Hetero-n Patents (Class 436/96)
  • Patent number: 7358093
    Abstract: The present invention relates to the L-lysine salt of aztreonam and methods for making the L-lysine salt of aztreonam.
    Type: Grant
    Filed: March 22, 2007
    Date of Patent: April 15, 2008
    Assignee: Corus Pharma, Inc.
    Inventors: Viktor Gyollai, Csaba Szabo, Claude Singer, Istvan Bodi
  • Patent number: 7332345
    Abstract: A chemical sensing apparatus and method for the detection of sub parts-per-trillion concentrations of molecules in a sample by optimizing electron utilization in the formation of negative ions is provided. A variety of media may be sampled including air, seawater, dry sediment, or undersea sediment. An electrostatic mirror is used to reduce the kinetic energy of an electron beam to zero or near-zero kinetic energy.
    Type: Grant
    Filed: November 12, 2002
    Date of Patent: February 19, 2008
    Assignee: California Institute of Technology
    Inventors: Murray R. Darrach, Ara Chutjian
  • Publication number: 20080026369
    Abstract: The invention relates to a method for determining the reactive state of a chemical reaction process in a reaction mixture (110), in particular an amplification reaction for nucleic acids. Said method comprises a viscosity determination, which preferably uses a dynamic viscometer (1). The invention also relates to an improved dynamic viscometer (1) for carrying out said method. Said viscometer is characterised by an appropriate choice of material for the resonator (101) and optimised geometric ratios.
    Type: Application
    Filed: November 5, 2004
    Publication date: January 31, 2008
    Inventors: Jürg Dual, Lukas Bestmann, Klaus Häusler
  • Patent number: 7297940
    Abstract: Apparatus, methods, and program products are disclosed that classify spectral peaks in dissociation spectrum data and thereby improves the efficiency of molecular sequencing.
    Type: Grant
    Filed: May 3, 2005
    Date of Patent: November 20, 2007
    Assignee: Palo Alto Research Center Incorporated
    Inventor: Marshall W. Bern
  • Publication number: 20070231313
    Abstract: Methods and systems for detecting azetidine-2-carboxylic acid (Aze) in food consumable by humans and animals are provided. Also provided are methods and systems for inactivating Aze in food and byproducts, as well as other methods for the diagnosis, prevention, and treatment of disorders associated with Aze.
    Type: Application
    Filed: March 29, 2007
    Publication date: October 4, 2007
    Applicant: STANFORD UNIVERSITY
    Inventor: Edward Rubenstein
  • Patent number: 7267994
    Abstract: The present invention provides compositions and methods for detecting, analyzing, and identifying biomolecules. More particularly, the invention provides Element Coded Affinity Tags comprising a metal chelate and a metal ion and methods of using the tags to detect, analyze, and identify biomolecules including polypeptides, nucleic acids, lipids, and polysaccharides.
    Type: Grant
    Filed: May 24, 2004
    Date of Patent: September 11, 2007
    Assignee: Regents of the University of California
    Inventors: Claude F. Meares, Carlito B. Lebrilla, Nathaniel G. Butlin, Sarah M. Cheal, Todd M. Corneillie, Susan Lee, Paul A. Whetstone, Nicolas L. Young
  • Patent number: 7267990
    Abstract: Porphyrins containing one or more neutral or negatively-charged, closo- or nido-carborane substituents are useful as chelators. The carbon-carbon bonds linking the boron-containing groups to the porphyrin ring make the compounds highly resistant to hydrolysis. These compounds have potential for use in selective binding to specific ligands. These compounds are highly stable, soluble in water and organic solvents, and have low toxicity.
    Type: Grant
    Filed: November 12, 2003
    Date of Patent: September 11, 2007
    Assignee: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventor: Maria da Graça Henriques Vicente
  • Patent number: 7264777
    Abstract: A method of quantitatively determining surface area of a sample uses polyvinylpyrrolidone (PVP) dispersed in a solution containing the sample, and determining the amount of PVP that has deposited on the sample.
