Solid-phase Synthesis (i.e., Wherein One Or More Library Building Blocks Are Bound To A Solid Support During Library Creation) Or Particular Method Of Cleavage From The Solid Support Used Patents (Class 506/30)
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Publication number: 20110065611Abstract: A method of fabricating a device comprising an array of test locations defined on a substrate with each test location comprising one or more identical oligonucleotides affixed to the substrate.Type: ApplicationFiled: July 8, 2010Publication date: March 17, 2011Inventors: Cornelis Jacobus Maria Heemskerk, Stefan Matthias Matysiak, Johannes Martinus Lambertus Maria Van Helvoort
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Publication number: 20110059869Abstract: An object of the invention is to provide a method for increasing enzymatic reactivity to a target substance immobilized on a support; and a method for reducing or suppressing an inhibitory effect of a support on enzymatic reaction. The above object is achieved by a method for increasing enzymatic reactivity to a target substance immobilized on a support by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist; and a method for reducing or suppressing an inhibitory effect of a support immobilized with a target substance on enzymatic reactivity to the target substance by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist.Type: ApplicationFiled: February 27, 2009Publication date: March 10, 2011Inventors: Miki Kojima, Piero Carninci, Yoshihide Hayashizaki, Matthias Harbers
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Publication number: 20110045994Abstract: The present invention provides a device and methods of use thereof in microscale particle capturing and particle pairing. This invention provides particle patterning device, which mechanically traps individual particles within first chambers of capture units, transfer the particles to second chambers of opposing capture units, and traps a second type of particle in the same second chamber. The device and methods allow for high yield assaying of trapped cells, high yield fusion of trapped, paired cells, for controlled binding of particles to cells and for specific chemical reactions between particle interfaces and particle contents. The device and method provide means of identification of the particle population and a facile route to particle collection.Type: ApplicationFiled: February 11, 2009Publication date: February 24, 2011Inventors: Joel Voldman, Alison M. Skelley, Oktay Kirak, Rudolf Jaenisch
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Publication number: 20110046005Abstract: Provided are methods and nucleic acids for detecting, differentiating or distinguishing between colon cell proliferative disorders by analysis of one or more of the genes Versican, TPEF, H-Cadherin, Calcitonin, and EYA4. Further provided are novel nucleic acid sequences useful for the cell proliferative disorder specific analysis of said genes as well as methods, assays and kits thereof.Type: ApplicationFiled: June 4, 2010Publication date: February 24, 2011Applicant: EPIGENOMICS AGInventors: PETER ADORJAN, MATTHIAS BURGER, SABINE MAIER, RALF LESCHE, SUSAN COTTRELL, SUZANNE MOONEY
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Publication number: 20110046018Abstract: This invention provides novel compositions and methods for the detection, and/or quantification, of the presence and/or activity of one or more kinases and/or phosphatases. In certain embodiments this invention a device for the detection of kinase and/or phosphatase activity where the device comprises a Raman active surface comprising features that enhance Raman scattering having attached thereto a plurality of kinase and/or phosphatase substrate molecules.Type: ApplicationFiled: December 23, 2008Publication date: February 24, 2011Inventors: Fanqing Frank Chen, Gang L. Liu, Jonathan A. Ellman
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Publication number: 20110028350Abstract: Novel compounds are provided, which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Y, wherein Y is a chemical structure as shown in FIG. 5. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.Type: ApplicationFiled: July 28, 2009Publication date: February 3, 2011Applicant: Affymetrix, INC.Inventors: Glenn H. McGall, Anthony D. Barone
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Patent number: 7858375Abstract: The present invention relates to methods for fabricating air-stable supported lipid bilayer membranes. In one embodiment, the present invention relates to methods of producing supported lipid bilayer membranes stabilized by sterol groups that are covalently tethered to a solid surface. In a further embodiment, the present invention relates to air-stable supported lipid bilayer membranes produced by the methods of the present invention.Type: GrantFiled: March 17, 2008Date of Patent: December 28, 2010Assignee: MicroSurfaces, IncInventors: Xiaoyang Zhu, Yang Deng, Yini Wang, David Barriet, Athena Guo
