Encodes An Enzyme Patents (Class 536/23.2)
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Patent number: 9387192Abstract: The present invention relates to a combined preparation comprising at least one rocaglamide derivative and at least one apoptosis inducing agent, preferably from the group of substances comprising agents inducing the extrinsic apoptotic pathway, antiproliferative agents and agents which induce apoptosis in T-cells by activation induced cell death (AICD) for the treatment of cancer.Type: GrantFiled: November 20, 2009Date of Patent: July 12, 2016Assignee: DKFZ DEUTCHERS KREBSFORSCHUNGSZENTRUMInventors: Min Li-Weber, Peter H. Krammer
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Patent number: 9309501Abstract: Isolated DNA polymerase and the mutant DNA polymerases thereof are provided. The DNA polymerases have good thermostability.Type: GrantFiled: December 23, 2013Date of Patent: April 12, 2016Assignee: Industrial Technology Research InstituteInventors: Tseng-Huang Liu, Pei-Shin Jiang, Chih-Lung Lin, Su-Jan Lee, Chao-Hung Kao
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Patent number: 9297028Abstract: Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway.Type: GrantFiled: September 27, 2006Date of Patent: March 29, 2016Assignee: Butamax Advanced Biofuels LLCInventors: Gail K. Donaldson, Lixuan Lisa Huang, Lori Ann Maggio-Hall, Vasantha Nagarajan, Charles E. Nakamura, Wonchul Suh
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Patent number: 9273100Abstract: The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptides and cyclic peptide production in mushrooms. In particular, the present invention relates to using genes and proteins from Galerina species encoding peptides specifically relating to amatoxins in addition to proteins involved with processing cyclic peptide toxins. In a preferred embodiment, the present invention also relates to methods for making small peptides and small cyclic peptides including peptides similar to amanitin. Further, the present inventions relate to providing kits for making small peptides.Type: GrantFiled: January 31, 2012Date of Patent: March 1, 2016Assignee: Board of Trustees of Michigan State UniversityInventors: Heather E. Hallen-Adams, John S. Scott-Craig, Jonathan D. Walton, Hong Luo
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Patent number: 9273104Abstract: The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium.Type: GrantFiled: November 15, 2013Date of Patent: March 1, 2016Assignees: NOVOZYMES, INC., NOVOZYMES A/SInventors: Mads Bjornvad, Debbie Yaver
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Patent number: 9260729Abstract: The invention provides a non-naturally occurring microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.Type: GrantFiled: June 24, 2011Date of Patent: February 16, 2016Assignee: Genomatica, Inc.Inventors: Jun Sun, Priti Pharkya, Anthony P. Burgard
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Patent number: 9212361Abstract: The present invention relates to an inhibitor of Neutrophil Gelatinase-Associated Lipocalin (NGAL) activity or expression for use in a method for treating or preventing hypertension in a subject in need thereof.Type: GrantFiled: April 18, 2013Date of Patent: December 15, 2015Assignees: Institut National de la Sante et de la Recherche Medicale (INSERM), Universite Paris Diderot (Paris VII), Universite Paris DescartesInventors: Frederic Jaisser, Nicolette Farman, Antoine Tarjus
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Patent number: 9212371Abstract: The present invention pertains to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.Type: GrantFiled: May 11, 2010Date of Patent: December 15, 2015Assignee: BASF Plant Science Company GmbHInventors: Toralf Senger, Sten Stymne, Jenny Lindberg Yilmaz
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Patent number: 9187736Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.Type: GrantFiled: February 15, 2013Date of Patent: November 17, 2015Assignee: E I DU PONT DE NEMOURS AND COMPANYInventors: Knut Meyer, Howard Glenn Damude, Kevin G. Ripp, Kevin L. Stecca
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Patent number: 9181555Abstract: An isolated polypeptide comprising a hydrogen generating enzyme attached to a heterologous ferredoxin is disclosed, as well as polynucleotides encoding same, nucleic acid constructs capable of expressing same and cells expressing same. A method for generating hydrogen using the isolated polypeptide is also disclosed.Type: GrantFiled: July 23, 2008Date of Patent: November 10, 2015Assignee: Ramot at Tel-Aviv University Ltd.Inventors: Iftach Yacoby, Ehud Gazit, Nathan Nelson, Itai Benhar
