Rna Or Dna Which Encodes Proteins (e.g., Gene Library, Etc.) Patents (Class 506/17)
  • Patent number: 10745696
    Abstract: The present invention provides for a library of vectors comprising human HC-CDR3 regions of varying length, wherein the diversity of said library is focused on the HC-CDR3 region only and diversity has been optimized such that redundancy is reduced for short HC-CDR3 loops and coverage of HC-CDR3 region variants for longer loop lengths has been increased. The library of the present invention is displayed on phage for selection against target antigens.
    Type: Grant
    Filed: February 14, 2019
    Date of Patent: August 18, 2020
    Assignee: ITALFARMACO S.P.A.
    Inventors: Armin Lahm, Christian Steinkuehler, Gessica Filocamo
  • Patent number: 10487358
    Abstract: Aspects of the invention relate to methods for preparing and analyzing a sequencing library from a mixed cell-free DNA (cfDNA) sample, wherein the mixed sample includes double-stranded DNA (dsDNA), damaged dsDNA (e.g., nicked dsDNA), and single-stranded DNA (ssDNA) molecules. The subject methods facilitate the collection of information from dsDNA, ssDNA and damaged DNA (e.g., nicked DNA) molecules in a sample, thereby providing enhanced diagnostic information as compared to sequencing libraries that are prepared from dsDNA alone.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: November 26, 2019
    Assignee: GRAIL, Inc.
    Inventors: Matthew H. Larson, Hyunsung John Kim, Nick Eattock, Xiao Yang
  • Patent number: 10323271
    Abstract: Provided are: a probe reagent which is capable of stably yielding a fluorescence signal in FISH while inhibiting non-specific adsorption by the use of a nucleic acid molecule having a smaller number of bases than a BAC probe; and FISH using the probe reagent. The probe reagent is for in situ hybridization and comprises: phosphor-integrated nanoparticles containing phosphors integrated therein; and a nucleic acid molecule having a prescribed nucleic acid sequence, which phosphor-integrated nanoparticles and nucleic acid molecule are bound with each other.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: June 18, 2019
    Assignee: KONICA MINOLTA, INC.
    Inventors: Takeshi Isoda, Mitsuru Sekiguchi, Yutaka Hatanaka, Yoshikazu Kurihara
  • Patent number: 10266903
    Abstract: Primers and probes specific to the genes encoding extended spectrum beta-lactamase that involves CTX-M groups 1 and 9 that cause extended beta-lactamase resistance in bacteria are described herein, with methods and kits for using these primers and probes to detect CTX-M groups 1 and 9 nucleic acids. In the methods described, nucleic acids present in a clinical or test sample obtained from a biological sample or tissue suspected of containing the CTX-M groups 1 and 9 gene are amplified and corresponding sequences for CTX-M groups 1 and 9 are detected. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (FRET), radiolabels, enzyme labels, and the like.
    Type: Grant
    Filed: December 3, 2015
    Date of Patent: April 23, 2019
    Assignee: ELITECHGROUP, INC.
    Inventors: Irina A. Afonina, Yevgeniy S. Belousov
  • Patent number: 10087261
    Abstract: The invention relates generally to polypeptides, such as antibody molecules, that demonstrate high stability and solubility. In particular, the invention relates to polypeptides comprising paired VL and VH domains that demonstrate soluble expression and folding in a reducing or intracellular environment. The invention also relates to polynucleotides encoding such polypeptides, to libraries of such polypeptides or polynucleotides, and to methods of using such polypeptides in research, diagnostic and therapeutic applications.
