Fungal Protein Patents (Class 536/23.74)
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Publication number: 20130183713Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 30, 2011Publication date: July 18, 2013Applicants: NOVOYZMES A/S, NOVOZYMES, INC.Inventor: Marc Morant
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Publication number: 20130180013Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 30, 2011Publication date: July 11, 2013Applicants: NOVOYZMES A/S, NOVOZYMES, INC.Inventor: Nikolaj Spodsberg
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Publication number: 20130160156Abstract: The present invention concerns methods for controlling and/or preventing fungus infestation on a cell, organism, substrate or material via dsRNA mediated gene silencing. The methods of the invention are particularly used to alleviate pathogenic fungal infestation on plants, plant materials or seeds. Suitable fungal target genes and fragments thereof, expression cassettes, dsRNA molecules, host cells expressing the dsRNA, compositions and transgenic plants and plant cells are provided.Type: ApplicationFiled: June 8, 2011Publication date: June 20, 2013Applicant: Devgen Private LimitedInventors: Simone Dorfmueller, Phuay-Yee Goh, Pascale Feldmann
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Patent number: 8465958Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: August 30, 2012Date of Patent: June 18, 2013Assignee: Novozymes A/SInventors: Alfredo Lopez de Leon, Michael Rey
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Publication number: 20130152231Abstract: The present invention provides methods and compositions relating to altering NT activity, nitrogen utilization and/or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low or normal nitrogen fertility. The invention provides isolated nitrate transporter (NT) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NT enzyme show improved properties, for example, increased yield.Type: ApplicationFiled: February 19, 2013Publication date: June 13, 2013Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventor: Pioneer Hi-Bred International, Inc.
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Patent number: 8461320Abstract: The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. Knockout, mutants wherein a gene associated with metabolism is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.Type: GrantFiled: April 22, 2003Date of Patent: June 11, 2013Assignee: DuPont Nutrition Biosciences ApSInventors: Leendert Hendrik De Graaff, Henriëtta Catharina Van Den Broeck, Jacob Visser
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Publication number: 20130133107Abstract: Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.Type: ApplicationFiled: January 23, 2013Publication date: May 23, 2013Applicant: ATHENIX CORPORATIONInventor: Athenix Corporation
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Publication number: 20130108642Abstract: The invention provided Mucorales CotH polypeptides and encoding nucleic acid molecules. The Mucorales CotH polypeptides and encoding nucleic acids can be advantageously used to diagnose, treat or prevent fungal conditions, in particular mucormycosis.Type: ApplicationFiled: September 14, 2012Publication date: May 2, 2013Inventors: Ashraf S. Ibrahim, Mingfu Liu, Teklegiorgis Ghebremariam, Yue Fu, John E. Edwards, Scott Filler
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Publication number: 20130104263Abstract: The present invention relates to a method for identifying and/or characterising an endophyte strain, said method including providing a plurality of samples of endophytes, subjecting said endophytes to genetic analysis, subjecting said endophytes to metabolic analysis and selecting endophytes having a desired genetic and metabolic profile. The present invention also relates to novel endophytes having a desired toxin profile wherein the endophyte produces significantly less toxic alkaloids compared with a control endophyte such as standard toxic (ST) endophyte; and/or significantly more alkaloids conferring beneficial properties compared with a control endophyte such as ST endophyte. The present invention also relates to endophyte variants having a desired genetic and metabolic profile, wherein said endophyte variants possess genetic and/or metabolic characteristics that result in a beneficial phenotype in a plant harbouring or otherwise associated with the endophyte variant.Type: ApplicationFiled: July 6, 2012Publication date: April 25, 2013Applicant: AGRICULTURE VICTORIA SERVICES PTY LTDInventors: German Carlos Spangenberg, Kathryn Michaela Guthridge, John White Forster, Timothy Ivor Sawbridge, Emma Jane Isobel Ludlow, Jatinder Kaur, Simone Jane Rochfort, Maia Andrea Rabinovich, Piyumi Ekanayake
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Patent number: 8399736Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.Type: GrantFiled: December 16, 2011Date of Patent: March 19, 2013Assignee: E.I. du Pont de Nemours and CompanyInventors: Knut Meyer, Howard Glenn Damude, Kevin G. Ripp, Kevin L. Stecca
