Extraction Processes (e.g., Solvent Extraction Process, Etc.) Patents (Class 536/25.41)
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Publication number: 20140243232Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for reducing the complexity of nucleotide sequences in a nucleic acid sample comprising the steps: hybridizing a plurality of polynucleotide constructs to at least one blocker oligonucleotide and to at least one capture oligonucleotide, wherein the plurality of polynucleotide constructs include a plurality of polynucleotides each joined to at least one nucleic acid adaptor, wherein the at least one nucleic acid adaptor can hybridize to the at least one blocker oligonucleotide, and wherein the at least one capture oligonucleotide can hybridize to at least a portion of target polynucleotides that are a sub-population of the plurality of polynucleotides, so as to produce a capture duplex.Type: ApplicationFiled: July 13, 2012Publication date: August 28, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Gavin Meredith, Christopher Clouser, Tanya Sokolsky, Kimberly Mather, Kevin McKernan, Marie Callahan, Gary Bee
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Patent number: 8816064Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.Type: GrantFiled: September 30, 2011Date of Patent: August 26, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde
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Patent number: 8816063Abstract: The present invention relates to a method for purifying a defined amount of nucleic acids from a nucleic acid-containing sample, which has at least the following steps: (a.) contacting the nucleic acid-containing sample with a defined amount of a nucleic acid binding phase with the following features: (i) the nucleic acid binding phase has nucleic acid binding ligands that have at least one protonatable group; (ii) the nucleic acid binding ligands are bound to a carrier; (iii) the nucleic acid binding phase has a surface with a low charge density, wherein the amount of nucleic acids in the sample exceeds the binding capacity of the amount of nucleic acid binding phase used; (b.) binding of the nucleic acids to the nucleic acid binding phase at a pH (binding pH) that is below the pKs value of at least one of the protonatable groups; (c.) elution of the nucleic acids at a pH that is above the binding pH, wherein a defined amount of nucleic acids is obtained.Type: GrantFiled: May 11, 2010Date of Patent: August 26, 2014Assignee: Qiagen GmbHInventors: Jan Petzel, Holger Wedler, Roland Fabis
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Publication number: 20140234942Abstract: A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.Type: ApplicationFiled: April 25, 2014Publication date: August 21, 2014Applicant: GENERAL ELECTRIC COMPANYInventors: Ernest William Kovacs, Erik Leeming Kvam, Bing Li, Frank John Mondello
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Publication number: 20140227691Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.Type: ApplicationFiled: May 16, 2011Publication date: August 14, 2014Applicant: FLUIDIGM, INC.Inventors: Andrew May, Alain Mir, Ramesh Ramakrishnan, Bernhard G. Zimmermann
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Publication number: 20140220585Abstract: Collection devices and kits for biological sample collection include a biologic sample collection device having a hydrophilic swab matrix that includes a modified polycaprolactone (PCL). Methods of production and use thereof are also described herein. The biologic sample collection devices, kits and methods described herein are used to collect a biologic sample (e.g., blood, buccal cells, etc.) and to enable extraction of nucleic acids (e.g., DNA) from that biologic sample so that the nucleic acids can be analyzed (e.g., sequencing and subsequent analyses of DNA).Type: ApplicationFiled: April 18, 2014Publication date: August 7, 2014Applicant: Diomics CorporationInventors: Jeff Morhet, Thomas Kindt, Franco Ferrari, Vasana Maneeratana, Frederic Zenhausern
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Publication number: 20140212871Abstract: Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.Type: ApplicationFiled: May 11, 2012Publication date: July 31, 2014Applicant: EXOSOME DIAGNOSTICS, INC.Inventors: Wayne Comper, Leileata M. Russo, Johan Karl Olov Skog
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Publication number: 20140194313Abstract: The present invention relates to a microfluidic device for extracting and isolating DNA from cells. The device includes a support having an inlet port for receiving a sample containing a cell, an outlet port for dispensing DNA isolated from the cell, and a microfluidic channel disposed within the support and extending from the inlet port to the outlet port. The microfluidic channel includes a micropillar array, an inflow channel disposed between the inlet port and the micropillar array, and an outflow channel disposed between the micropillar array and the outlet port. The micropillar array includes micropillars spatially configured to entrap, by size exclusion, the cell, to immobilize DNA released from the cell, and to maintain the immobilized DNA in elongated or non-elongated form when hydrodynamic force is applied to the microfluidic channel. Systems and methods of making and using the device are also provided herein.Type: ApplicationFiled: June 6, 2012Publication date: July 10, 2014Applicant: CORNELL UNIVERSITYInventors: Harold G. Craighead, Juraj Topolancik, Harvey Tian, Christopher Wallin
