Abstract: A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.

Abstract: The present invention relates to the field of agriculture. In particular, the invention provides a promoter, a recombinant gene, plants comprising the recombinant genes and a method to improve yield of a cotton plant under stress conditions.

Abstract: The invention relates to the alfalfa variety designated R513M225S. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R513M225S. Also provided by the invention are tissue cultures of the alfalfa variety R513M225S and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R513M225S with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated R511Hg216. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R511Hg216. Also provided by the invention are tissue cultures of the alfalfa variety R511Hg216 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R511Hg216 with itself or another alfalfa variety and plants produced by such methods.

Abstract: Provided are isolated polynucleotides comprising a nucleic acid sequence encoding a polypeptide at least 80% identical to SEQ ID NO: 422, 362-421, 423-601, 2429-4085 and 4086, such as a polynucleotide which is at least 80% identical to SEQ ID NO: 260, 1-259, 261-361, 602-2427 and 2428, nucleic acid constructs comprising same, plant cells comprising same, transgenic plants expressing same, and methods of generating thereof for increasing the yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, nitrogen use efficiency and/or abiotic stress tolerance of a plant.

Abstract: The invention relates to the alfalfa variety designated RRL44M377. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety RRL44M377. Also provided by the invention are tissue cultures of the alfalfa variety RRL44M377 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety RRL44M377 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated RRL44M104. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety RRL44M104. Also provided by the invention are tissue cultures of the alfalfa variety RRL44M104 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety RRL44M104 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.

Abstract: Provided are methods of increasing yield, biomass, growth rate, vigor, and/or abiotic stress tolerance of a plant by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO: 84 or 921; or an exogenous polynucleotide encoding a polypeptide at least 80% identical to SEQ ID NO: 188.

Abstract: Polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The polypeptides of the invention have been shown to confer at least one regulatory activity and confer increased yield, greater height, greater early season growth, greater canopy coverage, greater stem diameter, greater late season vigor, increased secondary rooting, more rapid germination, greater cold tolerance, greater tolerance to water deprivation, reduced stomatal conductance, altered C/N sensing, increased low nitrogen tolerance, increased low phosphorus tolerance, or increased tolerance to hyperosmotic stress as compared to the control plant as compared to a control plant.

Abstract: The invention relates to the alfalfa variety designated R411K324. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R411K324. Also provided by the invention are tissue cultures of the alfalfa variety R411K324 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R411K324 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated RRL44M121. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety RRL44M121. Also provided by the invention are tissue cultures of the alfalfa variety RRL44M121 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety RRL44M121 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated R413A316. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R413A316. Also provided by the invention are tissue cultures of the alfalfa variety R413A316 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R413A316 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 1013T183. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 1013T183. Also provided by the invention are tissue cultures of the alfalfa variety 1013T183 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 1013T183 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated H0415A3144. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety H0415A3144. Also provided by the invention are tissue cultures of the alfalfa variety H0415A3144 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety H0415A3144 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 109T901. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 109T901. Also provided by the invention are tissue cultures of the alfalfa variety 109T901 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 109T901 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 914T031. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 914T031. Also provided by the invention are tissue cultures of the alfalfa variety 914T031 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 914T031 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 58M801. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 58M801. Also provided by the invention are tissue cultures of the alfalfa variety 58M801 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 58M801 with itself or another alfalfa variety and plants produced by such methods.

Abstract: A method for inoculating a plant with a nitrogen-fixing bacteria such as Gluconacetobacter diazotrophicus, said method comprising administering the nitrogen-fixing bacteria to a wound of a growing plant, for example to recently cut grass. Inoculation in this manner leads to enhanced growth characteristics including increased greenness of grass. Novel compositions suitable for use in the method are also described and claimed, together with kits for producing these.

Abstract: The invention relates to the alfalfa variety designated R411A106. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R411A106. Also provided by the invention are tissue cultures of the alfalfa variety R411A106 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R411A106 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated H0515A3140. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety H0515A3140. Also provided by the invention are tissue cultures of the alfalfa variety H0515A3140 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety H0515A3140 with itself or another alfalfa variety and plants produced by such methods.

Abstract: Pennycress seed, seed lots, and seed meal having reduced fiber content and improved suitability for use in producing animal feed are provided.

Abstract: The invention relates to the alfalfa variety designated 89T093. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 89T093. Also provided by the invention are tissue cultures of the alfalfa variety 89T093 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 89T093 with itself or another alfalfa variety and plants produced by such methods.

Abstract: A novel maize variety designated PH25RN and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH25RN with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH25RN through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH25RN or a locus conversion of PH25RN with another maize variety.

Abstract: The invention relates to the alfalfa variety designated 810T104. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 810T104. Also provided by the invention are tissue cultures of the alfalfa variety 810T104 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 810T104 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 412A121. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 412A121. Also provided by the invention are tissue cultures of the alfalfa variety 412A121 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 412A121 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 510M172. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 510M172. Also provided by the invention are tissue cultures of the alfalfa variety 510M172 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 510M172 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 413H323. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 413H323. Also provided by the invention are tissue cultures of the alfalfa variety 413H323 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 413H323 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 512W206. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 512W206. Also provided by the invention are tissue cultures of the alfalfa variety 512W206 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 512W206 with itself or another alfalfa variety and plants produced by such methods.

