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  • Publication number: 20130149752
    Abstract: The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.
    Type: Application
    Filed: November 19, 2012
    Publication date: June 13, 2013
    Applicant: EVOCATAL GMBH
    Inventor: EVOCATAL GMBH
  • Publication number: 20130230885
    Abstract: The present invention relates to the use of a protein comprising an LOV domain for the photosensitive defunctionalization of a molecule and to a method for the photosensitive defunctionalization of a target molecule.
    Type: Application
    Filed: August 16, 2011
    Publication date: September 5, 2013
    Applicant: EVOCATAL GMBH
    Inventors: Karl Erich Jaeger, Thomas Drepper, Stephan Endres, Janko Potzkei
  • Publication number: 20130280749
    Abstract: A FRET donor-acceptor pair for use as a biosensor, comprising at least two fluorescence proteins, wherein at least one fluorescence protein is stable with respect to a parameter to be detected by the biosensor and at least one fluorescence protein is unstable with respect to the parameter to be detected by the biosensor.
    Type: Application
    Filed: August 16, 2011
    Publication date: October 24, 2013
    Applicant: EVOCATAL GMBH
    Inventors: Karl Erich Jaeger, Thomas Drepper, Stephan Endres, Janko Potzkei, Achim Heck, Franco Circolone
  • Publication number: 20100304435
    Abstract: The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.
    Type: Application
    Filed: May 19, 2010
    Publication date: December 2, 2010
    Applicant: EVOCATAL GMBH
    Inventors: Thorsten Eggert, Sascha Hausmann, Michael Puls
  • Patent number: 8852882
    Abstract: A FRET donor-acceptor pair for use as a biosensor, comprising at least two fluorescence proteins, wherein at least one fluorescence protein is stable with respect to a parameter to be detected by the biosensor and at least one fluorescence protein is unstable with respect to the parameter to be detected by the biosensor.
    Type: Grant
    Filed: August 16, 2011
    Date of Patent: October 7, 2014
    Assignee: Evocatal GmbH
    Inventors: Karl Erich Jaeger, Thomas Drepper, Stephan Endres, Janko Potzkei, Achim Heck, Franco Circolone
  • Patent number: 8536314
    Abstract: The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.
    Type: Grant
    Filed: May 19, 2010
    Date of Patent: September 17, 2013
    Assignee: Evocatal GmbH
    Inventors: Thorsten Eggert, Sascha Hausmann, Michael Puls
  • Publication number: 20100112648
    Abstract: The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.
    Type: Application
    Filed: December 10, 2007
    Publication date: May 6, 2010
    Applicant: EVOCATAL GMBH
    Inventors: Thorsten Eggert, Jennifer Andexer
  • Publication number: 20140227698
    Abstract: To investigate protein-protein interactions, protein foldings and protein localization and also in the secretion of proteins, in vivo reporter proteins are used in biotechnology and in basic research. In order to be able to utilize fluorescence reporters as markers for secretion processes, FMN-binding fluorescence proteins (FbFP) have been developed by us for the first time. The new fluorescence markers can be expressed like GFP in various bacteria. The binding of the chromophore FMN produces a cyan-green fluorescent protein which can be detected in vivo using all customary spectroscopic and microscopic methods. In contrast to GFP, this protein can also surprisingly be secreted via the Sec route and be converted to the fluorescence-active state in the periplasma.
    Type: Application
    Filed: July 27, 2012
    Publication date: August 14, 2014
    Applicant: EVOCATAL GMBH
    Inventors: Thomas Drepper, Karl-Erich Jaeger, Janko Potzkei
  • Patent number: 8940516
    Abstract: The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.
    Type: Grant
    Filed: November 19, 2012
    Date of Patent: January 27, 2015
    Assignee: Evocatal GmbH
    Inventors: Thorsten Eggert, Jennifer Andexer
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