    Type: Grant
    Filed: February 14, 2014
    Date of Patent: October 2, 2018
    Assignee: Affinity Biosciences Pty Ltd
    Inventors: Matthew David Beasley, Keith Philip Niven, Ben Ross Kiefel
  • Patent number: 10087484
    Abstract: Provided is a method for stably synthesizing an error-free gene using a high-depth oligonucleotide tiling, which includes designing an oligonucleotide fragment by an over-overlapping method, synthesizing the oligonucleotide fragment using DNA microarray, retrieving error-free oligonucleotide fragments retrieved by next generation sequencing, and assembling the error-free oligonucleotide fragments.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: October 2, 2018
    Assignee: UNIVERSITY-INDUSTRY FOUNDATION, YONSEI UNIVERSITY
    Inventors: Duhee Bang, Nam Jin Cho, Han Na Seo, Eui Jin Kwon
  • Patent number: 9988670
    Abstract: Primers and probes specific to genes encoding carbapenem-resistant Enterobacteriaceae (CREs) that include KPC (Klebsiella pneumoniae carbapenemase), NDM-1 (New Delhi Metallo-beta-lactamase), VIM (Verona Integron-Mediated Metallo-?-lactamase), IMP-type carbapenemase and OXA 48 (oxacillinase), that cause resistance in Enterobacteriaceae bacteria, are described herein, with methods and kits for using these primers and probes to detect target nucleic acids. In the methods described, nucleic acids present in a clinical or test sample obtained from a biological sample or tissue suspected of containing the the NDM1, KPC, IMP, VIM and OXA genes are amplified and corresponding sequences for the NDM1, KPC, IMP, VIM and OXA genes are detected. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (FRET), radiolabels, enzyme labels, and the like.
    Type: Grant
    Filed: December 10, 2015
    Date of Patent: June 5, 2018
    Assignee: ELITECHGROUP B.V.
    Inventors: Irina A. Afonina, Yevgeniy S. Belousov
  • Patent number: 9890190
    Abstract: Methods of conjugating oligonucleotides are provided. The methods may include: activating a terminal of first oligonucleotide using a squarate reagent to produce an activated first oligonucleotide; and binding the first oligonucleotide and a second oligonucleotide to a splint oligonucleotide; to conjugate the activated first oligonucleotide with a terminal of the second oligonucleotide via a squaramide linkage to produce a squaramide-linked oligonucleotide. Also provided are oligonucleotides that include a squaramide internucleoside linkage. Compositions are provided that include a first oligonucleotide including 300 or more nucleosides and at least one squaramide internucleoside linkage; and a second complementary oligonucleotide not including a squaramide linkage. Kits and compositions for practicing the subject methods are also provided.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: February 13, 2018
    Assignee: Agilent Technologies, Inc.
    Inventors: Kenneth W. Hill, Douglas J. Dellinger, Benjamin D. Lunstad
  • Patent number: 9845511
    Abstract: The invention described herein provides for methods and systems for determining, selecting, and/or treating diseases and conditions caused by or associated with high quantities of methanogens in a subject, or diseases and conditions caused by or associated with low quantities of methanogens in a subject. In various embodiments, a therapy to inhibit the growth of methanogens or to promote the growth of methanogens are selected and/or administered to a subject in need thereof.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: December 19, 2017
    Assignee: CEDARS-SINAI MEDICAL CENTER
    Inventors: Mark Pimentel, Ruchi Mathur, Christopher Chang
  • Patent number: 9844764
    Abstract: The present invention relates to a protein or peptide printing method, comprising (a) a step for preparing nucleic acids and a cell-free protein synthesis system in an engraved plate composed of microscopic grooves having a specific opening shape, (b) a step for superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and (c) a step for synthesizing the protein or peptide from the nucleic acids using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate along the specific opening shapes of the microscopic grooves.
    Type: Grant
    Filed: February 21, 2013
    Date of Patent: December 19, 2017
    Assignees: University of Tokyo, Nikon Corporation
    Inventors: Takanori Ichiki, Manish Biyani, Hirofumi Shiono
  • Patent number: 9605237
    Abstract: The present invention relates to yeast strains and, in particular, to yeast strains for use in fermentation processes. The invention also relates to methods of fermentation using the yeast strains of the invention either alone or in combination with other yeast strains. The invention further relates to methods for the selection of yeast strains suitable for fermentation cultures by screening for various metabolic products and the use of specific nutrient sources.
    Type: Grant
    Filed: March 6, 2009
    Date of Patent: March 28, 2017
    Assignee: Auckland Uniservices Limited
    Inventors: Matthew Robert Goddard, Richard Clague Gardner, Nicole Anfang
  • Patent number: 9453218
    Abstract: The invention relates to a method for producing a DNA sequence for a nucleic acid library having a plurality of elements. The synthesized DNA sequence comprises at least three sections. The first section and the third section in each element have defined sequences (D1, D2) that are identical in each element of the nucleic acid library. In a middle second section, at least two adjoining variable codon triplets are arranged.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: September 27, 2016
    Inventor: Markus Fuhrmann
  • Patent number: 9334492
    Abstract: A protein-immobilizing solid phase is a protein-immobilizing solid phase comprising an mRNA-nucleic acid linker-protein complex, obtained by linking the mRNA and the protein encoded by that mRNA through the nucleic acid linker, immobilized on the solid phase, wherein the nucleic acid linker has a photocleavage site and a solid phase binding site.