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Publication number: 20100323912Abstract: The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions, and for determining various characteristics of the different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to stimulate, enhance, or inhibit such reactions.Type: ApplicationFiled: June 11, 2010Publication date: December 23, 2010Applicant: Pacific Biosciences of California, Inc.Inventors: Jonas Korlach, Stephen Turner, Benjamin Flusberg, Mark Chaisson, Eric Schadt, Jeffrey Wegener
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Publication number: 20100317916Abstract: The present invention is directed to methods for determining the presence or absence of a genetic defect in an IVF embryo prior to transfer comprising performing real-time PCR and 2???CT analyses to determine normalized copy number of at least one invariant locus on at least one chromosome collected from at least one cell of the embryo and selecting a candidate IVF embryo determined to be without genetic defect for transfer.Type: ApplicationFiled: June 11, 2010Publication date: December 16, 2010Inventors: Richard T. Scott, JR., Nathan R. Treff
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Publication number: 20100304994Abstract: Novel methods for making high resolution oligonucleotide paints are provided. Novel, high resolution oligonucleotide paints are also provided.Type: ApplicationFiled: May 14, 2010Publication date: December 2, 2010Applicant: President and Fellows of Havard CollegeInventors: Chao-ting Wu, George M. Church, Benjamin Richard Williams
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Publication number: 20100298163Abstract: A microfluidic system for fluid transfer to a microarray includes a liquid transfer needle having a fluid conduit therein within which is defined a withholding pressure P1, and a microcompartment defined within the microarray, the microcompartment being configured to generate a capillary pressure P2 therein. The capillary pressure P2 is less than the withholding pressure P1, such that a defined amount of liquid is transferred from the liquid transfer needle into the microcompartment when the liquid transfer needle and the microcompartment are disposed in fluid flow communication. A method of delivering multiple solutions to a plurality of microcompartments in an microarray while avoiding cross-contamination between the solutions is also provided.Type: ApplicationFiled: January 5, 2009Publication date: November 25, 2010Applicant: THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING/MCGILL UNIVERSITYInventors: David Juncker, Mateu Pla
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MICRO-PATTERNED PLATE COMPOSED OF AN ARRAY OF RELEASABLE ELEMENTS SURROUNDED WITH SOLID OR GEL WALLS
Publication number: 20100285992Abstract: A micro-patterned plate composed of an array of releasable elements surrounded by a gel or solid wall and methods of manufacture of the micropatterned plate. The surface properties of walls can be tailored if needed to be repellent or attracting to proteins. The walls can also inhibit cell attachment. The walls enable cells or other materials to be localized to the tops of the releasable elements. The individual element in the array of releasable elements can be released from the array by a mechanical force.Type: ApplicationFiled: July 25, 2008Publication date: November 11, 2010Inventors: Yuli Wang, Nancy Allbritton -
Publication number: 20100279899Abstract: The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention.Type: ApplicationFiled: May 5, 2010Publication date: November 4, 2010Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Mack J. Fulwyler, Joe W. Gray
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Patent number: 7825070Abstract: A novel method for two-dimensionally arraying ferritin on a substrate is provided which obviates the need for a metal ion that permits linking between adjacent two ferritin particles. In a method of two-dimensionally arraying ferritin on a substrate, the surface of the substrate is hydrophilic, and the method includes the steps of: developing a solution containing a solvent and the ferritin on the substrate; and removing the solvent from the solution developed on the substrate, while the ferritin has an amino acid sequence set out in SEQ ID NO: 1 modified at its N-terminus.Type: GrantFiled: November 13, 2008Date of Patent: November 2, 2010Assignee: Panasonic CorporationInventors: Takuro Matsui, Nozomu Matsukawa, Kazuaki Nishio
