Physical Treatment Patents (Class 530/427)
  • Patent number: 7829681
    Abstract: Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides.
    Type: Grant
    Filed: April 7, 2008
    Date of Patent: November 9, 2010
    Assignee: Barofold Inc.
    Inventors: Matthew B. Seefeldt, Lyndal K. Hesterberg, Theodore W. Randolph, John F. Carpenter
  • Patent number: 7767795
    Abstract: The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
    Type: Grant
    Filed: January 27, 2006
    Date of Patent: August 3, 2010
    Assignee: BaroFold Inc.
    Inventors: Theodore W. Randolph, John F. Carpenter, Richard St. John
  • Publication number: 20100151525
    Abstract: Process for extracting hydrophobin from a solution wherein carrageenan is added to the solution and the pH of the solution is brought below 3.5, and the ionic strength of the solution is below 0.5.
    Type: Application
    Filed: December 11, 2009
    Publication date: June 17, 2010
    Applicant: CONOPCO, INC., D/B/A UNILEVER
    Inventor: Nicholas David Hedges
  • Publication number: 20100145021
    Abstract: A process for enhancing the recovery yield of proteins, especially plasma proteins, from sources containing the proteins, wherein the sources containing the proteins are frozen at temperatures of ??70° C., and the proteins from a frozen source after thawing are further processed in a per se known manner.
    Type: Application
    Filed: January 25, 2008
    Publication date: June 10, 2010
    Inventor: Wolfgang Marguerre
  • Publication number: 20100137567
    Abstract: The invention relates, for example, to methods for preparing a plant or animal sample for processing, i.e. for example for the isolation of nucleic acids or proteins from the sample, as well as to a pulverizer.
    Type: Application
    Filed: April 2, 2008
    Publication date: June 3, 2010
    Applicant: QIAGEN GMBH
    Inventor: Dennis Mertens
  • Patent number: 7728116
    Abstract: In a method of isolating osteogenic protein from bone, in which an osteogenic protein-containing fraction is extracted from bone and enriched by a sequence of enrichment steps selected from ultrafiltration and chromatography, the invention provides the improvement of removing higher molecular weight components from the osteogenic protein-containing fraction prior to the enrichment steps. The higher molecular weight components have a molecular weight of about 100-300 kDa and are selected from collagen, collagen fragments, collagen aggregates and mixtures thereof.
    Type: Grant
    Filed: June 18, 2003
    Date of Patent: June 1, 2010
    Assignee: Altis Biologics (Proprietary) Limited
    Inventor: Nicolaas Duneas
  • Publication number: 20100121032
    Abstract: Disclosed is a method for refolding a protein or peptide that does not contain essential disulfides and that contains at least one free cysteine residue. Also disclosed are polymer IFN-? conjugates that have been created by the chemical coupling of polymers such as polyethylene glycol moieties to IFN-?, particularly via a free cysteine in the protein. Also disclosed are analogs of bioactive peptides that may be used to create longer acting versions of the peptides, including analogs of glucagon, glucagon-like peptide-1 (GLP-1), GLP-2, Gastric inhibitory peptide (GIP), PYY, exendin, ghrelin, gastrin, amylin, and oxyntomodulin.
    Type: Application
    Filed: December 14, 2007
    Publication date: May 13, 2010
    Applicant: BOLDER BIOTECHNOLOGY, INC.
    Inventors: George N. Cox, Mary S. Rosendahl
  • Publication number: 20100098924
    Abstract: The present invention relates to a method for manufacturing a collagen membrane, which comprises the following steps: preparing a collagen slurry; degassing the collagen slurry, and then forming collagen gel at a predetermined collagen concentration, ionic strength, pH value, and temperature; removing water in the collagen gel by an absorbent device to form a collagen mat; and flattening and drying the collagen mat under vacuum by a gel dryer. The present invention provides the collagen membranes manufactured by the above-mentioned method. Such membranes are of good biocompatibility, high tensile strength, hydrophilicity, flexibility and easy handling features. Hence, these membranes can function as medical material suitable for repair of tissue and wound healing.