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Publication number: 20140194613Abstract: The presently disclosed subject matter is directed to methods of aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker in microvesicles isolated from a biological sample from the subject.Type: ApplicationFiled: July 11, 2013Publication date: July 10, 2014Applicant: THE GENERAL HOSPITAL CORPORATIONInventors: Johan Karl Olov Skog, Xandra O. Breakefield, Dennis Brown, Kevin C. Miranda, Leileata M. Russo
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Publication number: 20140179909Abstract: Various embodiments of the present disclosure generally relate to molecular biological protocols, equipment and reagents for the extraction and fractionation of DNA molecules, from whole or lysed samples, in a single flow-through device.Type: ApplicationFiled: November 30, 2011Publication date: June 26, 2014Applicant: QUANTUMDX GROUP LIMITEDInventors: Jonathan James O'Halloran, Elaine Harrington Warburton, Matthew Daniel Solomon, John Edward McCormack, Matthias Schuenemann, David James Briggs, Mindy Lee Andre
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Patent number: 8759753Abstract: A method for assaying one or more immunosuppressant drug analytes in a sample derived from whole blood comprises: (a) passing the sample dissolved in a mobile phase through a length of 30 mm or less of a stationary phase of a reversed-phase chromatographic column; (b) eluting the separated analytes from the reversed-phase chromatographic column; (c) ionizing molecules of the eluted separated analytes by a heated electrospray ionization source of a mass spectrometer so as to generate a plurality of precursor ion species; (d) isolating, for each analyte, a respective one of the precursor ion species; (e) fragmenting ions of each of the isolated precursor ion species in a fragmentation cell of the mass spectrometer so as to generate a plurality of product ions therefrom; and (f) detecting, for each analyte, the presence and quantity of a respective one of the product ion species using a detector of the mass spectrometer.Type: GrantFiled: March 14, 2013Date of Patent: June 24, 2014Assignee: Thermo Finnigan LLCInventors: Joseph M. Di Bussolo, Marta Kozak
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Publication number: 20140162347Abstract: An apparatus adapted to extract an ingredient from a fluid sample by bidirectional transferring of an aliquot of fluid having magnetic particles therein, said apparatus comprising: an open compartment operable at a constant ambient pressure; a closed compartment having a closed pressure, and an upper zone containing an air pocket adapted to generate internal positive and negative pressure; an intermediate transferring-retaining compartment adapted to temporarily retain a fraction of said aliquot of fluid; a flow barrier member adapted to inhibit a flow of said aliquot of fluid when said closed pressure is substantially equal to said ambient pressure and to allow said flow of fluid when said closed pressure is substantially different from said ambient pressure, the pressure differential controllable by a thermo member; a capturing zone adapted to allow magnetic particles in the fluid to be attracted in response to a magnetic force applied.Type: ApplicationFiled: July 5, 2013Publication date: June 12, 2014Inventor: Beno ALSPEKTOR
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Publication number: 20140141424Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.Type: ApplicationFiled: October 23, 2013Publication date: May 22, 2014Inventors: FARZAD POURAHMADI, William McMillan, Jesus Ching, Ronald Chang, Lee A. Christel, Gregory T.A. Kovacs, M. Allen Northrup, Kurt E. Petersen
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Patent number: 8729252Abstract: A method for isolating polynucleotides, such as DNA, RNA and hybrids thereof from an aqueous solution containing polynucleotides by reversibly binding the polynucleotides to silica-coated magnetic particles in the presence of a salt and non-ionic hydratable additive is disclosed. The salt and non-ionic hydratable additive concentrations are adjusted to levels that result in adhesion of the nucleic acid to the particles without degradation or precipitation of the nucleic acid.Type: GrantFiled: August 16, 2004Date of Patent: May 20, 2014Assignee: Qiagen GmbHInventors: Ralf Himmelreich, Sabine Werner
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Patent number: 8710211Abstract: Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.Type: GrantFiled: December 3, 2012Date of Patent: April 29, 2014Assignee: Handylab, Inc.Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig
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Patent number: 8703931Abstract: The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.Type: GrantFiled: November 20, 2009Date of Patent: April 22, 2014Assignee: Siemens Healthcare Diagnostics Inc.Inventors: Heike Euting, Guido Hennig, Alexandre Izmailov