    Type: Grant
    Filed: August 14, 2003
    Date of Patent: September 4, 2007
    Assignee: The United States of America as represented by the Secretary of the Interior
    Inventors: Alex E. Blum, Dennis D. Eberl
  • Patent number: 7262060
    Abstract: A method for assessing nicotine dependence, which involves measuring a whole blood, serum or platelet-rich plasma serotonin level and rating the whole blood, serum or platelet-rich plasma serotonin level on such a scale that the higher it is, the higher nicotine dependence is.
    Type: Grant
    Filed: October 26, 2004
    Date of Patent: August 28, 2007
    Assignee: Tosoh Corporation
    Inventors: Yuji Hirowatari, Katsuko Hara, Hakuo Takahashi
  • Patent number: 7262293
    Abstract: The present invention relates to the L-lysine salt of aztreonam and methods for making the L-lysine salt of aztreonam.
    Type: Grant
    Filed: July 1, 2004
    Date of Patent: August 28, 2007
    Assignee: Corus Pharma
    Inventors: Viktor Gyollai, Csaba Szabo, Claude Singer, Istvan Bodi
  • Patent number: 7255835
    Abstract: Apparatus and method for acquiring an infrared spectrum of a sample having or suspected to have an amide I band, an amide II band, an amide III band, an amide A band, an OH stretching region or a combination thereof. A representative method includes providing a sample; providing an internal reflecting element (IRE) with a functionalized tip; contacting the sample with the IRE to form a sample-IRE interface; directing a beam of infrared (IR) radiation through the IRE under conditions such that the IR radiation interacts with the sample-IRE interface once; recording a reflectance profile over a range of preselected frequencies, whereby an infrared spectrum of the of a sample having or suspected of having an amide I band, an amide II band, an amide III band, an amide A band, an OH stretching region or a combination thereof, disposed in an aqueous solution is acquired.
    Type: Grant
    Filed: September 4, 2002
    Date of Patent: August 14, 2007
    Assignee: North Carolina State University
    Inventors: Stefan Franzen, Simon E. Lappi
  • Patent number: 7214545
    Abstract: The present invention provides compositions and methods for detecting, analyzing, and identifying biomolecules. More particularly, the invention provides Element Coded Affinity Tags comprising a metal chelate and a metal ion and methods of using the tags to detect, analyze, and identify biomolecules including polypeptides, nucleic acids, lipids, and polysaccharides.
    Type: Grant
    Filed: April 28, 2004
    Date of Patent: May 8, 2007
    Assignee: The Regents of the University of California
    Inventors: Claude F. Meares, Paul A. Whetstone, Todd M. Corneillie, Nathaniel G. Butlin
  • Patent number: 7198955
    Abstract: A method is disclosed whereby the concentration of a blood substitute, such as cross-linked hemoglobin, in a serum or plasma specimen is rapidly and accurately identified and quantified. The method further takes the measured concentration of the blood substitute and uses it to correct for its effect, if any, on a measured analyte concentration, e.g., serum/plasma total protein. Further, the method allows for the determination of the concentration of true hemoglobin in the presence of blood substitutes. The method is carried out in respect of samples contained in a primary or secondary labelled tube, or a pipette tip used to dispense serum or plasma in a blood analyzer.
    Type: Grant
    Filed: October 16, 1997
    Date of Patent: April 3, 2007
    Assignee: NIR Diagnostics Inc.
    Inventors: James Samsoondar, Duncan MacIntyre
  • Patent number: 7148069
    Abstract: The present invention provides a method for the analysis of a compound with amino group (e.g., an amino acid or peptide) contained in a sample and convenient manner with a high sensitivity. The compound with amino group in a sample containing the compound with amino group is labeled with a specific carbamate compound such as p-trimethylammonium anilyl-N-hydroxysuccinimidyl carbamate iodide to enhance the selectivity and sensitivity. The present invention is preferably used in conjunction with mass spectrometry such as MS/MS method to facilitate quantitative analysis. The present invention further provides labeling reagents for mass spectrometry.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: December 12, 2006
    Assignee: Ajinomoto Co., Inc.
