Immunological Separation Or Affinity Chromatography Patents (Class 530/413)
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Patent number: 8367810Abstract: Disclosed are substantially purified Plasmodium sporozoites and preparations of Plasmodium sporozoites substantially separated from attendant non-sporozoite material, where the preparations of Plasmodium sporozoites have increasing levels of purity. Vaccines and pharmaceutical compositions comprising purified Plasmodium sporozoites are likewise provided. Methods of purifying preparations of Plasmodium sporozoites are also provided.Type: GrantFiled: August 27, 2010Date of Patent: February 5, 2013Assignee: Sanaria Inc.Inventors: B. Kim Lee Sim, Minglin Li, Richard E. Stafford, Stephen L. Hoffman
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Patent number: 8357301Abstract: Herein is reported a method for determining whether a re-useable chromatography column packing, which is used at least for the second time in a purification step of a purification of a polypeptide, has reduced separation efficacy in said purification step of said purification of said polypeptide, comprising the following steps: a) identifying and determining the experimental data of an inert change of at least one physicochemical parameter of a mobile phase passing through said re-useable chromatography column packing, b) determining the parameters of a function of formula I by fitting the experimental data of the inert change of the physicochemical parameter of the at least second use, c) determining the difference between the experimental data of the inert change of the physicochemical parameter of the at least second use and the function of formula I with the parameters determined in step b), d) calculating the difference between the maximum value and the minimum value of the difference determined in stepType: GrantFiled: June 22, 2010Date of Patent: January 22, 2013Assignee: Hoffmann-La Roche, Inc.Inventors: Anton Belousov, Thomas Dams, Benjamin Gerwat
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Patent number: 8357376Abstract: The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.Type: GrantFiled: January 5, 2010Date of Patent: January 22, 2013Assignee: Memimmune, LLCInventors: Jonathan Liu, Richard Schwartz, Mark Thompson, Luis Jorge Camilo Maranga, Simon Sheng-Tsiung Hsu, Mridul Ghosh, Ajit Subramanian
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Publication number: 20130018172Abstract: Compositions comprising certain tripeptides which are capable of binding a metal in a square planar orientation, a square pyramidal orientation, or both, are disclosed in processes for modulating tagged peptides or proteins. Such compositions and processes may be used for site-specific chiral inversion of amino acids, site-specific peptide cleavage, and protein purification, among other uses.Type: ApplicationFiled: January 13, 2012Publication date: January 17, 2013Applicants: Echogen, Inc., University of KansasInventors: Jennifer Ann Stowell Laurence, Mary Elizabeth Krause, Timothy A. Jackson, George Laurence
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Patent number: 8354510Abstract: The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.Type: GrantFiled: September 29, 2009Date of Patent: January 15, 2013Assignee: GE Healthcare Bio-Sciences ABInventors: Sophia Hober, Hans J. Johansson
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Publication number: 20130012597Abstract: The present invention provides a UCST-type thermoresponsive polymer compound which responds to a temperature under physiological conditions and has biofunctionality, and various uses thereof as a thermoresponsive material. Specifically, the invention provides a thermoresponsive material containing, as an active ingredient, a polymer compound represented by Formula (I) below or an addition salt thereof, the thermoresponsive material having an upper critical solution temperature in the range of 5 to 50° C. in an aqueous solution with a salt concentration of at least 1 mM and a pH in the range of 3 to 10.5: wherein m represents an integer of 10 or more; n represents a number satisfying 0.4?n?1; R1 represents hydrogen or succinyl; and R2 represents carbamoyl.Type: ApplicationFiled: March 22, 2011Publication date: January 10, 2013Applicant: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATIONInventors: Naoko Shimada, Atsushi Maruyama
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Patent number: 8350013Abstract: The invention relates to methods for isolating a product and/or reducing turbidity and/or impurities from a load fluid comprising the product and one or more impurities by passing the load fluid through a medium, followed by at least one wash solution comprising arginine, and collecting the product using an elution solution. The invention further relates to a product prepared using a method as described herein.Type: GrantFiled: September 7, 2007Date of Patent: January 8, 2013Assignee: Wyeth LLCInventor: Shujun Sun