    Type: Application
    Filed: February 11, 2009
    Publication date: April 22, 2010
    Applicant: SunMax Biotechnology Co., Ltd.
    Inventors: Tzu-Yin Hsiao, Yu-Te Lin, Chien-Hsing Lin, Hsiang-Yin Lu
  • Patent number: 7691806
    Abstract: Biomolecular conjugates are provided which comprise the conjugation product of a repeat sequence protein polymer and at least one active agent. Additional aspects provide methods for their manufacture and various industrial and consumer applications.
    Type: Grant
    Filed: February 10, 2006
    Date of Patent: April 6, 2010
    Assignee: Genencor International, Inc.
    Inventors: Katherine D. Collier, William A. Cuevas, Manoj Kumar
  • Publication number: 20100075399
    Abstract: The present invention discloses improved methods of disaggregating protein aggregates, and refolding denatured proteins, using high pressure. In particular, the present invention provides for the use of agitation, high temperature, “stepped” depressurization, dialysis and dilution under pressure to increase the speed and extent of aggregate dissolution and protein refolding.
    Type: Application
    Filed: April 17, 2009
    Publication date: March 25, 2010
    Inventors: Theodore W. RANDOLPH, John F. Carpenter, Richard J. St. John, Jonathan N. Webb
  • Patent number: 7662922
    Abstract: A new canola protein isolate is provided along with a new canola protein. The new canola protein isolate is obtained from the supernatant from the production of a canola protein micellar mass and contains a predominance of 2S protein, The canola protein isolate derived from PMM contains a predominance of a 7S protein. Compositions of the canola protein isolate are provided.
    Type: Grant
    Filed: April 15, 2003
    Date of Patent: February 16, 2010
    Assignee: Burcon Nutrascience (MB) Corp.
    Inventors: James Logie, Radka Milanova
  • Publication number: 20090306354
    Abstract: A method for processing unseparated egg shells is provided. The method includes placing the unseparated egg shells in a fluid tank containing a fluid mixture, applying cavitation to the fluid mixture to thereby assist in separating the egg shell membranes from the egg shells, and recovering the egg shell membranes. Preferably, the fluid mixture is recirculated to thereby provide for continuous processing of unseparated egg shells. The method may further include drying the egg shell membranes to produce dried egg shell membranes which may then be vacuum packaged for storage and/or transport. The dried egg shell membranes may then be subjected to an extraction process for extracting at least one type of polypeptide from the egg shell membranes. Collagen, hyaluronic acid, or amino acids of interest may be extracted from the egg shell membrane and purified for numerous uses.
    Type: Application
    Filed: July 30, 2009
    Publication date: December 10, 2009
    Applicant: BIOVA, L.L.C.
    Inventor: VLADIMIR VLAD
  • Publication number: 20090291062
    Abstract: The invention provides an aqueous formulation comprising water and a protein, and methods of making the same. The aqueous formulation of the invention may be a high protein formulation and/or may have low levels of conductivity resulting from the low levels of ionic excipients. Also included in the invention are formulations comprising water and proteins having low osmolality.
    Type: Application
    Filed: November 28, 2008
    Publication date: November 26, 2009
    Inventors: Wolfgang FRAUNHOFER, Annika Bartl, Hans-juergen Krause, Markus Tschoepe, Katharina Kaleta
  • Publication number: 20090281289
    Abstract: Apparatus and method for the extraction of material from glands of arthropods. The apparatus includes a container in which at least part of the glands are placed and a buffer solution at least partially immersing the glands. Gland material is collected in the material collection area of the container, e.g., by release of the material from the glands into the buffer solution and sedimentation of the material at the bottom of the container. The method involves removing from a body of the arthropod the gland containing at least partially the material, making an opening in an epithelium of the gland, and placing the gland in the container at least partially immersed in a buffer solution, so that the material exits the glands and sediments in the material collection area of the container.