    Inventors: Hiroshi Miyano, Akihisa Yahashi, Kazutaka Shimbo, Masakazu Nakazawa, Kazuo Hirayama
  • Patent number: 7144971
    Abstract: A polycarbonate generated from the polymerization of a protected hydroxylated bisphenolic compound, a bisphenol, a monophenolic endcapping agent, a bisphenol haloformate, and a curing compound, and thereafter subjecting the obtained polymer to a reaction with an acidic compound. A hydroxylated charge transport compound may also be included in the polycarbonate.
    Type: Grant
    Filed: August 4, 2004
    Date of Patent: December 5, 2006
    Assignee: Xerox Corporation
    Inventors: Timothy P. Bender, H Bruce Goodbrand
  • Patent number: 7090993
    Abstract: This invention relates to a method of assaying pyrrole-containing biological compounds and chemical compositions that can be used in the method. The method involves contacting a biological compound with one of: a) a bound or bindable derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a detectable molecule which forms a complex with the reaction product; or b) a derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a bound binding agent specific to the biological compound in the reaction product; or c) a binding agent specific to the biological compound, followed by exposure to a derivatizing agent which forms a reaction product with the biological compound, and determining the amount of bound biological compound. There is also provided a method of preparing an antigen.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: August 15, 2006
    Assignee: Rowett Research Institute
    Inventors: Jeffrey D. Brady, Simon P. Robins
  • Patent number: 7067267
    Abstract: This invention relates to a method of assaying pyrrole-containing biological compounds and chemical compositions that can be used in the method. The method involves contacting a biological compound with one of: a) a bound or bindable derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a detectable molecule which forms a complex with the reaction product; or b) a derivatizing agent which forms a reaction product with the biological compound, followed by exposure to a bound binding agent specific to the biological compound in the reaction product; or c) a binding agent specific to the biological compound, followed by exposure to a derivatizing agent which forms a reaction product with the biological compound, and determining the amount of bound biological compound. There is also provided a method of preparing an antigen.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: June 27, 2006
    Assignee: Rowett Research Institute
    Inventors: Jeffrey D. Brady, Simon P. Robins
  • Patent number: 6964844
    Abstract: Water soluble hybrid phthalocyanine derivatives useful in competitive and noncompetitive assays immunoassays, nucleic acid and assays are disclosed and claimed having (1) at least one donor subunit with a desired excitation peak; and (2) at least one acceptor subunit with a desired emission peak, wherein said derivative(s) is/are capable of intramolecular energy transfer from said donor subunit to said acceptor subunit. Such derivatives also may contain an electron transfer subunit. Axial ligands may be covalently bound to the metals contained in the water soluble hybrid phthalocyanine derivatives. Ligands, ligand analogues, polypeptides, proteins and nucleic acids can be linked to the axial ligands of the dyes to form dye conjugates useful in immunoassays and nucleic acid assays.
    Type: Grant
    Filed: April 24, 1998
    Date of Patent: November 15, 2005
    Assignee: Biosite, Inc.
    Inventors: Kenneth F. Buechler, Joseph B. Noar, Lema Tadesse
  • Patent number: 6830933
    Abstract: Amlodipine aspartate and amlodipine maleamide are used as reference standards or markers in determining the purity of amlodipine maleate substances and products.
    Type: Grant
    Filed: August 27, 2001
    Date of Patent: December 14, 2004
    Assignee: Synthon BV
    Inventors: Jacobus M Lemmens, Theodorus H. A. Peters, Peter F. A. Bakker, Frantisek Picha
  • Publication number: 20040242887
    Abstract: The invention discloses deuterated N-substituted &agr;,&agr;-diphenyl &agr;-alkoxy acetic acid aminoalkyl esters as well as the physiologically tolerated salts thereof. Furthermore, the invention concerns the use of deuterated N-substituted &agr;,&agr;-diphenyl &agr;-alkoxy acetic acid aminoalkyl esters for the treatment of hypertonic functional states in the region of the urinary bladder as well as for the preparation of pharmaceutical drugs for the treatment of hypertonic functional states in the region of the urinary bladder.
