Physical Treatment Patents (Class 530/427)
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Patent number: 6844321Abstract: Crystals of glucagon-like peptide-1 (GLP-1) and GLP-1 analogues, and processes for preparation of crystals of GLP-1 and GLP-1 analogues.Type: GrantFiled: January 25, 2001Date of Patent: January 18, 2005Assignee: Novo Nordisk A/SInventor: Anne Charlotte Arentsen
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Patent number: 6841659Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: August 1, 2003Date of Patent: January 11, 2005Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Patent number: 6780979Abstract: The invention concerns a method for purifying PrPres from a biological sample to be used for qualitative and/or quantitative determination of the PrPres in said sample. The method essentially consists in: (1) incubating, during 30 seconds to 2 hours, at a temperature less than 80° C.Type: GrantFiled: October 13, 2000Date of Patent: August 24, 2004Assignee: Commissariat a l'Energie AtomiqueInventor: Jean-Philippe Deslys
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Patent number: 6759521Abstract: A method to select and prepare polymorphs of materials by switching the polarization state of light employing non-photochemical laser-induced nucleation.Type: GrantFiled: September 28, 2001Date of Patent: July 6, 2004Inventors: Allan S. Myerson, Bruce A. Garetz
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Patent number: 6756482Abstract: The present invention provides a process for purifying human activin by cation exchange chromatography and chaotropic ion concentration gradient elution.Type: GrantFiled: November 27, 2000Date of Patent: June 29, 2004Assignee: Ajinomoto Co., Inc.Inventors: Kunio Ono, Shigekatsu Tsuchiya, Daisuke Ejima, Yuzuru Eto
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Patent number: 6747132Abstract: The present invention employs a filtration step during the hemoglobin purification process that substantially decreases viral contamination of a hemoglobin solution. The filtration means can be used to separate hemoglobin and several endogenous antioxidant enzymes from red blood cell stroma and potential adventitious agents. The purified hemoglobin/antioxidant composition is then subjected to a chemical modification process. The resulting modified hemoglobin/antioxidant composition is then fractionated to remove unmodified hemoglobin species and residual reactants, formulated in electrolytes and rendered sterile. The resulting modified hemoglobin product is substantially free of viral contamination and contains at least one endogenous antioxidant enzyme that retains antioxidant activity.Type: GrantFiled: August 16, 2001Date of Patent: June 8, 2004Assignee: Apex Biosciences, Inc.Inventors: Christopher Thomas Privalle, Cyrus John Stacey, Todd Lewis Talarico
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Patent number: 6740740Abstract: The present invention features a method for isolating and purifying proteins and peptides of interest from a plant host, which is applicable on a larger scale. Moreover, the present invention provides a more efficient method for isolating proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target protein/peptide from other components of the green juice by one or more cycles of centrifugation and/or resuspension, and finally purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.Type: GrantFiled: September 24, 2001Date of Patent: May 25, 2004Assignee: Large Scale Biology CorporationInventors: Stephen J. Garger, R. Barry Holtz, Michael J. McCulloch, Thomas H. Turpen
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Patent number: 6734210Abstract: Stable salts of azelaic acid with polycations such as chitosan are described. The salts according to the invention are water-soluble, therapeutically more efficacious and are valuable for use as active constituents in pharmaceutical as well as cosmeceutical compositions.Type: GrantFiled: February 23, 2001Date of Patent: May 11, 2004Inventor: Rolland F. Hebert
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Patent number: 6670454Abstract: The present invention relates to crosslinking of porous materials made of biodegradable polymers. The method comprises: (a) placing a porous biodegradable polymer in a chamber; (b) introducing a supercritical fluid containing a crosslinking agent into the chamber to effect crosslinking of the porous biodegradable polymer; and optionally (c) introducing a pure supercritical into the chamber to wash the crosslinked polymer until the crosslinking agent is substantially removed from the polymer.Type: GrantFiled: November 1, 2001Date of Patent: December 30, 2003Assignee: Industrial Technology Research InstituteInventors: Huey-Min Lai, Kuang-Rong Lee, Chin-Chin Tsai, Hsi-Hsin Shih, Yuan-Chia Chang