    Type: Application
    Filed: June 18, 2009
    Publication date: November 12, 2009
    Applicant: SPINTEC ENGINEERING GMBH
    Inventors: Michael Rheinnecker, Stefan Kohlhaas, Rolf Zimmat
  • Publication number: 20090270599
    Abstract: An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide.
    Type: Application
    Filed: June 30, 2009
    Publication date: October 29, 2009
    Inventor: Lee Angros
  • Publication number: 20090233348
    Abstract: A method for the disruption of biological cells by means of a homogenizer device which a) comprises an orifice plate having at least one inlet nozzle and an orifice plate having at least one exit nozzle, wherein, in the intermediate space between the orifice plates, a static mixer is situated and, if appropriate, mechanical energy is additionally introduced or b) comprises an orifice plate having at least one inlet nozzle and a baffle plate, wherein, in the intermediate space between the orifice plate and the baffle plate, if appropriate a static mixer is situated and/or mechanical energy is introduced.
    Type: Application
    Filed: August 6, 2007
    Publication date: September 17, 2009
    Applicant: BASF SE
    Inventors: Thomas Danner, Tillman Faust, Ulrike Richter, Thomas Subkowski, Marvin Karos
  • Patent number: 7589184
    Abstract: The invention provides compositions and methods for storage of biomolecules. The biomolecules are stored via absorption to a substrate. Absorbed biomolecules can be eluted or recovered from the substrate at a future time, and optionally be subjected to a subsequent analysis or application. Biomolecules absorbed to a substrate for storage may also optionally be preserved, i.e., the absorbed biomolecule is resistant to or resists degradation.
    Type: Grant
    Filed: May 24, 2005
    Date of Patent: September 15, 2009
    Assignee: GenVault Corporation
    Inventors: Michael Hogan, James C. Davis
  • Publication number: 20090215998
    Abstract: Disclosed are devices for holding samples, particularly liquid samples, during high-pressure treatment. The devices enable a variety of functions, such as high-throughput screening of samples in multi-compartment device embodiments, and adjustment of solution conditions during high-pressure treatment. The devices are designed to maintain integrity during the high-pressure conditions, and are optionally substantially impermeable to oxygen.
    Type: Application
    Filed: November 21, 2006
    Publication date: August 27, 2009
    Inventors: Jeffrey D. Antman, Ryan L. Crisman, Lyndal K. Hesterberg, Robert Nelson, Theodore W. Randolph, Matthew B. Seefeldt
  • Patent number: 7538198
    Abstract: The present invention discloses improved methods of disaggregating protein aggregates, and refolding denatured proteins, using high pressure. In particular, the present invention provides for the use of agitation, high temperature, “stepped” depressurization, dialysis and dilution under pressure to increase the speed and extent of aggregate dissolution and protein refolding.
    Type: Grant
    Filed: April 29, 2003
    Date of Patent: May 26, 2009
    Assignee: Barofold, Inc.
    Inventors: Theodore W. Randolph, John F. Carpenter, Richard J. St. John, Jonathan N. Webb
  • Patent number: 7521417
    Abstract: A process is disclosed for the preparation of an essentially tetramer-free, substantially stroma-free, polymerized, pyridoxylated hemoglobin. Also disclosed is an essentially tetramer-free, substantially stroma-free, polymerized, pyridoxylated hemoglobin product capable of being infused into human patients in an amount of up to about 5 liters.
    Type: Grant
    Filed: January 10, 2008
    Date of Patent: April 21, 2009
    Assignee: Northfield Laboratories, Inc.
    Inventors: Richard E. DeWoskin, Marc D. Doubleday
  • Publication number: 20090081751
    Abstract: A composition is disclosed with the composition comprising a mixture of natively glycosylated mammalian biological molecules produced by electromagnetically stimulating living mammalian cells.
    Type: Application
    Filed: April 23, 2008
    Publication date: March 26, 2009
    Inventors: Thomas J. Goodwin, Donnie Rudd
  • Patent number: 7498412
    Abstract: The subject invention provides a process for preparing a porous collagen matrix from connective tissue, said process comprising: a porous structure forming step to treat said connective tissue with poring agent in situ; and a washing step to remove the impurity from said porous connective tissue thereby obtaining a porous collagen matrix.