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Publication number: 20100273681Abstract: Methods for synthesizing chain molecules on particles in a multi-stream laminar flow, microfluidic reaction cells in which the methods can be carried out, and microfluidic systems incorporating the microfluidic reaction cells are provided. The methods, cells and systems are well suited for the rapid, large-scale production of chain biomolecules, such as oligonucleotides, in parallel.Type: ApplicationFiled: April 27, 2009Publication date: October 28, 2010Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Franco Cerrina, Tao Wang
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Publication number: 20100273662Abstract: The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5? ends and at their 3? ends.Type: ApplicationFiled: April 20, 2010Publication date: October 28, 2010Inventors: Niall Anthony Gormley, Geoffrey Paul Smith, David Bentley, Roberto Rigatti, Shujun Luo
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Publication number: 20100273670Abstract: Embodiments of the present invention relate to compositions, methods and apparatus for detection of antiviral agent resistance or sensitivity of a virus. In some embodiments, antiviral agent resistance or sensitivity of influenza types, such as A, B and C may be identified. In more embodiments, sub-types such as the hemagglutinin (HA) and neuraminidase (NA) of influenza A may be analyzed for antiviral agent resistance or sensitivity. In a particular embodiment, the various strains of influenza virus may be analyzed for resistance or sensitivity using DNA microarray analysis designed to target a gene segment. In various embodiments, a microarray-based assay system with capture and detection (label) probes may be utilized to identify a change in a gene segment that confers resistance or sensitivity to an antiviral agent of an influenza strain.Type: ApplicationFiled: August 21, 2007Publication date: October 28, 2010Inventors: Kathy L. Rowlen, Robert Kuchta, Michael Townsend, James Smagala, Erica Dawson
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Publication number: 20100267589Abstract: A liquid discharging device includes a plurality of liquid discharge sections. Each liquid discharge section includes a reservoir, a nozzle that discharges a solution supplied from the reservoir, and discharge energy generating means that generates energy to discharge the solution from the nozzle. The number of the liquid discharge sections corresponds to the number of probe types to be formed. The nozzles are two-dimensionally arranged. Using this liquid discharging device, probe liquids are discharged from the corresponding reservoirs onto a solid-phase substrate to form a predetermined two-dimensional probe array of high-purity probes on the substrate. This process exhibits high reproducibility and processability, and the resulting probe array has high array density.Type: ApplicationFiled: March 12, 2010Publication date: October 21, 2010Applicant: CANON KABUSHIKI KAISHAInventors: Tadashi Okamoto, Nobuko Yamamoto, Hidenori Watanabe, Tomohiro Suzuki
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Publication number: 20100267572Abstract: The disclosure features a collection that comprises a plurality of polymers, typically nucleic acid molecules in a compact form. The molecules include all possible sequences or at least a certain percentage of all possible sequences, of a particular length.Type: ApplicationFiled: June 28, 2010Publication date: October 21, 2010Applicant: THE BRIGHAM AND WOMENS HOSPITAL, INC.Inventors: Martha L. Bulyk, Anthony A. Philippakis, Preston Wayne Estep
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Publication number: 20100260745Abstract: The present invention relates to the use of nanowires, nanotubes and nanosensor platforms. In one embodiment, the present invention provides a method of constructing a nanosensor platform. In another embodiment, the present invention provides a method of analyzing multiple biomarker signals on a nanosensor platform for the detection of a disease.Type: ApplicationFiled: October 1, 2008Publication date: October 14, 2010Applicant: UNIVERSITY OF SOUTHERN CALIFORNIAInventors: Chongwu Zhou, Mark E. Thompson, Richard James Cote, Fumiaki Ishikawa, Marco Curreli, Hsiao-Kang Chang
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Publication number: 20100261621Abstract: Provided are a microarray substrate comprising a solid substrate coated with a chemical having a functional residue represented by Formula 1 or 2 below, a method of analyzing a biomolecule using the microarray substrate, and a lab-on-a-chip comprising the microarray substrate: wherein n, the structure within brackets [ ], R1, R2, R3, R10, n and l are as defined in the specification.Type: ApplicationFiled: June 28, 2010Publication date: October 14, 2010Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventors: Sang-hyun PEAK, Jong-myeon PARK, Chang-eun YOO