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Publication number: 20120328628Abstract: The present invention provides methods for generating antibodies to specific conformations of proteins. The conformation specific antibodies of the invention can be put to a variety of uses including diagnosis and treatment of diseases and for screening for compounds that induce conformational changes in proteins upon binding.Type: ApplicationFiled: July 6, 2007Publication date: December 27, 2012Applicant: The Regents of the University of CaliforniaInventors: James A. Wells, Junjun Gao
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Patent number: 8338577Abstract: The present invention provides methods of quantifying protein leakage from a protein based affinity chromatography media (e.g., protein A, protein G and protein L based affinity chromatography media), where such a protein is used for isolating and/or removing a molecule which binds the protein (e.g., an immunoglobulin).Type: GrantFiled: September 15, 2009Date of Patent: December 25, 2012Assignee: EMD Millipore CorporationInventor: Nanying Bian
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Publication number: 20120322983Abstract: The present invention provides a method for purifying a protein to remove impurities from a mixture liquid containing a desired protein and the impurities, comprising performing filtration using a porous membrane having a graft chain on a pore surface and an anion-exchange group fixed to the graft chain.Type: ApplicationFiled: August 13, 2012Publication date: December 20, 2012Applicant: ASAHI KASEI CHEMICALS CORPORATIONInventors: Hironobu SHIRATAKI, Naoyuki SHINOHARA
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Patent number: 8329422Abstract: The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CELLSPOTTER® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.Type: GrantFiled: November 2, 2010Date of Patent: December 11, 2012Assignee: Veridex LLCInventors: Galla Chandra Rao, Christopher Larson, Madeline Repollet, Herman Rutner, Leon W. M. M. Terstappen, Shawn Mark O'Hara, Steven Gross
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Patent number: 8323634Abstract: Described herein are compositions and methods for treating, preventing and ameliorating diseases and conditions characterized by a lower than normal white blood cell count, such as leukopenia and neutropenia. The compositions and methods include recombinant human albumin-human granulocyte colony stimulating factor. Pharmaceutical formulations including the recombinant fusion protein, and methods of making such formulations are also described.Type: GrantFiled: January 15, 2010Date of Patent: December 4, 2012Assignee: Teva Pharmaceutical Industries Ltd.Inventors: Jason Benjamin Bock, Xia Luo
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Publication number: 20120289680Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.Type: ApplicationFiled: July 27, 2012Publication date: November 15, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: MARTIN HALL, STURE LARSSON, ANDREAS MURANYI, GUSTAV RODRIGO, JINYU ZOU, PER-MIKAEL ÅBERG
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Publication number: 20120276135Abstract: There exist in the art methods of detecting simple peptides. However, methods to determine the effective plasma concentration of mixtures of peptides as a group, rather than for individual peptides with a defined amino acid sequence, are complicated by the heterogeneity of the peptides to be detected. This application provides improved methods of detecting and assessing random sequence polymer (RSP) compositions, methods for the detection and quantitation of RSP compositions, means to determine and enrich a subset of peptides in an RSP composition based on the subset's interactions with certain capture polypeptides, and methods for administering RSP compositions to a subject in need thereof, wherein the dosage regimen and quantity may be determined or evaluated based on the above-mentioned methods for detection and quantitation.Type: ApplicationFiled: November 17, 2010Publication date: November 1, 2012Applicant: Ares Trading SAInventors: Eric H. Zanelli, Jeff Krieger, Joe Connolly, Kathryn H. Collins
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Publication number: 20120276623Abstract: Removal of genomic DNA from biological samples using cation exchange is described.Type: ApplicationFiled: April 28, 2011Publication date: November 1, 2012Applicant: Bio-Rad Laboratories, Inc.Inventor: Peter S. Gagnon
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Publication number: 20120269847Abstract: Bordetella pertussis p69 antigen is purified by immobilised metal affinity chromatography.Type: ApplicationFiled: May 10, 2010Publication date: October 25, 2012Applicant: NOVARTIS AGInventor: Christian A. Lease