Abstract: A method of introducing an exogenous non-transcribable polynucleotide trigger, for example dsRNA, molecule into a seed is provided. The method comprises contacting the seed with the exogenous non-transcribable polynucleotide trigger, for example dsRNA, molecule under conditions which allow penetration of the exogenous non-transcribable polynucleotide trigger, for example dsRNA, molecule into the seed, thereby introducing the exogenous non-transcribable polynucleotide trigger, for example dsRNA, molecule into the seed.

Abstract: The invention relates to the alfalfa variety designated 510M374. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 510M374. Also provided by the invention are tissue cultures of the alfalfa variety 510M374 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 510M374 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 1114T029. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 1114T029. Also provided by the invention are tissue cultures of the alfalfa variety 1114T029 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 1114T029 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 1013M185. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 1013M185. Also provided by the invention are tissue cultures of the alfalfa variety 1013M185 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 1013M185 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated R410M324. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R410M324. Also provided by the invention are tissue cultures of the alfalfa variety R410M324 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R410M324 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The present disclosure provides compositions and methods for introducing nucleic acids into plants. More specifically, disclosed herein are methods and compositions for introducing a nucleic acid, such as a double-stranded RNA, for silencing a target gene in a plant. Also disclosed herein are plants exhibiting modification of a target gene, obtained through the use of such methods and compositions.

Abstract: Described are methods for promoting increase in plant biomass and yield. This increase has its visible effects in organs such as leaf, stem, root and production of fruits and seeds. Further described is the increase in tolerance of those plants to drought, generating plants better adapted to the environmental changes, improving their growth, biomass and yield.

Abstract: Disclosed herein are methods and compositions useful in targeting a payload to, or editing a target nucleic acid, where a governing gRNA molecule is used to target, optionally inactivate, a Cas9 molecule or a Cas9 molecule/gRNA complex.

Abstract: The invention relates to the alfalfa variety designated 412A122. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 412A122. Also provided by the invention are tissue cultures of the alfalfa variety 412A122 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 412A122 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 412W204. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 412W204. Also provided by the invention are tissue cultures of the alfalfa variety 412W204 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 412W204 with itself or another alfalfa variety and plants produced by such methods.

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

Abstract: Compositions and methods for the prevention and treatment of anxiety conditions associated with the use of cannabis are disclosed herein. The medicinal compositions include cannabidiol or cannabidiolic acid and one or more of the following compounds: bergamottin or dihydroxybergamottin, phloretin or a phloretin glycoside, piperine, apigenin or an apigenin glycoside and ursolic acid.

Abstract: The invention relates to the alfalfa variety designated 410A176. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 410A176. Also provided by the invention are tissue cultures of the alfalfa variety 410A176 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 410A176 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention provides siRNA compositions that specifically downregulates expression of a variant of the PNPLA3 gene and methods of use thereof for treating a chronic liver disease or alcoholic liver disease (ALD).

Abstract: Methods and compositions are described for producing a glucanase in transgenic plants and then incorporating parts of the transgenic plants in animal feed. The feed glucanase enzyme displays activity across a broad pH range, and tolerance to temperatures that are often encountered during the process of preparing animal feeds. Producing the enzyme in the transgenic plant enhances the thermal stability of the enzyme.

Abstract: Methods comprising DNA constructs and polynucleotides of functional transcription factors for improving photosynthetic capacity, biomass and/or grain yield and stress tolerance in various crop and model plants, dicots and monocots with the C3 or C4 photosynthetic pathways are described herein.

Abstract: This disclosure provides a number of sequences involved in axillary bud growth in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences or transgenes of such sequences, and tobacco products made from tobacco leaf harvested from such plants.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from Brassica napus.

Abstract: The present invention provides an expression cassette comprising a polynucleotide that encodes a protein that diverts a monolignol precursor from a lignin biosynthesis pathway in the plant, which is operably linked to a heterologous promoter. Also provided are methods of engineering a plant having reduced lignin content, as well as plant cells, plant parts, and plant tissues from such engineered plants.

Abstract: A polynucleotide having at least 80% sequence identity with the full-length nucleotide sequence of SEQ ID NO: 1 and substantially identical polynucleotides; an isolated polypeptide having at least 80% sequence identity with the full-length amino acid sequence of SEQ ID NO: 2 and substantially identical polypeptides; and polynucleotides encoding the Ha WRKY76 polypeptide and substantially identical polypeptides are described. Also described are vectors and recombinant expression cassettes containing the c DNA polynucleotide, a polynucleotide encoding the Ha WRKY76 polypeptide, or substantially identical polynucleotides. Transgenic plants containing such expression cassettes, related methods and uses are also provided.

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.