    Type: Grant
    Filed: April 30, 2014
    Date of Patent: May 10, 2016
    Assignees: The University of Tokyo, NIKON CORPORATION
    Inventors: Shingo Ueno, Naoto Nemoto, Takanori Ichiki, Hirofumi Shiono, Hisao Osawa
  • Patent number: 9296771
    Abstract: A method of attaching a phosphorous dendrimer onto magnetic microparticles by taking magnetic microparticles in a water-based solution, then performing a solvent exchange, then suspending the microparticles in a phosphorous dendrimer solution and shaking, then washing the microparticles with an organic solvent, and then washing the microparticles with a transition solvent. The solvent exchange is done by washing the microparticles with a first concentration of a transition solvent, then washing the microparticles with a second concentration of the transition solvent where the second concentration is greater than the first concentration, then washing the microparticles with an organic solvent, then washing the microparticles with the transition solvent, then washing the microparticles with the organic solvent, and then suspending the microparticles in the transition solvent. Also disclosed is the related phosphorous dendrimer made by this method.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: March 29, 2016
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Marie J. Archer, Baochuan Lin
  • Publication number: 20150133317
    Abstract: Disclosed herein are compositions and methods for sequencing, analyzing, and utilizing samples such as single samples. Also disclosed herein are compositions and methods for matching together two or more sequences from a sample. Also disclosed herein are compositions and methods for expressing and screening molecules of interest.
    Type: Application
    Filed: April 27, 2012
    Publication date: May 14, 2015
    Applicants: Department of Veterans Affairs, The Board of Trustees of the Leland Stanford Junior University
    Inventors: William H. Robinson, Yann Chong Tan, Jeremy Sokolove
  • Publication number: 20150133308
    Abstract: The present invention relates to a method for improving a repebody protein comprising repeat modules and a nucleotide library encoding a repebody protein library for improving the repebody protein. More particularly, the present invention relates to a method for improving a repebody protein using a module evolution method of sequentially mutating repeat modules constituting the repebody protein, and a nucleotide library encoding a repebody protein library used to improve the protein. According to the module evolution method of the present invention, an improved repebody protein can be screened which has a high binding affinity and accordingly increased specificity and activity, and thus it is easy to express a repebody used as an inhibitor, a therapeutic agent, and an analysis means against a target substance.
    Type: Application
    Filed: November 11, 2014
    Publication date: May 14, 2015
    Inventors: Hak-Sung Kim, Joong-Jae Lee, Hyun-Ho Kyeong, Jung-Min Choi, Da-Eun Hwang, Hae-Kap Cheong, Hyun Jung Kim, Eun-Kyeong Jo, Chul-Su Yang, Jae-Min Yuk
  • Publication number: 20150119264
    Abstract: The invention relates to novel variants that associate with Alzheimer's disease AD and their use in kits as a means for diagnosing AD; and also their use in nucleic acid molecules or cells/cell lines for identifying novel therapeutic, label of identification means.
    Type: Application
    Filed: April 14, 2014
    Publication date: April 30, 2015
    Applicant: University College Cardiff Consultants Limited
    Inventors: Julie Williams, Michael John Owen
  • Publication number: 20150119278
    Abstract: The present invention relates to biomarkers and diagnostic and prognostic methods for Alzheimer's disease and other neurodegenerative disorders. The invention also provides compositions for detecting the biomarker as well as compositions and methods useful for treating Alzheimer's disease and other neurodegenerative disorders.
    Type: Application
    Filed: October 24, 2014
    Publication date: April 30, 2015
    Inventor: Edward J. Goetzl
  • Publication number: 20150111773
    Abstract: The present invention provides methods for rapidly screening and measuring the ligand binding affinity of in vitro selected peptides to the cognate and off-target proteins. This general strategy is amenable to high throughput analysis because the peptides are synthesized by cell-free translation, as opposed to solid-phase synthesis required by traditional assays, and affinities can be readily measured in standard formats.