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Patent number: 9180149Abstract: The present invention concerns methods and compositions for the treatment of cancer and cancer cells using intravascular administration of a vaccinia virus. In some embodiments, methods and compositions involve a replicative vaccinia virus that encodes GM-CSF.Type: GrantFiled: September 7, 2006Date of Patent: November 10, 2015Assignee: SILLAJEN BIOTHERAPEUTICS, INC.Inventor: David Kirn
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Patent number: 9175274Abstract: Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (?)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (?)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.Type: GrantFiled: August 27, 2014Date of Patent: November 3, 2015Assignee: University of Kentucky Research FoundationInventors: Chang-Guo Zhan, Fang Zheng, Wenchao Yang
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Patent number: 9175299Abstract: The present invention is directed to nucleic acids encoding polypeptides that confer upon a plant tolerance to an imidazolinone and/or other acetohydroxyacid synthase (AHAS) inhibiting herbicide when expressed in the plant. The present invention also provides plants having increased tolerance to an imidazolinone and/or other AHAS-inhibiting herbicide. More particularly, the present invention includes plants containing at least one IMI nucleic acid. The present invention also includes seeds produced by these plants and methods of controlling weeds in the vicinity of these wheat plants.Type: GrantFiled: December 1, 2005Date of Patent: November 3, 2015Assignee: BASF AGROCHEMICAL PRODUCTS, B.V.Inventors: John Moffatt, Rob Bruns, Iwona Birk, Bijay Singh
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Patent number: 9120871Abstract: Expression of FGF21 in a S. cerevisiae pmt2 knock out strain reduces the O-glycosylation significantly and does not decrease the expression level. Expression in mkc7 knock out strain can decrease degradation of FGF21 and analogues thereof. Point mutations at position R17 and R36 can decrease degradation of FGF21 and analogues thereof when they are expressed in yeast.Type: GrantFiled: January 20, 2011Date of Patent: September 1, 2015Assignee: Novo Nordisk A/SInventors: Rita Slaaby, Inger Lautrup-Larsen
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Patent number: 9074224Abstract: Disclosed herein are methods and compositions for gene targeting utilizing fusion molecules comprising a recombinase domain and a sequence-specific DNA-binding domain.Type: GrantFiled: August 26, 2010Date of Patent: July 7, 2015Assignee: Recombinetics, Inc.Inventors: Jeffrey J. Essner, Hsin-Kai Liao, Scott C. Fahrenkrug
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Publication number: 20150150157Abstract: Prephenate dehydrogenases and arogenate dehydrogenase polynucleotide and polypeptide sequences are provided herein. These polypeptides are all insensitive to effector based feedback inhibition. Polypeptides with these activities and lacking feedback inhibition by the product were not previously identified and characterized from plants. The polypeptides may be used to generate constructs and transgenic cells or plants. Methods of increasing production of products of the tyrosine or HPP pathway by increasing expression of the polynucleotides provided herein in plants or cells overexpressing the polypeptides are provided. In addition overexpression of the polypeptides in plant cells increases resistance to herbicides.Type: ApplicationFiled: November 19, 2014Publication date: May 28, 2015Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Hiroshi A. Maeda, Craig Albert Schenck
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Publication number: 20150150156Abstract: The present disclosure relates to recombinant methyltransferases that epigenetically silence gene expression and to methods of using such proteins for reducing the expression of genes in plants.Type: ApplicationFiled: January 11, 2013Publication date: May 28, 2015Inventors: Steve E. Jacobsen, Elena Caro
