Bird (e.g., Chicken, Etc.) Patents (Class 800/19)
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Patent number: 8431770Abstract: Transgenic avians which produce proteins in their oviduct tissue having modified oligosaccharide structures and methods of making such avians are disclosed herein. The invention also includes the modified proteins produced in the transgenic birds.Type: GrantFiled: January 7, 2009Date of Patent: April 30, 2013Assignee: Synageva BioPharma Corp.Inventor: Alex J. Harvey
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Publication number: 20130023653Abstract: Transgenes encoding exogenous antibodies are stably integrated into donor cells and are present in the somatic tissue of chimeric birds. The transgenes encode exogenous antibodies and are preferably expressed in the oviduct for collection in the egg. Tissue specificity is provided by selecting the content of the transgene accordingly. Birds whose genome is comprised of trangene-derived exogenous antibody-encoding DNA express exogenous antibodies having desirable chemical properties with increased therapeutic utility compared to antibodies derived from bacterial expression systems.Type: ApplicationFiled: July 16, 2012Publication date: January 24, 2013Inventors: Lei Zhu, Wen Zhou, Robert J. Etches
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Publication number: 20120331576Abstract: The use of interferon induced transmembrane protein 1, 2, or 3 (IFITM1, 2, or 3) as a viral restriction factor, and methods of using the same to produce virus, transgenic animals expressing exogenous IFITM1, 2, or 3, and methods of treating or inhibiting viral infections by targeting a gene identified hereinType: ApplicationFiled: December 10, 2010Publication date: December 27, 2012Applicants: THE GENERAL HOSPITAL CORPORATION, THE BRIGHAM AND WOMEN'S HOSPITAL, INC.Inventors: Abraham Brass, Stephen Elledge
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Patent number: 8324447Abstract: The present invention relates to animal model systems comprising a chimera between an avian embryo and a mammalian organism. Specifically, chimeric model systems comprising normal, diseased or genetically transformed mammalian cells and tissues transplanted into avian embryos, and uses thereof for in vivo testing of drugs and therapeutic modalities are disclosed.Type: GrantFiled: June 28, 2005Date of Patent: December 4, 2012Assignees: Bar Ilan University, Medical Research Fund of Tel-Aviv Sourasky Medical CenterInventors: Ron Goldstein, Tamar Tennenbaum, Varda Deutsch
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Publication number: 20120266265Abstract: This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of avians in order to express the exogenous sequences to alter the phenotype of the avians or to produce desired proteins. In particular, transgenic avians are produced which express exogenous sequences in their oviducts and which deposit exogenous proteins into their eggs. Avian eggs that contain exogenous proteins are encompassed by this invention. The instant invention further provides novel forms of interferon and erythropoietin which are efficiently expressed in the oviduct of transgenic avians and deposited into avian eggs.Type: ApplicationFiled: June 24, 2011Publication date: October 18, 2012Inventors: Robert D. Ivarie, Alex J. Harvey, Julie A. Morris, Guodong Liu, Jeffrey C. Rapp
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Patent number: 8283518Abstract: Methods and compositions for the administration of transposon-based vectors to the reproductive organs of animals and the creation of transgenic animals. Preferred methods involve administration of the transposon-based vectors to the lumen of the oviduct of an avian, expression of a vector derived transgene in the avian, and deposition of the resultant polypeptide in an egg. This invention allows for large amounts of protein to be deposited in the egg.Type: GrantFiled: October 31, 2007Date of Patent: October 9, 2012Assignees: TransGenRx, Inc., The Board of Supervisors of Louisiana State University and Agricultural and Mechanical CollegeInventors: Richard K. Cooper, William C. Fioretti, Gary G. Cadd
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Publication number: 20120222143Abstract: Compositions and methods for use of TALENs to make genetically modified livestock are set forth. The methods may include reporters for selecting cells or embryos that have been modified by TALENs for use as progenitor cells to make founder animals.Type: ApplicationFiled: February 24, 2012Publication date: August 30, 2012Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Publication number: 20120185954Abstract: This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.Type: ApplicationFiled: November 4, 2011Publication date: July 19, 2012Applicant: INTREXON CorporationInventors: Subba Reddy PALLI, Mohan Basavaraju Kumar
