Oxytocin; Vasopressin; Related Peptides Patents (Class 530/315)
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Patent number: 5763405Abstract: A solid pharmaceutical composition for oral administration of small and medium size peptides, particularly vasopressin, oxytocin, and their analogues, comprises said peptide, an enteric coat and a pharmaceutically acceptable carrier containing a buffering agent buffering at a pH of from 2 to 6, preferably at about pH 5. A method of manufacture of single doses of said peptide comprises mixing of the ingredients, forming the resulting mixture into spheres smaller than 2 mm, coating the spheres with an enteric coat which is readily soluble in gastric juice of pH 5.0 or higher but not at substantially lower pH, and filling the coated spheres in capsules or incorporating them into tablets, degradable in the stomach. Also disclosed is a method for oral administration to a patient of said single dose.Type: GrantFiled: October 23, 1996Date of Patent: June 9, 1998Assignee: Ferring B.V.Inventors: Anne Fjellestad-Paulsen, Christina Ahlm-Soderberg
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Patent number: 5756344Abstract: Disclosed are (1) a DNA containing a cDNA segment coding for human endothelin-2 (SEQ ID NO:1), (2) a precursor of human endothelin-2 (SEQ ID NO:2), (3) a transformant carrying a DNA containing a cDNA segment coding for human endothelin-2, and (4) a method for preparing mature human endothelin-2 which comprises culturing the transformant described in (3), accumulating a protein in a culture medium, and collecting the same, whereby human endothelin-2 and the precursors thereof can be produced in large amounts.Type: GrantFiled: June 28, 1996Date of Patent: May 26, 1998Assignee: Takeda Chemical Industries, Ltd.Inventors: Haruo Onda, Shoichi Ohkubo, Takuo Kosaka
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Patent number: 5726287Abstract: A process for preparing and purifying cyclic peptides having disulfide moieties in a single processing operation which simplifies synthesis and reduces production costs, yet produces high, quality yield. Higher yields are obtained by isolating the desired cyclic compound through direct ion exchange chromatography as an integral part of the single process. The improved process is particularly useful for the preparation of vasopressin and oxytocin and their respective derivatives and analogs.Type: GrantFiled: June 20, 1996Date of Patent: March 10, 1998Assignee: Ferring ABInventors: Lars Henrik Harald Andersson, Jan-Ake Skoldback
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Patent number: 5698516Abstract: Vasopressin analogues of formula (I) ##STR1## wherein X is (S)-2-amino-2-methyl-butanoic acid (CaMeAbu) or Valine (Val), Y is Thienylalanine (Thi) or Methionine (Met), Z is D-Phenylalanine (D-Phe) or D-Thienylalanine (Thi) or D-Tyrosine (D-Tyr), are disclosed. Pharmaceutical preparations comprising a vasopressin analogue of the invention as active ingredient are disclosed and exemplified by oral preparations, nasal preparations, and intravenous preparations. The vasopressin analogues of the invention are intended for use as a medicament, specially an antidiuretic agent. The antidiuretic agent is preferably used for the treatment of diabetes insipidus or enuresis.Type: GrantFiled: December 18, 1995Date of Patent: December 16, 1997Assignee: Ferring B.V.Inventors: Anders Nilsson, H.ang.kan Olson, Christina Soderberg-Ahlm, Jerzy Trojnar
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Patent number: 5629172Abstract: The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.Type: GrantFiled: August 2, 1994Date of Patent: May 13, 1997Assignee: Celtrix Pharmaceuticals, Inc.Inventors: Desmond Mascarenhas, Yang Zhang, Pamela S. Olson, David R. Olsen, Pedro A. Cohen