    Type: Application
    Filed: July 22, 2004
    Publication date: December 2, 2004
    Inventors: Rudolf-Giesbert Alken, Johann Roither, Wolf-Dietrich Hubner, Werner Hrachowina, Thomas Stabingis
  • Patent number: 6790668
    Abstract: The present invention is a method for drug level detection by using a simplified and effective deproteinizing step from body fluids, such as plasma, blood, urine, saliva, tear fluid, followed by drug extraction and measurement using an accurate technique, such as a colorimetric assay or a High-Performance Liquid Chromatography method. In a particular embodiment, the invention is directed to a method to quantify rifampicin in order to monitor its levels in body fluids and also to a Kit for rifampicin concentration measurement.
    Type: Grant
    Filed: September 7, 2000
    Date of Patent: September 14, 2004
    Assignee: Fundacao Oswaldo Cruz - Fiocruz
    Inventors: Milton F. Ferreira, Vera L. Luiza, Eduardo W. Barroso, André L. Gemal
  • Publication number: 20040152900
    Abstract: The present invention is directed to the method of isolation of sulfuric acid mono-[3-({1-[2-(4-fluoro-phenyl)-ethyl]-piperidin-4-yl}-hydroxy-methyl)-2-methoxy-phenyl]ester, a metabolite of the 5HT2A antagonist (+)-&agr;-(2,3-dimethoxyphenyl)-1-[2-(4-fluorophenyl)ethyl]-4-piperidinemethanol.
    Type: Application
    Filed: January 20, 2004
    Publication date: August 5, 2004
    Applicant: Aventis Pharmaceuticals Inc.
    Inventors: Ronald Charles Bernotas, Paul Wayne Brown, Gary Thomas Emmons, Chi-Hsin Richard King
  • Patent number: 6713306
    Abstract: A method for detecting the presence of flunitrazepam in a sample. Flunitrazepam contained within the sample is reduced and hydrolyzed by the addition of a hydrolyzing agent and a reducing agent. A visualizing agent is provided to combine with the reduced and hydrolyzed species corresponding to the flunitrazepam to yield a colored species providing a qualitative colorimetric indication of the presence of flunitrazepam within the sample. The invention is a rugged and dependable method suitable for use in the field.
    Type: Grant
    Filed: September 5, 2001
    Date of Patent: March 30, 2004
    Assignee: R. E. Davis Chemical Corporation
    Inventor: Arthur J. Friedman
  • Patent number: 6649598
    Abstract: Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting specially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dichloro-5-(and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
    Type: Grant
    Filed: September 7, 2001
    Date of Patent: November 18, 2003
    Assignee: Applera Corporation
    Inventors: Steven M. Menchen, Linda G. Lee, Charles R. Connell, N. Davis Hershey, Vergine Chakerian, Sam L. Woo, Steven Fung
  • Patent number: 6620624
    Abstract: With rotating and translating a rotation-translation drum installed in a vacuum chamber of a mass spectrometry interface to constitute a mass spectro-meter, a liquid sample incorporating a dissolved substance to be analyzed in its mass is emitted for the drum from a sample supplying nozzle of the mass spectrometry interface, and the dissolved substance is isolated as a spiral filament on the drum. Then, a laser beam is irradiated onto the filament from a laser source via a laser beam inlet situated at the vacuum chamber and thereby, the dissolved substance is ionized without exposing to the air.
    Type: Grant
    Filed: October 6, 2000
    Date of Patent: September 16, 2003
    Assignee: Okazaki National Research Institutes
    Inventor: Nobuyuki Nishi
  • Patent number: 6608178
    Abstract: Antibody specific for a 5-HTOL compound. A glucuronide with 5-hydroxytryptophol (5-HTOL) characterized in that it comprises the structure of the &bgr;-glucuronide between the 5-hydroxy group of 5-HTOL and D-glucopyranosiduronic acid. An immunoassay and a diagnostic method utilizing the ummunoassay wherein a 5-hydroxytryptophol (5-HTOL) compound is determined by the use of an antibody specific for a 5-HTOL compound is used.