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Publication number: 20150147800Abstract: The present invention relates to a method for the enzymatic decarboxylation of malonic acid (propanedioic acid) derivatives catalyzed by enzymes structurally and/or functionally related to arylmalonate decarboxylase (AMDase) as isolated from microorganisms of the genus Bordetella. The present invention also relates to novel enzymes with a decarboxylase activity, useful for performing the claimed method, mutants thereof, corresponding coding sequences and expression systems, methods of preparing said novel enzymes, and screening methods for obtaining further suitable enzymes also having said decarboxylase activity.Type: ApplicationFiled: December 29, 2014Publication date: May 28, 2015Inventors: Bernhard Hauer, Nina Schneider, Krzysztof Okrasa, Jason Micklefield, David Leys, Colin Levy
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Publication number: 20150147365Abstract: The present invention relates to an oral composition comprising a volatile sulphur compound breath-odour controlling amount of methyl mercaptan oxidase. The oral composition may be used in a method for controlling breath-odour in a host, which method comprises applying the composition to the oral cavity of that host. The invention also provides a polypeptide having methyl mercaptan oxidase activity, wherein said polypeptide is: (a) a polypeptide comprising an amino acid sequence as set out in SEQ ID NO: 2; (b) a polypeptide comprising an amino acid sequence having at least 50% sequence identity with the amino acid sequence of SEQ ID NO: 2; (c) a polypeptide encoded by a polynucleotide comprising the polynucleotide sequence as set out in SEQ ID NO: 1 or SEQ ID NO: 3; or (d) a fragment of a polypeptide as defined in (a), (b) or (c).Type: ApplicationFiled: May 13, 2013Publication date: May 28, 2015Inventors: Luppo Edens, Evert Tjeerd Van Rij, Marco Jan Lambertus De Groot
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Patent number: 9040238Abstract: The invention refers to polynucleotides selected from the group consisting of a) polynucleotides encoding for the polypeptide RBM20 comprising a P638L mutation for a human polypeptide RBM20, or a P641L mutation for a rat polypeptide RBM20, b) polynucleotides with a reverse complementary sequence of the polynucleotide of a) above, and c) polynucleotides with an identity at least 50% to a polynucleotide of a) or b) above.Type: GrantFiled: June 22, 2010Date of Patent: May 26, 2015Assignee: MAX-DELBRÜCK-CENTRUM FÜR MOLEKULARE MEDIZIN BERLIN-BUCHInventors: Michael Gotthardt, Norbert Hübner, Marion Lewis Greaser, Wei Guo
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Patent number: 9040278Abstract: An ?-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.Type: GrantFiled: June 4, 2009Date of Patent: May 26, 2015Assignee: DANISCO US INC.Inventors: Luis G. Cascao-Pereira, Regina Chin, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Michael J. Pepsin, Scott D. Power, Sandra W. Ramer, Carol A. Requadt, Andrew Shaw, Amr R. Toppozada, Louise Wallace
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Patent number: 9040279Abstract: The present disclosure relates to a Bacillus subtilis alpha-amylase (AmyE) or its variants thereof. AmyE or its variants thereof may be used to more efficiently produce fermentable sugars from starch. Also disclosed are a composition comprising a glucoamylase and AmyE or variant thereof and a method of processing starch utilizing the described enzyme composition.Type: GrantFiled: June 4, 2009Date of Patent: May 26, 2015Assignee: DANISCO US INC.Inventors: Suzanne Breneman, Sung Ho Lee, Vivek Sharma, Jayarama K. Shetty
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Patent number: 9040262Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: May 4, 2011Date of Patent: May 26, 2015Assignee: Codexis, Inc.Inventors: Michael Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga Modukuru, Emily Mundorff, Derek Smith, Shiwei Song, Wan Lin Yeo
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Patent number: 9040269Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.Type: GrantFiled: April 17, 2008Date of Patent: May 26, 2015Assignee: Evola SAInventors: Michael Katz, Hans Peter Smits, Jochen Förster, Jens Bredal Nielsen
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Publication number: 20150139975Abstract: The present invention relates to recombinant factor H and variants and conjugates thereof and methods of their production, as well as uses and methods of treatment involving the materials.Type: ApplicationFiled: November 14, 2014Publication date: May 21, 2015Inventors: Christoph SCHMIDT, Paul N. BARLOW, Anna RICHARDS
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Publication number: 20150140588Abstract: The present invention relates to variants with improved activity in an amide-bond reaction. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: July 3, 2012Publication date: May 21, 2015Applicant: Novozymes A/SInventors: Werner Besenmatter, Allan Svendsen