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Patent number: 5627268Abstract: The present invention relates to a novel method for making a predetermined, desired peptide in transgenic animals and can be advantageously used for the production of large quantities of the desired peptide. More particularly, the invention concerns the engineering of a transgenic animal having an artificial gene, which is controlled by globin locus control region (LCR) and which encodes a fusion protein, in which the desired peptide is joined via a cleavable peptide linker to a globin polypeptide. The erythrocytes of the transgenic animal express the fusion globin which is incorporated into hemoglobin produced by the host cell. The desired peptide can be obtained from a hemolysate of the red cells of the transgenic animals by enzymatic or chemical cleavage at the linker.Type: GrantFiled: June 7, 1994Date of Patent: May 6, 1997Assignee: DNX BioTherapeuticsInventors: Ramesh Kumar, Ajay Sharma, Anastasia M. Khoury-Christianson
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Patent number: 5618791Abstract: The invention is a class of memory enhancing peptides having the general formula:R.sub.1 --Asn--X.sub.1 --X.sub.2 --X.sub.3 --R.sub.2whereR.sub.1 =pyroglutamyl, acetyl, H, peptidylX.sub.1 =Ala, Ile, Leu, Tyr, Phe, Val, Trp, Cyt, HmeX.sub.2 =Pro, Leu, Ile, Val provided that when X.sub.2 is Pro, X.sub.1 is not Cyt or Ala;X.sub.3 =ArgR.sub.2 =OH, NH.sub.2, OR.sub.3, glycylandR.sub.3 =C.sub.1-6 alkyl.Type: GrantFiled: August 6, 1993Date of Patent: April 8, 1997Assignee: Shanghai Institute of BiochemistryInventor: Yu-Cang Du
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Patent number: 5596078Abstract: A process for preparing and purifying cyclic peptides having disulfide moieties in a single processing operation which simplifies synthesis and reduces production costs, yet produces high, quality yield. Higher yields are obtained by isolating the desired cyclic compound through direct ion exchange chromatography as an integral part of the single process. The improved process is particularly useful for the preparation of vasopressin and oxytocin and their respective derivatives and analogs.Type: GrantFiled: April 18, 1995Date of Patent: January 21, 1997Assignee: Ferring ABInventors: Lars H. H. Andersson, Jan-Ake Skoldback
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Patent number: 5500413Abstract: A process for the manufacture of 1-deamino-8-D-arginine vasopressin (DDAVP) comprising, condensing a preparation of Mpa(R.sup.1)-Tyr-Phe-Gln-Asn-Cys(R.sup.2)-Pro-OH (SEQ ID NO: 1), where R.sup.1 and R.sup.2 are sulfhydryl-protecting groups, with the dipeptide (R.sup.3)-D-Arg(HCl)-Gly-NH.sub.2, where R.sup.3 is an acid-sensitive amino-protecting group, to form Mpa(R.sup.1)-Tyr-Phe-Gln-Asn-Cys(R.sup.2)-Pro-D-Arg(HCl)-Gly-NH.sub.2 (SEQ ID NO: 1), which is oxidized with iodine in a protic solvent. The reaction mixture containing the oxidized product can be purified by ion exchange chromatography on a cation exchange resin equilibrated with acid. Also disclosed is high-purity DDAVP obtained by this process and its use for treating diurea.Type: GrantFiled: June 29, 1993Date of Patent: March 19, 1996Assignee: Ferring ABInventors: Krister Larsson, Thomas Mellbrand, Birgitta Mornstam, Jan Roschester, Jan-Ake Skoldback
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Patent number: 5486596Abstract: Analogues of 8-D-homoarginine vasopressin were prepared with the general formula ##STR1## where X is L-O-methyltyrosine, L-p-ethylphenylalanine, D-p-ethylphenylalanine, L-p-methylphenylalanine or D-methylphenylalanine and R is cysteine or .beta.-mercaptopropionic acid. These vasopressin analogues exhibited an increased affinity to uterus receptors for oxytocin, where they acted as ocytocin antagonists. Moreover, the analogues of deamino vasopressin have a significantly reduced antidiuretic activity in comparison with [8-D-arginine]vasopressin.Type: GrantFiled: August 25, 1993Date of Patent: January 23, 1996Assignee: Ceskoslovenska akademie vedInventors: Zdenko Prochazka, Ivo Blaha, Miroslava Zertova, Jirina Slaninova, Jiri Velek, Jana Skopkova, Michal Lebl, Tomislav Barth, Lenka Maletinska, Hans Vilhardt
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Patent number: 5482931Abstract: Disclosed is a stabilized aqueous composition for administration to a patient comprising a biologically active peptide, a buffer, a quaternary amine-type preservative or disinfectant, and an osmotic pressure-controlling agent, which composition can be stored and used at room temperature. The buffer stabilizes the pH of the composition between about 4 and 6. The preferred buffer contains citrate and/or phosphate, and the preferred preservative or disinfectant is benzalkonium chloride. The composition protects the peptide contained therein from adhering to container surfaces, particularly in containers made of polymeric materials.Type: GrantFiled: June 29, 1993Date of Patent: January 9, 1996Assignee: Ferring ABInventors: Alan Harris, Birgitta Tennhammar-Ekman
