Labelled (e.g., Tagged With Radioactive Tracer, Fluorescent Marker, Intercalator, Etc.) Patents (Class 536/26.6)
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Patent number: 8742084Abstract: This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. The invention also relates to targets isolated with conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. The invention further relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and individual substances in a complex background of substances.Type: GrantFiled: September 14, 2012Date of Patent: June 3, 2014Assignee: The Trustees of Boston UniversityInventors: Kenneth J. Rothschild, Sanjay M. Sonar, Jerzy Olejnik
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Patent number: 8716457Abstract: The present invention provides modified nucleobase compounds, modified nucleic acid mimetic compounds and various uses thereof. In addition, the invention provides methods for nucleobase characterisation, SNP characterisation and nucleic acid sequencing.Type: GrantFiled: September 19, 2008Date of Patent: May 6, 2014Assignee: The University Court of the University of EdinburghInventors: Mark Bradley, Juan J. Diaz-Mochon
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Patent number: 8709722Abstract: There is provided a method for detecting binding of a DNA-binding protein to a target recognition sequence. The method comprises mixing in a reaction buffer a first set of metal nanoparticles, a second set of metal nanoparticles and a DNA-binding protein to form a mixture, and detecting the aggregation state of the mixture of metal nanoparticles. Each set of metal nanoparticles has a conjugated double-stranded DNA molecule having a single-stranded overhang at one end. The single-stranded overhangs of each set of DNA-conjugated metal nanoparticles are complementary to each other such that annealing of the complementary overhangs results in formation of the target recognition sequence that specifically binds the DNA-binding protein. The reaction buffer comprises an ionic species in a concentration sufficient to result in aggregation of the metal nanoparticles upon annealing of the first and second single-stranded overhang.Type: GrantFiled: August 24, 2012Date of Patent: April 29, 2014Assignee: Agency for Science, Technology and ResearchInventors: Yen Nee Tan, Xiaodi Su
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Patent number: 8709721Abstract: Provided are a fluorescent on/off switchable compound for a gene analysis, which is highly stable and highly sensitive, and enables amplification and observation of a trace gene signal, and a labeling reagent for detection of a bio-related material, which uses the fluorescent on/off switchable compound. A compound represented by the following formula (I?): wherein X is a hydrogen atom or a carboxylic acid-protecting group, Y is a 2,4-dinitrobenzenesulfonyl group, a 2-cyano-4-nitrobenzenesulfonyl group or a 5-nitropyridin-2-ylsulfonyl group, R1, R2, R3 and R4 are each independently a hydrogen atom, a halogen atom, a hydroxyl group, an alkyl group having a carbon number of 1 to 6, an alkoxy group having a carbon number of 1 to 6, an aryl group having a carbon number of 6 to 10, or a cyano group.Type: GrantFiled: October 1, 2010Date of Patent: April 29, 2014Assignee: RikenInventors: Hiroshi Abe, Yoshihiro Ito, Aya Shibata, Mika Ito
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Publication number: 20140080127Abstract: The present invention provides heteroaryl functionalized cyanine dyes including a reactive functional moiety, or which are conjugated to a carrier molecule.Type: ApplicationFiled: November 20, 2013Publication date: March 20, 2014Applicant: Pacific Biosciences of California, Inc.Inventors: Stephen YUE, Gene Shen, Wei-Chuan Sun
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Publication number: 20140080122Abstract: Systems and methods related to optical nanosensors comprising photoluminescent nanostructures are generally described. Generally, the nanosensors comprise a photoluminescent nanostructure and a polymer that interacts with the photoluminescent nanostructure. In some cases, the interaction between the polymer and the nanostructure can be non-covalent (e.g., via van der Waals interactions). The nanosensors comprising a polymer and a photoluminescent nanostructure may be particularly useful in determining the presence and/or concentration of relatively small molecules, in some embodiments. In addition, in some instances the nanosensors may be capable of determining relatively low concentrations of analytes, in some cases determining as little as a single molecule. In some embodiments, the interaction between the analyte and the nanosensor (e.g.Type: ApplicationFiled: July 15, 2013Publication date: March 20, 2014Applicant: Massachusetts Institute of TechnologyInventors: Michael S. Strano, Jong-Ho Kim, Jinqing Zhang, Daniel A. Heller