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Patent number: 8293500Abstract: The present invention provides methods for identifying viral virulence factors and for identifying cellular polypeptides to which the viral polypeptides bind. The cellular polypeptide is useful as a therapeutic target or as a therapeutic agent for treating diseases and disorders, including immunological diseases or disorders.Type: GrantFiled: March 22, 2007Date of Patent: October 23, 2012Assignee: Viral Logic Systems Technology Corp.Inventors: Steven Wiley, Craig A Smith, Ajamete Kaykas
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Publication number: 20120264920Abstract: The invention is directed to a method for purifying a protein. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, inactivating viruses in the sample, and processing through at least one depth filter and ion-exchange membrane.Type: ApplicationFiled: October 11, 2011Publication date: October 18, 2012Applicant: ABBOTT LABORATORIESInventors: Chen Wang, Robert K. Hickman
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Publication number: 20120259094Abstract: The present invention relates generally to affinity ligands and chemical affinity ligand-matrix conjugates for use as chromatographic adsorbents and methods which utilise the adsorbents in the purification of proteins by affinity chromatography. The affinity ligand-matrix conjugates of the present invention comprise ligands of general formula (I): wherein m represents an integer from 0-2, n represents an integer from 0-6, p represents an integer from 0-4, R1 represents H or C1-3 alkyl, R2 is an optional substituent, and X is the position at which the ligand is immobilized, optionally via a linker.Type: ApplicationFiled: October 15, 2010Publication date: October 11, 2012Inventors: Milton TW Hearn, Anjali Makarand Bhagwat, William Roy Jackson, Simon John Mountford
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Patent number: 8283443Abstract: The present invention provides a method for purifying a protein, includes the step of: contacting a fusion protein of a first protein and a second protein with a bivalent cation-containing solution, the fusion protein being adsorbed to a silicon oxide-containing substance, the first protein being capable of binding to the silicon oxide-containing substance in a solution containing 0.1M sodium chloride. With this arrangement, it is possible to easily produce large quantity of proteins which are high in purity without sacrificing activity of the proteins.Type: GrantFiled: July 9, 2009Date of Patent: October 9, 2012Assignee: Hiroshima UniversityInventors: Akio Kuroda, Takeshi Ikeda
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Publication number: 20120252098Abstract: The invention provides methods and materials for using apatite chromatography supports to dissociate and remove contaminants that are complexed to biological products. The invention further provides materials and methods for dissociating aggregations of target biological molecules or improperly folded target molecules to improve purification of the target molecule.Type: ApplicationFiled: September 20, 2011Publication date: October 4, 2012Applicant: Bio-Rad Laboratories, Inc.Inventor: Peter S. Gagnon
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Patent number: 8258270Abstract: In an affinity-type purification, ligands dissociated from the stationary phase that would otherwise leach into the species being purified are captured by a second ligand that is also incorporated into the stationary phase, the second ligand exhibiting an affinity-type interaction with the dissociated first ligand with sufficient specificity to avoid the undesired retention by the second ligand of species from the liquid sample or source liquid other than the species sought to be purified in the affinity column.Type: GrantFiled: May 8, 2009Date of Patent: September 4, 2012Assignee: Bio-Rad Laboratories, Inc.Inventor: Mark A. Snyder
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Publication number: 20120214976Abstract: The invention provides a process for the purification recombinantly expressed, self-assembled VLP from the homogenate of a bacterial host, wherein the process can be scaled up to a commercial production scale in a cost effective manner. The process comprises a first chromatography using an anion exchange matrix, a second chromatography using hydroxyapatite and, optionally, a size exclusion chromatography. VLP preparations obtained by the process of the invention are essentially free of endotoxin contaminations.Type: ApplicationFiled: August 22, 2011Publication date: August 23, 2012Applicant: Cytos Biotechnology AGInventors: Susanne Richter, Simon Topell