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Patent number: 5470948Abstract: This invention relates to new vasopressin and vasotocin derivatives of general formula I ##STR1## in which A stands for the radical Mca (=3-mercapto-3,3-cyclpentamethylenepropionyl radical) or for the radical Mpa (=3-mercaptopropionyl radical),B stands for the amino acid radicals D-Tyr, D-Tyr(Et), D-Phe, Tyr(OMe), D-lle, D-Trp, or the radical of a hydrophobic D-amino acid, andC stands for phenylalanine (Phe; vasopressin derivatives) or isoleucine (lle; vasotocin derivatives).They have a high activity toward the oxytocin and the V-vasopressin receptors and can be used for the production of pharmaceutical agents.Type: GrantFiled: December 20, 1993Date of Patent: November 28, 1995Assignee: Schering AktiengesellschaftInventors: Falk Fahrenholz, Walter Elger, Marianne Fahnrich, Kryzsztof Chwalisz
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Patent number: 5459077Abstract: Disclosed are methods for modelling the three-dimensional structure (tertiary structure) of a ligand having one or more active sites employing a charge-transfer interaction. Also disclosed is a model for Angiotensin II derived from such method.Type: GrantFiled: March 5, 1993Date of Patent: October 17, 1995Assignee: PepMetics, Inc.Inventors: Graham J. Moore, John M. Matsoukas
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Patent number: 5459236Abstract: The present invention relates to new vasotocin derivatives with prolonged activity compared to previous vasotocin derivatives. In particular, the vasotocin derivatives of the present invention are of the formula (I) ##STR1## wherein Hmp is a 2-hydroxy-3-mercaptopropionic acid residue, ##STR2## Z is Phe or Tyr, Y is Hgn or Hci, X is a residue of the formula ##STR3## wherein Q is H, alanyl or L-2-aminobutyryl and n is 1, 2 or 3. The present invention also relates to pharmaceutical compositions containing these vasotocin derivatives.Type: GrantFiled: September 23, 1992Date of Patent: October 17, 1995Assignee: Ferring ABInventors: Carl-Johan Aurell, Per Melin, Anders Nilsson, Jerzy Trojnar
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Patent number: 5434132Abstract: Oligopeptides have been found that antagonist cell proliferation stimulated not only by bombesin-like peptides but also other neuropeptides e.g. vasopressin and bradykinin. These oligopeptides block a broad family of mitogenic neuropeptides that bind to receptors that use the inositol signalling pathway and one such receptor has been identified. These oligopeptides are of particular interest in relation to small cell lung cancer.Type: GrantFiled: November 2, 1993Date of Patent: July 18, 1995Assignee: Imperial Cancer Research Technology, Ltd.Inventors: Enrique Rozengurt, Penella Woll
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Patent number: 5373089Abstract: A novel compound is disclosed for inhibiting the effects of oxytocin in a female mammal. As an analog of oxytocin the compound is named [(S)PMP.sup.1,D-Trp.sup.2,Pen.sup.6,Arg.sup.8 ] oxytocin. This compound can be administered to pregnant women to arrest premature labor while avoiding unwanted side effects due to antagonism of the antidiuretic hormone, vasopressin.Type: GrantFiled: April 26, 1993Date of Patent: December 13, 1994Assignee: Northwestern UniversityInventors: George Flouret, Laird Wilson
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Patent number: 5364851Abstract: Electrochemical methods, preferably the Kolbe coupling reaction, are utilized to create intramolecularly bridged peptides, segments or peptide isosteres which are conformationally restricted and preferably, biologically active. Preferably, the peptide analogues contain methylene groups bridging particular amino acid side chains. Analogues of a variety of peptide hormones, including insulin, insulin-like growth factors, somatostatin, melanocyte stimulating hormone, and the like are prepared by the above methods. Such peptides are useful as agonists or antagonists for treatment of diseases associated with deficiency of the hormone or dysregulation of hormone activity, as well as for mechanistic studies to understand the interactions between peptide hormones and cells.Type: GrantFiled: June 14, 1991Date of Patent: November 15, 1994Assignee: International Synthecon, LLCInventor: Alvin D. Joran
