By Measuring A Physical Property (e.g., Mass, Etc.) Patents (Class 506/12)
  • Publication number: 20150005192
    Abstract: The invention relates to a method of diagnosing or monitoring schizophrenia or other psychotic disorder.
    Type: Application
    Filed: September 12, 2014
    Publication date: January 1, 2015
    Applicant: Cambridge Enterprise Limited
    Inventors: Sabine Bahn, Paul Guest, Laura Harris, Yishai Levin, Divya Krishnamurthy
  • Publication number: 20150005194
    Abstract: Processes and methods for creating a database of genomic samples from healthy human donors, methods that use the database to identify and correlate polymorphic genetic markers and other markers with diseases and conditions are provided.
    Type: Application
    Filed: May 9, 2014
    Publication date: January 1, 2015
    Applicant: Sequenom, Inc.
    Inventors: Andreas BRAUN, Hubert Köster, Dirk Van Den Boom, Ping Yip, Charlie Rodi, Liyan He, Norman Chiu, Christian Jurinke
  • Publication number: 20150005195
    Abstract: Biomarkers relating to insulin resistance, pre-diabetes, type-2 diabetes, metabolic syndrome, atherosclerosis, and cardiomyopathy are provided, as well as methods for using such biomarkers as biomarkers for insulin resistance, pre-diabetes, type-2 diabetes, metabolic syndrome, atherosclerosis, and cardiomyopathy. In addition, methods for modulating the respective disorders or conditions of a subject are also provided. Also provided are suites of small molecule entities as biomarkers for insulin resistance, pre-diabetes, type-2 diabetes, metabolic syndrome, atherosclerosis, and cardiomyopathy.
    Type: Application
    Filed: July 7, 2014
    Publication date: January 1, 2015
    Inventors: Yun Fu HU, Costel Chirila, Danny Alexander, Michael Milburn, Matthew W. Mitchell, Walter Gall, Kay A. Lawton
  • Publication number: 20140378325
    Abstract: Methods are provided that detect cognitive impairment including mild cognitive impairment and Alzheimer disease by using a protein or its partial peptide that differs in presence or absence. Novel biomarkers are also provided for cognitive impairment and non-psychiatric disease, as well as methods for detecting cognitive impairment using such biomarkers. Specifically, a biomarker for diagnosis is provided that comprises a protein fragment or peptide of not less than 5 amino acid residues arising from at least one protein or peptide selected from the group of proteins consisting of an amino acid sequence expressed by SEQ ID NO: 1, 3, 6, 8, 10, 13, 15, 18, or 20 and selected from the group of partial peptide in these proteins consisting of an amino acid sequence expressed by SEQ ID NO: 2, 4, 5, 7, 9, 11, 12, 14, 16, 17, 19, or 21.
    Type: Application
    Filed: April 28, 2014
    Publication date: December 25, 2014
    Applicant: MCBI INC.
    Inventors: Kazuhiko UCHIDA, Takashi ISHI, Kohji MENO, Hideaki SUZUKI
  • Publication number: 20140364337
    Abstract: A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.
    Type: Application
    Filed: June 6, 2014
    Publication date: December 11, 2014
    Inventors: Greg Hermanson, John Charles Rogers, Joel Louette, Ryan Daniel Bomgarden, Bhavinkumar Patel
  • Patent number: 8906673
    Abstract: An apparatus and method for counting nanoparticle probes is disclosed. In one embodiment, quantum dot-tagged proteins on optically transparent membranes or slides are counted. The transparent membranes or slides are loaded onto a stage (e.g., an X-Y stage or X-Y-Z stage), which can automatically reposition the transparent membrane or slides for image capture at varying locations. A microscope can be used for providing a light source to fluoresce the nanocrystals and for providing the magnification needed for image capture. Once one or more images are captured, the nanoparticles can be automatically counted using post-processing software that maintains a total count across multiple images, if desired.
    Type: Grant
    Filed: April 29, 2010
    Date of Patent: December 9, 2014
    Assignee: Oregon Health & Science University
    Inventors: Tania Q. Vu, Brian R. Long, Benjamin K. Scholl
  • Publication number: 20140357526
    Abstract: A method for analyzing the polypeptide content of animal tissue is described. The method includes the steps of (a) providing an animal tissue specimen; (b) depositing one or more portions of a hydrogel mixture including a protease on spatially discrete portions of the animal tissue specimen; (c) allowing sufficient time to pass for animal tissue under the hydrogel mixture to be form a digested mixture of animal tissue and hydrogel mixture; (d) removing the digested mixture from the animal tissue and extracting the polypeptides from the digested mixture to provide an extract; and (e) analyzing the polypeptide content of the extract by mass spectrometry.