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Publication number: 20120190124Abstract: The present invention relates to the use of a compound containing a moiety of formula (I) as a reagent for linking a compound of formula R1—H which comprises a first functional moiety of formula F1 to a second functional moiety of formula F2 wherein X, X?, Y, R1, F1 and F2 are as defined herein. The present invention also provides related processes and products. The present invention is useful for creating functional conjugate compounds, and specifically conjugates in which at least one of the constituent molecules carries a thiol group.Type: ApplicationFiled: August 9, 2010Publication date: July 26, 2012Inventors: Mark Smith, Stephen Caddick, James Baker
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Patent number: 8227599Abstract: A process for the separation of biological materials, such as dye-ligand affinity chromatography, wherein an adsorbent is used, which comprises a reaction product of certain reactive anthroquinone compounds and a substrate having a group capable of reaction with a reactive group in said reactive anthroquinone compounds to form a covalent bond.Type: GrantFiled: March 29, 2006Date of Patent: July 24, 2012Assignee: BASF SEInventors: Vadiraj Subbanna Ekkundi, Narayan S. Punekar, Kamalesh Pai Fondekar, Georg Roentgen, Shilpa S. Korde, Ajit B. Shinde, Masana Moorthy
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Patent number: 8222379Abstract: A method of separating a selected ionic component from a sample, comprises contacting the sample with an ionic adsorbent whose charge density is such that the component is bound selectively in the absence of added ionic component that competitively binds the adsorbent.Type: GrantFiled: September 5, 2003Date of Patent: July 17, 2012Assignee: EMD Millipore CorporationInventor: Robert John Noel
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Patent number: 8217153Abstract: Various system and method embodiments of the present invention are directed to separating target molecules from complex solutions by affinity column chromatography using organic-solvent-containing eluants. In one embodiment of the present invention, an eluant containing an organic-solvent is used, at a first pH, to remove non-target solutes and suspended entities from an affinity chromatography column. The pH of the eluant is then changed to a second pH, and the organic-solvent-containing eluant is used to elute target molecules from the affinity column chromatography.Type: GrantFiled: January 25, 2010Date of Patent: July 10, 2012Assignee: Amgen Inc.Inventor: Joe Xin Hua Zhou
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Patent number: 8217152Abstract: The invention relates to a process for the production of purified IL-18 binding protein (IL-18BP) from a fluid comprising affinity chromatography.Type: GrantFiled: June 8, 2006Date of Patent: July 10, 2012Assignee: Ares Trading S.A.Inventors: Claire Le Strat, Frederic Meuwly, Pierre Wenger, Pascal Valax, Gianni Baer, Henri Kornmann
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Patent number: 8211682Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: October 3, 2008Date of Patent: July 3, 2012Assignee: Natrix Separations Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Publication number: 20120165268Abstract: Biologically active tetrameric p53 proteins and p53 fusion proteins are provided. These proteins may be generated and refolded into tetrameric form using denatured proteins produced from E. coli. Therapeutic uses of p53 proteins and p53 fusion proteins are also provided.Type: ApplicationFiled: May 28, 2009Publication date: June 28, 2012Applicant: Proteom Tech, Inc.Inventors: Xinli Lin, Michelle Lafevre-Bernt
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Publication number: 20120165250Abstract: The invention provides a peptide triazole conjugate and derivatives thereof, and methods of its use. Further provided are an antibody to the peptide triazole conjugate, and a method of identifying an HIV-I entry inhibitor candidate.Type: ApplicationFiled: June 24, 2010Publication date: June 28, 2012Inventors: Irwin M. Chaiken, Umashankara Muddegowda, Karyn McFadden
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Patent number: 8206958Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: December 5, 2007Date of Patent: June 26, 2012Assignee: Natrix Separations Inc.Inventors: Ronald F. Childs, Tapan K. Dey, Carlos Filipe, Raja Ghosh, Marcus Y. Kim, Elena N. Komkova, Alicja M. Mika, Jinsheng Zhou