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Patent number: 6660495Abstract: The present invention relates to a process for the controlled enzymatic cleavage of purified and depigmented active allergenic proteins from indoor and outdoor source materials, which process produces fragments of allergens that retain the natural T-lymphocyte stimulating epitopes, but are depleted of IgE-binding B-cell epitopes and complement-activating agents. The invention also relates to the new pharmaceutical products. These allergen fragments do not exhibit the disadvantages of conventional allergenic extracts for immunotherapy and can be safely used to induce a state of specific T-cell anergy and immunological tolerance in allergic human beings.Type: GrantFiled: December 4, 2001Date of Patent: December 9, 2003Assignee: C.B.F. Leti, S.A.Inventors: Lubertus Berrens, Maria Leticia Gonzales Romano, Maria Teresa Gallego Camara
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Patent number: 6617435Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: April 8, 2002Date of Patent: September 9, 2003Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Patent number: 6610831Abstract: Methods and apparatus for recovering zein from substrates are disclosed. The method includes extracting a zein-containing substrate such as whole corn with ethanol to yield a crude zein alcoholic dispersion and treating this dispersion with an adsorbent to remove at least one of starch, color or oil to yield a purified zein which is subsequently recovered or used in industrial applications. A preferred adsorbent is activated charcoal.Type: GrantFiled: December 21, 1999Date of Patent: August 26, 2003Assignee: Lurgi PSI, Ltd.Inventors: Jerel McInnis, Qingnong Tang
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Patent number: 6602985Abstract: Zein is recovered from gluten meal prepared by wet milling procedures by washing the gluten with clean water to remove water-soluble components; separating the water-soluble components and recovering the water-insoluble components; extracting the water insoluble components with hydrous ethanol solvent to extract zein; recovering the crude zein extract; treating the crude zein extract with an adsorbent that adsorbs at least one of color, odor, oil and fatty acid; and to yield a purified zein extract.Type: GrantFiled: February 10, 2000Date of Patent: August 5, 2003Assignee: Lurgi PSI, Inc.Inventors: Jerel McInnis, Qingnong Tang
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Patent number: 6590081Abstract: Pure, stable crystalline forms of parathyroid hormone, particularly teriparatide, are described as well as methods of preparation and purification.Type: GrantFiled: May 31, 2000Date of Patent: July 8, 2003Assignee: Eli Lilly and CompanyInventor: Faming Zhang
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Patent number: 6579723Abstract: A method for purifying factor VIII/vWF complex or free vWF by immunoaffinity chromatography in a form suitable for use as a medicament. Factor VIII/vWF complex or free vWF is recovered from an immunoaffinity adsorbent by using an eluting agent containing a zwitterionic species. The presence of the zwitterionic species allows for the use of mild conditions throughout the preparation, facilitating retention of molecular integrity, activity, and incorporation of the recovered proteins into pharmaceutical preparations without the need for additional stabilizers or preservatives.Type: GrantFiled: October 21, 1999Date of Patent: June 17, 2003Assignee: Baxter AktiengesellschaftInventors: Artur Mitterer, Christian Fiedler, Bernhard Fischer, Friedrich Dorner, Johann Eibl
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Patent number: 6569999Abstract: A method for activating a denatured protein, includes the steps of: adding a detergent to the denatured protein to allow a protein-detergent complex to be formed; and adding high-molecular weight amylose to the protein-detergent complex so that the high-molecular weight amylose removes the detergent.Type: GrantFiled: March 2, 2001Date of Patent: May 27, 2003Assignee: National Food Research Institute, Ministry of Agriculture, Forestry and FisheriesInventors: Sachiko Machida, Kiyoshi Hayashi, Takeshi Takaha, Yoshinobu Terada, Kazutoshi Fujii