    Type: Application
    Filed: June 2, 2014
    Publication date: December 4, 2014
    Applicant: Vanderbilt University
    Inventors: Richard Caprioli, Glenn A. Harris, Joshua J. Nicklay
  • Publication number: 20140357527
    Abstract: The disclosure provides for methods and apparatuses relating to technology for monitoring chemical and/or biological reactions. Some methods provided herein relate to utilization of NAPPA technology to create large protein arrays suitable for use in combination with various ISFET arrays to enable massive parallel assays of kinase activity and inhibition. Some devices provided herein relate to CMOS chips which utilize the NAPPA array technology to build protein inventories of interest upon an ISFET architecture. Further devices provided herein are capable, inter alia, of processing the arrays created by the combination of NAPPA technology and ISFET architecture.
    Type: Application
    Filed: December 12, 2012
    Publication date: December 4, 2014
    Inventors: Stuart Lindsay, Joshua Labaer
  • Publication number: 20140357525
    Abstract: Disclosed herein are markers for diagnosing Alzheimer's Disease and/or mild cognitive impairment, for predicting risk of developing Alzheimer's Disease and/or mild cognitive impairment, and for monitoring the efficacy of treatment for Alzheimer's Disease and/or mild cognitive impairment. Also disclosed herein are methods of diagnosing Alzheimer's Disease and/or mild cognitive impairment in a subject in need thereof, for predicting risk of developing Alzheimer's Disease and/or mild cognitive impairment in a subject in need thereof, and for monitoring the efficacy of a treatment for Alzheimer's Disease and/or mild cognitive impairment in the subject.
    Type: Application
    Filed: March 26, 2014
    Publication date: December 4, 2014
    Inventors: Rima F. Kaddurah-Daouk, Pudugramam M. Doraiswamy, Hongjie Zhu, Steve Rozen, Michael A. Kling, John Q. Trajonowski, Steven Arnold
  • Publication number: 20140357501
    Abstract: A peptide is cleaved at various bonding sites into oligopeptides or similar fragments by digestion using proteinase K (S3). The obtained fragments are separated according to their kinds by reversed-phase chromatography and fractionated (S4), and each fragment is subjected to mass spectrometry to determine its mass (S6). For each peptide fragment, an amino-acid composition is calculated from the measured mass, and amino-acid sequence candidates are deduced from that composition. The amino-acid sequence candidates of the other peptide fragments are searched for a fragment having an overlapping portion available for combining two peptide fragments to obtain amino-acid sequence candidates of the original peptide (S7). The masses of the amino-acid sequence candidates are compared with a measured mass derived from a result of mass spectrometry of the original peptide to select a correct sequence (S6 and S8).
    Type: Application
    Filed: March 14, 2014
    Publication date: December 4, 2014
    Applicant: SHIMADZU CORPORATION
    Inventors: Akiyasu Yoshizawa, Takayuki Yoshimori, Yuki Ohta
  • Publication number: 20140357511
    Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.
    Type: Application
    Filed: May 28, 2014
    Publication date: December 4, 2014
    Applicant: DeNovo Sciences
    Inventors: Kalyan Handique, Priyadarshini Gogoi, Yi Zhou, Saedeh Sepehri, Christopher Siemer
  • Publication number: 20140349885
    Abstract: A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological matrices. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.
    Type: Application
    Filed: December 6, 2012
    Publication date: November 27, 2014
    Inventors: Subhakar Dey, Subhasish Purkayastha
  • Publication number: 20140349871
    Abstract: Disclosed herein are methods for large-scale, high-throughput identification of protein-protein interactions and the topologies thereof under physiologically relevant conditions. In one aspect, the disclosure provides methods for identifying one or a plurality of interacting peptides within a biological system comprising obtaining a population of proteins cross-linked with a cleavable protein interaction reporter (PIR) cross-linker, cleaving the PIR crosslinker to produce released peptides and cleaved reporter ions, and analyzing the population of released peptides to identify interacting peptides. Also disclosed are methods for identifying candidate drug compounds, as well as methods of data processing and visualization of protein-protein interactions.