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Publication number: 20150140572Abstract: The present invention relates to a composition having the activity of degrading the cell wall of a Mycobacterium species comprising: (a) a first fusion protein including (i) a domain with a first enzymatic activity, the enzymatic activity being at least one or more of the following: N-acetyl-b-D-muramidase (lysozyme, lytic transglycosylase), N-acetyl-b-D-glucosaminidase, N-acetylmuramoyl-L-alanine amidase, L-alanoyl-D-glutamate (LD) endopeptidase, c-D-glutamyl-meso-diaminopimelic acid (DL) peptidase, D-Ala-m-DAP (DD) endopeptidase, or m-DAP-m-DAP (LD) endopeptidase, (ii) at least one peptide stretch fused to the N- or C-terminus of the domain with the first enzymatic activity; and (iii) a protein transduction domain (PTD) being at the N- or C-terminus of the first fusion protein; and (b) a second fusion protein including (i) a domain with a second enzymatic activity, the enzymatic activity being at least one or more of the following: lipolytic activity, cutinase, mycolarabinogalactanesterase, or alpha/beta hyType: ApplicationFiled: July 1, 2013Publication date: May 21, 2015Inventors: Stefan Miller, Robert Andreas Fischer
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Publication number: 20150141633Abstract: The disclosure relates to a method for enhancing the biosynthesis and/or secretion of sapogenins in the culture medium of plant and microbial cell cultures. Further, the disclosure also relates to the identification of novel genes involved in the biosynthesis of sapogenin intermediates, as well as to novel sapogenin compounds.Type: ApplicationFiled: May 13, 2013Publication date: May 21, 2015Inventors: Alain Goossens, Tessa Moses, Jacob Pollier, Lorena Almagro Romero
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Publication number: 20150140641Abstract: The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.Type: ApplicationFiled: November 14, 2014Publication date: May 21, 2015Inventors: XIYUN ZHANG, SACHIN PATIL, JIE YANG, ISH KUMAR DHAWAN
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Publication number: 20150140587Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.Type: ApplicationFiled: July 14, 2014Publication date: May 21, 2015Inventors: Aldis Darzins, Lance Encell, Tonny Johnson, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
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Publication number: 20150140132Abstract: The object of the present invention is to provide Humulus lupulus-derived monoterpene glycosyltransferase and a method for producing a monoterpene glycoside by means of this enzyme. The present invention provides Humulus lupulus-derived monoterpene glycosyltransferase and a method for producing a monoterpene glycoside by means of this enzyme. The present invention provides a transformant transformed with a gene for Humulus lupulus-derived monoterpene glycosyltransferase and a method for producing such a transformant.Type: ApplicationFiled: February 5, 2013Publication date: May 21, 2015Inventors: Eiichiro Ono, Nobuo Tsuruoka
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Publication number: 20150140636Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.Type: ApplicationFiled: May 22, 2014Publication date: May 21, 2015Applicant: Promega CorporationInventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
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Publication number: 20150141287Abstract: Genetic markers associated with intellectual disability as well as compositions, methods and kits for screening for genetic markers of intellectual disability, diagnosing intellectual disability and identifying individuals with a predisposition for offspring suffering from intellectual disability are provided.Type: ApplicationFiled: January 22, 2015Publication date: May 21, 2015Inventor: John B. Vincent
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Publication number: 20150140169Abstract: The invention relates to a polypeptide having chymosin activity which: a. is capable of hydrolysing bovine alpha s1-casein at position F23F24 so as to form ?s1-I CN (f24-199) more rapidly than camel chymosin; and b. has a C/P ratio higher than the C/P ratio of bovine chymosin.Type: ApplicationFiled: May 3, 2013Publication date: May 21, 2015Inventors: Petrus Jacobus Theodorus Dekker, René Marcel De Jong, Michael Dennis Tabeling, Cornelis Marinus Muijlwijk