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Patent number: 8691969Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.Type: GrantFiled: June 14, 2011Date of Patent: April 8, 2014Assignee: Life Technologies ASInventors: Arne Helge Deggerdal, Frank Larsen
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Publication number: 20140094597Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a solid support, preferably magnetic beads, in the presence of an ethylene glycol multimer consisting of from 2 to 70 ethylene oxide monomers, preferably tetraethylene glycol, whereby soluble nucleic acid in said sample is bound to the surface of the support, and separating said support with bound nucleic acid from the sample. Kits for performance of the invention are also provided.Type: ApplicationFiled: September 24, 2013Publication date: April 3, 2014Applicant: LIFE TECHNOLOGIES ASInventor: Erling FINNE
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Patent number: 8686129Abstract: The present invention provides a method for the isolation of biological molecules by the adsorption of the molecules onto a mineral substrate in the presence of an appropriate mixture of salts and sufolane. Preferably, the biological molecules are nucleic acids. Compositions and kits for performing the process according to the invention are also provided.Type: GrantFiled: March 20, 2007Date of Patent: April 1, 2014Assignee: Agilent Technologies, Inc.Inventors: Jeffrey C. Braman, Lee S. Basehore, Natalia Novoradovskaya
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Patent number: 8680262Abstract: A method for purifying a protected oligonucleotide comprising the steps of: a) providing a solution of the protected oligonucleotide in at least one solvent A having a boiling point below the boiling point of a solvent B, heating the solution at a temperature of at least 30° C. and below the boiling point of the at least solvent A, adding solvent B until precipitation of a material is visible in the solution, said solvent B being an alcohol having 1 to 6 C-atoms or a diol having 2 to 6 C-atoms, allowing the solution to cool down under stirring until formation of a supernatant and a residue, removing the supernatant or b) providing solvent B, said solvent B being an alcohol having 1 to 6 C-atoms or a diol having 2 to 6 C-atoms, heating solvent B at a temperature above 30° C.Type: GrantFiled: March 15, 2012Date of Patent: March 25, 2014Assignee: Girindus AGInventors: Meinhof Lange, Olaf Groessel, Fritz Link, Andreas Schoenberger, Andreas Hohlfeld
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Patent number: 8664378Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.Type: GrantFiled: September 30, 2011Date of Patent: March 4, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde
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Patent number: 8664377Abstract: A method and apparatus for efficiently isolating nucleic acids from a nucleic acid-containing sample.Type: GrantFiled: August 9, 2010Date of Patent: March 4, 2014Assignee: Samsung Electronics Co., Ltd.Inventors: Kyu-youn Hwang, Joon-ho Kim, Kak Namkoong
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Publication number: 20140051847Abstract: The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsulfate solution to the lysed suspended sample, carrying out solvent extraction and separation to obtain an aqueous phase, reacting the aqueous phase with solvents to generate an insoluble precipitate containing nucleic acid, and isolating the nucleic acid therefrom, thereby releasing high molecular weight nucleic acid pellets from the cells.Type: ApplicationFiled: September 11, 2009Publication date: February 20, 2014Applicant: UNIVERSITI PUTRA MALAYSIAInventors: Mohd Ali Hassan, Meisam Tabatabaei, Mohd Rafein Zakaria, Abdul Raha Rahim, Ander-Denis G. Wright, Yoshihito Shirai, Norhani Abdullah, Mehdi Shamsara, Kenji Sakai
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Publication number: 20140046050Abstract: The disclosure provides a composition and method for isolating nucleic acids, in which the composition includes at least one halocarbon, at least one salt and at least one surfactant. Mixing the composition and a biosample to form a homogenized solution, nucleic acids in the solution can be easily isolated with a simple treatment and good yield.Type: ApplicationFiled: August 9, 2013Publication date: February 13, 2014Applicant: Industrial Technology Research InstituteInventors: Yi-Chang CHEN, Jane S-C TSAI
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Publication number: 20140039176Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.Type: ApplicationFiled: June 28, 2013Publication date: February 6, 2014Inventors: Maxim BREVNOV, Hemant PAWAR, Manohar FURTADO, Jaiprakash SHEWALE
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Publication number: 20140039177Abstract: A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.Type: ApplicationFiled: August 30, 2012Publication date: February 6, 2014Applicant: GENERAL ELECTRIC COMPANYInventors: John Richard Nelson, Li Zhu, Erin Jean Finehout, Xiaohui Chen, Kashan Ali Shaikh, Christopher Michael Puleo, Patrick McCoy Spooner