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Patent number: 5349050Abstract: Disclosed is a novel peptide having one of the formulae: ##STR1## (A and B are the amino acids: wherein if A is D- or L-Pro, B is Hat or Cit;if A is D-Pro, B is D-Arg, andif B is D- or L-Arg, A is Sat, Pip, Aze or Arg)Asn-A-L- (D-)Pro-Arg- (Gly)n (A is Set, Thr or Ala, n is 1 or 0)A-Ser-Pip-Arg (A is Pro-Asn-, Asn- or Pro-) ##STR2## (A is cyclopentylcarbonyl, Pro or pGlu; B is Gly or .beta.-Ala, W is a hydrogen atom or a group having the formula:or a peptide having the formula: ##STR3## wherein A and B have the same meanings as mentioned above, respectively pGlu-Asn-Ser-A-B-(Gly)n (A is Aze, D- or L-Pro, Pip or Sat, B is D- or L-Arg, Cit, Hat, Lys or Orn, n is 1 or 0) andPro- (Ash)m-Set-L- (D-)Pro-Arg- (Gly) n (m and n are independently 0 or 1)their functional derivatives, and pharmaceutically acceptable salts thereof.Type: GrantFiled: February 18, 1992Date of Patent: September 20, 1994Assignees: Nippon Chemiphar Co., Ltd., Fujirebio Kabushiki KaishaInventors: Mitsuo Masaki, Masaki Uehara, Kenji Hirate, Yoshikazu Isowa, Yoshiaki Sato, Yoshiharu Nakashima
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Patent number: 5225528Abstract: Disclosed are cyclic hexapeptides of the formula: ##STR1## These compounds are antagonists of oxytocin and are useful in the treatment of preterm labor and dysmenorrhea, and for stoppage of labor prepatory to Caesarian delivery. Also disclosed are pharmaceutical compositions containing the compounds of formula I and methods of preparing these compounds.Type: GrantFiled: December 17, 1990Date of Patent: July 6, 1993Assignee: Merck & Co., Inc.Inventors: Mark G. Bock, Roger M. Freidinger, Roger D. Tung, Daniel F. Veber, Peter D. Williams
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Patent number: 5180712Abstract: A peptide having one of the following formulae (I), (II), (III) and (IV): ##STR1## or its functional derivatives, and a pharmaceutically acceptable salt thereof are disclosed. These peptides have a nootropic effect and are effective as antidementia agents.Type: GrantFiled: August 14, 1989Date of Patent: January 19, 1993Assignees: Fujirebio Kabushiki Kaisha, Nippon Chemiphar Co., Ltd.Inventors: Yoshikazu Isowa, Yoshiaki Sato, Yoshiharu Nakashima, Mitsuo Masaki, Norihisa Miyake, Masaki Uehara, Kenji Hirate
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Patent number: 5095003Abstract: Cyclic peptides are produced by the controlled aerobic fermentation of Streptomyces silvensis, ATTCC No. 53525 or ATCC No. 53526. These compounds are antagonists of oxytocin and are useful in the treatment of preterm labor and vasopressin and are thus useful in the treatment and prevention of disease states wherein vasopressin may be involved, for example congestive heart failure, hypertension, edema and hyponatremia.Type: GrantFiled: October 23, 1990Date of Patent: March 10, 1992Assignee: Merck & Co., Inc.Inventors: Michael A. Goetz, Lawrence R. Koupal, Cheryl D. Schwartz, Jerrold M. Liesch, Otto D. Hensens, Paul S. Anderson, Roger Freidinger, Douglas J. Pettibone
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Patent number: 5070187Abstract: A unique class of vasopressin analogue antagonists is provided which have the pharmacological property in-vivo to antagonize pressor (V.sub.1) and/or antidiuretic (V.sub.2) activities. The chemical modifications to the vasopressin 9 member chain sequence at the no. 1, 2, and 4 positions yield a class of potent analogue antagonists which may be employed therapeutically to treat hypertension, congestive heart failure, various edematous situations, and a variety of symptoms due to inappropriate vasopressin secretion.Type: GrantFiled: November 3, 1989Date of Patent: December 3, 1991Assignee: Trustees of Boston UniversityInventors: Haralambos Gavras, Bernard Lammek
