Abstract: The invention relates to a method for recovering at least short RNA, having at least the following steps: a) making available a biological solution containing at least short RNA as well as proteins and/or long nucleic acids (long RNA and DNA); b) removing the proteins and the long nucleic acids from the solution, at least the proteins being precipitated; c) adsorbing the short RNA onto a solid (first) carrier after precipitation of the proteins; d) recovering the short RNA by desorption from the carrier. The invention further includes a kit for carrying out the method.

Abstract: Methods using two to (n) purification columns to separate full length 5?-DMT-on oligonucleotides with size ranging from 40 to 180-mers from short length 5?-DMT-on oligonucleotides. Two of the said methods require using some columns sequentially with the collection and reprocessing of an intermediate fraction and are used for oligonucleotides with length ranging from 70 to 180-mers. A third method is carried out with columns stacked and used in series and is best used to purify oligonucleotides with length ranging from 40 to 80-mers. In the presence of a high ionic strength buffer, the short length DMT-on oligonucleotides bind to the top stacked columns while the less hydrophobic contaminant or DMT-off failures do not bind and/or are being washed off. In a stacked configuration, the full length DMT-on oligonucleotides are retained by the bottom column while in a 15 sequential configuration, full length DMT-on oligonucleotides are collected and reprocessed.

Abstract: The present invention provides an attachment or extension for pipettes and pipette tips. The attachment comprises separation materials which are preferably suitable for isolating nucleic acids from liquids. The separation materials are present in the form of a filter disc in a holder which only slightly increases the pipette volume and thus generates little dead volume.

Abstract: A method and apparatus for exposing a sample, including a liquid and another material, to sonic energy to produce a desired result such as, suspending a material support in the liquid. The material support may be a bead or other particle with at least one surface feature to which the material may bind. Material in the liquid may attach to the material support, such as by specific or non-specific binding, entrapment or other, so as to facilitate separation of the material from the liquid. Separation of the material supports from the liquid and other unbound material may be done by allowing the material supports to settle out, e.g., under the force of gravity and/or as assisted by centrifugation, by applying a magnetic field in case the supports or material bound to the supports are movable by way of a magnetic field, or other techniques.

Abstract: Disclosed are a method for isolating a nucleic acid using particulate matter and a composition therefor. The method comprises essentially the steps of adding a lysis buffer and a neutralization buffer to a biological sample sequentially, and centrifuging cell lysates for at least 10 minutes to separate a solution comprising the nucleic acid and insoluble aggregate comprising denatured protein aggregate and cell debris. The particulate matter is added to and participates in co-aggregation and co-precipitation of denatured protein aggregate and cell debris, and co-aggregates and co-precipitates denatured protein aggregate and cell debris within a much shortened time, thereby to shorten the time and to increase the yield for the isolation of a nucleic acid, compared with conventional methods.

Abstract: Reagents, methods and kits for the purification of RNA from biological materials are provided.

Abstract: Embodiments of the present invention provide methods and kits for purifying nucleic acids. In particular, embodiments of the present invention provide methods and kits for purifying nucleic acids through the use of magnetic particles in binding buffers.

Abstract: Some aspects relate to methods for genetic analysis of selected cells from within a heterogeneous population of cells. The population of cells first can be partitioned. Selected cells are identified by imaging, and then specifically targeted and lysed by irradiation with an energy beam, resulting in specific release of their cellular contents into the culture medium. The culture medium then can be sampled and assayed for the desired nucleic acids.

Abstract: The present invention describes isolation of plasmid DNA from bacteria. The addition of dyes to the alkaline lysis based purification buffers (P1, P2, and P3) allows for improved visual monitoring of the steps of preparing a bacterial lysate filtrate coupled to filtration or spin-column chromatography. The method comprises the suspending of the bacterial cells with buffer P1 (suspension is red/pink); lysing the bacteria with buffer P2 (suspension goes from red/purple color to translucent/purple); precipitating cellular debris with buffer P3 (solution becomes yellow with debris suspension); centrifuging or filtering to product a lysate filtrate; binding the lysate filtrate to a DNA binding matrix; washing; and isolating the plasmid via chromatography. The yield and quality of plasmid DNA is improved due to more consistent lysis. Errors in buffer addition are reduced by visualizing the color as buffers are added and also of changes in color of the preparation at each step.