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Patent number: 6566490Abstract: Disclosed is a method for producing seeding microcrystals for the production of human insulin, the microcrystals being free of non-human pancreatic insulin, the method comprising providing an unseeded suspension of human insulin, the suspension being free of non-human pancreatic insulin, and homogenizing the insulin suspension under pressure to result in human insulin microcrystals suitable for use as seeding microcrystals for the production of zinc insulin products. The method of homogenization under pressure may also be used for the production of seeding mnicrocrystals for other peptides and proteins, in particular pharmaceutical peptides or proteins such as insulin, GLP-1, glucagon and growth hormones.Type: GrantFiled: December 20, 2000Date of Patent: May 20, 2003Assignee: Novo Nordisk A/SInventors: Flemming Manique, Christian Ilsoe
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Patent number: 6562952Abstract: A process for forming small micron-sized (1-10 &mgr;m) protein particles is provided wherein a protein, a solvent system for the protein and an antisolvent for the protein solvent system are contacted under conditions to at least partially dissolve the protein solvent system in the antisolvent, thereby causing precipitation of the protein. The solvent system is made up of at least in part of a halogenated organic alcohol, most preferably 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP). Preferably, a solution of the protein in the solvent system is sprayed through a nozzle into a precipitation zone containing the antisolvent (preferably CO2) under near- or supercritical conditions.Type: GrantFiled: October 31, 2000Date of Patent: May 13, 2003Assignee: The University of KansasInventors: Roger A. Rajewski, Bala Subramaniam, William K. Snavely, Fenghui Niu
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Publication number: 20030027997Abstract: Crystal growth can be initiated and controlled by dynamically controlled vapor diffusion or temperature change. In one aspect, the present invention uses a precisely controlled vapor diffusion approach to monitor and control protein crystal growth. The system utilizes a humidity sensor and various interfaces under computer control to effect virtually any evaporation rate from a number of different growth solutions simultaneously by means of an evaporative gas flow. A static laser light scattering sensor can be used to detect aggregation events and trigger a change in the evaporation rate for a growth solution. A control/follower configuration can be used to actively monitor one chamber and accurately control replicate chambers relative to the control chamber. In a second aspect, the invention exploits the varying solubility of proteins versus temperature to control the growth of protein crystals.Type: ApplicationFiled: October 9, 2002Publication date: February 6, 2003Inventors: Terry L. Bray, Larry J. Kim, Michael Harrington, Lawrence J. Delucas, Wilbur William Wilson
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Patent number: 6506725Abstract: A blood substitute and plasma expander comprising a cross-linked, substantially endotoxin-free hemoglobin solution and process for preparing same. The process comprises fractionating whole blood, separating out a stromal-free, sterile hemoglobulin solution, chromatographically separating endotoxins from said hemoglobin solution and crosslinking the resulting endotoxin-free hemoglobin solution.Type: GrantFiled: May 10, 1999Date of Patent: January 14, 2003Assignee: BioPure CorporationInventors: Carl W. Rausch, Mario Feola
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Patent number: 6504085Abstract: A method for extraction of heterologous protein from plant seed comprises extracting the germ portion of the seed and extracting and purifying the protein from the germ. Enhanced expression in the germ is provided, and allows for improved efficiency in production, and cost savings. Directing expression to the germ portion further increases expression levels of the protein. The ubiquitin promoter preferentially directs expression to the germ portion of plant seed.Type: GrantFiled: September 7, 1999Date of Patent: January 7, 2003Assignee: ProdiGene, Inc.Inventor: John A. Howard
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Patent number: 6498141Abstract: A process is disclosed for the preparation of an essentially tetramer-free, substantially stroma-free, polymerized, pyridoxylated hemoglobin. Also disclosed is an essentially tetramer-free, substantially stroma-free, polymerized, pyridoxylated hemoglobin product capable of being infused into human patients in an amount of up to about 5 liters.Type: GrantFiled: May 10, 1999Date of Patent: December 24, 2002Assignee: Northfield Laboratories, Inc.Inventors: Richard E. DeWoskin, Marc D. Doubleday