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Patent number: 8222032Abstract: The methods of the present invention involve the manipulation and/or propagation of oviduct tumor cells derived from either wild-type or transgenic avians.Type: GrantFiled: June 5, 2009Date of Patent: July 17, 2012Assignee: Synageva BioPharma Corp.Inventors: Stephen H. Parker, Yashwant M. Deo
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Patent number: 8198082Abstract: A chicken embryonic stem cell is established, which stably has pluripotency and an ability of being differentiated into a germ cell. For evaluating on whether or not the chicken embryonic stem cell can be applied to genetic modification technique, detection is made on a protein which serves as an indicator of the ability of being differentiated into a germ cell. This provides (i) a chicken embryonic stem cell applicable to genetic modification technique and (ii) a method for evaluation of the chicken embryonic stem cell.Type: GrantFiled: March 26, 2008Date of Patent: June 12, 2012Assignees: Hiroshima University, Hiroshima Industrial Promotion OrganizationInventors: Hiroyuki Horiuchi, Haruo Matsuda, Shuichi Furusawa, Mikiharu Nakano, Yusuke Yamashita, Masaki Nishimoto
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Publication number: 20120084873Abstract: The present invention relates generally to the field of sex determination of animals. Provided are methods and agents to manipulate sex determination, particularly in avian animals such as chickens, through a male chromosome-linked testis (sex) regulatory gene. Expression or activity of the DMRT1 gene or protein is modulated to produce animals with displaying a phenotype sex that differs from their genotype.Type: ApplicationFiled: February 8, 2010Publication date: April 5, 2012Inventors: Andrew Sinclair, Craig Smith, Timothy James Doran, Robert John Moore, John William Lowenthal
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Publication number: 20120073005Abstract: The present invention relates to a transgenic animal which intracellularly expresses at least two types of foreign functional RNA and has resistance to a pathogen such as a virus. In the animal carrying multiple types of foreign functional RNA, the proliferation of a pathogen can be more significantly inhibited compared to an animal carrying one type of foreign functional RNA.Type: ApplicationFiled: April 28, 2010Publication date: March 22, 2012Applicant: KANEKA CORPORATIONInventors: Hiroyuki Watanabe, Maki Masutani, Kenji Kyogoku
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Publication number: 20110296541Abstract: Methods for preparing recombinant proteins, such as antibodies, in eggs are described. The method offers advantages over existing systems for preparing recombinant proteins including high yield, low cost and compatibility with animal protection regulations. In addition, since eggs are edible food sources the recombinant protein does not have to be isolated from the egg.Type: ApplicationFiled: February 4, 2011Publication date: December 1, 2011Inventors: Robert J. Etches, Mansoor Mohammed, Sherie Morrison, Letitia A. Wims, Kham M. Trinh, Alan G. Wildeman
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Publication number: 20110277048Abstract: Human antibodies are produced in transgenic chickens having functional genetic components of the human immune system stably integrated in the genome. Techniques are described to integrate unrearranged human heavy and light chain immunoglobulin loci into the genome of the transgenic chicken. The functional description of the endogenous immunoglobulin loci yields an animal whose antibody repertoire in response to antigen is fully human. In the preferred embodiment, the human immunoglobulin locus is at least as large as the native human locus and exhibits functional class switching to yield IgG isotype antibodies. In addition to monoclonal antibodies secreted from immortalized B cell populations, polyclonal antibodies may be obtained from eggs laid by the transgenic chickens of the invention.Type: ApplicationFiled: July 13, 2010Publication date: November 10, 2011Inventors: Robert J. Etches, Robert M. Kay, Philip Leighton, Lei Zhu