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Publication number: 20140072515Abstract: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.Type: ApplicationFiled: February 28, 2013Publication date: March 13, 2014Inventors: Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa R. Dworecki, Frank G. Lehmann, Marie Christine Nlend
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Patent number: 8660753Abstract: The present invention relates to a control method of a wheel alignment apparatus using an MDPS, which determines whether or not to cancel center alignment control due to a trouble or error is preferentially determined prior to each control step and then performs control when wheels of a vehicle having an MDPS mounted therein are aligned, such that the trouble or error is preferentially considered in the control priority, thereby increasing driver's convenience and improving safety performance for protecting the driver.Type: GrantFiled: May 2, 2013Date of Patent: February 25, 2014Assignee: Hyundai Mobis Co., Ltd.Inventor: Jeong Ku Kim
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Publication number: 20140039178Abstract: The present invention is directed towards the synthesis of high purity deuterated sugars, deuterated phosphoramidites, deuterated nucleobases, deuterated nucleosides, deuterated oligonucleotides, and deuterated RNA's of defined sequences which can exhibit biochemically useful and biologically valuable properties, thus having potential for therapeutic uses.Type: ApplicationFiled: July 31, 2012Publication date: February 6, 2014Inventors: Suresh C. Srivastava, Amy Yasko
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Patent number: 8642561Abstract: A generic structure for the peptides of the present invention includes A-X-B-C, where C is a cargo moiety, the B portion includes basic amino acids, X is a cleavable linker sequence, and the A portion includes acidic amino acids. The intact structure is not significantly taken up by cells; however, upon extracellular cleavage of X, the B-C portion is taken up, delivering the cargo to targeted cells. Cargo may be, for example, a contrast agent for diagnostic imaging, a chemotherapeutic drug, or a radiation-sensitizer for therapy. Cleavage of X allows separation of A from B, unmasking the normal ability of the basic amino acids in B to drag cargo C into cells near the cleavage event. X is cleaved extracellularly, preferably under physiological conditions. D-amino acids are preferred for the A and B portions, to minimize immunogenicity and nonspecific cleavage by background peptidases or proteases.Type: GrantFiled: December 7, 2011Date of Patent: February 4, 2014Assignee: The Regents of the University of CaliforniaInventors: Tao Jiang, Roger Y. Tsien
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Patent number: 8637658Abstract: Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection.Type: GrantFiled: September 27, 2012Date of Patent: January 28, 2014Assignee: Applied Biosystems, LLCInventors: Gregory J. Ewing, Khairuzzaman Bashar Mullah, Ronald Graham
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Patent number: 8637650Abstract: The invention relates to novel classes of nucleotides that can be used as substrates for enzymes, e.g. for labeling nucleic acids.Type: GrantFiled: November 5, 2004Date of Patent: January 28, 2014Assignee: Genovoxx GmbHInventors: Dmitry Cherkasov, Christian Hennig
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Patent number: 8633307Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: GrantFiled: April 22, 2010Date of Patent: January 21, 2014Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
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Patent number: 8617814Abstract: Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.Type: GrantFiled: July 9, 2012Date of Patent: December 31, 2013Assignee: The Regents of the University of CaliforniaInventors: Guillermo C. Bazan, Brent S. Gaylord, Shu Wang
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Patent number: 8614061Abstract: The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY-632 and DY-520XL.Type: GrantFiled: December 1, 2009Date of Patent: December 24, 2013Assignee: Qiagen, GmbHInventors: Werner Brabetz, Cornelia Weber