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Publication number: 20100256016Abstract: A biomolecular assay includes a substrate with a metallic layer on at least one surface thereof. The metallic film includes nanocavities. The nanocavities are configured to enhance signals that are representative of the presence or amount of one or more analytes in a sample or sample solution, and may be configured to enhance the signal by a factor of about two or more or by a factor of about three or more. Such signal enhancement may be achieved with nanocavities that are organized in an array, randomly positioned nanocavities, or nanocavities that are surrounded by increased surface area features, such as corrugation or patterning, or nanocavities that have quadrilateral or triangular shapes with tailored edge lengths, or with a plurality of nanoparticles. Methods for fabricating biomolecular substrates and assay techniques in which such biomolecular substrates are used are also disclosed.Type: ApplicationFiled: August 2, 2006Publication date: October 7, 2010Applicant: University of Utah Research FoundationInventors: Steven M. Blair, Farhad Mahdavi, Yongdong Liu, James N. Herron, Ajay Nahata
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Publication number: 20100248992Abstract: Methods and apparatuses for selecting and arranging clinically relevant chromosomal loci allow an exemplary diagnostic array to simultaneously test for numerous genetic alterations that occur in many different parts of the human genome. Clinically irrelevant or ineffective loci are eliminated. One implementation increases reliability and accuracy by dividing the base-pair sequence of each chromosomal locus into segments and then assigning nucleic acid clones for comparative genomic hybridization to each different segment. The segments may overlap for increased resolution and control. Clones representing segments that are adjacent on a native chromosome are placed in non-adjacent target areas of the array to avoid interfering hybridization reactions. Arrangement motifs within an array may be redundantly repeated for high availability and increased reliability and accuracy of results. Techniques, hardware, software, logic engines, loci collections, and diagnostic arrays are described.Type: ApplicationFiled: June 10, 2010Publication date: September 30, 2010Applicant: SIGNATURE GENOMICS LABORATORY LLCInventors: Lisa G. Shaffer, Bassem A. Bejjani
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Publication number: 20100248993Abstract: This invention provides a method for protein patterning and fabrication of biomolecule microarrays, based on the selective biomolecule adsorption on hydrophilic versus hydrophobic patterns created by selective plasma deposition of fluorocarbon film.Type: ApplicationFiled: June 20, 2008Publication date: September 30, 2010Inventors: Angeliki Tserepi, Evangelos Gogolides, Sotirios Kakabakos, Panagiota Petrou, Pinelopi Bayiati, Evrimahos Matrozos
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Publication number: 20100227771Abstract: Methods and tools (e.g., kits, articles of manufacturing, support and arrays) for the solid-phase detection of a target molecule using a cationic polymer and nucleic acid probe complex is provided herewith. These methods and tools allows for the reagentless, ultrasensitive and specific detection of nucleic acids, proteins and other molecules of interest and are based on a labeled complex made of specific capture probes and a polythiophene derivative.Type: ApplicationFiled: May 11, 2007Publication date: September 9, 2010Applicant: Universite LavalInventors: Ahmed Najari, Hoang-Anh Ho, Mario Leclerc
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Publication number: 20100210478Abstract: The present invention relates to quantitative and quantity aspects of array synthesis and array uses as a device for high capacity producing synthetic molecules for off-array surface applications and as an assay device for on-array surface applications.Type: ApplicationFiled: June 29, 2007Publication date: August 19, 2010Inventors: Xiaolian Gao, Kiaochun Zhou, Xiaolin Zhang, Ailing Hong, Qi Zhu, Peilin Yu
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Publication number: 20100210474Abstract: Disclosed are heterocyclic scaffolds useful, for example, for solid-phase organic synthesis of combinatorial libraries and methods for the preparation thereof. Also disclosed are libraries, including combinatorial libraries, and methods for preparation thereof.Type: ApplicationFiled: September 16, 2008Publication date: August 19, 2010Inventor: Gary Gellerman