    Type: Application
    Filed: May 21, 2014
    Publication date: November 27, 2014
    Inventors: James E. Bruce, Juan Chavez, Chad Weisbrod
  • Publication number: 20140342945
    Abstract: The present invention relates to a forensic method for identifying an organ penetrated by a corpus delicti.
    Type: Application
    Filed: July 25, 2014
    Publication date: November 20, 2014
    Inventors: Sascha DAMMEIER, Heinz-Dieter WEHNER
  • Publication number: 20140336079
    Abstract: Provided in certain embodiments are new methods for forming azido modified nucleic acid conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling nucleic acids with an azide group.
    Type: Application
    Filed: July 30, 2014
    Publication date: November 13, 2014
    Inventors: Brian AGNEW, Maura Ford, Kyle Gee, Kapil Kumar
  • Publication number: 20140336080
    Abstract: The present invention relates to a method of diagnosing an existing or developing acute pneumonia induced by a microorganism by detecting one or more volatile organic compound(s) in a subject's sample and the use of one or more volatile organic compound(s) for the detection of Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumoniae or Haemophilus influenza and optionally opportunistic pathogen Candida albicans.
    Type: Application
    Filed: December 3, 2012
    Publication date: November 13, 2014
    Applicant: MEON Medical Solutions GmbH & Co.KG
    Inventors: Horst Ruether, Anton Amann
  • Publication number: 20140336073
    Abstract: A method for determining the sensitivity and/or resistance of a patient suffering from a cancer disease to Aurora kinase inhibitor therapy, which comprises determining in vitro in the cancer cells or body fluids taken from the patient the expression of at least one gene selected from a particular group and/or determining in vitro in the cancer cells or body fluids taken from the patient the level of at least one protein selected from a particular group.
    Type: Application
    Filed: December 7, 2012
    Publication date: November 13, 2014
    Applicants: PALACKY UNIVERSITY, OLOMOUC, INSTITUTE OF ANIMAL PHYSIOLOGY AND GENETICS ASCR, V.V.I.
    Inventors: Madhusudhan Reddy Kollaredy, Marian Hajduch, Petr Dzubak, Josef Srovnal, Rita Hrabakova, Hana Kovarova
  • Publication number: 20140323322
    Abstract: A chemical sensor comprising: a hydrogel layer, comprising one or more molecular recognition agents and a 2DPC self-assembling array; and a mirror layer. A method for analyzing a sample or bodily fluid, comprising: obtaining a sample or bodily fluid; placing an amount of the sample or bodily fluid onto a chemical sensor, comprising: a hydrogel layer, comprising a molecular recognition agent and a 2DPC self-assembling array; a tethering hydrogel layer; a mirror layer; and a membrane filter layer, allowing the bodily fluid to interact with the hydrogel layer; and allowing ambient or artificial light to pass through the hydrogel layer onto the mirror layer and observing a change in diffraction versus a control.
    Type: Application
    Filed: October 4, 2012
    Publication date: October 30, 2014
    Inventor: Sanford A. Asher
  • Publication number: 20140323352
    Abstract: The present invention relates to the field of diagnostic methods. Specifically, the present invention contemplates a method for diagnosing pancreatic cancer in a subject, a method for identifying whether a subject is in need for a therapy of pancreatic cancer or a method for determining whether a pancreatic cancer therapy is successful. The invention also relates to tools for carrying out the aforementioned methods, such as diagnostic devices.
    Type: Application
    Filed: November 29, 2012
    Publication date: October 30, 2014
    Applicant: METANOMICS HEALTH GMBH
    Inventors: Regina Reszka, Beate Kamlage, Holger Kalthoff, Bodo Schniewind, Julia Mayerle, Markus M. Lerch, Christian Pilarsky, Robert Grützmann
  • Publication number: 20140316001
    Abstract: A composition including a triglyceride containing glycerol which is esterified with three fatty acids, wherein the three fatty acids include at least one fatty acid selected from caprylic acid, capric acid, ?-linolenic acid, linoleic acid, oleic acid, arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid and docosapentaenoic acid. The total amount of caprylic acid, capric acid, a-linolenic acid, linoleic acid, oleic acid, arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid and docosapentaenoic acid is greater than 60%, based on the total weight of the fatty acids in a base or starting emulsion. The various physical mixtures of triglycerides containing the primary therapeutic fatty acid can be blended to form pre-defined amounts for the treatment of various acute pathological conditions.
