiro Tsuyama, Hajime Mizuno
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Patent number: 9488621Abstract: The present invention is a method and apparatus for acoustically manipulating one or more particles.Type: GrantFiled: August 10, 2012Date of Patent: November 8, 2016Assignee: Los Alamos National Security, LLCInventors: Gregory Kaduchak, Michael D Ward
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Patent number: 9422588Abstract: An apparatus for measuring bacteria includes a sampling device for processing fluorescently stained bacteria, a detector for detecting size information from the bacteria in the sample, and a detector for detecting fluorescence information expressing intensity of fluorescent light emitted by the bacteria. The apparatus further includes a processor and memory that includes programs for creating a scattergram representing a distribution of the bacteria based on the size information and the fluorescence information detected, analyzing the distribution of the bacteria in the scattergram, and determining whether the bacteria in the sample is bacillus or coccus based on a result of the analysis.Type: GrantFiled: January 9, 2014Date of Patent: August 23, 2016Assignee: SYSMEX CORPORATIONInventor: Yasuyuki Kawashima
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Patent number: 9418825Abstract: A mass spectrometry is equipped with a liquid specimen supply part which supplies a liquid specimen sandwiched between bubbles, an ion source part ionizes the specimen, and a mass spectrometry part which detects ions separated in accordance with mass. In particular the ion source part is configured so as to include a liquid supply tube for transporting a specimen from the liquid specimen supply part, a degassing/liquid retention part in which bubbles are removed, a spraying part which ionizes the specimen, and a high-voltage power supply part which applies a high voltage to the spraying part. The device is further characterized in that after removing the bubbles, a Taylor cone is formed from the resultant pre-solution, and the specimen is ionized thereafter. Thus, the ionization of an intended specimen is stabilized, and the measurement reproducibility is improved.Type: GrantFiled: January 27, 2014Date of Patent: August 16, 2016Assignee: Hitachi High-Technologies CorporationInventors: Hiroyuki Yasuda, Shinya Ito, Akira Fujii, Yasushi Terui
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Patent number: 9404918Abstract: It is provided a method for detection and/or quantification of at least one molecule presents in blood by a MALDI-MS analysis of a dried fluid spot without the presence of any digestion step or liquid extraction step, which permits to further analyze the physical distribution of at least one molecule within a dried fluid spot.Type: GrantFiled: March 13, 2014Date of Patent: August 2, 2016Assignee: ZentechInventors: Maria D'Aloia, Nicolas Smargiasso, Delphine Debois, Edwin De Pauw
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Patent number: 9400236Abstract: Devices and methods for magnetophoretic analyte selection and concentration are described. Magnetically marked analytes (e.g., cells) may be separated out of a sample dynamically in flux, such that the magnetically marked analytes are present in a highly concentrated manner in a reduced sample volume. The analyte selection may be followed by an analysis.Type: GrantFiled: May 11, 2012Date of Patent: July 26, 2016Assignee: Siemens AktiengesellschaftInventor: Oliver Hayden
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Patent number: 9389162Abstract: A device and a method for detecting an analyte in a liquid sample. In an aspect, a mixing portion is configured to receive and mix a first liquid solution of unknown composition with a second liquid solution that includes a suspended particle resulting in a mixed liquid solution, wherein a surface of the suspended particle is functionalized to target an analyte. Furthermore, in an aspect, a surface of a solid substrate is configured to receive and evaporate a drop of the mixed liquid solution, wherein evaporation of the drop in connection with a capillary flow of the mixed liquid solution disperses the suspended particle in a ring pattern. The presence or absence of an analyte in the first liquid solution can be determined by visual inspection of the ring pattern.Type: GrantFiled: April 10, 2014Date of Patent: July 12, 2016Assignee: CITY UNIVERSITY OF HONG KONGInventors: Ting-Hsuan Chen, Wei Liu, Yuanhang Li
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Patent number: 9377390Abstract: A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.Type: GrantFiled: April 10, 2015Date of Patent: June 28, 2016Assignee: Inguran, LLCInventors: Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