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Publication number: 20100210469Abstract: The present invention relates to techniques to detect chromosomal columns abnormalities. More specifically, the present invention is directed to a microarray chip for detecting chromosomal abnormalities comprising one or more pooled probe sets, wherein the pooled probe set is specific to a chromosomal abnormality and all probes of each pooled probe set are immobilized together in at least one spot; a method of detecting chromosomal abnormalities using the microarray chip; a kit for diagnosing diseases associated with chromosomal abnormalities comprising the microarray chip; and a method of diagnosing a disease associated with a chromosomal abnormality by identifying the chromosomal abnormality specific to the disease using the microarray chip. The present invention relates to techniques to detect chromosomal abnormalities.Type: ApplicationFiled: September 7, 2007Publication date: August 19, 2010Applicant: MACROGEN INC.Inventors: Jason Jongho Kang, Eun-Hee Oh, Hyun-Wong Kang, Jung-Wan Lee, Seung-Heui Lee, Song-Ju Yang, Jeong-Sun Seo, Jong-Ho Lee, Kap-Seok Yang
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Publication number: 20100204056Abstract: A method for fabricating and assessing the quality of printing on substrates used for chemical or biological array testing, and method for ascertaining relative consistency of the deposit reproducibility is described. Generally, the method involves: providing a substrate, providing a solution mixture of a capture moiety and a colorant; depositing an amount of said solution mixture onto said substrate, monitoring the substrate such that the visible indicator colorant manifests in a co-extensive fashion as with the capture moiety on the substrate. In another aspect, the invention relates to a sensor or assay device comprising a physical complex of a number of capture agents and an amount of colorants adhere to least a part of a surface of a substrate.Type: ApplicationFiled: December 18, 2009Publication date: August 12, 2010Inventors: James M. Takeuchi, Shawn R. Feaster
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Publication number: 20100204065Abstract: A microarray including hydrogel and a plurality of probes which are immobilized in discrete regions of the hydrogel, and a method of preparing the same are provided. When using the microarray and method, a solid substrate is not required and many biomolecules can be immobilized in a small volume, thereby obtaining high sensitivity. Since gel can be cut to obtain many pieces, many microarrays can be prepared at once.Type: ApplicationFiled: April 19, 2010Publication date: August 12, 2010Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventors: Su-hyeon KIM, In-ho LEE, Jun-hong MIN
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Publication number: 20100204064Abstract: An integrated bio-chip includes; a sample detection portion including at least one light receiving device which detects fluorescent light emitted from at least one sample, a light transfer portion disposed on a light incident surface of the sample detection portion, and which includes at least one excitation light absorbing waveguide which absorbs an excitation light and transmits the fluorescent light emitted from the at least one sample, and a sample reaction portion disposed adjacent to an incident end of the at least one excitation light absorbing waveguide, and including at least one reaction region on which the at least one sample is attached, wherein the sample detection portion, the light transfer portion, and the sample reaction portion are integrally coupled to each other as a single component.Type: ApplicationFiled: February 11, 2010Publication date: August 12, 2010Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventor: Seong-ho CHO
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Publication number: 20100204063Abstract: A DNA chip package includes; a base substrate, a photoresist layer disposed on the base substrate, at least one DNA chip mounting groove disposed in the photoresist layer and exposing the base substrate therethrough, and at least one DNA chip mounted in the at least one DNA chip mounting groove.Type: ApplicationFiled: November 16, 2009Publication date: August 12, 2010Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventor: Tae-seok SIM
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Patent number: 7772159Abstract: The invention provides a new process for the preparation of polypyrrolinones (I) of various sizes which have found to be useful peptidomimetics. One aspect of the invention is a new process utilizing ?-amino-?-substituted-valerolactones as synthons. A second aspect of the invention is a process for the synthesis of polypyrrolinones using solid-phase techniques.Type: GrantFiled: July 20, 2004Date of Patent: August 10, 2010Assignee: The Trustees of the University of PennsylvaniaInventors: Amos B. Smith, III, Hu Liu, Hiroyuki Ikumura, Ralph F. Hirschmann