    Type: Application
    Filed: October 24, 2012
    Publication date: October 23, 2014
    Applicant: STABLE SOLUTIONS LLC
    Inventor: David F. Driscoll
  • Publication number: 20140309142
    Abstract: A method of synthesizing a nucleic acid molecule, such as a gene, on a substrate or microchip is described. In particular, a method for synthesizing, amplifying, and assembling DNA oligonucleotides into a nucleic acid molecule or gene product, on a single substrate or microchip is described. Also described are a method of correcting a sequence error in a synthesized nucleic acid molecule, as well as a method for synthesizing and screening a library of codon variants to identify a nucleic acid molecule with an optimized level of protein expression.
    Type: Application
    Filed: April 16, 2013
    Publication date: October 16, 2014
    Inventor: Jingdong TIAN
  • Publication number: 20140309143
    Abstract: Improved compositions, methods, apparatus, and kits for high-throughput nucleic acid amplification, detection and sequencing are disclosed. A nucleic acid cluster having an identifiable center is produced by generating on a solid support an immobilized nucleic acid complement from a template, one of which comprises a detectable label; and amplifying the complement and the template to obtain a nucleic acid cluster on the support, the cluster having a substantially central location marked by the detectable label and a surrounding region comprising immobilized copies. Also disclosed are nucleotide sequence determination in nucleic acid clusters so produced, center position annotation in the clusters, assignment of sequence information to overlapping clusters, and related compositions and methods.
    Type: Application
    Filed: June 24, 2014
    Publication date: October 16, 2014
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Alan D. Kersey, Jonathan Mark Boutell
  • Patent number: 8853133
    Abstract: This invention pertains to methods, mixtures, kits and compositions pertaining to analyte determination and/or quantification by mass spectrometry using compounds comprising a reporter moiety and a non-encoded detectable label. The compounds can be used in sets for the analysis of mixtures of labeled analytes.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: October 7, 2014
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Subhasish Purkayastha, Subhakar Dey
  • Publication number: 20140296108
    Abstract: The disclosure provides biomarker panels, methods and kits for determining the probability for preeclampsia in a pregnant female. The present disclosure is based, in part, on the discovery that certain proteins and peptides in biological samples obtained from a pregnant female are differentially expressed in pregnant females that have an increased risk of developing in the future or presently suffering from preeclampsia relative to matched controls. The present disclosure is further based, in part, on the unexepected discovery that panels combining one or more of these proteins and peptides can be utilized in methods of determining the probability for preeclampsia in a pregnant female with relatively high sensitivity and specificity. These proteins and peptides disclosed herein serve as biomarkers for classifying test samples, predicting a probability of preeclampsia, monitoring of progress of preeclampsia in a pregnant female, either individually or in a panel of biomarkers.
    Type: Application
    Filed: March 14, 2014
    Publication date: October 2, 2014
    Applicant: SERA PROGNOSTICS, INC.
    Inventors: Durlin Edward HICKOK, John Jay BONIFACE, Gregory Charles CRITCHFIELD, Tracey Cristine FLEISCHER
  • Publication number: 20140287953
    Abstract: A laser ablation cell (1) comprises a flow channel (11) having an essentially constant cross-sectional area so as to ensure a strictly laminar flow in the flow channel. A sample chamber (21) is provided adjacent to a lateral opening (14) of the flow channel. A laser beam (41) enters the sample chamber (21) through a lateral window (16) and impinges on a surface (24) of a sample (23) to ablate material from the sample. The sample may be positioned in such a distance from the flow channel that the laser-generated aerosol mass distribution has its center within the flow channel. This leads to short aerosol washout times. The laser ablation cell is particularly well suited for aerosol generation in inductively coupled plasma mass spectrometry (ICPMS), including imaging applications.