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Patent number: 8206982Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: October 14, 2008Date of Patent: June 26, 2012Assignee: Natrix Separations Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Publication number: 20120156757Abstract: The present invention relates, at least in part, to novel and improved chromatography matrices for separating or purifying immunogens, such as, for example, viruses and viral surface proteins, from one or more contaminants in a sample, where the matrix comprises a porous non-polysaccharide solid support comprising a negatively charged, multivalent ion exchange group directly attached to the solid support.Type: ApplicationFiled: December 13, 2011Publication date: June 21, 2012Applicant: Millipore CorporationInventors: Ganapathysubramanian Iyer, Joaquin A. Umana, Neil Soice
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Publication number: 20120149885Abstract: Large-scale downstream processing of secreted recombinant proteins is provided in a single device, wherein the contents of a plurality of bioreactors are combined simultaneous to their harvesting and purification resulting in significant savings of time and the cost of manufacturing.Type: ApplicationFiled: February 21, 2012Publication date: June 14, 2012Applicant: Therapeutic Proteins International LLCInventor: Sarfaraz K. Niazi
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Patent number: 8192971Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: September 16, 2008Date of Patent: June 5, 2012Assignee: Natrix Separations Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Patent number: 8187880Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: April 23, 2008Date of Patent: May 29, 2012Assignee: Natrix Separations, Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Publication number: 20120129150Abstract: The invention provides devices, test kits and methods for removing target agents from a sample. The device contains one or more porous matrices having pore sizes larger than 10 ?m, and a plurality of particles impregnated therein. The target agents attach the device and are removed from the sample.Type: ApplicationFiled: December 7, 2011Publication date: May 24, 2012Applicant: PATHOGEN REMOVAL AND DIAGNOSTIC TECHNOLOGIES INC.Inventor: Ruben G. Carbonell
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Publication number: 20120122125Abstract: The present disclosure is directed to reagents and methods of using the reagents to detect epitopes of Trypanosoma cruzi.Type: ApplicationFiled: January 19, 2012Publication date: May 17, 2012Applicant: ABBOTT LABORATORIESInventors: Susan E. Brophy, David J. Hawksworth, Dinesh O. Shah, Robert W. Siegel, Bryan C. Tieman, Bailin Tu, Joan D. Tyner, Robert N. Ziemann
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Publication number: 20120122076Abstract: The present invention relates to compositions and methods for the chromatographic purification of antibodies, such as monoclonal antibodies, employing improved simulated moving bed separation strategies and, in certain embodiments, Raman spectroscopy.Type: ApplicationFiled: September 16, 2011Publication date: May 17, 2012Applicant: Abbott LaboratoriesInventors: Siu-Man Lau, Diane Dong, Stephen Lu
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Patent number: 8173783Abstract: The present invention mainly relates to a process for isolation and purification of yolk antibodies from egg yolk of an anseriform bird by an adsorption chromatographic procedure using a water insoluble non-charged absorbent to accomplish a desired separation of yolk antibodies, and by a salting-out procedure that differentially precipitates the IgY antibodies. The present invention also relates to the yolk antibodies produced thereby and various uses of such yolk antibodies.Type: GrantFiled: April 21, 2006Date of Patent: May 8, 2012Assignee: Good Biotech CorporationInventor: Victor Chiou
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Publication number: 20120100173Abstract: The present invention relates to methods for preparing and using multichaperone-antigen complexes. The present invention uses HOP affinity molecules in affinity methods to isolate multichaperone (multi-HSP)-antigen complexes. Such complexes have use in therapy.Type: ApplicationFiled: April 2, 2010Publication date: April 26, 2012Applicant: Agenus Inc.Inventors: Kenneth P. Leclair, Andrew J. Tomlinson
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Patent number: 8163890Abstract: Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.Type: GrantFiled: April 25, 2006Date of Patent: April 24, 2012Assignees: Sandoz AG, Boehringer Ingelheim RCV GmbH & Co KGInventors: Alois Jungbauer, Rainer Hahn, Anne Tscheliessnig, Waltraud Kaar
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Publication number: 20120094937Abstract: Purified genes encoding a T cell surface antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this antigen are provided. Methods of using said reagents and diagnostic kits are also provided.Type: ApplicationFiled: December 20, 2011Publication date: April 19, 2012Applicant: Schering CorporationInventors: Daniel M. Gorman, Troy D. Randall, Albert Zlotnik