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Patent number: 8394383Abstract: The invention relates to seven allergens of Aspergillus versicolor which are suitable for specific diagnostics of this mold which predominantly pollutes the interior. The invention is also directed to an immunological method of detecting a sensitization to A. versicolor, especially an allergy, wherein one or more of the above-mentioned seven allergens found are employed as diagnostic markers. The invention further relates to a pharmaceutical composition which includes one or more of these seven allergens as active substance, and to the use of one or more of these seven allergens for the desensitization to A. versicolor.Type: GrantFiled: July 3, 2008Date of Patent: March 12, 2013Assignee: Helmholtz-Zentrum fur Umwelt-Forschung GmbHInventors: Andrea Müller, Dirk Benndorf, Katharina Bock, Martin Von Bergen, Olaf Herbarth
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Publication number: 20130059389Abstract: An object to be solved by the present invention is to provide, for example, a method for producing a Kluyveromyces marxianus transformant by a method for conveniently and efficiently connecting the ends of DNA fragments without using specific restriction enzymes or their recognition sequences, and a method for producing a useful substance using the transformant.Type: ApplicationFiled: February 1, 2011Publication date: March 7, 2013Applicant: YAMAGUCHI UNIVERSITYInventors: Rinji Akada, Hisashi Hoshida, Babiker Mohamed Ahmed Abdel-Banat, Jun Asakawa
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Publication number: 20130017554Abstract: A new SUMO-specific affinity tag is described herein, based on the amino acid sequence from 403-621 of the SUMO protease Ulp1, and containing a crucial substitution of serine for cysteine. This affinity tag is particularly useful for a range of applications, including detection and affinity purification of sumoylated proteins from cell extracts.Type: ApplicationFiled: June 22, 2012Publication date: January 17, 2013Applicant: COLLEGE OF WILLIAM AND MARYInventors: Oliver Kerscher, Megan Donaher, Zachary C. Elmore
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Publication number: 20130011876Abstract: The invention relates to a process for preparing filamentous fungal strains having a sexual cycle, wherein an acceptor filamentous fungal strain, having no or one type MAT locus, is subjected to recombination in which one or more mating type locus genes and optionally sex related genes, from other species than the acceptor filamentous fungal strain, is introduced into the acceptor filamentous fungal strain to produce a filamentous fungal strain having a sexual cycle.Type: ApplicationFiled: January 12, 2011Publication date: January 10, 2013Applicant: DSM IP ASSETTS, B.V.Inventors: Peter Jozef Ida Van De Vondervoort, Herman Jan Pel, Paul Stanley Dyer
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Patent number: 8350123Abstract: The present invention provides transgenic cover crop plants which after harvest degrade the hemicellulose and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise cellulase, hemicellulase and optionally ligninase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular, the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the hemicellulase and cellulase which then degrade the hemicellulose and cellulose of the transgenic plants to produce the fermentable sugars.Type: GrantFiled: October 19, 2011Date of Patent: January 8, 2013Assignee: Board of Trustees of Michigan State UniversityInventor: Masomeh B. Sticklen
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Publication number: 20120311741Abstract: The invention relates to a transporter protein involved in the transport of sophorolipids. More specifically, it relates to a Candida bombicola sophorolipid transporter protein, and the use of this transporter to modulate the secretion and/or production of glycolipids, preferably sophorolipids in organisms, preferably in fungi.Type: ApplicationFiled: December 9, 2010Publication date: December 6, 2012Inventors: Wim Soetaert, Inge Van Bogaert
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Patent number: 8323917Abstract: The present invention enables screening for compounds that inhibit the transport of GPI-anchored proteins to fungal cell walls, using a simple assay for transacylation to GlcN-PI using membrane fraction expressing GWT1 protein. New antifungal agents can be created that inhibit the synthesis of fungal cell walls and also inhibit adhesion to host cells by inhibiting the transport of GPI-anchored proteins to fungal cell walls.Type: GrantFiled: November 21, 2003Date of Patent: December 4, 2012Assignees: National Institute of Advanced Industrial Science and Technology, Eisai R&D Management Co., Ltd.Inventors: Kappei Tsukahara, Mamiko Tsuchiya, Yoshifumi Jigami, Kenichi Nakayama, Mariko Umemura, Michiyo Okamoto