    Type: Application
    Filed: March 24, 2014
    Publication date: September 25, 2014
    Inventors: Detlef Günther, Daniel Grolimund, Hao Wang
  • Publication number: 20140287948
    Abstract: The disclosure provides biomarker panels, methods and kits for determining the probability for preterm birth in a pregnant female. The present disclosure is based, in part, on the discovery that certain proteins and peptides in biological samples obtained from a pregnant female are differentially expressed in pregnant females that have an increased risk of developing in the future or presently suffering from preterm birth relative to matched controls. The present disclosure is further based, in part, on the unexepected discovery that panels combining one or more of these proteins and peptides can be utilized in methods of determining the probability for preterm birth in a pregnant female with relatively high sensitivity and specificity. These proteins and peptides disclosed herein serve as biomarkers for classifying test samples, predicting a probability of preterm birth, monitoring of progress of preterm birth in a pregnant female, either individually or in a panel of biomarkers.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 25, 2014
    Applicant: Sera Prognostics, Inc.
    Inventors: John Jay BONIFACE, Gregory Charles CRITCHFIELD, Durlin Edward HICKOK
  • Publication number: 20140287958
    Abstract: The invention provides a liquid cell for an atomic force microscope. The liquid cell includes a liquid cell housing with an internal cavity to contain a fluid, a plurality of conductive feedthroughs traversing the liquid cell housing between the internal cavity and a dry side of the liquid cell, a cantilevered probe coupled to the liquid cell housing, and a piezoelectric drive element disposed on the cantilevered probe. The cantilevered probe is actuated when a drive voltage is applied to the piezoelectric drive element through at least one of the conductive feedthroughs. A method of imaging an object in a liquid medium and a method of sensing a target species with the liquid cell are also disclosed.
    Type: Application
    Filed: June 9, 2014
    Publication date: September 25, 2014
    Inventors: Jesse D. Adams, Benjamin S. Rogers, Todd A. Sulchek
  • Publication number: 20140287936
    Abstract: The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed.
    Type: Application
    Filed: December 13, 2013
    Publication date: September 25, 2014
    Applicants: CORNELL RESEARCH FOUNDATION, INC., METABOLON, INC.
    Inventors: Rima Kaddurah-Daouk, Bruce S. Kristal
  • Publication number: 20140287947
    Abstract: The disclosure provides biomarker panels, methods and kits for determining the probability for preeclampsia in a pregnant female. The present disclosure is based, in part, on the discovery that certain proteins and peptides in biological samples obtained from a pregnant female are differentially expressed in pregnant females that have an increased risk of developing in the future or presently suffering from preeclampsia relative to matched controls. The present disclosure is further based, in part, on the unexepected discovery that panels combining one or more of these proteins and peptides can be utilized in methods of determining the probability for preeclampsia in a pregnant female with relatively high sensitivity and specificity. These proteins and peptides dislosed herein serve as biomarkers for classifying test samples, predicting a probability of preeclampsia, monitoring of progress of preeclampsia in a pregnant female, either individually or in a panel of biomarkers.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 25, 2014
    Applicant: Sera Prognostics, Inc.
    Inventors: John Jay BONIFACE, Gregory Charles CRITCHFIELD, Durlin Edward HICKOK
  • Publication number: 20140287957
    Abstract: An entire complement or plurality of isotopically labeled amino acids are introduced into the diet of a test subject. Sufficient amounts of the isotopically labeled amino acids are provided to the subject in order to ensure that the subject incorporates a large percentage of isotopically labeled amino acids into newly synthesized proteins. Tissue samples are removed from the subject at different points in time and proteins are extracted and separated so that different proteins of different tissues can be individually analyzed and their amount and pattern of isotopic labeling can be determined. In a preferred embodiment, the methodology can be combined with proteolytic digestion to peptides and analysis by mass spectrometry in order to measure rates of protein turnover in vivo relating to thousands of different proteins.
    Type: Application
    Filed: June 17, 2011
    Publication date: September 25, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Stanley B. Prusiner, John C. Price, Sina Ghaemmaghami, Al Burlingame, Shenheng Guan
  • Patent number: 8841071
    Abstract: The invention generally relates to methods for sample multiplexing. In certain embodiments, methods of the invention obtaining a plurality of different reactant molecules, attaching a unique identifier to the reactant molecules, and forming a droplet including the reactant molecules.
    Type: Grant
    Filed: May 31, 2012
    Date of Patent: September 23, 2014
    Assignee: Raindance Technologies, Inc.