    Type: Grant
    Filed: March 8, 2006
    Date of Patent: March 3, 2009
    Inventors: Lynn L. H. Huang, Gin-Mol Liu
  • Publication number: 20090036656
    Abstract: A method of preparing a biomaterial comprises steps of acellularizing the fish scale, decalcifying the fish scale, and cleaning the fish scale and then extruding the fish scales.
    Type: Application
    Filed: April 9, 2008
    Publication date: February 5, 2009
    Applicant: BODY ORGAN BIOMEDICAL CORP.
    Inventors: Horng-Ji LAI, Chien-Cheng LIN, Shang-Ming LIN
  • Publication number: 20080305549
    Abstract: The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.
    Type: Application
    Filed: June 8, 2007
    Publication date: December 11, 2008
    Inventors: Michael P. Caulfield, Richard E. Reitz, Shuguang Li, Gloria Kwangja Lee, Ronald Krauss, Patricia J. Blanche, W. Henry Benner, Earl Cornell
  • Publication number: 20080300386
    Abstract: Methods of extracting a component of interest from a plurality of components are described.
    Type: Application
    Filed: June 2, 2008
    Publication date: December 4, 2008
    Applicant: PRESSURE BIOSCIENCES INC.
    Inventors: ALEXANDER LAZAREV, VERA GROSS
  • Publication number: 20080293926
    Abstract: The present invention relates to a method of separating a target from a liquid, which includes providing a polymer in an aqueous liquid, which polymer comprises at least one hydrophobic portion; contacting the polymer-containing liquid with the liquid comprising the target; applying a stimulus to the resulting mixture; and maintaining it until a reversible phase separation is obtained. One phase is polymer-rich and contains target(s) and another phase is polymer-poor. By either maintaining the stimulus, or applying a different stimulus and maintaining it, the polymer-rich phase is transformed into a substantially solid phase.
    Type: Application
    Filed: December 21, 2006
    Publication date: November 27, 2008
    Applicant: GE HEALTHCARE BIO-SCIENCES AB
    Inventors: Elisabeth Hallgren, Ronnie Palmgren, Linda Svensson
  • Publication number: 20080292635
    Abstract: The present invention relates to a protein found in natural rubber that can induce an allergic reaction in persons who have been sensitised to it. The invention provides for the process of isolating and purifying the protein and describes the characteristics of the protein, including its molecular weight, isoelectric point, amino acid sequence and allergenicity. The invention also describes the isolation and cloning a the DNA that encodes the protein. The production of the recombinant version of the protein using a protein expression vector is described.
    Type: Application
    Filed: December 11, 2007
    Publication date: November 27, 2008
    Applicant: MALAYSIAN RUBBER BOARD
    Inventors: Siti Arija Mad Arif, Nyu Ping Chew, Hoong Yeet Yeang
  • Patent number: 7423132
    Abstract: A method for the separation and assay of phosphorylated peptides or proteins from complex mixtures of proteins and peptides is described. The method uses a diazo moiety linked by an organic group to a substrate. Kits for performing the assay are also described. The method and kits are particularly useful for detecting changes in phosphorylates produced in living cells.
    Type: Grant
    Filed: June 7, 2005
    Date of Patent: September 9, 2008
    Assignee: Board of Trustees of Michigan State University
    Inventors: Jetze J. Tepe, Thomas J. Pinnavaia
  • Patent number: 7411044
    Abstract: A substantially tetramer free hemoglobin solution and a method for producing a substantially tetramer free hemoglobin solution. The method includes polymerizing a solution of hemoglobin, treating the polymerized hemoglobin solution to partially degrade the polymer to tetramer and removing tetramer from the hemoglobin solution.
    Type: Grant
    Filed: August 24, 2006
    Date of Patent: August 12, 2008
    Assignee: Northfield Laboratories, Inc.