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Patent number: 7771929Abstract: Families of compositions are provided as labels, referred to as eTag reporters for attaching to polymeric compounds and assaying based on release of the eTag reporters from the polymeric compound and separation and detection. For oligonucleotides, the eTag reporters are synthesized at the end of the oligonucleotide by using phosphite or phosphate chemistry, whereby mass-modifying regions, charge-modifying regions and detectable regions are added sequentially to produce the eTag labeled reporters. By using small building blocks and varying their combination large numbers of different eTag reporters can be readily produced attached to a binding compound specific for the target compound of interest for identification. Protocols are used that release the eTag reporter when the target compound is present in the sample.Type: GrantFiled: April 18, 2003Date of Patent: August 10, 2010Assignee: Monogram Biosciences, Inc.Inventors: Sharat Singh, Tracy Matray, Hossein Salimi-Moosavi
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Publication number: 20100197524Abstract: Improved electrochemical biosensor arrays and instruments are disclosed herein. Methods of making and using the electrochemical biosensor instruments are also disclosed. An electrochemical biosensor array can include an array of microelectrodes disposed on a substrate. Each microelectrode can include a conducting electrode material disposed on a portion of the substrate, a first polymeric layer disposed on at least a portion of the conducting electrode material, a second polymeric layer disposed on at least a portion of the first polymeric layer, and a capture molecule that is in physical communication with the second polymeric layer.Type: ApplicationFiled: July 31, 2008Publication date: August 5, 2010Applicant: GEORGIA TECH RESEARCH CORPORATIONInventors: Jiri Janata, Miroslava Josowicz, George Yang Yu
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Publication number: 20100190654Abstract: A nanoarray includes a fluoropolymer array defining a plurality of cavities where each cavity has a predetermined shape and is less than about 5 micrometers in a broadest cross-sectional dimension. The nanoarray also includes a composition discretely contained in each cavity, where the composition includes a linking group for coupling with a modifying group. The nanoarray can be fabricated from fluoropolyether or perfluoropolyether.Type: ApplicationFiled: December 5, 2007Publication date: July 29, 2010Applicant: Liquidia Technologies , Inc.Inventors: Ginger D. Rothrock, Robert L. Henn
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Publication number: 20100184622Abstract: A substrate for biochips with which immobilization is easy, which does not exhibit self-fluorescence, which is easy to manufacture, and which is excellent in flatness and surface precision, is disclosed. A substrate having a substrate body of the biochip, which is made of a metal, and a carbon layer having functional groups formed on the metal substrate body is used as a substrate for biochips. Since the substrate body of the substrate for biochips is made of a metal, the substrate is not only easy to manufacture, but also free from cracking and chipping, so that it allows easy handling, and high flatness and surface precision can be attained. Therefore, the problem that the optical system is hard to focus when detecting fluorescence does not occur. Moreover, since the substrate body is made of a metal, it does not emit fluorescence by itself. In addition, since the carbon layer has functional groups such as amino groups, biologically relevant substances can be easily immobilized.Type: ApplicationFiled: May 24, 2005Publication date: July 22, 2010Inventors: Kiyoshi Nokihara, Yasuo Oka
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Publication number: 20100185047Abstract: Disclosed are arrays of nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods using SNPs on genes of the bovine interferon tau signaling pathway for improved bovine fertilization rate, or embryo survival, or both.Type: ApplicationFiled: December 15, 2009Publication date: July 22, 2010Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION (WARF)Inventor: Hasan Khatib
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Publication number: 20100179071Abstract: A method for producing a cell pattern is provided, comprising the step of forming a hydrophobic film on a substrate, patterning the hydrophobic film by a laser beam, transferring the hydrophobic film from the substrate to a medium, and culturing cells on the medium to form a cell pattern.Type: ApplicationFiled: May 19, 2009Publication date: July 15, 2010Applicant: NATIONAL TAIWAN UNIVERSITYInventors: Chin-Hsiung Hsieh, Yi-You Huang