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Publication number: 20110247091Abstract: The present disclosure provides a transgenic cell or chicken having increased resistance to RNA viral infection, wherein the chicken cell expresses a foreign RIG-I gene. Also provided are methods for reducing RNA virus replication in a chicken cell and methods for producing the transgenic cell or chicken with increased RNA viral resistance.Type: ApplicationFiled: March 25, 2011Publication date: October 6, 2011Inventors: Katharine Edens Magor, Megan Rani Winnifred Barber, Jerry Ronald Aldridge, JR., Robert G. Webster
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Publication number: 20110239317Abstract: The present invention provides isolated polynucleotides encoding promyostatin polypeptides or a peptide portion thereof, polynucleotides complementary thereto, and oligonucleotides that can specifically hybridize to such polynucleotides. The present invention also provides an isolated polynucleotide encoding a mature myostatin peptide.Type: ApplicationFiled: January 26, 2011Publication date: September 29, 2011Inventors: Se-Jin Lee, Alexandra C. McPherron
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Patent number: 7994388Abstract: Methods for modulating primordial germ cell (PGC) numbers and/or development in avians are provided. In one embodiment, the presently disclosed subject matter provides a method for modulating primordial germ cells numbers in an avian embryo comprising immunizing a female bird with an antigen associated with primordial germ cells, whereby an egg produced by the female bird comprises a sufficiently high concentration of antibodies specific for the antigen to modulate numbers of endogenous PGCs in an avian embryo present within in the egg. Also provided are methods for producing chimeric avians, methods for increasing the proportion of male birds in a plurality of eggs, methods of producing avian gametes, and methods for enhancing germ line transmission of nucleic acids in birds.Type: GrantFiled: January 16, 2004Date of Patent: August 9, 2011Assignee: North Carolina State UniversityInventors: James N. Petitte, Samuel Lloyd Pardue
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Publication number: 20110162096Abstract: Methods and compositions are presented for the administration of transposon-based vectors to an animal or human to provide gene therapy to the animal or humanType: ApplicationFiled: November 8, 2010Publication date: June 30, 2011Applicants: College, TransGenRx, Inc.Inventors: Richard Cooper, Frederick M. Enright, William C. Fioretti
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Publication number: 20110119782Abstract: The present invention relates generally to constructs and in particular genetic constructs comprising polynucleotide sequences capable of release in covalently closed, circular form from a larger nucleotide sequence such as, but not limited to, a genome of a eukaryotic cell. Preferably, once released, a polynucleotide sequence is reconstituted in a form which permits expression of the polynucleotide sequence. In one embodiment, the reconstituted polynucleotide sequence comprises a coding sequence with all or part of an extraneous nucleotide such as, but not limited to, an intronic sequence or other splice signal inserted therein. Expression and in particular transcription of the coding sequence involves splicing out the extraneous sequence. The release and circularization is generally in response to a stimulus such as a protein-mediated stimulus. More particularly, the protein is a viral or prokaryotic or eukaryotic derived protein or developmentally and/or tissue specific regulated protein.Type: ApplicationFiled: November 17, 2010Publication date: May 19, 2011Applicant: Queensland University of TechnologyInventors: James Langham Dale, Benjamin Dugdale, Greg John Hafner, Scott Richard Hermann, Douglas Kenneth Becker, Robert Maxwell Harding, Srimek Chowpongpang
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Publication number: 20110088106Abstract: Hypercellular nonhuman organisms have functionally inactivated expression of a cyclin inhibitor gene, especially p27. The growth rate of nonhuman organisms are increased such that a desired size is attained more quickly than as compared to nonvariant organisms. Inhibitors of the p27 cyclin dependent kinase inhibitor protein or sequences encoding the protein modulate vertebrate cell cycle progression and increase the proportion of dividing cells to non-dividing cells in a population of treated cells. As the proportion of dividing cells increases, the cell population, e.g., hematopoietic progenitor (stem) cells, is more efficiently used for gene therapy applications. Transgenic animals and plants, and knockout alleles are provided.Type: ApplicationFiled: July 14, 2009Publication date: April 14, 2011Applicant: Fred Hutchinson Cancer Research CenterInventors: James M. Roberts, Steven R. Coats, Matthew L. Fero