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Patent number: 6489450Abstract: The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.Type: GrantFiled: July 9, 1999Date of Patent: December 3, 2002Assignee: University Technology CorporationInventors: Theodore W. Randolph, John F. Carpenter, Richard St. John
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Patent number: 6479636Abstract: A method of extracting and purifying recombinant protein(s) from transgenic sugarcane is disclosed. Fractioning of sugarcane juice that has been extracted from the cane stalks is obtained by using a multiple stage filtering process that uses multiple stages of decreasing porosity (preferably screening) followed by preferably membrane type filters, ion exchange, membrane adsorber, and chromatographic processes.Type: GrantFiled: April 10, 2001Date of Patent: November 12, 2002Assignees: Honiron Corporation (a Louisiana Corporation), The Texas A&M University (an Agency of the State of Texas)Inventors: T. Erik Mirkov, Jean P. Monclin, Adam Barrilleaux, James E. Irvine, Francis Moonan
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Patent number: 6454950Abstract: A method for the separation of a predetermined compound comprising i) partitioning the compound in a two-phase (system A) in which at least one of the phases is rich (phase 1) and the other is poor (phase 2) in a thermoseparating polymer (I), ii) collecting one phase (phase 1 or phase 2) containing said compound, and iii if desired, further working up said compound from the phase collected in step ii, characterized a) in that polymer (I) is a micell-forming thermoseparating polymer, and b) in that phase 2 possibly contains at least one agent that is cloud point-decreasing for thermoseparating polymer (I), such as a polymer (II) that is incompatible with polymer (I) or a salt.Type: GrantFiled: October 3, 2001Date of Patent: September 24, 2002Assignee: Amersham Pharmacia Biotech ABInventors: Folke Tjerneld, Josefine Persson, Hans Olof Johansson
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Patent number: 6441147Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: November 28, 2000Date of Patent: August 27, 2002Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Patent number: 6437101Abstract: Methods are provided in this invention for the isolation of human growth hormone, growth hormone antagonist, or a homologue of either, from a biological source. The methods of the invention use multi-phase extraction.Type: GrantFiled: May 7, 1999Date of Patent: August 20, 2002Assignee: Akzo Nobel N.V.Inventors: Kirk James Hayenga, Pascal P. Valex
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Patent number: 6426406Abstract: A method to prepare new or unexpected polymorphs of materials which have not been observed, or to obtain a known polymorph under different conditions than those in which it is usually made, by using a laser to cause nucleation and crystal growth to occur in a supersaturated solution in such a way as to obtain a crystal structure which would not normally appear without the use of the laser.Type: GrantFiled: October 23, 2000Date of Patent: July 30, 2002Inventors: Allan S. Myerson, Bruce A. Garetz
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Patent number: 6387877Abstract: The invention relates to a method of removing endotoxin from preparations of alpha-1-acid glycoprotein (orosomucoid) by contact with a finely divided non-toxic resin such as fumed silica. The invention also relates to a purification process for alpha-1-acid glycoprotein which includes this deprogenation step, and to the depyrogenated product and its clinical uses.Type: GrantFiled: January 25, 1999Date of Patent: May 14, 2002Assignee: National Blood AuthorityInventors: John Edward More, Jacqueline Rott, David Roger Lewin
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Publication number: 20020025343Abstract: A process is disclosed for the preparation of an essentially tetramerfree, substantially stromafree, polymerized, pyridoxylated hemoglobin. Also disclosed is an essentially tetramerfree, substantially stromafree, polymerized, pyridoxylated hemoglobin product capable of being infused into human patients in an amount of up to about 5 liters.Type: ApplicationFiled: May 10, 1999Publication date: February 28, 2002Inventors: RICHARD E. DE WOSKIN, MARC D. DOUBLEDAY