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Publication number: 20150140574Abstract: A modified polynucleotide has a different base sequence in at least one codon from a wild-type base sequence encoding a horseradish peroxidase polypeptide. The usage frequency of the modified codon of the polynucleotide corresponds to the codon usage frequencies of three filamentous fungal species in Humicola, Aspergillus, and Trichoderma. The polynucleotide is capable of expressing the polypeptide to be encoded in a filamentous fungus.Type: ApplicationFiled: May 30, 2013Publication date: May 21, 2015Applicant: MEIJI SEIKA PHARMA CO., LTD.Inventors: Fumikazu Yokoyama, Kaoru Okakura, Atsushi Inoue, Koichiro Murashima, Toshiaki Nagasato, Koji Yanai, Akitaka Nakane
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Patent number: 9034629Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described.Type: GrantFiled: January 27, 2014Date of Patent: May 19, 2015Assignee: Joule Unlimited Technologies, Inc.Inventors: Frank Anthony Skraly, Ning Li
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Patent number: 9034616Abstract: A method of producing butanal by optimizing the growth of Streptomyces using cellulose as food source, overexpressing a key ‘gate’ enzyme in butyric acid/butyraldehyde production, and knocking out the isobutyryl-CoA synthase gene to shunt the pathway. Optionally, the produced butanal can be isolated and converted into butanol.Type: GrantFiled: April 6, 2010Date of Patent: May 19, 2015Assignee: Syracuse UniversityInventors: Robert Doyle, Joshua Lensbouer, Anthony Vortherms
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Patent number: 9034598Abstract: Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.Type: GrantFiled: May 16, 2011Date of Patent: May 19, 2015Assignees: aTyr Pharma, Inc., Pangu BioPharma LimitedInventors: Leslie Ann Greene, Kyle P. Chiang, Fei Hong, Alain Philippe Vasserot, Jeffry D. Watkins, Cheryl L. Quinn, Wing-Sze Lo, John D. Mendlein
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Patent number: 9034608Abstract: The invention relates to a polypeptide having a mutation at one or more position corresponding to T219 of SEQ ID NO: 55, wherein the polypeptide has at least 50% sequence identity with SEQ ID NO: 55, and wherein the polypeptide has permease activity.Type: GrantFiled: October 11, 2011Date of Patent: May 19, 2015Assignee: DSM IP ASSETS, B.V.Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paul Klaassen, Rene Marcel De Jong
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Patent number: 9034611Abstract: The present invention is related to a microorganism for the production of methionine, wherein said microorganism is modified to enhance the transhydrogenase activity of PntAB. In a preferred aspect of the invention, the activity of the transhydrogenase UdhA is attenuated in said microorganisms. The invention also related to a method for producing methionine by fermentation.Type: GrantFiled: October 24, 2011Date of Patent: May 19, 2015Assignee: METALBOLIC EXPLORERInventors: Wanda Dischert, Perrine Vasseur, Cédric Boisart, Rainer Figge
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Publication number: 20150132820Abstract: An object is to efficiently produce thermostable catalase at low cost by expressing it as a recombinant protein in large quantity. A recombinant microorganism capable of efficiently expressing thermostable catalase can be provided by obtaining a DNA necessary for efficiently producing it as a recombinant protein, and the thermostable catalase can be efficiently produced at low cost by cultivating the obtained recombinant microorganism. Hydrogen peroxide can be efficiently decomposed at low cost, even at high temperature, by treating a solution containing hydrogen peroxide with the thermostable catalase of the present invention.Type: ApplicationFiled: January 27, 2015Publication date: May 14, 2015Applicant: MEIJI SEIKA PHARMA CO., LTD.Inventors: Kaoru OKAKURA, Fusuke MAZUKA, Takayoshi FUKUSHIMA, Koichiro MURASHIMA
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Publication number: 20150132806Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.Type: ApplicationFiled: January 27, 2015Publication date: May 14, 2015Inventors: Emily Mundorff, Erik Jan De Vries