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Publication number: 20110023160Abstract: A transgenic chicken is disclosed having disrupted endogenous immunoglobulin production. In one embodiment, a targeting construct is stably integrated into the genome of the chicken by homologous recombination in embryonic stem cells, and injection of the engineered embryonic stem cells into recipient embryos, thereby knocking out the endogenous immunoglobulin gene locus in resulting animals. The targeted disruption of the locus in embryonic stem cells is particularly useful in combination with the insertion of genetic elements encoding exogenous immunoglobulin molecules. After these chickens are cross-bred, a line of chickens is produced that has a reduction of endogenous immunoglobulin molecule production.Type: ApplicationFiled: October 1, 2010Publication date: January 27, 2011Inventors: Robert J. Etches, Robert M. Kay, Lei Zhu
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Patent number: 7875762Abstract: This invention provides for proteins which are expressed in the avian oviduct, packaged into eggs laid by the avian and removed from the eggs.Type: GrantFiled: January 23, 2006Date of Patent: January 25, 2011Assignees: Synageva BioPharma Corp., University of Georgia Research Foundation, Inc.Inventors: Robert D. Ivarie, Alex J. Harvey, Julie A. Morris, Guodong Liu, Jeffrey C. Rapp
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Publication number: 20110016545Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: August 1, 2008Publication date: January 20, 2011Applicant: VERENIUM CORPORATIONInventors: Kevin Gray, Reinhard Dirmeier
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Publication number: 20100303806Abstract: The invention encompasses among other things antibodies including cytotoxic antibodies such as anti-CD20 having avian N-linked glycosylation patterns obtained from egg white of eggs laid by transgenic avians.Type: ApplicationFiled: May 27, 2010Publication date: December 2, 2010Inventor: Alex J. Harvey
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Publication number: 20100306869Abstract: The present invention relates to methods of modulating traits, particularly production traits, in avians such as chickens. In particular, the invention relates to the in ovo delivery of a dsRNA molecule, especially siRNAs, to modify production traits in commercially important birds.Type: ApplicationFiled: June 12, 2008Publication date: December 2, 2010Applicants: COMMWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANIZATION, AUSTRALIAN POULTRY CRC PTY LTDInventors: Timothy James Doran, Robert John Moore, John William Lowenthal
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Patent number: 7812215Abstract: Methods of producing protein using a recombinant ovomucoid gene expression controlling region operably linked to one or more useful amino acid coding sequences.Type: GrantFiled: November 17, 2008Date of Patent: October 12, 2010Assignee: Synageva BioPharma Corp.Inventors: Alex J. Harvey, Markley C. Leavitt, Youliang Wang
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Publication number: 20100235929Abstract: The invention relates to compositions and methods for producing antibodies having human idiotypes in non-human animals.Type: ApplicationFiled: May 30, 2008Publication date: September 16, 2010Applicant: Buelow Research Enterprises LLCInventor: Roland Buelow
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Patent number: 7795496Abstract: The present invention provides for methods of producing transgenic avians which may include delivering a heterologous nucleic acid to oviduct tissue of an avian wherein the nucleic acid enters a cell of the oviduct tissue and is expressed.Type: GrantFiled: February 13, 2009Date of Patent: September 14, 2010Assignee: Synageva BioPharma Corp.Inventors: Leandro Christmann, Markley C. Leavitt, Dawn M. Eberhardt, Timalynn M. Matthews
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Publication number: 20100205682Abstract: The present invention concerns methods and means to produce humanized antibodies from transgenic non-human animals. The invention specifically relates to novel immunoglobulin heavy and light chain constructs, recombination and transgenic vectors useful in making transgenic non-human animals expressing humanized antibodies, transgenic animals, and humanized immunoglobulin preparations.Type: ApplicationFiled: July 29, 2009Publication date: August 12, 2010Inventors: ROLAND BUELOW, Wim van Schooten, Josef Platzer