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Patent number: 7754646Abstract: Highly functionalized, porous organosilica particles and methods of their synthesis are described that employ high amounts of functional silane such as 3-mercaptopropyl trimethoxysilane. Silane particle diameters are controlled from less than 1 ?m to over 100 ?m. The particles have a high surface area due to their advantageous internal structures, which consist of large pores, typically up to 10 ?m that are linked by small channels of typically about 20 nm diameter. Isothiocyanate modified fluorescent dyes can enter and react with thiol groups inside the pores. The invention also provides clear silica shells of controlled thicknesses to protect fluorescent signals and provide an independent parameter for distinguishing particle types based on light scattering off different sized particles. The particles are stable and useful for many purposes, particularly for optical bar coding in combinatorial synthesis of polymers such as nucleic acid, polypeptide, and other synthesized molecules.Type: GrantFiled: July 1, 2002Date of Patent: July 13, 2010Assignee: Nanomics Biosystems Pty LtdInventors: Mathias Trau, Angus Johnston
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Publication number: 20100173803Abstract: The invention provides an ink jet device and method for producing a biological assay substrate (41) by releasing a plurality of substances onto the substrate (41) The device comprises at least a print head and a plurality of substance containers (60, 61, 62) connectable thereto, and mounting means (55) for the containers, whereby at least part of the containers (60, 61, 62) is provided with identification means (63) The device further comprises reading means to read information contained in the identification means (63).Type: ApplicationFiled: August 10, 2007Publication date: July 8, 2010Applicant: KONINKLIJKE PHILIPS ELECTRONICS N.V.Inventors: Anke Pierik, Johan Frederik Dijksman, Martin Maurice Vernhout, Leonardus Johannes Cornelius Van Den Besselaar, Dirkjan Bernhard Van Dam, Giovanni Nisato, Albert Hendrik Jan Immink
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Patent number: 7749940Abstract: A novel tagged triazine library is provided in which three building blocks are prepared separately and assembled orthogonally to yield 1536 highly pure compounds. Each library compound contains a variety of a triethyleneglycol (TG) linker at one of the diversity sites of the triazine scaffold.Type: GrantFiled: May 5, 2004Date of Patent: July 6, 2010Assignee: New York UniversityInventor: Young-Tae Chang
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Publication number: 20100167958Abstract: Methods for optically trapping and manipulating micro- and nano-sized particles by using light to induce localized surface plasmon resonance on metallic surface of a substrate. The method includes the steps of contacting a substrate with a medium having particles suspended therein; focusing a beam of coherent light onto the substrate such that the beam induces surface plasmon resonance; and trapping at least one of the suspended particles using a light induced dielectrophoresis force generated by the surface plasmon resonance.Type: ApplicationFiled: June 18, 2007Publication date: July 1, 2010Applicant: WASHINGTON, UNIVERSITY OFInventors: Lih-Yuan Lin, Xiaoyu Miao, Suzie Pun
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Publication number: 20100167955Abstract: A microarray with excellent sensitivity for detecting target materials during biological assays, and a method of manufacturing the same.Type: ApplicationFiled: July 28, 2009Publication date: July 1, 2010Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventor: Sun-ok Jung
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Publication number: 20100167941Abstract: The invention provides a particle comprising a surface, wherein a portion of the surface is capable of emitting electromagnetic radiation and another portion of the surface is capable of emitting a differential electromagnetic radiation (either different intensity, different frequency or no radiation), and wherein the arrangement of said portions of the surface defines a spatially distributed code for identifying the particle. The invention also provides method of manufacturing a particle having an identifying code comprising providing a particle with a functionalized surface which comprises functional binding moieties and selectively removing a plurality of the functional binding moieties from the surface to create a pattern of functionalized and differentially-functionalized zones on the surface. Various liquid-based assay methods employing the particle of the invention and a kit comprising the particle of the invention are also disclosed.Type: ApplicationFiled: August 11, 2009Publication date: July 1, 2010Inventors: Nigel G. Skinner, Paul Fenton, George A. Adaniya