    Type: Application
    Filed: June 7, 2013
    Publication date: April 23, 2015
    Inventors: John Chaput, Andrew Larsen, Annabelle Gillig
  • Publication number: 20150099643
    Abstract: The invention pertains to a method for diagnosing or detecting lung cancer in human subjects based on ribonucleic acid (RNA) expression, in particular based on RNA from blood. The invention discloses 361 genes which are differentially expressed in blood from lung cancer patients and discloses that at least 4 of the mRNAs must be determined in order to have an AUC of at least 0.8.
    Type: Application
    Filed: July 10, 2014
    Publication date: April 9, 2015
    Inventors: Andrea HOFMANN, Joachim L. SCHULTZE, Jurgen WOLF, Andrea STARATSCHEK-JOX, Thomas ZANDER
  • Publication number: 20150100242
    Abstract: The methods provided focus on a quantitative molecular assay tools that systematically measure a set of pre-selected targets, with proper controls in a biological sample for identification of biomarkers or novel targets for a disease status. This allows for systematically maximizing the power of multivariate feature selection tools on the analysis of high-throughput screening data (such as microarray) and use of the well selected target to generate a qPCR array with tissue specific controls and qPCR controls to serve the needs of biomarker study.
    Type: Application
    Filed: March 15, 2013
    Publication date: April 9, 2015
    Inventors: Xiao Zeng, Song Tian, Jiaye Yu, John Dicarlo, George J. Quellhorst, JR., Vikram Devgan
  • Publication number: 20150087542
    Abstract: The invention concerns a screening method for the detection of Clostridium difficile in a sample, wherein said method comprises the detection of conserved target regions in the Clostridium genome through the binding of at least one oligonucleotide probe to said target site.
    Type: Application
    Filed: May 2, 2013
    Publication date: March 26, 2015
    Applicant: University College Cardiff Consultants Limited
    Inventors: Lesley William James Baillie, Lovleen Tina Joshi
  • Publication number: 20150080252
    Abstract: Compositions and methods are disclosed for identifying agents useful for the treatment of malignancy, particularly GISTs which are resistant to imatinib mesylate (IM). In a preferred embodiment, agents which sensitize cancer cells to IM are provided.
    Type: Application
    Filed: August 25, 2014
    Publication date: March 19, 2015
    Inventors: Andrew K. Godwin, Burton Eisenberg, Yuliya Skorobogatko, Lori Rink, Andrew Kossenkov, Michael F. Ochs
  • Publication number: 20150065359
    Abstract: A 3D organotypic culture which phenocopies aggressive, invasive cancer and methods of use thereof are provided.
    Type: Application
    Filed: September 4, 2014
    Publication date: March 5, 2015
    Inventor: Anil K. Rustgi
  • Publication number: 20150065382
    Abstract: Methods for producing and identifying fragments of proteins, and more particularly to methods for generating and identifying soluble protein domains are disclosed based on a method for generating a library of nucleic acid fragments from nucleic acid encoding a desired polypeptide, and more especially a library of essentially, randomly sampled fragments of coding DNA sequence predominantly of defined size range and a method for selecting cloned gene fragments from the library that encode soluble protein domains.
    Type: Application
    Filed: September 5, 2014
    Publication date: March 5, 2015
    Inventors: Mark McAlister, Renos Savva, Laurence Pearl, Chrisostomos Prodromou, Paul C. Driscoll
  • Publication number: 20150067914
    Abstract: The present invention has increased the resistance to drought stress in Poplar by integrating a transgene constitutively expressing a pine superoxide dismutase (SOD) into the plant genome. It is contemplated that this approach to drought resistance improvement will be equally successful for all woody perennials. Provided with the invention is an expression cassette, a vector, and a method for increasing SOD activity in woody perennials, as well as transgenic woody perennials with enhanced drought resistance and accompanying phenotype.
    Type: Application
    Filed: June 25, 2014
    Publication date: March 5, 2015
    Inventors: Edward G. Kirby, Juan Jesus Molina-Rueda
  • Publication number: 20150057189
    Abstract: Recombinant adeno-associated viral (AAV) capsid proteins are provided. Methods for generating a library of recombinant adeno-associated viral capsid proteins are also provided.