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Methods and systems for measuring and using the oxidation-reduction potential of a biological sample
Patent number: 9360446Abstract: Methods and systems for measuring and using the oxidation-reduction characteristics of a biological sample are provided. The system generally includes a test strip and a readout device. A fluid sample is placed in the test strip, and the test strip is in turn operatively connected to the readout device. The readout device provides a controlled current that is sent across the fluid in the sample chamber. In addition, the readout device identifies an inflection point or transition time at which the voltage between contacts of the test strip is changing at the highest rate. The oxidation-reduction capacity of the sample is taken as the integral of the current profile from the time at which current begins to be supplied to the sample to the identified transition time.Type: GrantFiled: October 23, 2013Date of Patent: June 7, 2016Assignee: Aytu BioScience, Inc.Inventors: Raphael Bar-Or, David Bar-Or, Leonard T. Rael -
Patent number: 9347885Abstract: The method of detecting thalassemia by optical analysis of blood components is a spectral detection method that is based on the fluorescence spectra of a set of biomolecules, including tyrosine, tryptophan, nicotinamide adenine dinucleotide, and flavin adenine dinucleotide, which are all found in blood plasma, and porphyrin, which is found in red blood cells (RBCs). Measured ratios of intensity maxima between tryptophan and nicotinamide adenine dinucleotide, flavin adenine dinucleotide and nicotinamide adenine dinucleotide, tyrosine and tryptophan, and the normal form of porphyrin and the basic form of porphyrin may each be used, alone or in combination, to diagnose a patient as suffering from thalassemia.Type: GrantFiled: May 7, 2013Date of Patent: May 24, 2016Assignee: KING SAUD UNIVERSITYInventors: Mohamad Saleh Al Salhi, Vadivel Masilamani, Farjah Hassan Algahtani
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Patent number: 9315848Abstract: The present invention provides methods of identifying a genetic abnormality such as mutation in EGFR or KRAS or ALK which is associated with the management of lung cancer or diagnosing, prognosing or monitoring the treatment of pre-cancerous conditions of the lung, such as bronchial dysplasia or atypical alveolar hyperplasia (AAH), through the detection of at least one volatile organic compound indicative of these states.Type: GrantFiled: August 17, 2011Date of Patent: April 19, 2016Assignees: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD., THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATEInventors: Hossam Haick, Nir Peled
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Patent number: 9304139Abstract: An object of the present invention is to provide a method for analyzing hemoglobins which can accurately separate hemoglobins in a short time by liquid chromatography. The method for analyzing hemoglobins by liquid chromatography includes pretreating a sample with an oxidant and a binder for trivalent heme iron.Type: GrantFiled: October 7, 2014Date of Patent: April 5, 2016Assignee: SEKISUI MEDICAL CO., LTD.Inventors: Hiroaki Taira, Takayuki Oka
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Patent number: 9278352Abstract: A microfluidic arrangement including a substrate in which a microfluidic structure having several adjacent channels and at least one common supply line, into which the adjacent channels merge, is formed. Each of the adjacent channels form the cylinder of a cylinder-piston arrangement for receiving an associated piston. A method for producing the type of microfluidic-arrangement is also disclosed.Type: GrantFiled: September 27, 2011Date of Patent: March 8, 2016Assignee: FRAUNHOFER-GESELLSCHAFT ZUR FORDERUNG DER ANGEWANDTEN FORSHUNG E.V.Inventors: Klaus-Stefan Drese, Daniel Latta, Marion Ritzi-Lehnert, Tina Roeser
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Patent number: 9234238Abstract: Compositions and methods are disclosed that exploit the unprecedented modification of double-stranded DNA virus DNA-packaging motor protein connector polypeptides to render them capable of stable incorporation into lipid membranes as a self-assembled homodocamer that forms an aperture through which conductance can occur when an electrical potential is applied across the membrane. The aperture permits use of the modified protein as a biosensor, for dsDNA sequencing, SNP detection and highly sensitive affinity capture and fingerprinting of analytes, and also finds use in electropotential-driven solute translocation, such as for liposomal loading to form therapeutic nanoparticles (e.g., gene delivery) and bioreactors, and for other uses. The aperture can further be used in optical detection of dsDNA or other acceptor labeled analytes in a fluorophore donor labeled single pore channel.Type: GrantFiled: April 26, 2011Date of Patent: January 12, 2016Inventor: Peixuan Guo