Abstract: New processes and equipment to isolate and purify nucleic acids on surfaces are provided. The invention focuses on processes which use surfaces, for example, porous membranes, on which the nucleic acids are immobilized in a simple manner from the sample containing the nucleic acids and can be released again by way of simple procedural steps, whereby the simple performance of the process according to the invention makes it possible to perform the processes specifically in a fully automatic manner. An additional aspect of the present invention focuses on binding the nucleic acids to an immobile phase, especially to a membrane, in such a way and manner, that they can be released without difficulty during an additional reaction stage from this phase and, if desired, can be used in other applications, such as restriction digestion, RT, PCR or RT-PCR, or in any of the suitable analyses or enzyme reactions mentioned in the disclosure.

Abstract: A method for preparing a sample by utilizing a mechanical force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range.

Abstract: The invention relates, for example, to methods for preparing a plant or animal sample for processing, i.e. for example for the isolation of nucleic acids or proteins from the sample, as well as to a pulverizer.

Abstract: A system and method for the automated extraction of nucleic acids from nucleic acids containing samples are disclosed. In the system, reagents provided in cavities of a reagent plate are transferred to samples accommodated in a process plate using a pipetting device comprising pipettes provided with disposable pipette tips for pipetting of the reagents to obtain sample-reagent mixtures, the sample-reagent mixtures accommodated in the process plate are incubated by an incubating device to release the nucleic acids; the released nucleic acids accommodated in the process plate are separated by a separating device and released to obtain extracted nucleic acids containing fluids; the extracted nucleic acids containing fluids are transferred to cavities of an output plate by disposable pipette tips; wherein each of the samples is assigned one-to-one to a cavity of the reagent plate, at least one of the disposable pipette tips and a cavity of the output plate.

Abstract: The invention provides a method useful for detection of genetic disorders. The method comprises determining the sequence of alleles of a locus of interest, and quantitating a ratio for the alleles at the locus of interest, wherein the ratio indicates the presence or absence of a chromosomal abnormality. The present invention also provides a non-invasive method for the detection of chromosomal abnormalities in a fetus. The invention is especially useful as a non-invasive method for determining the sequence of fetal DNA. The invention further provides methods of isolation of free DNA from a sample.

Abstract: A system for processing a target material includes; a cartridge which stores a material which reacts with the target material, and may include at least one chamber and at least one valve connected to the at least one chamber, a first module which may be loaded with the at least one cartridge and may rotate, a second module which may selectively open or close the at least one valve, a third module which may selectively control the temperature of the at least one chamber, and a control module which may control the first module, the second module and the third module.

Abstract: This document provides methods and materials involved in collecting and processing complex macromolecular mixtures (e.g., stool samples). For example, stool collection devices, buffers for stabilizing nucleic acid and polypeptides present in stool, and kits for using sequence-specific capture probes (e.g., nucleic acid sequences designed to hybridize with particular target nucleic acids) to capture target nucleic acids directly from complex macromolecular mixtures (e.g., stool samples) without the need to perform prior steps to enrich, isolate, or purify the nucleic acid component are provided.

Abstract: The present invention relates to a process for the purification of biomolecules, in particular of nucleic acids, such as DNA and RNA molecules.

Abstract: A phenol-free method of isolating DNA from biological material includes homogenizing a biological material with a homogenization buffer to form a homogenate. Proteins and non-DNA organic molecules are extracted from the homogenate by mixing a first extraction buffer and a second extraction buffer with the homogenate. The first extraction buffer includes chloroform and an alcohol and the second extraction buffer includes a non-ionic protein solubilizer and an alcohol. DNA is precipitated from the mixture of homogenate, first extraction buffer and second extraction buffer and the DNA is recovered by sedimentation.