    Inventors: Anthony Avella, Richard DeWoskin, Marc Doubleday
  • Patent number: 7385037
    Abstract: Method for the preparation of gliadin- and glutenin-rich fractions from gluten in an aqueous medium and in the presence of an acid, wherein the gluten are dispersed continuously or not in water up to a dry substance varying between 5 and 30%, by which the pH of the dispersion is monitored between 4.4 and 4.8 and the gluten-water mixture is submitted to shearing actions, through which the dispersion, continuously or not, can be fractionated in gliadin- and glutenin-rich fractions, by which a single gliadin-rich fraction with a gliadin/glutenin ratio of at least 2.5 is obtained, and a single glutenin-rich fraction with a gliadin/glutenin ratio of less than 0.8 is obtained.
    Type: Grant
    Filed: July 30, 2002
    Date of Patent: June 10, 2008
    Assignee: Tate & Lyle Europe
    Inventor: Stefaan Roels
  • Publication number: 20080132688
    Abstract: The present invention relates, in general, to methods for removing viral contaminants from therapeutic protein solutions to improve safety of therapeutic proteins administered to patients. Particularly contemplated is the removal of small non-enveloped viruses, such as parvovirus, from therapeutic protein solutions.
    Type: Application
    Filed: September 21, 2007
    Publication date: June 5, 2008
    Applicant: AMGEN INC.
    Inventor: Joe Zhou
  • Patent number: 7368542
    Abstract: The binding specificity of at least one plasma protein suspended or dissolved in a liquid medium is altered by exposing the protein to an oxidizing agent or an electric current sufficient to alter its binding specificity. A masked protein such as an autoantibody can be recovered from blood or blood products or extracts by oxidizing the protein to change its binding specificity.
    Type: Grant
    Filed: June 9, 2004
    Date of Patent: May 6, 2008
    Assignee: Redox-Reactive Reagents LLC
    Inventor: John A. McIntyre
  • Patent number: 7358342
    Abstract: A preparation derived from shark cartilage for the treatment of diseases related to excessive PHF or Excessive intracellular calcium.
    Type: Grant
    Filed: April 16, 2004
    Date of Patent: April 15, 2008
    Assignee: fX Life Sciences International GmbH
    Inventors: Peter K. T. Pang, Jacqueline J. Shan, Kam Chiu
  • Patent number: 7244828
    Abstract: A method of protein precipitation, concentration and removal of non-protein agents from the protein solution wherein the protein solution is treated with a protein-precipitation agent containing an acidic agent, a salt and a precipitate forming agent. After precipitation, the protein precipitate is washed with a water miscible organic solvent agent to remove non-protein agents present in the protein precipitate.
    Type: Grant
    Filed: February 3, 2005
    Date of Patent: July 17, 2007
    Inventor: Aftab Alam
  • Patent number: 7135553
    Abstract: A substantially tetramer free hemoglobin solution and a method for producing a substantially tetramer free hemoglobin solution. The method includes polymerizing a solution of hemoglobin, treating the polymerized hemoglobin solution to partially degrade the polymer to tetramer and removing tetramer from the hemoglobin solution.
    Type: Grant
    Filed: January 29, 2004
    Date of Patent: November 14, 2006
    Assignee: Northfield Laboratories, Inc.
    Inventors: Anthony Avella, Richard DeWoskin, Marc Doubleday
  • Patent number: 7122642
    Abstract: A method to prepare new or unexpected polymorphs of materials which have not been observed, or to obtain a known polymorph under different conditions than those in which it is usually made, by using a laser to cause nucleation and crystal growth to occur in a supersaturated solution in such a way as to obtain a crystal structure which would not normally appear without the use of the laser.