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Patent number: 8288140Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: April 14, 2010Date of Patent: October 16, 2012Assignee: Novozymes, Inc.Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
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Publication number: 20120260371Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 28, 2010Publication date: October 11, 2012Applicants: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Kimberly Brown, Eric Abbate, Nikolaj Spodsberg
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Patent number: 8278088Abstract: The present invention provides novel glycosyltransferase proteins produced by ascomycetous filamentous fungi (preferably species belonging to the genus Trichoderma, more preferably Trichoderma viride), as well as genes encoding the same. Among novel enzyme proteins provided by the present invention, particularly preferred is an enzyme protein obtained from the culture supernatant of Trichoderma viride strain IAM5141. The novel enzymes of the present invention allow glycosylation of flavonoid compounds to thereby improve their water solubility.Type: GrantFiled: January 18, 2008Date of Patent: October 2, 2012Assignee: Suntory Holdings LimitedInventors: Misa Ochiai, Harukazu Fukami, Masahiro Nakao, Akio Noguchi
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Publication number: 20120245331Abstract: The present invention provides a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2 or its modified sequence and having a biotin-binding activity and replacement selected from the group consisting of: 1) replacement of the 36th serine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 2) replacement of the 80th tryptophan residue of SEQ ID NO: 2 with a hydrophilic amino acid residue; 3) replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 4) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 78th threonine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 5) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid thatType: ApplicationFiled: June 24, 2009Publication date: September 27, 2012Inventors: Yoshimitsu Takakura, Masako Tsunashima, Kozue Sofuku
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Patent number: 8268606Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: April 20, 2010Date of Patent: September 18, 2012Assignee: Novozymes A/SInventors: Alfredo Lopez de Leon, Michael Rey
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Patent number: 8242329Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: March 29, 2012Date of Patent: August 14, 2012Assignees: Novozymes, Inc., Novozymes A/SInventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Isak Jorgensen, Randall Kramer
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Patent number: 8242328Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: March 26, 2012Date of Patent: August 14, 2012Assignees: Novozymes, Inc., Novozymes A/SInventors: Junxin Duan, Lan Tang, Ye Liu, Wenping Wu, Jason Quinlan, Randall Kramer
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Publication number: 20120156173Abstract: The present disclosure relates to a novel ascomyceteous fungus, Sphaerodes mycoparasitica, for controlling plant fungal pathogens, disease symptoms, and mycotoxins in planta and ex planta. Uses, methods, compositions, sequences, and products are also disclosed herein.Type: ApplicationFiled: February 27, 2012Publication date: June 21, 2012Inventor: Vladimir Vujanovic
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Publication number: 20120142051Abstract: Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.Type: ApplicationFiled: March 2, 2010Publication date: June 7, 2012Applicant: Noda Institute for Science ResearchInventors: Masahiro Ogawa, Yasuji Koyama, Masayuki Machida
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Publication number: 20120129241Abstract: An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.Type: ApplicationFiled: October 21, 2010Publication date: May 24, 2012Applicant: ALLIANCE FOR SUSTAINABLE ENGERGY, LLCInventors: MIN ZHANG, ARJUN SINGH, PIRKKO SUOMINEN, ERIC KNOSHAUG, MARY ANN FRANDEN, ERIC JARVIS
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Patent number: 8168863Abstract: Compositions and methods for increasing the expression and/or accumulation of cellobiohydrolase enzyme in the vacuoles of plant cells are provided. The method involves targeting the enzyme to the vacuoles through the use of a barley polyamine oxidase (BPAO) vacuole sorting signal peptide. Plants transformed with an expression construct encoding the vacuole sorting signal peptide operably linked to the cellobiohydrolase enzyme direct expression of the polypeptide to the vacuoles of the plant cells. Transgenic plants, seeds, and plant tissues, and plant parts are provided. Downstream uses of transgenic plants or plant material expressing the constructs of the invention include agronomical and industrial uses, for example, human food, animal feed, biofuel, industrial alcohol, fermentation feedstocks, and the like.Type: GrantFiled: January 26, 2009Date of Patent: May 1, 2012Assignee: Syngenta Participations AGInventor: Stacy Miles