Abstract: The present invention relates to a cartridge which can be positioned inside an air collection means and receive a means for recovering nucleic acids, said cartridge being substantially cylindrical and comprising a microorganism retaining zone, said retaining zone comprising microorganism lysis means. The invention also relates to a device for collecting microorganisms contained in the air and a device for microorganism lysis.

Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

Abstract: The application describes a method for the preparative purification of RNA, which method is distinguished in that the RNA is purified by means of HPLC using a porous reversed phase as the stationary phase. The use of the porous reversed phase in this HPLC method is also described.

Abstract: An apparatus for purifying nucleic acids by negative chromatography is disclosed, which comprises a hollow body with an opening, respectively, at the top and at the bottom end, said hollow body containing a stationary solid phase, characterized in that the stationary phase contains at least 2 different chromatography resins, such as size e.g. exclusion gel filtration materials (SEC materials), as well as a method for purifying nucleic acids and the use of the apparatus.

Abstract: Methods for processing microparticles involve providing a composition comprising a plurality of solid microparticles and at least one non-volatile material, providing a non-solvent, and exposing the composition to the non-solvent to remove at least a portion of the non-volatile material from the composition while retaining at least the microparticles.

Abstract: The invention relates to a process for selective, reversible adsorption of nucleic acids to a support material, which process is characterized in that (a) a nucleic acid-containing mixture having a cosmotropic salt content of at least 1 mole is contacted with said support material, whereupon the nucleic acid(s) is (are) adsorbed from said nucleic acid-containing mixture to said support material, and (b) the nucleic acid(s) bound to the support material is (are) detached from the support material by means of an aqueous solution having a salt content of no more than 1 mole.

Abstract: Embodiments of the present techniques provide systems and methods for isolating particular classes of biological molecules, for example, proteins or nucleic acids, from mixtures of biological components. The methods use solutions that react with the biological molecules to enhance their adsorption by substrates, allowing contaminants to be washed away from the targeted molecules. Embodiments include automated systems that can be used to implement the technique with no or minimal intervention. Other embodiments include separation column technologies that may be used in the techniques.

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

Abstract: The present invention is a method of extracting infectious pathogens from a volume of blood including the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The fibrin lysis reagent is preferably composed of plasminogen and streptokinase frozen in coincident relation until the fibrin lysis reagent is needed whereby streptokinase enzymatically reacts with plasminogen to form plasmin upon thawing. The plasminogen is suspended in an aqueous salt solution prior to freezing including NaCl and Na3PO4.

Abstract: Embodiments relate to methods and formulations of buffers used for isolating, purifying, and recovering long-chain and short-chain nucleic acids. The areas of application of the inventive method include all laboratories engaged in isolating nucleic acids. In one embodiment a solution containing a nucleic acid is prepared with additives containing monovalent and multivalent cations and, optionally, an alcohol and/or additional additives. The solution is contacted with a solid phase, the solid phase is optionally washed, and the nucleic acid is removed. The solution may contain multivalent and/or monovalent cations and may contain an alcohol. The solution in certain embodiments has a pH between 7 and 10. Ammonium chloride, sodium chloride and/or potassium chloride may be used as monovalent salt components. Magnesium chloride, calcium chloride, zinc chloride and/or manganese chloride may be used as multivalent salt components.

Abstract: The present invention relates to a method of isolating a nucleic acid molecule form a biological sample. More particularly, the present invention relates to a method of isolating ribonucleic acid molecule from a biological sample. The method of the present invention is useful in a range of applications including, but not limited to, diagnostic applications and research and development applications, to the extent that the isolation of nucleic acid molecules, and in particular ribonucleic acid molecules, is required. Most particularly, the method of the present invention provides for the isolation of ribonucleic acid molecules which are suitable for analysis by reverse transcriptase-PCR.