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Publication number: 20120093871Abstract: Modified protozoa parasites comprising simultaneous expression on its surface of at least two ariable surface proteins (VSP). The modified protozoa may also simultaneously express the complete repertoire of variable surface proteins. Protozoa show reduced expression of Dicer, RNA-dependant RNA-plymerase (RdRP) enzymes or both, wherein the RdRP gene and/or the Dicer gene has been silenced. The protozoan may be any protozoan showing an antigenic variation mechanism.Type: ApplicationFiled: December 2, 2009Publication date: April 19, 2012Inventor: Hugo Daniel Lujan
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Publication number: 20120083027Abstract: The invention is related to a method for purification of recombinant proteins using highly basic proteins from thermophilic bacteria as purification tags for use in a cation-exchange chromatography purification step. The basic proteins may be ribosomal proteins. The recombinant proteins are expressed in eukaryotic or prokaryotic host cells. The purification tag will typically have a pl above about 9 and comprise from about 15 to about 250 amino acid residues.Type: ApplicationFiled: December 1, 2011Publication date: April 5, 2012Applicant: Novo Nordisk A/SInventors: Allan Christian Shaw, Susanne Bang, Jing Su
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Patent number: 8148105Abstract: Methods are provided for the production of gram to kilogram quantities of pro-EP-B2 (proenzyme form of EP-B2) in a lyophilized form. The methods include scalable fermentation, refolding and purification processes, which processes may be combined with lyophilization to yield a stable product.Type: GrantFiled: March 14, 2008Date of Patent: April 3, 2012Assignees: The Board of Trustees of the Leland Stanford Junior University, Alvine Pharmaceuticals, Inc.Inventors: Harmit Vora, James McIntire, Pawan Kumar, Chaitan Khosla
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Publication number: 20120077285Abstract: The present invention relates to a method for purifying biomolecules or for analyzing whether an aqueous phase contains biomolecules by means of magnetic separation. The invention further relates to uses, to devices, and to kits that relate to the method according to the invention.Type: ApplicationFiled: March 25, 2010Publication date: March 29, 2012Inventor: Janina Cramer
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Patent number: RE43497Abstract: The present invention relates to methods for screening molecules and methods to detect protein-protein interactions and means used therein. More specifically, the present invention relates to methods for screening candidate drugs for treating or detecting MIF (macrophage migration inhibitor factor) related diseases. In certain aspects, the present invention involves detecting MIF/Jab1 (c-Jun activation domain binding protein) interactions as a basis for modulating cellular regulatory pathways and for identifying candidate drugs for MIF-related diseases. The invention also provides methods for the identification of molecules which dissociate or prevent interaction or binding between MIF and Jab1.Type: GrantFiled: November 2, 2000Date of Patent: June 26, 2012Assignee: Fraunhofer-Gesellschaft zur Forderung der Angewandten Forschung E.V.Inventors: Herwig Brunner, Jürgen Bernhagen, Robert Kleemann, Ralf Mischke, Afroditi Kapurniotu
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Patent number: RE43655Abstract: An improved process for the purification of antibodies from human plasma or other sources is disclosed. The process involves suspension of the antibodies at pH 3.8 to 4.5 followed by addition of caprylic acid and a pH shift to pH 5.0 to 5.2. A precipitate of contaminating proteins, lipids and caprylate forms and is removed, while the majority of the antibodies remain in solution. Sodium caprylate is again added to a final concentration of not less than about 15 mM. This solution is incubated for 1 hour at 25° C. to affect viral inactivation. A precipitate (mainly caprylate) is removed and the clear solution is diluted with purified water to reduce ionic strength. Anion exchange chromatography using two different resins is utilized to obtain an exceptionally pure IgG with subclass distribution similar to the starting distribution. The method maximizes yield and produces a gamma globulin with greater than 99% purity. The resin columns used to obtain a high yield of IgG retain IgM and IgA.Type: GrantFiled: October 17, 2007Date of Patent: September 11, 2012Assignee: Bayer HealthCare LLCInventors: Wytold R. Lebing, Douglas C. Lee, Klaus-Peter Radtke, Scott A. Cook, Hanns-Ingolf Paul, Patricia Alred