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Patent number: 9222934Abstract: The present invention provides a reagent for analysis of immature leukocytes comprising: a surfactant which can damage cell membranes of erythrocytes and mature leukocytes, a solubilizing agent which can shrink the damaged blood cells and at least one dye for staining nucleic acid selected from the dyes of the formulae (I) and (II), wherein X? is an anion.Type: GrantFiled: August 1, 2013Date of Patent: December 29, 2015Assignee: SYSMEX CORPORATIONInventors: Yukiko Kataoka, Tomohiro Tsuji, Shinichiro Oguni, Ayumu Yoshida, Masaki Abe
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Patent number: 9196036Abstract: A device for determining objects in a color recording has an identifier, a feature extractor and a classifier. The identifier is implemented to identify the connected regions whose size or shape correspond to a predetermined condition from a plurality of connected regions existing in a binary image derived from a color recording based on a size or a shape of these connected regions. The feature extractor is implemented, for each of the identified connected regions, to extract a feature set from the color recording. The classifier is implemented to classify the identified connected regions into at least two disjunct groups based on the extracted feature sets for the identified connected regions.Type: GrantFiled: June 20, 2013Date of Patent: November 24, 2015Assignee: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.Inventors: Matthias Elter, Thorsten Zerfass, Dirk Steckhan, Thomas Wittenberg, Christian Muenzenmayer, Timo Schlarb
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Patent number: 9162229Abstract: Disclosed is a collection sled for collecting a waste droplet stream 122 in a waste stream catcher that is located below sample collectors. Contamination of collected samples is reduced by collecting the waste droplet stream at a position that is below the sample collectors. A waste trough is provided that gets progressively larger in a downward direction, which prevents backsplash of waste fluid from the collection cavity. Standard sample collector tubes, as well as 8-well strips and microscope slides can be used with the collection sled to collect sample particles.Type: GrantFiled: June 21, 2013Date of Patent: October 20, 2015Assignee: Bio-Rad Laboratories, Inc.Inventors: Nathan Michael Gaskill-Fox, Daniel N. Fox, Rodney C. Harris
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Patent number: 9140688Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: GrantFiled: September 28, 2012Date of Patent: September 22, 2015Assignee: Inguran, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Patent number: 9133009Abstract: The invention relates to a device (1) for forming droplets in a microfluidic circuit, the device comprising a chamber (3) containing a first fluid and defined by two opposite walls (10, 11) that diverge relative to each other in at least one given direction, and a microchannel (8) containing a second fluid and leading into a zone of said chamber (3) that is upstream relative to the given direction, the outlet of the microchannel (8) into the chamber (3) constituting an enlargement in the flow section for the second fluid, and the enlargement giving rise to droplets (14) of the second fluid forming within the first fluid.Type: GrantFiled: March 28, 2011Date of Patent: September 15, 2015Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, ECOLE POLYTECHNIQUEInventors: Charles Baroud, Rémi Dangla
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Patent number: 9128069Abstract: Analysis system and computer implemented method for analyzing biological samples are disclosed. The system has at least one analyzer for performing an analysis and a decision unit being operable to determine in response to the receipt of the analysis request whether results obtained from performing the analysis on the sample indicated in the analysis request are valid. This determination is executed by retrieving the meta information assigned to the sample and by applying the at least one condition on the meta information and wherein the at least one applied condition has at least a condition on whether the sample allows a valid analysis on the sample, and wherein the decision unit returns the decision that the analysis exercised on the indicated sample will return a valid result in case the conditions of the condition set are met by the sample.Type: GrantFiled: December 22, 2010Date of Patent: September 8, 2015Assignee: Roche Diagnostics Operations, Inc.Inventor: Lars Oeltjen