Abstract: Methods for cell lysis and purification of biological materials, involving subjecting a sample to high pressure. Also featured is an apparatus for practicing the methods.

Abstract: The invention relates to methods for separating or purifying biopolymer conjugated molecules from unconjugated molecules. In particular, methods are described for purifying a PEGylated protein or oligonucleotide from an unPEGylated protein or oligonucleotide, respectively. The methods are quick and efficient separation methods because they do not require gradient chromatography, fractionation of an eluent or analysis of the eluted fractions. Further, the methods increase yield and purity of the biopolymer conjugated molecule.

Abstract: A method for separating and purifying nucleic acid, the method comprising: (1) a step of contacting a sample solution containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase; (2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and (3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase, wherein the sample solution is prepared by including a step of removing a precipitate component, and adding a surfactant and a water-soluble organic solvent to a supernatant solution of the precipitate.

Abstract: Parallel isolation of a double-stranded nucleic acid and a single-stranded nucleic acid is possible from a sample that contains these acids, without separating the acids, by mixing the sample with a lysis buffer having high salt concentration or low salt concentration, or having a proteolytic enzyme. The sample that contains nucleic acid before its lysis, or the sample that has already been lysed or homogenized, is adjusted with a binding buffer in such a manner that the total nucleic acid is adsorbed onto a solid carrier. The binding buffer contains at least one non-ionic detergent in a high concentration. With the exception of the detergent, the sample contains no other non-acidic organic component miscible in water. The carrier with the adsorbed total nucleic acid is removed. The adsorbed total nucleic acid is washed and eluted.

Abstract: Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.

Abstract: Devices, processes, and kits for the extraction of nucleic acids from biological samples are disclosed. The devices comprise a first port, a second port, and a binding chamber intermediate and in fluid communication with the first port and the second port. The binding chamber comprises an unmodified flat glass surface effective for binding a heterogeneous population of nucleic acids. The first port, second port, and binding chamber define a continuous fluid pathway that is essentially free of nucleic acid-specific binding sites.

Abstract: The present invention relates to a method for separating a material that has affinity to an antibody by using a protein-antibody conjugate with modified partition characteristics, more precisely a method for affinity separation to separate a material specifically binds to an antibody, in which an antibody is conjugated to a protein to modify partition characteristics of the protein-antibody conjugate. The method of the present invention can be effectively and widely used as a safe and efficient separation method for biomolecules since it takes advantages of safe aqueous two-phase extraction system and high selective molecular specific conjugation.

Abstract: The invention relates to a device and a method for cleaning sample material, in particular nucleic acids. The cleaning process takes place by means of centrifugal microfluidics and the invention provides a simple compact construction and a simple procedure. If necessary, the sample material is easily mixed with a solvent buffer and proteases in the device. The cleaned sample material is in particular transferred directly to a container. Separate containers for the removal of excess liquid are not required.

Abstract: The invention relates to methods of separating substances from liquid media and to separating agents suitable therefor.

Abstract: The invention relates to a method for isolating and/or identifying known or unknown, nucleic acid sequences (target sequences) which are marked, optionally, with reporter groups, by base specific hybridisation with, essentially, complementary sequences, which belong to a library or sequences. The sample sequences are characterised in that they support, on one of the termini thereof (5?-ends or 3?-ends, preferably on 5?-ends), a single double or multi-chained lipid part, which spreads in a monomolecular layer on a liquid gas or liquid-liquid phase boundary layer. The invention also relates to a system for detecting or isolating nucleic acids.

Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be coupled with a further step to isolate RNA from the same sample.

Abstract: The present invention describes a process to produce a composition containing 5?-ribonucleotides wherein a microorganism is subjected to autolysis under conditions at which a substantial part of the RNA remains in a form degradable into 5?-ribonucleotides and at which a substantial part of the RNA remains associated with the cell wall fraction. Said cell wall fraction is recovered by a solid/liquid separation method and the RNA associated with said wall fraction is converted into 5?-ribonucleotides. The present invention also describes compositions containing 5?-ribonucleotides and their use in food or feed.