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Patent number: 8604186Abstract: Novel energy transfer dyes which can be used with shorter wavelength light sources are provided. These dyes include a donor dye with an absorption maxima at a wavelength between about 250 to 450 nm and an acceptor dye which is capable of absorbing energy emitted from the donor dye. One of the energy transfer dyes has a donor dye which is a member of a class of dyes having a coumarin or pyrene ring structure and an acceptor dye which is capable of absorbing energy emitted from the donor dye, wherein the donor dye has an absorption maxima between about 250 and 450 nm and the acceptor dye has an emission maxima at a wavelength greater than about 500 nm.Type: GrantFiled: January 30, 2012Date of Patent: December 10, 2013Assignee: Applied Biosystems, LLCInventor: Linda G. Lee
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Patent number: 8597881Abstract: Provided herein is a method for replacing the allyl group in a compound of formula R—O-allyl, R2N(allyl), RNH(allyl), RN(allyl)2 or R—S-allyl with a hydrogen, using a transition metal and one or more phosphine ligands.Type: GrantFiled: October 25, 2011Date of Patent: December 3, 2013Assignee: Illumina Cambridge LimitedInventors: John Milton, Xiaolin Wu, Mark Smith, Joseph Brennan, Colin Barnes, Xiaohai Liu, Silke Ruediger
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Patent number: 8574833Abstract: Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.Type: GrantFiled: November 26, 2008Date of Patent: November 5, 2013Assignee: Great Basin ScientificInventors: Robert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Patent number: 8552173Abstract: The present invention relates to compositions useful as probes and in other applications and methods of their use. In some embodiments, nucleotides are prepared and functionalized with dyes. In some embodiments a first molecule is functionalized with an alkynyl group, a second molecule is functionalized with an azide group, and said first and second molecules are mixed under conditions to form a conjugate with a 1,2,3-triazol group. In further embodiments, a nucleotide is functionalized with an alkynyl group, a dye is functionalized with an azide group, and mixing the nucleotide and the dye forms a conjugate capable of emitting light.Type: GrantFiled: August 22, 2011Date of Patent: October 8, 2013Assignee: Third Wave Technologies, Inc.Inventors: Zbigniev Skrzypczynski, Sarah R. Wayland
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Patent number: 8546309Abstract: The present invention concerns monomeric or polymeric linker molecules useful in biological and chemical applications, their synthesis, and the synthesis and use of derivatives of the linkers conjugated to a variety of detectable labels and other substances. The linkers may be used, for example, in conjunction with fluorescent labels, nucleic acid or nucleic acid analog probes, and solid phase systems, and to enhance the solubility of the conjugated molecules.Type: GrantFiled: June 30, 2006Date of Patent: October 1, 2013Assignee: Dako Denmark A/SInventor: Jesper Lohse
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Patent number: 8536319Abstract: This application provides fluorescent probes, substrates, kits and methods for detecting the presence or absence of an enzyme, such as ricin, that catalyzes the release of adenine from a GAGA RNA tetraloop.Type: GrantFiled: June 28, 2007Date of Patent: September 17, 2013Assignee: Albert Einstein College of Medicine of Yeshiva UniversityInventors: Vern L. Schramm, Setu Roday
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Patent number: 8530156Abstract: Linkers and methods for determining a nucleotide sequence of a reference oligonucleotide are provided.Type: GrantFiled: July 31, 2009Date of Patent: September 10, 2013Assignee: President and Fellows of Harvard CollegeInventors: George M. Church, A. Michael Sismour
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Patent number: 8530195Abstract: Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.Type: GrantFiled: June 22, 2010Date of Patent: September 10, 2013Assignee: Biotium, Inc.Inventors: Fei Mao, Wai-Yee Leung, Xing Xin
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Patent number: 8530634Abstract: The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below. The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention.Type: GrantFiled: May 5, 2010Date of Patent: September 10, 2013Assignee: ChemGenes CorporationInventors: Andrei Laikhter, Suresh C. Srivastava, Naveen P. Srivastava
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Publication number: 20130230466Abstract: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.Type: ApplicationFiled: February 28, 2013Publication date: September 5, 2013Inventors: Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa R. Dworecki, Frank G. Lehmann, Marie Christine Nlend