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Publication number: 20100199366Abstract: Methods and compositions are presented for the administration of transposon based vectors to an animal or human to provide gene therapy to the animal or human.Type: ApplicationFiled: December 24, 2004Publication date: August 5, 2010Inventors: Richard Cooper, Frederick M. Enright, William C. Fioretti
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Publication number: 20100192241Abstract: Methods for preparing recombinant proteins, such as antibodies, in eggs are described. The method offers advantages over existing systems for preparing recombinant proteins including high yield, low cost and compatibility with animal protection regulations. In addition, since eggs are edible food sources the recombinant protein does not have to be isolated from the egg.Type: ApplicationFiled: July 27, 2009Publication date: July 29, 2010Inventors: Robert J. Etches, Mansoor Mohammed, Sherie Morrison, Letitia A. Wims, Kham M. Trinh, Alan G. Wildeman
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Patent number: 7754479Abstract: The present invention relates to a method for long-term culturing of avian spermatogonial stem cells, which comprises the steps of: (a) preparing an avian testis; (b) isolating a population of testicular cells from said avian testis; and (c) culturing said avian spermatogonial stem cells in said population of testicular cells on a feeder cell layer in a medium containing a cell growth factor, a population of avian spermatogonial stem cells and a method for producing transgenic ayes.Type: GrantFiled: August 6, 2004Date of Patent: July 13, 2010Assignees: Seoul National University Industry Foundation, Avicore Biotechnology Institute Inc.Inventors: Jae Yong Han, Yeong Ho Hong, Jeong Mook Lim, Young Mok Lee, Mak Soon Lee, Jin Gyung Jung
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Publication number: 20100138946Abstract: The present invention is transgenic chickens obtained from long-term cultures of avian PGCs and techniques to produce and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs are transmitted through the germline to yield transgenic offspring. This invention includes compositions comprising long-term cultures of PGCs and offspring derived from them that are genetically modified.Type: ApplicationFiled: August 14, 2008Publication date: June 3, 2010Inventors: Marie-Cecile Van de Lavoir, Philip Albert Leighton
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Publication number: 20100083389Abstract: The invention includes methods of making transgenic avians by introducing a nucleic acid sequence into the genome of an avian embryo wherein the nucleic acid sequence comprises a nucleotide sequence encoding a heterologous protein, developing the avian embryo to hatch, developing the hatched chick to sexual maturity and obtaining offspring from the sexually mature hatched chick which produce egg white containing the heterologous protein.Type: ApplicationFiled: May 1, 2009Publication date: April 1, 2010Inventors: Jeffrey C. Rapp, Leandro Christmann, Alex J. Harvey, Markley C. Leavitt
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Publication number: 20100077491Abstract: Provided herein are compositions and methods relating to the involvement of RNF5 in muscle wasting.Type: ApplicationFiled: July 12, 2007Publication date: March 25, 2010Applicants: Burnham Institute for Medical Research, Mount Sinai School of MedicineInventors: Ze'ev Ronai, Agnes DeLaunay, Kenneth Bromberg
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Publication number: 20090313712Abstract: The invention relates to transgenic avians whose genome contains nucleotide sequences which encode therapeutic polynucleotides that correspond to one or more certain sequences in the genome of an avian pathogen.Type: ApplicationFiled: April 1, 2009Publication date: December 17, 2009Inventors: Leandro Christmann, Rapp C. Jeffrey
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Publication number: 20090305384Abstract: The invention provides histone deacetylase class II nucleic acids and polypeptides, methods and reagents for their use, and related compounds including small molecule libraries containing class II histone deacetylase inhibitors.Type: ApplicationFiled: February 12, 2009Publication date: December 10, 2009Applicant: President and Fellows of Harvard CollegeInventors: Christina M. Grozinger, Christian A. Hassig, Stuart L. Schreiber