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Patent number: 5055448Abstract: Compounds acting as antagonists of the antidiuretic/and or vasopressor activity of arginine vasopressin are those of the formulaA--CH.sub.2 CO--D--Tyr(R)--Phe--Y--Asn--T--U--Z--Qwherein A is a-adamantyl, cyclohexyl, cyclopentyl, 1-mercaptocyclohexyl, 1-mercaptocyclopentyl, 1-ethyl-1-mercaptopropyl, yclohexylmethyl, cyclopentylmethyl, methyl, isopropyl, tert-butyl or phenyl; R is alkyl of 1-4 carbon atoms; Y is Val, Ile, Thr, Ala, Lys, Cha, Nva, Met, Nle, Orn, Ser, Asn, Gln, Phe, Tyr, Gly, Abu or Leu; T is Pen, Abu, Orn, Oys, Arg, Ala, Cha or Thr; U is Pro, Arg, Lys or Orn or a single bond; Z is (d-or L-) Arg, Orn or Lys and Q is Gly(NH.sub.2), Arg (NH.sub.2), Orn(NH.sub.2), Lys (NH.sub.2), (D- or L-)Ala(NH.sub.2), Ser(NH.sub.2), Val(NH.sub.2), Phe(NH.sub.2), Ile(NH.sub.2), Thr(NH.sub.2), Pro(NH.sub.2), Tyr(NH.sub.2), NH.sub.2, OH, NHR, NGbzl, NH(CH.sub.2).sub.p NH.sub.2 or NH(CH.sub.2).sub.p OH, wherein R is as above and p is an integer from 2 to 6. Compounds wherein T is Cys have similar activity.Type: GrantFiled: June 25, 1987Date of Patent: October 8, 1991Assignees: Medical College of Ohio, Trustees of Columbia UniversityInventors: Maurice Manning, Wilbur H. Sawyer
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Patent number: 5051526Abstract: Novel benzhydrylamine derivatives represented by general formulas (I) and (II) are useful as reaction reagents for the solid-phase synthesis of polypeptide amides making use of 9-fluorenylmethyloxycarbonyl group ("Fmoc"). An intermediate for these derivatives represented by the general formula (III) is also disclosed: ##STR1## where R.sub.1 and R.sub.2 each independently represents a C.sub.1-3 lower alkyl; n is an integer of 1-4; l is 1 or 2; and m is 1 or 2.Type: GrantFiled: May 24, 1990Date of Patent: September 24, 1991Assignee: Chugai Seiyaku Kabushiki KaishaInventors: Susumu Funakoshi, Eigoro Murayama
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Patent number: 4996194Abstract: Composition for the prevention and treatment of autoimmune diseases are provided which comprise as an active ingredient membrane material shed from autoimmune T lymphocytes, or activated T lymphocytes which are treated by a pressure application and releases process. There is also provided processes for obtaining such active materials and for preparing pharmaceutical compositions containing them.Type: GrantFiled: September 23, 1986Date of Patent: February 26, 1991Assignee: Yeda Research and Development Co. Ltd.Inventors: Irun R. Cohen, Meir Shinitzky
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Patent number: 4876243Abstract: Vasopressin-like peptide whose structures have been modified by an alpha-methyl amino acid at the 4 or 7 position are set forth. These compounds have potent vasopressin antagonist activities.Type: GrantFiled: August 27, 1987Date of Patent: October 24, 1989Assignee: Smithkline Beckman CorporationInventors: Garland R. Marshall, Michael L. Moore
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Patent number: 4829051Abstract: Novel analogs of biologically active vasopressin and its synthetic analogs having improved activity are represented by the structural formula: ##STR1## wherein A hydrogen; is hydroxy or lower alkoxy, especially methoxy, B is the peptide residue of phenylalanine (Phe) or isoleucine, Gln represents the peptide residue of glutamine Asn represents the peptide residue of asparagine, E represents the peptide residue of proline (Pro), 4-thioproline (4-thioPro) or 3,4-dehydroproline (3,4-dehydroPro), FNH represents an N-substituted peptide residue of L- or D-lysine (Lys) L or D homolysine (h Lys) or L- or D-orntihine (Orn), X represents a side peptide chain consisting of one or more of Gly, L-Ala, L-Val, L-Leu, or L-Phe, Gly represents the peptide residue of glycine, and G is disulfide (--S--S--) or thioether (--CH.sub.2 S-- or --SCH.sub.2 --).Type: GrantFiled: November 16, 1984Date of Patent: May 9, 1989Assignee: Vega Laboratories, Inc.Inventors: Joseph H. Cort, Alan J. Fischman
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Patent number: 4826813Abstract: Vasopressin antagonists which have a 4'-methyl-.beta.-mercapto-.beta.,.beta.-cyclopentamethylenepropionic acid group have vasopressin antagonist activity without substantial agonist activity. A species of the invention is [1-(4'-methyl-.beta.-mercapto-.beta.,.beta.-cyclopentamethylenepropionic acid)-2-(O-ethyl)-D-tyrosine-4-valine-8-arginine]vasopressin.Type: GrantFiled: May 21, 1987Date of Patent: May 2, 1989Assignee: SmithKline Beckman CorporationInventors: William F. Huffman, Nelson C. F. Yim