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Patent number: 8168858Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding novel delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.Type: GrantFiled: June 19, 2009Date of Patent: May 1, 2012Assignee: E. I. du Pont de Nemours and CompanyInventors: Brian McGonigle, Anthony J. Kinney, Howard Glenn Damude
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Publication number: 20120094378Abstract: The present invention is directed to nucleic acids encoding for fusion peptides comprising a fungal targeting agent and a channel-forming domain consisting essentially of amino acids 451-626 of colicin Ia, as well as vectors having the nucleic acids of the invention and host cells having the vectors. The fusion peptides of the peptides of the present invention are particularly useful for the treatment of fungal infections in a wide variety of organisms. The fusion peptides can be prepared from the nucleic acids, such as when a vector having the nucleic acid is included in a host cell.Type: ApplicationFiled: March 15, 2011Publication date: April 19, 2012Applicant: PHEROMONICIN BIOTECH, LTD.Inventor: Xiao-Qing Qiu
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Patent number: 8158395Abstract: The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: GrantFiled: June 21, 2011Date of Patent: April 17, 2012Assignee: Novozymes, Inc.Inventors: Amanda Fischer, Debbie Yaver
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Patent number: 8158404Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: GrantFiled: March 30, 2011Date of Patent: April 17, 2012Assignee: Gevo, Inc.Inventors: Doug Lies, Stephanie Porter-Scheinman, Julie Kelly, Catherine Asleson Dundon, Aristos Aristidou, Andrew Hawkins
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Patent number: 8153415Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: GrantFiled: March 31, 2011Date of Patent: April 10, 2012Assignee: Gevo, Inc.Inventors: Thomas Buelter, Andrew Hawkins, Stephanie Porter-Scheinman, Peter Meinhold, Catherine Asleson Dundon, Aristos Aristidou, Jun Urano, Doug Lies, Matthew Peters, Melissa Dey, Justas Jancauskas, Julie Kelly, Ruth Berry
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Patent number: 8153859Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.Type: GrantFiled: May 22, 2009Date of Patent: April 10, 2012Assignee: E.I. du Pont de Nemours and CompanyInventors: Knut Meyer, Howard Glenn Damude, Kevin G. Ripp, Kevin L. Stecca
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Patent number: 8148510Abstract: Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided.Type: GrantFiled: June 8, 2010Date of Patent: April 3, 2012Assignees: Pioneer Hi-Bred International, Inc., E. I. du Pont de Nemours and Company, The Regents of the University of CaliforniaInventors: Daniel J. Altier, Glen Dahlbacka, Irina Ellanskaya, Natalia Ellanskaya, legal representative, Rafael Herrmann, Jennie Hunter-Cevera, Billy F. McCutchen, James K. Presnail, Janet A. Rice, Eric Schepers, Carl R. Simmons, Tamas Torok, Nasser Yalpani
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Patent number: 8143049Abstract: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.Type: GrantFiled: August 31, 2009Date of Patent: March 27, 2012Assignee: Iogen Energy CorporationInventors: Christopher Hill, James Lavigne, Martine Whissel, John J. Tomashek
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Patent number: 8143036Abstract: Genetically modified microorganisms that produce itaconic acid at high yields and uses thereof.Type: GrantFiled: May 11, 2009Date of Patent: March 27, 2012Assignee: Industrial Technology Research InstituteInventors: James C. Liao, Pei-Ching Chang
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Patent number: 8143047Abstract: The invention relates to functional polypeptides secreted from Botryospaeria rhodina CBS 274.96.Type: GrantFiled: December 4, 2009Date of Patent: March 27, 2012Assignee: Novozymes AlsInventors: Kirk Matthew Schnorr, Lene Lange, Pernille Uldall Bolvig
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Patent number: 8143473Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia lipolytica are used to achieve the increase in seed storage lipids.Type: GrantFiled: May 23, 2008Date of Patent: March 27, 2012Assignee: E. I. du Pont de Nemours and CompanyInventors: Knut Meyer, William D. Hitz, Narendra S. Yadav, Howard G. Damude
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Publication number: 20120065106Abstract: Methods for enhancing expression levels, secretion, and purification of heterologous fusion proteins in a host cell are disclosed.Type: ApplicationFiled: December 28, 2007Publication date: March 15, 2012Applicant: LIFESENSORS, INC.Inventors: Tauseef R. Butt, Tadas Panavas, Amolkumar Karwa, Raymond J. Peroutka, Jeffrey G. Marblestone