    Type: Grant
    Filed: June 7, 1999
    Date of Patent: August 19, 2003
    Assignee: Alco Dia A.B.
    Inventors: Ragnhild Brandt, Olof Beck, Anders Helander, Rose-Marie Jonsson, Eric Unger, Stefan Borg, Eva Akerblom
  • Publication number: 20030108981
    Abstract: Dipicolinic acid (pyridine 2,6 dicarboxylic acid) is a major component of bacterial spores and is unique in that it has only been found in spores. Dipicolinic acid is synthesized during sporulation and is released during germination or upon hydrolysis or heating. Current methods of analysis are based upon ultraviolet or spectroscopic absorption techniques. Though accurate these methods are time consuming and laborious. This invention describes a convenient calorimetric method that utilizes the color complex formed by the interaction of ferrous iron and reducing agents with dipicolinic acid. The presence of dipicolinic acid from spores or toxins produced by vegetative bacterial agents such as ammonia or other volatile amines within a package or container upon which the indicator is mounted will cause an immediate and slowly reversible color change. Primary public use envelopes and personal protection badges or rings worn inside a room with potentially contaminated mail are specifically suggested.
    Type: Application
    Filed: December 10, 2001
    Publication date: June 12, 2003
    Inventor: William L. Robinson
  • Publication number: 20030096422
    Abstract: Methods and apparatus for the solvent extraction of analytes from a sample are provided. A solvent system is used to extract analytes under elevated temperatures and constant regulated pressures under dynamic conditions. The extracted analytes may then be further analyzed.
    Type: Application
    Filed: November 16, 2001
    Publication date: May 22, 2003
    Inventors: Eng Shi Ong, Soo On Woo, Yuk Lin Yong, Siti Norasikin Binte Apandi
  • Patent number: 6558956
    Abstract: Techniques and devices for detecting and analyzing controlled substances and the like are discussed including highly reactive sensor molecules which are coated on a spectroscopic sample surface (4) and which may chemically react with a given analyte to form a covalently bonded adduct with spectral characteristics unique to the new adduct. The techniques provide the basis of a detection system with high sensitivity and high specificity in which the surface can even be washed to remove interfering or nonreactive compounds. The sensor molecules which comprise the coating (8) may have three major components: a central molecular scaffold (“CMS”), a “tether” terminated by a surface attachment group “SAG,” and a reactive functional group “RFG” which may be highly reactive towards certain classes of molecules.
    Type: Grant
    Filed: December 17, 1999
    Date of Patent: May 6, 2003
    Assignee: The University of Wyoming
    Inventors: Keith T. Carron, Robert C. Corcoran
  • Publication number: 20030040118
    Abstract: An apparatus provides for the detection, the determination of the location or the spatial distribution, and/or the quantification of an amount of a chemical species by allowing the chemical species to come into contact with a fluid medium contained in a permeable capillary, transferring the content of the capillary after the contact to a detector, and detecting the chemical species as the content of the capillary is transferred to a detector. The fluid medium can contain a selected reagent that selectively interacts with the chemical species to produce an optically detectable interaction product. The location and amount of the chemical species are determined from a characteristic of the chemical species or its interaction product measured on the content of the capillary and the time at which the characteristic is detected.
    Type: Application
    Filed: July 30, 2001
    Publication date: February 27, 2003
    Applicant: General Electric Company
    Inventors: Radislav Alexandrovich Potyrailo, Timothy Mark Sivavec
  • Publication number: 20030008406
    Abstract: The absolute configuration of a chiral compound is determined by (i) coordinating the chiral compound to a metalloporphyrin having a carbon chain-crosslinked porphyrin dimer structure in which one of the two porphyrin rings has at least one ethyl or substituent bulkier than ethyl at at least one of the second peripheral carbon atoms from the carbon atom at the carbon chain crosslink site, and (ii) analyzing the resultant coordination compound by circular dichroism spectrophotometry to determine the absolute configuration of the asymmetric carbon based on the sign of the Cotton effect. The chiral compound has an asymmetric carbon bonded to a basic group capable of coordinating to the metal of the other porphyrin ring of the metalloporphyrin dimer or an asymmetric carbon atom adjacent to the carbon atom bonded to the basic group.