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Patent number: 8633032Abstract: A nucleic acid extraction kit, which enables the nucleic acid extraction operation to be accomplished safely without causing contamination, and in which the complex preparation of reagents and the disposal treatments that are performed before and after the nucleic acid extraction operation can be performed rapidly and simply, with the extraction performed in an automated manner. The nucleic acid extraction kit includes: a container including reagent wells that each store at least a reagent, a sample well into which a biological sample is introduced, a waste liquid well, and a collection well in which an extracted nucleic acid is collected, and an extraction filter cartridge equipped with an extraction filter for separating and extracting a nucleic acid from the biological sample, wherein the extraction filter cartridge is formed in a manner that enables the extraction filter cartridge to be supported on the waste liquid well and the collection well.Type: GrantFiled: February 19, 2013Date of Patent: January 21, 2014Assignee: Toppan Printing Co., Ltd.Inventors: Shuichi Akashi, Ryoichi Asai
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Publication number: 20140018529Abstract: A device was developed for use in nucleic acid isolation that includes reversibly connected upper and lower chambers, and contains a fibrous nucleic acid binding surface which can expand in the chambers when wetted. The device may be used in a rapid nucleic acid isolation process without clogging while accommodating complex samples.Type: ApplicationFiled: July 12, 2012Publication date: January 16, 2014Applicant: E I Du Pont De Nemours And CompanyInventor: MICHAEL P. PERRY
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Patent number: 8629264Abstract: A system, method, and kit for extracting nucleic acid from a sample containing nucleic acid uses an extraction device with an elongate channel. Fluids are provided to the channel via gravity feed to the inlet port. The flow rate and other flow behavior may be controlled with a siphon provided at the outlet port.Type: GrantFiled: May 18, 2012Date of Patent: January 14, 2014Assignee: Blood Cell Storage, Inc.Inventors: Michael W. Reed, Daniel P. Gestwick
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Patent number: 8624020Abstract: The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples, and biological materials, using a wash buffer comprising an alcohol having 1 to 3 carbon atoms and at least one further solvent selected from the group consisting of alkane diols and alkane triols having 2 to 6 carbon atoms, monocarboxylic acid esters and dicarboxylic acid diesters having 2 to 6 carbon atoms in the acidic component and 1 to 4 carbon atoms in the alcoholic component; (poly)ethylene glycols and ether derivatives and ester derivatives thereof, and poly(4-styrene sulfonic acid-co-maleic acid) sodium salt solution. The present invention further relates to a kit for carrying out the method of the invention.Type: GrantFiled: September 2, 2009Date of Patent: January 7, 2014Assignee: Qiagen GmbHInventors: Ralf Himmelreich, Sabine Werner
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Publication number: 20130338245Abstract: A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.Type: ApplicationFiled: May 20, 2013Publication date: December 19, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: Matthew BAKER
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Publication number: 20130338350Abstract: The current disclosure provides methods and kits for isolating nucleic acid from an environmental sample. The current methods and compositions further provide methods for isolating nucleic acids by reducing adsorption of nucleic acids by charged ions and particles within an environmental sample. The methods of the current disclosure provide methods for isolating nucleic acids by releasing adsorbed nucleic acids from charged particles during the nucleic acid isolation process. The current disclosure facilitates the isolation of nucleic acids of sufficient quality and quantity to enable one of ordinary skill in the art to utilize or analyze the isolated nucleic acids for a wide variety of applications including, sequencing or species population analysis.Type: ApplicationFiled: June 14, 2013Publication date: December 19, 2013Inventors: Richard Ashley Hurt, JR., Dwayne A. Elias
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Publication number: 20130331560Abstract: A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA. The process includes the steps of alkaline lysis and a neutralization. For separating the lysate and the precipitate, the mixture is allowed to gently flow downward through a clarification reactor that is partially filled, in its lower part, with retention material like glass beads, whereby the precipitate is retained on top of and within the retention. In a preferred embodiment of the lysis step, cell suspension and alkaline lysis solution flow through a lysis reactor that is filled with particulate material like glass beads. The process can be run continuously and fully automated.Type: ApplicationFiled: June 24, 2013Publication date: December 12, 2013Applicant: BOEHRINGER INGELHEIM INTERNATIONAL GMBHInventors: Jochen URTHALER, Roman NECINA, Christine ASCHER, Helga WOEHRER
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Patent number: 8592200Abstract: The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample.Type: GrantFiled: December 21, 2012Date of Patent: November 26, 2013Assignee: University of South FloridaInventor: Matt Ewert