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Patent number: 4786631Abstract: The invention concerns novel AVP-binding peptides having the formula:Thr-Met-X-Val-Leu-Thr-Gly-Ser-Pro-Bwherein X is selected from the group consisting of Lys, Arg, and Asp; B is OH, NH.sub.2, NHAlk, wherein Alk is lower alkyl of 1-4 carbons, inclusive. These peptides block AVP function, and, therefore, are useful in many areas among which is the control of hypertension.Type: GrantFiled: March 18, 1988Date of Patent: November 22, 1988Assignee: University of FloridaInventors: Howard M. Johnson, Barbara A. Torres
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Patent number: 4772586Abstract: Compounds acting as antagonists of the antidiuretic/and or vasopressor activity of arginine vasopressin are those of the formula ##STR1## wherein n is 4 or 5; X is (D- or L-)Tyr(R), D-Phe, D-Val, D-Leu, D-Ile, D-Nva, D-Nle, D-Cha, D-Abu, D-Thr, D-Asn, D-Met or D-Gln; Y is Val, Ile, Thr, Ala, Lys, Cha, Nva, Met, Nle, Orn, Ser, Asn, Gln, Phe, Tyr, Gly, Abu or Leu; Z is (D- or L-) Arg, Orn or Lys; Q is Arg(NH.sub.2), Ser(NH.sub.2), (D- or L-)Ala(NH.sub.2), Gly, OH or NH.sub.2 and R is methyl, ethyl, propyl or butyl; provided that, when Y is Gln or Val, R may also be H. Further compounds are those wherein Q is NH(CH.sub.2).sub.3-6 NH.sub.2, NH(CH.sub.2).sub.2-6 OH, NHalk or NHbzl, provided that when Y is Val, Q is other than NHalk or NHbzl. Other compounds are those wherein Q is M-P, wherein M is Gly, (D- or L-)beta-Ala, (D- or L-)Ala, Sar, Orn, Val, Phe, Ile, Thr, Pro, Tyr, Arg or Ser and P is NHalk, NHbzl, NH(CH.sub.2).sub.2-6 OH, NH(CH.sub.2).sub.2-6 NH.sub.Type: GrantFiled: December 30, 1986Date of Patent: September 20, 1988Assignees: Medical College of Ohio, Trustees of Columbia UniversityInventors: Maurice Manning, Wilbur H. Sawyer
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Patent number: 4766108Abstract: Vasopressin antagonists which have a dipeptide tail composed of a neutral and basic amino acid unit demonstrate potent V.sub.1 and V.sub.2 -antagonist activity. Two species of this invention, which are prepared by solid phase peptide synthesis, are [1-(.beta.-mercapto-.beta.,.beta.-cyclopentamethylene propionic acid)-2-(O-ethyl)-D-tyrosine-4-valine-7-tyrosine-8-arginine-9-desglycine]- vasopressin and [1-(.beta.-mercapto-.beta.,.beta.-cyclopentamethylenepropionic acid)-2-(O-ethyl)-D-tyrosine-4-valine-7-arginine-8-tyrosine-9-desglycine]- vasopressin.Type: GrantFiled: December 4, 1986Date of Patent: August 23, 1988Assignee: Smith Kline Beckman CorporationInventor: Fadia E. Ali
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Patent number: 4760052Abstract: New compounds which have potent V.sub.2 -vasopressin antagonistic activity are prepared by a 1,6-cyclization using peptide bond formation. The structures of the compounds are characterized by a Pas.sup.1,6 or Tas.sup.1,6 cyclized unit. Also a chiral synthesis of the optically pure Pas intermediates is described.Type: GrantFiled: April 28, 1987Date of Patent: July 26, 1988Assignee: Smithkline Beckman CorporationInventors: James F. Callahan, William F. Huffman, Kenneth A. Newlander, Nelson C. F. Yim
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Patent number: 4742154Abstract: Peptides whose structures resemble those of vasopressin antagonists but have both a carboxamido amino acid unit and a basic amino acid unit in the side chain have good antagonist activity. A species of the invention is [1-(.beta.-mercapto-.beta.,.beta.-cyclopentamethylenepropionic acid)-2-(O-ethyl)-D-tyrosine-4-valine-8-glutamine-9-desglycine]-vasopressi n.Type: GrantFiled: October 23, 1985Date of Patent: May 3, 1988Assignee: Smithkline Beckman CorporationInventors: William M. Bryan, William F. Huffman, Michael L. Moore