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Patent number: 6350590Abstract: The present invention relates to a process for the controlled enzymatic cleavage of purified and depigmented active allergenic proteins from indoor and outdoor source materials, which process produces fragments of allergens that retain the the natural T-lymphocyte stimulating epitopes, but are depleted of IgE-binding B-cell epitopes and complement-activating agents. The invention also relates to the new pharmaceutical products. These allergen fragments do not exhibit the disadvantages of conventional allergenic extracts for immunotherapy and can be safely used to induce a state of specific T-cell anergy and immunological tolerance in allergic human beings.Type: GrantFiled: June 23, 2000Date of Patent: February 26, 2002Assignee: C.B.F. Leti, S.A.Inventors: Lubertus Berrens, Maria Leticia Gonzales Romano, Maria Teresa Gallego Camara
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Patent number: 6342367Abstract: Disclosed is an economical method for the preparation of chondroitin sulfates A and C useful as an effective ingredient of medicaments from fish scales as a waste material discharged from fishery in large quantities. Fish scales are enzymatically decomposed in an aqueous medium in the presence of a protease to isolate the chondroitin sulfate compounds and by-product polypeptides followed by removal of the by-product polypeptides from the aqueous solution by a cation-exchange treatment and then the aqueous solution of the chondroitin sulfate compounds is subjected to fractional precipitation by the addition of ethyl alcohol as the precipitant.Type: GrantFiled: February 25, 2000Date of Patent: January 29, 2002Assignee: Japan as represented by Secretary of Agency of Industrial Science and TechnologyInventors: Toshihisa Sumi, Hideki Ohba, Toru Ikegami, Masao Shibata, Tsuyoshi Sakaki, Imre Sallay, Sung Soo Park
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Patent number: 6337389Abstract: The present invention relates to a process for the production of marine invertebrate type V telopeptide containing collagen preparations from marine invertebrates, compositions containing preparations, and methods of using these preparations. The collagen preparation includes telopeptide containing and optionally invertebrate atelopeptide containing, type V fibrillar collagen. The present collagen preparations may be employed in a variety of products including for example, cosmetic, pharmacological, dental, and cell culture products.Type: GrantFiled: October 28, 1997Date of Patent: January 8, 2002Assignee: BioScience Consultants, L.L.C.Inventor: Lloyd Wolfinbarger, Jr.
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Publication number: 20010055617Abstract: The present invention concerns processes for the production of dry, partially amorphous products containing biologically active and in particular therapeutically active material which are macroscopically homogeneous substance mixtures, the substance mixtures being selected from at least one substance of each of the groupsType: ApplicationFiled: April 27, 1998Publication date: December 27, 2001Inventors: MARKUS MATTERN, GERHARD WINTER
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Patent number: 6326029Abstract: A resorbable extracellular matrix for reconstruction of cartilage tissue, the matrix being substantially free from non-native collagen, and including a purified collagen II material formed form natural cartilage and having fibers of native collagen II which are physiologically acceptable for implant into a mammalian body.Type: GrantFiled: November 10, 1997Date of Patent: December 4, 2001Assignee: Ed Geistlich Soehne AG fuer Chemische IndustrieInventors: Peter Geistlich, Myron Spector, Zdenek Eckmayer
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Patent number: 6323326Abstract: A method of modifying protein solubility employs polyionic polymers. These facilitate the solubilization, formulation, purification and refolding of proteins especially incorrectly folded proteins and aggregated proteins. Compositions are described that are suitable for formulating TFPI. The compositions allow preparation of pharmaceutically acceptable compositions of TFPI at concentrations above 0.2 mg/mL and above 10 mg/mL.Type: GrantFiled: June 11, 1999Date of Patent: November 27, 2001Assignees: Chiron Corporation, G. D. Searle & Co.Inventors: Glenn J. Dorin, Bo H. Arve, Gregory L. Pattison, Robert F Halenbeck, Kirk Johnson, Bao-Lu Chen, Rajsharan K. Rana, Maninder S. Hoba, Hassan Madani, Michael Tsang, Mark E. Gustafson, Gary S. Bild, Gary V. Johnson