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Publication number: 20120065251Abstract: The invention provides a nucleic acid construct comprising a promoter sequence derived from microRNA-21 (miR-21) linked to a nucleic acid sequence encoding an anti-cancer agent, an example of which is a toxin. The constructs of the invention are particularly useful for treating tumors expressing miR-21.Type: ApplicationFiled: January 19, 2010Publication date: March 15, 2012Inventors: Rina Rosin-Arbesfeld, Ella Sklan, Alona Zilberberg, Naama David
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Patent number: 8133715Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: GrantFiled: March 29, 2011Date of Patent: March 13, 2012Assignee: Gevo, Inc.Inventors: Thomas Buelter, Andrew Hawkins, Stephanie Porter-Scheinman, Peter Meinhold, Catherine Asleson Dundon, Aristos Aristidou, Jun Urano, Matthew Peters, Melissa Dey, Justas Jancauskas, Julie Kelly, Ruth Berry
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Publication number: 20120054910Abstract: Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for family members of a novel family of antipathogenic polypeptides. Polynucleotides that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the polynucleotides disclosed herein is further provided. Compositions comprising an antipathogenic polypeptide or a microorganism comprising an antipathogenic polynucleotide of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Plants, plant cells, seeds, and microorganisms comprising an antipathogenic polnucleotide or polypeptide of the invention are also disclosed.Type: ApplicationFiled: August 9, 2011Publication date: March 1, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Daniel J. Altier, Jacob T. Gilliam, Eric J. Schepers, Nasser Yalpani
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Patent number: 8110389Abstract: A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.Type: GrantFiled: July 30, 2009Date of Patent: February 7, 2012Assignee: Iogen Energy CorporationInventors: James A. Lavigne, Brian R. Scott, Martine Whissel, John J. Tomashek
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Publication number: 20120023623Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 30, 2011Publication date: January 26, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Marc D. Morant, Shamkant Patkar, Hanshu Ding
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Publication number: 20120011619Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 21, 2011Publication date: January 12, 2012Applicant: NOVOZYMES, INC.Inventors: Alfredo Lopez de Leon, Hanshu Ding, Kimberly Brown
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Patent number: 8093456Abstract: The present invention provides transgenic cover crop plants which after harvest degrade the hemicellulose and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise cellulase, hemicellulase and optionally ligninase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular, the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the hemicellulase and cellulase which then degrade the hemicellulose and cellulose of the transgenic plants to produce the fermentable sugars.Type: GrantFiled: December 4, 2007Date of Patent: January 10, 2012Assignee: Board of Trustees of Michigan State UniversityInventor: Masomeh B. Sticklen
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Patent number: 8093029Abstract: A heat-resistant bilirubin oxidase mutant is disclosed. The bilirubin oxidase mutant is obtained by deletion, replacement, addition or insertion of at least one amino acid residue of a wild type amino sequence of SEQ. ID. No. 1 of a bilirubin oxidase derived from an imperfect filamentous fungus, Myrothecium verrucaria. A biocathode including a thermo stable bilirubin oxidase mutant immobilized thereon, and its use in a biofuel cell is disclosed.Type: GrantFiled: May 19, 2009Date of Patent: January 10, 2012Assignee: Sony CorporationInventors: Hideyuki Kumita, Yuichi Tokita, Yoshio Goto
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Publication number: 20110321197Abstract: A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities selected from the group consisting of 17.6 kDa class I heat shock protein, 26.Type: ApplicationFiled: October 2, 2009Publication date: December 29, 2011Applicant: BASF Plant Science GmbHInventors: Hardy Schön, Oliver Thimm, Gerhard Ritte, Oliver Bläsing, Koen Bruynseels, Yves Hatzfeld, Valerie Frankard, Ana Isabel Sanz Molinero, Christophe Reuzeau, Steven Vandenabeele
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Publication number: 20110312047Abstract: The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: ApplicationFiled: June 21, 2011Publication date: December 22, 2011Applicant: Novozymes, Inc.Inventors: Amanda Fischer, Debbie Yaver