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Patent number: 4724229Abstract: Vasopressin antagonists which have a tripeptide side chain comprised of three basic amino acids, such as arginine, lysine or ornithine, demonstrate potent V.sub.1 and V.sub.2 -antagonist activity. A species of the invention, which is prepared by conventional peptide sequencing, is [1-(.beta.-mercapto-.beta.,.beta.-cyclopentamethylenepropionic acid)-2-(O-ethyl)-D-tyrosine-4-valine-7-arginine-8-arginine-9-arginine]-va sopression.Type: GrantFiled: September 30, 1986Date of Patent: February 9, 1988Assignee: Smithkline Beckman CorporationInventor: Fadia E. Ali
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Patent number: 4717715Abstract: Vasopressin antagonists which have a dipeptide side chain comprised of two basic amino acids demonstrate potent V.sub.1 and V.sub.2 -antagonist activity. A species of the invention, which is prepared by conventional peptide sequencing, is [1-(.beta.-mercapto-.beta.,.beta.-cyclopentamethylene propionic acid)-2-(O-ethyl)-D-tyrosine-4-valine-7-arginine-8-arginine-9-desglycine]- vasopressin.Type: GrantFiled: June 23, 1986Date of Patent: January 5, 1988Assignee: SmithKline Beckman CorporationInventor: Fadia E. Ali
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Patent number: 4714696Abstract: Compounds acting as antagonists of the antidiuretic/and or vasopressor activity of arginine vasopressin are those of the formula ##STR1## wherein n is 4 or 5; X is (D- or L-)Tyr(R), D-Phe, D-Val, D-Leu, D-Ile, D-Nva, D-Nle, D-Cha, D-Abu, D-Thr, D-Asn, D-Met or D-Gln; Y is Val, Ile, Thr, Ala, Lys, Cha, Nva, Met, Nle, Orn, Ser, Asn, Gln, Phe, Tyr, Gly, Abu or Leu; Z is (D- or L-) Arg, Orn or Lys; Q is Arg(NH.sub.2), Ser(NH.sub.2), (D- or L-)Ala(NH.sub.2), Gly, OH or NH.sub.2 and R is methyl, ethyl, propyl or butyl; provided that, when Y is Gln or Val, R may also be H.Type: GrantFiled: January 26, 1984Date of Patent: December 22, 1987Assignees: Medical College of Ohio, Trustees of Columbia UniversityInventors: Maurice Manning, Wilbur H. Sawyer
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Patent number: 4711877Abstract: Vasopressin derivatives having unexpected VSP site binding activity whose structures are characterized by a Mpr unit at position 1 and a Pen unit at position 6 are prepared by standard peptide synthetic methods also using an oxidative cyclization of a linear peptide dimercaptan. A representative species is [1-.beta.-mercaptopropionic acid-2-(O-ethyl)-D-tyrosine-4-valine-6-penicillamine-8-arginine]vasopressi n.Type: GrantFiled: September 18, 1985Date of Patent: December 8, 1987Assignee: SmithKline Beckman CorporationInventor: Michael L. Moore
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Patent number: 4709014Abstract: A peptide with C-terminal proline amide is produced by reacting in aqueous solution a protein substrate having C-terminal prolyl-leucine, prolyl-isoleucine, prolyl-valine, or prolyl-phenylalanine with carboxypeptidase Y in the presence of ammonia. The ammonia is preferably generated by the aqueous reaction of an ammonium salt and an alkali. An example of the peptide product is human calcitonin.Type: GrantFiled: April 1, 1986Date of Patent: November 24, 1987Assignee: Sankyo Company LimitedInventor: Hidetsune Tamaoki
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Patent number: 4687758Abstract: Peptides having vasopressin antagonist activity are prepared by a peptide synthesizer to insert a N-methylarginine at the 7-position of the structure. An example of this series of compounds is [1-(.beta.-mercapto-.beta.,.beta.-cyclopentamethylenepropionic acid)-2-(O-ethyl-D-tyrosine)-4-valine-7-desproline-8-N-methylarginine-9-de sglycine]-vasopressin.Type: GrantFiled: November 19, 1985Date of Patent: August 18, 1987Assignee: SmithKline Beckman CorporationInventors: Fadia E. Ali, William F. Huffman, Garland R. Marshall, Michael L. Moore
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Patent number: 4658015Abstract: Certain vasopressin-like peptides, which have an acyclic unit at position 1 and which have an .omega.-amino- or guanidinoalkyl substituent attached to the cysteine in the 6-position of the ring, have V.sub.1 -vasopressin and oxytocin antagonist activity. A species of this series of new compounds is [1-desaminopenicillamine-2-(O-ethyl-D-tyrosine)-8-(1,4-diaminobutane)-9-de sglycinamide]-vasopressin.Type: GrantFiled: April 16, 1986Date of Patent: April 14, 1987Assignee: SmithKline Beckman CorporationInventors: James F. Callahan, William F. Huffman, Michael L. Moore, Nelson C. Yim