    Inventor: Darren Link
  • Publication number: 20140274737
    Abstract: The invention includes methods and apparatus for isolating and recovering mutations, especially rare and unknown mutations, without amplifying the sample. In particular, using the disclosed methods, it is possible to separate heteroduplexed nucleic acid strand pair from homoduplexed nucleic acid strand pairs having similar sequences and being at a much higher concentration. Once the heteroduplexed nucleic acids are isolated and recovered, it is straightforward to analyze the sequences of the heteroduplexed nucleic acids, e.g., using sequencing or hybrid assays.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Applicant: Boreal Genomics Corp.
    Inventors: Andrea Marziali, Joel Pel
  • Publication number: 20140274799
    Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay of at least three targets. In an exemplary method, partitions may be formed each including a portion of a same sample. The sample may include a first target that is a reference sequence, a second target that is a variant sequence from a gene of interest, and a third target that is a normal sequence from the gene of interest. The target may be amplified in the partitions and amplification data may be collected. A level of the second target may be determined. A copy number of the third target may be determined from a level of the first target and a level of the third target.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Inventors: Ryan T. Koehler, Svilen S. Tzonev
  • Publication number: 20140274783
    Abstract: Isolated, Chlamydia-specific, IL-13 expressing CD8+ T cell clones are provided for understanding the biology of Chlamydia infection and screening of therapeutics. Furthermore, methods are provided for isolating CD8+ T cells comprising the steps of infecting or identifying a naturally infected mammal with at least one species of Chlamydia causing bacteria, allowing the bacteria to clear or for a T cell immune response to the natural infection, collecting immune splenocytes from the mammal, providing at least one antigen from a Chlamydia-specific bacteria, and expanding and depleting the CD4+ T cell population or purifying the CD8 T cell population to isolate IL-13 expressing CD8+ T cell clones.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Inventor: Raymond M. Johnson
  • Patent number: 8835361
    Abstract: The invention provides a high-throughput method for quantitating plant seed proteins, e.g. seed allergens. Such method involves obtaining a protein sample from the seed; analyzing the sample using mass spectrometry with a set of pre-designed internal standard peptides that are specific to the plant allergens; and monitoring frequencies and intensities of resulting spectra to obtain relative and absolute allergen contents in the seed. The invention also provides a system for high-throughput profiling of plant seed allergens. Such system comprises a set of pre-designed internal standard peptides that are specific to the plant seed allergens and one or more mass spectrometers.
    Type: Grant
    Filed: May 31, 2011
    Date of Patent: September 16, 2014
    Assignee: The Curators of the University of Missouri
    Inventors: Jay J. Thelen, Severin E. Stevenson
  • Patent number: 8835362
    Abstract: A mechanism is provided for utilizing a nanodevice to distinguish molecules with different structure. The molecules translocate through or across a nanochannel filled with a electrolyte solution. An electrical signal through the nanochannel is measured for every translocation event. Inner surfaces of the nanochannel include a functional layer, which is a coating to functionalize the nanochannel, in which the functional layer is configured to interact with predetermined ones of the molecules during translocation events. It is determined that a combination of at least two different molecules is formed based on predetermined ones of the molecules interacting with the functional layer to change the electrical signal and/or change a translocation time for the translocation event.
    Type: Grant
    Filed: December 26, 2012
    Date of Patent: September 16, 2014
    Assignees: International Business Machines Corporation, The University of Melbourne, Florey Institute of Neuroscience and Mental Health
    Inventors: Matthew Downtown, Natalie Gunn, Stefan Harrer, Priscilla Rogers, John Wagner, Ross Bathgate, Daniel James Scott, Stan Skafidas
  • Patent number: 8835360
    Abstract: A method for selecting combinations of drugs for treatment of diseases that arise from deranged signaling pathways is disclosed. The method involves measuring the activity states for signaling proteins in a diseased cell and determining whether the activity states are different from the activity states observed for a reference cell such as a normal cell. Based on the observed differences, combinations of two or more drugs are selected to reduce these differences. Treatment of a subject with the combinations restores the activity states of the signaling proteins of the deranged disease-associated signaling pathways toward the activity states observed in the reference cell. Since the diseased cell and the reference cell can both be obtained from the same subject, combinations of drugs that specifically target patient-specific signaling derangements is possible.