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Publication number: 20100160183Abstract: Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.Type: ApplicationFiled: June 6, 2008Publication date: June 24, 2010Inventors: Tao Xu, James J. Yoo, Anthony Atala
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Publication number: 20100152052Abstract: The present invention relates to specimens for use in microanalysis processes. One aspect of the invention is directed toward using a mold to form specimens for a microanalysis process (e.g., including an atom probe and/or transmission electron microscope processes). Other aspects of the invention are directed towards embedding specimen material (e.g., including nanoparticles) in an embedment material to produce a specimen suitable for use in a microanalysis process. Still other aspects include combining specimen material with an embedment material to enhance a microanalysis process. Yet other embodiments of the invention are directed toward combining a specimen material with multiple embedment materials to produce specimens suitable for a microanalysis process. Further aspects of the invention are directed toward analyzing at least a portion of a specimen produced by one or more of the processes discussed above.Type: ApplicationFiled: July 28, 2006Publication date: June 17, 2010Inventors: Steven L. Goodman, Thomas F. Kelly, Terri J. Tomicki
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Patent number: 7737088Abstract: The present invention relates to arrays of biochemical and/or biofunctional elements such as nucleic acids (oligonucleotides, for example) or other biomolecules on a carrier surface and methods of producing such arrays using photoactivation of predetermined areas for synthesis using an illumination matrix that is computer-controlled to generate an exposure pattern. This exposure pattern can be adjusted and monitored by computer using a light sensor matrix, for example a CCD matrix, to allow precise, controlled illumination of specific regions and therefore attachment of array building blocks to those specific regions. The methods and compositions of the invention permit spatially resolved photochemical synthesis of polymer probes on a carrier.Type: GrantFiled: August 27, 1999Date of Patent: June 15, 2010Assignee: Febit Holding GmbHInventors: Cord F. Stähler, Peer F. Stähler, Manfred Müller, Fritz Stähler, Hans Lindner
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Publication number: 20100144556Abstract: An embodiment can be the use of motor proteins for cargo loading and transport in nano-devices and systems. One embodiment of the use of motor proteins can be adding biotin-binding proteins to a substrate by patterning, binding biotinylated F-actin to the biotin-binding proteins, aligning the bound F-actin in a preferred direction using a flow field, and using myosin coated particles to transport items attached to the particle throughout the substrate. Another embodiment of the use of motor proteins can be adding biotin-binding proteins to a substrate by patterning, adding a flow field, injecting F-actin so that the F-actin is bound and aligned simultaneously, and using myosin coated particles to transport items attached to the particle throughout the substrate. In either embodiment the F-actin can be capped with a biotinylated cap before binding to the biotin-binding proteins.Type: ApplicationFiled: December 4, 2009Publication date: June 10, 2010Inventors: Parviz Famouri, Yongkuk Lee, Lenln Leon
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Publication number: 20100144555Abstract: The invention concerns a method for identifying and/or cloning nucleic acid regions representing qualitative differences associated with alternative splicing events and/or with insertions, deletions located in RNA transcribed genome regions, between two physiological situations, comprising either hybridization of RNA derived from the test situation with cDNA's derived from the reference situation and/or reciprocally, or double-strand hybridization of cDNA derived from the test situation with cDNA's derived from the reference situation; and identifying and/or cloning nucleic acids representing qualitative differences. The invention also concerns compositions or banks of nucleic acids representing qualitative differences between two physiological situations, obtainable by the above method, and their use as probe, for identifying genes or molecules of interest, or still for example in methods of pharmacogenomics, and profiling of molecules relative to their therapeutic and/or toxic effects.Type: ApplicationFiled: December 17, 2009Publication date: June 10, 2010Applicant: ExonHit TherapeuticsInventors: Bruno TOCQUE, Laurent Bracco, Florence Edon, Fabien Schweighoffer