Search Patents
  • Publication number: 20030108952
    Abstract: Methods, reagents, and kits are provided that permit flow cytometric determination of the phosphorylation status of retinoblastoma susceptibility gene protein (pRB) in individual cells. Methods are described that permit the hypophosphorylated, active, form of pRB to be measured either as an absolute quantity or as a proportion of total cellular pRB. Further described are methods that permit pRB phosphorylation status to be correlated with cell cycle phase and with protein components of the cell cycle. Screening of chemical compounds for antiproliferative and antineoplastic activity using the flow cytometric assays is demonstrated. Reagent kits that facilitate the subject methods are also provided.
    Type: Application
    Filed: February 24, 1999
    Publication date: June 12, 2003
    Inventors: ZBIGNIEW DARZYNKIEWICZ, FRANK TRAGANOS, GLORIA JUAN, STEFAN GRUENWALD
  • Patent number: 7662565
    Abstract: Aspects of the invention concern methods for detecting, identifying and evaluating tobacco and tobacco products to determine the potential that these compositions have to contribute to a tobacco-related disease. It is based, at least in part; on the discovery that exposure of pulmonary cells to smoke or smoke condensate obtained from tobacco or tobacco products induces double stranded breaks in cellular DNA, which were efficiently detected using assays that measure the presence, absence, or amount of phosphorylation of the histone, H2AX.
    Type: Grant
    Filed: May 11, 2005
    Date of Patent: February 16, 2010
    Assignee: Vector Tobacco, Inc.
    Inventors: Anthony P. Albino, Ellen D. Jorgensen, Frank Traganos, Zbigniew Darzynkiewicz, Wendy Jin
  • Patent number: 7070943
    Abstract: Methods, reagents, and kits are provided that permit flow cytometric determination of the phosphorylation status of retinoblastoma susceptibility gene protein (pRB) in individual cells. Methods are described that permit the hypophosphorylated, active, form of pRB to be measured either as an absolute quantity or as a proportion of total cellular pRB. Further described are methods that permit pRB phosphorylation status to be correlated with cell cycle phase and with protein components of the cell cycle. Screening of chemical compounds for antiproliferative and antineoplastic activity using the flow cytometric assays is demonstrated. Reagent kits that facilitate the subject methods are also provided.
    Type: Grant
    Filed: September 29, 2004
    Date of Patent: July 4, 2006
    Assignee: Becton Dickinson and Company
    Inventors: Zbigniew Darzynkiewicz, Frank Traganos, Gloria Juan, Stefan Gruenwald
  • Patent number: 6821740
    Abstract: Methods, reagents, and kits are provided that permit flow cytometric determination of the phosphorylation status of retinoblastoma susceptibility gene protein (pRB) in individual cells. Methods are described that permit the hypophosphorylated, active, form of pRB to be measured either as an absolute quantity or as a proportion of total cellular pRB. Further described are methods that permit pRB phosphorylation status to be correlated with cell cycle phase and with protein components of the cell cycle. Screening of chemical compounds for antiproliferative and antineoplastic activity using the flow cytometric assays is demonstrated. Reagent kits that facilitate the subject methods are also provided.
    Type: Grant
    Filed: February 24, 1999
    Date of Patent: November 23, 2004
    Assignee: Becton, Dickinson and Company
    Inventors: Zbigniew Darzynkiewicz, Frank Traganos, Gloria Juan, Stefan Gruenwald