    Type: Grant
    Filed: January 24, 2002
    Date of Patent: October 17, 2006
    Assignee: Polytechnic University
    Inventors: Allan S. Myerson, Bruce A. Garetz
  • Patent number: 7119166
    Abstract: A universal folding method that has been demonstrated to be effective in refolding a variety of very different proteins expressed in bacteria as inclusion bodies has been developed. Representative proteins that can be dissolved and refolded in biologically active form, with the native structure, are shown in Table I. The method has two key steps to unfold and then refold the proteins expressed in the inclusion bodies. The first step is to raise the pH of the protein solution in the presence of denaturing agents to pH greater than 9, preferably 10. The protein solution may be maintained at the elevated pH for a period of up to about 24 hours, or the pH immediately decreased slowly, in increments of about 0.2 pH units/24 hours, until the solution reaches a pH of about 8.0, or both steps used. In the preferred embodiment, purified inclusion bodies are dissolved in 8 M urea, 0.1 M Tris, 1 mM glycine, 1 mM EDTA, 10 mM beta-mercaptoethanol, 10 mM dithiothreitol (DTT), 1 mM reduced glutathione (GSH), 0.
    Type: Grant
    Filed: April 17, 2003
    Date of Patent: October 10, 2006
    Assignee: Oklahoma Medical Research Foundation
    Inventor: Xinli Lin
  • Patent number: 7115719
    Abstract: Reagents, methods and kits for denaturation of protein are provided.
    Type: Grant
    Filed: December 15, 2004
    Date of Patent: October 3, 2006
    Assignee: Gentra Systems, Inc.
    Inventor: Kim E. Paulsen
  • Patent number: 7109300
    Abstract: A method is disclosed for extracting collagen from calcified tissue without a prior decalcification step. The method may be used with calcified tissues such as fish skin with scales, alligator skeletons, and crustacean exoskeletons. It does not require the use of EDTA. When fish skin is used that contains substantial quantities of scales, it is not necessary first to remove scales from the skin before conducting the extraction of collagen.
    Type: Grant
    Filed: April 18, 2005
    Date of Patent: September 19, 2006
    Assignee: Board of Supervisors of Louisiana State Univeristy And Agricultural and Mechanical College
    Inventors: Jack N. Losso, Masahiro Ogawa, Ralph J. Portier, Mark A. Schexnayder
  • Patent number: 7098315
    Abstract: A method of preparing a collagen sponge comprises mixing air into a collagen gel, so as to obtain a collagen foam which is dried. From the dried product thereby obtained, collagen sponge is obtained by isolating parts of sponge with a chamber diameter of more than 0.75 mm and less than 4 mm, or parts with an average chamber diagonal dimension of 3 mm. The collagen sponge may be used as a material for sealing wounds, possibly with a coating comprising a fibrin glue, such as a combination of fibrinogen, thrombin and aprotinin. A device for extracting a part of a collagen foam and for degenerating another part of the collagen foam to a collagen gel is disclosed. An elongated collagen sponge having a through-going hole or bore and a flexible wall may be used for re-establishing walls in a mammalian gastrointestinal funnel or trachea system.
    Type: Grant
    Filed: January 25, 2002
    Date of Patent: August 29, 2006
    Assignee: Nycomed Pharma AS
    Inventor: Alfred Schaufler
  • Patent number: 7064192
    Abstract: The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
    Type: Grant
    Filed: November 12, 2002
    Date of Patent: June 20, 2006
    Assignee: The Regents of the University of Colorado
    Inventors: Theodore W. Randolph, John F. Carpenter, Richard St. John
  • Patent number: 7034128
    Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.
    Type: Grant
    Filed: October 29, 2004
    Date of Patent: April 25, 2006
    Assignee: Large Scale Biology Corporation
    Inventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
  • Patent number: 7022822
    Abstract: Purification process of humanPurification process of human urinary gonadotropins of high biological activity and chemical purity absolutely free of foreign contaminating materials derived from the use of biological reagents or chromatography dyes, from crude of gonadotropins. The high biological activity and chemically pure composition of human gonadotropins obtained by this process, are used for the treatment of infertility and are selected from the group of follitropin or menotropins, having a bioactivity greater than 2500 IU/mg protein as tested by biological assay in rats, for both FSH and LH hormones for menotropins and greater than 5000 IU/mg protein for follitropin having an FSH:LH ratio about 75:1. Pharmaceutical preparations of said gonadotropins free of these contaminating materials are also comprised within the present invention.