Abstract: The present invention provides a solid support for performing steps of isolation of cell or extraction and purification of nucleic acid, safely, easily, efficiently, and with high yield in the genetic test for investigating the presence of pathogenic bacterial infection. A solid support for binding with cell as an embodiment of the above-described solid support, comprises a polypeptide having capability of binding with mycolic acid-containing glycolipid which is immobilized on the surface of a carrier. In addition, a solid support for binding with nucleic acid as another embodiment of the above-described solid support, comprises a polypeptide having capability of binding with nucleic acid which is immobilized on the surface of a carrier.

Abstract: The invention provides methods and compositions, e.g., kits, for removing contaminants from nucleic acids in a sample, e.g., environmental or biological samples such as soil, food, plant, animal, microorganism or water samples. The invention provides methods and compositions for isolating nucleic acids from environmental and biological samples in a scaleable process free of contaminating substances that inhibit PCR and other downstream applications. Exemplary sample types include soil, water, plant and food. The methods and compositions of the invention can be used for isolating and/or detecting nucleic acids from prokaryotic and eukaryotic organisms and for detecting multiple types of organisms in a sample. Thus, the methods and compositions of the invention are useful for detecting organisms pertaining to agriculture, forensics biology and/or combating bioterrorism.

Abstract: Provided is a method for obtaining DNA from a plant by collecting the root border cells from a growing root and extracting DNA from the root border cells. Preferably, the root border cells are contained in the root exudate of the growing root, which is growing in a medium, for example, water, tissue culture medium, or soil. Suitably, the root is part of a germinating seed, or the root of a seedling, or the adventitious root of a tissue culture plant or plant part.

Abstract: Inkjet printhead solvents and methods of forming an addressable nucleotide array are disclosed.

Abstract: Disclosed are methods permitting colonies of nucleic acids produced by amplifying them in an immobilized medium to be visualized with a homogeneous fluorescence detection system which is introduced into the medium before amplification. No post-amplification steps, such as opening the gel, blotting, hybridization, and removal of unbound substrates, dyes, or probes are needed, and the growing molecular colonies can be monitored in real time. Also disclosed are methods for quantitative in situ assays of nucleic acids contained in individual cells or in tissue fragments. The methods are conveniently executed using a disposable transparent chamber containing dry matrix of the medium and equipped by inlet and outlet ports that serve for filling the chamber with a solution comprising components of amplification and detection systems, said solution being absorbed by said matrix to swell all over the inner volume of said chamber.

Abstract: The present invention relates to methods for the extraction of long fragment RNA from fixed tissue specimens. In particular, the present invention relates to methods for the extraction of RNA from formalin-fixed paraffin-embedded tissue specimens for use in biologic applications, including assays based on oligonucleotide hybridization.

Abstract: Method for collecting cellular material of particular cells present in a liquid includes: a step (210) of introducing the liquid into a compartment via an upper opening of the compartment said compartment having a lower opening, a filter being positioned between the two openings which has micropores of a diameter intermediate between that of said particular cells and that of other cells, a filtering step (215) during which most of the liquid and most of the other cells pass through the filter, a step (230) of lysing the cells retained or: the filter and a step (245, 230) of collecting cellular material from the lysed cells, on the filter.

Abstract: A reaction unit containing a bottom part and a top part for pipetting a liquid, wherein the bottom part contains a reaction cavity with a permeable filter grid insert and the top part contains a reaction cavity which can be fixed on said bottom part and contains a cavity or covering for receiving a magnet.

Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a solid support, preferably magnetic beads, in the presence of an ethylene glycol multimer consisting of from 2 to 70 ethylene oxide monomers, preferably tetraethylene glycol, whereby soluble nucleic acid in said sample is bound to the surface of the support, and separating said support with bound nucleic acid from the sample. Kits for performance of the invention are also provided.