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Publication number: 20130303746Abstract: DNA is extracted from epithelial cells and other cells without cell walls, by use of a DNA extraction buffer that contains a C1-C4 alcohol, a cell lysis detergent, and a buffering agent maintained at a slightly basic pH. The cells are immersed in the buffer and gently agitated at ambient temperature, and DNA extraction and precipitation occur in thirty minutes or less, and often in five minutes or less.Type: ApplicationFiled: May 8, 2013Publication date: November 14, 2013Applicant: Bio-Rad Laboratories, Inc.Inventors: Bryony Ruegg, Ingrid Miller
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Publication number: 20130295652Abstract: Systems and methods for isolating samples are provided. The system comprises a first membrane and a second membrane disposed within an enclosure. First and second reservoirs can also be disposed within the enclosure and adapted to contain one or more reagents therein. A first valve can be disposed within the enclosure and in fluid communication with the first reservoir, the second reservoir, or both. The first valve can also be in fluid communication with the first or second membranes or both. The first valve can be adapted to selectively regulate the flow of the reagents from the first reservoir, through at least one of the first and second membranes, and into the second reservoir.Type: ApplicationFiled: May 1, 2012Publication date: November 7, 2013Inventors: Ye Zhang, Honglu Wu
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Publication number: 20130295574Abstract: A method for extracting nucleic acids from a biological sample by isolating nucleic acid-containing particles from the biological sample by one or more centrifugation procedures, performing one or more steps to mitigate adverse factors that prevent or might prevent high quality nucleic acid extraction, and extracting nucleic acids from the isolated particles. The centrifugation procedures are performed at a speed not exceeding about 200,000 g. The extracted nucleic acids contain both 18S and 28S rRNA.Type: ApplicationFiled: November 10, 2011Publication date: November 7, 2013Applicant: EXOSOME DIAGNOSTICS, INC.Inventors: Johan K. Skog, Leileata M. Russo
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Publication number: 20130288342Abstract: The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample.Type: ApplicationFiled: December 21, 2012Publication date: October 31, 2013Applicant: University of South FloridaInventor: University of South Florida
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Publication number: 20130280787Abstract: The present invention relates to a method for extracting nucleic acids from a wax-embedded sample, the use of particular solvents for removing wax from a wax-embedded sample in a method for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample as well as to a kit for extracting, isolating and/or purifying nucleic acids from a wax-embedded sample.Type: ApplicationFiled: June 14, 2011Publication date: October 24, 2013Applicant: QIAGEN GmbHInventors: Markus Mueller, Thorsten Singer, Esther Rosenberg, Joerg Hucklenbroich
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Publication number: 20130273640Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.Type: ApplicationFiled: April 16, 2013Publication date: October 17, 2013Applicant: Biological Dynamics, Inc.Inventors: Rajaram KRISHNAN, David CHARLOT, Eugene TU, James MCCANNA, Lucas KUMOSA, Paul SWANSON, Robert TURNER, Kai YANG, Irina DOBROVOLSKAYA, David LIU
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Patent number: 8536322Abstract: The invention includes a method for separating polynucleotides, such as DNA, RNA and PNA, from a solution containing polynucleotides by reversibly and non-specifically binding the polynucleotides to a solid surface having a functional amide group-coated surface. The materials containing a solid surface can be in the form of microparticles, fibers, beads, membranes, test tubes, pipette tips or microwells and can further comprise a magnetic core portion. The pH, salt and concentration of crowding reagent, such as polyethylene glycol or alcohol, of the solution is adjusted to levels which result in nucleic acid binding to the solid surface. The magnetic microparticles with bound polynucleotides are separated from the solution under mild alkaline conditions and the nucleic acids are eluted from the magnetic microparticles. Solutions having different nucleic acid concentrations can be normalized by restricting the availability of the solid phase surface.Type: GrantFiled: October 18, 2010Date of Patent: September 17, 2013Inventor: Zhiqiang Han
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Patent number: 8519119Abstract: A formulation containing guanidine thiocyanate or guanidine hydrochloride together with acetamide, one or more acetamide derivatives, or a combination of acetamide and one or more acetamide derivatives is provided with methods to use the formulation to purify one or more nucleic acids contained in a medium.Type: GrantFiled: September 14, 2011Date of Patent: August 27, 2013Assignee: Promega CorporationInventor: Rex M. Bitner