    Type: Application
    Filed: November 10, 2014
    Publication date: February 26, 2015
    Inventors: Mark Kay, Dirk Grimm
  • Publication number: 20150050211
    Abstract: Provided herein are methods to generate and screen peptides that exhibit drug like stabilities in vitro and in vivo. By selecting for enzyme resistance, Applicants are able to derive peptides that are not only stable to a broad spectrum of proteases, but also stable to other drug processing enzymes such as cytochrome P450s. This approach provides a general method to the rapid development of highly stable peptides for therapeutic development and diagnosis. The peptides are further modified for oral bioavailability.
    Type: Application
    Filed: August 31, 2012
    Publication date: February 19, 2015
    Applicant: University of Souththern California
    Inventors: Stephen V. Fiacco, Terry T. Takahashi, Richard W. Roberts
  • Publication number: 20150051094
    Abstract: Diagnostic biomarkers for diagnosing immune suppression/dysfunction. The diagnostic biomarkers are genes and/or transcripts that are up or down regulated compared to normal expression when a subject has been stressed either mentally and/or physically. The invention also relates to a method of detecting comprised or suppressed immune response in a subject by comparing certain diagnostic biomarkers in the subject to a control set of diagnostic biomarkers.
    Type: Application
    Filed: October 20, 2014
    Publication date: February 19, 2015
    Applicant: Government of the United States as represented by the Secretary of the Army
    Inventors: Seid Muhie, Marti Jett, Rasha Hammamieh
  • Publication number: 20150051114
    Abstract: Compositions and methods for the detection and treatment of neurological disorders, including ASD, are provided.
    Type: Application
    Filed: September 29, 2014
    Publication date: February 19, 2015
    Inventors: Hakon Hakonarson, Brett Abrahams, Maja Bucan, Dan Geschwind, Edward Herman, Kai Wang
  • Patent number: 8951755
    Abstract: The use of VHHs in the preparation of products to provide stability of antibody specificity under destabilizing conditions whereby normally lower eukaryotes or traditional antibodies are killed or inactivated.
    Type: Grant
    Filed: March 12, 1999
    Date of Patent: February 10, 2015
    Assignee: BAC IP B.V.
    Inventors: Daniel Convents, Leo G. Frenken, Michael M. Harmsen, Richard H. van der Linden, Cornelis T. Verrips
  • Publication number: 20150038376
    Abstract: The methods provided herein use microarray data for feature selection and then use selected targets to generate industry standard qPCR arrays with new clinical sample assay data so order to build a classification model. This multi-step method overcomes the disadvantages of traditional biomarker identification.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 5, 2015
    Inventors: Song Tian, Xiao Zeng, John Dicarlo, Jiaye Yu, Thomas J. Fahey, Vikram Devgan, George J. Quellhorst, Raymond K. Blanchard
  • Patent number: 8946128
    Abstract: The present invention provides an alternative scaffold for peptides displayed on filamentous phages through novel fusion proteins primarily originating from pIX. Libraries of filamentous phages can be created from fusion proteins, and a phage display system comprising a phagemid and a helper phage is a part of the invention. An aspect of the invention is a kit containing a phage display system comprising a phagemid that contains a nucleic acid encoding the fusion protein of the invention and a helper phage.
    Type: Grant
    Filed: February 24, 2010
    Date of Patent: February 3, 2015
    Assignee: Nextera AS
    Inventor: Geir Åge Løset
  • Publication number: 20150031088
    Abstract: Provided is a chip process of gene synthesis, and the process comprises incorporating the whole procedure, which comprises amplifying oligonucleotides and assembling the oligonucleotides into a gene in parallel, onto a single chip. A specific mismatch endonuclease is also used in the process to establish an error repair system in gene synthesis, and the error rate is decreased to about 0.19 mismatched bases/kb. The high-throughput, high-fidelity and low-cost chip process of gene synthesis provided in the present invention can meet the requirements of gene synthesis and the optimization and screening of protein expression on a large scale at the frontier of life sciences such as synthetic biology, genomics, and systems biology.
    Type: Application
    Filed: April 17, 2012
    Publication date: January 29, 2015
    Inventor: Jingdong Tian
  • Publication number: 20150031585
    Abstract: Described herein are compositions comprising a first aptamer, second aptamer and a target that are capable of forming a ternary complex, and wherein the first aptamer and the second aptamer comprise C-5 pyrimidine modification schemes that are different, and methods of making and using such compositions.