    Type: Application
    Filed: May 30, 2002
    Publication date: January 9, 2003
    Inventors: Yoshihisa Inoue, Victor Borovkov, Juha Lintuluoto
  • Patent number: 6472222
    Abstract: A simple screening method is provided for determining biological variations among individuals in a smoking cessation program so that a therapy regimen can be adjusted appropriately for each individual. The method includes the steps of having an individual intake a predetermined dose of nicotine, taking a saliva sample at a predetermined time subsequent to intake of the dose of nicotine, and measuring the nicotine and cotinine levels in the sample. Thereafter, a ratio is calculated which includes both the nicotine and cotinine levels as an indicator of the rate of nicotine metabolism of the individual being screened.
    Type: Grant
    Filed: February 16, 1999
    Date of Patent: October 29, 2002
    Assignee: Via Christi Research, Inc.
    Inventor: W. Dale Horst
  • Publication number: 20020137223
    Abstract: Amlodipine aspartate and amlodipine maleamide are used as reference standards or markers in determining the purity of amlodipine maleate substances and products.
    Type: Application
    Filed: August 27, 2001
    Publication date: September 26, 2002
    Inventors: Jacobus M. Lemmens, Theodorus H.A. Peters, Peter F.A. Bakker, Frantisek Picha
  • Patent number: 6417006
    Abstract: An assay method for detecting potential cardiovascular disease (CVD) in a vascularized subject by assessing the concentration of holo-transcobalamin II (holo-TCII) in a sample from the subject where abnormally low levels of holo-TCII are indicative of CVD or susceptibility to CVD.
    Type: Grant
    Filed: October 5, 2000
    Date of Patent: July 9, 2002
    Assignee: Axis Shield ASA
    Inventor: Erling Sundrehagen
  • Publication number: 20020052017
    Abstract: A method for diagnosing vascular endothelial injury, which comprises measuring the serotonin level in a plasma sample.
    Type: Application
    Filed: August 15, 2001
    Publication date: May 2, 2002
    Applicant: TOSOH CORPORATION
    Inventors: Yuji Hirowatari, Hakuo Takahashi, Katsuko Hara
  • Publication number: 20020019407
    Abstract: The invention is based on the discovery that the levels of one or more methylxanthines in urine samples are significantly decreased in children diagnosed with symptoms of autistic disorder, compared to the levels in normal children, and that levels of xanthines in urine are increased in autistic children. Consequently, the presence in the urine of levels of methylxanthines below a certain range and the level of xanthine above a certain range, are diagnostic of autistic disorder. In another aspect, one or more of these methylxanthines can be used to treat individuals exhibiting symptoms of autistic disorder.
    Type: Application
    Filed: April 12, 2001
    Publication date: February 14, 2002
    Inventors: James R. Rusche, Jundong Zhang
  • Patent number: 6333198
    Abstract: A method of testing the purity or stability to degradation of a sample of lamotrigine or a pharmaceutical dosage form comprising lamotrigine comprises assaying the said sample for the presence of a compound selected from 3-amino-6-(2,3-dichlorophenyl)-1,2,4-triazine-5-(4H)-one (compound A) and N-[5-amino-6-(2,3-dichlorophenyl)-1,2,4-triazine-3-yl]-2,3-dichlorobenzamide (compound B). A process for producing compound B, which is novel, is also disclosed.
    Type: Grant
    Filed: March 10, 1999
    Date of Patent: December 25, 2001
    Assignee: Glaxo Wellcome, Inc.
    Inventors: Lorraine Mary Edmeades, Nigel Arthur Griffith-Skinner, Derek Anthony Hill, Graham Thronton Hill, Terence William Packham
  • Publication number: 20010039054
    Abstract: A simple screening method is provided for determining biological variations among individuals in a smoking cessation program so that a therapy regimen can be adjusted appropriately for each individual. The method includes the steps of having an individual intake a predetermined dose of nicotine, taking a saliva sample at a predetermined time subsequent to intake of the dose of nicotine, and measuring the nicotine and cotinine levels in the sample. Thereafter, a ratio is calculated which includes both the nicotine and cotinine levels as an indicator of the rate of nicotine metabolism of the individual being screened.
