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  • Patent number: 9080178
    Abstract: The invention provides transgenic Escherichia coli cells comprising a mutation in cydAB gene, and/or cyoABCD gene, and/or cbdAB gene, and/or ygiN gene, wherein the mutation reduces (preferably, but not necessarily, by 100%) the cytochrome oxydase activity of a protein encoded by cydAB gene, and/or cyoABCD gene, and/or cbdAB gene, and/or ygiN gene. In a preferred embodiment, the mutation is a deletion of the cydAB gene, cyoABCD gene, and cbdAB gene. In another embodiment, the mutation is a deletion of the cydAB gene, cyoABCD gene, cbdAB gene, and ygiN gene.
    Type: Grant
    Filed: June 22, 2011
    Date of Patent: July 14, 2015
    Assignee: The Regents Of The University Of California
    Inventors: Bernhard Palsson, Vasiliy A. Portnoy
  • Publication number: 20120064581
    Abstract: The invention provides transgenic Escherichia coli cells comprising a mutation in cydAB gene, and/or cyoABCD gene, and/or cbdAB gene, and/or ygiN gene, wherein the mutation reduces (preferably, but not necessarily, by 100%) the cytochrome oxydase activity of a protein encoded by cydAB gene, and/or cyoABCD gene, and/or cbdAB gene, and/or ygiN gene. In a preferred embodiment, the mutation is a deletion of the cydAB gene, cyoABCD gene, and cbdAB gene. In another embodiment, the mutation is a deletion of the cydAB gene, cyoABCD gene, cbdAB gene, and ygiN gene.
    Type: Application
    Filed: June 22, 2011
    Publication date: March 15, 2012
    Inventors: Bernhard Palsson, Vasiliy A. Portnoy
  • Publication number: 20160228385
    Abstract: A purified cannabidiol (CBD) extract and/or cannabidiolic acid (CBDA) extract is isolated from industrial hemp and comprises less than 0.5 wt % organic impurities as measured by HPLC and 1H NMR spectroscopy exhibits no detectable peak at 4.07 ppm as measured by 1H NMR spectroscopy. The CBD and/or CBDA extract is in crystalline form. The CBD extract exhibits a melting point as measured by differential scanning calorimetry (DSC) of 69-70° C. Dry powder compositions comprise such extracts. Additional dry powder compositions comprise polyvinylpyrrolidone and an amorphous CBD extract. An adduct comprises CBD and/or CBDA bonded to a paramagnetic trivalent lanthanide (III) metal chelate.
    Type: Application
    Filed: February 5, 2016
    Publication date: August 11, 2016
    Applicant: COLORADO CAN LLC
    Inventors: Robert E. Sievers, Lia Rebits
  • Patent number: 10868915
    Abstract: A method is presented for linking a requestor to an agent of an entity. The method includes receiving a request to connect to an agent of an entity from a given requestor, retrieving a pool of agents from a data store, and determining a first subset of agents from the pool of agents, where agents in the first subset of agents have highest rating amongst agents in the pool of agents. The method also includes determining a second subset of agents from the pool of agents, where the agents in the second subset of agents have lowest number of assigned customers amongst the agents in the pool of agents. The method also includes identifying an intersection between the first subset and second subset, and assigning a dedicated agent to the given requestor from the intersection. The method also includes establishing a communication link between the given requestor and the dedicated agent.
    Type: Grant
    Filed: November 9, 2017
    Date of Patent: December 15, 2020
    Assignee: CBDA Holdings, LLC
    Inventors: Chris Buehler, Robert Duebelbeis, Brandon M. Brumage
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