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Patent number: 9126895Abstract: The present invention provides a reagent for measuring a degree of oxidative stress that includes a compound represented by the following general formula (I) or a salt thereof, and a method of measuring a degree of oxidative stress using the reagent for measuring a degree of oxidative stress. In the general formula (I), R1, R2, R3 and R4 each independently represent a hydrogen atom, an ethyl group, an isopropyl group, a hydroxyalkyl group that has 1 to 4 carbon atoms, or a phenyl group, and at least one of R1, R2, R3 or R4 represents an isopropyl group, a hydroxyalkyl group having 1 to 4 carbon atoms, or a phenyl group.Type: GrantFiled: December 22, 2013Date of Patent: September 8, 2015Assignee: ARKRAY, Inc.Inventors: Kazuhiro Omiya, Toshihiro Imai, Emi Ashibe
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Patent number: 9110029Abstract: Provided herein are compositions, kits, methods and devices for cyanide detection, particularly for cyanide detection in biological samples such as whole blood. The method comprises (1) contacting a sample with a cobinamide conjugate comprising a cobinamide moiety and a carrier; and (2) measuring the absorbance of light by the cobinamide conjugate. The present disclosure provides field-deployable cyanide detection methods, compositions, kits and devices, which provide rapid, accurate readout at the point of contact. Further provided herein is a method for determining exposure of a subject to cyanide.Type: GrantFiled: March 14, 2013Date of Patent: August 18, 2015Assignee: Diagnostic Consulting Network, Inc.Inventors: Brendan O'Farrell, Hans Boehringer, Roy Chung, Winnie Tong
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Patent number: 9103757Abstract: The present invention generally discloses an extraction system that provides a locale for fluid processing and extraction on a post-microcapture transfer film. The extraction system includes a transfer film carrier and an extraction device forming a reservoir. The extraction system selectively excludes regions of the transfer film from the reservoir to advantageously reduce contamination due to matter adhered to the transfer film by non-specific transfer.Type: GrantFiled: August 13, 2010Date of Patent: August 11, 2015Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Thomas M. Baer, Robert H. Reamey, Keith E. Moravick, David F. Head, Bruce J. Richardson, Derrick A. Richardson
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Patent number: 9099543Abstract: A nanowire sensor having a nanowire in a network structure includes: source and drain electrodes formed over a substrate; a nanowire formed between the source and drain electrodes and having a network structure in which patterns of intersections are repeated; and a detection material fixed to the nanowire and selectively reacting with a target material introduced from outside.Type: GrantFiled: March 19, 2012Date of Patent: August 4, 2015Assignee: POSTECH ACADEMY-INDUSTRY FOUNDATIONInventors: Jeong Soo Lee, Yoon Ha Jeong, Tai Uk Rim, Chang Ki Baek, Sung Ho Kim, Ki Hyun Kim
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Patent number: 9086409Abstract: The present invention is about a microfluidic chip for rapid detection of different target proteins and a method for using the same. The microfluidic chip utilizes antibody-conjugated magnetic beads to bind to the target proteins to form a magnetic complex, and then use the signal labeled-antibodies that can recognize said magnetic complex. Purifying said magnetic complex by the micro-magnetic field on biochip, and introducing said purified magnetic complex into the fluorescent detection area on the chip to detect the amount of the target protein in said purified complex immediately.Type: GrantFiled: March 9, 2010Date of Patent: July 21, 2015Assignee: NATIONAL CHENG KUNG UNIVERSITYInventors: Gwo-Bin Lee, Huan-Yao Lei, Yu-Fang Lee, Kang-Yi Lien
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Patent number: 9074977Abstract: An analytical device such as a flow cytometer is provided in which a fluid sample flowing through a channel is focused into multiple, parallel particle streams by an acoustic wave field extending across the channel. Each stream is then presented to an individual detector to allow for simultaneous interrogation of the multiple streams and thus, high-throughput analysis of the fluid sample.Type: GrantFiled: July 24, 2014Date of Patent: July 7, 2015Assignee: STC.UNMInventors: Steven W Graves, Robert W. Applegate, Jr., Gabriel P. Lopez, Menake Eranda Piyasena