    Type: Application
    Filed: September 24, 2014
    Publication date: January 29, 2015
    Inventors: Urs A. Ochsner, Louis S. Green, Larry Gold, Nebojsa Janjic, Evaldas Katilius
  • Publication number: 20150024971
    Abstract: The present disclosure relates to methods for efficient synthesis, cloning, transformation and screening of large diverse libraries of polynucleotide variants comprising well-defined nucleotide differences relative to a reference polynucleotide.
    Type: Application
    Filed: October 2, 2014
    Publication date: January 22, 2015
    Inventors: Jeffrey Colbeck, Benjamin N. Mijts, Lorraine J. Giver, Richard Fox, Vesna Mitchell, Bumshik Robert Pak, Lynne D. Gilson
  • Publication number: 20150024950
    Abstract: The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.
    Type: Application
    Filed: February 15, 2013
    Publication date: January 22, 2015
    Applicant: Fred Hutchinson Cancer Research Center
    Inventors: Jason H. Bielas, Jessica A. Bertout
  • Publication number: 20150018229
    Abstract: The present invention relates to a polymorphic MRP-1 polynucleotide, genes or vectors comprising the polynucleotides and a host cell genetically engineered with the polynucleotide or gene. Also provided are methods for producing molecular variant polypeptides, cells capable of expressing a molecular variant polypeptide and a polypeptide encoded by the polynucleotide or the gene or obtainable by the method or cells produced herein. Also provided is an antibody to the polypeptide, a transgenic animal, and a solid support comprising one or a plurality of the provided polynucleotides, genes, vectors, polypeptides, antibodies or host cells. Furthermore, methods of identifying a polymorphism, identifying and obtaining a pro-drug or drug or an inhibitor are also provided. In addition, the invention relates to methods for producing a pharmaceutical composition, diagnosing a disease and detection of the polynucleotide.
    Type: Application
    Filed: May 13, 2014
    Publication date: January 15, 2015
    Inventors: Ulrich Brinkmann, Sven Hoffmeyer, Esther Mornhinweg
  • Publication number: 20150018236
    Abstract: Methods for screening libraries of polypeptides for biologically activity on cells. For example, polypeptides can be synthesized and encapsulated along with their coding sequences in microcapsules of an emulsion. Emulsion microcapsules can then be fused with microcapsules comprising test cells and biological activity on the cells is assessed to identify biologically active polypeptides and nucleic acid molecules encoding the same.
    Type: Application
    Filed: February 1, 2013
    Publication date: January 15, 2015
    Applicant: Invenra Inc.
    Inventors: Roland Green, Bryan Glaser, Ivar Meyvantsson, Kimberly Kaufman, Madison Green
  • Publication number: 20150011415
    Abstract: The present disclosure relates to a general method for converting complex gene expression data into a simple, composite disease risk score which can be used for the development of rapid diagnostic tests suitable for clinical use for the determination of the presence of an infection or disease in a host.
    Type: Application
    Filed: February 1, 2013
    Publication date: January 8, 2015
    Inventors: Michael Levin, Myrsini Kaforou, Jethro Herberg, Victoria Wright, Lachlan Coin
  • Publication number: 20150011431
    Abstract: The present disclosure provides humanized antibodies, including antibodies comprising an ultralong CDR3 (e.g. bovine) and uses thereof.
    Type: Application
    Filed: January 9, 2013
    Publication date: January 8, 2015
    Inventors: Vaughn Smider, Omar A. Bazirgan, Hongyuan Helen Mao
  • Publication number: 20150011432
    Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
    Type: Application
    Filed: September 22, 2014
    Publication date: January 8, 2015
    Inventor: Serge Saxonov
  • Publication number: 20150005180
    Abstract: A biochip is provided that suppresses nonspecific adsorption or bonding of a detection target substance without coating with an adsorption inhibitor and that has excellent detection sensitivity. The constitution is such that it has a macromolecular substance containing a phosphorylcholine group and an active ester group on a substrate surface of a biochip substrate.