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Publication number: 20090307790Abstract: The present invention relates to a method for producing a transgenic non human male animal, preferably a mammal, fish, bird or insect, wherein the transgene(s) confer(s) a change in the transmission ratio of (a) genetic trait(s) to the offspring of said non human male animal, preferably mammal, fish, bird or insect to a non-Mendelian ratio, said method comprising introducing (a) a first nucleic acid molecule encoding an expression product with a Responder function into a chromosome of a non-human germ cell, (fertilized) egg cell, embryonic cell or a cell derived therefrom, of the same species as the transgenic male to be prepared, said chromosome containing or conferring said genetic trait(s), thereby linking on said chromosome said Responder function to the genetic trait(s); and (b) at least one second nucleic acid molecule encoding an expression product with a Distorter function into (a) chromosome(s) of a non-human germ cell, (fertilized) egg cell, embryonic cell or a cell derived therefrom, of the same spType: ApplicationFiled: August 12, 2006Publication date: December 10, 2009Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V.Inventors: Bernhard Herrmann, Hermann Bauer
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Patent number: 7608451Abstract: Administration of modified transposon-based vectors has been used to achieve stable incorporation of exogenous genes into animals. These transgenic animals produce transgenic progeny. Further, these transgenic animals produce large quantities of desired molecules encoded by the transgene. Transgenic egg-laying animals produce large quantities of desired molecules encoded by the transgene and deposit these molecules in the egg.Type: GrantFiled: October 31, 2007Date of Patent: October 27, 2009Assignees: Transgen Rx, Inc., The Board of Supervisors of Louisiana State University and Mechanical CollegeInventors: Richard K. Cooper, Gary G. Cadd, William C. Fioretti, Kenneth F. De Boer
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Publication number: 20090210953Abstract: The present invention relates to the elucidation that TRPML3 is involved in salty taste perception in primates including humans and likely other mammals and based thereon high-throughput mammalian and medium-throughput oocyte-based electrophysiological assays for identifying human TRPML3 modulators, preferably TRPML3 enhancers. Compounds that modulate TRPML3 function in the assay are expected to affect salty taste in humans. The inventive electrophysiological assays, such as the two-electrode voltage-clamp technique, facilitate the identification of compounds which specifically modulate human TRPML3. The assays of the invention provide a robust screen useful to detect compounds that facilitate (enhance) or inhibit TRPML3 function. Compounds that enhance or block TRPML3 channel activity should thereby modulate salty taste. In addition, these compounds may be used to regulate sodium excretion, urinary output and other biological functions relating to sodium levels and TRPML3 related functions.Type: ApplicationFiled: December 5, 2008Publication date: August 20, 2009Inventors: Bryan Moyer, Albert Zlotnik, Peter Hevezi, Hortensia Soto, Dalia Kalabat, Min Lu, Na Gao, Guy Servant, Evan Carl White, Paul Brust, Mark Williams
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Publication number: 20090188002Abstract: The invention includes avians containing an artificial chromosome in their genome and methods of making the avians.Type: ApplicationFiled: December 12, 2008Publication date: July 23, 2009Inventors: Leandro Christmann, Dawn M. Eberhardt, Markley C. Leavitt, Alex J. Harvey
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Publication number: 20090178147Abstract: Transgenic avians which produce proteins in their oviduct tissue having modified oligosaccharide structures and methods of making such avians are disclosed herein. The invention also includes the modified proteins produced in the transgenic birds.Type: ApplicationFiled: January 7, 2009Publication date: July 9, 2009Inventor: Alex J. Harvey
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Publication number: 20090165153Abstract: Methods and means for efficiently downregulating the expression of any gene of interest in eukaryotic cells and organisms are provided. To this end, the invention provides modified antisense and sense RNA molecules, chimeric genes encoding such modified antisense or sense RNA molecules and eukaryotic organisms such as plants, animals or fungi, yeast or molds, comprising the modified antisense and/or sense RNA molecules or the encoding chimeric genes.Type: ApplicationFiled: July 18, 2008Publication date: June 25, 2009Applicant: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANIZATION (CSIRO)Inventors: Ming-Bo Wang, Peter Waterhouse