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Patent number: 8524503Abstract: The present invention relates to dyes in general. The present invention provides a wide range of dyes and kits containing the same, which are applicable for labeling a variety of biomolecules such as nucleic acids, cells and microorganisms. The present invention also provides various methods of using the dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.Type: GrantFiled: March 6, 2012Date of Patent: September 3, 2013Assignee: Biotium, Inc.Inventors: Fei Mao, Hye Eun Hoover, Wai-Yee Leung
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Patent number: 8519115Abstract: The present invention relates to compositions and methods for detection and quantification of individual target molecules in biomolecular samples. In particular, the invention relates to improved, stable nanoreporter probes that are capable of binding to and identifying target molecules based on the probes' uniquely detectable signal. Methods for identifying target-specific sequences for inclusion in the probes are also provided, as are methods of making and using such probes. Polynucleotide sequences of certain nanoreporter components are also provided. The probes can be used in diagnostic, prognostic, quality control and screening applications.Type: GrantFiled: August 13, 2009Date of Patent: August 27, 2013Assignee: NanoString Technologies, Inc.Inventors: Philippa J. Webster, Craig E. Dahl
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Patent number: 8513399Abstract: The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.Type: GrantFiled: October 2, 2008Date of Patent: August 20, 2013Assignee: Bioneer CorporationInventors: Hyun Bae Kim, Seong Youl Kim, Jun Mo Gil, Hae Joon Park, Han Oh Park
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Patent number: 8501931Abstract: The present invention is directed to convertible nucleosides and polymer supported convertible nucleosides for use in SNAr displacement reactions. The convertible nucleosides can be used to synthesize numerous substituted purine and pyrimidine derivatives.Type: GrantFiled: October 12, 2007Date of Patent: August 6, 2013Assignee: Research Foundation of the City University of New YorkInventors: Mahesh K. Lakshman, Suyeal Bae
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Publication number: 20130197209Abstract: The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3?-OH blocking group covalently attached thereto, such that the 3? carbon atom has attached a group of the structure —O—Z wherein Z is any of —C(R?)2-O—R?, —C(R?)2-N(R?)2, —C(R?)2-N(H)R?, —C(R?)2-S—R? and —C(R?)2-F, wherein each R? is or is part of a removable protecting group; each R? is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R?)2 represents an alkylidene group of formula ?C(R??)2 wherein each R?? may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R? is exchanged for H or, where Z is —C(R?) 2-F, the F is eType: ApplicationFiled: March 8, 2013Publication date: August 1, 2013Applicant: ILLUMINA CAMBRIDGE LIMITEDInventors: John MILTON, Xiaolin Wu, Mark Smith, Joseph Brennan, Colin Bames, Xiaohai Liu, Silke Ruediger
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Publication number: 20130184160Abstract: The present disclosure encompasses oligonucleotide aptamers selectively binding a target glycosylated polypeptide or protein, and having biased affinity for the glycan through a boronic acid linked to a nucleosidic base of a nucleotide(s). The disclosure further encompasses methods for isolating an aptamer(s) selectively binding a target glycosylated polypeptide, where, from a population of randomized oligonucleotides that have at least one nucleotide having a boronic acid label linked to a base, is selected a first subpopulation of aptamers binding to the target glycosylated polypeptide or protein. This subpopulation is then amplified without using boronic acid-modified TTP, and amplification products not binding to a target glycosylated polypeptide or protein are selected. The second subpopulation of aptamers is then amplified using boronic acid-modified TTP to provide a population of boronic acid-modified aptamers capable of selectively binding to a glycosylation site of a target polypeptide or protein.Type: ApplicationFiled: July 17, 2008Publication date: July 18, 2013Inventors: Binghe Wang, Minyong Li, Zhen Huang, Na Lin
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Patent number: 8486620Abstract: Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.Type: GrantFiled: April 29, 2011Date of Patent: July 16, 2013Assignee: Ventana Medical Systems, Inc.Inventors: Jerry W. Kosmeder, Casey A. Kernag, Donald Johnson, Christopher Bieniarz