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Patent number: 6319896Abstract: A method of modifying protein solubility employs polyionic polymers. These facilitate the solubilization, formulation, purification and refolding of proteins especially incorrectly folded proteins and aggregated proteins. Compositions are described that are suitable for formulating TFPI. The compositions allow preparation of pharmaceutically acceptable compositions of TFPI at concentrations above 0.2 mg/mL and above 10 mg/mL.Type: GrantFiled: November 18, 1999Date of Patent: November 20, 2001Assignees: Chiron Corporation, G.D. Searle & Co.Inventors: Glenn J. Dorin, Bo H. Arve, Gregory L. Pattison, Robert F. Halenbeck, Kirk Johnson, Bao-Lu Chen, Rajsharan K. Rana, Maninder S. Hora, Hassan Madani, Michael Tsang, Mark E. Gustafson, Gary S. Bild, Gary V. Johnson
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Patent number: 6313268Abstract: This invention is directed to a novel &bgr;-secretase that produces the A&bgr; peptide found in Alzheimer's Disease. One &bgr;-secretase is a protein having a molecular weight of about 61, 81 or 88 kDa that cleaves an amyloid precursor protein (APP) substrate. Another is a protease complex having a molecular between about 180 and 200 kDa, which, in one embodiment, contains the 61, 81, and 88 kDa proteins and, in another embodiment, contains proteins having a molecular weight of about 66, 60, 33 and 29 kDa. Another &bgr;-secretase has a molecular weight between about 50 and 90 kDA. The invention is also directed to methods of selecting agents that inhibit A&bgr; peptide production and treating Alzheimer's disease in patients.Type: GrantFiled: April 20, 1999Date of Patent: November 6, 2001Inventor: Vivian Y. H. Hook
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Publication number: 20010034066Abstract: A method of protein precipitation, concentration and removal of non-protein agents from the protein solution wherein the protein solution is treated with a protein-precipitation agent containing an acidic agent, a salt and a precipitate forming agent. After precipitation, the protein precipitate is washed with a water miscible organic solvent agent to remove non-protein agents present in the protein precipitate.Type: ApplicationFiled: April 27, 2001Publication date: October 25, 2001Inventor: Aftab Alam
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Patent number: 6284875Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.Type: GrantFiled: February 9, 2000Date of Patent: September 4, 2001Assignee: Large Scale Biology CorporationInventors: Thomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Patent number: 6281337Abstract: The present invention provides methods for isolating adjunct isoforms of proteins and converting them to the desired protein. In preferred embodiments, the present invention contemplates the use of acid solutions or zinc solutions to cleave a chemical group from a desired protein. In further preferred embodiments, the present invention contemplates the oxidation of reduced sulfhydryl groups with cleavage of chemical groups to form a functional desired protein.Type: GrantFiled: November 12, 1999Date of Patent: August 28, 2001Assignee: Schering CorporationInventors: Susan Cannon-Carlson, Andres Frei, Seoju Lee, Roland Mengisen, Marcio Voloch, David C. Wylie
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Patent number: 6239262Abstract: A method for purification of proteins by displacement chromatography in hydrophobic interaction and reversed phase chromatographic systems uses low molecular weight (less than about 10,000) surface-active compounds as displacers. Examples of effective displacers are benzethonium chloride, benzyltributylammonium chloride, and tetrahexylammonium chloride.Type: GrantFiled: December 30, 1998Date of Patent: May 29, 2001Assignee: Rensselaer Polytechnic InstituteInventors: Steven M. Cramer, Abhinav A. Shukla, Khurram M. Sunasara
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Publication number: 20010001657Abstract: One can impart outstanding resistance against collapse under pressure to gas-filled microvesicle used as contrast agents in ultrasonic echography by using as fillers gases whose solubility in water, expressed in liter of gas by liter of water under standard conditions, divided by the square root of the molecular weight does not exceed 0.003.Type: ApplicationFiled: April 4, 1997Publication date: May 24, 2001Inventors: MICHEL SCHNEIDER, FENG YAN, PASCAL GRENIER, NADINE GARCEL, JEROME PUGINIER, MARIE-BERNADETTE BARRAU