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Patent number: 9046540Abstract: A novel non-enzymatic assay for lipid modulating drugs is being described for biological samples. For the first time non-enzymatic mechanism of statin drugs in modulating lipid aggregates and forming lipid particles is being shown. A simple flow cytometer based testing for detection of lipid particles, the effect of lipid modulating drug such as statin in vitro and testing for efficacy of the drug for an individual, dosage adjustment and drug discovery mechanism is being described. The lipid modulating drugs either individually or in combination can be discovered, optimized or efficacy tested using this assay. A novel statin induced lipid particle formation described can be used for diagnosis, personalized medicine and biomarker identification as well.Type: GrantFiled: September 4, 2014Date of Patent: June 2, 2015Assignee: Plaxgen Inc.Inventor: Shanmugavel Madasamy
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Patent number: 9040308Abstract: Management of the health status of an animal colony using a plurality of blood collection cards and the analysis of dried blood from members of the colony that has been collected on the cards. Members of the colony may be removed from the colony as a result of the analysis.Type: GrantFiled: February 2, 2015Date of Patent: May 26, 2015Assignee: IDEXX Laboratories, Inc.Inventor: Matthew Howard Myles
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Patent number: 9040304Abstract: A multi-channel system and methods for sorting particles according to one or more characteristics of the particles. The system includes multiple flow cytometry units, each unit can have a nozzle for producing a fluid stream containing a desired population of particles in a mixture of particles. Each of the units may be operable to sort said desired population of particles by interrogating the fluid stream with a beam of electromagnetic radiation and classifying particles based on one or more characteristics of the particles. The system also includes a common fluid delivery system for delivering sheath fluid to each flow cytometer unit for producing respective fluid streams.Type: GrantFiled: March 12, 2014Date of Patent: May 26, 2015Assignee: Inguran, LLCInventors: Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
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Publication number: 20150132245Abstract: The present invention comprises a method for detoxification or measurement of the concentration of at least one compound in a host body, wherein said host body comprises a sorption material of at least one phase over a certain period of time, where sorption of said at least one compound in at least one phase of said sorption material is effected and the content of the at least one compound in said sorption material is optionally analysed. Use of a sorption material for providing an estimate of the concentration of compounds present in a host body, use of a sorption material for detoxification and sorption material for diagnostic use is also comprised in the present application.Type: ApplicationFiled: April 25, 2013Publication date: May 14, 2015Inventors: Kevin V. Thomas, Katherine Hailey Langford, Alfhild Kringstad, Ian John Allan
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Patent number: 9029161Abstract: A method and apparatus for dating a body sample, such as blood, includes taking at least one spectroscopic measurement of the sample at at least two predetermined positions in the spectrum having spectral characteristics corresponding to at least two predetermined substances present in the sample that have a time varying relationship with each other. A measured relative concentration of each of the predetermined substances is then determined from the measurement, and the measured relative concentrations of the two predetermined substances is compared with a known variation of the relative concentrations of the two predetermined substances over time. A good fit of the measured relative concentrations to the known variation of the relative concentrations is then determined, so as to provide an indication of the age of the sample. Alternatively, instead of measuring the relative concentrations of each of the predetermined substances, the rate of change of the relative concentrations is determined.Type: GrantFiled: July 11, 2011Date of Patent: May 12, 2015Assignee: Academisch Medisch CentrumInventors: Maurice Aalders, Rolf Bremmer