    Type: Application
    Filed: February 16, 1999
    Publication date: November 8, 2001
    Inventor: W. DALE HORST
  • Patent number: 6296810
    Abstract: The present invention provides a method and apparatus for automated DNA sequencing. The method of the invention includes the steps of: a) using a processive exonuclease to cleave from a single DNA strand the next available single nucleotide on the strand; b) transporting the single nucleotide away from the DNA strand; c) incorporating the single nucleotide in a fluorescence-enhancing matrix; d) irradiating the single nucleotide to cause it to fluoresce; e) detecting the fluorescence; f) identifying the single nucleotide by its fluorescence; and g) repeating steps a) to f) indefinitely (e.g., until the DNA strand is fully cleaved or until a desired length of the DNA is sequenced).
    Type: Grant
    Filed: June 18, 1997
    Date of Patent: October 2, 2001
    Assignee: Praelux Incorporated
    Inventor: Kevin M. Ulmer
  • Patent number: 6265220
    Abstract: A non-immunochemical method is disclosed for determining homocysteine in a sample. A combination of the sample and a first compound that comprises a cis-1,4-dioxo-2-butene moiety or hydrolytically derived precursor thereto is provided in a liquid medium. The combination is subjected to conditions under which homocysteine reacts with the cis-1,4-dioxo-2-butene moiety or hydrolytically derived precursor thereto to form a product, which is then detected without separation of the product from the medium. A second compound such as a mercaptan containing reagent or an antibody for cysteine can optionally be included to facilitate discrimination of the product formed from homocysteine from products formed from other amines in the sample. The product from the reaction of homocysteine has a six-member ring fused to an unsaturated five-member ring. The junction of the rings is comprised of the nitrogen of homocysteine and an atom that comprises a double bond.
    Type: Grant
    Filed: June 25, 1999
    Date of Patent: July 24, 2001
    Inventor: Edwin F. Ullman
  • Patent number: 6133040
    Abstract: Test kit and method for discriminating cocaine and its salts from substances that falsely give a positive color reaction with Scott's thiocyanate reagent. As all Scott false positive substances have lower pKa values than cocaine, or in their basic from higher pKa values than the cocaine base, they can be discriminated from cocaine by a suitable pH test of the solution. The pH indicator is preferably organic or lipophilic so that it can be dissolved in a solvent immiscible with water. The partitioning of the organic cocaine base or its salt between an organic and water phase of specific pH is used to further distinguish the test substance.
    Type: Grant
    Filed: August 28, 1997
    Date of Patent: October 17, 2000
    Assignee: Identa, Ltd.
    Inventor: Baruch Glattstein
  • Patent number: 6130044
    Abstract: A highly specific surface for biological reactions, which includes a support with at least one essentially compact layer of an organic compound. On the outside of the organic compound layer is an exposed group containing an ethylenic double bond, which has affinity for a molecule with biological activity under certain reaction conditions. The other elements of the organic compound layer are essentially inaccessible to the molecule with biological activity under the reaction conditions.
    Type: Grant
    Filed: May 26, 1998
    Date of Patent: October 10, 2000
    Assignee: Institut Pasteur and Centre National de la Recherche Scientifique
    Inventors: David Bensimon, Aaron Bensimon, Fran.cedilla.ois Heslot
  • Patent number: 6096723
    Abstract: Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5', 7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
    Type: Grant
    Filed: March 23, 1999
    Date of Patent: August 1, 2000
    Assignee: The Perkin-Elmer Corporation
    Inventors: Steven M. Menchen, Linda G. Lee, Charles R. Connell, N. Davis Hershey, Vergine Chakerian, Sam L. Woo, Steven Fung
  • Patent number: 6057161
    Abstract: A high purity styrene-divinyl benzene resin is used for rapid solid phase extraction of drugs of abuse from body fluid samples. Simple extraction procedures are described which provide high recovery and selectivity for a wide variety of drugs. A convenient method of increasing flow rate in such solid phase extractions is also described.