    Type: Grant
    Filed: April 14, 2000
    Date of Patent: April 4, 2006
    Assignee: Instituto Massone S.A.
    Inventors: Claudio Fernando Wolfenson Band, Liliana Ester Balanian, Jose-Felipe Groisman, Erundina Marta Fasanella
  • Patent number: 6995246
    Abstract: The present invention provides soluble protein solutions, free of suspended particles in high yield. More particularly, the current invention provides a method for removing suspended particles from soluble protein solutions by filtering the soluble protein solution through highly purified diatomaceous earth.
    Type: Grant
    Filed: February 22, 2001
    Date of Patent: February 7, 2006
    Assignee: Akzo Nobel N.V.
    Inventors: Min Wan, Susan M Rabideau
  • Patent number: 6967239
    Abstract: Method for preparing a factor VIII solution that is essentially free of viruses and essentially devoid of vWF (von Willebrand factor) and factor VIII-vWF complexes by (a) obtaining a starting factor VIII solution devoid of factor VIII-vWF complexes; and (b) filtering the solution through a hydrophilic virus filter.
    Type: Grant
    Filed: December 14, 1998
    Date of Patent: November 22, 2005
    Assignee: Laboratoire Francias du Franctionnement et des Biotechnologies
    Inventors: Abdessatar Chtourou, Michel Nogre, Pierre Porte
  • Patent number: 6924151
    Abstract: There are disclosed a stable factor VIII/vWF-complex, particularly comprising high-molecular vWF multimers, being free from low-molecular vWF molecules and from proteolytic vWF degradation products, as well as a method of producing this complex.
    Type: Grant
    Filed: May 7, 2001
    Date of Patent: August 2, 2005
    Assignee: Baxter Aktiengesellschaft
    Inventors: Bernhard Fischer, Artur Mitterer, Friedrich Dorner, Johann Eibl
  • Patent number: 6894029
    Abstract: Compositions of substantially homogeneous type II-like collagen from invertebrates belonging to the class Scyphozoa, phylum Coelenterata, i.e., jellyfish, particularly Stomolophus meleagris, and methods for its extraction are provided. Methods for the treatment of arthritis, in particular rheumatoid arthritis, by administering an effective amount of the collagen-containing compositions of the invention so as to induce immune tolerance, are also provided.
    Type: Grant
    Filed: November 13, 2001
    Date of Patent: May 17, 2005
    Assignee: Auburn University
    Inventor: Yun-Hwa Peggy Hsieh
  • Patent number: 6875617
    Abstract: A method of protein precipitation, concentration and removal of non-protein agents from the protein solution wherein the protein solution is treated with a protein-precipitation agent containing an acidic agent, a salt and a precipitate forming agent. After precipitation, the protein precipitate is washed with a water miscible organic solvent agent to remove non-protein agents present in the protein precipitate.
    Type: Grant
    Filed: April 27, 2001
    Date of Patent: April 5, 2005
    Assignee: Geno Technology, Inc.
    Inventor: Aftab Alam
  • Patent number: 6844424
    Abstract: This invention provides a method for separating and extracting biological products of avian origin. The method allows for the production of avian cartilages and of active ingredients which can be extracted from the cartilages thus obtained.
    Type: Grant
    Filed: August 27, 1999
    Date of Patent: January 18, 2005
    Assignee: Diana Ingredients
    Inventors: Laurent Mollard, Agnes Montillet, Cecile Horriere, Jack Legrand, Tan Hung Nguyen
  • Patent number: RE39497
    Abstract: A material or mixture of materials which is not itself storage stable is rendered storage stable by incorporation into a water-soluble or swellable glassy or rubbery composition which can then be stored at ambient temperature. Recovery is by adding aqueous solution to the composition.
    Type: Grant
    Filed: August 28, 2001
    Date of Patent: February 27, 2007
    Assignee: Nektar Therapeutics
    Inventors: Felix Franks, Ross H. M. Hatley