    Type: Grant
    Filed: April 24, 2012
    Date of Patent: September 16, 2014
    Assignee: The United States of America as represented by the Secretary of the Department of HHS
    Inventors: Arpita I. Mehta, Lance A. Liotta, Emanuel F. Petricoin
  • Publication number: 20140249055
    Abstract: A system for detecting an array of samples having detectable samples and at least one reference sample is provided. The system comprises an electromagnetic radiation source, a sensing surface comprising a plurality of sample fields, wherein the plurality of sample fields comprise at least one reference field, a phase difference generator configured to introduce differences in pathlengths of one or more samples in the array of samples, and an imaging spectrometer configured to image one or more samples in the array of samples.
    Type: Application
    Filed: September 28, 2012
    Publication date: September 4, 2014
    Inventors: Masako Yamada, Sandip Maity, Sameer Dikar Vartak, Rajesh Langoju, Abhijit Patil
  • Publication number: 20140249049
    Abstract: Biomarkers for pre-Diabetes, Diabetes and/or a Diabetes related conditions, and methods of their use, including the biomarkers in Tables 1 and 2 such as peroxiredoxin-2, complement C1q subcomponent subunit B, sulfhydryl oxidase 1 and apolipoprotein A-IV.
    Type: Application
    Filed: May 14, 2014
    Publication date: September 4, 2014
    Applicants: PROTEOMICS INTERNATIONAL PTY LTD, THE UNIVERSITY OF WESTERN AUSTRALIA
    Inventors: Thomas STOLL, Scott BRINGANS, Kaye WINFIELD, Tammy CASEY, Wendy DAVIS, Kirsten PETERS, Timothy DAVIS, Richard LIPSCOMBE
  • Publication number: 20140235504
    Abstract: A method and a device for analyzing a liquid is provided, in which the liquid is distributed to a plurality of containers, and which enables the rapid and inexpensive performing of a multitude of analyses in liquids, in particular DNA analyses, in micro-arrays. The liquid is supplied to a transparent analytical plate which includes a matrix with a plurality of at least unilaterally closed receptacle chambers arranged adjacent to each other. Axially-displaceable plungers are moved towards walls of the receptacle chambers and the analytical plates are transported towards the plungers using a transport apparatus. The liquid can be distributed into the receptacle chambers using mobile elements of the analytical plate, and the elements may be moved by the plungers. At least one axially displaceable plunger may be heated to a preset temperature value and impart this temperature to the accommodating chamber.
    Type: Application
    Filed: September 25, 2012
    Publication date: August 21, 2014
    Applicant: ASPRE AG
    Inventor: Friedrich-Josef Sacher
  • Publication number: 20140235503
    Abstract: Disclosed is a prediction method of glomerular filtration rate (GFR) from urine samples after kidney transplantation to provide an information needed for predict renal function after the transplantation, more particularly to a prediction method of glomerular filtration rate (GFR) from urine samples after kidney transplantation, which comprises detecting metabolic profiles of five biomarkers, 5a-androst-3-en-17-one (AS), glycocholic acid (GC), sphingosine (SG), tryptophan (TR) and histidine (HT), from urine samples of patients. Glomerular filtration rate (GFR) after kidney transplantation can be predicted more rapidly and precisely to provide an information needed for predict renal function after the transplantation by using five metabolites as biomarkers. The method provides more specific, sensitive, and reliable biomarkers that monitor clinical outcomes and adverse renal events after kidney transplantation, such as rejection, drug toxicity, delayed graft function, and infection.
    Type: Application
    Filed: July 31, 2013
    Publication date: August 21, 2014
    Applicant: KYUNGPOOK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventors: Yong-Lim KIM, Young-Ran YOON, Jung-Ju SEO
  • Publication number: 20140234431
    Abstract: The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. The invention also relates to products, kits, and databases for use in the methods of the invention.
    Type: Application
    Filed: January 28, 2014
    Publication date: August 21, 2014
    Applicant: Massachusetts Institute of Technology
    Inventors: Sangeeta N. Bhatia, Geoffrey A. von Maltzahn, Gabriel A. Kwong
  • Publication number: 20140235473
    Abstract: The present Invention provides a method for diagnosing and/or prognosing Parkinson's disease dementia (PDD) comprising the step of detecting O-glycosylation. in a protein comprising a Ser/Thr motive, in particular Serpin A1, and/or the level of sialic acid on a protein comprising a Ser/Thr motive, in particular Serpin A1. Further, the present invention relates to a molecule for detecting O-linked glycomoieties in a protein comprising a Ser/Thr motive, in particular Serpin A1, and/or glycosylated i so forms of a Ser/Thr motive comprising protein, in particular Serpin A1, for use in the diagnosis and/or prognosis of Parkinson's disease dementia (PDD), Furthermore, the present invention relates to means for diagnosing and/or prognosing Parkinson's disease dementia (PDD) and a kit for diagnosing and/or prognosing Parkinson's disease dementia (PDD).