    Type: Grant
    Filed: October 27, 1997
    Date of Patent: May 2, 2000
    Assignee: Varian, Inc.
    Inventor: David R. Nau
  • Patent number: 6054322
    Abstract: A method for the determination of an individual's alcohol consumption, characterized in comprising the steps: i) determining the level of sialic acid in a body fluid sample from the individual; ii) comparing the level found in step (i) with the level for the normal population, an increased level being an indication of an increased alcohol consumption relative to the consumption (per individual) of the normal population.
    Type: Grant
    Filed: February 28, 1997
    Date of Patent: April 25, 2000
    Assignee: Axis-Shield ASA
    Inventors: Pekka Sillanaukee, Kaija Seppa, Ola M.ang.rtensson
  • Patent number: 6034267
    Abstract: The invention provides a pharmaceutical compositions comprising a compound of formula I:R.sub.2.sup.2 N--CH.sub.2 COCH.sub.2 --CH.sub.2 CO--OR.sup.1(I)wherein, R.sup.1 and R.sup.2 have any of the values defined in the specification; or a salt thereof; and a pharmaceutically acceptable carrier or excipient. The invention also provides a method for the diagnosis or photochemotherapeutic treatment of disorders or abnormalities of external or internal surfaces of the body using such compounds or salts.
    Type: Grant
    Filed: December 5, 1997
    Date of Patent: March 7, 2000
    Assignee: PhotoCure AS
    Inventors: Karl E. Gierskcky, Johan Moan, Qian Peng, Harald Steen, Trond Warloe, Alf Bjorseth
  • Patent number: 6004536
    Abstract: The present invention relates to a family of cyanine dyes possessing lipophilic alkyl chains and either one or more reactive functional groups, bromo or chloro, or phenyl, sulfophenyl or polysulfophenyl substituents or combinations thereof. The dyes of the invention are useful for staining membranes in cells or isolated from cells, and are well-retained therein. Additionally, the reactive dyes of the invention are useful for preparing dye-conjugates, thereby conferring the membrane staining ability of the subject dye onto the resulting dye-conjugate.
    Type: Grant
    Filed: August 14, 1996
    Date of Patent: December 21, 1999
    Assignee: Molecular Probes, Inc.
    Inventors: Wai-Yee Leung, Richard P. Haugland, Fei Mao
  • Patent number: 5916815
    Abstract: An assaying system for determining the presence of particular illicit substances in human physiological fluid, consisting of a collection cup, a collection cup lid, and a test card with assaying means, quality control means and adulteration detection means which is secured to the collection cup lid. The assaying means contemplate the addition of control positives such that a pre-determined number of assaying systems would be configured so that a false positive indication would be given by the assaying means in addition to any true positive results which may be present. Accordingly, it would initially be impossible to distinguish true test-positives from control positives, and thus the anonymity of a true test-positive donor individual would be protected. All donor individuals testing negative would be immediately discernible.
    Type: Grant
    Filed: February 14, 1997
    Date of Patent: June 29, 1999
    Assignee: National Medical Review Office Inc.
    Inventor: Murray Lappe
  • Patent number: 5885778
    Abstract: Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5',7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
    Type: Grant
    Filed: August 4, 1997
    Date of Patent: March 23, 1999
    Assignee: The Perkin-Elmer Corporation
    Inventors: Steven M. Menchen, Linda G. Lee, Charles R. Connell, N. Davis Hershey, Vergine Chakerian, Sam L. Woo, Steven Fung
  • Patent number: 5858653
    Abstract: Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes groups capable of attracting the target molecule as well as groups capable of covalently binding to the target molecule, once attracted. Optionally, the composition can contain photoreactive groups for use in attaching the reagent composition to the surface.
    Type: Grant
    Filed: September 30, 1997
    Date of Patent: January 12, 1999
    Assignee: SurModics, Inc.
    Inventors: Lise W. Duran, Melvin J. Swanson, Richard A. Amos, Sheau-Ping J. Hu, Patrick E. Guire