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Patent number: 9029165Abstract: A method for detecting electromagnetic waves derived from bacterial DNA, comprising extracting and purifying nucleic acids from a sample; diluting the extracted purified nucleic acids in an aqueous solvent; measuring a low frequency electromagnetic emission over time from the diluted extracted purified nucleic acids in an aqueous solvent; performing a signal analysis of the low frequency electromagnetic emission over time; and producing an output, based on the signal analysis, in dependence on the DNA in the sample. The DNA may be extracted from at least one of blood, feces, urine, saliva, tears, seminal fluid, sweat, seminal and vaginal fluids of a patient, or water to determine, e.g., potability. The samples may be frozen. The extracting and purifying may comprise diluting the sample with an aqueous buffer and mixing; degrading proteins in the diluted sample; precipitating DNA from the buffer solution; and resuspending the precipitated DNA in an aqueous solution.Type: GrantFiled: May 9, 2014Date of Patent: May 12, 2015Inventor: Luc Montagnier
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Patent number: 9029162Abstract: Disclosed are methods and systems for the analysis of testosterone in a sample using supported liquid extraction and liquid chromatography-mass spectrometry.Type: GrantFiled: February 7, 2012Date of Patent: May 12, 2015Assignee: Laboratory Corporation of America HoldingsInventors: Russell Philip Grant, Matthew Crawford, Donald Walt Chandler, William Curtin
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Patent number: 9023281Abstract: Chemical indicator apparatuses containing one or more chemical indicators for use in monitoring the quality of water in an aquatic environment. The apparatuses are designed and configured to be submersible in the water that is being monitored. In some embodiments, each apparatus includes a plurality of immobilized-dye-based chemical indicators that undergo a physical change as levels of one or more constituents of the water change. Such indicators can be read by one or more suitable optical readers. These and other embodiments are designed and configured to be movable by a corresponding monitoring/measuring apparatus, for example, via a magnetically coupled drive. Also disclosed are a variety of features that can be used to provide a chemical indicator apparatus with additional functionalities.Type: GrantFiled: December 13, 2012Date of Patent: May 5, 2015Assignee: Step Ahead Innovations, Inc.Inventor: James E. Clark
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Patent number: 9023283Abstract: A separate excitation and high sensitive resonant type mass sensor is provided. The resonant type mass sensor 1 includes: an oscillator 3; an vibrator 2 placed on the oscillator 3; and a detecting unit 5 for detecting the resonant frequency of the vibrator 2, and is characterized in that the vibrator 2 and the oscillator 3 are not coupled mechanically and that the vibrator 2 is not mechanically coupled to any members. The vibration of the vibrator 2 is represented by a standing wave. The vibrator 2 includes a molecular recognition means for recognizing the molecules of a substance to be measured. The molecular recognition means may collect specific molecules by antigen-antibody reaction. The vibrator 2 may include at least a magnetizable part. To the magnetizable part, magnetic beads 26, to which an antibody or antigen is immobilized, may be adsorbed magnetically.Type: GrantFiled: November 15, 2013Date of Patent: May 5, 2015Assignee: Incorporated National University Iwate UniversityInventor: Masaki Yamaguchi
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Patent number: 9012228Abstract: Provided is a method for checking a blood status including: a step of supplying blood to the centrifugal container of a disk; a step of rotating the disk to centrifuge the blood cells and blood plasma in the centrifuge container, and detecting the actual moving distance per hour of the blood cells in the centrifugal container; and a step of establishing a first graph which represents the actual moving distance of the blood cells per hour, and a second graph which represents the theoretical moving distance of the blood cells per hour, and thereafter calculating the hematocrit of the blood cells and the viscosity of the blood plasma by comparing the first graph with the second graph.Type: GrantFiled: December 12, 2012Date of Patent: April 21, 2015Assignee: Postech Academy-Industry FoundationInventors: Dong Sung Kim, Moonwoo La, Sangmin Park