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Patent number: 8445191Abstract: Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.Type: GrantFiled: May 2, 2011Date of Patent: May 21, 2013Assignee: Ventana Medical Systems, Inc.Inventors: Jerome W. Kosmeder, Casey A. Kernag, Donald Johnson, Christopher Bieniarz
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Patent number: 8440399Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: GrantFiled: August 25, 2009Date of Patent: May 14, 2013Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
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Patent number: 8435735Abstract: The present invention relates to methods and compounds for detection of molecular targets, such as biological or chemical molecules, or molecular structures, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELISA, Southern, Northern, and Western blotting, etc.Type: GrantFiled: February 19, 2010Date of Patent: May 7, 2013Assignee: Dako Denmark A/SInventor: Jesper Lohse
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Patent number: 8426569Abstract: The present invention relates to unnatural base pairs of Ds (a 7-(2 thienyl)-3H-imidazo[4,5-b]pyridine-3-yl group) and a Pa derivative (a 2-nitro-1H-pyrrole-1-yl group bearing a substituent having a ?-electron system attached at position 4) that can be replicated with high selectivity/high efficiency, and methods for replicating nucleic acids containing the unnatural base pairs. The present invention also relates to methods for incorporating an unnatural base bearing a functional substituent attached thereto into DNA by a nucleic acid replication reaction. The present invention also relates to methods for replicating and selectively collecting a nucleic acid containing an unnatural base pair from a nucleic acid pool. The present invention also relates to methods for determining a sequence of natural bases in the proximity of an unnatural base in DNA for achieving highly efficient and highly selective replication of a nucleic acid containing the unnatural base.Type: GrantFiled: March 31, 2009Date of Patent: April 23, 2013Assignees: Riken, Tagcyx BiotechnologiesInventors: Ichiro Hirao, Michiko Hirao, Shigeyuki Yokoyama
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Patent number: 8399188Abstract: The invention provides nucleoside and nucleotide molecules containing cleavable linkers linking a label such as a dye. The invention also provides nucleosides and nucleotide molecules containing a blocking group, either removable or non-removable. The invention additionally provides methods of using the nucleoside and nucleotide molecules containing a cleavable linker and/or a blocking group.Type: GrantFiled: September 4, 2007Date of Patent: March 19, 2013Assignee: Illumina, Inc.Inventors: Chanfeng Zhao, Igor Kozlov, Melissa D. Shults
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Patent number: 8394949Abstract: The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties.Type: GrantFiled: March 6, 2009Date of Patent: March 12, 2013Assignee: Enzo Life Sciences, Inc.Inventors: Elazar Rabbani, Jannis G. Stavrianopoulos, James J. Donegan
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Patent number: 8389745Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.Type: GrantFiled: August 29, 2011Date of Patent: March 5, 2013Assignee: Elitech Holding B.V.Inventors: Eugeny Lukhtanov, Alexei Vorobiev
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Patent number: 8389702Abstract: The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties.Type: GrantFiled: June 24, 2010Date of Patent: March 5, 2013Assignee: Enzo Life Sciences, Inc.Inventors: Elazar Rabbani, Jannis G. Stavrianopoulos, James J. Donegan
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Multisignaling oligomeric or polymeric compositions comprising labeled moieties and binding partners
Patent number: 8389701Abstract: The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties.Type: GrantFiled: June 24, 2010Date of Patent: March 5, 2013Assignee: Enzo Life Sciences, Inc.Inventors: Elazar Rabbani, Jannis G. Stavrianopoulos, James J. Donegan -
Patent number: 8383792Abstract: The present invention provides, for example, a labeling substance that allows the double helix structure of a nucleic acid to be detected effectively. The present invention provides a compound having a structure derived from mononucleoside or mononucleotide, with the structure being represented by the following formula (1), (1b), or (1c), a tautomer or stereoisomer thereof, or a salt thereof. In the above formulae, B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 each are a hydrogen atom, a protecting group, or an atomic group that exhibits fluorescence and may be identical to or different from each other.Type: GrantFiled: March 6, 2008Date of Patent: February 26, 2013Assignee: RikenInventors: Akimitsu Okamoto, Shuji Ikeda, Takeshi Kubota