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Publication number: 20090165155Abstract: Transgenes encoding exogenous proteins are stably integrated into embryonic stem cells and are present in the somatic tissue of transgenic or chimeric birds. The transgenes encode exogenous proteins and are expressed in any of endodermal, ectodermal, mesodermal, or extra embryonic tissue. Tissue specificity is provided by selecting the content of the transgene accordingly. Transgenic birds whose genome is comprised of trangene derived exogenous DNA express exogenous proteins with tissue specificity, and specifically express exogenous proteins in the tubular gland cells of the oviduct to concentrate exogenous proteins in egg white.Type: ApplicationFiled: April 17, 2008Publication date: June 25, 2009Inventors: Lei Zhu, Peggy Winters-Digiacinto, Robert J. Etches
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Patent number: 7550650Abstract: This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.Type: GrantFiled: October 2, 2003Date of Patent: June 23, 2009Assignee: Synageva BioPharma Corp.Inventors: Jeffrey C. Rapp, Leandro Christmann
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Publication number: 20090155238Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: February 14, 2007Publication date: June 18, 2009Applicant: Verenium CorporationInventors: David Weiner, David Blum, Alexander Varvak, Shaun Healey, Kristine Chang, Geoff Hazlewood, Thomas Todaro, Grace Desantis, Hwai Chang, Connie Jo Hansen, Scott W. Beaver, Thomas Woodward, Charles Hancock
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Publication number: 20090158449Abstract: The present invention has for its object to provide a transgenic bird with a foreign gene containing a feline-derived protein-encoding sequence as transferred therein, and a method of producing the same. The present invention provides a method of producing a feline-derived protein by using a transgenic bird with a method which comprises infecting an avian embryo with a replication defective retrovirus vector containing a foreign gene by microinjection thereof into the early heart or blood vessel formed in the embryo and allowing the embryo to hatch.Type: ApplicationFiled: August 30, 2006Publication date: June 18, 2009Applicant: KANEKA CORPORATIONInventors: Tomoyuki Nakaishi, Takuya Shindo, Tomoko Awa
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Patent number: 7541512Abstract: The invention provides for transgenic avians containing nucleic acids which include an exogenous lysozyme gene expression controlling nucleotide sequence which typically is linked to a polynucleotide encoding a heterologous polypeptide.Type: GrantFiled: January 26, 2007Date of Patent: June 2, 2009Assignee: Synageva BioPharma Corp.Inventors: Jeffrey C. Rapp, Alex J. Harvey
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Publication number: 20090133134Abstract: The present invention is directed to a vector and its use to generate genetically modified animals and cells. One aspect of this invention involves a vector that comprises a sperm cell and one or more polynucleotide molecules bound to a sperm cell through one or more sperm antibody linker. The sperm cell can be any animal sperm cell, preferably non-human animal such as a mouse, pig, sheep, goat, or chicken. In one preferred embodiment of this invention, the one or more polynucleotide molecules encode for a gene product that confers desired characteristics in the cells or the animals. In another preferred embodiment of this invention, the genetically modified cells are able to produce desired therapeutic proteins. The association of the sperm, linker, and the one or more polynucleotide can also occur in vitro or in vivo. In another embodiment, the genetically modified cells are transgenic chicken eggs in which one or more desired recombinant protein is expressed.Type: ApplicationFiled: February 22, 2008Publication date: May 21, 2009Inventor: Kangsheng Wang
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Patent number: 7534929Abstract: This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of avians in order to express the exogenous sequences to alter the phenotype of the avians or to produce desired proteins. In particular, transgenic avians are produced which express exogenous sequences in their oviducts and which deposit exogenous proteins into their eggs. Avian eggs that contain exogenous proteins are encompassed by this invention. The instant invention further provides novel forms of interferon and erythropoietin which are efficiently expressed in the oviduct of transgenic avians and deposited into avian eggs.Type: GrantFiled: April 6, 2005Date of Patent: May 19, 2009Assignees: Synageva BioPharma Corp., University of Georgia Research Foundation, Inc.Inventors: Robert D. Ivarie, Alex J. Harvey, Julie A. Morris, Guodong Liu, Jeffrey C. Rapp