    Type: Application
    Filed: June 22, 2012
    Publication date: August 21, 2014
    Inventors: Markus Otto, Stefan Lehnert, Sarah Jesse, Olaf Jahn
  • Publication number: 20140228229
    Abstract: The present invention relates generally to methods for identifying drug side effects by detecting perturbations in organ-specific molecular blood fingerprints. The invention further relates to methods for identifying drug-specific organ-specific molecular blood fingerprints. As such, the present invention provides compositions comprising organ-specific proteins, detection reagents for detecting such proteins, and panels and arrays for determining organ-specific molecular blood fingerprints.
    Type: Application
    Filed: December 9, 2013
    Publication date: August 14, 2014
    Applicant: Institute for Systems Biology
    Inventors: Leroy Hood, Biaoyang Lin
  • Publication number: 20140221249
    Abstract: A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.
    Type: Application
    Filed: February 5, 2013
    Publication date: August 7, 2014
    Applicant: GENIA TECHNOLOGIES, INC.
    Inventor: Genia Technologies, Inc.
  • Publication number: 20140221236
    Abstract: Panels, kits, and methods for diagnosing or predicting the likelihood of occurrence of preterm birth (PTB) in a subject are disclosed. An exemplary panel includes at least one of a first marker from a first group of markers and a second marker from a second group of markers. A significant decrease in the first marker and a significant increase in the second marker measured in a sample taken from a subject during the second trimester of pregnancy are diagnostic or predictive of an increased risk of preterm birth for the subject.
    Type: Application
    Filed: February 3, 2014
    Publication date: August 7, 2014
    Inventors: Kristi S. Borowski, Jeff Murray, Kelli K. Ryckman
  • Publication number: 20140213459
    Abstract: The present invention relates to methods for improving the folding stability of antibodies, to antibodies with improved folding stability, nucleic acid and vectors encoding such antibodies, and to uses of such antibodies, nucleic acid and vectors.
    Type: Application
    Filed: May 29, 2012
    Publication date: July 31, 2014
    Inventor: Roland Beckmann
  • Publication number: 20140213467
    Abstract: Compositions and methods which indicate an increased risk for pancreatic carcinoma in a test subject are disclosed.
    Type: Application
    Filed: March 19, 2012
    Publication date: July 31, 2014
    Inventor: Anthony T. Yeung
  • Publication number: 20140213478
    Abstract: Specific peptides, and derived ionization characteristics of the peptides, from the Receptor Tyrosine-Protein Kinase erbB-4 Protein (HER4) protein are provided that are particularly advantageous for quantifying the HER4 protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed where the biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded.
    Type: Application
    Filed: April 4, 2014
    Publication date: July 31, 2014
    Inventors: David B. KRIZMAN, Wei-Li LIAO, Sheeno THYPARAMBIL, Todd HEMBROUGH
  • Patent number: 8791053
    Abstract: The invention relates to a spatially encoded polymer matrix in the form of a bead or a granule for combinatorial solid phase synthesis, assaying, functional proteomics and diagnostic use. Compositions of such beads or granules are also provided. Each beaded polymer matrix of the composition comprises a plurality of spatially immobilized particles. The spatial immobilization of the particles confers on each beaded polymer matrix a “fingerprint” which enables identification of unique beads in a population of beads. The unique identification of individual beads makes it possible to perform combinatorial chemistry strategies while logging individual chemical transformation. Also provided are methods for detection of relative positions in space of particles, methods for generating matrices, methods for distance matrix determination, methods for identifying individual matrices and devices for recording and storing images of matrices.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: July 29, 2014
    Assignee: MPM-Holding APS
    Inventors: Soren Flygenring Christensen, Jens Hog Truelsen, Morten Meldal, Roice Michael, Ib Johannsen