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Patent number: 8383795Abstract: The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5? to 3? nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.Type: GrantFiled: March 3, 2011Date of Patent: February 26, 2013Assignee: Sequenom, Inc.Inventors: Dirk Johannes Van Den Boom, Paul Andrew Oeth, Payam Mahboubi
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Patent number: 8367813Abstract: The present invention provides labeled phospholink nucleotides that can be used in place of naturally occurring nucleotide triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions and various analyzes based thereon, including DNA sequencing, single base identification, hybridization assays, and others.Type: GrantFiled: November 17, 2009Date of Patent: February 5, 2013Assignee: Pacific Biosciences of California, Inc.Inventor: Jonas Korlach
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Patent number: 8357793Abstract: This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents can be usefully applied to protein and nucleic acid probe based assays. They are also applicable to real-time detection processes.Type: GrantFiled: January 22, 2004Date of Patent: January 22, 2013Assignee: Enzo Life Sciences, Inc.Inventors: Jannis G. Stavrianopoulos, Flazar Rabbani
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Patent number: 8354515Abstract: A labeling agent comprising a plurality of nucleotide derivative units represented by Formula (1) below: (where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group, X1 represents O, S or Se, and X2 represents SH (or S?), Se?, a C1-4 alkyl or a morpholino group).Type: GrantFiled: November 2, 2009Date of Patent: January 15, 2013Assignee: Japan Science and Technology AgencyInventors: Yoshihito Ueno, Yukio Kitade
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Patent number: 8354252Abstract: Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions, that include structural components that maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated.Type: GrantFiled: May 25, 2011Date of Patent: January 15, 2013Assignee: Pacific Biosciences of California, Inc.Inventors: Jeffrey Wegener, Jonas Korlach
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Patent number: 8354523Abstract: The present invention provides a dually labeled oligonucleotide probe, methods of preparing and using the same. The subject probes are particularly useful for high-sensitive nucleic acid detection via hybridization assays including but not limited to template-directed polymerization reactions.Type: GrantFiled: July 19, 2007Date of Patent: January 15, 2013Assignee: Allelogic Biosciences CorporationInventors: Fei Mao, Xing Xin
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Patent number: 8349556Abstract: Aspects of the invention provide novel and surprisingly effective methods for the detection of nucleic acids, comprising nucleic acid amplification using base-modified deoxynucleoside 5?-triphosphates (dNTPs). Particular aspects relate to methods for enhancing hybridization properties of oligonucleotide primers and probes in assays detecting nucleic acids, comprise amplifying target DNAs in presence of base-modified duplex-stabilizing deoxyribonucleoside 5?-triphosphates to provide for modified target DNAs, and thereby considerably improving performance of the detection assays. The disclosed methods allow for increasing of the reaction temperature in PCR-based detection systems or, alternatively, reducing the length of the oligonucleotide primers and probes. Certain aspects relates to improvement of real time PCR assays, wherein nucleic acids of interest are detected as the reaction proceeds using fluorescent agents or oligonucleotide FRET probes.Type: GrantFiled: April 30, 2007Date of Patent: January 8, 2013Inventor: Igor Kutyavin
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Patent number: 8344118Abstract: The synthesis of capped/tagged RNA, methods of use and kits providing same are contemplated. Tagged RNA permits isolation of RNA transcripts in vitro. The ability to isolate and purify capped RNA results in improved transcription and translation and provides a tool for identifying RNA-protein interactions. Such capped RNA finds use in therapeutic applications, diagnosis and prognosis and in the treatment of cancers and HIV.Type: GrantFiled: December 13, 2011Date of Patent: January 1, 2013Assignee: Applied Biosystems, LLCInventors: Anilkumar Kore, Shanmugasundaram Muthian, Irudaya Charles