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Patent number: 8513405Abstract: Disclosed are a chaotropic agent; a reagent including a chaotropic agent and a lithium salt; a reagent kit including a chaotropic agent; a chaotropic agent, a reagent, a reagent kit, and a method for isolating a nucleic acid by use of a magnetic cellulose material; a method for binding a nucleic acid to a magnetic cellulose material; a method for isolating a nucleic acid; and a method for purifying a chromosome DNA. It is required that each of the chaotropic agents, the reagents, and the reagent kits works with at least one solid-phase, magnetic cellulose-containing carrier to isolate a nucleic acid from non-nucleic acid substances. In addition, each chaotropic agent includes an alcohol substance and a substrate solution for adjusting the alcohol substance to an appropriate concentration and thereby promoting binding of the nucleic acid in a sample to the magnetic cellulose.Type: GrantFiled: April 2, 2008Date of Patent: August 20, 2013Assignee: RBC Bioscience Corp.Inventors: Pei-Shin Jiang, Yu-Ting Su, Kun-Chan Wu, Hui-Ju Cho, Wen-Hsun Kuo, Yuh-Jiuan Lin, Yi-Ling Li, Cheng-Chun Kuan
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Publication number: 20130189700Abstract: Methods of processing an emulsion of aqueous droplets containing nucleic acid. The methods may include breakage of the emulsion with a destabilizing fluid including a halogen-substituted hydrocarbon.Type: ApplicationFiled: July 25, 2012Publication date: July 25, 2013Applicant: BIO-RAD LABORATORIES, INC.Inventors: Austin P. So, Svilen S. Tzonev, Serge Saxonov, Benjamin J. Hindson, Michael Y. Lucero
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Publication number: 20130171697Abstract: A microfluidic device for controlling a flow of a fluid using centrifugal and rotational force based on a rotatable disc-type body, a method of separating a target material or performing emulsion nucleic acid amplification using the microfluidic device.Type: ApplicationFiled: October 26, 2012Publication date: July 4, 2013Applicant: Samsung Electronics Co., Ltd.Inventor: Samsung Electronics Co., Ltd.
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Publication number: 20130165643Abstract: A nucleic acid extraction kit, which enables the nucleic acid extraction operation to be accomplished safely without causing contamination, and in which the complex preparation of reagents and the disposal treatments that are performed before and after the nucleic acid extraction operation can be performed rapidly and simply, with the extraction performed in an automated manner. The nucleic acid extraction kit includes: a container including reagent wells that each store at least a reagent, a sample well into which a biological sample is introduced, a waste liquid well, and a collection well in which an extracted nucleic acid is collected, and an extraction filter cartridge equipped with an extraction filter for separating and extracting a nucleic acid from the biological sample, wherein the extraction filter cartridge is formed in a manner that enables the extraction filter cartridge to be supported on the waste liquid well and the collection well.Type: ApplicationFiled: February 19, 2013Publication date: June 27, 2013Applicant: TOPPAN PRINTING CO., LTD.Inventor: TOPPAN PRINTING CO., LTD.
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Publication number: 20130165632Abstract: Provided is a novel method for separating water-soluble biological substances. A separating agent is composed by bonding a polysaccharide such as cellulose or amylose to the surface of a carrier by chemical bonding, and water-soluble biological substances are separated from a mixture of two or more types of water-soluble biological substances by chromatography using the separating agent.Type: ApplicationFiled: September 9, 2011Publication date: June 27, 2013Inventors: Atsushi Ohnishi, Ryota Hamasaki
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Publication number: 20130158240Abstract: A method is provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The method includes the steps of capturing the fraction-bound solid phase substrate and bringing an isolation buffer and the fraction-bound solid phase substrate into contact to purify the captured fraction-bound solid phase substrate.Type: ApplicationFiled: December 15, 2011Publication date: June 20, 2013Inventors: David Beebe, Lindsay Strolman, Scott M. Berry
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Patent number: 8465966Abstract: The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample.Type: GrantFiled: May 8, 2008Date of Patent: June 18, 2013Assignee: University of South FloridaInventor: Matt Ewert
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Publication number: 20130144049Abstract: A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises: (a) phenol in an amount of more than 50% by volume based on the total amount of the solution; (b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution; (c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution; (d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and (e) a buffer for maintaining the pH of the solution at 4 to 6.Type: ApplicationFiled: August 17, 2011Publication date: June 6, 2013Applicant: Toray Industries, Inc.Inventors: Makiko Yoshimoto, Hideo Akiyama, Hitoshi Nobumasa