    Type: Application
    Filed: September 17, 2004
    Publication date: January 1, 2015
    Inventors: Kazuhiko Ishihara, Sohei Funaoka, Kanehisa Yokoyama
  • Patent number: 8921280
    Abstract: An integrated bio-chip includes; a sample detection portion including at least one light receiving device which detects fluorescent light emitted from at least one sample, a light transfer portion disposed on a light incident surface of the sample detection portion, and which includes at least one excitation light absorbing waveguide which absorbs an excitation light and transmits the fluorescent light emitted from the at least one sample, and a sample reaction portion disposed adjacent to an incident end of the at least one excitation light absorbing waveguide, and including at least one reaction region on which the at least one sample is attached, wherein the sample detection portion, the light transfer portion, and the sample reaction portion are integrally coupled to each other as a single component.
    Type: Grant
    Filed: February 11, 2010
    Date of Patent: December 30, 2014
    Assignee: Samsung Electronics Co., Ltd.
    Inventor: Seong-ho Cho
  • Publication number: 20140371091
    Abstract: Biomolecule arrays on a substrate are described which contain a plurality of biomolecules, such as coding nucleic acids and/or isolated polypeptides, at a plurality of discrete, isolated, locations. The arrays can be used, for example, in high throughput genomics and proteomics for specific uses including, but not limited molecular diagnostics for early detection, diagnosis, treatment, prognosis, monitoring clinical response, and protein crystallography.
    Type: Application
    Filed: October 24, 2012
    Publication date: December 18, 2014
    Inventors: Peter Wiktor, Joshua Labaer, Peter Kahn, Bharath Takulapalli, Ji Qiu, Al Brunner, Mitch Magee
  • Publication number: 20140371095
    Abstract: The present invention relates to a novel method for identifying specific marker sequences for diagnosis of prostate cancer and/or for prognosis in prostate cancer, and to the use of the specific marker sequences identified with the aid of this method.
    Type: Application
    Filed: November 14, 2012
    Publication date: December 18, 2014
    Applicant: Protagen AG
    Inventors: Peter Amersdorfer, Angelika Lueking, Axel Kowald, Bettina Schlick, Petra Massoner, Christof Seifart, Georg Schäfer, Helmut Klocker, Peter Schulz-Knappe, Klaus Marquart
  • Publication number: 20140364340
    Abstract: The present invention overcomes the inadequacies inherent in the known methods for generating libraries of antibody-encoding polynucleotides by specifically designing the libraries with directed sequence and length diversity. The libraries are designed to reflect the preimmune repertoire naturally created by the human immune system and are based on rational design informed by examination of publicly available databases of human antibody sequences.
    Type: Application
    Filed: January 8, 2014
    Publication date: December 11, 2014
    Applicant: ADIMAB, LLC
    Inventors: Maximiliano Vasquez, Michael Feldhaus, Tillman U. Gerngross, K. Dane Wittrup
  • Publication number: 20140364336
    Abstract: This invention provides transcription regulatory control sequences, the activity of which function as biomarkers for a variety of biological responses. This invention also provides expression constructs in which a biomarker transcription regulatory sequence is operably linked with a sequence for a reporter. Cells that comprise these expression constructs can be used in assays to identify conditions that modulate activity of the biological response.
    Type: Application
    Filed: July 13, 2014
    Publication date: December 11, 2014
    Applicant: SWITCHGEAR GENOMICS, INC.
    Inventors: Shelley Force Aldred, Nathan D. Trinklein, Michael Rose, Patrick Collins
  • Publication number: 20140364325
    Abstract: A sensor array includes resonator sensors having respective receptor materials disposed thereon. The receptor materials have a physical property relevant to their ability to bind or adsorb one or more analytes in a sample. The physical property of the receptor materials on the sensors systematically increases or decreases in degree from one sensor to the next in the array. The device also comprises at least one detector for detecting sensor responses when masses of the analytes are adsorbed or bound to the receptor materials on the sensors. With this graded panel of sensors in the array, the analytes may adsorb or bind to the functionalized sensors with a pattern of responses specific to each analyte.
    Type: Application
    Filed: June 5, 2013
    Publication date: December 11, 2014
    Inventors: Michael D Cable, Heinrich Sussner
  • Patent number: 8901045
    Abstract: Methods useful in constructing libraries that collectively display and/or express members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods are disclose. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens are also disclosed.
    Type: Grant
    Filed: May 4, 2012
    Date of Patent: December 2, 2014
    Assignee: Dyax Corp.
    Inventors: Robert C. Ladner, Edward H. Cohen, Horacio G. Nastri, Kristin L. Rookey, Rene Hoet, Hendricus Renerus Jacobus Mattheus Hoogenboom