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Patent number: 4656248Abstract: The preparation of [1-(.beta.-mercapto-.beta.,.beta.-cyclopentamethylenepropionic acid)-2-(O-ethyl-D-tyrosine)-4-valine-8-arginine-9-desglycine]vasopressin by oxidation of the corresponding dimercaptan is improved by using a copper II salt.Type: GrantFiled: April 23, 1985Date of Patent: April 7, 1987Assignee: SmithKline Beckman CorporationInventors: Suresh M. Kalbag, Paul J. Voelker
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Patent number: 4649130Abstract: Compounds acting as antagonists of the antidiuretic/and or vasopressor activity of arginine vasopressin are those of the formula ##STR1## wherein n is 4 or 5; X is (D- or L-)Tyr(R), D-Phe, D-Val, D-Leu, D-lle, D-Nva, D-Nle, D-Cha, D-Abu, D-Thr, D-Asn, D-Met or D-Gln; Y is Val, lle, Thr, Ala, Lys, Cha, Nva, Met, Nle, Orn, Ser, Asn, Gln, Phe, Tyr, Gly, Abu or Leu; Z is (D- or L-) Arg, Orn or Lys; Q is Arg(NH.sub.2), Ser(NH.sub.2), (D- or L-)Ala(NH.sub.2), Gly, OH or NH.sub.2 and R is methyl, ethyl, propyl or butyl; provided that, when Y is Gln or Val, R may also be H. Further compounds are those wherein Q is Orn(NH.sub.2), NHCH.sub.2 CH.sub.2 NH.sub.2, Val(NH.sub.2), Phe(NH.sub.2), lle(NH.sub.2), Thr(NH.sub.2), Pro(NH.sub.2) or Tyr(NH.sub.2).Type: GrantFiled: January 23, 1985Date of Patent: March 10, 1987Assignees: Medical College of Ohio, The Trustees of Columbia UniversityInventors: Maurice Manning, Wilbur H. Sawyer
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Patent number: 4624943Abstract: Certain new vasopressin-like peptides which have structures characterized by having a bisaminoalkylbenzene present in the vasopressin tail at the 7- or 8-position retain vasopressin antagonist activity. A species of the invention is [1-.beta.-mercapto-.beta.,.beta.-cyclopentamethylenepropionic acid)-2-(O-ethyl)-D-tyrosine-4-valine-8-1',4'-bis(aminomethyl)benzene-9-de sglycinamide]-vasopressin.Type: GrantFiled: March 20, 1985Date of Patent: November 25, 1986Assignee: SmithKline Beckman CorporationInventors: James F. Callahan, Michael L. Moore, Nelson C. Yim
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Patent number: 4604378Abstract: Certain vasopressin-like peptides, which have an acyclic unit at position 1 and which have an .omega.-amino- or guanidinoalkyl substituent attached to the cysteine in the 6-position of the ring, have V.sub.1 -vasopressin and oxytocin antagonist activity. A species of this series of new compounds is [1-desaminopenicillamine-2-(O-ethyl-D-tyrosine)-8-(1,4-diaminobutane)-9-de sglycinamide]-vasopressin.Type: GrantFiled: November 21, 1984Date of Patent: August 5, 1986Assignee: SmithKline Beckman CorporationInventors: James F. Callahan, William F. Huffman, Michael L. Moore, Nelson C. Yim
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Patent number: 4599324Abstract: Vasopressin derivatives having V.sub.1 and oxytocin antagonist activity whose structures are characterized by a Mpa unit at position 1 and a des-Pro unit at position 7 are prepared by standard peptide synthetic methods also using an oxidative cyclization of a dimercaptan. Representative species are [1-deaminopenicillamine-2-(O-methyl)-tyrosine-7-desproline-8-arginine-9-de sglycine]vasopressin or [1-.beta.-mercaptopropionic acid-2-D-(O-ethyl)tyrosine-3-isoleucine-4-threonine-7-desproline-8-arginin e-9-desglycine]-vasopressin.Type: GrantFiled: November 21, 1984Date of Patent: July 8, 1986Assignee: SmithKline Beckman CorporationInventors: Fadia E. Ali, William F. Huffman
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Patent number: 4596819Abstract: Modified oxytocin-vasopressin di- or tri-peptides wherein an amide group is replaced by a group selected from ##STR1## --CH.sub.2 NH--, --CH.sub.2 O--, --CH.sub.2 S--, --CH.sub.2 SO--, --CH.sub.2 SO.sub.2 --, --CH.sub.2 CH.sub.2 -- and --CH.dbd.CH-- are described as well as methods for their manufacture, pharmaceutical compositions and methods for treatment, especially of cognitive disorders, depression, morphine dependency and parkinsonism by adjunctive therapy.Type: GrantFiled: January 23, 1984Date of Patent: June 24, 1986Assignee: Warner-Lambert CompanyInventors: Ernest D. Nicolaides, Francis J. Tinney, James S. Kaltenbronn, Dana E. DeJohn, Elizabeth A. Lunney, W. Howard Roark, Joseph T. Repine