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Publication number: 20150132368Abstract: The present invention relates to compositions and methods for delivering lysosomal proteins. The compositions and methods described herein permit the targeted delivery of exogenous lysosomal proteins to cell surface proteins that allow their internalization via non-clathrin pathways. The present invention further relates to the use 10 of the compositions and methods for enzyme replacement therapy of lysosomal storage diseases. Nucleic acids, recombinant cells and kits useful for making and using the compositions of the invention are also provided.Type: ApplicationFiled: October 16, 2014Publication date: May 14, 2015Inventors: Silvia Muro Galindo, Vladimir R. Muzykantov, Edward Howard Schuchman
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Publication number: 20150132807Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.Type: ApplicationFiled: November 12, 2014Publication date: May 14, 2015Inventors: Nicholas J. Agard, Oscar Alvizo, Melissa Ann Mayo, Stefanie Ng Minor, James Nicholas Riggins, Jeffrey C. Moore
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Publication number: 20150132824Abstract: The present invention relates to isolated polypeptides having xanthan degrading activity, catalytic domains and polynucleotides encoding the polypeptides and catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and catalytic domains.Type: ApplicationFiled: May 7, 2013Publication date: May 14, 2015Inventors: Dorotea Raventos Segura, Peter Fischer Halin, Anders Viksoe-Nielsen, Lars Anderson, Martin Simon Borchert, Leigh Murphy, Astrid Boisen, Lorena G. Palmén, Kenneth Jensen, Carsten Sjoeholm, Tine Hoff, Charlotte Blom
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Method for producing an L-amino acid belonging to the glutamate family, using a coryneform bacterium
Patent number: 9029104Abstract: The present invention provides a method for producing an L-amino acid belonging to the glutamate family, using a coryneform bacterium which has been modified so that expression of one or more gene(s) of the NCgl_2067-NCgl_2065 operon in said bacterium is/are attenuated.Type: GrantFiled: October 3, 2013Date of Patent: May 12, 2015Assignee: Ajinomoto Co., Inc.Inventors: Svetlana Alekseevna Samsonova, Viktor Vasilievich Samsonov, Yulia Georgievna Rostova, Mikhail Markovich Gusyatiner -
Patent number: 9029122Abstract: Methods for increasing C18 to C20 elongation conversion efficiency and/or ?4 desaturation conversion efficiency in long-chain polyunsaturated fatty acid [“LC-PUFA”]-producing recombinant oleaginous microbial host cells are provided herein, based on over-expression of acyl-CoA:lysophospholipid acyltransferases [“LPLATs”] (e.g., Ale1, LPAAT, LPCAT). Production host cells and oils produced by the methods of the invention are also claimed.Type: GrantFiled: August 2, 2013Date of Patent: May 12, 2015Assignee: E I du Pont de Nemours and CompanyInventors: Narendra S Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Patent number: 9029116Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: August 30, 2013Date of Patent: May 12, 2015Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
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Patent number: 9029142Abstract: Gene encoding human glucokinase mutant is provided. The gene has the nucleotide sequence chosen from the nucleotide sequence listed as SEQ ID NO:2 and the nucleotide sequence wherein the ORF region encodes the same amino acid sequence as the one encoded by ORF region (position 487 to 1884) of SEQ ID NO:2 and the rest of the region is same as the non-ORF region of SEQ ID NO:2. Human glucokinase mutant encoded by the gene, the recombinant vectors carrying the gene, the hosts comprising the vectors, pharmaceutical compositions thereof, uses thereof, and methods for treating and preventing diseases by using the same are provided. The human glucokinase mutant encoded by the gene has higher activity than that of the wild type human glucokinase, and thus provides a new way of controlling blood glucose and/or preventing and/or treating disturbance of carbohydrate metabolism, especially preventing and treating diabetes.Type: GrantFiled: July 7, 2011Date of Patent: May 12, 2015Inventor: Haidong Huang
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Patent number: 9029107Abstract: It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a hydrolase protein, which consists of the amino acid sequence shown in any one of SEQ ID NOS. 2 to 5 and which has activity of catalyzing, at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO. 1, a reaction of producing (1S,2S)-1-ethoxycarbonyl-2-vinylcyclopropanecarboxylic acid from diethyl 2-vinylcyclopropane-1,1-dicarboxylate.Type: GrantFiled: August 31, 2011Date of Patent: May 12, 2015Assignee: API CorporationInventors: Hiroshi Kawabata, Ryoma Miyake, Kuniko Asada, Ryouhei Katou