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Patent number: 6210574Abstract: A system for separating nucleated cells from a blood sample includes a charge-flow separator (CFS), which separates blood into fractions according to the surface charge density characteristics coupled with an affinity-filtration separator which either outputs a separated blood fraction to the CFS or receives a separated blood fraction from the CFS. The system permits separation of nucleated fetal red blood cells, erythroid progenitor cells and other nucleated cells found in blood samples.Type: GrantFiled: May 24, 1999Date of Patent: April 3, 2001Assignee: BioSeparations, Inc.Inventors: David W. Sammons, Garland E. Twitty, Joseph G. Utermohlen, Rizwan Sharnez
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Patent number: 6197935Abstract: The use of collagen as a biomedical implant raises safety issues towards viruses and prions. The physicochemical changes and the in vitro and in vivo biocompatibility of collagen treated with heat, and by formic acid (FA), trifluoroacetic acid (TFA), tetrafluoroethanol (TFE) and hexafluoroiso-propanol (HFIP) were investigated. FA and TFA resulted in extensive depurination of nucleic acids while HFIP and TFE did so to a lesser degree. The molecules of FA, and most importantly of TFA, remained within collagen. Although these two acids induced modification in the secondary structure of collagen, resistance to collagenase was not affected and, in vitro, cell growth was not impaired. Severe dehydrothermal treatment, for example 110° C. for 1-3 days under high vacuum, also succeeded in removing completely nucleic acids. Since this treatment also leads to slight cross-linking, it could be advantageously used to eliminate prion and to stabilize gelatin products.Type: GrantFiled: September 29, 1998Date of Patent: March 6, 2001Assignee: DiagnoCure, Inc.Inventors: Charles Doillon, Régen Drouin, Gaétan LaRoche
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Patent number: 6162904Abstract: A process for producing an intravenously-administrable gamma globulin solution substantially free of contaminating viruses by heat treating for viral inactivation and fractionating an impure gamma globulin solution and then treating the purified gamma globulin with a solvent-detergent for further viral inactivation. In a continuous process disclosed herein, partially purified gamma globulin solids is not recovered as an intermediate product during the disclosed process. In the continuous process, the fractionation to obtain a purified gamma globulin solution is carried out without precipitation of the desired gamma globulin.Type: GrantFiled: October 21, 1999Date of Patent: December 19, 2000Assignee: Alpha Therapeutic CorporationInventors: Raja R. Mamidi, Andranik Bagdasarian, Gorgonio Canaveral, Kazuo Takechi
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Patent number: 6160097Abstract: A process is described for reactivating purified membrane proteins in which a mixture composed of membrane protein, a phospholipid and a detergent is frozen and subsequently thawed.Type: GrantFiled: January 12, 2000Date of Patent: December 12, 2000Assignee: Dade Behring Marburg GmbHInventor: Norbert F. Zander
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Patent number: 6136959Abstract: A process for isolating edible protein from animal muscle by solubilizing the protein in an alkaline aqueous solution is disclosed.Type: GrantFiled: June 22, 1998Date of Patent: October 24, 2000Assignee: University of MassachusettsInventors: Herbert O. Hultin, Stephen D. Kelleher
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Patent number: RE37872Abstract: A material or mixture of materials which is not itself storage stable is rendered storage stable by incorporation into a water-soluble or swellable glassy or rubbery composition which can then be stored at ambient temperature. Recovery is by adding aqueous solution to the composition.Type: GrantFiled: March 17, 1999Date of Patent: October 8, 2002Assignee: Inhale Therapeutics Systems, Inc.Inventors: Felix Franks, Ross H. M. Hatley
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Patent number: RE38385Abstract: A material or mixture of materials which is not itself storage stable is rendered storage stable by incorporation into a water-soluble or swellable glassy or rubbery composition which can then be stored at ambient temperature. Recovery is by adding aqueous solution to the composition.Type: GrantFiled: August 28, 2001Date of Patent: January 13, 2004Assignee: Nektar TherapeuticsInventors: Felix Franks, Ross H. M. Hatley