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Patent number: 9005982Abstract: The present disclosure is generally related to pulmonary autoantigens. The disclosure provides methods and kits for assessing whether a subject has or is predisposed to interstitial lung disease. Additionally the present disclosure provides methods of treatment and animal models of interstitial lung disease.Type: GrantFiled: June 10, 2011Date of Patent: April 14, 2015Assignee: The Regents of the University of CaliforniaInventors: Mark S. Anderson, Anthony K. Shum
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Patent number: 9005981Abstract: The present invention is in the field of identification and characterization of novel insecticidal target sites and, in particular, relates to host cells, assays and antibodies thereto.Type: GrantFiled: December 22, 2009Date of Patent: April 14, 2015Assignee: Dow AgroSciences LLCInventors: Nailah Orr, Gerald B. Watson, Gary D. Gustafson, James M. Hasler, Chaoxian Geng, Scott Chouinard, Kevin R. Cook, Vincent L. Salgado
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Patent number: 8999635Abstract: The present invention relates to the identification of loss-of-function mutations in the filaggrin gene and their use in diagnosing ichthyosis vulgaris and/or susceptibility to other diseases including atopic dermatitis (eczema), asthma, psoriasis and allergies (including food allergy).Type: GrantFiled: December 15, 2006Date of Patent: April 7, 2015Assignee: University Court of The University of DundeeInventors: William Henry Irwin McLean, Frances Jane Dorothy Smith
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Patent number: 8999652Abstract: Mixtures of cell types can be analyzed by having at least two signal markers, with at least one at three different levels to provide a barcode for each cell type. The mixture of cells may be subjected to a common candidate moiety and the effect of the moiety on the cells determined along with identification of the cell by the barcode. Conveniently, surface marker proteins and labeled antibodies can be used to create the barcode and the cells analyzed with flow cytometry.Type: GrantFiled: August 5, 2008Date of Patent: April 7, 2015Assignee: Primity Bio, Inc.Inventors: Peter Oliver Krutzik, Thomas Scott Wehrman
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Patent number: 8999653Abstract: The instant invention provides for methods for detecting the internalization of a transmembrane protein of interest expressed at the surface of a cell. More specifically, the methods involve (a) labelling the protein of interest with a fluorescent metal complex, the lifetime of which is greater than 0.1 ms, (b) adding to the reaction medium a composition capable of causing the internalization of the protein of interest, (c) adding to the reaction medium (1) a modulating agent which is a fluorescent FRET acceptor compound compatible with the fluorescent metal complex, the final concentration of which in the reaction medium is greater than 10?7M; or (2) a reducing agent, the redox potential of which is less than +0.1 V and preferably between 0.25 and 0.Type: GrantFiled: July 30, 2009Date of Patent: April 7, 2015Assignee: Cis-Bio InternationalInventors: Jurriaan Zwier, Robert Poole, Herve Ansanay, Michel Fink, Eric Trinquet
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Patent number: 8999243Abstract: A blood coagulation analysis device and method in which information relating to the coagulability of blood is evaluated based on a change generated in a permittivity measured in a coagulation process of the blood due to addition of a substance that activates or inactivates platelets to the blood.Type: GrantFiled: March 7, 2014Date of Patent: April 7, 2015Assignee: Sony CorporationInventors: Yoshihito Hayashi, MarcAurele Brun, Shinji Omori, Yoichi Katsumoto, Kazumasa Sato
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Patent number: 8999721Abstract: Provided is a method for assessing cardiovascular risk in Metabolic Syndrome and Type 2 Diabetes patients. The method involves obtaining data from a Type 2 diabetes patient or a Metabolic Syndrome patient and determining a Fayad/Schentag index which includes a Glucose Supply Index (S) to an Insulin Demand Index (D) ratio. The Fayad/Schentag index is used in scoring cardiovascular risks of Metabolic Syndrome patients and for recommending and implementing therapeutic interventions that can be shown to lower cardiovascular risk.Type: GrantFiled: January 30, 2013Date of Patent: April 7, 2015Assignee: Therabrake, Inc.Inventors: Jerome J. Schentag, Joseph M. Fayad, Scott V. Monte
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Patent number: 8992835Abstract: Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.Type: GrantFiled: May 2, 2014Date of Patent: March 31, 2015Assignee: Becton, Dickinson and CompanyInventors: Gerrit Jan van den Engh, Timothy Wayne Petersen